2012

May 20- 23, 2012 Nuremberg, Germany

www. IMCS2012.de

IMCS 2012 The 14th ~nternational Meeting

on Chem~cal Sensors

The 14th International Meeting on Chemical Sensors (IMCS-14) is the world's largest interdisciplinary forum on all aspects of chemical sensors encompassing physics, chemistry, materials science and engineering disciplines including biomedical engineering.

In 2012, IMCS is held jointly with the wo rld's largest sensor fair, the SENSOR+ TEST in Nuremberg.

Conference chair: Prof. Dr.- lng. Ralf M oos Department of Functional Materials University of Bayreuth

Venue: Nurnberg Messe

www.imcs2012.de info@imcs2012.de

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Booklet

IMCS 2012 - The 14th International Meeting on Chemical Sensors

This volume covers the proceedings of the IMCS 2012 - The 141h International

Meeting on Chemical Sensors. This volume comprises the abstracts and manuscripts of the lectures and poster presentations.

The authors are responsible for form and content of the papers. AMA Service GmbH accepts no responsibility for the correctness and completeness of the details and the consideration of private rights of third parties.

Publisher:

AMA Service GmbH P.O. Box 23 52 • 31506 Wunstorf I Germany Tel. +49 5033 9639-0 • Fax +49 5033 1056 info@sensor-test.com • www.sensor-test.com

ISBN 978-3-9813484-1-5

© AMA Service GmbH, 2012

IMCS 2012 - The 14th International Meeting on Chemical Sensors

1\ssociati<?n for Sensor ~Y'

Poster Session 2 1 - Biosensors

Tuesday, May 22, 2012 I 15:30-17:00 Entrance Mitte

DOl 1 0.5162/IMCS2012/P2.1.1

Express Biosensor control of maize plants under different agrotechnical procedures

R. Son 'ko1, 0. Marchenko1

, V. Kolomijez' 2, N. Starodub1

1 National University of Life and Environmental Sciences, Ukraine 2National Scientific Centre .,Institute of Agriculture", Ukraine

lt was used the developed portable optical biosensor ,.Fioratest" for the express control of state of the maiz plants at the different agrotechnical procedures. The induction of fluorescence of chlorophyl was served as the main registered index. lt was revealed the differences in the photosynthetic activities of plants in depending on soil preparation and nutrition by the fertilizer outside of root system.

DOl 1 0.5162/IMCS2012/P2.1 .2

Performance Study of a Potentiometric Sensor Array for Lactic Proteins Analysis

Lufs G. Dias\ Ana C.A. Veloso2·3

, Adelio A.S.C. Machado4, Ant6nio M. Peres1

'5

1 CIMO - Escola Superior Agraria, lnstituto Politecnico de Bragam;a, Portugal 2 DEQB - lnstituto Superior de Engenharia de Coimbra, Portugal 3 188 - Centre of Biological Engineering, University of Minho, Braga, Portugal 4 LAOUIPAI- Departamento Qufmica, Faculdade de Ci{mcias, Universidade do Porta, Portugal 5 LSRE- Laboratory of Separation and Reaction Engineering -Associate Laboratory LSRE/LCM, Escofa Superior Agraria, fnstituto Pofitecnico de Bragan9a, Portugal

Lactic proteins are complex biomolecules, possessing different molecular weights (from 19 kDa up to 66 kDa), which can be used as biomarkers for detecting food adulterations. This study reports for the first time the evaluation of two potentiometric sensor arrays, one electronic tongue (ET) using on ly cross-sensitivity chemical sensors and a hybrid-ET containing ionic-selective and cross-sensitivity chemical sensors. Although both devices could detect and distinguish soluble and insoluble lactic proteins, namely bovine serum albumin and three main casein fractions (alpha-, beta- and kappa-), the inclusion of ionic-selective sensors increased the device performance (100% of correct leave-oneout cross-validation classification for hybrid-ET against 94% for the ET) and reduced the number of sensors needed (5 for the hybrid-ET instead of 9 sensors used by the ET device). Finally, the results showed that with the hybrid-ET a second-order polynomial equation could be derived relating the measured potential of the aqueous solution and the logarithmic of the total casein concentration, with satisfactory determination coefficients (R2>0.99).

IMCS 2012 - The 14th International Meeting on Chemical Sensors 195

Luís G. Diasa, Ana C.A. Velosob,c, Adélio A.S.C. Machadod, António M. Peresa,e a CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança, Portugal; b DEQB – Instituto Superior de Engenharia de Coimbra, Coimbra, Portugal; c IBB – Centre of Biological Engineering, University of Minho, Braga, Portugal; d LAQUIPAI - Departamento Química, Faculdade de Ciências, Universidade do Porto, Portugal; e LSRE - Laboratory of Separation and Reaction Engineering - Associate Laboratory LSRE/LCM, Escola Superior Agrária, Instituto Politécnico de Bragança, Portugal.

ldias@ipb.pt; peres@ipb.pt !

Performance study of a potentiometric sensor array for lactic proteins analysis !

RESULTS 1 - QUALITATIVE ANALYSIS

4 different lactic proteins (bovine serum albumin (BSA), α-, β- and κ-caseins)

ê Sensor signals profile (40 sensors)

ê Stepwise – LDA

ê 5 Sensors selected

ê 99.8% of total variance explained (2 statistically significant

discriminant functions) ê

100% of correct classification of samples (Fig.3, leave-one-out cross-validation)

2 - QUANTITATIVE ANALYSIS Sensors’ signal to additions of increasing amounts

of total casein (0.04-2.0 g/L) ê

2nd order polynomial response (Fig. 4) ê

R2>0.99 (for several ion-selective and cross-sensibility sensors)

!6#

!3#

0#

3#

6#

9#

!40# !20# 0# 20# 40#

Function(2(

(5.5%(of(variance(explained)(

Function(1((94.4%(of(variance(explained)(

BSA# !CN# !CN# k!CN#

Fig. 3 Discriminant analysis obtained for standard aqueous solutions of

lactic proteins.

y = 0,0079x2 + 0,0311x + 0,1749 R! = 0,99679

0,14

0,15

0,16

0,17

0,18

0,19

-1,5 -1 -0,5 0 0,5

E(m

V)

Log(C, g/L) !

Fig. 4 Typical sensor response of the

potentiometric device to increasing

concentrations of total casein in aqueous

solutions.

IMCS 2012 - The 14th International Meeting on Chemical Sensors !May 20 - 23, 2012, Nürnberg/Nuremberg, Germany!

METHODOLOGY 40 ionic-selective and cross-sensibility chemical sensors applied to a potentiometric sensor array, constructed using a screen-printed technique (Fig. 1).

!

Scheme of the analytical multi-sensor system used for analysis with the potentiometric sensor array (Fig. 2).

Synthetic standard solutions (25ºC): - Individual standards of bovine serum albumin and α-, β- and κ-caseins (10-6-10-5 g/L). - Standard solution of total casein (20 g/L) Data treatment: Linear discriminant analysis (LDA)

SUMMARY Evaluation of a potentiometric chemical sensor array using:

Ion-selective and cross-sensibility sensors

ê 1 - Detect and distinguish soluble and insoluble lactic proteins

(bovine serum albumin and α-, β- and κ-caseins). Different molecular weights (from 19 kDa up to 66 kDa)

ê 2 - Evaluation of the response of the device versus increased

total casein concentrations in aqueous solutions

Fig. 1 – Potentiometric sensor array

Fig. 2 Multi-sensor

system

Preliminary results indicate that the potentiometric sensor array used has good analytical prospects within the protein analysis and may be used to detect biomarkers for evaluating food adulterations. MULTI-SENSOR DEVICE ADVANTAGE – No extraction or dilution/dissolution step was required. !

CONCLUSIONS: