TechniKrom, Inc. Bioprocess Columns - shin-han.comTechnikrom Inc. ˚ 1855 Elmdale Ave. ˚ Glenview, IL 60026 ˚ ˚ [email protected] 800-865-4100 ˚ International phone 847-556-9700

www.technikrom.com

TechniKrom, Inc.1855 Elmdale Ave.

Glenview, IL 60026 U.S.A.800-865-4100

800-293-5059 (fax)+1 847-556-9700 (int’l)

+1 847-556-9701 (int’l fax)www.technikrom.com

[email protected]

Bioprocess ColumnsFor purification of biopharmaceuticals

Warranty and Technical Support:: One year extendable warranty, service contracts and 24

hour response time means peace of mind and fast supportwhen you need it

:: Partner companies throughout Europe and Asia assist withoverseas tech support

Other TechniKrom Products andServices for the Bio, TIDES, and Pharma Industries::: Bioprocess LC Systems

:: IBDTM in-line buffer dilution skids with Adaptive PATTM

:: TechniPur™ chromatography packing materials

:: Process development, scale-up and tech transfer support

:: Equipment Validation (IQ/OQ and GAMP-5)

:: Service Contracts

:: Dynamic Axial Compression for rapid and reproducible packing of any media

:: Sanitary and sterilizable designs

“Designed by Chromatographers” ™

About TechniKromTechniKrom is a privately held, diversified engineering and qualification firmthat provides technologically advanced blending, synthesis, filtration andpurification equipment, products and services to the Biopharmaceutical,Pharmaceutical, and Nutraceutical Industries worldwide that permit thelowest cost of production and highest innate product quality, especially incGMP regulated environments. TechniKrom promotes the implementation of Manufacturing Science in biopharmaceutical manufacturing facilities. For additional information, please visit www.technikrom.com.

© Copyright TechniKrom Inc. ˚ www.technikrom.com ˚ 800-865-4100 ˚ TKBioProcColumnBroch6-08

Model # Product Specification Pressure Rating Operation Mode#LPLC-10-50-150psi 10cm i.d. x 50cm L 150psig/10 barg* Pneumatic#LPLC-10-70-150psi 10cm i.d. x 70cm L 150psig/10 barg* Pneumatic#LPLC-10-100-150psi 10cm i.d. x 100cm L 150psig/10 barg* Pneumatic

#LPLC-15-50-150psi 15cm i.d. x 50cm L 150psig/10 barg* Pneumatic#LPLC-15-70-150psi 15cm i.d. x 70cm L 150psig/10 barg* Pneumatic#LPLC-15-100-150psi 15cm i.d. x 100cm L 150psig/10 barg* Pneumatic


#LPLC-30-50-150psi 30cm i.d. x 50cm L 150psig/10 barg* Pneumatic or Hydraulic**#LPLC-30-70-150psi 30cm i.d. x 70cm L 150psig/10 barg* Pneumatic or Hydraulic**#HPLC-30-100-150psi 30cm i.d. x 100cm L 150psig/10 barg* Pneumatic or Hydraulic**

#LPLC-40-50-150psi 40cm i.d. x 50cm L 150psig/10 barg* Hydraulic*

#LPLC-60-50-150psi 60cm i.d. x 50cm L 150psig/10 barg* Hydraulic**





Standard Bioprocess Columns

:: Bed lengths can bepacked up toapproximately 65% ofthe column length

:: Worldwide install basethroughout US, Europe,and Asia

Dynamic Axial CompressionBioprocess LC Columns:: High-performance column packing

:: Eliminate voids and channels in the packed bed

:: CAD-modeled low shear “active” flow distribution provides highercolumn performance (lower HETP) and lower pressure drop than othercolumns and prevents direct flow disruption of the packed bed

:: Sterilizable designs available

:: “Hoist-free” operation – replace both frits without the use of anoverhead hoist or lift – designed for use in clean rooms orpurification suites with low overhead

:: Scalability – columns are scalable from 10 cm to 2m ID

:: Flexibility – varying bed heights can be packed in any column

:: Unique “open path” frit design is less susceptible to clogging and canbe replaced in minutes without liquid nitrogen chilling

:: Slurry inlet port for loading of slurry without removing column top orpiston

:: Slurry outlet ports for unpacking of soft gels and complete CIPwithout any column disassembly

:: No packing station required, no drop-down nozzles to interfere withflow distribution

:: Multipurpose design – suitable for affinity, ion exchange, HIC, or gelfiltration packing materials as well as large particle size normalphase or reverse phase packing materials

:: Suitable for protein purification or flash chromatography

:: Properly engineered columns for large-scale chromatography resultsin smooth, consistent piston movement

:: 316L stainless steel construction with highly fluorinated FDAcompliant seals that are compatible with all mobile phases

:: Electropolishing option available

:: FDA-compliant inert coating available for use with high chloride, lowpH applications

:: Jacketed options available for column temperature control

:: Internal leak detection option for seal quality assurance

:: Full material traceability for all stainless steel product contact parts

:: ASME code stamp option available

:: CE Pressure Equipment Directive compliance available

:: USP Class VI and TSE-free seal certification available

800-865-4100 ˚ International phone 847-556-9700Technikrom Inc. ˚ 1855 Elmdale Ave. ˚ Glenview, IL 60026 ˚ www.technikrom.com ˚ [email protected]

World class processing equipment and services for biopharm, pharma and fine chemical industries

Sterilizable Bioprocess Column

30cm LPLC Column

3D CAD modeling forBioprocess Columns

* Custom diameters,lengths and pressureratings are available

** Hydraulically drivencolumns include ahydraulic cart thataids in both dynamiccompression and hoist-free operation

HETP values achieved by end-user for different applications

Unpack

Ready to Use

Piston PackFill

Supports AllProcess LCMediaTypes: Ion exchange, Protein-A,affinity, size exclusion, reversephase, HIC.

HETP* dP media type

0.025cm 110mmdivinyl benzene(DVB) ionexchange

0.080cm 20mm C4 silica

0.003cm 15mmsphericalmonosized DVBresin

Column Operation

Model # Product Specification Pressure Rating Operation Mode#LPLC-10-50-150psi 10cm i.d. x 50cm L 150psig/10 barg* Pneumatic#LPLC-10-70-150psi 10cm i.d. x 70cm L 150psig/10 barg* Pneumatic#LPLC-10-100-150psi 10cm i.d. x 100cm L 150psig/10 barg* Pneumatic



#LPLC-30-50-150psi 30cm i.d. x 50cm L 150psig/10 barg* Pneumatic or Hydraulic**#LPLC-30-70-150psi 30cm i.d. x 70cm L 150psig/10 barg* Pneumatic or Hydraulic**#HPLC-30-100-150psi 30cm i.d. x 100cm L 150psig/10 barg* Pneumatic or Hydraulic**

#LPLC-40-50-150psi 40cm i.d. x 50cm L 150psig/10 barg* Hydraulic*






Standard Bioprocess Columns

:: Bed lengths can bepacked up toapproximately 65% ofthe column length

:: Worldwide install basethroughout US, Europe,and Asia

Dynamic Axial CompressionBioprocess LC Columns:: High-performance column packing

:: Eliminate voids and channels in the packed bed

:: CAD-modeled low shear “active” flow distribution provides highercolumn performance (lower HETP) and lower pressure drop than othercolumns and prevents direct flow disruption of the packed bed

:: Sterilizable designs available

:: “Hoist-free” operation – replace both frits without the use of anoverhead hoist or lift – designed for use in clean rooms orpurification suites with low overhead

:: Scalability – columns are scalable from 10 cm to 2m ID

:: Flexibility – varying bed heights can be packed in any column

:: Unique “open path” frit design is less susceptible to clogging and canbe replaced in minutes without liquid nitrogen chilling

:: Slurry inlet port for loading of slurry without removing column top orpiston

:: Slurry outlet ports for unpacking of soft gels and complete CIPwithout any column disassembly

:: No packing station required, no drop-down nozzles to interfere withflow distribution

:: Multipurpose design – suitable for affinity, ion exchange, HIC, or gelfiltration packing materials as well as large particle size normalphase or reverse phase packing materials

:: Suitable for protein purification or flash chromatography

:: Properly engineered columns for large-scale chromatography resultsin smooth, consistent piston movement

:: 316L stainless steel construction with highly fluorinated FDAcompliant seals that are compatible with all mobile phases

:: Electropolishing option available

:: FDA-compliant inert coating available for use with high chloride, lowpH applications

:: Jacketed options available for column temperature control

:: Internal leak detection option for seal quality assurance

:: Full material traceability for all stainless steel product contact parts

:: ASME code stamp option available

:: CE Pressure Equipment Directive compliance available

:: USP Class VI and TSE-free seal certification available

800-865-4100 ˚ International phone 847-556-9700Technikrom Inc. ˚ 1855 Elmdale Ave. ˚ Glenview, IL 60026 ˚ www.technikrom.com ˚ [email protected]

World class processing equipment and services for biopharm, pharma and fine chemical industries

Sterilizable Bioprocess Column

30cm LPLC Column

3D CAD modeling forBioprocess Columns

* Custom diameters,lengths and pressureratings are available

** Hydraulically drivencolumns include ahydraulic cart thataids in both dynamiccompression and hoist-free operation

HETP values achieved by end-user for different applications

Unpack

Ready to Use

Piston PackFill

Supports AllProcess LCMediaTypes: Ion exchange, Protein-A,affinity, size exclusion, reversephase, HIC.

HETP* dP media type

0.025cm 110mmdivinyl benzene(DVB) ionexchange

0.080cm 20mm C4 silica

0.003cm 15mmsphericalmonosized DVBresin

Column Operation

www.technikrom.com

TechniKrom, Inc.1855 Elmdale Ave.

Glenview, IL 60026 U.S.A.800-865-4100

800-293-5059 (fax)+1 847-556-9700 (int’l)

+1 847-556-9701 (int’l fax)www.technikrom.com

[email protected]

Bioprocess ColumnsFor purification of biopharmaceuticals

Warranty and Technical Support:: One year extendable warranty, service contracts and 24

hour response time means peace of mind and fast supportwhen you need it

:: Partner companies throughout Europe and Asia assist withoverseas tech support

Other TechniKrom Products andServices for the Bio, TIDES, and Pharma Industries::: Bioprocess LC Systems

:: IBDTM in-line buffer dilution skids with Adaptive PATTM

:: TechniPur™ chromatography packing materials

:: Process development, scale-up and tech transfer support

:: Equipment Validation (IQ/OQ and GAMP-5)

:: Service Contracts

:: Dynamic Axial Compression for rapid and reproducible packing of any media

:: Sanitary and sterilizable designs

“Designed by Chromatographers” ™

About TechniKromTechniKrom is a privately held, diversified engineering and qualification firmthat provides technologically advanced blending, synthesis, filtration andpurification equipment, products and services to the Biopharmaceutical,Pharmaceutical, and Nutraceutical Industries worldwide that permit thelowest cost of production and highest innate product quality, especially incGMP regulated environments. TechniKrom promotes the implementation of Manufacturing Science in biopharmaceutical manufacturing facilities. For additional information, please visit www.technikrom.com.

© Copyright TechniKrom Inc. ˚ www.technikrom.com ˚ 800-865-4100 ˚ TKBioProcColumnBroch6-08

Documents

TechniKrom, Inc. Bioprocess Columns - shin-han.comTechnikrom Inc. ˚ 1855 Elmdale Ave. ˚ Glenview, IL 60026 ˚ ˚ [email protected] 800-865-4100 ˚ International phone 847-556-9700