Supplementary Table 1. Subcellular distribution of wild-type and mutant POP-1 in mammalian cells.

Subcellular localization

POP-1 proteins LMB N > C (%) N = C (%) N < C (%)

wt - 45.6 ± 3.4 22.3 ± 3.9 32.1 ± 1.9

wt + 93.0 ± 3.3 3.7 ± 2.2 3.2 ± 1.1

MutAAA - 1.0 ± 0.6 1.1 ± 0.2 97.8 ± 0.4

MutAAA + 16.8 ± 4.1 10.5 ± 0.7 72.6 ± 4.0

MutRRR - 1.5 ± 0.9 2.1 ± 0.7 96.4 ± 1.4

MutRRR + 16.3 ± 3.2 12.8 ±3.1 70.8 ± 5.5

NLS::wt - 68.2 ± 3.9 18.7 ± 2.8 13.0 ± 3.9

NLS::wt + 86.3 ± 0.9 8.8 ± 1.4 4.8 ± 1.3

NLS::MutAAA - 2.6 ± 0.5 3.8 ± 0.4 93.5 ± 0.7

NLS::MutAAA + 85.1 ± 3.4 8.0 ± 1.7 6.8 ± 1.7

NLS::MutRRR - 1.9 ± 0.5 3.7 ± 1.0 94.3 ± 1.5

NLS::MutRRR + 84.5 ± 1.5 8.4 ± 2.0 7.0 ± 0.4

The indicated POP-1 proteins were expressed in COS-7 cultured in the absence or presence of Leptomycin B (LMB), and were detected by immunofluorescence using the monoclonal -POP-1 antibody. The percentage of cells with clearly visible nuclear over cytoplasmic staining (N > C), evenly distributed signal in both nucleus and cytoplasm (N = C), or predominantly cytoplasmic staining (N < C) are shown. Results are expressed as the average and standard deviation of the values obtained in three independent experiments. For each experiment, 1000 cells were scored.

Gay et al.,