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STARCH-SUCROSE METABOLISM
Dr.Roshni Rajamohan
Department of Botany
Deshbandhu College
Dr. Roshni Rajamohan, Deshbandhu College
Carbohydrates
• Plants are autotrophs and synthesize carbohydrates through photosynthesis, then use them to create biological macromolecules.
• Plant‐derived carbohydrates (e.g., cellulose) dominate the biosphere and serve as a key sink for atmospheric carbon dioxide (CO2).
• The primary use of the captured energy is the fixation and reduction of CO2via the Calvin–Benson cycle.
Dr. Roshni Rajamohan, Deshbandhu College
Carbohydrates
• Plants store carbs as – vacuolar sucrose or fructans
-but the most common storage form is Starch, a polymer of Glucose
- bacteria, animals and fungi- store as glycogen, much branched
polymer of glucose
Starch – chloroplast synthesized during day in chloroplasts stroma in photosynthetic tissues
stored temporarily until night
degraded at night
In storage tissues, such as tubers or seeds, starch is synthesized in amyloplasts and stored for much longer periods
Dr. Roshni Rajamohan, Deshbandhu College
Three interconvertible hexose phosphates make up the hexose phosphate pool
Dr. Roshni Rajamohan, Deshbandhu College
Triose PO4 pool to Hexose PO4 pool
• DHAP & 3-PGAL–Triose PO4 pool
• Aldolase condensation
• Hexose pool
Fr-1, 6- PO4
Fr- 6- PO4
Gl- 6- PO4
Gl- 1 - PO4
Fr-1, 6- Phosphatase
Hexose Isomerase
Phospho gluco -Mutase
Dr. Roshni Rajamohan, Deshbandhu College
Starch synthesis
• Starch synthesis involves chain elongation, branching, and debranching reactions
• The first committed step in the starch biosynthetic pathway is the production of ADP‐glucose by ADP‐glucose pyrophosphorylase
• Glucose-1-phosphate + ATP -----------> ADP-glucose + Ppi
(imported from cytosol)
• Three enzymes are responsible for the synthesis of starch from ADP‐glucose:
1. starch synthases
2. starch branching enzymes- produces the α‐1,6 branches in starch
3. starch debranching enzymes
In the transport processes illustrated here, photosynthetic chloroplasts export triose phosphate in exchange for inorganic phosphate (Pi); this is a major flux in photosynthesis. Transporters also exchange pentose phosphates and phosphoenolpyruvate for Pi, and nonphotosynthetic plastids also have transporters mediating the movement of hexose phosphates . Adenylate cofactors can also be transported across the chloroplast envelope, as can maltose and glucose (the products of starch breakdown) and intermediates involved in nitrogen assimilation. All the transporters are reversible, and the direction of transport depends on the concentrations of metabolites on each side of the envelope
Transporters located in the inner envelope membranes of plastids exchange metabolites between the cytosol and the plastid stroma.
Dr. Roshni Rajamohan, Deshbandhu College
STARCH
• Osmotically inert form of carbohydrate –polymer of GLUCOSE
• Massive, compact, stable, insoluble semi-crystalline granules
• Starch granules contain 2 distinct glucose polymers-
Amylose and Amylopectin
both of which are homopolymers of α‐1,4‐linked glucose
• Why starch?
• If a comparable number of hexose units accumulated in the plastid as sucrose, the stromal solution would contain too many solute particles, and water from the cytosol would flood in by osmosis, causing the plastid to swell and burst
Dr. Roshni Rajamohan, Deshbandhu College
Dr. Roshni Rajamohan, Deshbandhu College
COMPONENTS OF STARCH
Amylopectin• Branched molecule• α-(1,4)- and α-(1,6) linkages
(branch points)• branching pattern is not random-
one branching point per 24 to 30 glucose residues
• 1,00,000-10,00,000 glucose residues
• 70% or more of the starch• similar to glycogen
Amylose
• a linear polymer of glucose• An α-(1,4)-glucan• smaller than amylopectin• has very few branch points• 1,000-20,000 glucose residues• Accounts• 30% or less of the starch
Dr. Roshni Rajamohan, Deshbandhu College
Starch Synthesis• EnzymesADP-glucose pyrophosphorylase and starch synthase—
are found localized in the chloroplast stroma.
• Starch synthesis in the chloroplast begins with the hexose phosphate pool generated by the PCR cycle
Fructose-6-P < ---------------------------------------------------→ Glucose -6-Phexose-phosphate isomerase
Glucose -6-P < ----------------------------------------→ Glucose -1-Pphosphoglucomutase
ATP + Glucose-1-P -----------------------------------→ ADP-Glucose + H2O + PPiADP-glucose phosphorylase
PPi + H2O ↔ 2Pi
ADP-Glucose + α-(1→4)-glucan ---------------------→ α-(1→4)-glucosyl-glucan + ADP
Starch synthase
Branching enzyme(Q Enzyme)- Give rise to → α-(1→6) branching of amylopectin
Debranching Enzymes- trims the side chains and make amylopectin ready for package into starch granuleand provides primers (α-(1→4)-glucan ) for ADP-Glucose addition to their non-reducing end
The aldehyde group can react with (reduce) organic and inorganic substrates and is, therefore, described as the reducing end of the molecule.Eg.Glucose, Fructose
Reducing end Non-reducing end
ADP-Glucose +
During the day, carbon dioxide (CO2) is assimilated through photosynthesis. Some is used for growth, and some is stored. At night, stored carbohydrate is used to support respiration and continued growth.
Dr. Roshni Rajamohan, Deshbandhu College
Sucrose biosynthesis
• Sucrose is a major product of photosynthesis in green leaves and accounts for much of the CO2fixed during photosynthesis.
• It also serves as the principal long‐distance transport compound in most plants and as a storage compound in some [including sugar beet (Beta vulgaris), sugar cane (Saccharum sp.), and carrot (Daucus carota)].
Fate of sucrose in sink tissues
Sucrose needs to be metabolized or stored
Why ?
Fate of sucrose
1. It can be respired.2. It can be stored as sucrose, starch or another carbohydrate, or converted to a lipid.3. It can be used for growth and the synthesis of cellcomponents.
During sucrose synthesis, the anomeric carbon of fructose (C‐2) is joined to the anomeric carbon of glucose (C-1) by a glycosidic bond. This bond protects the reducing ends of both monomers
By joining the carbonyl carbons of glucose and fructose in a stable glycosidic bond, sucrose formation prevents these groupsfrom becoming oxidized through non
enzymatic reactions with other cellular components and best suitable as a transport molecule.
Sucrose is a nonreducing sugarGlucose + Fructose = Sucrose
Dr. Roshni Rajamohan, Deshbandhu College
• Uridine diphosphate‐glucose(UDP‐glucose is one of the two substrates required for sucrose synthesis (the other is fructose‐6‐phosphate)
• Glucose-1-phosphate + UTP -------------------------→ UDP-glucose + Ppi
• UDP-glucose +Fructose-6-phosphate<------> Sucrose-6-phosphate + UDP
• Sucrose-6-phosphate+ H2O --------- > Sucrose + Pi
UDP‐glucose pyrophosphorylase
Sucrose‐phosphate synthase
Sucrose-phosphate phosphatase
PPi + H2O ↔ 2 Pi
Dr. Roshni Rajamohan, Deshbandhu College
UDP-glucose +Fructose-6-phosphate <------> Sucrose-6-phosphate+ UDP
Sucrose‐phosphate synthase is subject to allosteric modulation by glucose 6‐phosphate--- which activates the enzyme by Pi , which inhibits it.
Sucrose‐phosphate synthase
Dr. Roshni Rajamohan, Deshbandhu College
• The formation of sucrose by this route has a large negative free energy change (ΔGo′= –25 kJ mol–1), which renders this sequence of reactions is not spontaneous and is essentially irreversible in vivo.
• Under normal conditions SS operates in the reverse direction to break down sucrose
Sucrose synthase
Sucrose synthaseSucrose + UDP ------------------------→ Fructose + UDP−glucose
UDP-glucose + fructose --------------→ Sucrose + UDP
UDP-glucose + PPi -------------------------------------→ UTP + glucose-1-PUDP−glucose pyrophosphorylase
Glucose -1-P + ATP -------------------------------→ ADP-glucose + H2O+ PPi
ADP−glucose phosphorylase
ADP-Glucose + α-(1→4)-glucan ---------------------→ α-(1→4)-glucosyl-glucan (STARCH) + ADP
Starch synthase
Dr. Roshni Rajamohan, Deshbandhu College
SUCROSE DEGRADATION• Sucrose is transported through symplastic or apoplastic pathways to
the sink tissues where it is broken down.
• Sucrose can be degraded by either Sucrose synthase or Invertase
The reaction catalyzed by invertase is irreversible and so leads only to sucrose degradation.
Sucrose synthase catalyzes a reversible reaction that can synthesize or degrade sucrose. In plant cells, this enzyme is associated primarily with sucrose degradation.
Dr. Roshni Rajamohan, Deshbandhu College
Hexokinase uses ATP to phosphorylate glucose and fructose, the products of invertase. Sucrose synthase and UDP‐glucose pyrophosphorylaseact together to generate hexose phosphate by an ATP‐independent pathway
Both enzymes often appear in the same tissues. In addition, both sucrose synthase and invertase occur as multiple isoenzymes, each of which probably functions in a specific tissue or cellular compartment.It is considered that sucrose synthase activity tends to be associated with the utilization of sucrose for the biosynthesis of non-structural carbohydrate polymers like starch, and of structural carbohydrate polymers like cellulose and callose
Dr. Roshni Rajamohan, Deshbandhu College
• Invertases - acid invertases (acidic pH optima) or alkaline invertases (neutral or alkaline pH optima).
• Operate in different cellular compartments:
Acid invertases are present in both the vacuole and the apoplst
Vacuolar invertase- hydrolyzes sucrose stored in the vacuole, with
the subsequent transport of the free hexoses to the cytosol for
metabolism. Eg., developing fruits, and in rapidly growing tissues, such
as the elongation zones of roots and hypocotyls
Apoplastic invertase - is bound firmly to the cell wall matrix and
hydrolyzes apoplastic sucrose delivered to sink tissues. plant reproductive tissues. Repression of cell wall
invertases interferes with pollen development, causing male sterility and decreased seed size in
important crops like maize (Zea mays) and rice (Oryza sativa)
Alkaline invertases are present in the cytosol and plastid.
Cytosolic invertases are also important for plant growth, particularly roots
Plants also possess proteinaceous invertase inhibitors that interact with the acid invertases of the apoplast and the vacuole. These inhibitors likely repress invertase activity
INVERTASE TYPES