PowerPoint PresentationFood-borne Zoonoses and Antimicrobial Resistance Unit
VISAVET Health Surveillance Centre, UCM
Birmingham, UK
Garayzabal, Ana Mateos and Lucas Domínguez
Methicillin resistant S. aureus
February 2005, 2 new cases of MRSA were identified, one in
a pig farmer from a different region and one in the son of a
veterinarian who worked mostly with pigs.
In July 2004, we unexpectedly found MRSA in the
preoperative screening cultures of a 6-month-old girl before
thoracic surgery.
http://www.olmix.com/animal-division/pigs
de Neeling et al.,
t034 (n = 9) and
t1793 (n = 1) ST398
t011, t108, t567,
VAV Network
Monitoring Antimicrobial Resistance in bacteria from animal origin in Spain
• At slaughterhouse
• Slaughterhouse selection
• Randomly selected batches • One sample/batch • One batch/farm • Samples: skin swabs (ear)
Monitoring programmes
• Short term evolution
08 16 02 25 34 24 25 GCACCAAAA TACATGTCGT t011
08 16 02 25 GCACCAAAA TACATGTCGT 34 25 t1451
08 16 02 25 02 25 34 24 25 GCACCAAAA TACATGTCGT t034
07 23 21 16 34 33 13 GCACCAAAA TACATGTCGT t127
Characterization
arcC Carbamate kinase
Allelic profile Long term evolution
www.mlst.net
Characterization
• 2009
t011 t034 t108 t1197 t1255 t127 t1451 t4872 t588
Fattening pigs at slaughterhouses
130
t127 1 0 0
t1344 1 0 0
t1451 5 0 1
t1456 2 0 0
t4871 0 1 0
t4872 1 0 0
t4873 1 0 0
t4874 1 0 0
t4884 1 0 0
t4885 1 0 0
t693 1 0 0
t899 1 0 0
Small ruminants
griffon vulture TOTAL
Nasal swabs 269 211 795 40 1315
Skin swabsa 274 154 797 1225
Otherb 4 4
NDc 2 2
Tested animals 273 212 817 40 1342
a. Skin swabs were taken from retro-auricular area in red deer and wild boar and from inguinal-mammal area in Iberian ibex; b.
Exceptionally, Iberian ibex were sampled on ear (n=2) and retro-auricular skin (n=2); c. ND: not determined
Eurasian griffon vulture
Red deer Iberian ibex Wild boar Eurasian griffon
vulture TOTAL
Positive
animals %
(CI)
1
0.37
(0.01,2.02)
2
0.94
(0.11,3.37)
7
0.86
(0.35,1.76)
2
5.00
(0.61,16.92)
12
0.89
(0.46,1.56)
Genotype (n) ST398-t011 (1) ST398-t011 (1) ST398-t011 (6) ST398-t011 (2)
ST398-t1451 (1) ST1-t127 (2)
Eurasian griffon vulture Iberian ibex Wild boar Red deer
Livestock (n=39)
Cattle (n=5)
Fallow deer (n=2)
Iberian ibex (n=39)
Expanding the presence of Staphylococcus aureus carrying mecC
gene: Spanish emergence in wild animals and urban effluents M. Concepción Porreroa, Arancha Valverdea, Pedro Fernández-Llariob, Alberto Díez-
Guerriera, Ana Mateosa, Santiago Lavínc, Rafael Cantónd, José-Francisco Fernández-
Garayzabala, and Lucas Domíngueza,* mecC
Swabs/Water samples
Identification (PCR)
Fallow deer
Urban effluents
*MLST: Multi Locus Sequence Typing. †CC: Clonal Complex. ‡AST: Antimicrobial Susceptibility Testing.
§PEN: Benzylpenicillin. ¶FOX: Cefoxitin.
mecA mecC n % n % n
Wild animal Griffon vulture 2 0 2 50,0 2 50,0 4
Fallow deer 0 1 1 100,0 0 0,0 1
Iberian ibex 2 0 2 5,3 36 94,7 38
Mouflon 0 0 0 0,0 2 100,0 2
Red deer 1 0 1 1,7 57 98,3 58
Wild boar 8 1 9 6,2 136 93,8 145
Livestock Cattle 7 0 7 58,3 5 41,7 12
Fattening pigs 178 0 178 86,4 28 13,6 206
Urban water Urban effluent 104 1 105 61,0 67 39,0 172
MRSA: methicillin resistant S. aureus; MSSA: methicillin susceptible S. aureus
MRSA vs MSSA • n=635 isolates
UW MSSA WA MSSA LA MSSA UW MRSA WA MRSA LA MRSA
S im
10 12
10 12
0
20
40
60
80
S T 5
S T 1
S T 2 6 7 1
S T 2 6 3 7
S T 1 6 4 3
S T 3 5 0
S T 5 2 2
S T 1 3 0
S T 2 6 8 1
S T 3 9 8
S T 1 8 8
S T 2 6 3 9
S T 2 6 4 0
S T 2 6 7 5
S T 1 5
S T 3 0
S T 9 6
0
20
40
60
80
MRSA_mecA MRSA_mecC
0 5
SCCmec
MRSA
animals MSSA