STANDARDIZATION OF PAEDERIA FOETIDA EXTRACT FOR DEVELOPING ...dbtepromis.gov.in/.../ProposalID_4528_040116_KSUDBSC_DBT_Proje… · r&d project proposal on standardization of paederia

R&D PROJECT PROPOSAL

ON

STANDARDIZATION OF PAEDERIA FOETIDA EXTRACT

FOR DEVELOPING A HERBAL FORMULATION TO TREAT

PEPTIC ULCER

Principal Investigator

Dr. Kuldeep Singh

Co-Investigators

Mr. Udayabanu M.

Ms. Silpi Chanda

Jaypee University of Information Technology

Waknaghat, Solan-173215 (H.P.)

2

PROFORMA – I

PROFORMA FOR SUBMISSION OF PROJECT PROPOSALS ON RESEARCH AND

DEVELOPMENT, PROGRAMME SUPPORT

PART I: GENERAL INFORMATION

1. Name of the Institute/University/Organisation submitting the Project Proposal:

Department of Biotechnology , Bioinformatics & Pharmaceutical Sciences, Jaypee University of

Information Technology, Waknaghat, P.O. Dumehar Bani, Kandaghat, District Solan-173215

(H.P.) INDIA

2. State: Himachal Pradesh

3. Status of the Institute: State University

4. Name and designation of the Executive Authority of the Institute/University forwarding

the application: Dr. Ravi Prakash, Vice-Chancellor, Jaypee University of Information

Technology (HP)

5. Project Title: STANDARDIZATION OF PAEDERIA FOETIDA EXTRACT FOR

DEVELOPING A HERBAL FORMULATION TO TREAT PEPTIC ULCER

6. Category of the Project (Please tick): R&D Support

7. Specific Area: Aromatic and Medicinal Plant

8. Duration: 3 Years

9. Total Cost (Rs.): 47.128 lakhs + Overheads As applicable

10. Is the project Single Institutional or Multiple-Institutional (S/M)? : S

12. Scope of application indicating anticipated product and processes

1. Studies will result in the standardization, chemical modification and formulation of a

suitable herbal formulation for peptic ulcer.

2. Exploration of chemically engineered extracts would be a useful resource of chemical

library of natural products with potential applications.

3. The prepared herbal formulation will not only be a remedy for PUD patients but will also

target patients who regularly consume NSAIDs especially in case of arthritic and post

operative patients.

13. Project Summary

Paederia foetida Linn. (Rubiaceae), known as ‗Prasarini‘ in Sanskrit is an extensive foetid smell

climber. It has been used for various purposes in the Indian systems of medicine as well as in

folk-lore medicine (Table 2). The tribal community of Northeast part of India and other parts of

Asia including Bangladesh use this plant mainly for gastro intestinal diseases. Our aim is to find

out a most active standardized fractional extract which could be formulated for the treatment of

peptic ulcer disease (PUD). Chemical modification of the most active fraction will also be done

with an aim to increase the potency/efficacy of the extract.

3

The project emphasizes on the Indigenous System of Medicine, mainly the study of plants used

by the tribal people of North-Eastern region of India as a food as well as medicine. To the best

of our knowledge, no systematic study has been done in the field of standardization and

formulation of herbal preparation with the chemical modification.

The approach involves extraction of plant material with various solvents and their profiling for

anti-ulcer activity. We will use different models for this study (described later in this proposal).

Bio-guided fractionation will be used to identify active fractions. Plant extracts have synergistic

effects of various molecules present and our aim is to enrich them by bio-guided fractionation

method. We plan to obtain a biological fingerprint of each extract step by step through

pharmacological evaluation of their anti ulcer activity.

While we are in the search of active fractions, we would also do chemical modification of

natural molecules present in such fractions. We would employ a methodology —Chemically

Engineered Extracts (CEE) — for chemical diversification.1 Natural extracts like libraries will

be generated by using CEE method.

Expected outcome of this project is to obtain a standardized extract, to have enriched fraction

with chemical modification and formulation of that bioactive enriched fraction as herbal

formulation that could be used for peptic ulcer disease.

4

PART II: PARTICULARS OF INVESTIGATORS

14. Name Dr. Kuldeep Singh

Date of Birth 01/11/1978

Designation Senior Lecturer

Department Biotechnology, Bioinformatics and Pharmaceutical Sciences

Institute/University Jaypee University of Information Technology

Address Waknaghat, P.O. Dumehar Bani, Kandghat, District

Solan- (H.P.) INDIA, PIN: 173215

Telephone 01792-239391

Fax: 01792-245362 E-mail: [email protected]

Number of research projects being handled at present: None

Co-Investigators

15a. Name Mr. Udayabanu M.

Date of Birth 01/09/ 1977

Designation Lecturer




Solan- (H.P.) INDIA PIN: 173215



Number of Research projects being handled at present: One

15b. Name Ms. Silpi Chanda

Date of Birth 13/03/1979

Designation Assoc. Lecturer




Solan- (H.P.) INDIA PIN: 173215



Number of Research projects being handled at present: None

5

PART III: TECHNICAL DETAILS OF PROJECT

16. Introduction

16.1 Origin of the proposal

―Let your food be your medicine‖ exclaimed Hippocrates. Herbs, spices and other nutritional

substances are the oldest form of medicine known to man. The relationships between the human

being and plants are very close throughout the development of human health care system. A

large number of plants used in the traditional practice have now become a part of the modern

health care system and have been widely explored as supplements, and/or as alternatives to

conventional medicine.2 However, foods being exceedingly complex packages of chemicals and

compounds, do not deliver a single biological effect, as do pharmaceutical drugs designed to

accomplish a specific purpose. Ethno-botanical studies in India and abroad have revealed that

there is large scope for the development of new drugs from plants especially those used by

tribals are yet unexplored. So evaluation of ethno-botanical plants, preparation and

standardization of the extract or isolation of new chemical substances from tribal plants could be

exploited to identify and develop drug like molecules, which will not only be cost effective but

comparatively safer than the modern medicines.

In view of the importance of ethno-medicinal plants, an extensive field survey was done among

the tribal family residing in and around Agartala to identify valuable plants which have common

use among the various tribal communities for the treatment of gastrointestinal ulcer. The survey

indicated that out of nineteen tribal communities, eleven were using Paederia foetida for gastro

intestinal diseases — which seems to be peptic ulcer by characteristics described by tribal

communities. Paederia foetida is one of the tribal plants used as edible as well as medicine for

gastric related problems in North–East region of India and other parts of Asia. A literature

review showed that this herb has also been used in gastritis in various parts of world.3 The plant

has been described in Ayurvedic Pharmacopoeia of India4 and in Wealth of India

5.

Paederia foetida is one of the ingredients of different Ayurvedic formulations viz.

Dasamularista, Mahanarayana oil, Sudard (used as analgesic and anti-inflammatory), Prasarini

Taila (used gout), Kubja Prasarini Taila etc.

The present study is proposed for bioguided fractionation to identify and isolate the marker

compound(s) and to formulate a herbal preparation after doing chemical modifications with the

aim to increase its activity against PUD.

16.2 Rationale of the study supported by cited literature

Gastrointestinal (GI) disorders are set to affect over 250 million people in the seven largest

pharma markets, by 2012.6 Among all GIT disorders, peptic ulcer is one of the most crucial

issue in intensive care units where critically ill patients have many known risk factors. At

present, the pharmaceutical prescription market to treat GI disorders is valued at just over $20

billion, reflecting that it is one of the largest and most important therapeutic areas. World wide

India has 5th

rank in peptic ulcer disease (PUD) caused deaths (death rate 12.37/Lakh

population) and in India it is the 18th

most common cause of death (1.20%).7 Though peptic

ulcer of stomach and duodenum was known in 1700s and there is revolution of understanding

and management of PUD after the discovery of H. pylori. PUD represents a world wide health

6

problem because of its high mortality and morbidity. The Mortality (min 4 & max 30 %) and

Morbidity (min 25 & max 89%) varies as it‘s a multifactorial disease. 8 5 - 15% of adult

population of world is suffering with PUD.9 Based on type of mechanism of action, a number of

treatments are available for peptic ulcer — eg. Use of Antacids, H2 blockers, Proton pump

inhibitors, antibiotic, and combination therapy (Figure 1).10

ProglumideACh

PGE2

HistamineGastrin

Adenyl

cyclase

_+

ATP cAMP

Protein Kinase(Activated)

Ca++

+

Ca++

Proton pump

KK+ H+

Gastric acid

Parietal cell

AntacidOmeprazole

Ranitidine

H2M3

Misoprostol

__

+

PGE receptor

+

+

+

+

Gastrin

receptor

__ Lumen of stomach

+

Figure 1 Different mechanism of action for treatment of PUD

But each category is having unavoidable limitations for example long term use of H2 blockers

cause hip fracture.11

High doses or long-term use of Proton pump inhibitors (PPIs) carry a

possible increased risk of bone fractures.12

Although PPIs are well-tolerated, there have been

reports and recent cases implicating acute interstitial nephritis (AIN) and progression to acute

renal failure (ARF).13

The other side effects of PPIs are osteoporosis, hepatitis, visual

disturbance14

. FDA approved double, triple & quadruple therapy (Table 1) also causes

treatment failure and the main reason is patient noncompliance due to concomitant use of

medicine.

Patients have to follow the combination therapy, as each category of medicine is having

different mechanism of action. Hence a single drug treatment fails to meet the need. For the

cheaper option if any category is removed, it leads to reduction in efficacy.

In this aspect, herbal formulation may be a better option as it might act through multiple

mechanisms of action. Herbal medicines have emerged as a unique approach for meeting the

need for safe, effective and relatively inexpensive new remedies.

7

Table 1: FDA approved double, triple & quadruple therapy

Category Times to take Duration (days) Total no. of tablet

Proton pump inhibitor + 2

14 196 Bismuth subsalicylate 525mg + 4

Tetracycline 500mg+ 4

Metronidazole 500mg 3-4

Proton pump inhibitor + 2

10-14 60-84 Clarithromycin 500mg + 2

Amoxicillin 1gm 2

Bismuth subsalicylate 525mg + 4 14 168

Tetracycline 500mg + 4

Metronidazole 500mg 3-4 Cheap option but efficacy reduced without

Proton pump inhibitor

Ranitidine bismuth citrate 400mg 2 7 42

Clarithromycin 500mg + 2 Cheap option but efficacy reduced without

Proton pump inhibitor Metronidazole 500mg 2

Hence an effective anti-ulcer herbal formulation—acting by multiple mechanisms of action

is required to heal the peptic ulcer as well as to effectively prevent their recurrence.

Concurrent use of NSAIDs especially in case of arthritic patients is one of the most common

aggressive factors. Therefore, we could develop a remedy which will not only target the ulcer

patients but also arthritic patients who has to take NSAIDs in their daily medication and always

increasing the risk of ulcer formation or the chances of recurrence. Therefore, we anticipate that

the standardization of Paederia foetida extract and its chemical modification to increase its

efficacy/potency, will impart a novel extract, which would be formulated.

16.3 Hypothesis

Ethno-medicinal plant Paederia foetida has common use among the various tribal communities

for the treatment of gastrointestinal problems in North–East region of India and other parts of

Asia. There are great chances to identify various plant products or extracts which might have

activity against peptic ulcer. While pure products have their own benefits, plant extracts have

synergistic effects of various molecules present in the extract. Our aim is to enrich them by bio-

guided fractionation method. Chemically engineered extracts of these active extracts will give us

access to various semi synthetic compounds and we can compare their synergistic effects with

those of natural extracts. It is quite possible that those molecules which were not stable in

natural extract could be available in chemically engineered extracts. The combined approach and

stepwise pharmacological study will give knowledge about potency of the chemically

engineered extracts as compared to the mother extract. This study will provide extracts which

could have activity against peptic ulcer.

16.4 Key questions:

1. How could we exploit the traditionally used Indian medicinal herbs to develop drug like

molecules / extracts?

8

2. Would creation of natural product like libraries from plant extract enhance the efficacy

of mother extract?

16.5 Current status of research and development in the subject

International status and National status

Peptic ulcer is a common disorder of gastrointestinal system and is well described in Ayurveda.

A variety of plant and their products have been reported to possess antiulcer activity 15

including

Ficus arnottiana (Moraceae), Glycyrrhiza glabra (Ligominosae), Asparagus racemosus

(Asparagaceae), Nerium indicum (Apocynaceae), Cassia nigrans (Mimosoideae), Swertia

chirata (Gentianaceae), Alstonia Scholaris (Apocynaceae), Azadirachta indica (Meliaceae) etc.

Plant contains several active principles which are responsible for antiulcer activity viz.

flavonoids, terpenoids, alkaloids, tannins. 16,17

Paederia foetida has diverse biological activity

viz. anti-diarrhoel,18

hepatoprotective,19

anti-arthritic,20

anti-tussive,21

anti-inflammatory,22

antioxidant,23

analgesic,24

anti cancer,25

etc. in different pre clinical studies due to the presence

of different secondary metabolites. Traditional uses of P. foetida are tabulated in Table 2.

Table 2 : Traditional uses of P. foetida

A number of phytoconstituents of P. foetida are reported in literature — Iridoid glycoside,

alkaloids, Fatty acids,27

Embelin & Friedelano,28

hentriacontane, hentriacontanol, ceryl

alcohol, sitosterol, stigmasterol, campesterol, ursolic acid and epifriedelinol29

.

16.6 The relevance and expected outcome of the proposed study

This study will explore the preparation and standardization technique, estimation of phyto-

Plant part

used

Location

(State/Country) Traditional uses

Leaf India Dyspepsia, flatulence, gastritis and enteritis, intestinal catarrh,

astringent, diarrhoea, stomach ache, abdominal distension and urinary

retention, relieve urinary calculi and dysuria,26a

flatulence and

rheumatism.

Assam, Allergy26d

, in gastralgia, post natal pain and bleeding26e

diarrhoea and

dysentery,26f-I

as vegetables26f

abdominal pain26j

Tripura As vegetable, diuretic, in diarrhoea, infection26k, l

Andhra Pradesh As vegetable26m

Arunachal

Pradesh,

Urinary disorder, kidney stone and digestive problem26n

also with rice as

vegetables in indigestion,26o

in gastric trouble, to clean stomach and

against stomach swelling and diarrhoea,26p

gastritis and loose motion26q

Sikkim and

Darjeeling

Anti diabetic26r

Meghalaya As anti dote for snake bite26s

China In indigestion, carminative, as antidote for insect bites 26a

Philippine Rheumatism, fever, allergy 26a

Malaysia

Indonesia

Swellings and as a poultice to treat nose ulcer, applied to abdomen in

cases of retention of urine; as diuretic and to dissolve vesicle calculi. 26b

Bangladesh Diarrhoea, to relieve distention and flatulence26c

Root India Emollient and carminative, treatment of colic, spasms, rheumatism,

emetic, gout26a

, piles, spleen ailment,26c

Asthma, seminal weakness26c

Bark Philippine Emetic26a

Fruit Philippine Toothache26a

9

constituents as markers, molecular characterization of the plant metabolites to provide

standardized extract of Paederia foetida rich in bio active principles. The goal of this study is to

provide bioactive extracts (chemically engineered or natural fractions) and a herbal formulation

containing most active fraction. Exploration of chemically engineered extracts would be a useful

tool for chemical library of natural products as area which would strengthen the record of Indian

medicinal plants.

16.7 Preliminary work done so far

I. An extensive field survey among the tribal communities residing in and near Agartala,

Tripura was done. A list of plants used by tribal communities was prepared and

commonly used plant by all communities was chosen (Paederia foetida) for current

studies.

II. Raw material was collected from the tribal area of Agartala and authenticated from

NISCAIR, New Delhi. (authentication Ref No. NISCAIR/RHMD/Consult/2010-

11/1442/40).

Figure 2 Antiulcer effect P. foetids

III. The leaves of Paederia foetida were dried at room temperature and extracted with

methanol using soxhlet apparatus (Yield of extraction was 36.21% w/w).

IV. Toxicological studies were done and dose tolerable found to be upto 2000 mg /kg body

weight. Biological activity for PUD was evaluated by three different models viz.

indomethacin-pylorus ligation induced ulcer, alcohol induced gastric ulcer and wate

immersion stress induced ulcer (WISIU) Ulcer protection represented graphically

(Figure. 2) for the dose of 100 and 200mg/kg body weight for each model respectively.

72.98

59.4562.5

78.5872.97

67

82.89 81.08

70

Indomethacin -pylorus ligation model

Alcohol induced gastric ulcer model

WISIU model

Anti Ulcer Effect of P. foetida

100 mg Extract 200 mg Extract Standard Drug

Figures represent %ase of ulcer protection

10

17. Specific Objectives:

Standardization of P. foetida extracts.

Bioassay guided fractionation leading to the identification and isolation of marker(s)

components

Chemical modification of most bioactive fractionate followed by its evaluation for

antiulcer activity

Comparison of activities of fractionates and their chemically engineered extracts

To develop a suitable herbal formulation from modified P. foetida extract for peptic

ulcer disease (PUD).

18. Work Plan:

Field Survey and collection of Plant Material: Field Survey has already been done. Adequate

amount of plant material was collected for initial work.

In-vitro method

Determination of Anti-Helicobacter pylori activities

1. Preparation of media: 24 g of Coloumbia agar was dissolved in 1.0 litre of re-distilled water

and autoclaved. To 950ml of this agar 50ml of sheep blood was mixed at 450C in addition, 28g

of Brain Heart Infusion broth was dissolved in 925 ml of re-distilled water and autoclaved. Then

75ml of heat-inactivated foetal calf serum was mixed to this broth at about 450C.

2. Organism and its growth: A Helicobacter pylori bacterial strain was cultured under micro-

aerobic conditions of 5% O2, 15% CO2 & 80% N2 at 370C on Columbia agar supplemented by

5% sheep blood.

3. Preparation of solutions :Sample solution (100mg & 200mg) were taken in 5ml of sterile N,

N-dimethyl formamide (DMF). Standard solution was prepared by dissolving 500 mg of

amoxicillin and Clarithromycin in 5ml of sterile DMF.

4. Procedure of anti-microbial testing methods

Turbidity method: Test sample, standard Sample & sterile DMF were added to 5ml of Brain

Heart Infusion broth supplemented by 7.5% heat activated calf serum separately. The tubes were

inoculated with H. Pylori strain (approximately 1.5X105 CFU/ml) and incubated under micro-

aerobic conditions (of 5% O2, 15% CO2 & 80% N2) at 370C for 72 hours. The turbidity of Brain

Heart Infusion broth under trials was read at 600 nm against the blank.

Inhibitory zone method : 50µl of Test sample, standard Sample & sterile DMF were added in the

holes on Colombia agar with 5% sheep blood plates separately. The plates were then inoculated

with the H. Pylori strains and incubated at 370C for 72 hours under micro –aerophilic conditions.

The plates were checked for the inhibitory zones around the wells in the agar media and

diameters of the zones of inhibition were measured. The results showed by the sample compared

with standard.

11

In-vivo method

Model 1 : Indomethacin-pylorus ligation-induced ulcer30

Rats/mice of either sex divided into five groups (n=5) will be taken. All the groups (except

group 1) will receive indomethacin (25mg/kg s.c.) once daily for three days. Group 1 will be

treated with vehicle (1ml distilled water/kg p.o.). Group 2 will be treated with indomethacin

(25mg/kg s.c.). Sample extract will be administered at standardized doses (obtained from dose

response studies) to group 3 & 4 respectively. Ranitidine (50 mg/kg p.o.) will be administered to

group 5. Methanolic extract or ranitidine will be administered to the respective groups 30 min

before each indomethacin treatment.

Surgical procedure30,31

On the fourth day after the treatment, surgical procedure will be performed. The rats/mice will

be fasted 24 hours before the starting of surgical procedure and provided free access to water

during this period. Each rat will be anaesthetized with Ketamine (45 mg/kg i.p.) and xylazine (5

mg/kg i.p.) and the abdomen will be exposed through a midline incision. The pylorus will be

located and ligated tightly with silk suture and stitched. Four hours after pylorus-ligation, the

animals will be sacrificed by cervical dislocation.

Determination of gastric volumes, pH and acid output30,32

The gastric contents from sacrificed rats/mice will be collected after 4 hours of pylorus ligation.

The gastric content will be centrifuged at 4000 rpm for 10 minutes. Volume, pH and acidity of

the supernatant of gastric contents will be measured precisely. The acid outputs will be

calculated by following equation:

Model 2 : Alcohol induced gastric ulcer33

The animals will be divided into five groups, each consisting of five rats/mice. In this case ulcer

will induce by administering 70% alcohol (10 ml/kg p.o.). All animals will be fasted for 36

hours before administration of alcohol. Group 1 will receive 1 ml distilled water/kg p.o. Group 2

will receive 70% alcohol (10 ml/kg, p.o. ) to induce gastric ulcer. Group 3 & 4 will receive

sample extract. Sucralfate will be administered to fifth group as reference standard drug at the

dose of 100 mg/kg, p.o. They will be kept in specially constructed cages to prevent coprophagia

during and after the experiment. The animals will be sacrificed by cervical dislocation and ulcer

score will be recorded.

Model 3 : Water immersion stress induced ulcer (WISIU) 34

Group 1 will receive 1 ml distilled water/kg p.o. Group 2 and 3 will receive sample extract at the

standardized doses (obtained from dose response studies). Group 4 will receive Lansoprazole (8

mg/kg). After treatment animals will be allowed to swim in a glass cylinder having ice cold

Acid out put (µEq/hr) = Acidity (mEq/L) X Vol. of gastric juice (mL/4hr)

12

water (4oC).

35 Animals will be sacrificed by cervical dislocation and gastric lesion in the

glandular region will be located in the gastric mucosa as elongated black red lines parallel to the

long axis of stomachs. The length (mm) of each lesion will be measured and lesion index will

calculated.

Plant Extract and Bio-guided Fractionation:

For preliminary studies, plant extract has been prepared using standard procedures.36

We would

check activity of crude extract using three pharmacological models to identify the best model for

study. The plant extracts will be then subjected to bioactivity guided fractionation to identify

active extract fractions. This suitable model will be used for screening of fractionates. We would

carryout chemical modification of active fractions and would test if they have better activity as

compared to mother fraction.

Preparation of chemically engineered extracts

Our approach to generate modified extract is based upon concept of chemically engineered

extracts (CEEs) preparation.1 Diversification of the components of natural extracts through

various functional group transformations could generate unique extracts. The reactions would be

selected on the basis of standard functional group tests.37

(Figure 3)

Following are the selected classes of reactions, which we will use once extracts are prepared and

fractionated.

Imine Formation Hydrazone Formation

Oxime Formation Disulfide Exchange

Sulphonylation Esterification & Hydrolysis

Oxidation-Reduction Reactions Bromination

Figure 3 Chemically Engineered Extracts

Formulation: The most active fractions will be combined and formulated. The dried extract will

be suspended in 0.3% carboxy methylcellulose solution.

13

18.1 Work Plan: Work plan is sketched in following scheme -1.

Scheme-1 Work Plan

Year 1: Plant Extract Preparation, Fractionation, Pharmacological Study

During first year of investigation, we will focus our studies on identification of antiulcer activity

in extract fractionation, their modification and method development of preparation of chemically

modified extracts. .

Year 2: Identification, Chemical Modification (Chemically Engineered Extract),

Pharmacological Study

Selection of plant and plant material

(leaf & stem) based on survey

100% pet

ether extract

100%

dichloromethane

extract

100% methanolic

extract

100% aqueous

extract

Bio guided fractionation (at least one in-vitro and two in-vivo method)

Identification and isolation of marker(s)

Chemical modification of most active fraction

Standardization of extract

Evaluation of activity and comparison with mother

extract/fractions

Formulation of suitable herbal preparation

14

During second year, we plan to execute our methods developed during Year I and prepare

chemically engineered extracts and their pharmacological activity.

Year 3: Pharmacological Studies and herbal formulation, Comparisons of activities,

Compilation of results and Final Report

This year we will focus our efforts in comparative studies, compilation of results and work will

be published in research journals.

19. Timelines:

Period of study Achievable targets

6 Months Procurement of Instruments, Chemicals, appointment of SRF

Preparation of different Extracts,

12 Months Phytochemical & chromatographic (TLC / HPTLC / HPLC) profiling,

pharmacological study bioassay guided fractionation at least one in-

vitro and two in-vivo model.

18 Months Identification of Most Active Fraction , Pharmacological Studies

identification and isolation of marker(s)

24 Months Preparation of Chemically Engineered Extract

30 Months Pharmacological Studies of chemically engineered extracts, at least one

in-vitro and two in-vivo model. Comparisons of activities

36 Months Formulation of suitable herbal preparation

Compilation of results and Final Report

20. Name and address of 5 experts in the field

Sr.No. Name Designation Address

1. Prof. Rajesh

Kumar Goel

Associate

Professor &

Head

Department of Pharmaceutical Sciences & Drug

Research, Punjabi University, Patiala,Punjab.

Pin -147002

2. Dr. Mohd. Ali Professor Department of Pharmacognosy and

Phytochemistry, Faculty of Pharmacy, Jamia

Hamdard, New Delhi 110062,

3. Dr. Biplab De Asst. Professor Department of Pharmaceutical chemistry, Regional

Institute of Pharmaceutical Science and

Technology, Central University Tripura 799006

4. Prof. S. M.

Sondhi

Professor Department of Chemistry, Indian Institute of

Technology Roorkee - 247667

5. Prof. Anil

Kumar

Associate

Professor

University Institute of Pharmaceutical Sciences

Punjab University Chandigarh

References:

15

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1997, 108, 599 15 a) Gadekar, R.; Singour, PK.; Chaurasiya, PK.; Pawar, RS.; Patil, UK. A potential of some medicinal plants as an

antiulcer agents pharmacog rev 2010,4,136-146 b) Sen, S.; Chakraborty, R.; De, D.; Mazumder, J. plants and phytochemical for peptic ulcer: An overview Pharmacog. rev 2009, 3, 270-279 c) Gulcin, I, Kufrevioglu, O. I.; Oktay, M.; Buyukokuroglu, M. E. Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle ( Urtica dioica L.) Journal of Ethnopharmacology 2004, 90, 205-215 d) Malairajan, P.; Gopalakrishnan, G.; Narasimhan, G.; Veni, KJ. K.; Kavimani, S. Anti-ulcer anctivity of crude alcoholic extract of Toona ciliata Roemer (heart wood) Journal of Ethnopharmcology 2007, 110, 348-351..

16 Mota, KSL; Dias, GEN; Pinto, MEF; Luiz-Ferreira, A.; Souza-Brito, ARM; Hiruma-Lima, CA.; Barbosa-Filho, JM; Batista L. M. Flavonoids with Gastroprotective Activity Molecules 2009, 14, 979-1012

17 Falcão H S.; Leite JA.; Barbosa-Filho JM.; Athayde-Filho PF; Chaves, M. C. O.; Moura, M. D.; Ferreira, A. L.; Almeida, A. B. A.; Souza-Brito, A. R. M.; Diniz, M. F. F. M.; Batista, L. M. Gastric and Duodenal Antiulcer Activity of Alkaloids: A Review Molecules 2008, 13, 3198-3223

18 Afroz S, Alamgir M, Khan MT, et al. Antidiarrhoeal activity of the ethanol extract of Paederia foetida Linn. (Rubiaceae), J Ethnopharmacol, 2006, 105, 125-130.

19 De S, Ravishankar B, Bhavsar GC, Evaluation of paederia foetida for Hepatoprotective and Anti-inflammatory Activities, Indian J Nat. Prod, 1993, 9, 7-10.

20 Chaturvedi GN, Singh RH, Experimental studies on the antiarthritic effect of certain indigenous durgs, Indian journal of medical research, 1965, 53, 71-80.

21 Nosalova G, Mokry J, Ather A, Khan MTH. Antitussive Activity of the Ethanolic Extract of Paederia foetida (Rubiaceae family) in Non-Anaesthetized Cats, Acta Vet. Brno, 2007, 76, 27-33.

22 De S, Ravishankar B, Bhavsar GC, Investigation of the anti-inflammatory effects of Paederia foetida, J of Ethnopharmacology, 1994, 43, 31-38.

23 Kumar V, Gogoi1 BJ, Meghvansi MK, et al. Determining the antioxidant activity of certain medicinal plants of sonitpur, (Assam), India using DPPH assay, Journal of Phytology, 2009, 1, 49–56.

24 Md Hossain M, Mohammad SA, Saha A, Md. Alimuzzaman, Antinociceptive activity of whole plant extracts of Paederia foetida, J Pharm Sci, 2006, 5, 67-69.

25 Costa-Lotufo LV, Khan MTH, Arjumand Ather, Studies of the anticancer potential of plants used in Bangladeshi folk medicine, Journal of Ethnopharmacology 2005, 99, 21–30.

16

26 a) Compendium of Medicinal Plants Used in Malaysia, 2002 Herbal Medicine Research Centre, Institute for

Medical Research, Kuala Lumpur, 2002,191-192. b) The Wealth of India, Raw material Vol VII. National Institute of Science Communication, CSIR, New Delhi, 2001, 210-211. c) http://www.stuartxchange.org/Kant d) Kalita D, Deb B, Folk medicines for some diseases prevalent in Lakhimpur district of Brahmaputra valley, Assam, Nat Prod Radiance, 5(4), 2006, 319-322. e) Purkayastha J, Nath SC, Biological activities of Ethnomedicinal claims of some plant species of Assam, Indian Journal of Traditional Knowledge, 2006, 5, 229-236. f) Barua U, Hore DK, Sarma R, Wild edible plants of Majuli island and Darraug districts of Assam, Indian Journal of Traditional Knowledge, 2007, 6, 191-194. g) Borah PK Gogoi P, Phukan AC, J Mahanta, Traditional medicine in the treatment of gastrointestinal diseases in upper Assam, Indian Journal of Traditional Knowledge, 2006, 5, 510-512. h) Basumatary SK, Ahmed M, Deka SP, Some medicinal plant leaves used by Boro (Tribal ) people of Goalpara district, Assam, Nat prod radiance, 2004, 3, 88-90. i) Kar A, Borthakur, Medicinal plants used against dysentery, diarrhea and cholera by the tribes of erstwhile Kameng district of Arunachal Pradesh, Nat prod radiance, 2008, 7, 176-181. j) Kalita D, Phukan B, Some Ethnomedicine used by the tai Ahom of Dibrugarh district, Assam India, Indian Journal of Natural Prod and Resources, 2010, 1, 507-511. k) Paushali Das, Wild tribal plants of Tripura tribes, 1

st ed, Tripura Tribal

Cultural Research Institute & Museum, Govt of Tripura, 1997,57-58. l) Chanda S, De B, Tiwari RK. Traditional and ethnobotanical investigation of some edible plants among th etribs of Tripura, India. In: Choudhury MD, Sharma GD, Choudhury S, Talukdar AD, Status and conservation of bio-diversity in North East India, 1

st ed.

Delhi Swastik publications, New Delhi, 2011, 118-124. m) Reddy KN, Pattanaik C, CS Reddy, Raju VS, Traditional knowledge on wild food plants in Andhra Pradesh, Indian Journal of Traditional Knowledge, 2007, 6, 223-229. n) Sarmah TR, Adhikari D, Majumdar M, Arunachalan A. Raditional medicobotany of chakma community residing in the Northwestern periphery of Namdapha National park in Arunachal Pradesh, Indian Journal of Traditional Knowledge, 2008, 7, 587-593. o) Kagyung R, Gajurel PR, Rethy P, Singh B, Ethnomedicinal plants used for gastrointestinal disease by Adi tribes of Dehang-Debang Biosphere Reserve in Arunachal Pradesh, Indian Journal of Traditional Knowledge, 2010, 9, 496-501. p) Srivastava RC, Singh RK, Apatani community, Mukherjee TK, Indigenous biodiversity of Apatani plateau: Learning on biocultural knowledge of Apatani tribe of Arunachal Pradesh for sustainable live hoods. Indian Journal of Traditional Knowledge, 2010, 9, 432-442. q) Sumpam Tangjang, Nima D Namsa, Chocha Aran, Anggu Litin, An ethnobotanical survey of medicinal plants in the Eastern Himalayas zone of Arunachal pradesh, India. J Ethnopharmacology, 2011, 134, 18-25. r) Chhetri DR, Parajuli P, Subba GC, Antidiabetic plants used by Sikkim and Darjeeling Himalayan tribes, India, Journal of Ethnopharmacology, 2005, 99, 199–202. s) Hynniewta SR, Kumar Y, Herbal remedies among the Khasi traditional healers and village folks in Meghalaya, Indian journal of traditional knowledge, 2008, 7, 581-586.

27 Shukla YN, Lloyd HA, Morton JF, Kapadia GJ. Iridoid glycosides and other constituents of Paederia foetida. Phytochem 1976; 15, 1989-1990.

28 Laurence L. Goodman and Gilman's The Pharmacological basis of Therapeutics. 9th Edition. UAS: Mc Graw-Hill companies Inc.; 1998

29 Ahmad MU, Islam MR, Huo E, Khan MW, Gupta S. Chemical constituents from Paederia foetida leaves. J Bangladesh Acad Sci 1991; 15, 19-22.

30 Yoshikawa, TNaito, YNakamura, SKaneko, T1993. Effect of Rabamipide on lipid peroxidation and gastric mucosal injury induced by indomethacin. Arzneim-Forsch/Drug Res. 43, 1327-1330.

31 Goel, R.KChakrabarti, ASanyal, A.K1985.The effect of biological variables on the anti ulcerogenic effect of vegetable plantain banana. Planta Medica 2, 85-8.

32 Kikuko AK, Shinichi Y, Hiroshi and O Susumu 1996. Effects of the nova histamine H2 receptor antagonist (±)-(E)-1-[2-hydrpxy-2-(4-hydroxyphenyl)ethyl]-3-[2-[[[5-(methyl amino) methyl-2-furyl] methyl]thio] ethyl]-2-(methylsulfonyl) guanidine on gastric secretion and gastroduodenal ulcers in rats. Arzneim-Forsch/Drug Res; 46:117-85.

33 Aguna, C.NUkwe, C.,1997. Gastrointestinal activities of Sterculia tragacantha leaf extract. Fitoterapia 68, 127-131.

34 Tanaka, TMorioka, YGebert, U.,1993. Effect of novel xanthene derivative on experimental ulcer in rats. Arzneim-Forsch/Drug Res. 43, 558-562.

35 Bhattacharya, S.KBhattacharya, D1982. Effect of restraint stress on rat brain serotonin. Journal of Bioscience 4, 267-274.

36 Houghton, P.JRaman, A1998. Laboratory handbook for the fractionation of natural extracts. Chapman and Hall, London.

37 Furniss, B.S. 1989. Vogel's Textbook of Practical Organic Chemistry, 5th

edn, Pearson Education India, New Delhi

17

PART IV: BUDGET PARTICULARS

Budget (In Rupees)

A. Non-Recurring (e.g. equipments, accessories, etc.):

S. No. Item Year 1

(in lakhs

Year 2

(in lakhs

Year 3

(in lakhs

Total (in

lakhs

1 FT IR SYSTEM 15 0 0 15.0

Sub-Total (A) 15 Lac

B. Recurring

B.1 Manpower

S.No. PositionNo. Consolidated

Emolument

Year 1

(in

lakhs)

Year 2

(in

lakhs)

Year 3

(in

lakhs)

Total

(in lakhs)

1. SRF - one Rs. 18,000 +

HRA 2.376 2.376 2.376 7.128

Sub-Total (B.1) = 7.128

B.2 Consumables

S. No. Item Year 1 Year 2 Year 3 Total (in

lakhs)

1.

Consumables for isolation,

purification and chemical

modification – various adsorbants,

solvents, HPLC Grade solvents,

Reagents and chemicals for

derivatization, modification of

extracts. Various metal Catalysts.

Other everyday use chemicals and

reagents for running Columns, TLC

and Reactions isolation and

purification reagents, glassware,

plasticware, UV-Cabinet etc.

3.0 3.0 2.0 8.0

2. Animal Studies 1.5 1.5 1 3.5

3.

NMR , IR and HPLC, LC-MS

analysis (approx @2000/- Per

sample) approx 100 samples for 3

years

2.0 2.0 2.0 6

Sub-Total (B.2) = Rs. 17.5 lakhs

18

Other items Consolidated Emolument Year 1 Year 2 Year 3 Total (in

lakhs)

B.3

Travel

Travel (Only inland travel) 0.5 0.5 0.5 1.5

B.4

Contingency

Other costs/Contingency

costs

2.0 2.0 2.0 6.0

B.5 Overhead

(If applicable)

Overhead As applicable

Sub-total of B (B.1+B.2+B.3+B.4): 7.128 + 17.5 + 1.5 +6.0= 32.128 lakhs

Grand Total (A + B): = 47.128 lakhs + Overheads As applicable

Part V: EXISTING FACILITIES

Resources and additional information

1. Laboratory: Chemistry Labs, Biochemistry Labs, Molecular Biology Labs, Tissue Culture

Labs

a. Manpower: Sr. Lab Technician (2); Lab Technician (3), Clerk (1), Peon (2).

b. Other resources: Animal House, glass house.

c. Equipments:

S.

No.

Name of equipment/accessories Make Funding

agency

Year of

procuremen

t

2. UV-Visible Spectrophotometer Elico JUIT 2003

3. Thermal Cyclers Applied Biosystems JUIT 2003

4. Vertical Gel Electrophoresis

System

Bangalore Genei JUIT 2003

5. SubmarineDNA Electrophoresis Bangalore Genei JUIT 2003

6. Low Temp Freezer (-20ºC) Vestafrost JUIT 2003

7. Ice Flaking Machine Manitowoc JUIT 2003

8. Water Purification System Millipore JUIT 2003

9. Electronic Analytical Balance Citizen JUIT 2003

10. pH Meter Elico JUIT 2003

11. Laminar Air Flow S M International JUIT 2003

12. High Speed Centrifuge Beckman JUIT 2003

13. Table Top Shaker Kuhner JUIT 2003

14. B.O.D. Incubators Hicon JUIT 2003

15. UV Transilluminator Bangalore Genei JUIT 2003

16. Plant Tiisue Culture Chamber Saveer JUIT 2003

17. Magnetic Stirrers Remi JUIT 2003

18. Incubator Shaker Kuhner JUIT 2003

19. Autoclaves Hicon JUIT 2003

20. Digital Camera Olympus JUIT 2003

21. Incubator (water bath) GFL (German) JUIT 2003

22. Clinical Centrifuges Olympus JUIT 2003

19

23. Walk-in-cold room Blue-Star JUIT 2003

24. Spectronic 20D+ Thermospectronic JUIT 2003

25. Electronic digital analytical

balance

Analytica JUIT 2004

26. Gel Doc BioRad JUIT 2005

27. LP system BioRad JUIT 2005

28. Low Temp Freezer (-80oC) New Buns Wick JUIT 2006

29. Freeze Dryer New Buns Wick JUIT 2006

30. HPLC System Waters JUIT 2006

31. Gas Chromatography Agilent Tech. JUIT 2006

32. Gene Pulser Xcell Total System BioRad JUIT 2007

33. Sequi-Gen GT Sequencing Cell BioRad JUIT 2007

34. CHEF-DR III Variable Angle

System

BioRad JUIT 2007

35. DNA Fluorometer BioRad JUIT 2007

36. Sonicator BioRad JUIT 2007

37. Phase Contrast Microscope Nikon JUIT 2007

38. In situ Setrilizable Fermentor 10L New Burns Wick JUIT 2007

39. CO2 Incubator New Buns Wick JUIT 2007

40. Chef DRIII Chiller Bio Rad JUIT 15/1/2008

41. Laboratory Spray drier Labultima/Mumbai JUIT 17/7/2008

42. Plant growth chamber Vista Bio-Cell Pvt.

Ltd.

JUIT 2/8/2008

43. Ultra Low Temp. Freezer (-80o C) New Brunswick

Scientific

JUIT 31/8/2008

44. Gel Doc System Alpha Ino Tech JUIT 30/9/2008

45. Incubator with Shaker

(MICROPROCESSOR)

New Brunswick

Scientific

JUIT 30/9/2008

46. PCR Machine 2 Nos Applied Biosystems JUIT 10/3/2008

47. Autoclave Mod. MLS3781 Sanyo SCIMED Asia Pvt.

Ltd.

JUIT 7/10/2008

48. Autoclave Mod. MLS3781 Sanyo SCIMED Asia Pvt.

Ltd.

JUIT 7/10/2008

49. 2 D PAGE system Bio Rad JUIT 31/12/2008

50. Atomic Absorption

Spectrophotometer

Perkin Elmer JUIT Jan. 2009

51. Centrifuge Allegrax Beckman Coulter JUIT 26/9/2009

52. RT PCR Biorad JUIT 5/11/2010

53. Millipore water Purification

System

Millipore JUIT 9/12/2010

54. Deep Freezer -80 D Newbrunswick JUIT 3/1/2011

55. Prep HPLC Waters DBT Oct 2011

20

PART VI: DECLARATION/CERTIFICATION

It is certified that

a) the research work proposed in the scheme/project does not in any way duplicate the work

already done or being carried out elsewhere on the subject.

b) the same project has not been submitted to any other agency/agencies for financial support.

c) the emoluments for the manpower proposed are those admissible to persons of

corresponding status employed in the institute/university or as per the Ministry of Science

& Technology guidelines (Annexure-III).

d) necessary provision for the scheme/project will be made in the Institute/University/State

budget in anticipation of the sanction of the scheme/project.

e) if the project involves the utilisation of genetically engineered organism, it is agreed that

we will ensure that an application will be submitted through our Institutional Biosafety

Committee and we will declare that while conducting experiments, the Biosafety

Guidelines of the Department of Biotechnology would be followed in toto.

f) if the project involves field trials/experiments/exchange of specimens, etc. we will ensure

that ethical clearances would be taken from concerned ethical Committees/Competent

authorities and the same would be conveyed to the Department of Biotechnology before

implementing the project.

g) it is agreed that any research outcome or intellectual property right(s) on the invention(s)

arising out of the project shall be taken in accordance with the instructions issued with the

approval of the Ministry of Finance, Department of Expenditure, as contained in

Annexure-V.

h) we agree to accept the terms and conditions as enclosed in Annexure-IV. The same is

enclosed.

i) the institute/university agrees that the equipment, other basic facilities and such other

administrative facilities as per terms and conditions of the grant will be extended to

investigator(s) throughout the duration of the project.

j) the Institute assumes to undertake the financial and other management responsibilities of

the project.

Signature of Project Coordinator Signature of Executive Authority

of Institute/University with seal

Date : December 14, 2011 Date : December 14, 2011

Signature of Principal

Investigator

Signature of Co-

Investigator

Signature of Co-

Investigator

Date Date Date

21

PART VII: PROFORMA FOR BIOGRAPHICAL SKETCH OF INVESTIGATORS

Provide the following information for the key personnel in the order listed on PART II.

Follow this format for each person. DO NOT EXCEED THREE PAGES

Name : Kuldeep Singh

Designation : Sr. Lecturer

Department/Institute/University : Department of Biotechnology , Bioinformatics &

Pharmaceutical Sciences, Jaypee University of

Information Technology

Date of Birth : 01 November 1978 Sex (M/F): M

Telephone: 01792-239391 FAX: 01792-245362

Education (Post-Graduation onwards & Professional Career)

S. No. Institution Place Degree Awarded Year Field of Study

I. IIT Roorkee M.Sc 2001 Chemical Sciences

II. IIT Bombay Ph.D 2007 Chemical Sciences

A. Position and Honors

Position and Employment (Starting with the most recent employment)

S. No. Institution /Place Position From

(Date)

To (date)

1. Jaypee University of


Waknaghat

Senior Lecturer July, 2010 Continuing

2. Jaypee Institute of Information

Technology Noida

Senior Lecturer November,

2009

June, 2010

3. Sai Adventium Pharma Ltd,

Pune

Research Scientist Feb 2009 Nov 2009

4. IIT Bombay Research Associate 01.11.2008 31.01.2009

5 University of Strasbourg ,

France

Postdoctoral

Scientist

01.11 2007 31.10.2008

6 IIT Bombay Research Associate 14.06.2007 25.10.2007

Honors/Awards

2007-2008 Postdoctoral Fellowship by University of Strasbourg, France

2003-2006 CSIR Senior Research Fellowship

22

2001-2003 CSIR Junior Research Fellowship

2001 Qualified Graduate Aptitude Test in Engineering (GATE)- Percentile 95.18

2000 Qualified Joint CSIR-UGC Test for J.R.F and Eligibility for Lectureship (NET)

1999-2001 Fee ship during M.Sc. awarded by I.I.T. Roorkee for position in Merit list.

1997 Selected for Award of ICAR National Talent Scholarship in Agricultural

Sciences.

Professional Experience and Training relevant to the Project

Applicant has training in organic chemistry. He will take care of chemistry part of the project.

B. Publications Research Papers: 05

I. Kotha, S., Bansal, D.; Singh, K; and Banerjee, S.; Synthesis of a new fluorescent

macrocyclic [alpha]-amino acid derivative via tandem cross-enyne/ring-closing

metathesis cascade catalyzed by ruthenium based catalysts, Journal of Organometallic

Chemistry, vol 696, pp. 1856-1860 , 2011.[ Indexed in SCOPUS, Impact factor: 2.205]

II. Kotha, S., Vittal, S., Singh, K., and Deodhar, K. D., ―Strategic utilization of catalytic

metathesis and photo-thermal metathesis in caged polycyclic frames", Tetrahedron

Letters, vol.51, pp. 2301-2304, 2010.[ Indexed in SCOPUS, Impact factor: 2.538]

III. Kotha, S., and Singh, K., ―Cross-enyne and ring-closing metathesis cascade: A building-

block approach suitable for diversity-oriented synthesis of densely functionalized

macroheterocycles with amino acid scaffolds", European Journal of Organic Chemistry,

pp. 5909-5916, 2007.[ Indexed in SCOPUS, Impact factor: 3.016]

IV. Kotha, S., and Singh, K., ―N-Alkylation of diethyl acetamidomalonate: Synthesis of

constrained amino acid derivatives by ring-closing metathesis reaction", Tetrahedron

Letters, vol.45, pp. 9607-9610, 2004.[ Indexed in SCOPUS, Impact factor: 2.538]

V. Thallapally, P. K., Jetti, R. K., Katz,A. K., Carrel,H. L., Singh, K., Lahiri, K., Kotha,

S.,Bose, R., and Desiraju, G., ―Polymorphism of 1, 3, 5-trinitrobenzene induced by

trisindane additive", Angewandte Chemie International Edition, vol.43, pp. 1149-1155,

2004.[ Indexed in SCOPUS, Impact factor: 12.73]

Place : JUIT WAKNAGHAT Signature of Investigator

Date : December 14, 2011

23




Name : Silpi Chanda

Designation : Assoc. Lecturer

Department/Institute/University: Department of Biotechnology, Bioinformatics &

Pharmaceutical Sciences, Jaypee University of Information Technology, Solan,Pin 173215

Date of Birth : 13/03/1979 Sex (M/F) F


Sl No. Institution

Place

Degree

Awarded

Year Field of Study

1 Oxford College of Pharmacy, Bangalore M. Pharm 2007 Pharmacognosy

A. Position and Honors


Sl No. Institution Place Position From To (date)

1 Jaypee University of Information

Technology, Wakhnaghat, HP

Assoc. Lecturer Jan 2011 Till date

2 Innovative College of Pharmacy,

Greater Noida, UP under Uttar

Pradesh Technical University.

Lecturer Nov 2009 Dec 2010

3 College of Pharmacy, IILM, Greater

Noida, UP under Uttar Pradesh

Technical University.

Lecturer Oct 2008 Nov2009

4 Nutan Education Trust B. Pharmacy

college, Godhra, Gujrat Technical

University

Lecturer Dec 2007 Oct 2008

5 Torrel Cosmetics –Sister concern of

Torrent pharmaceuticals, Ahmedabad,

Gujrat

F & D Executive Feb 2007 Dec 2007

24

Honors/Awards

Four (04) year state scholarship under the Govt. of Tripura through out the tenure of B.

Pharmacy


1. The applicant has been engaged in R&D projects on isolation and characterization of

phytoconstituents from Medicinal and Aromatic plants in Natural Remedies Pvt. Ltd.

Bangalore.

2. The applicant has experienced on various aspects of Chromatographic technique including

TLC, HPTLC and HPLC and method validation technique.

3. Drug testing at Central Drug Laboratory (CDL), Govt of Tripura, India.

B. Publications (Numbers only)

Books : None Research Papers: 07 Communication 02

List of publications:

S. Chanda, Shalini Kushwaha and Raj kumar Tiwari. 2011. Garlic as food, spice and medicine :

A prespective. Journal of Pharmacy Research. 4(6): 1857-1860

S. Chanda, Manish Bagga and Raj kumar Tiwari. 2011. Microneedles in transdermal drug

delivery : An unique painless option. International Journal of Pharmacy 2(4):72-78

B De, A. Chakraborty, T Majumder, S. Chanda and B.B. Goswami. 2011. Evaluation of

Spermicidal and Antimicrobial Activities of methanolic extract of Leucas aspera and Structural

elucidation of separated active component. Asian Journal of Chemistry, 23(2) : 826-828.

B De, A. Chakraborty, T Majumder, S. Chanda and B.B. Goswami. 2011. Evaluation of

Spermicidal and anticoagulant activities of methanolic extract of Mimosa pudica and Structural

elucidation of separated active component. Asian Journal of Chemistry. 23(2): 832-834.

R. K. Tiwari, S. Chanda, M. Deepak, B. Murli and A. Agarwal. 2010. HPLC method validation

for simultaneous estimation of Madecassoside, Asiaticoside and Asiatic acid in Centella

asiatica. Journal of Chemical and Pharmaceutical Research. 2(3) : 223-229.

S. Chanda & Padmaa M Parakh. 2008. Crateava nurval-Buch-a review In Prarmacog. Rev.

2(3):18-21.

De, S. Raha, S. Chanda, B. Debbarma 2007. Antibacterial evaluation of the extracts of edible

parts of fewplants used by tribal people of Tripura India. In the Journal of Pure &Applied

Microbiology- An International Research Journal of Microbiology. 1:65-68



25




Name : M. Udayabanu

Designation : Lecturer

Department/Institute/University : Department of Biotechnology, Bioinformatics &

Pharmaceutical Sciences, Jaypee University of


Date of Birth : 01 Sep’1977 Sex (M/F) M


Sl No. Institution

Place

Degree

Awarded

Year Field of Study

1. SGSITS, Indore M.Pharm 2002 Medicinal and

Pharmaceutical Chemistry

2. Dr.Ambedkar Center for

Biomedical Research,

Delhi University.

PhD. Thesis

Submitted

Biomedical Sciences

B. Position and Honors


Sl No. Institution / Place Position From (Date) To (date)

1 Jaypee University of Information

technology, Waknaghat

Lecturer 1.7.2010 Till date

2 Jaypee Institute of Information

Technology, Noida Lecturer Nov 2009 June 2010

3 Dr.B.R.Ambedkar center for

Biomedical research, Delhi

University

Teaching

Assistant August 2005 June 2006

4 RKGIT, Ghaziabad Lecturer Oct 2004 June 2005

5 RMSITS, Madhya Pradesh Lecturer Feb 2002 Sep 2004

26

Honors/Awards

a. Recipient of Junior research Scholarship from AICTE

b. Recipient of Senior Research Fellowship from ICMR

c. Member of Academic council for Post Graduate in S.G.S.I.T.S., Indore, INDIA

d. Ranked # 12 in national level Graduate Aptitude Test in Engineering exam with

99.61 percentile conducted by Indian Institute of Technology in 2000

e. Ranked as #1 in educational achievement at undergraduate level


Expertise in animal handling

Pharmacological screening of plant extract like Withania extract, Hypeicum extract,

Stringinging nettle extract

C. Publications (Total number of research articles published: 04)

List maximum of five recent publications relevant to the proposed area of work.

1. Udayabanu, M., Kumaran, D., Nair, R.U., Srinivas, P., Bhagat, N., Aneja, R., Katyal, A.

Nitric oxide associated with iNOS expression inhibits cetylcholinesterase activity and induces

memory impairment during acute hypobaric hypoxia‖ Brain Res, Vol. 1230, pp. 138-49, 2008.

2. Kumaran, D., Udayabanu, M., Kumar, M., Aneja, R., Katyal, A., ―Involvement of

angiotensin converting enzyme in cerebral hypoperfusion induced anterograde memory

impairment and cholinergic dysfunction in rats‖ Neuroscience, Vol.155, pp. 626-39, 2008.

3. Kumaran, D., Udayabanu, M., Nair, R.U., Aneja, R., Katyal, A., ―Benzamide protects

delayed neuronal death and behavioural impairment in a mouse model of global cerebral

ischemia‖ Behav Brain Res, Vol. 192, pp. 178-84, 2008.

4. Udayabanu, M., Kumaran, D., Katyal, A. Free chelatable zinc modulates the cholinergic

function during hypobaric hypoxia induced neuronal damage: An in-vivo study. Neuroscience.

(Accepted)

C. Research Support

Ongoing Research Projects

Sl No. Title of Project Funding Agency Amount Date of sanction and Duration

1 Formulation of an

antidepressant/stress

reliever drug based on

St John‘s Wort

DRDO, New

Delhi

15 Lakhs Nov 2008 for 3yrs



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