Specializing in Secondary Antibodies Conjugates · indicated on the packing list. All claims for missing items must be reported directly to Jackson ImmunoResearch within 5 days after

ImmunofluorescenceImmunohistochemistryImmunocytochemistry

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In Situ HybridizationElectron Microscopy

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ELISA

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LABORATORIES, INC.

Specializing in

Secondary Antibodies and Conjugates

2

Contact and Ordering Information

Contact Information:

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please see page 7, Step 7)8. Quantity

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Discounts: The following discounts are automatically given for each purchase order,unless otherwise agreed upon.5 %.................................$500 - $1,000 10 %...............................$1,001 - $1,500 15 %...............................$1,501 - $2,000 20 %...............................over $2,000

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3www.jacksonimmuno.com Telephone: 800-367-5296 Fax: 610-869-0171

Contents

Ordering email: [email protected] Technical email: [email protected]

Ordering Information.....................................................................inside front cover

Technical Information - Affinity-Purified Secondary Antibodies .....................4Selection and Location of Affinity-Purified Secondary Antibodies ..........................4Multiple Labeling (ML) Using Conjugated Secondary Antibodies ..........................7Probes Conjugated to Affinity-Purified Antibodies and to Other Proteins...............8

Fluorescent Dyes...........................................................................................8Alexa Fluor®.......................................................................................8-12Cyanine (CyTM2, Cy3, Cy5) ...............................................................8-12DyLight 405 ..........................................................................................10Aminomethylcoumarin Acetate (AMCA).................................................10Fluorescein Isothiocyanate (FITC/DTAF) ...............................................11Rhodamine RedTM-X (RRX) ...................................................................11

Biotin-SPTM (long spacer) ............................................................................13Enzymes......................................................................................................13

Whole IgG Affinity-Purified Secondary Antibodies ....................................14-35

F(ab')2 Fragment Affinity-Purified Secondary Antibodies .........................36-45

Monovalent Fab Fragment Affinity-Purified Secondary Antibodies for Blocking and for Double Labeling Primary Antibodies from the Same Host Species.......................................................................................46-52

Secondary Antibodies for Flow Cytometry ..................................................53-58

IgG Controls and Streptavidin Conjugates for Flow Cytometry.....................58

Anti-Mouse IgG Subclass Specific Antibodies ................................................60

Anti-IgG, Light Chain Specific Antibodies for Western Blotting after Immunoprecipitation (IP) ...........................................................................62

Alexa Fluor® 680 and Alexa Fluor® 790 Conjugates for High Sensitivity Western Blots ...........................................................................64

Cy2, Cy3, and Cy5 Conjugates for Permanent Mounting ................................68

Streptavidin ...........................................................................................................71

ImmunoGold Reagents........................................................................................72LM Grade 4 nm Colloidal Gold - Antibody Complexes........................................73EM Grade 6 nm, 12 nm, and 18 nm Colloidal Gold - Antibody Complexes .........74

Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes .................75

Solid-Phase Immunoadsorbent Gels ................................................................75

Miscellaneous Antibodies ..................................................................................76Purified IgG Fraction Monoclonal Mouse Anti-Digoxin ........................................76Purified IgG Fraction Monoclonal Mouse Anti-Biotin...........................................76Purified IgG Fraction Monoclonal Mouse Anti-Fluorescein..................................76Affinity-Purified Anti-Horseradish Peroxidase ......................................................76

Normal Serums and Gamma Globulins.............................................................78

Bovine Serum Albumin (IgG-Free, Protease-Free)..........................................79

Purified Proteins from Normal Serums........................................................80-89

Antisera to Immunoglobulins, Whole Serums, and Enzymes........................90

International Distributors....................................................................................91

Trademarks and Licenses .................................................................................. 95

4

Technical Information - Affinity-Purified Secondary Antibodies

Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatographyusing antigens coupled to agarose beads. A proprietary elution process is used todissociate antibodies from the antigen. Unconjugated affinity-purified antibodies aresupplied sterile-filtered in phosphate buffer without stabilizers or preservatives. Conjugatedaffinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodiumazide, with the exception of horseradish peroxidase conjugates, which do not contain apreservative. Alkaline phosphatase conjugates are freeze-dried in Tris buffer withstabilizers and sodium azide.

Selection and Location of Affinity-Purified Secondary Antibodies

Step 1. Affinity-purified secondary antibodies are offered in three different forms.Select from Whole IgG (pages 14-35), F(ab')2 fragment (pages 36-45), or Fab fragment(pages 46-52) antibodies.

Whole IgG (pages 14-35) antibodies are isolated as intact molecules from antisera byimmunoaffinity chromatography. They have an Fc portion and two antigen binding Fabportions joined together by disulfide bonds (Figure 1), and therefore they are divalent. Theaverage molecular weight is reported to be about 160 kDa. The whole IgG form ofantibodies is suitable for the majority of immunodetection procedures and is the most costeffective.

F(ab')2 fragment (pages 36-45) antibodies are generated by pepsin digestion of wholeIgG antibodies to remove most of the Fc region while leaving intact some of the hingeregion. F(ab')2 fragments have two antigen-binding Fab portions linked together bydisulfide bonds, and therefore they are divalent. The average molecular weight is about110 kDa. They are used for specific applications, such as to avoid binding of secondaryantibodies to live cells with Fc receptors or to Protein A or Protein G.

However, binding of primary antibodies to Fc receptors also may occur if they are wholeIgG antibodies, creating background regardless of the form of the secondary antibody.To block whole IgG primary and secondary antibodies from binding to Fc receptors,

incubate cells in buffer containing 5% normal serum from the host species of the labeledsecondary antibody. To prevent capping, endocytosis, and regeneration of Fc receptorson living cells, incubate at 4°C in buffer containing 5% normal serum with sodium azideadded to inhibit metabolism.

Caution: Never block with normal serum or IgG from the host species of the primary antibody.If immunoglobulins in normal serum bind to the specimen of interest, they will be recognizedby the labeled secondary antibody, resulting in higher background.

Bovine serum albumin (BSA) and dry milk, both commonly used for blocking, may containbovine IgG. With the exception of Bovine Anti-Goat IgG, many secondary antibodies such asAnti-Bovine, Anti-Goat, and Anti-Sheep will react strongly with bovine IgG. Therefore, use ofBSA or dry milk for blocking or diluting these antibodies may significantly increase backgroundand/or reduce antibody titer. For blocking, use normal serum (5% v/v) from the host speciesof the labeled secondary antibody.

LH

LH

lgGLH

LH

F(ab’)2L

HFab

fragments

pepsin

digestion

LH

LH

lgG

papain

digestion

over

digestion+

+L

H

H H

Fab Fc

Figure 1. Schematic representation of IgG fragments generated by enzymatic digestions.

Figure 1.




Fab fragment (pages 46-52) antibodies are generated by papain digestion of whole IgGantibodies to remove the entire Fc portion, including the hinge region (Figure 1). Theseantibodies are monovalent, containing only a single antigen binding site. The molecularweight of Fab fragments is about 50 kDa. They can be used to block endogenousimmunoglobulins on cells, tissues, or other surfaces, and to block the exposedimmunoglobulins in multiple labeling experiments using primary antibodies from the samespecies.

In contrast, divalent (whole IgG or F(ab')2 fragment) antibodies should not be used forblocking since they have two binding sites. After blocking, some of the binding sites wouldbe available to capture the primary antibody introduced in a subsequent step, resulting inhigher background and/or coincidental labeling.

Step 2. Select the secondary antibody.The antibodies are listed alphabetically according to the host species of the primary antibody.For example, if the primary antibody is made in mouse, go to the "Anti-Mouse" section.

Note: Both anti-Syrian and anti-Armenian hamster secondary antibodies are listed under"Anti-Hamster". It is important to know in which strain of hamster the primary antibodywas produced since cross-reaction between the strains is not complete.

Step 3. Select the host species of the secondary antibody.Selection of the host species for a secondary antibody involves many considerations,including but not limited to: 1) Antibodies from some host species may not be adsorbedagainst cross-reacting species of interest. Choose a host species with the requiredadsorptions. 2) Host species compatibility. Some host species may not be compatiblewith other species in multiple-labeling experiments. In general, all secondary antibodiesfor multiple labeling should come from the same host species. 3) Binding to Protein Aand Protein G. Rabbit antibodies bind well to Protein A and Protein G, but goat anddonkey antibodies bind better to Protein G. 4) Personal preference or experience. In ourexperience there appears to be no host species-specific difference in the quality ofsecondary antibodies.

Step 4. Select the secondary antibody specificity under "Antibody Description".The following explanations of terms may assist in selecting the most appropriate antibodyspecificity.

Note: Immunoglobulins from different species share similar structures, with similaritiesbeing related to closeness in phylogeny. Antibodies against immunoglobulins from onespecies may cross-react with a number of other species unless they have been specificallyadsorbed against the cross-reacting species. Antibodies that have been adsorbed againstother species will contain "(min X...Sr Prot)" in the antibody description.

Anti-IgG (H+L)These antibodies react with both the heavy and light chains of the IgG molecule, i.e. withboth the Fc and F(ab')2 / Fab portions of IgG (Figure 1). Anti-IgG (H+L) antibodies alsoreact with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since theyall share the same light chains (either kappa or lambda). Anti-IgG (H+L) antibodies havebroader epitope recognition than anti-fragment specific antibodies. They are suggestedfor all general immunodetection procedures.

Anti-IgG, Fc/Fcγ fragment specificThese antibodies react with the Fc portion of the IgG heavy chain. They have been testedby ELISA and/or adsorbed against Fab fragments. In some cases, they are additionallytested and/or adsorbed to minimize cross-reactivity to IgM and/or IgA. In such cases(anti-human, anti-mouse, and anti-rat), they are labeled "Anti-IgG, Fcγ".

Caution: Anti-IgG, Fcγ fragment specific antibodies may not react equally with all monoclonalprimary antibodies. For an anti-mouse IgG, Fcγ fragment specific antibody with balancedreactivity to four subclasses of IgG, select goat anti-mouse IgG (subclasses 1+2a+2b+3), Fcγfragment specific (min X Hu, Bov, Rb Sr Prot).

Anti-Mouse IgG, Fcγ subclass specificThese antibodies react with the Fc portion of the heavy chain of individual subclasses ofmouse IgG. They have been tested by ELISA and/or adsorbed to minimize cross-reactivity

6


to other subclasses, Fab fragments, IgM, and a few other species of IgG. Anti-MouseIgG, Fcγ subclass specific antibodies react with individual subclasses of mouse IgG. Theyare intended for distinguishing between different subclasses of mouse IgG primaryantibodies in multiple labeling experiments, or for IgG subclass determination.

Anti-IgG, F(ab')2 fragment specificThese antibodies react with the F(ab')2 / Fab portion of IgG. They have been tested byELISA and/or adsorbed against Fc fragments. They are not specific for IgG since theyreact with light chains, and therefore also react with other immunoglobulin classes (IgA,IgM, IgD, and IgE) and subclasses sharing the same light chains.

(min X ... Sr Prot)Secondary antibodies against one species may cross-react with other species unless theyhave been specifically adsorbed against the other species. Antibodies with "(min X ... Sr Prot)"in the description have been tested and/or adsorbed against IgG and/or serum proteins ofthose species indicated in the parentheses. They are recommended when the presence ofimmunoglobulins from other species may lead to interfering cross-reactivities. However,caution should be exercised when considering antibodies that have been adsorbed againstclosely related species, since they have greatly reduced epitope recognition and mayrecognize some monoclonals poorly. For example, only use anti-mouse IgG adsorbed againstrat IgG to detect a mouse primary antibody in rat tissue which contains endogenous ratimmunoglobulins, or in a multiple labeling application which includes a rat primary antibody.Use anti-mouse IgG not adsorbed against rat IgG to detect a mouse primary antibody in theabsence of rat immunoglobulins. Two other examples of antibodies which have diminishedepitope recognition after adsorption with closely related species are Anti-Rat IgG (min X ... Mouse ... Sr Prot) and Anti-Armenian Hamster IgG (min X ... Mouse, Rat ... Sr Prot). Refer to "ML (Multiple Labeling)" for further information.

The following abbreviations are used in the parentheses:

ML (multiple labeling) Some antibodies are designated "ML" to emphasize theirusefulness in multiple labeling in addition to single labeling. For further information see: "Multiple Labeling (ML) Using Labeled Secondary Antibodies" (page 7).

Anti-Armenian Hamster IgG vs. Anti-Syrian Hamster IgGMost hamster monoclonal antibodies are derived from Armenian hamster spleen cell-mouse myeloma hybridomas. The IgG produced by these hybridomas is Armenian (notSyrian) hamster IgG. Most commercially available polyclonal anti-hamster IgG antibodiesare anti-Syrian hamster IgG, which are not as effective as anti-Armenian hamster IgG indetecting Armenian hamster IgG monoclonal antibodies.

Caution: Anti-Armenian Hamster IgG (H+L) (min X Bov, Hu, Ms, Rb, Rat Sr Prot) may notrecognize all Armenian hamster monoclonal antibodies, since it has been adsorbed againstclosely related species (in bold). Therefore, it is better to use an antibody adsorbed againstfewer species, such as Anti-Armenian Hamster IgG (H+L) (min X Bov Sr Prot), except in thosecases where Armenian hamster monoclonals need to be detected in the presence of mouseand/or rat immunoglobulins.

Step 5. Select the desired probe from those listed at the top of the product tables.For technical information about probes, see "Technical Information on Probes Conjugatedto Affinity-Purified Antibodies and to Other Proteins" (page 8).

min X = minimal cross-reactionBov = BovineCk = ChickenGt = GoatGP = Guinea Pig

Ar Hms = Armenian HamsterSy Hms = Syrian HamsterHrs = HorseHu = HumanMs = Mouse

Rb = RabbitShp = SheepSw = SwineSr = SerumProt = Protein




Step 6. Find the size and code number of the selected antibody by following the rowuntil it intersects the column of the desired probe.The top numbers in each cell refer to the unit size, and the bottom nine-digit number isthe catalog code number.

Step 7. Complete product description for ordering purposes.For a complete description of the product, please use the following format to avoid mistakeswhen placing an order. For example, a product with the code number 115-096-072 shouldbe described as below:

Multiple Labeling (ML) Using Labeled Secondary Antibodies

Selection of antibodies for simultaneous detection of more than one antigen depends onat least two important criteria: 1. Availability of secondary antibodies that do not recognize (a) one another (are derivedfrom the same host species), (b) other primary antibodies used in the assay system, (c) immunoglobulins from other species present in the assay system, or (d) endogenousimmunoglobulins present in the tissues or cells under investigation. 2. Use of probes (enzyme-reaction products, fluorophores, or electron-dense particles)that are well resolved.

The affinity-purified antibodies marked "ML" (multiple labeling) have been specificallyprepared to meet these criteria. One of many possible multiple-labeling protocols usingthese reagents is shown in the following example.

Note: Wash thoroughly after each step, including after blocking at step 1. With heavy or persistent background furtherblocking may be required at Steps 4 and 7. Do not dilute any antibody with normal serum or mix antibodies together tosave time, which may result in immune complex formation which could increase background.

In this example, the secondary antibodies used in Steps 3, 6 and 9 do not recognize each other since they are all made indonkey. They have been solid-phase adsorbed so that they do not recognize the other primary antibodies used in Steps2, 5, and 8. Also, they do not react with endogenous mouse Ig, which may be present in the mouse tissue.

For a review of multi-color immunofluorescence labeling with confocal microscopy see Brelje, Wessendorf, andSorenson, "Multi-color laser scanning confocal immunofluorescence microscopy: Practical application and limitations." InCell Biological Applications of Confocal Microscopy (Methods in Cell Biology. vol. 38). Ed. B. Matsumoto. Orlando, FL:Academic Press, Inc. 1993, pp. 98-181.

Mouse Tissue Antigen A Mouse Tissue Antigen B Mouse Tissue Antigen C

Step 1: 5% N. Donkey Serum toblock

Step 2: Goat Anti-Antigen A

Step 3: Probe l-conjugatedDonkey Anti-Goat IgG(H+L) (min X Ck, GP, Sy Hms,Hrs, Hu, Ms, Rb, Rat Sr Prot)

Step 4: 5% N. Donkey Serum toblock (if needed)

Step 5: Rabbit Anti-Antigen B

Step 6: Probe ll-conjugatedDonkey Anti-Rabbit IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms,Hrs, Hu, Ms, Rat, Shp Sr Prot)

Step 7: 5% N. Donkey Serum to block (if needed)

Step 8: Rat Anti-Antigen C

Step 9: Probe lll-conjugatedDonkey Anti-Rat IgG(H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)

FITC-conjugated AffiniPure F(ab')2 fragment Goat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot)

A B C D E F G

A. Description of the probe, if it is conjugated. If unconjugated, nothing is required here.B. AffiniPure is our trade name for antibodies which have been isolated from antisera

by immunoaffinity chromatography using antigens coupled to agarose beads.C. Form of the antibody - whole IgG, F(ab')2 fragment, or Fab fragment antibody.D. Name of the host species of the secondary antibody.E. Name of the species with which the antibody reacts.F. Description of the antibody specificity.G. List of species against which the antibody has been adsorbed to minimize cross-reactivity.

Key to the format of product descriptions eg: FITC-conjugated AffiniPure F(ab')2 fragmentGoat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs, Sr Prot)

Technical Information on Probes Conjugated to Affinity-PurifiedAntibodies and to Other Proteins.

Fluorescent Dyes (for Phycoerythrin, PerCP, and Allophycocyanin conjugates, see page 53)

Selection of fluorophores depends on:A. Instrument set-up. Examples include availability of light sources, filter sets, anddetection systems.

B. Degree of color separation desired for multiple labeling. For example, to achieve goodcolor separation from Alexa Fluor® 488, choose a shorter wavelength-emittingfluorophore such as DyLight 405 and/or longer wavelength-emitting fluorophores suchas Rhodamine Red-X, Alexa Fluor® 594, or Alexa Fluor® 647.

C. Sensitivity required. For example, Alexa Fluor® 488 is brighter than FITC.

The following fifteen fluorescent dye conjugates (Figure 2, Table 1) are currently availablefrom Jackson ImmunoResearch. They cover the most commonly used excitation sourcesand filter sets from blue to infrared emissions.

Alexa Fluor® Fluorescent Dyes Alexa Fluor® fluorescent dyes are widely recognized as superior fluorescent dyes availablefor conjugation. They are highly water soluble and remain fluorescent from pH 4 to pH 10. The detection level of any fluorophore-antibody conjugate depends on brightnessand photostability of the dye; antibody activity, specificity, and cross-reactivity; and theoptimal moles of dye per mole of antibody. These parameters have been researched foreach dye conjugate to optimize the level of antibody detection and minimize background.

Cyanine dyes (Cy2, Cy3, and Cy5)Among currently available fluorescent dyes, the cyanine dyes are better able to withstand theharsh dehydration and embedding conditions required for mounting sections in non-polar

plastic mounting media such as DPX and Permount™. The cyanine dyes are brighter in thenon-polar environment than in an aqueous medium, resulting in less acquisition time thanother dyes in the confocal microscope. The advantage of using plastic mounting media isbetter long-term storage for re-examination and retrieval of data at a later date. See pages68-70 for a list of cyanine dye conjugates.

8

Technical Information - Probes

Fluorophore Excitation Peak

400

350

492

493

492

550

many, 488

570

591

many, 650

651

650

many, 488

684

792

Emission Peak (nm)

421

450

510

519

520

570

580

590

614

660

667

670

675

702

803

Table 1. Approximate peak wavelengths of excitation and emission for all fluorophore-conjugated,affinity-purified secondary antibodies offered by Jackson ImmunoResearch in order by increasing peakof emission. Approximate values are given for purposes of comparing one fluorophore with another.Actual values may vary depending on the spectrofluorometer used in each laboratory.

DyLight 405

Aminomethylcoumarin, AMCA

Cyanine, Cy2

Alexa Fluor® 488

Fluorescein, FITC/DTAF

Indocarbocyanine, Cy3

R-Phycoerythrin R-PE

Rhodamine Red-X, RRX

Alexa Fluor® 594

Allophycocyanin APC

Alexa Fluor® 647

Indodicarbocyanine, Cy5

Peridinin-Chlorophyll-protein, PerCP

Alexa Fluor® 680

Alexa Fluor® 790




Figure 2. Excitation (left) and emission (right) spectra of allfluorophores offered by Jackson ImmunoResearch. Theconjugates in order of increasing wavelength offluorescence are DyLight 405 (violet), AMCA (dark blue),Cy2 (light blue), Alexa Fluor® 488 (blue-green), FITC (hidden behind Alexa Fluor® 488), Cy3 (yellow), R-PE (dark pink, dotted), Rhodamine Red-X (orange),Alexa Fluor® 594 (red), Allophycocyanin (pink, dotted), Cy5 (green), Alexa Fluor® 647 (purple-hidden behind Cy5),PerCP (lavender, dotted), Alexa Fluor® 680 (gray), andAlexa Fluor® 790 (black).

This figure illustrates the relative shape and position of eachfluorophore in the peak region of its excitation andfluorescence emission following conjugation to antibodies.Quantitative comparisons should not be made since peakheights have been normalized. All spectra were obtainedwith an M-Series spectrofluorometer system from PhotonTechnology International, Inc.

Exci

tatio

n

900Wavelength (nm)

8007006005004002000

300

Fluo

resc

ence

Wavelength (nm)850750650550350

0450

Figure 3. Excitation (left) and Emission (right) spectra ofAlexa Fluor®-conjugated affinity-purified secondaryantibodies, streptavidin, and purified proteins. The dyes areAlexa Fluor® 488 (blue), Alexa Fluor® 594 (green), Alexa Fluor® 647 (red), Alexa Fluor® 680 (pink), and Alexa Fluor® 790 (black).

This figure illustrates the relative shape and position of eachfluorophore conjugate in the peak region of its excitation andfluorescence emission. Quantitative comparisons should notbe made since peak heights have been normalized. Allspectra were obtained with an M-Series spectrofluorometersystem from Photon Technology International, Inc.

Exci

tatio

n

Wavelength (nm)850750650550350

0450

Fluo

resc

ence

Wavelength (nm)850800750700650600550450

0500

Figure 2.

Figure 3.

10


Fluorescent Dyes in Order of Increasing Emission WavelengthA brief description of the characteristics of fluorophores found in this catalog is foundbelow. The fluorophores are listed in order of increasing emission wavelength (Figure 2and Table 1). For Phycoerythrin, PerCP, and Allophycocyanin see page 53.

DyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm andfluoresce with a peak at about 421 nm (Figure 2 and Table 1). They are very bright andphotostable, but their optimal use is limited to confocal microscopes equipped with a405 nm laser and a 420 nm emission filter. Under these conditions, it is possible toperform effective 4-color imaging with good color separation, good photostability, and highsensitivity. The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, andAlexa Fluor® 647 provides for maximum color separation (Figure 5). Another 4-color dyecombination, which may be equally effective but has slightly less color separation, isDyLight 405, Alexa Fluor® 488, Cy3, and Alexa Fluor® 647. DyLight 405 is notrecommended for use in epifluorescence microscopes, nor is it recommended for

flow cytometry, because emission filters generally used in flow cytometers are not optimalfor DyLight 405. DyLight 405 conjugates are the best choice for blue-fluorescingsecondary antibodies in multi-color labeling protocols. See tables of Whole IgG Affinity-Purified Antibodies (pages 14-35), Streptavidin (page 71), and Purified Proteins fromNormal Serums (pages 80-89) for all DyLight 405 conjugates.

Aminomethylcoumarin Acetate (AMCA) conjugates absorb light maximally around 350 nmand fluoresce maximally around 450 nm (Figure 2 and Table 1). For fluorescencemicroscopy, AMCA can be excited with a mercury lamp and observed using a UV filter set.Since blue fluorescence is not well detected by the human eye, AMCA-conjugatedsecondary antibodies should be used only with the most abundant antigens in multiple-labelingexperiments. Ways of improving the visibility of AMCA include dark adapting the eyes, usingfluorite instead of glass objectives, avoiding mounting media that absorb UV light (such asplastic-based media), and capturing photographic images with blue-sensitive film or CCDcameras. AMCA fades rapidly in conventional epifluorescence and confocal microscopy,

Figure 4. Excitation (left) and emission (right)spectra of Cy2 (green), Cy3 (red), and Cy5 (purple).

Peak heights were normalized after the spectrawere obtained with an M-series spectrofluorometersystem from Photon Technology International, Inc.

Exci

tatio

n

Wavelength (nm)700650600550500450400300

0350

Fluo

resc

ence

Wavelength (nm)750700650600550500400

0450

Figure 4.




and therefore it should be used with mounting media containing an anti-fading agent suchas n-propyl gallate.

For flow cytometry, AMCA can be excited with a mercury lamp or with a water-cooled argonion laser which emits some lines in the UV. AMCA has been used mostly for multiple labelingsince there is minimal fluorescence overlap with green-fluorescing dyes and little or nooverlap with longer wavelength-emitting fluorophores. Applications for multiple labeling withthis probe include both immunofluorescence microscopy and flow cytometry. AMCA is notsuggested for single labeling in one-photon microscopy because of its relatively weak signaland rapid fading. However, AMCA has been found to be a bright and photostable dye for2-photon microscopy. In one-photon microscopy DyLight 405 is a better choice for multiplelabeling.

Cy2 conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peakaround 510 nm in the green region of the visible spectrum (Figure 2 and Table 1) like FITCconjugates (520 nm), but they are more photostable and less sensitive to pH changes thanFITC. However, for mounting in aqueous media we recommend Alexa Fluor® 488 as thepreferred green-fluorescing dye because it is brighter and more photostable than Cy2, FITC,and DyLight 488. A further disadvantage of using Cy2 with aqueous mounting media is itssensitivity to p-phenylenediamine, an anti-fading agent found in some commercial mountingmedia, which results in weak and diffused fluorescence after storage of stained slides. Themain advantage of Cy2 conjugates is increased fluorescence in plastic mounting media.Cy2 is available conjugated to a limited selection of antibodies (pages 68-70).

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluorescewith a peak around 519 nm (Figure 2 and Table 1). In aqueous mounting media they arebrighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommendedfor maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescingdye, except for protocols that include mounting in plastic mounting media.

FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all ofour antibodies and purified proteins, with the exception of streptavidin. Fluorescein

conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm (Figure 2and Table 1). Although less bright than other green-fluorescing dyes, FITC is still a widelyused fluorophore due to its long history. The major disadvantage of fluorescein is its rapidphotobleaching (fading), which can be mitigated by the use of an anti-fading agent in themounting medium. A better choice for many applications involving FITC is Alexa Fluor® 488because it is brighter and more photostable.

DTAF (Dichlorotriazinylamino fluorescein) is another form of fluorescein, with excitationand emission peaks identical to those of FITC. We use DTAF (instead of FITC) only forconjugation with streptavidin, since fluorescence from FITC is greatly quenched afterconjugation with streptavidin. This phenomenon is unique to streptavidin, and is notobserved with antibodies.

Cy3 is brighter, more photostable, and gives less background than other orange-redfluorescing dye conjugates. Cy3 conjugates can be excited maximally at 550 nm, withpeak emission at 570 nm (Figure 2 and Table 1). For fluorescence microscopy, Cy3 canbe visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitationand emission spectra are nearly identical to those of TRITC. We recommend Cy3 as abrighter alternative to TRITC. Cy3 can be excited to about 50% of maximum with an argonlaser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser(543 nm line) or mercury lamp (546 nm line). Cy3 has been used with fluorescein fordouble labeling; however, the use of a narrow band-pass emission filter for fluorescein isrecommended to minimize Cy3 fluorescence in the FITC filter set. Cy3 can also be pairedwith Alexa Fluor® 647 for multiple labeling when using a confocal microscope. However, abetter choice for multiple labeling is Rhodamine Red-X (Figure 2 and Table 1) because itsfluorescence is midway between a green fluorescing dye (like Alexa Fluor® 488) and a far-red-fluorescing dye like Alexa Fluor® 647 (Figure 5).

RRX (Rhodamine Red-X) conjugates have a peak of excitation at 570 nm and a peak ofemission at 590 nm (Figure 2 and Table 1). Although TRITC has been used traditionallywith FITC for double labeling, better color separation is achieved by using RRX or Alexa Fluor® 594. Rhodamine Red-X is particularly useful for 3- and 4-color labeling with

12


DyLight 405, Alexa Fluor® 488, and Alexa Fluor® 647 by using a confocal microscopeequipped with a 405 nm laser and a krypton/argon laser. Fluorescence from RRX lies aboutmidway between that of Alexa Fluor® 488 and Alexa Fluor® 647, and it shows little overlapwith either dye (Figure 5). The krypton-argon laser emits lines at 488 nm, 568 nm, and 647nm, which are optimal for exciting Alexa Fluor® 488, RRX, and Alexa Fluor® 647,respectively. By adding a 405 nm laser and a 420 nm emission filter, 4-color labeling ispossible using DyLight 405-conjugated secondary antibodies from JIR (Figure 5). Theseparation between all four dyes is perfect for 4-color labeling, and all four dyes are verybright.

Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm andfluoresce with a peak around 614 nm (Figure 2 and Table 1). They are brighter, morephotostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594 conjugatesare brighter than red-fluorescing conjugates, and they provide more color separation fromgreen-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the bestchoice for immunofluorescence detection in the deep-red region of the visible spectrum.

Alexa Fluor® 647-conjugated antibodies absorb light maximally around 651 nm andfluoresce maximally around 667 nm (Figures 2 and 3 and Table 1). They are brighter thanCy5 and DyLight 650 in aqueous mounting media. Alexa Fluor® 647- and APC-conjugatedsecondary antibodies are the best choice for flow cytometry when secondary antibodiesfluorescing at these wavelengths are desired. Alexa Fluor® 647 conjugates are the bestchoice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.

A significant advantage of using Alexa Fluor® 647 over lower wavelength-emitting dyesis the low autofluorescence of biological specimens in this region of the spectrum.However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore,Alexa Fluor® 647 is not recommended for use with conventional epifluorescentmicroscopes. It is most commonly visualized with a confocal microscope equipped withan appropriate laser for excitation and a far-red detector. Alexa Fluor® 647 conjugatesare less expensive alternatives to allophycocyanin conjugates for flow cytometry.

Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm (Figure 2and Table 1). They can be excited to about 98% of maximum with a krypton/argon laser(647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 canbe used with a variety of other fluorophores for multiple labeling due to a wide separation ofits emission from that of shorter wavelength-emitting fluorophores. However, we recommendAlexa Fluor® 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 inaqueous mounting media. The reverse is true when mounting in non-polar, plastic mountingmedia, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor® 647and DyLight 650. Cy5 is available conjugated to a limited selection of antibodies, see pages68-70.

Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Westernblots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with apeak around 684 nm and fluoresce with a peak around 702 nm (Figures 2 and 3 and Table1). Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce

Fluo

resc

ence

Wavelength (nm)700 750650600550500450350

0400

Figure 5.

Figure 5. Emission spectra foroptimal 4-color imaging usingDyLight 405 (blue), Alexa Fluor®488(green), Rhodamine Red-X (red),and Alexa Fluor® 647 (purple).




at a peak around 803 nm. They are the best choice for highly sensitive single or doublelabeling with fluorescence imaged in a LI-COR Odyssey® imager (see page 64 for moreinformation).

Biotin-SP (long spacer)Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin andthe protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used inenzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugatedantibodies without the spacer. This is especially notable when Biotin-SP conjugatedantibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, thelong spacer extends the biotin moiety away from the antibody surface, making it moreaccessible to binding sites on streptavidin. Biotinylated antibodies require an additionalreagent for visualization. We offer streptavidin (page 71) and Mouse Anti-Biotin conjugatedto fluorophores and enzymes (page 76-77).

EnzymesHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane andKawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates arecommonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purifiedanti-horseradish peroxidase and conjugates are available for detection of horseradishperoxidase antigen or for signal amplification of HRP-containing reagents. Forimmunostaining of mammalian cells, an advantage of using anti-horseradish peroxidaseis reduced background, since the antibody does not recognize the endogenousperoxidase-like enzymes found in those cells.

Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified methodof Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugatescontain heterogeneous, high molecular weight complexes. They are sensitive reagents forsolid-phase immunoassays such as ELISA and Western blotting. Although alkalinephosphatase conjugates are sometimes used for immunohistochemistry, penetration intowhole mount tissues may be limited by their large sizes.

14 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Whole IgG Affinity-Purified Antibodies

Antibody Description

Goat Anti-Bovine IgG (H+L)***

Goat Anti-Bovine IgG (H+L)***(min X Ar Hms, Hu, Ms, Rat Sr Prot)

Rabbit Anti-Bovine IgG (H+L)***

Goat Anti-Cat IgG (H+L)

Goat Anti-Cat IgG, Fc fragment specific

Goat Anti-Cat IgG, F(ab')2 fragment specific

Donkey Anti-Chicken IgY† (IgG)(H+L) (min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Goat Anti-Chicken IgY† (IgG)(H+L) (min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Rabbit Anti-Chicken IgY† (IgG) (H+L)

Rabbit Anti-Chicken IgY† (IgG), Fc fragment specific

Rabbit Anti-Chicken IgY† (IgG), F(ab')2 fragment specific

Rabbit Anti-Dog IgG (H+L)

Unconjugated DyLight 405 A=400, E=421

Coumarin AMCAA=350, E=450

Alexa Fluor® 488 A=493, E=519

2.0 mg101-005-003

1.5 mg101-005-165

2.0 mg301-005-003

2.0 mg102-005-003

2.0 mg102-005-008

2.0 mg102-005-006

1.0 mg703-005-155

1.0 mg103-005-155

2.0 mg303-005-003

2.0 mg303-005-008

2.0 mg303-005-006

2.0 mg304-005-003

1.5 mg101-475-003

1.0 mg101-475-165

1.0 mg301-475-003

1.5 mg102-475-003

1.5 mg 102-475-008

1.5 mg 102-475-006

0.5 mg703-475-155

0.5 mg103-475-155

1.0 mg303-475-003

1.0 mg303-475-008

1.0 mg303-475-006

1.0 mg304-475-003

2.0 mg101-155-003

1.5 mg101-155-165

1.5 mg301-155-003

2.0 mg102-155-003

2.0 mg102-155-008

2.0 mg102-155-006

0.5 mg703-155-155

0.5 mg103-155-155

1.5 mg303-155-003

1.5 mg303-155-008

1.5 mg303-155-006

1.5 mg304-155-003

1.5 mg101-545-003

1.0 mg101-545-165

1.0 mg301-545-003

1.5 mg102-545-003

1.5 mg102-545-008

1.5 mg 102-545-006

0.5 mg703-545-155

0.5 mg103-545-155

1.0 mg303-545-003

1.0 mg303-545-008

1.0 mg303-545-006

1.0 mg304-545-003

Whole IgG ANTI-BOVINE

Whole IgG ANTI-CAT

Whole IgG ANTI-CHICKEN

Whole IgG ANTI-DOG

ML*

ML*

ML*

15



AlkalinePhosphatase

Biotin-SP(long spacer)

HorseradishPeroxidase

RhodamineRed-X RRXA=570, E=590

Cyanine Cy3A=550, E=570

Fluorescein FITCA=492, E=520



1.0 ml101-055-003

1.0 ml101-055-165

1.0 ml301-055-003

1.0 ml102-055-003

1.0 ml102-055-008

1.0 ml102-055-006

0.5 ml703-055-155

0.5 ml103-055-155

1.0 ml303-055-003

1.0 ml303-055-008

1.0 ml303-055-006

1.0 ml304-055-003

2.0 ml101-035-003

1.5 ml101-035-165

1.5 ml301-035-003

2.0 ml102-035-003

2.0 ml102-035-008

2.0 ml102-035-006

0.5 ml703-035-155

0.5 ml103-035-155

1.5 ml303-035-003

1.5 ml303-035-008

1.5 ml303-035-006

1.5 ml304-035-003

2.0 ml101-065-003

1.5 ml101-065-165

1.5 ml301-065-003

2.0 ml102-065-003

2.0 ml102-065-008

2.0 ml102-065-006

0.5 ml703-065-155

0.5 ml103-065-155

1.5 ml303-065-003

1.5 ml303-065-008

1.5 ml303-065-006

1.5 ml304-065-003

1.5 mg101-605-003

1.0 mg101-605-165

1.0 mg301-605-003

1.5 mg102-605-003

1.5 mg102-605-008

1.5 mg 102-605-006

0.5 mg703-605-155

0.5 mg103-605-155

1.0 mg303-605-003

1.0 mg303-605-008

1.0 mg303-605-006

1.0 mg304-605-003

1.5 mg101-585-003

1.0 mg101-585-165

1.0 mg301-585-003

1.5 mg102-585-003

1.5 mg 102-585-008

1.5 mg 102-585-006

0.5 mg703-585-155

0.5 mg103-585-155

1.0 mg303-585-003

1.0 mg303-585-008

1.0 mg303-585-006

1.0 mg304-585-003

2.0 mg101-295-003

1.5 mg101-295-165

1.5 mg301-295-003

2.0 mg102-295-003

2.0 mg102-295-008

2.0 mg102-295-006

0.5 mg703-295-155

0.5 mg103-295-155

1.5 mg303-295-003

1.5 mg303-295-008

1.5 mg303-295-006

1.5 mg304-295-003

2.0 mg101-165-003

1.5 mg 101-165-165

1.5 mg301-165-003

2.0 mg102-165-003

2.0 mg102-165-008

2.0 mg102-165-006

0.5 mg703-165-155

0.5 mg103-165-155

1.5 mg303-165-003

1.5 mg303-165-008

1.5 mg303-165-006

1.5 mg304-165-003

2.0 mg101-095-003

1.5 mg101-095-165

1.5 mg301-095-003

2.0 mg102-095-003

2.0 mg102-095-008

2.0 mg102-095-006

0.5 mg703-095-155

0.5 mg103-095-155

1.5 mg303-095-003

1.5 mg303-095-008

1.5 mg303-095-006

1.5 mg304-095-003

www.jacksonimmuno.com Telephone: 800-367-5296 Fax: 610-869-0171

16 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).



Rabbit Anti-Dog IgG, Fc fragment specific

Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Donkey Anti-Goat IgG (H+L)***

Donkey Anti-Goat IgG (H+L)*** (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Mouse Anti-Goat IgG (H+L)*** (min X Hu, Ms, Rb Sr Prot)

IgG Fraction Monoclonal Mouse Anti-Goat IgG, Light Chain Specific***(min X Hrs, Hu, Ms, Rb, Rat Ig)

Rabbit Anti-Goat IgG (H+L)***

Rabbit Anti-Goat IgG (H+L)***(min X Hu Sr Prot)

Rabbit Anti-Goat IgG, Fc fragment specific***

Rabbit Anti-Goat IgG, Fc fragment specific***(min X Hu Sr Prot)

Rabbit Anti-Goat IgG, F(ab')2 fragment specific***

Rabbit Anti-Goat IgG, F(ab')2 fragment specific***(min X Hu Sr Prot)

Donkey Anti-Guinea Pig IgG (H+L) (min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Whole IgG ANTI-DOG




2.0 mg304-005-008

1.0 mg805-005-180

1.0 mg 705-005-003

1.0 mg705-005-147

1.5 mg205-005-108

1.0 mg 205-002-176

2.0 mg305-005-003

1.5 mg305-005-045

2.0 mg305-005-008

1.5 mg305-005-046

2.0 mg305-005-006

1.5 mg305-005-047

1.0 mg706-005-148

1.0 mg304-475-008

0.5 mg805-475-180

1.0 mg 705-475-003

0.5 mg705-475-147

1.0 mg205-475-108

1.0 mg305-475-003

1.0 mg305-475-045

1.0 mg305-475-008

1.0 mg305-475-046

1.0 mg305-475-006

1.0 mg305-475-047

0.5 mg706-475-148

1.5 mg304-155-008

0.5 mg805-155-180

1.0 mg 705-155-003

0.5 mg705-155-147

1.0 mg205-155-108

1.5 mg305-155-003

1.0 mg305-155-045

1.5 mg305-155-008

1.0 mg305-155-046

1.5 mg305-155-006

1.0 mg305-155-047

0.5 mg706-155-148

1.0 mg304-545-008

0.5 mg805-545-180

1.0 mg 705-545-003

0.5 mg705-545-147

1.0 mg205-545-108

0.5 mg 205-542-176

1.0 mg305-545-003

1.0 mg305-545-045

1.0 mg305-545-008

1.0 mg305-545-046

1.0 mg305-545-006

1.0 mg305-545-047

0.5 mg706-545-148

Whole IgG ANTI-GOAT

Whole IgG ANTI-GUINEA PIG

ML*

ML*

ML*

ML*

17



AlkalinePhosphatase








1.0 ml304-055-008

0.5 ml805-055-180

1.0 ml 705-055-003

0.5 ml705-055-147

0.5 ml205-055-108

0.5 ml 205-055-176

1.0 ml305-055-003

0.5 ml305-055-045

1.0 ml305-055-008

0.5 ml305-055-046

1.0 ml305-055-006

0.5 ml305-055-047

0.5 ml706-055-148

1.5 ml304-065-008

0.5 ml805-065-180

1.0 ml 705-065-003

0.5 ml705-065-147

1.0 ml205-065-108

0.5 ml 205-062-176

1.5 ml305-065-003

1.0 ml305-065-045

1.5 ml305-065-008

1.0 ml305-065-046

1.5 ml305-065-006

1.0 ml305-065-047

0.5 ml706-065-148

1.5 ml304-035-008

0.5 ml805-035-180

1.0 ml 705-035-003

0.5 ml705-035-147

1.0 ml205-035-108

0.5 ml 205-032-176

1.5 ml305-035-003

1.0 ml305-035-045

1.5 ml305-035-008

1.0 ml305-035-046

1.5 ml305-035-006

1.0 ml305-035-047

0.5 ml706-035-148

1.0 mg304-605-008

0.5 mg805-605-180

1.0 mg 705-605-003

0.5 mg705-605-147

1.0 mg205-605-108

0.5 mg 205-602-176

1.0 mg305-605-003

1.0 mg305-605-045

1.0 mg305-605-008

1.0 mg305-605-046

1.0 mg305-605-006

1.0 mg305-605-047

0.5 mg706-605-148

1.0 mg304-585-008

0.5 mg805-585-180

1.0 mg 705-585-003

0.5 mg705-585-147

1.0 mg205-585-108

0.5 mg 205-582-176

1.0 mg305-585-003

1.0 mg305-585-045

1.0 mg305-585-008

1.0 mg305-585-046

1.0 mg305-585-006

1.0 mg305-585-047

0.5 mg706-585-148

1.5 mg304-295-008

0.5 mg805-295-180

1.0 mg 705-295-003

0.5 mg705-295-147

1.0 mg205-295-108

1.5 mg305-295-003

1.0 mg305-295-045

1.5 mg305-295-008

1.0 mg305-295-046

1.5 mg305-295-006

1.0 mg305-295-047

0.5 mg706-295-148

1.5 mg304-165-008

0.5 mg805-165-180

1.0 mg 705-165-003

0.5 mg705-165-147

1.0 mg205-165-108

0.5 mg 205-162-176

1.5 mg305-165-003

1.0 mg305-165-045

1.5 mg305-165-008

1.0 mg305-165-046

1.5 mg305-165-006

1.0 mg305-165-047

0.5 mg706-165-148

1.5 mg304-095-008

0.5 mg805-095-180

1.0 mg705-095-003

0.5 mg705-095-147

1.0 mg205-095-108

1.5 mg305-095-003

1.0 mg305-095-045

1.5 mg305-095-008

1.0 mg305-095-046

1.5 mg305-095-006

1.0 mg305-095-047

0.5 mg706-095-148


18 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).



Goat Anti-Guinea Pig IgG (H+L)

Goat Anti-Guinea Pig IgG, Fc fragment specific

Goat Anti-Guinea Pig IgG, F(ab')2 fragment specific

Goat Anti-Armenian Hamster IgG (H+L)(min X Bov Sr Prot)

Goat Anti-Armenian Hamster IgG (H+L)(min X Bov, Hu, Ms, Rb, Rat Sr Prot)**

Goat Anti-Syrian Hamster IgG (H+L)(min X Bov, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Rabbit Anti-Syrian Hamster IgG (H+L)

Goat Anti-Horse IgG (H+L)

Goat Anti-Horse IgG, Fc fragment specific

Rabbit Anti-Horse IgG (H+L)***

Donkey Anti-Human IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)

Whole IgG ANTI-GUINEA PIG




2.0 mg106-005-003

2.0 mg106-005-008

2.0 mg106-005-006

1.5 mg127-005-099

1.0 mg127-005-160

1.0 mg107-005-142

2.0 mg307-005-003

2.0 mg108-005-003

2.0 mg108-005-008

2.0 mg308-005-003

1.0 mg709-005-149

1.5 mg106-475-003

1.5 mg 106-475-008

1.5 mg 106-475-006

1.0 mg127-475-099

0.5 mg127-475-160

1.0 mg107-475-142

1.0 mg307-475-003

1.5 mg 108-475-003

1.5 mg108-475-008

1.0 mg308-475-003

0.5 mg709-475-149

2.0 mg106-155-003

2.0 mg106-155-008

2.0 mg106-155-006

1.5 mg127-155-099

0.5 mg127-155-160

1.0 mg107-155-142

1.5 mg307-155-003

2.0 mg108-155-003

2.0 mg108-155-008

1.5 mg308-155-003

0.5 mg709-155-149

1.5 mg106-545-003

1.5 mg 106-545-008

1.5 mg 106-545-006

1.0 mg127-545-099

0.5 mg127-545-160

1.0 mg107-545-142

1.0 mg307-545-003

1.5 mg 108-545-003

1.5 mg108-545-008

1.0 mg308-545-003

0.5 mg709-545-149

Whole IgG ANTI-HAMSTER (Armenian Hamster)

Whole IgG ANTI-HAMSTER (Syrian Hamster)

Whole IgG ANTI-HORSE

Whole IgG ANTI-HUMANML*

19



AlkalinePhosphatase








1.0 ml106-055-003

1.0 ml106-055-008

1.0 ml106-055-006

1.0 ml127-055-099

0.5 ml127-055-160

0.5 ml107-055-142

1.0 ml307-055-003

1.0 ml108-055-003

1.0 ml108-055-008

1.0 ml308-055-003

0.5 ml709-055-149

2.0 ml106-065-003

2.0 ml106-065-008

2.0 ml106-065-006

1.5 ml127-065-099

0.5 ml127-065-160

1.0 ml107-065-142

1.5 ml307-065-003

2.0 ml108-065-003

2.0 ml108-065-008

1.5 ml308-065-003

0.5 ml709-065-149

2.0 ml106-035-003

2.0 ml106-035-008

2.0 ml106-035-006

1.5 ml127-035-099

0.5 ml127-035-160

1.0 ml107-035-142

1.5 ml307-035-003

2.0 ml108-035-003

2.0 ml108-035-008

1.5 ml308-035-003

0.5 ml709-035-149

1.5 mg106-605-003

1.5 mg 106-605-008

1.5 mg106-605-006

1.0 mg127-605-099

0.5 mg127-605-160

1.0 mg107-605-142

1.0 mg307-605-003

1.5 mg 108-605-003

1.5 mg108-605-008

1.0 mg308-605-003

0.5 mg709-605-149

1.5 mg106-585-003

1.5 mg 106-585-008

1.5 mg 106-585-006

1.0 mg127-585-099

0.5 mg127-585-160

1.0 mg107-585-142

1.0 mg307-585-003

1.5 mg 108-585-003

1.5 mg108-585-008

1.0 mg308-585-003

0.5 mg709-585-149

2.0 mg106-295-003

2.0 mg106-295-008

2.0 mg106-295-006

1.5 mg127-295-099

0.5 mg127-295-160

1.0 mg107-295-142

1.5 mg307-295-003

2.0 mg108-295-003

2.0 mg108-295-008

1.5 mg308-295-003

0.5 mg709-295-149

2.0 mg106-165-003

2.0 mg106-165-008

2.0 mg106-165-006

1.5 mg127-165-099

0.5 mg127-165-160

1.0 mg107-165-142

1.5 mg307-165-003

2.0 mg108-165-003

2.0 mg108-165-008

1.5 mg308-165-003

0.5 mg709-165-149

2.0 mg106-095-003

2.0 mg106-095-008

2.0 mg106-095-006

1.5 mg127-095-099

0.5 mg127-095-160

1.0 mg107-095-142

1.5 mg307-095-003

2.0 mg108-095-003

2.0 mg108-095-008

1.5 mg308-095-003

0.5 mg709-095-149

Whole IgG ANTI-HAMSTER (Armenian Hamster)

Whole IgG ANTI-HAMSTER (Syrian Hamster)

Whole IgG ANTI-HORSE

Whole IgG ANTI-HUMAN


20



Donkey Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot)

Donkey Anti-Human IgM, Fc5µ fragment specific(min X Bov, Hrs Sr Prot)

Goat Anti-Human IgG (H+L)

Goat Anti-Human IgG (H+L)(min X Bov, Hrs, Ms Sr Prot)

Goat Anti-Human IgG, Fcγ fragment specific

Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot)

Goat Anti-Human IgG, F(ab')2 fragment specific

Goat Anti-Human IgG, F(ab')2 fragment specific(min X Bov, Hrs, Ms Sr Prot)

Goat Anti-Human IgG + IgM (H+L)

Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot)

Goat Anti-Human IgA + IgG + IgM (H+L)

Goat Anti-Human IgM, Fc5µ fragment specific

Goat Anti-Human IgM, Fc5µ fragment specific(min X Bov Sr Prot)

Goat Anti-Human Serum IgA, α chain specific

Mouse Anti-Human IgG (H+L) (min X Ms Sr Prot)

1.0 mg 709-005-098

1.5 mg709-005-073

2.0 mg109-005-003

1.5 mg109-005-088

2.0 mg109-005-008

1.0 mg109-005-098

2.0 mg109-005-006

1.5 mg109-005-097

2.0 mg109-005-044

1.5 mg109-005-127

2.0 mg109-005-064

2.0 mg109-005-043

1.5 mg109-005-129

2.0 mg109-005-011

2.0 mg209-005-082

Whole IgG ANTI-HUMAN1.0 mg

709-475-098

1.0 mg709-475-073

1.5 mg109-475-003

1.0 mg109-475-088

1.5 mg109-475-008

1.0 mg109-475-098

1.5 mg109-475-006

1.0 mg109-475-097

1.5 mg109-475-044

1.0 mg109-475-127

1.5 mg109-475-064

1.5 mg109-475-043

1.0 mg109-475-129

1.5 mg109-475-011

1.0 mg209-475-082

1.0 mg 709-155-098

1.5 mg709-155-073

2.0 mg109-155-003

1.5 mg109-155-088

2.0 mg109-155-008

1.0 mg109-155-098

2.0 mg109-155-006

1.5 mg109-155-097

2.0 mg109-155-044

1.5 mg109-155-127

2.0 mg109-155-064

2.0 mg109-155-043

1.5 mg109-155-129

2.0 mg109-155-011

1.5 mg209-155-082

1.0 mg 709-545-098

1.0 mg709-545-073

1.5 mg109-545-003

1.0 mg109-545-088

1.5 mg109-545-008

1.0 mg109-545-098

1.5 mg109-545-006

1.0 mg109-545-097

1.5 mg109-545-044

1.0 mg109-545-127

1.5 mg109-545-064

1.5 mg109-545-043

1.0 mg109-545-129

1.5 mg109-545-011

1.0 mg209-545-082




21



1.0 ml 709-055-098

1.0 ml709-055-073

1.0 ml109-055-003

1.0 ml109-055-088

1.0 ml109-055-008

1.0 ml109-055-098

1.0 ml109-055-006

1.0 ml109-055-097

1.0 ml109-055-044

1.0 ml109-055-127

1.0 ml109-055-064

1.0 ml109-055-043

1.0 ml109-055-129

1.0 ml109-055-011

1.0 ml209-055-082

1.0 ml709-065-098

1.5 ml709-065-073

2.0 ml109-065-003

1.5 ml109-065-088

2.0 ml109-065-008

1.0 ml109-065-098

2.0 ml109-065-006

1.5 ml109-065-097

2.0 ml109-065-044

1.5 ml109-065-127

2.0 ml109-065-064

2.0 ml109-065-043

1.5 ml109-065-129

2.0 ml109-065-011

1.5 ml209-065-082

1.0 ml709-035-098

1.5 ml709-035-073

2.0 ml109-035-003

1.5 ml109-035-088

2.0 ml109-035-008

1.0 ml109-035-098

2.0 ml109-035-006

1.5 ml109-035-097

2.0 ml109-035-044

1.5 ml109-035-127

2.0 ml109-035-064

2.0 ml109-035-043

1.5 ml109-035-129

2.0 ml109-035-011

1.5 ml209-035-082

1.0 mg 709-605-098

1.0 mg709-605-073

1.5 mg109-605-003

1.0 mg109-605-088

1.5 mg109-605-008

1.0 mg109-605-098

1.5 mg109-605-006

1.0 mg109-605-097

1.5 mg109-605-044

1.0 mg109-605-127

1.5 mg109-605-064

1.5 mg109-605-043

1.0 mg109-605-129

1.5 mg109-605-011

1.0 mg209-605-082

1.0 mg 709-585-098

1.0 mg709-585-073

1.5 mg109-585-003

1.0 mg109-585-088

1.5 mg109-585-008

1.0 mg109-585-098

1.5 mg109-585-006

1.0 mg109-585-097

1.5 mg109-585-044

1.0 mg109-585-127

1.5 mg109-585-064

1.5 mg109-585-043

1.0 mg109-585-129

1.5 mg109-585-011

1.0 mg209-585-082

1.0 mg 709-295-098

1.5 mg709-295-073

2.0 mg109-295-003

1.5 mg109-295-088

2.0 mg 109-295-008

1.0 mg109-295-098

2.0 mg109-295-006

1.5 mg109-295-097

2.0 mg109-295-044

1.5 mg109-295-127

2.0 mg109-295-064

2.0 mg109-295-043

1.5 mg109-295-129

2.0 mg109-295-011

1.5 mg209-295-082

1.0 mg709-165-098

1.5 mg709-165-073

2.0 mg109-165-003

1.5 mg109-165-088

2.0 mg109-165-008

1.0 mg109-165-098

2.0 mg109-165-006

1.5 mg109-165-097

2.0 mg109-165-044

1.5 mg109-165-127

2.0 mg109-165-064

2.0 mg109-165-043

1.5 mg109-165-129

2.0 mg109-165-011

1.5 mg209-165-082

1.0 mg 709-095-098

1.5 mg709-095-073

2.0 mg109-095-003

1.5 mg109-095-088

2.0 mg109-095-008

1.0 mg109-095-098

2.0 mg109-095-006

1.5 mg109-095-097

2.0 mg109-095-044

1.5 mg109-095-127

2.0 mg109-095-064

2.0 mg109-095-043

1.5 mg109-095-129

2.0 mg109-095-011

1.5 mg209-095-082

AlkalinePhosphatase









22 ** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).



Mouse Anti-Human IgG (H+L)(min X Bov, Hrs, Ms Sr Prot)

Mouse Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot)

Mouse Anti-Human IgG, F(ab')2 fragment specific(min X Bov, Hrs, Ms Sr Prot)

Rabbit Anti-Human IgG (H+L)

Rabbit Anti-Human IgG (H+L)(min X Ms Sr Prot)

Rabbit Anti-Human IgG, Fcγ fragment specific

Rabbit Anti-Human IgG, F(ab')2 fragment specific

Rabbit Anti-Human IgG + IgM (H+L) (min X Ms Sr Prot)

Rabbit Anti-Human IgA + IgG + IgM (H+L)

Rabbit Anti-Human IgM, Fc5µ fragment specific(min X Ms Sr Prot)

Rabbit Anti-Human Serum IgA, α chain specific

Rabbit Anti-Human Lactoferrin

Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)**

Whole IgG ANTI-HUMAN




Whole IgG ANTI-MOUSE

1.5 mg209-005-088

1.5 mg209-005-098

1.5 mg209-005-097

2.0 mg309-005-003

1.5 mg309-005-082

2.0 mg309-005-008

2.0 mg309-005-006

1.5 mg309-005-107

2.0 mg309-005-064

1.5 mg309-005-095

2.0 mg309-005-011

2.0 mg309-005-015

1.0 mg715-005-150

1.0 mg715-005-151

1.0 mg209-475-088

1.0 mg209-475-098

1.0 mg209-475-097

1.0 mg309-475-003

1.0 mg309-475-082

1.0 mg309-475-008

1.0 mg309-475-006

1.0 mg309-475-107

1.0 mg309-475-064

1.0 mg309-475-095

1.0 mg309-475-011

1.0 mg309-475-015

0.5 mg715-475-150

0.5 mg 715-475-151

1.0 mg209-155-088

1.0 mg209-155-098

1.0 mg209-155-097

1.5 mg309-155-003

1.0 mg309-155-082

1.5 mg309-155-008

1.5 mg309-155-006

1.0 mg309-155-107

1.5 mg309-155-064

1.0 mg309-155-095

1.5 mg309-155-011

1.5 mg309-155-015

0.5 mg715-155-150

0.5 mg715-155-151

1.0 mg209-545-088

1.0 mg209-545-098

1.0 mg209-545-097

1.0 mg309-545-003

1.0 mg309-545-082

1.0 mg309-545-008

1.0 mg309-545-006

1.0 mg309-545-107

1.0 mg309-545-064

1.0 mg309-545-095

1.0 mg309-545-011

1.0 mg309-545-015

0.5 mg715-545-150

0.5 mg 715-545-151

ML*

ML*

23



AlkalinePhosphatase









0.5 ml209-055-088

0.5 ml209-055-098

0.5 ml209-055-097

1.0 ml309-055-003

0.5 ml309-055-082

1.0 ml309-055-008

1.0 ml309-055-006

0.5 ml309-055-107

1.0 ml309-055-064

0.5 ml309-055-095

1.0 ml309-055-011

1.0 ml309-055-015

0.5 ml715-055-150

0.5 ml715-055-151

1.0 ml209-065-088

1.0 ml209-065-098

1.0 ml209-065-097

1.5 ml309-065-003

1.0 ml309-065-082

1.5 ml309-065-008

1.5 ml309-065-006

1.0 ml309-065-107

1.5 ml309-065-064

1.0 ml309-065-095

1.5 ml309-065-011

1.5 ml309-065-015

0.5 ml715-065-150

0.5 ml715-065-151

1.0 ml209-035-088

1.0 ml209-035-098

1.0 ml209-035-097

1.5 ml309-035-003

1.0 ml309-035-082

1.5 ml309-035-008

1.5 ml309-035-006

1.0 ml309-035-107

1.5 ml309-035-064

1.0 ml309-035-095

1.5 ml309-035-011

1.5 ml309-035-015

0.5 ml715-035-150

0.5 ml715-035-151

1.0 mg209-605-088

1.0 mg209-605-098

1.0 mg209-605-097

1.0 mg309-605-003

1.0 mg309-605-082

1.0 mg309-605-008

1.0 mg309-605-006

1.0 mg309-605-107

1.0 mg309-605-064

1.0 mg309-605-095

1.0 mg309-605-011

1.0 mg309-605-015

0.5 mg715-605-150

0.5 mg 715-605-151

1.0 mg209-585-088

1.0 mg209-585-098

1.0 mg209-585-097

1.0 mg309-585-003

1.0 mg309-585-082

1.0 mg309-585-008

1.0 mg309-585-006

1.0 mg309-585-107

1.0 mg309-585-064

1.0 mg309-585-095

1.0 mg309-585-011

1.0 mg309-585-015

0.5 mg715-585-150

0.5 mg 715-585-151

1.0 mg209-295-088

1.0 mg209-295-098

1.0 mg209-295-097

1.5 mg309-295-003

1.0 mg309-295-082

1.5 mg309-295-008

1.5 mg309-295-006

1.0 mg309-295-107

1.5 mg309-295-064

1.0 mg309-295-095

1.5 mg309-295-011

1.5 mg309-295-015

0.5 mg715-295-150

0.5 mg715-295-151

1.0 mg209-165-088

1.0 mg209-165-098

1.0 mg209-165-097

1.5 mg309-165-003

1.0 mg309-165-082

1.5 mg309-165-008

1.5 mg309-165-006

1.0 mg309-165-107

1.5 mg309-165-064

1.0 mg309-165-095

1.5 mg309-165-011

1.5 mg309-165-015

0.5 mg715-165-150

0.5 mg715-165-151

1.0 mg209-095-088

1.0 mg209-095-098

1.0 mg209-095-097

1.5 mg309-095-003

1.0 mg309-095-082

1.5 mg309-095-008

1.5 mg309-095-006

1.0 mg309-095-107

1.5 mg309-095-064

1.0 mg309-095-095

1.5 mg309-095-011

1.5 mg309-095-015

0.5 mg715-095-150

0.5 mg715-095-151


24 ** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).‡ This antibody reacts primarily with kappa light chains. It is not suitable for detection of primary antibodies with lambda light chains.



Donkey Anti-Mouse IgM, µ chain specific

Donkey Anti-Mouse IgM, µ chain specific(min X Hu, Bov, Hrs, Rat Sr Prot)**

Goat Anti-Mouse IgG (H+L)

Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Sw Sr Prot)

Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Rat Sr Prot)**

Goat Anti-Mouse IgG, Light Chain Specific‡

(min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig)

Goat Anti-Mouse IgG, Fcγ fragment specific

Goat Anti-Mouse IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG, Fcγ Subclass 1 specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ Subclass 2a specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ Subclass 2b specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ Subclass 2c specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ Subclass 3 specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific(min X Hu, Bov, Rb Sr Prot)

2.0 mg715-005-020

1.0 mg715-005-140

2.0 mg115-005-003

1.5 mg115-005-062

1.5 mg115-005-146

1.0 mg115-005-166

1.0 mg115-005-174

2.0 mg115-005-008

1.5 mg115-005-071

1.0 mg 115-005-205

1.0 mg115-005-206

1.0 mg115-005-207

1.0 mg115-005-208

1.0 mg115-005-209

1.0 mg115-005-164

Whole IgG ANTI-MOUSE1.5 mg

715-475-020

0.5 mg715-475-140

1.5 mg 115-475-003

1.0 mg115-475-062

1.0 mg115-475-146

0.5 mg115-475-166

1.5 mg115-475-008

1.0 mg115-475-071

0.5 mg 115-475-205

0.5 mg115-475-206

0.5 mg115-475-207

0.5 mg115-475-208

0.5 mg 115-475-209

1.0 mg115-475-164

2.0 mg715-155-020

0.5 mg715-155-140

2.0 mg115-155-003

1.5 mg115-155-062

1.5 mg115-155-146

0.5 mg115-155-166

2.0 mg115-155-008

1.5 mg115-155-071

0.5 mg115-155-205

0.5 mg115-155-206

0.5 mg115-155-207

0.5 mg115-155-208

0.5 mg115-155-209

1.0 mg115-155-164

1.5 mg715-545-020

0.5 mg715-545-140

1.5 mg115-545-003

1.0 mg115-545-062

1.0 mg115-545-146

0.5 mg115-545-166

0.5 mg115-545-174

1.5 mg115-545-008

1.0 mg115-545-071

0.5 mg 115-545-205

0.5 mg115-545-206

0.5 mg115-545-207

0.5 mg115-545-208

0.5 mg 115-545-209

1.0 mg115-545-164




ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

25



1.0 ml715-055-020

0.5 ml715-055-140

1.0 ml115-055-003

1.0 ml115-055-062

1.0 ml115-055-146

0.5 ml115-055-166

0.5 ml115-055-174

1.0 ml115-055-008

1.0 ml115-055-071

0.5 ml 115-055-205

0.5 ml115-055-206

0.5 ml115-055-207

0.5 ml115-055-208

0.5 ml 115-055-209

0.5 ml115-055-164

2.0 ml715-065-020

0.5 ml715-065-140

2.0 ml115-065-003

1.5 ml115-065-062

1.5 ml115-065-146

0.5 ml115-065-166

0.5 ml 115-065-174

2.0 ml115-065-008

1.5 ml115-065-071

0.5 ml115-065-205

0.5 ml115-065-206

0.5 ml115-065-207

0.5 ml115-065-208

0.5 ml115-065-209

1.0 ml115-065-164

2.0 ml715-035-020

0.5 ml715-035-140

2.0 ml115-035-003

1.5 ml115-035-062

1.5 ml115-035-146

0.5 ml115-035-166

0.5 ml 115-035-174

2.0 ml115-035-008

1.5 ml115-035-071

0.5 ml 115-035-205

0.5 ml115-035-206

0.5 ml115-035-207

0.5 ml115-035-208

0.5 ml 115-035-209

1.0 ml115-035-164

1.5 mg715-605-020

0.5 mg715-605-140

1.5 mg115-605-003

1.0 mg115-605-062

1.0 mg115-605-146

0.5 mg115-605-166

0.5 mg115-605-174

1.5 mg115-605-008

1.0 mg115-605-071

0.5 mg115-605-205

0.5 mg115-605-206

0.5 mg115-605-207

0.5 mg115-605-208

0.5 mg 115-605-209

1.0 mg115-605-164

1.5 mg715-585-020

0.5 mg715-585-140

1.5 mg115-585-003

1.0 mg115-585-062

1.0 mg115-585-146

0.5 mg115-585-166

0.5 mg115-585-174

1.5 mg115-585-008

1.0 mg115-585-071

0.5 mg 115-585-205

0.5 mg115-585-206

0.5 mg115-585-207

0.5 mg115-585-208

0.5 mg 115-585-209

1.0 mg115-585-164

2.0 mg715-295-020

0.5 mg715-295-140

2.0 mg115-295-003

1.5 mg115-295-062

1.5 mg115-295-146

0.5 mg115-295-166

2.0 mg115-295-008

1.5 mg115-295-071

0.5 mg 115-295-205

0.5 mg115-295-206

0.5 mg115-295-207

0.5 mg115-295-208

0.5 mg 115-295-209

1.0 mg115-295-164

2.0 mg715-165-020

0.5 mg715-165-140

2.0 mg115-165-003

1.5 mg115-165-062

1.5 mg115-165-146

0.5 mg115-165-166

0.5 mg115-165-174

2.0 mg115-165-008

1.5 mg115-165-071

0.5 mg115-165-205

0.5 mg115-165-206

0.5 mg115-165-207

0.5 mg115-165-208

0.5 mg 115-165-209

1.0 mg115-165-164

2.0 mg715-095-020

0.5 mg715-095-140

2.0 mg115-095-003

1.5 mg115-095-062

1.5 mg115-095-146

0.5 mg115-095-166

2.0 mg115-095-008

1.5 mg115-095-071

0.5 mg115-095-205

0.5 mg115-095-206

0.5 mg115-095-207

0.5 mg115-095-208

0.5 mg 115-095-209

1.0 mg115-095-164

AlkalinePhosphatase









26 * ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).



Goat Anti-Mouse IgG, F(ab')2 fragment specific

Goat Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG + IgM (H+L)

Goat Anti-Mouse IgG + IgM (H+L) (min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgM, µ chain specific

Goat Anti-Mouse IgM, µ chain specific(min X Hu, Bov, Hrs Sr Prot)

Rabbit Anti-Mouse IgG (H+L)

Rabbit Anti-Mouse IgG (H+L)(min X Hu Sr Prot)

Rabbit Anti-Mouse IgG, Fcγ fragment specific

Rabbit Anti-Mouse IgG, Fcγ fragment specific(min X Hu Sr Prot)

Rabbit Anti-Mouse IgG, F(ab')2 fragment specific

Rabbit Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu Sr Prot)

Rabbit Anti-Mouse IgG + IgM (H+L)

Rabbit Anti-Mouse IgG + IgM (H+L) (min X Hu Sr Prot)

Rabbit Anti-Mouse IgM, µ chain specific

2.0 mg115-005-006

1.5 mg115-005-072

2.0 mg115-005-044

1.5 mg 115-005-068

2.0 mg115-005-020

1.0 mg 115-005-075

2.0 mg315-005-003

1.5 mg315-005-045

2.0 mg315-005-008

1.5 mg315-005-046

2.0 mg 315-005-006

1.5 mg315-005-047

2.0 mg 315-005-044

1.5 mg315-005-048

2.0 mg315-005-020

Whole IgG ANTI-MOUSE1.5 mg

115-475-006

1.0 mg115-475-072

1.5 mg115-475-044

1.0 mg 115-475-068

1.5 mg115-475-020

1.0 mg 115-475-075

1.0 mg315-475-003

1.0 mg315-475-045

1.0 mg315-475-008

1.0 mg315-475-046

1.0 mg 315-475-006

1.0 mg315-475-047

1.0 mg 315-475-044

1.0 mg315-475-048

1.0 mg315-475-020

2.0 mg115-155-006

1.5 mg115-155-072

2.0 mg115-155-044

1.5 mg 115-155-068

2.0 mg115-155-020

1.0 mg 115-155-075

1.5 mg315-155-003

1.0 mg315-155-045

1.5 mg315-155-008

1.0 mg315-155-046

1.5 mg 315-155-006

1.0 mg315-155-047

1.5 mg 315-155-044

1.0 mg315-155-048

1.5 mg315-155-020

1.5 mg115-545-006

1.0 mg115-545-072

1.5 mg115-545-044

1.0 mg 115-545-068

1.5 mg115-545-020

1.0 mg 115-545-075

1.0 mg315-545-003

1.0 mg315-545-045

1.0 mg315-545-008

1.0 mg315-545-046

1.0 mg 315-545-006

1.0 mg315-545-047

1.0 mg 315-545-044

1.0 mg315-545-048

1.0 mg315-545-020




ML*

ML*

27



1.0 ml115-055-006

1.0 ml115-055-072

1.0 ml115-055-044

1.0 ml 115-055-068

1.0 ml115-055-020

1.0 ml 115-055-075

1.0 ml315-055-003

0.5 ml315-055-045

1.0 ml315-055-008

0.5 ml315-055-046

1.0 ml 315-055-006

0.5 ml315-055-047

1.0 ml315-055-044

0.5 ml315-055-048

1.0 ml315-055-020

2.0 ml115-065-006

1.5 ml115-065-072

2.0 ml115-065-044

1.5 ml115-065-068

2.0 ml115-065-020

1.0 ml115-065-075

1.5 ml315-065-003

1.0 ml315-065-045

1.5 ml315-065-008

1.0 ml315-065-046

1.5 ml315-065-006

1.0 ml315-065-047

1.5 ml315-065-044

1.0 ml315-065-048

1.5 ml315-065-020

2.0 ml115-035-006

1.5 ml115-035-072

2.0 ml115-035-044

1.5 ml115-035-068

2.0 ml115-035-020

1.0 ml 115-035-075

1.5 ml315-035-003

1.0 ml315-035-045

1.5 ml315-035-008

1.0 ml315-035-046

1.5 ml315-035-006

1.0 ml315-035-047

1.5 ml315-035-044

1.0 ml315-035-048

1.5 ml315-035-020

1.5 mg115-605-006

1.0 mg115-605-072

1.5 mg115-605-044

1.0 mg 115-605-068

1.5 mg115-605-020

1.0 mg 115-605-075

1.0 mg315-605-003

1.0 mg315-605-045

1.0 mg315-605-008

1.0 mg315-605-046

1.0 mg 315-605-006

1.0 mg315-605-047

1.0 mg 315-605-044

1.0 mg315-605-048

1.0 mg315-605-020

1.5 mg115-585-006

1.0 mg115-585-072

1.5 mg115-585-044

1.0 mg 115-585-068

1.5 mg115-585-020

1.0 mg 115-585-075

1.0 mg315-585-003

1.0 mg315-585-045

1.0 mg315-585-008

1.0 mg315-585-046

1.0 mg 315-585-006

1.0 mg315-585-047

1.0 mg 315-585-044

1.0 mg315-585-048

1.0 mg315-585-020

2.0 mg115-295-006

1.5 mg115-295-072

2.0 mg115-295-044

1.5 mg115-295-068

2.0 mg115-295-020

1.0 mg 115-295-075

1.5 mg315-295-003

1.0 mg315-295-045

1.5 mg315-295-008

1.0 mg315-295-046

1.5 mg 315-295-006

1.0 mg315-295-047

1.5 mg 315-295-044

1.0 mg315-295-048

1.5 mg315-295-020

2.0 mg115-165-006

1.5 mg115-165-072

2.0 mg115-165-044

1.5 mg 115-165-068

2.0 mg115-165-020

1.0 mg 115-165-075

1.5 mg315-165-003

1.0 mg315-165-045

1.5 mg315-165-008

1.0 mg315-165-046

1.5 mg315-165-006

1.0 mg315-165-047

1.5 mg 315-165-044

1.0 mg315-165-048

1.5 mg315-165-020

2.0 mg115-095-006

1.5 mg115-095-072

2.0 mg115-095-044

1.5 mg115-095-068

2.0 mg115-095-020

1.0 mg 115-095-075

1.5 mg315-095-003

1.0 mg315-095-045

1.5 mg315-095-008

1.0 mg315-095-046

1.5 mg 315-095-006

1.0 mg315-095-047

1.5 mg 315-095-044

1.0 mg315-095-048

1.5 mg315-095-020

AlkalinePhosphatase












1.5 mg315-005-049

1.0 mg415-005-166

2.0 mg 515-005-003

1.5 mg515-005-062

1.5 mg515-005-071

1.5 mg515-005-072

1.0 mg315-475-049

1.0 mg 415-475-166

1.5 mg515-475-003

1.0 mg515-475-062

1.0 mg515-475-071

1.0 mg515-475-072

1.0 mg315-155-049

1.0 mg415-155-166

2.0 mg 515-155-003

1.5 mg515-155-062

1.5 mg515-155-071

1.5 mg515-155-072

1.0 mg315-545-049

1.0 mg 415-545-166

1.5 mg515-545-003

1.0 mg515-545-062

1.0 mg515-545-071

1.0 mg515-545-072



Rabbit Anti-Mouse IgM, µ chain specific(min X Hu Sr Prot)

Rat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Rat Sr Prot)

Sheep Anti-Mouse IgG (H+L)

Sheep Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot)

Sheep Anti-Mouse IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)

Sheep Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)



Donkey Anti-Rabbit IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)

Goat Anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG (H+L)(min X Hu Sr Prot)

Goat Anti-Rabbit IgG (H+L)(min X Hu, Ms, Rat Sr Prot)

Goat Anti-Rabbit IgG, Fc fragment specific

Goat Anti-Rabbit IgG, Fc fragment specific(min X Hu Sr Prot)

Goat Anti-Rabbit IgG, F(ab')2 fragment specific

Goat Anti-Rabbit IgG, F(ab')2 fragment specific(min X Hu Sr Prot)

Whole IgG ANTI-RABBIT1.0 mg

711-005-152

2.0 mg111-005-003

1.5 mg111-005-045

1.5 mg111-005-144

2.0 mg111-005-008

1.5 mg111-005-046

2.0 mg111-005-006

1.5 mg111-005-047

0.5 mg711-475-152

1.5 mg 111-475-003

1.0 mg111-475-045

1.0 mg111-475-144

1.5 mg111-475-008

1.0 mg111-475-046

1.5 mg111-475-006

1.0 mg111-475-047

0.5 mg711-155-152

2.0 mg111-155-003

1.5 mg111-155-045

1.5 mg111-155-144

2.0 mg111-155-008

1.5 mg111-155-046

2.0 mg111-155-006

1.5 mg111-155-047

0.5 mg711-545-152

1.5 mg111-545-003

1.0 mg111-545-045

1.0 mg111-545-144

1.5 mg111-545-008

1.0 mg111-545-046

1.5 mg111-545-006

1.0 mg111-545-047

ML*

ML*

ML*

29



0.5 ml315-055-049

0.5 ml415-055-166

1.0 ml 515-055-003

1.0 ml515-055-062

1.0 ml515-055-071

1.0 ml515-055-072

1.0 ml315-065-049

1.0 ml415-065-166

2.0 ml 515-065-003

1.5 ml515-065-062

1.5 ml515-065-071

1.5 ml515-065-072

1.0 ml315-035-049

1.0 ml415-035-166

2.0 ml 515-035-003

1.5 ml515-035-062

1.5 ml515-035-071

1.5 ml515-035-072

1.0 mg315-605-049

1.0 mg 415-605-166

1.5 mg515-605-003

1.0 mg515-605-062

1.0 mg515-605-071

1.0 mg515-605-072

1.0 mg315-585-049

1.0 mg 415-585-166

1.5 mg515-585-003

1.0 mg515-585-062

1.0 mg515-585-071

1.0 mg515-585-072

1.0 mg315-295-049

1.0 mg415-295-166

2.0 mg 515-295-003

1.5 mg515-295-062

1.5 mg515-295-071

1.5 mg515-295-072

1.0 mg315-165-049

1.0 mg415-165-166

2.0 mg 515-165-003

1.5 mg515-165-062

1.5 mg515-165-071

1.5 mg515-165-072

1.0 mg315-095-049

1.0 mg415-095-166

2.0 mg 515-095-003

1.5 mg515-095-062

1.5 mg515-095-071

1.5 mg515-095-072

AlkalinePhosphatase








Whole IgG ANTI-RABBIT0.5 ml

711-055-152

1.0 ml111-055-003

1.0 ml111-055-045

1.0 ml111-055-144

1.0 ml111-055-008

1.0 ml111-055-046

1.0 ml111-055-006

1.0 ml111-055-047

0.5 ml711-065-152

2.0 ml111-065-003

1.5 ml111-065-045

1.5 ml111-065-144

2.0 ml111-065-008

1.5 ml111-065-046

2.0 ml111-065-006

1.5 ml111-065-047

0.5 ml711-035-152

2.0 ml111-035-003

1.5 ml111-035-045

1.5 ml111-035-144

2.0 ml111-035-008

1.5 ml111-035-046

2.0 ml111-035-006

1.5 ml111-035-047

0.5 mg711-605-152

1.5 mg111-605-003

1.0 mg111-605-045

1.0 mg111-605-144

1.5 mg111-605-008

1.0 mg111-605-046

1.5 mg111-605-006

1.0 mg111-605-047

0.5 mg711-585-152

1.5 mg 111-585-003

1.0 mg111-585-045

1.0 mg111-585-144

1.5 mg 111-585-008

1.0 mg111-585-046

1.5 mg 111-585-006

1.0 mg111-585-047

0.5 mg711-295-152

2.0 mg111-295-003

1.5 mg111-295-045

1.5 mg111-295-144

2.0 mg 111-295-008

1.5 mg111-295-046

2.0 mg 111-295-006

1.5 mg111-295-047

0.5 mg711-165-152

2.0 mg111-165-003

1.5 mg111-165-045

1.5 mg111-165-144

2.0 mg111-165-008

1.5 mg111-165-046

2.0 mg111-165-006

1.5 mg111-165-047

0.5 mg711-095-152

2.0 mg111-095-003

1.5 mg111-095-045

1.5 mg111-095-144

2.0 mg 111-095-008

1.5 mg111-095-046

2.0 mg 111-095-006

1.5 mg111-095-047


30 ** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).‡ This antibody reacts primarily with kappa light chains. It is not suitable for detection of primary antibodies with lambda light chains.



Donkey Anti-Rat IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

Donkey Anti-Rat IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)**

Goat Anti-Rat IgG (H+L)

Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Rb Sr Prot)

Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Ms, Rb Sr Prot)**

Goat Anti-Rat IgG, Light Chain Specific‡

(min X Bov, Gt, Hrs, Hu, Ms, Rb, Shp Ig)

Goat Anti-Rat IgG, Fcγ fragment specific

Goat Anti-Rat IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgG, F(ab')2 fragment specific

Goat Anti-Rat IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgG + IgM (H+L)

Whole IgG ANTI-RABBIT



Mouse Anti-Rabbit IgG (H+L)(min X Hu, Gt, Ms, Shp Sr Prot)

IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific (min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)

1.5 mg211-005-109

1.0 mg 211-002-171

1.0 mg211-475-109

1.0 mg211-155-109

1.0 mg211-545-109

0.5 mg211-542-171


1.0 mg712-005-150

1.0 mg712-005-153

2.0 mg112-005-003

1.5 mg112-005-062

1.5 mg112-005-143

1.0 mg112-005-167

1.0 mg 112-005-175

2.0 mg112-005-008

1.5 mg112-005-071

2.0 mg112-005-006

1.5 mg112-005-072

2.0 mg112-005-044

0.5 mg712-475-150

0.5 mg712-475-153

1.5 mg 112-475-003

1.0 mg112-475-062

1.0 mg112-475-143

0.5 mg112-475-167

1.5 mg112-475-008

1.0 mg112-475-071

1.5 mg112-475-006

1.0 mg112-475-072

1.5 mg112-475-044

0.5 mg712-155-150

0.5 mg712-155-153

2.0 mg112-155-003

1.5 mg112-155-062

1.5 mg112-155-143

0.5 mg112-155-167

2.0 mg112-155-008

1.5 mg112-155-071

2.0 mg112-155-006

1.5 mg112-155-072

2.0 mg112-155-044

0.5 mg712-545-150

0.5 mg712-545-153

1.5 mg112-545-003

1.0 mg112-545-062

1.0 mg112-545-143

0.5 mg112-545-167

0.5 mg 112-545-175

1.5 mg112-545-008

1.0 mg112-545-071

1.5 mg112-545-006

1.0 mg112-545-072

1.5 mg112-545-044

ML*

ML*

ML*

ML*

ML*

ML*

ML*

Whole IgG ANTI-RAT

31



AlkalinePhosphatase






0.5 ml211-055-109

0.5 ml 211-052-171

1.0 ml211-065-109

0.5 ml211-062-171

1.0 ml211-035-109

0.5 ml 211-032-171

1.0 mg211-605-109

0.5 mg211-602-171

1.0 mg211-585-109

0.5 mg211-582-171

1.0 mg211-295-109

1.0 mg211-165-109

0.5 mg211-162-171

1.0 mg211-095-109



0.5 ml712-055-150

0.5 ml712-055-153

1.0 ml112-055-003

1.0 ml112-055-062

1.0 ml112-055-143

0.5 ml112-055-167

0.5 ml 112-055-175

1.0 ml112-055-008

1.0 ml112-055-071

1.0 ml112-055-006

1.0 ml112-055-072

1.0 ml112-055-044

0.5 ml712-065-150

0.5 ml712-065-153

2.0 ml112-065-003

1.5 ml112-065-062

1.5 ml112-065-143

0.5 ml112-065-167

0.5 ml 112-065-175

2.0 ml112-065-008

1.5 ml112-065-071

2.0 ml112-065-006

1.5 ml112-065-072

2.0 ml112-065-044

0.5 ml712-035-150

0.5 ml712-035-153

2.0 ml112-035-003

1.5 ml112-035-062

1.5 ml112-035-143

0.5 ml112-035-167

0.5 ml 112-035-175

2.0 ml112-035-008

1.5 ml112-035-071

2.0 ml112-035-006

1.5 ml112-035-072

2.0 ml112-035-044

0.5 mg712-605-150

0.5 mg712-605-153

1.5 mg112-605-003

1.0 mg112-605-062

1.0 mg112-605-143

0.5 mg112-605-167

0.5 mg 112-605-175

1.5 mg112-605-008

1.0 mg112-605-071

1.5 mg112-605-006

1.0 mg112-605-072

1.5 mg112-605-044

0.5 mg712-585-150

0.5 mg712-585-153

1.5 mg112-585-003

1.0 mg112-585-062

1.0 mg112-585-143

0.5 mg112-585-167

0.5 mg 112-585-175

1.5 mg112-585-008

1.0 mg112-585-071

1.5 mg112-585-006

1.0 mg112-585-072

1.5 mg112-585-044

0.5 mg712-295-150

0.5 mg712-295-153

2.0 mg112-295-003

1.5 mg112-295-062

1.5 mg112-295-143

0.5 mg112-295-167

2.0 mg112-295-008

1.5 mg112-295-071

2.0 mg112-295-006

1.5 mg112-295-072

2.0 mg112-295-044

0.5 mg712-165-150

0.5 mg712-165-153

2.0 mg112-165-003

1.5 mg112-165-062

1.5 mg112-165-143

0.5 mg112-165-167

0.5 mg112-165-175

2.0 mg112-165-008

1.5 mg112-165-071

2.0 mg112-165-006

1.5 mg112-165-072

2.0 mg112-165-044

0.5 mg712-095-150

0.5 mg712-095-153

2.0 mg112-095-003

1.5 mg112-095-062

1.5 mg112-095-143

0.5 mg112-095-167

2.0 mg112-095-008

1.5 mg112-095-071

2.0 mg112-095-006

1.5 mg112-095-072

2.0 mg112-095-044

Whole IgG ANTI-RAT





Goat Anti-Rat IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgM, µ chain specific

Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot)

Mouse Anti-Rat IgG (H+L)(min X Ms Sr Prot)

Mouse Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot)

Mouse Anti-Rat IgG, Fcγ fragment specific(min X Hu, Bov, Hrs, Ms Sr Prot)

Mouse Anti-Rat IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs, Ms Sr Prot)

Rabbit Anti-Rat IgG (H+L)

Rabbit Anti-Rat IgG (H+L) (min X Hu Sr Prot)

Rabbit Anti-Rat IgG, Fcγ fragment specific(min X Hu Sr Prot)

Rabbit Anti-Rat IgG, F(ab')2 fragment specific(min X Hu Sr Prot)

Rabbit Anti-Rat IgG + IgM (H+L)

Rabbit Anti-Rat IgG + IgM (H+L)(min X Hu Sr Prot)

Rabbit Anti-Rat IgM, µ chain specific

Rabbit Anti-Rat IgM, µ chain specific(min X Hu Sr Prot)

1.5 mg112-005-068

2.0 mg112-005-020

1.0 mg112-005-075

2.0 mg212-005-082

1.5 mg212-005-168

1.5 mg212-005-104

1.5 mg212-005-106

2.0 mg312-005-003

1.5 mg312-005-045

1.5 mg312-005-046

1.5 mg312-005-047

2.0 mg312-005-044

1.5 mg312-005-048

2.0 mg312-005-020

1.5 mg312-005-049

Whole IgG ANTI-RAT1.0 mg

112-475-068

1.5 mg112-475-020

1.0 mg 112-475-075

1.0 mg212-475-082

1.0 mg212-475-168

1.0 mg212-475-104

1.0 mg212-475-106

1.0 mg312-475-003

1.0 mg312-475-045

1.0 mg312-475-046

1.0 mg312-475-047

1.0 mg312-475-044

1.0 mg312-475-048

1.0 mg312-475-020

1.0 mg312-475-049

1.5 mg112-155-068

2.0 mg112-155-020

1.0 mg112-155-075

1.5 mg212-155-082

1.0 mg212-155-168

1.0 mg212-155-104

1.0 mg212-155-106

1.5 mg312-155-003

1.0 mg312-155-045

1.0 mg312-155-046

1.0 mg312-155-047

1.5 mg312-155-044

1.0 mg312-155-048

1.5 mg312-155-020

1.0 mg312-155-049

1.0 mg112-545-068

1.5 mg112-545-020

1.0 mg 112-545-075

1.0 mg212-545-082

1.0 mg212-545-168

1.0 mg212-545-104

1.0 mg212-545-106

1.0 mg312-545-003

1.0 mg312-545-045

1.0 mg312-545-046

1.0 mg312-545-047

1.0 mg312-545-044

1.0 mg312-545-048

1.0 mg312-545-020

1.0 mg312-545-049




ML*

ML*

ML*

33



1.0 ml112-055-068

1.0 ml112-055-020

1.0 ml112-055-075

1.0 ml212-055-082

0.5 ml212-055-168

0.5 ml212-055-104

0.5 ml212-055-106

1.0 ml312-055-003

0.5 ml312-055-045

0.5 ml312-055-046

0.5 ml312-055-047

1.0 ml312-055-044

0.5 ml312-055-048

1.0 ml312-055-020

0.5 ml312-055-049

1.5 ml112-065-068

2.0 ml112-065-020

1.0 ml112-065-075

1.5 ml212-065-082

1.0 ml212-065-168

1.0 ml212-065-104

1.0 ml212-065-106

1.5 ml312-065-003

1.0 ml312-065-045

1.0 ml312-065-046

1.0 ml312-065-047

1.5 ml312-065-044

1.0 ml312-065-048

1.5 ml312-065-020

1.0 ml312-065-049

1.5 ml112-035-068

2.0 ml112-035-020

1.0 ml112-035-075

1.5 ml212-035-082

1.0 ml212-035-168

1.0 ml212-035-104

1.0 ml212-035-106

1.5 ml312-035-003

1.0 ml312-035-045

1.0 ml312-035-046

1.0 ml312-035-047

1.5 ml312-035-044

1.0 ml312-035-048

1.5 ml312-035-020

1.0 ml312-035-049

1.0 mg112-605-068

1.5 mg112-605-020

1.0 mg 112-605-075

1.0 mg212-605-082

1.0 mg212-605-168

1.0 mg212-605-104

1.0 mg212-605-106

1.0 mg312-605-003

1.0 mg312-605-045

1.0 mg312-605-046

1.0 mg312-605-047

1.0 mg312-605-044

1.0 mg312-605-048

1.0 mg312-605-020

1.0 mg312-605-049

1.0 mg112-585-068

1.5 mg112-585-020

1.0 mg 112-585-075

1.0 mg212-585-082

1.0 mg212-585-168

1.0 mg212-585-104

1.0 mg212-585-106

1.0 mg312-585-003

1.0 mg312-585-045

1.0 mg312-585-046

1.0 mg312-585-047

1.0 mg312-585-044

1.0 mg312-585-048

1.0 mg312-585-020

1.0 mg312-585-049

1.5 mg112-295-068

2.0 mg112-295-020

1.0 mg112-295-075

1.5 mg212-295-082

1.0 mg212-295-168

1.0 mg212-295-104

1.0 mg212-295-106

1.5 mg312-295-003

1.0 mg312-295-045

1.0 mg312-295-046

1.0 mg312-295-047

1.5 mg312-295-044

1.0 mg312-295-048

1.5 mg312-295-020

1.0 mg312-295-049

1.5 mg112-165-068

2.0 mg112-165-020

1.0 mg112-165-075

1.5 mg212-165-082

1.0 mg212-165-168

1.0 mg212-165-104

1.0 mg212-165-106

1.5 mg312-165-003

1.0 mg312-165-045

1.0 mg312-165-046

1.0 mg312-165-047

1.5 mg312-165-044

1.0 mg312-165-048

1.5 mg312-165-020

1.0 mg312-165-049

1.5 mg112-095-068

2.0 mg112-095-020

1.0 mg112-095-075

1.5 mg212-095-082

1.0 mg212-095-168

1.0 mg212-095-104

1.0 mg212-095-106

1.5 mg312-095-003

1.0 mg312-095-045

1.0 mg312-095-046

1.0 mg312-095-047

1.5 mg312-095-044

1.0 mg312-095-048

1.5 mg312-095-020

1.0 mg312-095-049

AlkalinePhosphatase









34 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).



Donkey Anti-Sheep IgG (H+L)***

Donkey Anti-Sheep IgG (H+L)***(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

IgG Fraction Monoclonal Mouse Anti-Sheep IgG, Light Chain Specific(min X Bov, Hrs, Hu, Ms, Rb, Rat Ig)

Rabbit Anti-Sheep IgG (H+L)***

Rabbit Anti-Sheep IgG (H+L)***(min X Hu Sr Prot)

Rabbit Anti-Sheep IgG, Fc fragment specific***(min X Hu Sr Prot)

Rabbit Anti-Sheep IgG, F(ab')2 fragment specific***(min X Hu Sr Prot)

Goat Anti-Swine IgG (H+L)

Whole IgG ANTI-SHEEP




1.0 mg713-005-003

1.0 mg713-005-147

1.0 mg213-002-177

2.0 mg313-005-003

1.5 mg313-005-045

1.5 mg313-005-046

1.5 mg313-005-047

2.0 mg114-005-003

1.0 mg713-475-003

0.5 mg713-475-147

1.0 mg313-475-003

1.0 mg313-475-045

1.0 mg313-475-046

1.0 mg313-475-047

1.5 mg114-475-003

1.0 mg713-155-003

0.5 mg713-155-147

1.5 mg313-155-003

1.0 mg313-155-045

1.0 mg313-155-046

1.0 mg313-155-047

2.0 mg114-155-003

1.0 mg713-545-003

0.5 mg713-545-147

0.5 mg 213-542-177

1.0 mg313-545-003

1.0 mg313-545-045

1.0 mg313-545-046

1.0 mg313-545-047

1.5 mg114-545-003

Whole IgG ANTI-SWINE

ML*

35



AlkalinePhosphatase








1.0 ml713-055-003

0.5 ml713-055-147

0.5 ml 213-052-177

1.0 ml313-055-003

0.5 ml313-055-045

0.5 ml313-055-046

0.5 ml313-055-047

1.0 ml114-055-003

1.0 ml713-065-003

0.5 ml713-065-147

0.5 ml 213-062-177

1.5 ml313-065-003

1.0 ml313-065-045

1.0 ml313-065-046

1.0 ml313-065-047

2.0 ml114-065-003

1.0 ml713-035-003

0.5 ml713-035-147

0.5 ml 213-032-177

1.5 ml313-035-003

1.0 ml313-035-045

1.0 ml313-035-046

1.0 ml313-035-047

2.0 ml114-035-003

1.0 mg713-605-003

0.5 mg713-605-147

0.5 mg213-602-177

1.0 mg313-605-003

1.0 mg313-605-045

1.0 mg313-605-046

1.0 mg313-605-047

1.5 mg114-605-003

1.0 mg713-585-003

0.5 mg713-585-147

0.5 mg213-582-177

1.0 mg313-585-003

1.0 mg313-585-045

1.0 mg313-585-046

1.0 mg313-585-047

1.5 mg114-585-003

1.0 mg713-295-003

0.5 mg713-295-147

1.5 mg313-295-003

1.0 mg313-295-045

1.0 mg313-295-046

1.0 mg313-295-047

2.0 mg114-295-003

1.0 mg713-165-003

0.5 mg713-165-147

0.5 mg 213-162-177

1.5 mg313-165-003

1.0 mg313-165-045

1.0 mg313-165-046

1.0 mg313-165-047

2.0 mg114-165-003

1.0 mg713-095-003

0.5 mg713-095-147

1.5 mg313-095-003

1.0 mg313-095-045

1.0 mg313-095-046

1.0 mg313-095-047

2.0 mg114-095-003

Whole IgG ANTI-SWINE


36 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

F(ab')2 Fragment Affinity-Purified Antibodies


Goat Anti-Bovine IgG (H+L)***

Donkey Anti-Chicken IgY† (IgG) (H+L)(min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Rabbit Anti-Chicken IgY† (IgG) (H+L)

Donkey Anti-Goat IgG (H+L)***(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)


Rabbit Anti-Goat IgG (H+L)***(min X Hu Sr Prot)

Rabbit Anti-Goat IgG, Fc fragment specific***

Rabbit Anti-Goat IgG, Fc fragment specific***(min X Hu Sr Prot)

Rabbit Anti-Goat IgG, F(ab')2 fragment specific***

Rabbit Anti-Goat IgG, F(ab')2 fragment specific***(min X Hu Sr Prot)

Donkey Anti-Guinea Pig IgG (H+L)(min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Goat Anti-Guinea Pig IgG (H+L)

F(ab')2 Fragment ANTI-BOVINE

Unconjugated Coumarin AMCAA=350, E=450

F(ab')2 Fragment ANTI-CHICKEN

F(ab')2 Fragment ANTI-GOAT

F(ab')2 Fragment ANTI-GUINEA PIG


1.0 mg101-006-003

0.5 mg703-006-155

1.0 mg303-006-003

0.5 mg705-006-147

1.0 mg305-006-003

0.5 mg305-006-045

1.0 mg305-006-008

0.5 mg305-006-046

1.0 mg305-006-006

0.5 mg305-006-047

0.5 mg706-006-148

1.0 mg106-006-003

1.0 mg101-156-003

0.3 mg703-156-155

0.5 mg303-156-003

0.3 mg705-156-147

0.5 mg305-156-003

0.5 mg305-156-045

0.5 mg305-156-008

0.5 mg305-156-046

0.5 mg305-156-006

0.5 mg305-156-047

0.3 mg706-156-148

1.0 mg106-156-003

0.75 mg101-546-003

0.3 mg703-546-155

0.5 mg303-546-003

0.3 mg705-546-147

0.5 mg305-546-003

0.5 mg305-546-045

0.5 mg305-546-008

0.5 mg305-546-046

0.5 mg305-546-006

0.5 mg305-546-047

0.3 mg706-546-148

0.75 mg 106-546-003

ML*

ML*

ML*

37



AlkalinePhosphatase








0.5 ml101-056-003

0.3 ml703-056-155

0.5 ml303-056-003

0.3 ml705-056-147

0.5 ml305-056-003

0.5 ml305-056-045

0.5 ml305-056-008

0.5 ml305-056-046

0.5 ml305-056-006

0.5 ml305-056-047

0.3 ml706-056-148

0.5 ml106-056-003

0.5 ml101-066-003

0.3 ml703-066-155

0.5 ml303-066-003

0.3 ml705-066-147

0.5 ml305-066-003

0.5 ml305-066-045

0.5 ml305-066-008

0.5 ml305-066-046

0.5 ml305-066-006

0.5 ml305-066-047

0.3 ml706-066-148

0.5 ml106-066-003

0.5 ml101-036-003

0.3 ml703-036-155

0.5 ml303-036-003

0.3 ml705-036-147

0.5 ml305-036-003

0.5 ml305-036-045

0.5 ml305-036-008

0.5 ml305-036-046

0.5 ml305-036-006

0.5 ml305-036-047

0.3 ml706-036-148

0.5 ml106-036-003

1.0 mg101-096-003

0.3 mg703-096-155

0.5 mg303-096-003

0.3 mg705-096-147

0.5 mg305-096-003

0.5 mg305-096-045

0.5 mg305-096-008

0.5 mg305-096-046

0.5 mg305-096-006

0.5 mg305-096-047

0.3 mg706-096-148

1.0 mg106-096-003

0.75 mg101-606-003

0.3 mg703-606-155

0.5 mg303-606-003

0.3 mg705-606-147

0.5 mg305-606-003

0.5 mg305-606-045

0.5 mg305-606-008

0.5 mg305-606-046

0.5 mg305-606-006

0.5 mg305-606-047

0.3 mg706-606-148

0.75 mg 106-606-003

0.75 mg101-586-003

0.3 mg703-586-155

0.5 mg303-586-003

0.3 mg705-586-147

0.5 mg305-586-003

0.5 mg305-586-045

0.5 mg305-586-008

0.5 mg305-586-046

0.5 mg305-586-006

0.5 mg305-586-047

0.3 mg706-586-148

0.75 mg 106-586-003

1.0 mg101-296-003

0.3 mg703-296-155

0.5 mg303-296-003

0.3 mg705-296-147

0.5 mg305-296-003

0.5 mg305-296-045

0.5 mg305-296-008

0.5 mg305-296-046

0.5 mg305-296-006

0.5 mg305-296-047

0.3 mg706-296-148

1.0 mg106-296-003

1.0 mg101-166-003

0.3 mg703-166-155

0.5 mg303-166-003

0.3 mg705-166-147

0.5 mg305-166-003

0.5 mg305-166-045

0.5 mg305-166-008

0.5 mg305-166-046

0.5 mg305-166-006

0.5 mg305-166-047

0.3 mg706-166-148

1.0 mg106-166-003





Goat Anti-Syrian Hamster IgG (H+L)(min X Bov, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Rabbit Anti-Syrian Hamster IgG (H+L)

Goat Anti-Horse IgG (H+L)


Donkey Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot)Donkey Anti-Human IgM, Fc5µ fragment specific(min X Bov, Hrs Sr Prot)



Goat Anti-Human IgG, Fcγ fragment specific


Goat Anti-Human IgG, F(ab')2 fragment specific

Goat Anti-Human IgG, F(ab')2 fragment specific(min X Bov, Hrs, Ms Sr Prot)

Goat Anti-Human IgG + IgM (H+L)(min X Bov Sr Prot)

Goat Anti-Human IgA + IgG + IgM (H+L)

F(ab')2 Fragment ANTI-HAMSTER (Syrian Hamster)


F(ab')2 Fragment ANTI-HUMAN


0.5 mg107-156-142

0.5 mg307-156-003

1.0 mg108-156-003

0.3 mg709-156-149

1.0 mg 709-156-098

1.0 mg709-156-073

1.0 mg 109-156-003

1.0 mg109-156-088

1.0 mg109-156-008

1.0 mg109-156-098

1.0 mg109-156-006

1.0 mg109-156-097

1.0 mg109-156-127

1.0 mg109-156-064

0.5 mg107-546-142

0.5 mg307-546-003

0.75 mg108-546-003

0.3 mg709-546-149

0.75 mg 709-546-098

0.75 mg 709-546-073

0.75 mg 109-546-003

0.75 mg 109-546-088

0.75 mg 109-546-008

0.75 mg109-546-098

0.75 mg 109-546-006

0.75 mg 109-546-097

0.75 mg 109-546-127

0.75 mg 109-546-064

F(ab')2 Fragment ANTI-HORSE

ML*

ML*

0.5 mg107-006-142

1.0 mg307-006-003

1.0 mg108-006-003

0.5 mg709-006-149

1.0 mg709-006-098

1.0 mg709-006-073

1.0 mg 109-006-003

1.0 mg109-006-088

1.0 mg109-006-008

1.0 mg109-006-098

1.0 mg109-006-006

1.0 mg109-006-097

1.0 mg109-006-127

1.0 mg109-006-064

39



AlkalinePhosphatase








0.5 ml107-056-142

0.5 ml307-056-003

0.5 ml108-056-003

0.3 ml709-056-149

0.5 ml709-056-098

0.5 ml709-056-073

0.5 ml109-056-003

0.5 ml109-056-088

0.5 ml109-056-008

0.5 ml109-056-098

0.5 ml109-056-006

0.5 ml109-056-097

0.5 ml109-056-127

0.5 ml109-056-064

0.5 ml107-066-142

0.5 ml307-066-003

0.5 ml108-066-003

0.3 ml709-066-149

0.5 ml 709-066-098

0.5 ml709-066-073

0.5 ml 109-066-003

0.5 ml109-066-088

0.5 ml109-066-008

0.5 ml 109-066-098

0.5 ml109-066-006

0.5 ml109-066-097

0.5 ml109-066-127

0.5 ml109-066-064

0.5 ml107-036-142

0.5 ml307-036-003

0.5 ml108-036-003

0.3 ml709-036-149

0.5 ml 709-036-098

0.5 ml709-036-073

0.5 ml109-036-003

0.5 ml109-036-088

0.5 ml109-036-008

0.5 ml109-036-098

0.5 ml109-036-006

0.5 ml109-036-097

0.5 ml109-036-127

0.5 ml109-036-064

0.5 mg107-606-142

0.5 mg307-606-003

0.75 mg108-606-003

0.3 mg709-606-149

0.75 mg709-606-098

0.75 mg 709-606-073

0.75 mg 109-606-003

0.75 mg 109-606-088

0.75 mg 109-606-008

0.75 mg 109-606-098

0.75 mg109-606-006

0.75 mg 109-606-097

0.75 mg 109-606-127

0.75 mg 109-606-064

0.5 mg107-586-142

0.5 mg307-586-003

0.75 mg108-586-003

0.3 mg709-586-149

0.75 mg 709-586-098

0.75 mg 709-586-073

0.75 mg 109-586-003

0.75 mg 109-586-088

0.75 mg 109-586-008

0.75 mg 109-586-098

0.75 mg 109-586-006

0.75 mg 109-586-097

0.75 mg 109-586-127

0.75 mg 109-586-064

0.5 mg107-296-142

0.5 mg307-296-003

1.0 mg108-296-003

0.3 mg709-296-149

1.0 mg 709-296-098

1.0 mg709-296-073

1.0 mg 109-296-003

1.0 mg109-296-088

1.0 mg109-296-008

1.0 mg109-296-098

1.0 mg109-296-006

1.0 mg109-296-097

1.0 mg109-296-127

1.0 mg109-296-064

0.5 mg107-166-142

0.5 mg307-166-003

1.0 mg108-166-003

0.3 mg709-166-149

1.0 mg 709-166-098

1.0 mg709-166-073

1.0 mg 109-166-003

1.0 mg109-166-088

1.0 mg109-166-008

1.0 mg109-166-098

1.0 mg109-166-006

1.0 mg109-166-097

1.0 mg109-166-127

1.0 mg109-166-064

0.5 mg107-096-142

0.5 mg307-096-003

1.0 mg108-096-003

0.3 mg709-096-149

1.0 mg 709-096-098

1.0 mg709-096-073

1.0 mg 109-096-003

1.0 mg109-096-088

1.0 mg109-096-008

1.0 mg109-096-098

1.0 mg109-096-006

1.0 mg109-096-097

1.0 mg109-096-127

1.0 mg109-096-064

F(ab')2 Fragment ANTI-HORSE





Goat Anti-Human IgM, Fc5µ fragment specific (min X Bov Sr Prot)


Rabbit Anti-Human IgG (H+L)

Rabbit Anti-Human IgG, Fcγ fragment specific

Rabbit Anti-Human IgM, Fc5µ fragment specific

F(ab')2 Fragment ANTI-HUMAN1.0 mg

109-006-129

1.0 mg109-006-011

1.0 mg 309-006-003

1.0 mg 309-006-008

1.0 mg309-006-043

1.0 mg109-156-129

1.0 mg109-156-011

0.5 mg309-156-003

0.5 mg309-156-008

0.5 mg309-156-043

0.75 mg109-546-129

0.75 mg 109-546-011

0.5 mg 309-546-003

0.5 mg 309-546-008

0.5 mg309-546-043


F(ab')2 Fragment ANTI-MOUSEDonkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

Donkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)**

Donkey Anti-Mouse IgM, µ chain specific


Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)

Goat Anti-Mouse IgG, Fcγ fragment specific


Goat Anti-Mouse IgG, F(ab')2 fragment specific


0.5 mg715-006-150

0.5 mg715-006-151

1.0 mg715-006-020

1.0 mg115-006-003

1.0 mg115-006-062

1.0 mg115-006-146

1.0 mg115-006-008

1.0 mg115-006-071

1.0 mg115-006-006

0.3 mg715-156-150

0.3 mg715-156-151

1.0 mg715-156-020

1.0 mg115-156-003

1.0 mg115-156-062

1.0 mg115-156-146

1.0 mg115-156-008

1.0 mg115-156-071

1.0 mg115-156-006

0.3 mg715-546-150

0.3 mg715-546-151

0.75 mg 715-546-020

0.75 mg 115-546-003

0.75 mg 115-546-062

0.75 mg 115-546-146

0.75 mg 115-546-008

0.75 mg 115-546-071

0.75 mg115-546-006

ML*

ML*

ML*

ML*

ML*

41



AlkalinePhosphatase








0.5 ml109-056-129

0.5 ml109-056-011

0.5 ml 309-056-003

0.5 ml 309-056-008

0.5 ml309-056-043

0.5 ml109-066-129

0.5 ml109-066-011

0.5 ml 309-066-003

0.5 ml 309-066-008

0.5 ml309-066-043

0.5 ml109-036-129

0.5 ml109-036-011

0.5 ml309-036-003

0.5 ml 309-036-008

0.5 ml309-036-043

0.75 mg109-606-129

0.75 mg 109-606-011

0.5 mg 309-606-003

0.5 mg 309-606-008

0.5 mg309-606-043

0.75 mg109-586-129

0.75 mg 109-586-011

0.5 mg 309-586-003

0.5 mg 309-586-008

0.5 mg309-586-043

1.0 mg109-296-129

1.0 mg109-296-011

0.5 mg 309-296-003

0.5 mg 309-296-008

0.5 mg309-296-043

1.0 mg109-166-129

1.0 mg109-166-011

0.5 mg 309-166-003

0.5 mg 309-166-008

0.5 mg309-166-043

1.0 mg109-096-129

1.0 mg109-096-011

0.5 mg309-096-003

0.5 mg 309-096-008

0.5 mg309-096-043

0.3 ml715-056-150

0.3 ml715-056-151

0.5 ml715-056-020

0.5 ml115-056-003

0.5 ml115-056-062

0.5 ml115-056-146

0.5 ml115-056-008

0.5 ml115-056-071

0.5 ml115-056-006

0.3 ml715-066-150

0.3 ml715-066-151

0.5 ml715-066-020

0.5 ml115-066-003

0.5 ml115-066-062

0.5 ml115-066-146

0.5 ml115-066-008

0.5 ml115-066-071

0.5 ml115-066-006

0.3 ml715-036-150

0.3 ml715-036-151

0.5 ml715-036-020

0.5 ml115-036-003

0.5 ml115-036-062

0.5 ml115-036-146

0.5 ml115-036-008

0.5 ml115-036-071

0.5 ml115-036-006

0.3 mg715-606-150

0.3 mg715-606-151

0.75 mg 715-606-020

0.75 mg 115-606-003

0.75 mg 115-606-062

0.75 mg 115-606-146

0.75 mg 115-606-008

0.75 mg 115-606-071

0.75 mg115-606-006

0.3 mg715-586-150

0.3 mg715-586-151

0.75 mg 715-586-020

0.75 mg 115-586-003

0.75 mg 115-586-062

0.75 mg 115-586-146

0.75 mg 115-586-008

0.75 mg 115-586-071

0.75 mg115-586-006

0.3 mg715-296-150

0.3 mg715-296-151

1.0 mg715-296-020

1.0 mg115-296-003

1.0 mg115-296-062

1.0 mg115-296-146

1.0 mg115-296-008

1.0 mg115-296-071

1.0 mg115-296-006

0.3 mg715-166-150

0.3 mg715-166-151

1.0 mg715-166-020

1.0 mg115-166-003

1.0 mg115-166-062

1.0 mg115-166-146

1.0 mg115-166-008

1.0 mg115-166-071

1.0 mg115-166-006

0.3 mg715-096-150

0.3 mg715-096-151

1.0 mg715-096-020

1.0 mg115-096-003

1.0 mg115-096-062

1.0 mg115-096-146

1.0 mg115-096-008

1.0 mg115-096-071

1.0 mg115-096-006





Goat Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgM, µ chain specific



Rabbit Anti-Mouse IgG (H+L)(min X Hu Sr Prot)

Rabbit Anti-Mouse IgG, Fcγ fragment specific(min X Hu Sr Prot)

Rabbit Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu Sr Prot)

F(ab')2 Fragment ANTI-MOUSE1.0 mg

115-006-072

1.0 mg115-006-068

1.0 mg115-006-020

1.0 mg115-006-075

1.0 mg 315-006-003

0.5 mg 315-006-045

0.5 mg315-006-046

0.5 mg315-006-047

1.0 mg115-156-072

1.0 mg115-156-068

1.0 mg115-156-020

1.0 mg115-156-075

0.5 mg 315-156-003

0.5 mg 315-156-045

0.5 mg315-156-046

0.5 mg315-156-047

0.75 mg 115-546-072

0.75 mg 115-546-068

0.75 mg 115-546-020

0.75 mg 115-546-075

0.5 mg 315-546-003

0.5 mg 315-546-045

0.5 mg315-546-046

0.5 mg315-546-047




Goat Anti-Rabbit IgG (H+L)(min X Hu Sr Prot)


Goat Anti-Rabbit IgG, Fc fragment specific(min X Hu Sr Prot)

Goat Anti-Rabbit IgG, F(ab')2 fragment specific(min X Hu Sr Prot)

0.5 mg711-006-152

1.0 mg111-006-003

1.0 mg111-006-045

1.0 mg111-006-144

1.0 mg111-006-046

1.0 mg111-006-047

0.3 mg711-156-152

1.0 mg111-156-003

1.0 mg111-156-045

1.0 mg111-156-144

1.0 mg111-156-046

1.0 mg111-156-047

0.3 mg711-546-152

0.75 mg 111-546-003

0.75 mg 111-546-045

0.75 mg 111-546-144

0.75 mg 111-546-046

0.75 mg 111-546-047

F(ab')2 Fragment ANTI-RABBIT


ML*

ML*

ML*

ML*

43



AlkalinePhosphatase








0.5 ml115-056-072

0.5 ml115-056-068

0.5 ml115-056-020

0.5 ml115-056-075

0.5 ml 315-056-003

0.5 ml315-056-045

0.5 ml315-056-046

0.5 ml315-056-047

0.5 ml115-066-072

0.5 ml115-066-068

0.5 ml115-066-020

0.5 ml115-066-075

0.5 ml 315-066-003

0.5 ml 315-066-045

0.5 ml315-066-046

0.5 ml315-066-047

0.5 ml115-036-072

0.5 ml115-036-068

0.5 ml115-036-020

0.5 ml115-036-075

0.5 ml 315-036-003

0.5 ml 315-036-045

0.5 ml315-036-046

0.5 ml315-036-047

0.75 mg 115-606-072

0.75 mg 115-606-068

0.75 mg 115-606-020

0.75 mg 115-606-075

0.5 mg 315-606-003

0.5 mg 315-606-045

0.5 mg315-606-046

0.5 mg315-606-047

0.75 mg 115-586-072

0.75 mg 115-586-068

0.75 mg 115-586-020

0.75 mg 115-586-075

0.5 mg 315-586-003

0.5 mg 315-586-045

0.5 mg315-586-046

0.5 mg315-586-047

1.0 mg115-296-072

1.0 mg115-296-068

1.0 mg115-296-020

1.0 mg115-296-075

0.5 mg 315-296-003

0.5 mg 315-296-045

0.5 mg315-296-046

0.5 mg315-296-047

1.0 mg115-166-072

1.0 mg115-166-068

1.0 mg115-166-020

1.0 mg115-166-075

0.5 mg 315-166-003

0.5 mg315-166-045

0.5 mg315-166-046

0.5 mg315-166-047

1.0 mg115-096-072

1.0 mg115-096-068

1.0 mg115-096-020

1.0 mg115-096-075

0.5 mg 315-096-003

0.5 mg 315-096-045

0.5 mg315-096-046

0.5 mg315-096-047

0.3 ml711-056-152

0.5 ml111-056-003

0.5 ml111-056-045

0.5 ml111-056-144

0.5 ml111-056-046

0.5 ml111-056-047

0.3 ml711-066-152

0.5 ml111-066-003

0.5 ml111-066-045

0.5 ml111-066-144

0.5 ml111-066-046

0.5 ml111-066-047

0.3 ml711-036-152

0.5 ml111-036-003

0.5 ml111-036-045

0.5 ml111-036-144

0.5 ml111-036-046

0.5 ml111-036-047

0.3 mg711-606-152

0.75 mg 111-606-003

0.75 mg 111-606-045

0.75 mg 111-606-144

0.75 mg 111-606-046

0.75 mg 111-606-047

0.3 mg711-586-152

0.75 mg 111-586-003

0.75 mg 111-586-045

0.75 mg 111-586-144

0.75 mg 111-586-046

0.75 mg111-586-047

0.3 mg711-296-152

1.0 mg111-296-003

1.0 mg111-296-045

1.0 mg111-296-144

1.0 mg111-296-046

1.0 mg111-296-047

0.3 mg711-166-152

1.0 mg111-166-003

1.0 mg111-166-045

1.0 mg111-166-144

1.0 mg111-166-046

1.0 mg111-166-047

0.3 mg711-096-152

1.0 mg111-096-003

1.0 mg111-096-045

1.0 mg111-096-144

1.0 mg111-096-046

1.0 mg111-096-047




Antibody Description Unconjugated Coumarin AMCAA=350, E=450

F(ab')2 Fragment ANTI-RATDonkey Anti-Rat IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)



Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs Sr Prot)



Goat Anti-Rat IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgM, µ chain specific(min X Hu, Bov, Hrs Sr Prot)

Mouse Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot)

Rabbit Anti-Rat IgG (H+L)(min X Hu Sr Prot)




0.3 mg712-156-150

0.3 mg712-156-153

1.0 mg112-156-003

1.0 mg112-156-062

1.0 mg112-156-143

1.0 mg112-156-071

1.0 mg112-156-072

1.0 mg112-156-068

1.0 mg112-156-075

0.5 mg212-156-168

0.5 mg312-156-045

0.3 mg713-156-147

0.5 mg313-156-003

0.3 mg712-546-150

0.3 mg712-546-153

0.75 mg112-546-003

0.75 mg112-546-062

0.75 mg112-546-143

0.75 mg112-546-071

0.75 mg112-546-072

0.75 mg112-546-068

0.75 mg112-546-075

0.5 mg212-546-168

0.5 mg312-546-045

0.3 mg713-546-147

0.5 mg313-546-003

F(ab')2 Fragment ANTI-SHEEP

ML*

ML*

ML*

ML*

ML*

ML*

ML*

0.5 mg712-006-150

0.5 mg712-006-153

1.0 mg112-006-003

1.0 mg112-006-062

1.0 mg112-006-143

1.0 mg112-006-071

1.0 mg112-006-072

1.0 mg112-006-068

1.0 mg112-006-075

0.5 mg212-006-168

0.5 mg312-006-045

0.5 mg713-006-147

1.0 mg313-006-003

45



AlkalinePhosphatase








0.3 ml712-056-150

0.3 ml712-056-153

0.5 ml112-056-003

0.5 ml112-056-062

0.5 ml112-056-143

0.5 ml112-056-071

0.5 ml112-056-072

0.5 ml112-056-068

0.5 ml112-056-075

0.5 ml212-056-168

0.5 ml312-056-045

0.3 ml713-056-147

0.5 ml313-056-003

0.3 ml712-066-150

0.3 ml712-066-153

0.5 ml112-066-003

0.5 ml112-066-062

0.5 ml112-066-143

0.5 ml112-066-071

0.5 ml112-066-072

0.5 ml112-066-068

0.5 ml112-066-075

0.5 ml 212-066-168

0.5 ml312-066-045

0.3 ml713-066-147

0.5 ml313-066-003

0.3 ml712-036-150

0.3 ml712-036-153

0.5 ml112-036-003

0.5 ml112-036-062

0.5 ml112-036-143

0.5 ml112-036-071

0.5 ml112-036-072

0.5 ml112-036-068

0.5 ml112-036-075

0.5 ml 212-036-168

0.5 ml312-036-045

0.3 ml713-036-147

0.5 ml313-036-003

0.3 mg712-606-150

0.3 mg712-606-153

0.75 mg 112-606-003

0.75 mg 112-606-062

0.75 mg 112-606-143

0.75 mg 112-606-071

0.75 mg112-606-072

0.75 mg 112-606-068

0.75 mg 112-606-075

0.5 mg 212-606-168

0.5 mg312-606-045

0.3 mg713-606-147

0.5 mg313-606-003

0.3 mg712-586-150

0.3 mg712-586-153

0.75 mg 112-586-003

0.75 mg 112-586-062

0.75 mg 112-586-143

0.75 mg 112-586-071

0.75 mg112-586-072

0.75 mg 112-586-068

0.75 mg 112-586-075

0.5 mg 212-586-168

0.5 mg312-586-045

0.3 mg713-586-147

0.5 mg313-586-003

0.3 mg712-296-150

0.3 mg712-296-153

1.0 mg112-296-003

1.0 mg112-296-062

1.0 mg112-296-143

1.0 mg112-296-071

1.0 mg112-296-072

1.0 mg112-296-068

1.0 mg112-296-075

0.5 mg 212-296-168

0.5 mg312-296-045

0.3 mg713-296-147

0.5 mg313-296-003

0.3 mg712-166-150

0.3 mg712-166-153

1.0 mg112-166-003

1.0 mg112-166-062

1.0 mg112-166-143

1.0 mg112-166-071

1.0 mg112-166-072

1.0 mg112-166-068

1.0 mg112-166-075

0.5 mg 212-166-168

0.5 mg312-166-045

0.3 mg713-166-147

0.5 mg313-166-003

0.3 mg712-096-150

0.3 mg712-096-153

1.0 mg112-096-003

1.0 mg112-096-062

1.0 mg112-096-143

1.0 mg112-096-071

1.0 mg112-096-072

1.0 mg112-096-068

1.0 mg112-096-075

0.5 mg 212-096-168

0.5 mg312-096-045

0.3 mg713-096-147

0.5 mg313-096-003


Monovalent Fab fragments of affinity-purified secondary antibodies are offered to cover(block) the surface of immunoglobulins for double labeling primary antibodies from thesame host species, or to block endogenous immunoglobulins in tissue sections or on cellsurfaces. They can be used for these purposes because Fab fragments have a singleantigen binding site (i.e. they are monovalent).

In contrast, divalent antibodies (whole IgG and F(ab')2 fragments) have two antigen bindingsites. After labeling the first primary antibody, some antigen binding sites on the firstsecondary antibody may remain open which could capture the second primary antibodyintroduced in a subsequent step. Consequently, it will appear as overlapping labeling, eventhough there may not be overlapping antigens. Therefore, divalent antibodies should notbe used for blocking or for double labeling two primary antibodies from the same species.For selected literature references see Wessel and McClay, J. Histochem. Cytochem. 1986.34, 703; Franzusoff et al., J. Cell Biology 1991. 112, 27; Lewis Carl et al., J. Histochem.Cytochem. 1993. 41, 1273; and Negoescu et al., J. Histochem. Cytochem. 1994. 42, 433.

Monovalent Fab secondary antibodies are not necessary when primary antibodies fromthe same host species are different classes of immunoglobulins, such as IgG and IgM; ordifferent subclasses of IgG, such as Mouse IgG1 and Mouse IgG2a. In these cases, it ismuch easier and more effective to use class-specific or subclass-specific antibodies,respectively, to distinguish between the two primary antibodies from the same species.

To block endogenous immunoglobulins on cells or tissue sections, incubate with an excess(20-40 μg/ml) of unconjugated Fab antibody just after blocking with 5% normal serum.Samples with high levels of endogenous immunoglobulins may require up to 100 μg/mlof unconjugated Fab. To avoid a possible displacement of the Fab antibody by the labeledsecondary antibody, a light post-fixation with glutaraldehyde may be necessary, providedthat it does not affect antigenicity of the target proteins. Fab antibodies are not effectivefor blocking immunoglobulins in Western blotting or ELISA applications.

46 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.

Monovalent Fab Fragment Affinity-Purified Antibodiesfor Blocking and for Double Labeling Primary Antibodies from the Same Host Species


Donkey Anti-Goat IgG (H+L)***



Goat Anti-Human IgM, Fc5μ fragment specific

Donkey Anti-Mouse IgG (H+L)


Goat Anti-Mouse IgM, μ chain specific


Donkey Anti-Rabbit IgG (H+L)


Donkey Anti-Rat IgG (H+L)






0.5 ml705-067-003

0.5 ml305-067-003

0.5 ml109-067-003

0.5 ml109-067-043

0.5 ml715-067-003

0.5 ml115-067-003

0.5 ml115-067-020

0.5 ml315-067-003

0.5 ml711-067-003

0.5 ml111-067-003

0.5 ml712-067-003

0.5 ml112-067-003

0.5 ml313-067-003

0.75 mg705-607-003

0.5 mg305-607-003

0.75 mg 109-607-003

0.75 mg 109-607-043

0.75 mg 715-607-003

0.75 mg 115-607-003

0.75 mg 115-607-020

0.5 mg 315-607-003

0.75 mg 711-607-003

0.75 mg 111-607-003

0.75 mg 712-607-003

0.75 mg 112-607-003

0.5 mg 313-607-003

0.75 mg705-587-003

0.5 mg305-587-003

0.75 mg 109-587-003

0.75 mg 109-587-043

0.75 mg 715-587-003

0.75 mg 115-587-003

0.75 mg 115-587-020

0.5 mg 315-587-003

0.75 mg 711-587-003

0.75 mg111-587-003

0.75 mg 712-587-003

0.75 mg 112-587-003

0.5 mg 313-587-003

1.0 mg705-297-003

0.5 mg305-297-003

1.0 mg109-297-003

1.0 mg109-297-043

1.0 mg715-297-003

1.0 mg115-297-003

1.0 mg 115-297-020

0.5 mg315-297-003

1.0 mg711-297-003

1.0 mg111-297-003

1.0 mg712-297-003

1.0 mg112-297-003

0.5 mg 313-297-003

1.0 mg705-167-003

0.5 mg305-167-003

1.0 mg109-167-003

1.0 mg109-167-043

1.0 mg715-167-003

1.0 mg115-167-003

1.0 mg 115-167-020

0.5 mg315-167-003

1.0 mg711-167-003

1.0 mg111-167-003

1.0 mg712-167-003

1.0 mg112-167-003

0.5 mg 313-167-003

1.0 mg705-097-003

0.5 mg305-097-003

1.0 mg109-097-003

1.0 mg109-097-043

1.0 mg715-097-003

1.0 mg115-097-003

1.0 mg 115-097-020

0.5 mg315-097-003

1.0 mg711-097-003

1.0 mg111-097-003

1.0 mg712-097-003

1.0 mg112-097-003

0.5 mg 313-097-003


RhodamineRed-X RRX A=570, E=590


1.0 mg705-007-003

1.0 mg305-007-003

1.0 mg109-007-003

1.0 mg109-007-043

1.0 mg715-007-003

1.0 mg115-007-003

1.0 mg 115-007-020

1.0 mg315-007-003

1.0 mg711-007-003

1.0 mg111-007-003

1.0 mg712-007-003

1.0 mg112-007-003

1.0 mg313-007-003

0.75 mg705-547-003

0.5 mg305-547-003

0.75 mg 109-547-003

0.75 mg 109-547-043

0.75 mg 715-547-003

0.75 mg 115-547-003

0.75 mg 115-547-020

0.5 mg 315-547-003

0.75 mg 711-547-003

0.75 mg 111-547-003

0.75 mg 712-547-003

0.75 mg 112-547-003

0.5 mg 313-547-003

Unconjugated Alexa Fluor® 647 A=651, E=667




48


Example A: Use of conjugated Fab fragments for labeling and blocking.

Rabbit Anti-Antigen X Goat Anti-Rabbit IgG (H+L)

Rabbit Anti-Antigen Y

Fab fragment Goat Anti-Rabbit IgG (H+L)

Alexa Fluor® 488

Rhodamine Red-X

KEY

Fab Fragments for Blocking and Double Labeling of Primary Antibodies fromthe Same Host SpeciesThe following examples show some of the possible protocols used for double labelingtwo unconjugated primary antibodies from the same host species. The success of theseexperimental designs will require some empirical manipulations. In each case it is importantto label the less abundant primary antibody first. Trying different concentrations of reagentsin each step or switching the labeling sequence of the two antigens may influence theoutcome. Blocking with an appropriate normal serum between certain steps may alsohelp to reduce background. To avoid a possible displacement of the Fab antibody by thelabeled secondary antibody, a light post-fixation with glutaraldehyde may be necessary,provided that it does not affect antigenicity of the target proteins.

Important note: Monovalent Fab fragments have not been adsorbed to remove cross-reactivities to other species. If the cells/tissues under study have endogenousimmunoglobulins, using Example A or B may create background. In this case Example Cshould be used.

1 2 3

1. Incubate with the first primary antibody, in this example Rabbit Anti-Antigen X. Wash.

2. Incubate with excess Probe I-conjugated secondary antibody, in this example Alexa Fluor® 488-Fabfragment Goat Anti-Rabbit IgG (H+L). Wash.

3. Incubate with the second primary antibody, Rabbit Anti-Antigen Y, followed by Probe II-conjugatedsecondary antibody, in this example Rhodamine Red-X-Goat Anti-Rabbit IgG (H+L). Wash.

Caution: This protocol may be subject to the following problems: (1) Example A may require a high concentration of conjugated Fab to achieve effective blocking of the first primaryantibody. If this results in unacceptable background, try a lower concentration of the conjugated Fab, followedby further blocking with unconjugated Fab.

(2) If small aggregates of conjugated Fab are bound to the first primary antibody, they may act as divalent orpolyvalent antibodies and capture some of the second primary antibodies, which would result in overlappingdetection of antigens.




Example B: Use of unconjugated Fab fragments to cover the first primary antibody, presenting itas a different species. Rabbit Anti-Antigen X Mouse Anti-Rabbit IgG (H+L) (min X

Hu, Gt, Ms, Shp Sr Prot)



Alexa Fluor® 488

Rhodamine Red-X

KEY

Mouse Anti-Goat IgG (H+L)(min X Hu,Ms, Rb Sr Prot)

1 2 3 4 5


2. Incubate with an excess of unconjugated Fab antibody against the host species of the primary antibody, inthis example unconjugated Fab fragment Goat Anti-Rabbit IgG (H+L). This presents the rabbit IgG as goatFab. Wash.

3. Incubate with Probe I-conjugated tertiary antibody directed against the host species of the Fab antibody. Thetertiary antibody must not recognize the host species of either the primary antibodies or the secondsecondary antibody. This example used Alexa Fluor® 488-Mouse Anti-Goat IgG (H+L) (min X Ms, Hu, RbSr Prot). Wash.

4. Incubate with the second primary antibody, in this example Rabbit Anti-Antigen Y. Wash.

5. Incubate with Probe II conjugated to the second secondary antibody, that does not recognize the hostspecies of either the Fab antibody used in step 2 or the tertiary antibody used in step 3. In this example,Rhodamine Red-X-Mouse Anti-Rabbit IgG (H+L) (min X Hu, Gt, Ms, Shp Sr Prot) was used. Wash.

50


Example C: Use of unconjugated Fab fragments for blocking after the first secondary antibodystep. Rabbit Anti-Antigen X Alexa Fluor® 488



Rhodamine Red-X

Rabbit IgG from normal rabbit serum

KEY

Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms, Rat Sr Prot)


2. Incubate with Probe I-conjugated secondary antibody, in this example Alexa Fluor® 488-Goat Anti-Rabbit IgG(H+L) (min X Hu, Ms, Rat Sr Prot). Wash.

3. Incubate with normal serum from the same host species as the primary antibodies, in this example normalrabbit serum. The purpose of this step is to saturate open binding sites on the first secondary antibody withIgG so that they cannot capture the second primary antibody. Wash.

4. Incubate with an excess of unconjugated Fab antibody against the host species of the primary antibodies, inthis example Fab Goat Anti-Rabbit IgG (H+L).The host species of the Fab antibody should be the same asthe host species of the conjugated secondary antibody. This step covers the rabbit IgG so that the secondsecondary antibody will not bind to it. Wash.

5. Incubate with the second primary antibody, in this example Rabbit Anti-Antigen Y. Wash.

6. Incubate with Probe II conjugated to the same secondary antibody as used in step 2, in this exampleRhodamine Red-X-Goat Anti-Rabbit IgG (H+L)(min X Hu, Ms, Rat Sr Prot). Wash.

1 2 3 4 5 6




Example D: Use of unconjugated Fab fragments for detection of one unlabeled and one or morelabeled primary antibodies.

Examples D and E illustrate multiple labeling of primary antibodies from the same hostspecies when one or more of the primaries is directly labeled and does not require asecondary antibody. If it is necessary to incubate with the labeled primary antibody first(Example D), blocking with a monovalent Fab antibody is required to prevent the secondaryantibody used in a subsequent step from binding to the labeled primary antibody.Incubating with the unlabeled primary antibody first (Example E) will obviate the need forblocking with a monovalent Fab antibody.

1. Incubate with Probe I conjugated to the first primary antibody, in this example Alexa Fluor® 488-Rabbit Anti-Antigen X. Wash.

2. Incubate with an excess of unconjugated Fab Goat Anti-Rabbit IgG (H+L). Wash.

3. Incubate with the unconjugated primary antibody, in this example Rabbit Anti-Antigen Y. Wash.

4. Incubate with Probe II-conjugated secondary antibody, in this example Rhodamine Red-X-Goat Anti-RabbitIgG (H+L). Wash.

Rabbit Anti-Antigen X Goat Anti-Rabbit IgG (H+L)



Alexa Fluor® 488

Rhodamine Red-X

KEY

1 2 3 4

52


Rabbit Anti-Antigen X

Rabbit IgG from normal rabbit serum



Alexa Fluor® 488

Rhodamine Red-X

KEYExample E: Detection of one unlabeled and one or more labeled primary antibodies without the useof Fab fragments.


2. Incubate with Probe I-conjugated secondary antibody, in this example Alexa Fluor® 488-Goat Anti-Rabbit IgG(H+L). Wash.

3. Incubate with normal serum from the host species of the primary antibody, in this example normal rabbitserum. Wash.

4. Incubate with Probe II-conjugated primary antibody, in this example Rhodamine Red-X-Rabbit Anti-Antigen Y.Wash.

1 2 3 4


Secondary Antibodies for Flow Cytometry


For flow cytometry we offer three fluorescent proteins (R-PE, APC, and PerCP) conjugatedto many highly adsorbed secondary antibodies, streptavidin, and purified immunoglobulincontrols. The table of Secondary Antibodies for Flow Cytometry lists all of the antibodies,purified immunoglobulin controls, and streptavidin to which we conjugate these proteins.Also shown in this table are the same highly adsorbed antibodies and purifiedimmunoglobulins conjugated to Biotin-SP and fluorescent dyes appropriate for flowcytometry (Alexa Fluor® 488, FITC, and Alexa Fluor® 647). Note that many antibodies listedelsewhere in tables of Whole IgG and F(ab')2 Fragments also can be used for flow cytometry.

Phycoerythrin (R-PE) and allophycocyanin (APC) are among several kinds of light-harvestingphycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the formfound in red macrophytic algae (seaweed). APC is isolated from the blue-green alga Spirulina,and is chemically cross-linked for stability. After phycobiliproteins are conjugated to secondaryantibodies, there is little fluorescence quenching, which results in conjugates of high specificfluorescence compared with conventional fluorophore-antibody conjugates. R-PE, PerCP,

and APC can be excited by light over a wide range of the visible spectrum (Figure 6), arehighly water soluble, have relatively low isoelectric points, and lack potentially stickycarbohydrates.

PerCP is a fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. Weoffer the form found in Dinophyceae sp. with a molecular weight of about 35.5 kDa. It has abroad spectrum of excitation with a main peak at 482 nm, and a long Stokes shift to anemission peak at 677 nm (Figure 6).

PerCP, Alexa Fluor® 488 (or FITC), and R-PE are excited at 488 nm with an argon laser(Figure 6), and thus can be used for one-, two-, and three-color analyses with single-laserflow cytometers. APC and Alexa Fluor® 647 are excited at 633 nm (Figure 6) to give a fourthcolor with dual-laser flow cytometers.

It should be noted that the relatively high molecular weights of PerCP, R-PE, and APC maypreclude their use in procedures requiring good penetration into cells and tissues. They arepredominantly intended for surface labeling of cells for flow cytometry.

Exci

tatio

n

Wavelength (nm)700 750650600550500400 450300

0350

Figure 6. Excitation (left) and emission (right) spectra of Alexa Fluor®488/FITC (green), R-PE (red), PerCP (blue), Alexa Fluor® 647 (black),and APC (brown). Peak heights were normalized after the spectrawere obtained with an M-series spectrofluorometer system from PhotonTechnology International, Inc.

Fluo

resc

ence

Wavelength (nm)700 750650600550450

0500

Figure 6.


54 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.



F(ab')2 Fragment Donkey Anti-Chicken IgY† (IgG) (H+L)(min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)***(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

F(ab')2 Fragment Donkey Anti-Guinea Pig IgG (H+L)(min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Whole IgG Goat Anti-Armenian Hamster IgG (H+L) (min X Bov, Hu, Ms, Rb, Rat Sr Prot)**

F(ab')2 Fragment Donkey Anti-Human IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)

F(ab')2 Fragment Donkey Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot)

F(ab')2 Fragment Donkey Anti-Human IgM, Fc5μ fragment specific(min X Bov, Hrs Sr Prot)

F(ab')2 Fragment Goat Anti-Human IgG (H+L)(min X Bov, Hrs, Ms Sr Prot)

F(ab')2 Fragment Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Hrs, Ms Sr Prot)

Whole IgG Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot)

F(ab')2 Fragment Goat Anti-Human IgG, Fcγ fragment specific(min X Bov, Ms, Rb Sr Prot)

F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot)

F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L)(min X Bov Sr Prot)

Whole IgG Goat Anti-Human IgA, α chain specific

F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

0.5 mg703-006-155

0.5 mg 705-006-147

0.5 mg 706-006-148

1.0 mg 127-005-160

0.5 mg 709-006-149

1.0 mg 709-006-098

1.0 mg 709-006-073

1.0 mg 109-006-088

1.0 mg109-006-098

1.0 mg 109-005-098

1.0 mg 109-006-170

1.0 mg 109-006-097

1.0 mg 109-006-127

2.0 mg 109-005-011

0.5 mg 715-006-150

0.3 mg703-546-155

0.3 mg 705-546-147

0.3 mg 706-546-148

0.5 mg 127-545-160

0.3 mg 709-546-149

0.75 mg 709-546-098

0.75 mg 709-546-073

0.75 mg 109-546-088

0.75 mg 109-546-098

1.0 mg 109-545-098

0.75 mg 109-546-170

0.75 mg 109-546-097

0.75 mg 109-546-127

1.5 mg 109-545-011

0.3 mg 715-546-150


ML*

ML*

ML*

ML*

ML*




0.3 ml703-066-155

0.3 ml 705-066-147

0.3 ml 706-066-148

0.5 ml 127-065-160

0.3 ml 709-066-149

0.5 ml 709-066-098

0.5 ml 709-066-073

0.5 ml 109-066-088

0.5 ml 109-066-098

1.0 ml 109-065-098

0.5 ml 109-066-170

0.5 ml 109-066-097

0.5 ml 109-066-127

2.0 ml109-065-011

0.3 ml 715-066-150

0.5 ml703-126-155

0.5 ml 705-126-147

0.5 ml 706-126-148

0.5 ml 127-125-160

0.5 ml 709-126-149

0.5 ml 709-126-098

0.5 ml 709-126-073

0.5 ml 109-126-088

0.5 ml 109-126-098

0.5 ml 109-125-098

0.5 ml 109-126-170

0.5 ml109-126-097

0.5 ml 109-126-127

0.5 ml 109-125-011

0.5 ml 715-126-150

0.5 ml703-136-155

0.5 ml 705-136-147

0.5 ml 706-136-148

0.5 ml 127-135-160

0.5 ml 709-136-149

0.5 ml 709-136-098

0.5 ml 709-136-073

0.5 ml 109-136-088

0.5 ml 109-136-098

0.5 ml 109-135-098

0.5 ml 109-136-170

0.5 ml109-136-097

0.5 ml 109-136-127

0.5 ml 109-135-011

0.5 ml 715-136-150

0.3 mg703-606-155

0.3 mg 705-606-147

0.3 mg 706-606-148

0.5 mg 127-605-160

0.3 mg 709-606-149

0.75 mg 709-606-098

0.75 mg 709-606-073

0.75 mg 109-606-088

0.75 mg 109-606-098

1.0 mg 109-605-098

0.75 mg 109-606-170

0.75 mg 109-606-097

0.75 mg 109-606-127

1.5 mg 109-605-011

0.3 mg 715-606-150

1.0 ml703-116-155

1.0 ml 705-116-147

1.0 ml 706-116-148

1.0 ml 127-115-160

1.0 ml 709-116-149

1.0 ml 709-116-098

1.0 ml 709-116-073

1.0 ml 109-116-088

1.0 ml109-116-098

1.0 ml 109-115-098

1.0 ml 109-116-170

1.0 ml109-116-097

1.0 ml 109-116-127

1.0 ml109-115-011

1.0 ml 715-116-150

0.3 mg703-096-155

0.3 mg 705-096-147

0.3 mg 706-096-148

0.5 mg 127-095-160

0.3 mg 709-096-149

1.0 mg 709-096-098

1.0 mg 709-096-073

1.0 mg109-096-088

1.0 mg109-096-098

1.0 mg 109-095-098

1.0 mg 109-096-170

1.0 mg 109-096-097

1.0 mg 109-096-127

2.0 mg 109-095-011

0.3 mg 715-096-150


PerCPA=488, E=675

Allophycocyanin APCA=650, E=660



Phycoerythrin R-PEA=488, E=580




F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)**

F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Sw Sr Prot)

F(ab')2 Fragment Goat Anti-Mouse IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)

Whole IgG Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 1 specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2a specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2b specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2c specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 3 specific(min X Hu, Bov, Rb Sr Prot)

F(ab')2 Fragment Goat Anti-Mouse IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Goat Anti-Mouse IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Goat Anti-Mouse IgM, μ chain specific(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)

F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)(min X Hu, Ms, Rat Sr Prot)

F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

0.5 mg715-006-151

1.0 mg115-006-146

1.0 mg115-006-071

1.0 mg115-005-164

1.0 mg115-005-205

1.0 mg115-005-206

1.0 mg115-005-207

1.0 mg115-005-208

1.0 mg115-005-209

1.0 mg115-006-072

1.0 mg115-006-068

1.0 mg115-006-075

0.5 mg711-006-152

1.0 mg111-006-144

0.5 mg712-006-150

0.3 mg715-546-151

0.75 mg115-546-146

0.75 mg115-546-071

1.0 mg115-545-164

0.5 mg115-545-205

0.5 mg115-545-206

0.5 mg115-545-207

0.5 mg115-545-208

0.5 mg115-545-209

0.75 mg115-546-072

0.75 mg115-546-068

0.75 mg115-546-075

0.3 mg711-546-152

0.75 mg111-546-144

0.3 mg712-546-150


ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

57



0.3 ml 715-066-151

0.5 ml 115-066-146

0.5 ml 115-066-071

1.0 ml 115-065-164

0.5 ml 115-065-205

0.5 ml 115-065-206

0.5 ml 115-065-207

0.5 ml115-065-208

0.5 ml 115-065-209

0.5 ml115-066-072

0.5 ml 115-066-068

0.5 ml 115-066-075

0.3 ml711-066-152

0.5 ml 111-066-144

0.3 ml 712-066-150

0.5 ml 715-126-151

0.5 ml115-126-146

0.5 ml 115-126-071

0.5 ml 115-125-164

0.3 ml 115-125-205

0.3 ml 115-125-206

0.3 ml 115-125-207

0.3 ml115-125-208

0.3 ml 115-125-209

0.5 ml115-126-072

0.5 ml 115-126-068

0.5 ml 115-126-075

0.5 ml 711-126-152

0.5 ml 111-126-144

0.5 ml 712-126-150

0.5 ml 715-136-151

0.5 ml115-136-146

0.5 ml 115-136-071

0.5 ml 115-135-164

0.3 ml 115-135-205

0.3 ml 115-135-206

0.3 ml 115-135-207

0.3 ml115-135-208

0.3 ml 115-135-209

0.5 ml115-136-072

0.5 ml 115-136-068

0.5 ml 115-136-075

0.5 ml 711-136-152

0.5 ml 111-136-144

0.5 ml 712-136-150

0.3 mg 715-606-151

0.75 mg 115-606-146

0.75 mg 115-606-071

1.0 mg 115-605-164

0.5 mg 115-605-205

0.5 mg 115-605-206

0.5 mg 115-605-207

0.5 mg115-605-208

0.5 mg115-605-209

0.75 mg115-606-072

0.75 mg 115-606-068

0.75 mg 115-606-075

0.3 mg 711-606-152

0.75 mg 111-606-144

0.3 mg 712-606-150

1.0 ml 715-116-151

1.0 ml115-116-146

1.0 ml 115-116-071

1.0 ml 115-115-164

0.5 ml 115-115-205

0.5 ml 115-115-206

0.5 ml 115-115-207

0.5 ml115-115-208

0.5 ml 115-115-209

1.0 ml115-116-072

1.0 ml 115-116-068

1.0 ml 115-116-075

1.0 ml 711-116-152

1.0 ml 111-116-144

1.0 ml 712-116-150

0.3 mg 715-096-151

1.0 mg115-096-146

1.0 mg 115-096-071

1.0 mg 115-095-164

0.5 mg 115-095-205

0.5 mg 115-095-206

0.5 mg 115-095-207

0.5 mg115-095-208

0.5 mg 115-095-209

1.0 mg115-096-072

1.0 mg 115-096-068

1.0 mg 115-096-075

0.3 mg 711-096-152

1.0 mg 111-096-144

0.3 mg 712-096-150


PerCPA=488, E=675









F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)**

F(ab')2 Fragment Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Rb Sr Prot)

F(ab')2 Fragment Goat Anti-Rat IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Goat Anti-Rat IgG, F(ab')2 fragment specific(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Goat Anti-Rat IgM, μ chain specific(min X Hu, Bov, Hrs Sr Prot)

F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L)***(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Streptavidin

ChromPure Goat IgG, whole molecule

ChromPure Goat IgG, F(ab')2 fragment

ChromPure Donkey IgG, F(ab')2 fragment

0.3 mg712-546-153

0.75 mg112-546-143

0.75 mg112-546-071

0.75 mg112-546-072

0.75 mg112-546-075

0.3 mg713-546-147

1.0 mg 016-540-084

1.0 mg 005-540-003

1.0 mg 005-540-006

1.0 mg 017-540-006

Unconjugated

Streptavidin and IgG Controls Unconjugated



IgG Controls and Streptavidin Conjugates for Flow Cytometry

ML*

ML*

ML*

ML*

ML*

0.5 mg712-006-153

1.0 mg112-006-143

1.0 mg112-006-071

1.0 mg112-006-072

1.0 mg112-006-075

0.5 mg713-006-147

1.0 mg 016-000-084

10.0 mg005-000-003

2.0 mg 005-000-006

2.0 mg017-000-006

59




PerCPA=488, E=675




Fluorescein FITC/DTAFA=488, E=520



PerCPA=488, E=675




0.3 ml 712-066-153

0.5 ml 112-066-143

0.5 ml112-066-071

0.5 ml112-066-072

0.5 ml 112-066-075

0.3 ml 713-066-147

1.0 mg005-060-003

1.0 mg005-060-006

1.0 mg 017-060-006

0.5 ml 712-126-153

0.5 ml 112-126-143

0.5 ml 112-126-071

0.5 ml 112-126-072

0.5 ml 112-126-075

0.5 ml 713-126-147

0.5 ml 016-120-084

0.5 ml 005-120-003

0.5 ml 005-120-006

0.5 ml 017-120-006

0.5 ml 712-136-153

0.5 ml 112-136-143

0.5 ml112-136-071

0.5 ml 112-136-072

0.5 ml 112-136-075

0.5 ml 713-136-147

0.5 ml 016-130-084

0.5 ml 005-130-003

0.5 ml 005-130-006

0.5 ml 017-130-006

0.3 mg 712-606-153

0.75 mg 112-606-143

0.75 mg112-606-071

0.75 mg 112-606-072

0.75 mg 112-606-075

0.3 mg713-606-147

1.0 mg016-600-084

1.0 mg005-600-003

1.0 mg005-600-006

1.0 mg017-600-006

1.0 ml 712-116-153

1.0 ml 112-116-143

1.0 ml112-116-071

1.0 ml 112-116-072

1.0 ml 112-116-075

1.0 ml713-116-147

1.0 ml 016-110-084

1.0 ml 005-110-003

1.0 ml 005-110-006

1.0 ml 017-110-006

0.3 mg 712-096-153

1.0 mg112-096-143

1.0 mg112-096-071

1.0 mg 112-096-072

1.0 mg 112-096-075

0.3 mg 713-096-147

1.0 mg016-010-084 (DTAF)

1.0 mg 005-090-003 (FITC)

1.0 mg 005-090-006 (FITC)

1.0 mg 017-090-006 (FITC)



Anti-Mouse IgG Subclass Specific Antibodies


Whole IgG Goat Anti-Mouse IgG, Fcγ Subclass 1 specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ Subclass 2a specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ Subclass 2b specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ Subclass 2c specific(min X Hu, Bov, Rb Sr Prot)

Whole IgG Goat Anti-Mouse IgG, Fcγ Subclass 3 specific(min X Hu, Bov, Rb Sr Prot)

1.0 mg115-005-205

1.0 mg115-005-206

1.0 mg115-005-207

1.0 mg115-005-208

1.0 mg115-005-209

0.5 mg115-475-205

0.5 mg115-475-206

0.5 mg115-475-207

0.5 mg115-475-208

0.5 mg115-475-209

0.5 mg 115-545-205

0.5 mg115-545-206

0.5 mg115-545-207

0.5 mg115-545-208

0.5 mg115-545-209

Unconjugated DyLight 405A=400, E=421


These antibodies have been extensively adsorbed against other mouse IgG subclasses.They are intended for distinguishing between two or more different subclasses of mouseIgG in multiple-labeling experiments, or for mouse IgG subclass determination. They arenot necessary for general detection of mouse monoclonal antibodies in single-labelingexperiments or in multiple-labeling experiments involving one mouse monoclonal andprimary antibodies from other species.

They have been adsorbed against human, bovine, and rabbit serum proteins to minimizeinterfering cross-reactivities with tissue immunoglobulins, adherent bovine IgG on culturedcells, and rabbit primary antibodies.

ML*

ML*

ML*

ML*

ML*


Anti-Mouse IgG Subclass Specific Antibodies


0.5 ml115-055-205

0.5 ml115-055-206

0.5 ml115-055-207

0.5 ml115-055-208

0.5 ml115-055-209

0.5 ml115-065-205

0.5 ml115-065-206

0.5 ml115-065-207

0.5 ml115-065-208

0.5 ml115-065-209

0.5 ml115-035-205

0.5 ml115-035-206

0.5 ml115-035-207

0.5 ml115-035-208

0.5 ml115-035-209

0.5 mg 115-605-205

0.5 mg115-605-206

0.5 mg115-605-207

0.5 mg115-605-208

0.5 mg115-605-209

0.5 mg 115-585-205

0.5 mg115-585-206

0.5 mg115-585-207

0.5 mg115-585-208

0.5 mg 115-585-209

0.5 mg115-295-205

0.5 mg115-295-206

0.5 mg115-295-207

0.5 mg115-295-208

0.5 mg115-295-209

0.5 mg115-165-205

0.5 mg115-165-206

0.5 mg115-165-207

0.5 mg115-165-208

0.5 mg115-165-209

0.5 mg115-095-205

0.5 mg115-095-206

0.5 mg115-095-207

0.5 mg115-095-208

0.5 mg115-095-209

AlkalinePhosphatase







FluoresceinFITC

A=492, E=520

62 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.‡ This antibody reacts primarily with kappa light chains. It is not suitable for detection of primary antibodies with lambda light chains.

Anti-IgG, Light Chain Specific Antibodies for Western Blotting after IP

Anti-IgG, Light Chain Specific antibodies react with native primary antibodies used fordetecting specific protein bands on Western blots (Figure 7 A). If diluted properly anti-lightchain specific antibodies do not bind to the reduced and denatured IgG heavy chain band(50 kDa) on blots (Figure 7 B, D, E, and F). Therefore, by using anti-light chain specificantibodies, detection of antigens with molecular weights near 50 kDa is not obscured bylarge amounts of reduced and denatured IgG heavy chains from primary antibodies usedfor immunoprecipitation (IP). Although the antibodies react strongly with native IgG lightchains, some do not react as strongly with reduced and denatured light chains on blots(see for example 7A and D). Therefore, they are not recommended for sensitive andquantitative detection of reduced and denatured light chains.

The antibodies have been thoroughly adsorbed to minimize cross-reactivity withimmunoglobulins from many other species, which also may be present on blots.

If the protein of interest has a reduced and denatured molecular weight near 25 kDa,anti-IgG, Fc fragment specific antibodies may be used to detect native IgG primaryantibodies without binding to the 25 kDa band of reduced and denatured IgG light chainson Western blots.



AffiniPure Goat Anti-Mouse IgG, Light Chain‡ Specific(min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig)

IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific(min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)

AffiniPure Goat Anti-Rat IgG, Light Chain‡ Specific(min X Bov, Gt, Hrs, Hu, Ms, Rb, Shp Ig)


Figure 7. Detection of primary antibodies made in goat (against a 50 kDa protein) bymouse anti-goat IgG, Light Chain (LC) specific (A). Heavy (50 kDa) and light (25 kDa)chains of reduced and SDS-denatured mouse IgG (B, C), goat IgG (D), rat IgG (E), andrabbit IgG (F) were separated by SDS-PAGE and detected on Western blots usingHRP-goat anti-mouse IgG, LC specific (B), HRP-goat anti-mouse IgG (H+L) (C), HRP-mouse anti-goat IgG, LC specific (D), HRP-goat anti-rat IgG, LC specific (E), andHRP-mouse anti-rabbit IgG, LC specific (F). No heavy chain band was detected evenon lanes heavily overloaded with IgG when anti-IgG, LC specific antibodies were usedfor detection (B, D, E, and F). However, both heavy and light chain bands were detectedwith anti-IgG (H+L) (C). The three left-hand lanes had reduced and SDS-denaturedgoat IgG (B, C, and E) or mouse IgG (D and F), and the two right lanes in A had reducedand SDS-denatured mouse IgG, all of which served as background controls. The twomiddle lanes in A and the five right lanes in D demonstrate the relatively weak reactivitythis antibody has with reduced and SDS-denatured light chains.


Anti-IgG, Light Chain Specific Antibodies for Western Blotting after IP


0.5 ml 205-052-176

0.5 ml115-055-174

0.5 ml211-052-171

0.5 ml112-055-175

0.5 ml213-052-177

0.5 ml 205-032-176

0.5 ml115-035-174

0.5 ml211-032-171

0.5 ml112-035-175

0.5 ml213-032-177

0.5 ml 205-062-176

0.5 ml115-065-174

0.5 ml211-062-171

0.5 ml112-065-175

0.5 ml213-062-177

0.5 mg 205-602-176

0.5 mg115-605-174

0.5 mg211-602-171

0.5 mg112-605-175

0.5 mg213-602-177

0.5 mg 205-582-176

0.5 mg115-585-174

0.5 mg211-582-171

0.5 mg112-585-175

0.5 mg213-582-177

0.5 mg 205-162-176

0.5 mg115-165-174

0.5 mg211-162-171

0.5 mg112-165-175

0.5 mg213-162-177

AlkalinePhosphatase




1.0 mg205-002-176

1.0 mg115-005-174

1.0 mg211-002-171

1.0 mg112-005-175

1.0 mg213-002-177

0.5 mg205-542-176

0.5 mg115-545-174

0.5 mg211-542-171

0.5 mg112-545-175

0.5 mg213-542-177

UnconjugatedHorseradishPeroxidase



Figure 7.

64

Alexa Fluor® 680 and Alexa Fluor® 790 Conjugates for High Sensitivity Western Blots

Antibodies conjugated with far-red- and infrared-emitting dyes are more sensitive than dyesemitting visible light due to lower fluorescence quenching of the conjugates, higher extinctioncoefficients of the dyes, and lower background autofluorescence (Figure 8). The increasedbrightness allows for a wider range of immunofluorescence detection and imagingmodalities. Far-red and infrared dye conjugates can be used for higher sensitivity Westernblots, quantitative Western blots, in-gel Western blots, microWestern arrays, in-cell Westernarrays, on-cell Western arrays, tissue section imaging, and other techniques that require thebrightest dyes.

Jackson ImmunoResearch offers the largest selection of Alexa Fluor® 680 and Alexa Fluor® 790 dyes conjugated with signal-enhancing primary antibodies, secondaryantibodies, streptavidin, and purified immunoglobulin controls for use in quantitativeWestern blots and other techniques requiring high sensitivity. The secondary antibodiesare adsorbed for multiple labeling to minimize cross-reactions with others species and/orwith other immunoglobulin classes.

Figure 8. Double immunofluorescence staining on a Western blot using Alexa Fluor® 680 far-red fluorescent dye(red) and Alexa Fluor® 790 infrared fluorescent dye (green). Two micrograms of mouse IgG and two micrograms ofgoat IgG (background control) were reduced and denatured with ß-mercaptoethanol and SDS. The heavy and lightchains were separated by electrophoresis in SDS-PAGE, transferred to nitrocellulose, and double labeled with a 1: 100,000 dilution of Alexa Fluor® 790-goat anti-mouse IgG, Fcγ subclass 1 specific (min X Hu, Bov, Rb Sr Prot),115-655-205, to detect IgG heavy chains and a 1:100,000 dilution of Alexa Fluor® 680-goat anti-mouse IgG, lightchain specific (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig), 115-625-174 (red) to detect IgG light chains. Fluorescencewas imaged in a LI-COR Odyssey® imager. Note the very faint bands in the goat IgG lane attesting to the extremebrightness of the dyes even at a dilution of 1:100,000.

*** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Figure 8.

65



Streptavidin and Antibody Description

Streptavidin





Goat Anti-Armenian Hamster IgG (H+L)(min X Bov, Hu, Ms, Rb, Rat Sr Prot)**



Goat Anti-Human IgM, Fc5µ fragment specific(min X Bov Sr Prot)

Donkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)

Donkey Anti-Mouse IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)**




Goat Anti-Mouse IgM, μ chain specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Mouse IgG, Fcγ subclass 1 specific(min X Hu, Bov, Rb Sr Prot)

0.5 mg 016-620-084

0.5 mg703-625-155

0.5 mg705-625-147

0.3 mg205-622-176

0.5 mg706-625-148

0.3 mg127-625-160

0.5 mg709-625-149

0.5 mg109-625-098

0.5 mg109-625-129

0.5 mg715-625-150

0.3 mg715-625-151

0.5 mg115-625-146

0.3 mg115-625-166

0.5 mg115-625-071

0.5 mg115-625-075

0.3 mg115-625-205

0.5 mg 016-650-084

0.5 mg703-655-155

0.5 mg705-655-147

0.3 mg205-652-176

0.5 mg706-655-148

0.3 mg127-655-160

0.5 mg709-655-149

0.5 mg109-655-098

0.5 mg109-655-129

0.5 mg715-655-150

0.3 mg715-655-151

0.5 mg115-655-146

0.3 mg115-655-166

0.5 mg 115-655-071

0.5 mg115-655-075

0.3 mg115-655-205



ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*


66 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).‡ This antibody reacts primarily with kappa light chains. It is not suitable for detection of primary antibodies with lambda light chains.



Goat Anti-Mouse IgG, Fcγ subclass 2a specific(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ subclass 2b specific(min X Hu, Bov, Rb Sr Prot)


Goat Anti-Mouse IgG, Light Chain Specific‡

(min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig)



IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific(min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)






Goat Anti-Rat IgM, μ chain specific(min X Hu, Bov, Hrs Sr Prot)

Goat Anti-Rat IgG, Light Chain Specific‡

(min X Bov, Gt, Hrs, Hu, Ms, Rb, Shp Ig)



0.3 mg115-625-206

0.3 mg115-625-207

0.3 mg115-625-209

0.3 mg115-625-174

0.5 mg711-625-152

0.5 mg111-625-144

0.3 mg211-622-171

0.5 mg 712-625-150

0.3 mg712-625-153

0.5 mg112-625-143

0.3 mg112-625-167

0.5 mg112-625-071

0.5 mg112-625-075

0.3 mg112-625-175

0.5 mg713-625-147

0.3 mg213-622-177

0.3. mg115-655-206

0.3. mg115-655-207

0.3 mg115-655-209

0.3 mg115-655-174

0.5 mg 711-655-152

0.5 mg111-655-144

0.3 mg211-652-171

0.5 mg 712-655-150

0.3 mg712-655-153

0.5 mg112-655-143

0.3 mg112-655-167

0.5 mg 112-655-071

0.5 mg 112-655-075

0.3 mg112-655-175

0.5 mg713-655-147

0.3 mg213-652-177



ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*





IgG Fraction Monoclonal Mouse Anti-Fluorescein

IgG Fraction Monoclonal Mouse Anti-Digoxin

IgG Fraction Monoclonal Mouse Anti-Biotin

Goat Anti-Horseradish Peroxidase

ChromPure Donkey IgG, whole molecule


ChromPure Mouse IgG, whole molecule

0.3 mg200-622-037

0.3 mg200-622-156

0.3 mg200-622-211

0.5 mg123-625-021

0.5 mg017-620-003

0.5 mg005-620-003

0.5 mg015-620-003

0.3 mg200-652-037

0.3 mg200-652-156

0.3 mg200-652-211

0.5 mg 123-655-021

0.5 mg 017-650-003

0.5 mg005-650-003

0.5 mg015-650-003



CONTROLS

68

Cy2, Cy3 and Cy5 Conjugates for Permanent Mounting

Among currently available fluorescent dyes, the cyanine dyes are better able to withstand theharsh dehydration and embedding conditions required for mounting sections in non-polarplastic media, such as DPX and Permount. The cyanine dyes are brighter in the non-polarenvironment than in an aqueous medium, resulting in less acquisition time in the confocalmicroscope than that required for DyLight and Alexa Fluor® dyes, even though those dyesare brighter in aqueous mounting media.

The major advantages of plastic over aqueous mounting media are brightness, contrast, andlongevity of the fluorescence. Cy2, Cy3, and Cy5 are available conjugated to a selection of

secondary antibodies for multiple labeling, streptavidin, and purified IgG controls. For multiplelabeling, see the table on pages 69-70 for our listings of Cy2, Cy3, and Cy5 conjugates.Figure 9 illustrates the discrete spectra that provide effective separation for multiple labeling.For single labeling protocols that do not require highly cross-adsorbed antibodies, chooseCy3-conjugated antibodies from the lists of whole IgG affinity-purified secondary antibodieson pages 14-35. Note that Cy3 conjugates are bright and photostable in the aqueousenvironment as well as in non-polar media. This is in contrast to Cy2 and Cy5, which areoutperformed in aqueous media by Alexa Fluor® conjugates.


Figure 9. Excitation (left) and emission (right) spectra ofCy2 (green), Cy3 (red), and Cy5 (purple).

Peak heights were normalized after the spectra wereobtained with an M-series spectrofluorometer system fromPhoton Technology International, Inc.

Exci

tatio

n

Wavelength (nm)700650600550500450400300

0350

Fluo

resc

ence

Wavelength (nm)750700650600550500400

0450

Figure 9.




Streptavidin and Antibody Description

Streptavidin






D��ic(min X Hu, Bov, Rb Sr Prot)

Goat Anti-Mouse IgG, Fcγ subclass 2b specific(min X Hu, Bov, Rb Sr Prot)


Donkey Anti-Rabbit IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)Goat Anti-Rabbit IgG (H+L)(min X Hu, Ms, Rat Sr Prot)






1.0 mg 016-220-084

0.5 mg703-225-155

0.5 mg705-225-147

0.5 mg706-225-148

0.5 mg709-225-149

0.5 mg715-225-150

0.5 mg715-225-151

1.5 mg115-225-146

0.5 mg115-225-166

1.5 mg115-225-071

1.0 mg115-225-075

0.5 mg 115-225-205

0.5 mg115-225-206

0.5 mg115-225-207

0.5 mg115-225-209

0.5 mg711-225-152

1.0 mg 016-170-084

0.5 mg703-175-155

0.5 mg705-175-147

0.5 mg706-175-148

0.5 mg709-175-149

0.5 mg715-175-150

0.5 mg715-175-151

1.5 mg115-175-146

0.5 mg115-175-166

1.5 mg115-175-071

1.0 mg115-175-075

0.5 mg 115-175-205

0.5 mg115-175-206

0.5 mg115-175-207

0.5 mg115-175-209

0.5 mg711-175-152



1.0 mg 016-160-084

0.5 mg703-165-155

0.5 mg705-165-147

0.5 mg 706-165-148

0.5 mg709-165-149

0.5 mg715-165-150

0.5 mg715-165-151

1.5 mg115-165-146

0.5 mg115-165-166

1.5 mg115-165-071

1.0 mg115-165-075

0.5 mg 115-165-205

0.5 mg115-165-206

0.5 mg115-165-207

0.5 mg115-165-209

0.5 mg711-165-152


ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*




Goat Anti-Rat IgM, μ chain specific(min X Hu, Bov, Hrs Sr Prot)


CONTROLS

ChromPure Donkey IgG, whole molecule


1.5 mg111-225-144

0.5 mg712-225-150

0.5 mg712-225-153

1.5 mg112-225-143

0.5 mg112-225-167

1.5 mg112-225-071

1.0 mg112-225-075

0.5 mg713-225-147

1.0 mg 017-220-003

1.0 mg005-220-003

1.5 mg111-175-144

0.5 mg712-175-150

0.5 mg712-175-153

1.5 mg112-175-143

0.5 mg112-175-167

1.5 mg112-175-071

1.0 mg112-175-075

0.5 mg713-175-147

1.0 mg 017-170-003

1.0 mg005-170-003



1.5 mg111-165-144

0.5 mg712-165-150

0.5 mg712-165-153

1.5 mg112-165-143

0.5 mg112-165-167

1.5 mg112-165-071

1.0 mg112-165-075

0.5 mg713-165-147

1.0 mg 017-160-003

1.0 mg005-160-003


ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-whiteavidin in its ability to bind biotin, and has been used as a replacement for egg-white avidinbecause of its more favorable chemical properties. Unlike egg-white avidin which has anet positive charge at neutral pH and contains about 7% carbohydrate, streptavidin hasalmost no net charge at neutral pH, does not contain carbohydrate, and exhibits lowernon-specific binding.

Conjugates of streptavidin are recommended for use with Biotin-SP-conjugatedantibodies (pages 14-45, 54-63, and 76-77) and Biotin-SP-conjugated ChromPureproteins (pages 80-89). Our research indicates that this system of covalently conjugatedreagents is more stable, gives less background, and is more sensitive than the avidin-biotin-enzyme complex (ABC) method. This has been independently verified by Shi et al.(J. Histochem. Cytochem. 1988. 36, 317) and Milde et al. (J. Histochem. Cytochem.1989. 37, 1609). The increased sensitivity may be due to enhanced tissue penetrationand less steric hindrance, since nominal molecular weights for all components of theconjugated streptavidin system are less than 200 kDa, considerably lower than those ofABC.

The following comprehensive list of fluorophores and enzymes conjugated to streptavidinprovides amplification options for use in enzyme immunoassays, immunohistochemistry,flow cytometry, in situ hybridization, and immunoblotting procedures. Most streptavidinproducts are freeze-dried in buffer containing stabilizers and a preservative. Exceptionsare peroxidase-conjugated streptavidin, (freeze-dried without preservative) and alkalinephosphatase-conjugated streptavidin (a sterile-filtered liquid containing stabilizers and apreservative).

71

Streptavidin Code Number Size

016-000-084

016-000-113

016-000-114

016-470-084

016-150-084

016-220-084

016-540-084

016-010-084

016-160-084

016-020-084

016-110-084

016-290-084

016-580-084

016-600-084

016-130-084

016-170-084

016-120-084

016-620-084

016-650-084

016-030-084

016-050-084

Unconjugated

Unconjugated

Unconjugated

DyLight 405 (A=400, E=421)

Aminomethylcoumarin, AMCA (A=350, E=450)

Cy2, Cyanine (A=492, E=510)

Alexa Fluor® 488 (A=493, E=519)

Fluorescein, DTAF‡‡ (A=492, E=520)

Cy3, Indocarbocyanine (A=550, E=570)

Tetramethyl Rhodamine, TRITC (A=550, E=570)

R-Phycoerythrin, R-PE (A=488, E=580)

Rhodamine Red-X, RRX (A=570, E=590)

Alexa Fluor® 594 (A=591, E=614)

Alexa Fluor® 647 (A=651, E=667)

Allophycocyanine, APC (A=650, E=660)

Cy5, Indodicarbocyanine (A=650, E=670)

PerCP (A=488, E=675)

Alexa Fluor® 680 (A=684, E=702)

Alexa Fluor® 790 (A=792, E=803)

Horseradish Peroxidase

Alkaline Phosphatase

1 mg

5 mg

10 mg

1 mg

1 mg

1 mg

1 mg

1 mg

1 mg

1 mg

1 ml

1 mg

1 mg

1 mg

0.5 ml

1 mg

0.5 ml

0.5 mg

0.5 mg

1 mg

1 mg‡‡ DTAF and FITC contain the same fluorescein molecule, and have identical peaks of excitation

and emission. However, DTAF is brighter than FITC when conjugated to streptavidin.

Streptavidin



72

Colloidal gold reagents are available either for transmission and scanning electronmicroscopy (EM Grade) or for light microscopy and immunoblotting (LM Grade). The EMGrade is distinguished from other commercial preparations by careful separation ofmonomeric particles from small aggregates using ultracentrifugation in density gradients.The resulting monomeric colloidal gold-protein complexes are suspended in sterile-filteredbuffer containing stabilizers and a preservative. All particle sizes in the EM Grade categorymay also be used for light microscopy and immunoblotting by those who prefer to useparticles larger than 4 nm.

Since signal intensity is relatively independent of particle size when silver enhancement isused, we offer 4 nm particles (LM Grade) because this size may penetrate tissues betterthan larger particles. The 4 nm size may be used for electron microscopy in studies thatrequire smaller particles since they are relatively uniform in size (coefficient of variationless than or equal to 15%), though small aggregates are not removed from this grade.Therefore, our 4 nm particles should only be used for electron microscopy with theunderstanding that the presence of small aggregates may give misleading results.

A detailed protocol for silver enhancement is provided with all orders for LM Gradeproducts. All reagents involved in the light-insensitive silver enhancement reaction can beprepared easily in the laboratory. However, those who wish to use commercially availablesilver enhancement kits can continue to do so. All LM Grade colloidal gold-proteincomplexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution,they may be frozen in aliquots for extended storage.

ImmunoGold Reagents


4 nm LM Grade Colloidal Gold - Antibody Complexes

0.5 ml301-185-003

0.5 ml703-185-155

0.5 ml304-185-003

0.5 ml705-185-147

0.5 ml706-185-148

0.5 ml308-185-003

1.0 ml109-185-088

1.0 ml 109-185-098

1.0 ml109-185-043

1.0 ml109-185-011

1.0 ml115-185-146

Rabbit Anti-Bovine IgG (H+L)***

Donkey Anti-Chicken IgY† (IgG)(H+L)(min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Rabbit Anti-Dog IgG (H+L)



Rabbit Anti-Horse IgG (H+L)***



Goat Anti-Human IgM, Fc5µ fragment specific



Antibody Description Size/CodeNumber

0.5 ml115-185-166

1.0 ml115-185-071

1.0 ml115-185-068

1.0 ml 115-185-075

0.5 ml711-185-152

1.0 ml111-185-144

1.0 ml112-185-143

0.5 ml112-185-167

1.0 ml112-185-075

0.5 ml713-185-147

1.0 ml123-185-021



Goat Anti-Mouse IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)






Goat Anti-Rat IgM, µ chain specific(min X Hu, Bov, Hrs Sr Prot)



Antibody Description Size/CodeNumber



ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

74 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.** Caution: See page 6 (min X ... Sr Prot) before selecting an antibody adsorbed against closely related species.* ML = Multiple Labeling (see Multiple Labeling on pages 6-7 for an explanation).† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

EM Grade Colloidal Gold - Antibody Complexes


Donkey Anti-Chicken IgY†(IgG)(H+L)(min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

Donkey Anti-Goat IgG (H+L)***(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)Donkey Anti-Guinea Pig IgG (H+L)(min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)



Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Sw Sr Prot)Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, Rat Sr Prot)**

Goat Anti-Mouse IgG, Fcγ fragment specific(min X Hu, Bov, Hrs Sr Prot)Goat Anti-Mouse IgG + IgM (H+L)(min X Hu, Bov, Hrs Sr Prot)


Donkey Anti-Rabbit IgG (H+L)(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)Goat Anti-Rabbit IgG (H+L)(min X Hu, Ms, Rat Sr Prot)

Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Rb Sr Prot)Goat Anti-Rat IgG (H+L)(min X Hu, Bov, Hrs, Ms, Rb Sr Prot)**Goat Anti-Rat IgM, µ chain specific(min X Hu, Bov, Hrs Sr Prot)



18 nm

0.3 ml703-215-155

0.3 ml705-215-147

0.3 ml706-215-148

0.5 ml109-215-088

0.3 ml715-215-150

0.5 ml115-215-146

0.3 ml115-215-166

0.5 ml 115-215-071

0.5 ml115-215-068

0.5 ml115-215-075

0.3 ml711-215-152

0.5 ml111-215-144

0.5 ml112-215-143

0.3 ml112-215-167

0.3 ml713-215-147

0.5 ml123-215-021

12 nm

0.3 ml703-205-155

0.3 ml705-205-147

0.3 ml706-205-148

0.5 ml109-205-088

0.3 ml715-205-150

0.5 ml115-205-146

0.3 ml115-205-166

0.5 ml 115-205-071

0.5 ml115-205-068

0.5 ml115-205-075

0.3 ml711-205-152

0.5 ml111-205-144

0.5 ml112-205-143

0.3 ml112-205-167

0.5 ml112-205-075

0.3 ml713-205-147

0.5 ml123-205-021

6 nm

0.3 ml703-195-155

0.3 ml705-195-147

0.3 ml706-195-148

0.5 ml109-195-088

0.3 ml715-195-150

0.5 ml115-195-146

0.3 ml115-195-166

0.5 ml 115-195-071

0.5 ml115-195-068

0.5 ml115-195-075

0.3 ml711-195-152

0.5 ml111-195-144

0.5 ml112-195-143

0.3 ml112-195-167

0.3 ml713-195-147

0.5 ml123-195-021

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*

ML*



Solid-Phase Immunoadsorbent Gels

PAP soluble immune complexes are prepared by the method of Sternberger et al. (J. Histochem. Cytochem. 1970. 18, 315). Theoretically, they consist predominantly of twoanti-horseradish peroxidase antibodies in soluble complex with three molecules of horseradishperoxidase.

PAP soluble complexes are normally used at 25-50 µg/ml. Whole antisera against IgG (H+L)are recommended for bridging PAP to primary antibodies. Antisera against IgG (H+L) oragainst the F(ab')2 fragments of IgG will also bridge PAP to IgM primary antibodies (such asIgM monoclonal antibodies) by virtue of common light-chain recognition. Normal serum (5%v/v) from the same host species as the bridging antibody is suggested as a blocking solutionto minimize non-specific binding. All PAP soluble complexes are freeze-dried at 20 mg/ml.

PAP soluble complexes, as well as other immunoperoxidase reagents, are not recommendedfor tissues or cells in which endogenous peroxidase activity is difficult to suppress. In suchcases, other immunoenzyme reagents may be used. Alternatively, anti-horseradish peroxidaseconjugated with other enzymes or fluorophores (page 76) may be used to enhance signal andreduce background, since the final signal does not depend on the enzyme activity ofperoxidase, but on the antigenicity of horseradish peroxidase.

Highly purified IgG (pages 80-89) from the serum of non-immunized animals is coupled tocyanogen bromide-activated 4% agarose gels for use in preparing affinity-purified antibodiesor removing cross-reactive antibodies. Proteins are coupled at a concentration of 1 mg proteinper ml of settled gel, and are packaged in phosphate buffered saline with sodium azide.

ChromPure IgG-Agarose Code Number Size

005-000-052

015-000-052

011-000-052

Goat IgG, whole molecule

Mouse IgG, whole molecule

Rabbit IgG, whole molecule

5 ml

5 ml

5 ml

PAP Soluble Immune Complex PAPConcentration Size

20 mg/ml

20 mg/ml

20 mg/ml

Code Number

123-005-024

223-005-024

323-005-024

Goat PAP

Mouse PAP

Rabbit PAP

0.25 ml

0.25 ml

0.25 ml

Peroxidase-Anti-Peroxidase (PAP) SolubleImmune Complexes

76

Miscellaneous Antibodies

Antibody Description Coumarin AMCAA=350, E=450Unconjugated DyLight 405

A=400, E=421Indocarbocyanine

Cy3A=550, E=570



IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, and Anti-Digoxin may beused either as direct conjugates, or for more sensitivity, they can be used unconjugatedfollowed by a conjugated anti-mouse IgG (H+L) for signal enhancement (see examplesbelow using Mouse Anti-Biotin).

Example 1.DNA, RNA or Tissue Antigen

Biotin-conjugated Antibody or Biotin-conjugated Nucleic Acid Probe

Fluorophore- or Enzyme-conjugated Monoclonal Mouse Anti-Biotin

Example 2.DNA, RNA or Tissue Antigen

Biotin-conjugated Nucleic Acid Probe or Biotin-conjugated Antibody

Unconjugated Monoclonal Mouse Anti-Biotin

Fluorophore-, Colloidal Gold-, or Enzyme-conjugated Anti-Mouse IgG (H+L)

ANTI-DIGOXIN

ANTI-BIOTIN

ANTI-FLUORESCEIN

ANTI-HORSERADISH PEROXIDASE

IgG Fraction Monoclonal Mouse Anti-Digoxin

IgG Fraction Monoclonal Mouse Anti-Biotin

IgG Fraction Monoclonal Mouse Anti-Fluorescein


Rabbit Anti-Horseradish Peroxidase

0.5 mg200-542-156

0.5 mg 200-542-211

0.5 mg 200-542-037

1.5 mg123-545-021

1.0 mg323-545-021

0.5 mg200-092-156

0.5 mg200-092-211

2.0 mg123-095-021

1.5 mg323-095-021

0.5 mg200-162-156

0.5 mg 200-162-211

0.5 mg 200-162-037

2 0 mg123-165-021

1.5 mg323-165-021

0.5 mg200-472-156

0.5 mg 200-472-211

0.5 mg 200-472-037

0.5 mg200-152-156

0.5 mg200-152-211

0.5 mg200-152-037

2.0 mg123-155-021

1.5 mg323-155-021

1.0 mg200-002-156

1.0 mg200-002-211

1.0 mg200-002-037

2.0 mg123-005-021

2.0 mg323-005-021

77


Miscellaneous Antibodies

AlkalinePhosphatase








Affinity-purified anti horseradish peroxidase (HRP) may be used to detect HRP or toenhance signal by binding to HRP-conjugated molecules (such as HRP-conjugatedantibodies or HRP-conjugated streptavidin) or HRP-containing complexes (such as PAPor HRP-ABC). Anti-HRP also may be used to convert an HRP conjugate into a differentsignal. For example, our anti-HRP complexed with colloidal gold particles has been usedwith silver enhancement in place of diaminobenzidine (DAB) to create less diffused imagesfollowing labeling of tissue sections with HRP reagents (Gee et al., J. Histochem. Cytochem.1991. 39, 863; Roth et al., Methods in Lab. Invest. 1992. 67, 263; Roth et al.,Histochemistry. 1992. 98, 229). A further advantage of the technique was a reduction inbackground staining from endogenous peroxidase-like enzymes in animal tissue. Thebackground, (typically detected with DAB), was not detected with anti-HRP, since HRPis a plant enzyme.

The procedure also may be used with fluorophore- or alkaline phosphatase-conjugatedanti-HRP to amplify and/or convert signals as shown in the examples below.See pages 73-74 for colloidal gold-anti-HRP complexes.

Tissue Antigen

Primary Antibody DNA or RNA

Biotinylated Secondary Antibody Biotinylated Nucleic Acid Probe

HRP-Streptavidin

Colloidal Gold-, Fluorophore-, or Alkaline Phosphatase-conjugated Anti-HRP

0.5 mg200-292-156

0.5 mg200-292-211

0.5 mg200-292-037

2.0 mg123-295-021

1.5 mg323-295-021

0.5 mg200-582-156

0.5 mg200-582-211

0.5 mg200-582-037

1.5 mg123-585-021

1.0 mg323-585-021

0.5 mg200-602-156

0.5 mg200-602-211

0.5 mg200-602-037

1.5 mg123-605-021

1.0 mg323-605-021

0.3 mg200-622-156

0.3 mg 200-622-211

0.3 mg 200-622-037

0.5 mg123-625-021

0.3 mg200-652-156

0.3 mg 200-652-211

0.3 mg200-652-037

0.5 mg123-655-021

0.5 ml200-062-156

0.5 ml200-062-037

2.0 ml123-065-021

1.5 ml323-065-021

0.5 ml200-032-156

0.5 ml200-032-211

0.5 ml200-032-037

0.5 ml200-052-156

0.5 ml200-052-211

0.5 ml200-052-037

1.0 ml123-055-021

1.0 ml323-055-021


Normal Serums and Gamma Globulins

78

Product Code Number Size Product

Normal Horse SerumNormal Horse SerumHorse Gamma Globulin

Normal Human SerumNormal Human SerumHuman Gamma Globulin

Normal Mouse SerumNormal Mouse SerumMouse Gamma Globulin

Normal Rabbit SerumNormal Rabbit SerumRabbit Gamma Globulin

Normal Rat SerumNormal Rat SerumRat Gamma Globulin

Normal Sheep SerumNormal Sheep SerumSheep Gamma Globulin

Normal Swine SerumNormal Swine SerumSwine Gamma Globulin

Normal Bovine SerumNormal Bovine SerumBovine Gamma Globulin

Normal Cat SerumNormal Cat SerumCat Gamma Globulin

Normal Chicken SerumNormal Chicken SerumChicken Gamma Globulin

Normal Dog SerumNormal Dog SerumDog Gamma Globulin

Normal Donkey SerumNormal Donkey SerumDonkey Gamma Globulin

Normal Goat SerumNormal Goat SerumGoat Gamma Globulin

Normal Guinea Pig SerumNormal Guinea Pig SerumGuinea Pig Gamma Globulin

Normal Syrian Hamster SerumNormal Syrian Hamster SerumSyrian Hamster Gamma Globulin

001-000-001001-000-121001-000-002

002-000-001002-000-120002-000-002

003-000-001003-000-120003-000-002

004-000-001004-000-120004-000-002

017-000-001017-000-121017-000-002

005-000-001005-000-121005-000-002

006-000-001006-000-120006-000-002

007-000-001007-000-120007-000-002

2 ml10 ml10 mg

2 ml5 ml

10 mg

2 ml5 ml

10 mg

2 ml5 ml

10 mg

2 ml10 ml10 mg

2 ml10 ml10 mg

2 ml5 ml

10 mg

2 ml5 ml

10 mg

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate bufferedsaline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce backgroundfrom non-specific, conserved sequence and/or Fc-receptor binding. Best results areobtained with diluted normal serum from the same host as the labeled antibody, as aseparate incubation step before addition of the primary antibody.

Gamma globulins are further purified from non-immunized animal serums by saltfractionation, ion-exchange chromatography and gel filtration. Gamma globulins are aninexpensive source of IgG with only trace amounts of other immunoglobulins and/or non-immunoglobulin serum proteins. Gamma globulins are supplied as sterile liquids inphosphate buffer without stabilizers or preservatives.

Code Number Size

008-000-001008-000-121008-000-002

009-000-001009-000-121009-000-002

015-000-001015-000-120015-000-002

011-000-001011-000-120011-000-002

012-000-001012-000-120012-000-002

013-000-001013-000-121013-000-002

014-000-001014-000-121014-000-002

2 ml10 ml10 mg

2 ml10 ml10 mg

2 ml5 ml

10 mg

2 ml5 ml

10 mg

2 ml5 ml

10 mg

2 ml10 ml10 mg

2 ml10 ml10 mg


Bovine serum albumin (BSA) is used extensively as a carrier protein to dilute antibodiesand as a general protein blocking agent in immunoassays and immunodetection protocols.Unfortunately, many commercial preparations of BSA, even some of the highest puritygrades, contain IgG that may become an antigen for cross-reacting secondary antibodies.This is particularly common when using anti-bovine IgG, anti-goat IgG (with the exceptionof bovine anti-goat IgG), rabbit anti-horse IgG, or anti-sheep IgG, but may occur withother antibodies that cross-react with bovine IgG. The result of these interactions maybe loss of desired antibody activity, loss of antibody stability, and/or increasedbackground. Background may derive from sticky soluble immune complexes or fromcontaminating bovine IgG sticking non-specifically and attracting cross-reacting labeledsecondary antibodies. Even small amounts of contaminating IgG may create theseproblems, due to the use of high concentrations of BSA in many protocols. Our IgG-freeBSA, which is also protease-free, alleviates many of these problems. IgG-free BSA issupplied as a pure protein, freeze-dried from deionized water. Please inquire aboutavailability of larger sizes.

Description Code Number Size

001-000-161

001-000-162

001-000-173

Bovine Serum Albumin (IgG-Free, Protease-Free)



10 g

50 g

250 g



80 † IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Purified Proteins from Normal Serums

ChromPure Proteins

IgG, whole molecule

IgG, Fc fragment

IgG, Fab fragment

BOVINE10 mg

001-000-003

2 mg001-000-008

2 mg001-000-007

1 mg001-470-003


CATIgG, whole molecule 10 mg

002-000-003

IgY† (IgG), whole molecule

IgY† (IgG), Fc fragment

IgY† (IgG), Fab fragment

5 mg003-000-003

1 mg003-000-008

2 mg003-000-007

CHICKEN

ChromPure is our trade name for highly purified proteins from the serum of non-immunizedanimals. The purified immunoglobulins in this section therefore do not represent antibodiesdirected against known antigens. ChromPure proteins are prepared by a variety ofmethods, including ion-exchange, gel-filtration, hydrophobic, dye-ligand, metal-affinity,Protein A, and immunoaffinity chromatographies. F(ab')2 (pepsin) and Fab and Fc (papain)fragments (see page 4, Figure 1) are purified from enzyme digests of highly purified wholemolecules. No contaminating whole molecules or undesired fragments are observed at aprotein concentration of 20 mg/ml when tested by immunoelectrophoresis against anti-whole serums, anti-immmunoglobulins (class-specific), or anti-fragment specific antisera.

However, these proteins are not guaranteed to produce monospecific antibodies whenused for immunization, nor should they be used as molecular weight markers. They shouldbe used primarily as experimental controls. Unconjugated ChromPure proteins aresupplied as sterile-filtered liquids without stabilizers or preservative. ConjugatedChromPure proteins are freeze-dried with stabilizers and a preservative, with the exceptionof peroxidase conjugates, which do not contain a preservative.

For a complete list of ChromPure protein conjugates, see www.jacksonimmuno.com.









1 mg001-030-003

1 mg001-060-003

1 mg001-600-003

1 mg001-580-003

1 mg001-160-003

1 mg001-090-003

1 mg001-540-003

1 mg003-030-003

1 mg003-060-003

1 mg003-600-003

1 mg003-580-003

1 mg003-160-003

1 mg003-090-003

1 mg003-540-003



82


ChromPure Proteins

IgG, whole molecule

IgG, Fc fragment

IgG, F(ab')2 fragment

IgG, Fab fragment

GOAT10 mg

005-000-003

1 mg005-000-008

2 mg005-000-006

2 mg005-000-007

1 mg005-470-003


GUINEA PIG

HAMSTER (Syrian)

IgG, whole molecule 10 mg006-000-003

IgG, whole molecule 5 mg007-000-003

HORSEIgG, whole molecule 10 mg

008-000-003

DOGIgG, whole molecule 10 mg

004-000-003

DONKEYIgG, whole molecule


10 mg017-000-003

2 mg017-000-006

1 mg017-470-003










1 mg005-030-003

1 mg005-030-006

1 mg005-060-003

1 mg005-060-006

1 mg005-600-003

1 mg005-600-006

1 mg005-580-003

1 mg005-160-003

1 mg005-160-006

1 mg005-090-003

1 mg005-090-006

1 mg005-540-003

1 mg005-540-006

1 mg007-030-003

1 mg007-060-003

1 mg007-160-003

1 mg007-090-003

1 mg017-030-003

1 mg017-030-006

1 mg017-060-003

1 mg017-060-006

1 mg017-600-003

1 mg017-600-006

1 mg017-580-003

1 mg017-160-003

1 mg017-160-006

1 mg017-090-003

1 mg017-090-006

1 mg017-540-003

1 mg017-540-006


84


ChromPure Proteins Unconjugated DyLight 405A=400, E=421

MOUSEIgG, whole molecule

IgG, Fc fragment


5 mg015-000-003

1 mg015-000-008

2 mg015-000-006

1 mg015-470-003

HORSEIgG, F(ab')2 fragment

IgG, Fab fragment

2 mg008-000-006

2 mg008-000-007

HUMANIgG, whole molecule

IgG, Fc fragment


IgG, Fab fragment

IgM (myeloma), whole molecule

Serum IgA

Albumin

Transferrin

10 mg009-000-003

1 mg009-000-008

2 mg009-000-006

2 mg009-000-007

2 mg009-000-012

2 mg009-000-011

5 mg009-000-051

5 mg009-000-050

1 mg009-470-003










1 mg015-030-003

1 mg015-030-008

1 mg015-030-006

1 mg015-060-003

1 mg015-060-008

1 mg015-060-006

1 mg015-600-003

1 mg015-600-006

1 mg015-580-003

1 mg015-160-003

1 mg015-160-008

1 mg015-160-006

1 mg015-090-003

1 mg015-090-008

1 mg015-090-006

1 mg015-540-003

1 mg015-540-006

1 mg009-030-003

1 mg009-030-008

1 mg009-030-006

1 mg009-030-007

1 mg009-030-012

1 mg009-030-011

1 mg009-030-051

1 mg009-030-050

1 mg009-060-003

1 mg009-060-008

1 mg009-060-006

1 mg009-060-007

1 mg009-060-012

1 mg009-060-011

1 mg009-060-051

1 mg009-060-050

1 mg009-600-003

1 mg009-600-008

1 mg009-600-006

1 mg009-600-007

1 mg009-600-012

1 mg009-600-051

1 mg009-600-050

1 mg009-580-003

1 mg009-160-003

1 mg009-160-008

1 mg009-160-006

1 mg009-160-007

1 mg009-160-012

1 mg009-160-011

1 mg009-160-051

1 mg009-160-050

1 mg009-090-003

1 mg009-090-008

1 mg009-090-006

1 mg009-090-007

1 mg009-090-012

1 mg009-090-011

1 mg009-090-051

1 mg009-090-050

1 mg009-540-003

1 mg009-540-008

1 mg009-540-006

1 mg009-540-007

1 mg009-540-012

1 mg009-540-051

1 mg009-540-050


86


ChromPure Proteins

MOUSE


RABBITIgG, whole molecule

IgG, Fc fragment


IgG, Fab fragment

10 mg011-000-003

1 mg011-000-008

2 mg011-000-006

2 mg011-000-007

RATIgG, whole molecule

IgG, Fc fragment


IgG, Fab fragment

Transferrin

10 mg012-000-003

1 mg012-000-008

2 mg012-000-006

2 mg012-000-007

5 mg012-000-050

1 mg012-470-003

IgG, Fab fragment

Transferrin

2 mg015-000-007

5 mg015-000-050

1 mg011-470-003









1 mg012-030-003

1 mg012-030-050

1 mg012-060-003

1 mg012-060-050

1 mg012-600-003

1 mg012-600-050

1 mg012-580-003

1 mg012-160-003

1 mg012-160-050

1 mg012-090-003

1 mg012-090-050

1 mg012-540-003

1 mg012-540-050

1 mg015-030-007

1 mg015-030-050

1 mg015-060-007

1 mg015-060-050

1 mg015-600-050

1 mg015-160-007

1 mg015-160-050

1 mg015-090-007

1 mg015-090-050

1 mg015-540-050

1 mg011-030-003

1 mg011-060-003

1 mg011-600-003

1 mg011-580-003

1 mg011-160-003

1 mg011-090-003

1 mg011-540-003



88


ChromPure Proteins Unconjugated

SHEEPIgG, whole molecule

IgG, Fc fragment

10 mg013-000-003

1 mg013-000-008

1.0 mg013-470-003

SWINEIgG, whole molecule 10 mg

014-000-003

DyLight 405A=400, E=421

www.jacksonimmuno.com Telephone: 800-367-5296 Fax: 610-869-0171 89









1 mg013-030-003

1 mg013-060-003

1 mg013-600-003

1 mg013-580-003

1 mg013-160-003

1 mg013-090-003

1 mg013-540-003


90 *** Warning: BSA and dry milk may contain IgG which will be recognized by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.† IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Antisera to Immunoglobulins, Whole Serums and Enzymes

Antiserum Code NumberHost Antiserum Code NumberHost

715-001-003

115-001-003115-001-008115-001-020115-001-001

315-001-003315-001-008

123-001-021

711-001-003

111-001-003111-001-008111-001-001

112-001-003112-001-008112-001-001

313-001-003

Anti-Mouse IgG (H+L)

Anti-Mouse IgG (H+L)Anti-Mouse IgG, Fc fragment specificAnti-Mouse IgM, µ chain specificAnti-Mouse Whole Serum

Anti-Mouse IgG (H+L)Anti-Mouse IgG, Fc fragment specific

Anti-Horseradish Peroxidase

Anti-Rabbit IgG (H+L)

Anti-Rabbit IgG (H+L)Anti-Rabbit IgG, Fc fragment specificAnti-Rabbit Whole Serum

Anti-Rat IgG (H+L)Anti-Rat IgG, Fc fragment specificAnti-Rat Whole Serum

Anti-Sheep IgG (H+L)***

Donkey

GoatGoatGoatGoat

RabbitRabbit

Goat

Donkey

GoatGoatGoat

GoatGoatGoat

Rabbit

Anti-Bovine IgG (H+L)***Anti-Bovine Whole Serum***

Anti-Chicken IgY† (IgG) (H+L)

Anti-Dog IgG (H+L)

Anti-Goat IgG (H+L)***

Anti-Goat Whole Serum***

Anti-Guinea Pig IgG (H+L)

Anti-Guinea Pig IgG (H+L)

Anti-Human IgG (H+L)Anti-Human IgG, Fc fragment specificAnti-Human IgG, F(ab')2 fragment specificAnti-Human IgM, Fc5µ fragment specificAnti-Human Whole Serum

Anti-Human IgG (H+L)Anti-Human IgG, Fc fragment specific

301-001-003301-001-001

703-001-003

304-001-003

705-001-003

305-001-001

706-001-003

106-001-003

109-001-003109-001-008109-001-006109-001-043109-001-001

309-001-003309-001-008

RabbitRabbit

Donkey

Rabbit

Donkey

Rabbit

Donkey

Goat

GoatGoatGoatGoatGoat

RabbitRabbit

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, saltfractionated, dialyzed against phosphate buffered saline containing sodium azide, andfreeze-dried. Antisera against whole serums are obtained by immunizing host animals with

whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] arerecommended for bridging PAP (page 75) to primary antibodies.

All products on this page are supplied in 2 ml volumes.



International Distributors

Belgium and LuxemburgELITECH BENELUX BVBAKeistraat 122A9830 St-Martens-LatemBELGIUMTelephone: +32 9 282 05 31Fax: +32 9 282 05 32Email: [email protected] site: www.elitechgroup.com/benelux

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Europe

European Customers:You can order directly fromJackson ImmunoResearch foreuro pricing and faster serviceby contacting JacksonImmunoResearch Europe Ltd,our pan European subsidiary.

Jackson ImmunoResearch Europe Ltd.Unit 7, Acorn Business CentreOaks Drive, Newmarket, Suffolk, CB8 7SY UNITED KINGDOMTelephone: +44 (0) 1638 782616 FAX: +44 (0) 1638 668462Email: [email protected] site: www.jireurope.com


92

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Europe




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94


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United States of AmericaJACKSON IMMUNORESEARCHLABORATORIES, INC.872 West Baltimore PikeWest Grove, PA 19390UNITED STATES OF AMERICATelephone: (800) 367-5296Fax: (610) 869-0171

Email: [email protected]@jacksonimmuno.com

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Africa, Americas, Asia, and Pacific



Trademarks and Licenses

Jackson ImmunoResearch Laboratories, Inc. has trademark rights to the followingnames used in this catalog and elsewhere in its literature: AffiniPure, AffiniSorbed,ChromPure and Biotin-SP (long spacer).

Other trademarks and licenses in this catalog include the following: DyLight™ FluorescentDyes is a trademark of Thermo Fisher Scientific. Jackson ImmunoResearch is licensedby Thermo Fisher Scientific to manufacture and sell conjugates of DyLight FluorescentDyes. Cy™ and CyDye® are registered trademarks of GE Healthcare. JacksonImmunoResearch is licenced by GE Healthcare to manufacture and sell conjugates ofCy2, Cy3, Cy5 and Cy7. Rhodamine Red-X is a trademark of Invitrogen. JacksonImmunoResearch is licensed by Invitrogen to manufacture and sell conjugates ofRhodamine Red-X.

Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp. They areprovided under an agreement between Life Technologies Corp and JacksonImmunoResearch Laboratories, Inc and the manufacture, use, sale, or import of thesedyes is sold pursuant to a license from Life Technologies Corp for use of its fluorescentdye technology. The sale of these dyes or products containing these dyes is expresslyconditioned on the buyer not using them (1) in manufacturing; (2) to provide a service,

information, or data to an unaffiliated third party for payment; (3) for therapeutic,diagnostic, or prophylactic purposes; (4) to resell, sell, or otherwise transfer this productor its components to any third party, or for any other commercial purpose. For informationon purchasing a license to this product for purposes other than research, contact LifeTechnologies Corp, 5791 Van Allen Way, Carlsbad, CA 92008 [email protected].

Cover images reproduced with permission from Professor Samar Hasnain, University ofLiverpool and CSwain Photography.

Design and production: MJWebb Associates Ltd. www.mjwebbassociates.com

Conditions of Sale:

ALL PRODUCTS LISTED IN THIS CATALOG ARE FOR IN VITRO RESEARCH USE ONLY. No product listed in this catalog is a medical device. They are notintended for diagnostic or therapeutic purposes. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. JacksonImmunoResearch cannot be held responsible for patent infringements or other violations that may occur with the use of these products.

Jackson ImmunoResearch makes no implied or other warranty of merchantability or fitness for a particular use and in no event shall Jackson ImmunoResearch be responsible forany consequential or other damages beyond the cost of replacing any product of Jackson ImmunoResearch. Customer agrees to indemnify and hold Jackson ImmunoResearchharmless and to reimburse Jackson ImmunoResearch for all costs related to any claim arising as a result of customer's use of any Jackson ImmunoResearch product contrary tothe terms contained herein or otherwise specified in other documentation.

LABORATORIES, INC.

www.jacksonimmuno.com

Jackson ImmunoResearch Laboratories Inc.872 West Baltimore Pike | West Grove | PA USA 19390

Email: [email protected] Tel: 800-367-5296Fax: 610-869-0171

Documents

Specializing in Secondary Antibodies Conjugates · indicated on the packing list. All claims for missing items must be reported directly to Jackson ImmunoResearch within 5 days after