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Basics of slit lamp microscopy Dr. S S Bhatti www.bhattieye.com

Slit Lamp Basics

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Page 1: Slit Lamp Basics

Basics of slit lamp microscopy

Dr. S S Bhattiwww.bhattieye.com

Page 2: Slit Lamp Basics

The 2 basic parts of the slit lamp biomicroscope are:

• The slit lamp (illumination system)• The biomicroscope

Page 3: Slit Lamp Basics

The illumination system can be

1. Of the Zeiss type2. Of the Haag Streit type

Page 4: Slit Lamp Basics

In the Zeiss type the illumination comes from below

Page 5: Slit Lamp Basics

In the Haag Streit type the illumination comes from above

Page 6: Slit Lamp Basics

In both types of illumination system the Kohler illumination principle is

used:

The filament is imaged on to the objective lens but the mechanical slit is imaged on to the patient’s eye

Page 7: Slit Lamp Basics

The biomicroscope:based on the optics of a compound

microscope

• Two basic types:– The Grenough type– The Galilean changer type

Page 8: Slit Lamp Basics

The Grenough type(Classical Haag Streit)

Flip lever to change magnification

Page 9: Slit Lamp Basics

The Galilean Magnification changer

Knob to change magnification (3 or 5step)

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Galilean magnification changer

Objective

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Magnification can also be changed by changing the eyepiece power

10 X 16 X

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The slit lamp and the biomicroscopeare maneouvred together on a cross

slide by means of a joystick

Page 13: Slit Lamp Basics

The coupling between the slit lamp and the biomicroscope

• This is such as to make the system “parfocal”

• i.e the focus of the slit and the focus of the microscope are at the same point.

• This parfocality may occasionally need to be dissociated as for example in the technique of sclerotic scatter

Page 14: Slit Lamp Basics

The coupling between the slit lamp and the biomicroscope

• This allows both the slit and the microscope to rotate about the point of focus (i.e the eye)

Page 15: Slit Lamp Basics

Dissociation of parfocality can be done in “Haag Streit” type slit lamps by loosening

the sclerotic scatter knob

Page 16: Slit Lamp Basics

This dissociation of parfocality is useful for indirect illumination,

sclerotic scatter and retroillumination

Page 17: Slit Lamp Basics

• The key to successful examination of the anterior segment is knowledge of the various methods of lighting which can be achieved by the slit lamp.

Page 18: Slit Lamp Basics

Diffuse illumination• Not all slit lamps have

this option

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Diffuse illumination for surface details

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Diffuse illumination

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Diffuse illumination

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Diffuse illumination

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Focal broadbeam illumination

Page 24: Slit Lamp Basics

Broad beam

Narrow beam

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Focal broad beam

Busacca’s nodule

on iris

Page 26: Slit Lamp Basics

Knob to widen beam

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Focal slit illumination

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Focal slit illumination

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Retroillumination- against red glow

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Retroillumination- YAG pits on claw IOL

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Indirect illumination(similar to sclerotic scatter)

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Sclerotic scatter

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Knob for sclerotic scatter allows slit beam to be horizontally rocked

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Parfocality of slit and viewing altered for sclerotic scatter

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Sclerotic scatter

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Specular illumination

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Filter turret

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With additional dyes

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Collage

Broad beam slit

Retroilluminationagainst red glow

Retroilluminationagainst lens

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Lens precipitates

Diffuse illumination

Focal illumination

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SPKS- a collage

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Krukenberg spindle

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Traumatic rosette cataract

Page 47: Slit Lamp Basics

Anatomy of the angle

Page 48: Slit Lamp Basics

Normally the angle of the anterior chamber cannot be seen as light from it cannot exit

from the eye due to total internal reflection at the cornea

Page 49: Slit Lamp Basics

A gonioscopy lens allows light from the angle to exit the eye by

eliminating the cornea air interface

Goldman gonioscopy lens

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Direct Direct GonioscopyGonioscopy

Koeppe gonioscopy lensKoeppe Gonioscopy lens

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Angle recession

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Trabecular pigmentation

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• Fundus examination can be done with a slit lamp with the use of ancillary lenses

• Ancillary lenses are required to neutralize the refractive power of the cornea .

Page 54: Slit Lamp Basics

Use of the short reflex mirror is recommended for posterior segment examination because the upward projection of the long mirror blocks one of the eye pieces when the illumination is kept at a small 3-5

degree angle from the binocularHowever, the illumination beam column must then be tilted else the illumination beam will fall partly outside the mirror reducing the illumination entering the eye

Page 55: Slit Lamp Basics

Broad beam

Narrow beam

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Some contact Fundus slitlamplenses

Mainster standard

MainsterHigh Magnification

Mainster PRP (widefield for panretinalphotocoagulation)

Page 57: Slit Lamp Basics

Fundus view with slitlamp and Mainster contact lens

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Slit lamp examination with a Volk quadraspheric contact lens (dislocated crystalline lens)

Page 59: Slit Lamp Basics