Upload
camilla-pugh
View
39
Download
0
Embed Size (px)
DESCRIPTION
Serial Dilutions. Get 2 x 3 inch jewelry bags jewelry supply store. See product # 52-002 on second table @ http://www.aajewelry.com/templates/product_list_dropdown_pix.aspx?GroupGuid=5523. Staple across the bag such that there is space for roots to grow. - PowerPoint PPT Presentation
Citation preview
Serial DilutionsSerial Dilutions
Get 2 x 3 inch jewelry
bags jewelry supply store. See
product # 52-002 on second table @
http://www.aajewelry.com/templates/product_list_dropdown_pix.aspx?GroupGuid=5523
Cut piece of paper towel and insert into bag
Staple across the bag such that there is
space for roots to grow
Add 10 to 15 seeds to each
bag
0.2 g/mL NaCl0.2 g/mL NaCl
Stock SolutionStock Solution
Make a 0.2-g/mL NaClNaCl STOCKSTOCK solutionsolution
- Add 2 g of sodium chloride to beaker and add water to 10 mL
Get as many test tubes (minus two controls) as you have plastic
jewelry bags for your experiment
Add 5 mL of water to each of you test tubes
Add 5 mL of Stock Solution to
first test tube.
Mix the first Tube
Take 5 mL of first tube and add to second
tube
Mix
Repeat and remove 5 ml
from last tube and discard
Using a 5mL disposable pipette 1.Add 3 mL from stock to a bag
2.Add 3 mL of water to another bag 3.Add the 3 mL from each test tube to
its respective bag
Stock SolutionStock Solution
Stoc
k
WaterWater
Wat
er
0.1 g/mL 0.1 g/mL SolutionSolution
0.1
g/m
L
0.05 g/mL 0.05 g/mL SolutionSolution
0.5
g/m
L
EtcEtc..
Let Grow in Dark for ~ 1 week