Sequencing concepts

ChemStation - Level 1 - Training

April 04, 2002 Agilent Restricted

Building a Sequence for Automated Analysis

1. Go to the Method and Run Control view.

2. From the Sequence menu, select New Sequence.

3. Edit the Sequence Parameters.

4. Edit the Sequence Table.

5. Select a Sequence Output.

6. Save the Sequence

7. Run the Sequence form the RunControl menu or the Sequence Table.

April 04, 2002 Agilent Restricted

Sequence Parameters

April 04, 2002 Agilent Restricted

Standard ALS Sequence Table

Additional Columns inSequence Tables:

• Sample Amount• ISTD Amount• Multiplier• Dilution • Data file• Inj Volume

April 04, 2002 Agilent Restricted

WPS Sequence table

The set up sequence starts with well plate 1 at location A1 to B4 and ends on plate 2 where samples from B5 to G10 are analyzed.

Sample table set up screen

Next Previous Text Home

April 04, 2002 Agilent Restricted

Insert Vial Range Standard ALS

April 04, 2002 Agilent Restricted

Fill in Sequence Table Using Sequence Table Fill Down - Standard ALS

1. Insert Values2. Apply

April 04, 2002 Agilent Restricted

WPS Sequence table

The set up sequence starts with well plate 1 at location A1 to B4 and ends on plate 2 where samples from B5 to G10 are analyzed.

Sample table set up screen

Next Previous Text Home

April 04, 2002 Agilent Restricted

Partial Sequence Preview

April 04, 2002 Agilent Restricted

Save the Sequence File

Save Current Sequence

April 04, 2002 Agilent Restricted

Start a Sequence

•RunControl menu•Start button•F6•Sequence Table

April 04, 2002 Agilent Restricted

Sequence Data Files

Format for Automatic Injection: 003-0101003 Vial Number01 Sequence Line01 Replicate

PrefixPrefix01Prefix02, etc. for a total of 8 characters

April 04, 2002 Agilent Restricted

Sequence Sampling Diagram

Shows sequence tray set-up and sequenceprocessing status. Click to set-up sequence.

Standard ALS

Wellplate Sampler

April 04, 2002 Agilent Restricted

Lab Time !!!!! Lab 4- Sequence