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Selected and Differential Media
Define
Selective Media Differential Media Enriched Media
Blood Agar Plate (BAP)
Differential Enriched Use
Growth Hemolysis
Alpha Beta Gamma
C-CNA
Selective Differential Results
Gram (+) Colistin Naldixic acid
Blood Hemolysis
MSA
Selective Differential Results
Halotolerant (7.5% NaCl) CH2O: Mannitol pH
phenol red Fermentation
Acid Yellow
MAC
Selective Differential Results
Gram (-) CV Bile Salts
Lactose pH indicator
Neutral red indicator Ferment = pink/red No acid = colorless
EMB
Selective Differential Results
Gram (-) Eosin dye MB dye
Lactose Some acid = pink Large acid = green No acid = colorless
Set Up
One plate for each group Label quadrants as lab
book indicates for each plate
Cultures on cart Anaerobic Gas Pack
BAP C-CNA
All others aerobic 37°C
BAP results
Sheep blood enriched Growth most all microbes Fastidious Differential
GP Hemolysins Hemolysis types
Gamma Alpha Beta
Hemolysis
No hemolysis
C-CNA results
Selective Growth GP
Colistin Nalidixic acid
Differential Hemolysis
MSA results
Selective 7.5% NaCl Staphylococcus spDifferential Mannitol fermentation phenol red yellow
MAC results
Selective: GN CV Bile Salts
Differential Lactose fermentation NF = colorless Coliforms
Neutral red pink
EMB results
Selective: GN Dyes
Eosin MB
Differential Lactose fermentation NF = colorless Acid production
Small = pink Large = metallic green