Clinical Microbiology

• Growth Dependent ID• Selective Media• Differential Media• Enrichment Culture• Transport Media• Blood Agar• Chocolate Agar

Diagnostic Media Products

• Fermentation Media

• Diagnostic tests

• API 20E strips

• Enterotube

Anaerobic Cultures

• Anaerobic jars

• Reducing agents

• Anaerobic Hoods

• Safety Hoods

• BL1, BL2, BL3, BL4

Antimicrobial Drug Sensitivity

• MIC –minimum inhibitory concentration

• Disk Diffusion

• Tube Dilution

• Antibiogram

Immunodiagnostics

• Antibody Titers• Skin Tests• Coated-particle

Agglutination• Fluorescent

Antibodies

Monoclonal Antibody

• Polyclonal Antibodies• Hybridoma• Myeloma

Antigen-Antibody Reactions

• Neutralization• Precipitation• Agglutination

Enzyme-linked Immunosorbent Assay

ELISA

Direct – Antibody Bound to Well

Indirect – Antigen Bound to Well

Enzyme Magnify by Repeatedly Turning Substrate to Product

Colorless Substrate Colored Product

Direct

ELISA

Indirect

ELISA

Immunological Tests

• ELISA uses

• HIV, Drugs, Viruses

• Radioimmunoassay

• Immunoblot

Nucleic Acid Probe

Hybridization

Filter or Dipstick

Reporter

Polymerase Chain

Reaction – PCR

Nucleic Acid Based Methods

Clinical Micro Word List

Growth Dependent ID

Anaerobes

Selective Media

Differential Media

Antimicrobial Drug Sensitivity Immunodiagnostics

Antibody Titers

Skin Test

Monoclonal Antibodies

Coated-Particle Agglutination

Fluorescent Antibodies

Enzyme-Linked Immunosorbent

Assay – ELISA

Direct – Indirect

Radioimmunoassay

Immunoblot

Nucleic Acid probes

Polymerase chain Reaction – PCR

Diagnostic Virology

Electron Microscope