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PREVENTING & TREATING THE LEADING CHILDHOOD EPIDEMIC:

AUTISMFounder, Hope For AutismPresident, Heart For Autism

Dr. Renee Hunter

Pediatric Health

"There are those who would predict that, in this generation of children, the parents are going to be the first to outlive this generation of kids if this trend isn't reversed,"

-McCrindle, an author and professor of pediatrics at the University of Toronto

May/June 2011 Academic Pediatrics

Stats - 2007 National Survey of Children’s Health

Parents of about 82,000 American children

%54.1%Obese, Developmental Delay or 1 of 20 Chronic Illnesses

California Health and Human Services AgencyASD now is more prevalent than

Type I diabetes (CDC, 2005)

Down syndrome (National Center for Birth Defects, 2005)

Childhood cancer (United States Cancer Statistics Workgroup Childhood cancer (United States Cancer Statistics Workgroup, 2005)

Cerebral palsy

Soon projected to exceed epilepsy

“Autistic Spectrum Disorders: Changes in the California Caseload, An Update June 1987 June 20007.” Cavagnaro, Andre T., California Health and Human Services

Agency. State of California 2003 survey of developmental disabilities.

For perspective…

A couple decades ago,

autism affected only

March 2013 National Survey of Children’s Health

Human Resources and Services Administration (US Department of Health and Human Services)

Nearly 82,000 children

Odds of a parent having a child diagnosed with ASD is…

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ADDM

Autism and Developmental Disabilities Monitoring Network

Funded by CDC to estimate the number of children with autism spectrum disorders p(ASDs) and other developmental disabilities

New data released in 2012 tracked only 14 states and only parts of some of those states.

Currently, the number of states has dropped to 12 in the ADDM network.

ADDM – Bad Science

CDC removed states with high and/or rising rates. Following the 2002 report, NJ, UT, WV, and AR were all

removed from ADDM. These 4 states were ranked #1, #3, #5 and #6, respectively. This seems to be systematic purging of high prevalence states.

Thi t l l d d t t ith hi h t i 2002 it This purge not only excluded states with high rates in 2002, it also excluded 2 of the 3 states with the largest rates of increase between 2000 and 2002: WV with a 39% increase in just 2 years, and NJ with a 7% increase.

In 2002, CDC added AL to the ADDM. AL is one of the bottom 10 states in the IDEA rankings, and at the time it entered had the lowest rate in the ADDM network. CDC retained AL in the network for all subsequent reports.

2012 – CDC Official Statistics

The prevalence of autism is rising at about 12% a year:

The number of children with autism will double every 6 years. In 5 years, there will be at least one child with autism in every 5 y y

classroom in America. At current rates, 45, 454 children will be diagnosed with

autism. At this rate, in 5 years, 80,000 children per year will be

diagnosed with autism.

Can anyone with a conscience claim that this isn’t an epidemic?

2014 – Official Statistics

Prevalence is 1 in 68 births1

Fastest-growing developmental disability; 1,148% growth rate.4

10 – 17 % annual growth.57 g $60 billion annual cost.6

1. “Prevalence of Autism Spectrum Disorders — Autism and Developmental Disabilities Monitoring Network, 11 Sites, United States, 2010.” Department of Health and Human Services, Centers for

Disease Control and Prevention. Morbitity and Mortality Weekly Report, 28 March 2014

2. “Autistic Spectrum Disorders: Changes in the California Caseload, An Update June 1987 June 20007.” Cavagnaro, Andre T., California Health and Human Services Agency. State of California

2003 survey of developmental disabilities.

3. Autism Society estimate based on 2003 US state educational data.

4. Autism Society estimates based on UK study by Jarbrink K, Knapp M, 2001, London School of Economics: “The economic impact on autism in Britain,” Autism, 5 (1): 7-22.

Constant Controversy

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Change in Diagnostic Criteria?

Original diagnosis was the most broad… Diagnosis has become more specific. (Requires more to label a child “Autistic”).

No change in criteria since 1994

Better awareness/diagnosis has had only a modest effect (per study by MIND Institute).

Is it Possible?

If the rates have always been 1 in 50…

Doctors previously missed 98.5% of cases

Doctors are now over 7000% better at diagnosing

If Rates Have Not Increased…

We are MISSINGOVER 2 Million undiagnosed

adults

At a minimum estimated cost of$3.2 - $5 million for care

NIH Research Funding 2011

$169 million on Autism – which affects

45, 454 US babies each year

$79 million on Cystic Fibrosis – which affects 1,081 US babies each year

$228 million on Pediatric AIDS – which affects 13,333 US babies each year

$170 million on Pediatric cancer – which affects 800 US babies each year

Genetic or Environment?

Maybe genetic susceptibility...

But NOT strictly genetic because there is no such thing as a genetic epidemic!

Genetic diseases always remain relatively constant.

Gene pool changes slowly.

Autism MUST have an ENVIRONMENTAL COMPONENT!

Genetic or Environment?

Study twins (both identical and non-identical) where at least one of the pair has autism.

Assess how often both twins receive a diagnosis of autism. Look at prevalence of both autistic in identical twins, who

share 100 percent of their genes. Compare to fraternal twins, who share around 50 percent of Compare to fraternal twins, who share around 50 percent of

their DNA. Can determine the degree to which autism is inherited, or

genetic. Shows how environmental influences add to the risk of

autism.

Genetic Heritability and Shared Environmental Factors Among Twin Pairs With Autism,Arch Gen Psychiatry

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Genetic or Environment?

The largest twin study to date found that autism risk is

55-58% environmental55 58% environmentaland only 37-38% genetic.

Genetic Heritability and Shared Environmental Factors Among Twin Pairs With Autism, Arch Gen Psychiatry

2009 Research Funding

Funding for genetic causation research outweighed environmental causation research

approximately 6 to 1.

We will not find answers looking in the wrong place.

Molecular Biology

"The Central Dogma" states that biological information is transferred sequentially and only in one direction (from DNA to RNA to proteins). L d t b li f i b l t d t i i hi h Leads to belief in absolute determinism, which means that health and disease are results of your genetic code, which you were born with.

Science is proving this theory FALSE. Our responses to our environment determine

the expression of our genes.

Human Genome Project

13 years of research that ended in 2003

Goal was to map out all human genes and their interactions

This would serve as a basis for curing all This would serve as a basis for curing all disease

Discovered the human body consists of far fewer genes than previously believed

Genes do not operate as previously predicted

Epigenetics

Changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence

Modifications to the genome that do not involve a change in the nucleotide sequencechange in the nucleotide sequence

Non-genetic factors cause the organism's genes to express themselves differently

Some researchers, like Randy Jirtle, PhD, of Duke University Medical Center, think epigenetics may ultimately turn out to have a greater role in disease than genetics.

Epigenetics

The human genome is the blueprint for the body.

The epigenome—the way the genome is marked and packaged inside a cell’s nucleus—tells a cell which of the many sets of instructions on that blueprint to follow, which ones to ignore, and which ones to follow over and over again.

If the genome is the blueprint, the epigenome is the contractor directing how the walls and windows are made, and whether the plumbing is correctly installed.

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Epigenetics

Researchers at Duke University Medical Center showed that the activity of a mouse's genes can be influenced by food supplements eaten by its mother just prior to, or during, very early pregnancy (New Scientist, 9 August 2003, p 14).

Moshe Szyf, Michael Meaney and colleagues at McGill University in Montreal, Canada, showed that mothers could influence the way a rat's genes are expressed after it has been born. If a rat is not licked, groomed and nursed enough by its mother, chemical tags known as methyl groups are added to the DNA of a particular gene.

What Controls Genetics?

Toxic exposures

Exposure to sunlight

Trauma

Nutrition Nutrition

Exercise

Everything you put in your body

Thoughts and belief systems

If Autism is mainly If Autism is mainly ENVIRONMENTAL…

Th_n @utism ][n \_

PREVENTED

An ounce of prevention is worthis worth

a pound of cure.-Benjamin Franklin

Flu Deaths 1999, under 5 yrs.

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Flu Deaths 2000, under 5 yrs. Flu Deaths 2001, under 5 yrs.

Flu Deaths 2001 & 2002, under 5 yrs.

2002 Flu Shot Mandate for Children,

Flu Deaths 2003, under 5 yrs.

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Flu Prevention Over-rated?

Whatever CAUSES Autism…

Something that is NEW within the past few decades.

Something that affects children consistently across the country. (Ex - Not just near industrial plants)

Something that affects children equally in all economic classes. (Not just children in poverty or from wealthy families)

Something that is not affected by amount of exercise or nutrition.

Something that affects BOYS at a rate of 4-5:1.

What is AUTISM?

Was originally considered a mental illness caused by inadequate parenting

Dr. Bernard Rimland, founded National Society for Autistic Children (Autism Society of America) and the Autism Research Institute (ARI)( )

The ARI, which has become the foremost autism research organization in the world today.

The ARI has identified a long list of biological and environmental factors that either cause or contribute to autism

A comprehensive range of effective treatments.

He brought full recovery for at least 1,100 children

Dr. Bernard Rimland

Autism is… “A biological disorder – a problem within the body, not the mind and certainly not the mother.”

Autism affects critical parts of metabolism, with symptoms in the immunological, gastrointestinal, toxicological and neurological systems (Jepsontoxicological, and neurological systems (Jepson, 2007b).

Testing often reveals mitochondrial dysfunction, heavy metal toxicity, nutrient deficiencies, food and inhalant allergies, gluten and casein intolerance, immune system deficiency, intestinal dysbiosis, brain and gut inflammation and more…

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State of Our Environment

More than 80,000 new chemicals now circulate in consumer products since the rise of the petrochemical industry.

Very few tested for toxicity Human fat tissue sampled in the United States has p

shown 700 chemical contaminants [1]

Onstot, J. et al. “Characterization of HRCG/MS unidentified peaks from the analysis of human adipose tissue.” Vol 1. Technical

Approach, US EPA Office of Toxic Substances (560/8-87-002a); 1987

Chemicals

“With chemicals, it’s shoot first and ask questions later.”

— Al Meyerhoff, former attorney for the

Natural Resources Defense Council

Environmental Working Group

Found that blood samples from newborns contained an average of 287 toxins, including mercury, fire retardants, pesticides, and Teflon chemicals.

Of the 287 chemicals EWG detected in umbilical cord blood, it’s known that: 180 cause cancer in humans or animals 217 are toxic to your brain and nervous system 208 cause birth defects or abnormal development in

animal tests

AUTISM PREVENTION:

ELIMINATEELIMINATE CHEMICALS

Autism Prevention: Eliminate Chemicals

Phthalates (pronounced THAL-LATES) –major environmental group of toxins linked to abnormal sex development, cancer, neurological disordersneurological disorders

Pesticides – Organophosphates, organochlorines and other organic solvents used to kill insects in the home or fields.

Phthalates

Family of chemicals used in many plastics worldwide to improve flexibility and to bind fragrance.

Also provide the flexibility in vinyl products, including upholstery, packaging and wall and fl ifloor covering.

Adults and children are exposed to phthalates through everyday contact with these products as well as through contact with indoor air and dust.

When chewed or sucked in the course of normal play, phthalates leach from toys into children’s mouths.

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Phthalates

Premature birth Reproductive defects Early onset puberty Cancer Autoimmune disorders like lupus Autoimmune disorders like lupus Organ damage in animal trials Neurological disorders AUTISM

Europe has outlawed two major types of the compounds in cosmetics and baby toys.

Phthalates & Autism

Phthalates, BPA linked to atypical childhood social behaviors. NeuroToxicology, Volume 32, Issue 2, March 2011, Pages 261–267

This study investigates prenatal exposure to two ubiquitous endocrine disruptors, the phthalate esters and bisphenol A ( ) d l b h l f d l(BPA), and social behavior in a sample of adolescent inner-city children. Prenatal phthalate exposure was associated with childhood social impairment in a multiethnic urban population. Even mild degrees of impaired social functioning in otherwise healthy individuals can have very important adverse effects over a child's lifetime. These results extend our previous finding of atypical neonatal and early childhood behaviors in relation to prenatal phthalate exposure.

Phthalates & Autism

Di-(2-ethylhexyl) phthalate and autism spectrum disorders. ASN Neuro. 2012; 4(4): e00089.

Our findings demonstrate for the first time an association between phthalates exposure and ASDs, thus suggesting a previously unrecognized role for these ubiquitous environmental contaminants in the pathogenesis of autism.

Phthalate Exposure

After shave lotions Aspirin Bath preparations Cosmetics Detergents

d i d i h

Insect repellents Nail extenders Nail polish Nail polish remover Skin care products

dh i Foods microwaved with plastic covers

Oral pharmaceutical drugs IV products prepared in

plastic bags Eye shadows Hair sprays Insecticides

Adhesives Perfumes Phthalates are commonly

added to pharmaceuticals to provide a time release mechanism.

Pesticides

Children exposed, either before or after birth, had more than twice the risk of developing autism.

In CA agricultural areas, children born to mothers living within 500 meters of fields where organochlorine pesticides were used were more than 6 times more likely to develop autism.

ASD risk increased with the poundage of organochlorines applied and decreased with distance from field sites.

Autism Prevention Strategies

Reduce exposure Organic, natural home and beauty products Use proper ventilation upon entering automobile Eat organic foods Avoid OTC and prescription meds

Aid body detoxification Infrared sauna (prior to pregnancy) Liver cleanse / detox Support gastrointestinal health

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AUTISM PREVENTION:

AVOID ULTRASOUNDAVOID ULTRASOUND

Autism Prevention: Avoid Ultrasound Prenatal Ultrasound – NO conclusive studies showing

safety

Early studies showed that subtle effects of neurological damage : an increased incidence in left-handedness in boys (a marker for brain problemshandedness in boys (a marker for brain problems when not hereditary) and speech delays.

As early as 1979, studies questioned the use of ultrasound and its potential effects on DNA and growth patterns.

In 1987, a published study reported diagnostic levels of ultrasound disrupted myelination. The ultrasound intensities used in this study were consistent with those used for human imaging.

Ultrasound

1993 – study concluded that children exposed to ultrasound in utero were twice as likely to develop delayed speech

2004 FDA warning, "ultrasound is a form of energy, and even at low levels, laboratory studies have shown it can produce physical effect in tissue, such as jarring vibrations and a rise in temperature.“

August 2006 -Yale School of Medicine's Department of Neurobiology: pregnant mice underwent various durations of ultrasound. The brains of the offspring showed brain injury damage consistent neurological changes seen in autism.

The research also implicated ultrasound in neurodevelopmental problems in children, such as dyslexia, epilepsy, mental retardation and schizophrenia

Damage to brain cells increased with longer exposures

Ultrasound

Temperature increases can cause significant damage to a developing fetus's central nervous system (CNS) and birth defects in offspring.

An extensive review of literature on maternal hyperthermia in a range of mammals found thathyperthermia in a range of mammals found that CNS defects appear to be the most common consequence of hyperthermia in all species

2001 Cornell University research: brain development proceeds in the same manner "across many mammalian species, including human infants."

AUTISM PREVENTION:

PROMOTE GI HEALTH

Autism Prevention - GI Health

Gastrointestinal problems are extremely prevalent in children with autism.

Vaginal delivery plays a key role in the infants first bacterial exposure and the gastrointestinal development that follows.

Baby inherits mother’s flora (vaginal canal, breastfeeding, etc..)

Optimizing maternal GI health and addressing problems can enhance the likelihood that the infant will not be prone to GI problems.

Buie, Timothy, et al., Evaluation, Diagnosis and Treatment of Gastrointestinal Disorders in Individuals with ASDs; A Consensus Report and Recommendations for Evaluations and

Treatment of Common Gastrointestinal Problems in Children with ASD, Pediatrics, 1-4-2010

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Patient Strategy – GI Health

Reduce or eliminate: Sugars and grains Chemical preservatives Processed foods Antibiotic use

T t f i t ti l th h t l b t i Test for intestinal pathogens such as yeast, non-commensal bacteria and parasites Can be treated with special diets and supplements

Test for and avoid all food allergens/sensitivities

Probiotics

The importance of a vaginal delivery and breastfeeding cannot be stressed enough.

AUTISM PREVENTION:

THE MERCURY CRISIS

Autism Prevention: The Mercury Crisis

The EPA estimated that 1 in 6 children

(630,000 children per year) born in US have unsafe blood levels of mercury at birth.

(Mahaffey, 2004)

These levels are high enough to cause a decline in IQ at an estimated annual cost of

$8.7 billion. (Trasande, 2005)

Sources of Mercury Exposure

Air“For every 1000 lbs environmentally released Hg

there is a 43% rise in special education services and a 3.7% rise in autism”

U i it f T H lth S i C t / / 8-University of Texas, Health Science Center 4/4/08

Power plants and waste disposal plants emit 50 tons of Hg into air each year rain fills streams, rivers, lakes, oceans…. And the water table eventually!

One gram of mercury can make the fish in a 20-acre lake unfit for consumption for a year.

Sources of Mercury Exposure

High Fructose Corn Syrup (HFCS) Hg was found in 50% of high fructose corn syrup Detected in 1/3 of 55 popular brand-name foods Americans consume 12 teaspoons of HFCS a day

S f d Seafood Plankton small fish big fish (tuna 100X Hg of

small fish) Ingested methyl Hg gut blood organs (brain

highest amount) “It can destroy the biological function of any protein it

binds to” , Boyd Haley PhD

Women who ate 9 servings of fish per month had 7X the Hg levels of non-fish eaters. (EPA)

Sources of Mercury Exposure

Dental Amalgams Comprised of 50% Hg Release vapor…80% absorbed into body 1-10 mcg/day absorbed depending on number of

amalgamsg “Brain Hg increased 17% for each increment of

amalgam surface” (also kidney, thyroid and pituitary)Am J Forensic Med Pathol (2006) 27:42-45

Vaccines Thimerosal -50% ethylmercury

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Mercury’s Affect on the CNS

Decreases nerve growth factor Destroys astrocytes, neurons, mitochondria Alters key digestive enzymes Disrupts uptake/release of neurotransmitters

C id ti Causes oxidation Disease Premature aging Inflammation, including inflammation of the lining of your

blood vessels. When this occurs, your LDL levels increase as your body attempts to "patch" those damaged vessel walls with cholesterol. LDL is a carrier of cholesterol. This is why people with mercury toxicity have damaged blood vessels, and elevated cholesterol and LDL levels.

Mercury Toxicity

All of the diagnostic criteria needed for a diagnosis of autism were also seen in Hg toxicity.

Using Hg in rats, the same brain lesions as in g g ,80% of Alzheimer’s patients can be produced.Dr. Boyd Haley

Damages mitochondria American Journal of Biochemistry and Biotechnology 4 (2): 208-217,

2008

Boys at Highest Risk

Autism rates 5:1 (boys : girls)

Girls have a greater intracellular glutathione level less oxidative stress and cellularlevel… less oxidative stress and cellular damage Rush J.W., Sandiford S.D.

Testosterone potentiates Hg toxicity

Med hypothesis 2005:64(5)

Mercury Poisoning at Birth?

1. Amalgam Fillings: What happens when a dentist removes a silver filling?

Rh G Sh t M i f2. RhoGam Shot: May contain 25 mcg of Thimerosal. Given up to 3 times during pregnancy

3. Mercury rapidly crosses the placenta and into the fetus where the fetus actually accumulates mercury at a much higher rate than the mother and typically has blood levels 70% higher than those found in the mother at the time of birth. (EPA)

Mercury’s Affect

Decreases nerve growth factor Destroys astrocytes & neurons Alters key digestive enzymes Disrupts uptake/release of neurotransmitters

C id ti Causes oxidation Disease & Premature aging Inflammation, including inflammation of the lining of your

blood vessels. When this occurs, your LDL levels increase as your body attempts to "patch" those damaged vessel walls with cholesterol. LDL is a carrier of cholesterol. This is why people with mercury toxicity have damaged blood vessels, and elevated cholesterol and LDL levels.

Damages mitochondriaAmerican Journal of Biochemistry and Biotechnology 4 (2): 208-217, 2008

Mercury’s Affect (As seen in Autism)

Induces glutathione depletion (James et al., 2005)

Increases oxidative stress, and apoptosis in these cells (Makani et al., 2002).

Is toxic effects on brain cells (Baskin et al., 2003)( , 3)

Inhibits glutamate transport (Mutkus et al., 2005)

Affects nerve differentiation (Parran et al., 2005)

Induces immunoproliferation and formation of autoantibodies to fibrillin proteins (Havarinasaband Hultman, 2005)

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Mercury Exposure & Autism

Every major symptom of autism documented in cases of mercury poisoning

Biological abnormalities in autism are very similar to the side effects of mercury poisoning itself (Bernard et al., 2001): psychiatric disturbances (impairments in sociality psychiatric disturbances (impairments in sociality,

stereotypic behaviors, depression, anxiety disorder, etc) increased incidences of allergies and asthma increases in the presence of IgG autoantibodies against

brain and myelin basic proteins reductions in natural killer cell function increases in neopterin levels (indicative of immune

activation).

Mercury Exposure & Autism

Autistic brains show neurotransmitter irregularities that are virtually identical to those arising from mercury exposure changes in serotonin and dopamine concentrations elevated epinephrine and norepinephrine levels in the plasma and

brain elevated serum glutamate levels

t l h li d fi i i th hi (B d t l ) acetylcholine deficiency in the hippocampus (Bernard et al., 2001).

First use of Thimerosal in vaccines in the 1930s closely preceded the discovery of autism in 1943. (Kanner, 1943)

Due to the extensive parallels between autism and mercury poisoning, the likelihood of a causal relationship is great.

Mercury & Amalgam Fillings

Amalgam Fillings: According to the FDA - 50% mercury by weight

World Health Organization - Human studies and experiments in laboratory animals indicate that d l l ib i ifi ldental amalgam contributes significantly to mercury body burden in humans who have amalgam fillings

Of the mercury vapor, some is exhaled, some is inhaled into the lungs and absorbed into the blood, some is retained in the vapor form in the saliva and swallowed together with amalgam particles, and some is oxidized to an ionic form and spit from the mouth or swallowed.

Thimerosal (50% ethylmercury)

50% mercury by weight

Mercury is almost the deadliest element on earth, second only to plutonium.

Nervous system and brain damage are both proven ff t f h th th t i i i h l deffects of mercury exposure whether the toxin is inhaled,

eaten or even placed on the skin.

Developed by Eli Lilly in 1929

Department of Defense during WW2 said Eli Lilly must label it “Poison”.

1948 - one part per million can cause neurological injury.

Thimerosal (50% ethylmercury)

Accumulates in the brain Neurons are observed to disintegrate in its presence Causes chromosomal changes Also associated with neurological disorder,

demyelinating diseases, gut diseases and visual damage y g , g g(Merck)

The dose the Environmental Protection Agency (EPA) deems allowable is 0.1 mcg per kilogram(2.2 lbs) per day.

1980s and 1990s - babies were injected with mercury levels that exceeded the federal safety levels for dietary mercury by 87 times.

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Paul Thorsen

A psychiatrist, not a research scientist or toxicologist. Responsible for a majority of the thimerosal studies

that claimed to show Thimerosal’s safety. The CDC paid Thorsen and his research staff millions

of dollars to churn out research papers many of themof dollars to churn out research papers, many of them assuring the public on the issue of vaccine safety.

In 2010, was indicted on fraud charges for falsified documents and, in violation of university rules, accepting salaries from both the Danish university and Emory University in Atlanta.

He then has vanished along with almost $2 million in taxpayer dollars that he had supposedly spent on research.

Hasn’t Thimerosal Been Removed?

Congressional Hearings in 2000 – 2004: public pressure caused vaccine producers to finally take action.

The amount of Thimerosal was reduced in several vaccines leaving a “trace” amount. g

“Trace” amounts can still be in excess of one part per million.

Numerous vaccines in a single day,

trace + trace + trace = a toxic exposure.

What is a “trace” of Thimerosal?

The US EPA and FDA recommend 1 ppm as the official reference dose for methyl mercury in the United States. The 1-ppm value is also used with regard to total mercury exposure, according to the Joint Expert Committee on Food Additives (Dufault et al., 2009).

b l d d h d 200 ppb mercury in liquid waste renders it toxic hazard

25,000 ppb is in infant flu vaccine

50,000 ppb is found in regular flu vaccine recommended for children and adults

Thimerosal in Vaccines

Numerous vaccines still contain up to 25 micrograms of Thimerosal.

The vaccines containing the highest levels of Thi l i l d i l ti i t th flThimerosal include inoculations against the flu, Hepatitis A, Hepatitis B, Meningococcal disease, Japanese encephalitis, and Pertussis.

Many combination vaccines like the DTaP, also contain Thimerosal.

Medical “Explanation” that Defies LOGIC

Health officials say Thimerosal is not dangerous because we are all

exposed to higher levels eating fish…exposed to higher levels eating fish…

Mercury we ingest when eating fish is methylmercury.

Thimerosal is ethylmercury.

National Institutes of Health 2005

Ethylmercury is more toxic than methylmercury because it crosses the blood-brain barrier quicker and converts to inorganic mercury.g y

This inorganic mercury is most difficult to excrete and stays in the brain longer and in higher levels.

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No Comparison

Gastrointestinal system is equipped to chelateheavy metals and toxins from the body.

Wh i j t b ll l When you inject, you bypass all normal protection, leaving all organs and tissues vulnerable.

Injecting Vs. Ingesting

“…the route of exposure is absolutely critical. This is a key principle of toxicology and it never ceases to amaze me that the argument equating dietary to injected aluminum (or mercury) is made at all. Most dietary aluminum (or mercury) will be excreted Injected aluminum is not easily removedexcreted. Injected aluminum is not easily removed, hence one reason it is used as an adjuvant in the first place.”

- Chris Shaw, PhD, University of British Columbia. Shaw has stunning credentials that make it clear he is overwhelmingly qualified to study this topic. He's a professor in the Department of Ophthalmology and

Visual Sciences at the UBC and he holds cross appointments with the Department of Experimental Medicine and the Graduate Program in Neuroscience. He is the author of more than 100 peer reviewed

articles as well as numerous book chapters and special reviews and has edited four books on neuroscience themes. He has done extensive work on the link between Al and ALS, Alzheimer's, and Parkinson's.

- Now he's also addressing how exposure to Al could be linked to the autism epidemic.

Thimerosal Studies

2003 ‐ Archives of toxicology

"In conclusion, Thimerosal induced strong effects in the cytochalasin B in vitro [outside the living organism] micronucleus test in human lymphocytes ... Since Thimerosal was repeatedly shown to be genotoxic[damaging to DNA] in vitro and in vivo [inside the living organism], there is reason for concern about its widespread use "is reason for concern about its widespread use.  

2004 ‐ Toxicology

"Both Thimerosal and methyl mercury increased the [Ca2+]i and oxidative stress in cerebellar granule cells…action on brain neurons dissociated from 2‐week‐old rats under the in vitro conditions is similar to that of methylmercury." 

Thimerosal Studies

1999 - Division of Epidemiology and Surveillance, Vaccine Safety and Development Branch, National Immunization Program, Centers for Disease Control and Prevention

“Increased risk of developmental neurologic impairment after high exposure to thimerosal-containing vaccine in first month of life.”

Thimerosal Studies

2007 - Journal of Toxicology and Environmental HealthA subsequent study using cultured human neuron and fibroblast cell lines similarly showed that low micromolar concentrations of Thimerosal induced DNA strand breaks, caspase-3 activation, membrane damage and cell death."

“Reforms in the manufacture and the licensing of vaccines and other drugs, which should have been accomplished proactively, had anyone properly assessed their mercury

content, must now be conducted, reactively, under significant systemic stress With no warning recall or

"The high order of toxicity from Thimerosal and its ethyl mercury breakdown product has been known and published for decades. Nonetheless, Thimerosal remains in the drug supply, especially in various vaccines manufactured both for the United States and globally. The ubiquitous and largely unchecked place of Thimerosal in pharmaceutical products, therefore, represents a medical crisis in the modern day.”

significant systemic stress. With no warning, recall, or ban of mercury in vaccines and other drugs as of yet,

the victim of this mandated, unwarranted, and massive mercury exposure is still an unsuspecting public, and most especially its unborn and newborn

children."

Thimerosal Studies

2008 - Neuroendocrinology Letters

"Thimerosal has been recognized by the California Environmental Protection Agency, Office of Environmental Health Hazard Assessment as a developmental toxin. This i li th t Thi l d bi th d f t l bi thimplies that Thimerosal may produce birth defects, low birth weight, biological dysfunctions, or psychological or behavior deficits that become manifest as the child grows. Maternal exposure during pregnancy may disrupt the development or even cause the death of the fetus... It is clear from these data that additional research should be undertaken in the context of evaluating mercury-associated exposures, especially from Thimerosal containing Rho(D)-immune globulins administered during pregnancy.

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Thimerosal Studies

2009 - Behavioral and Brain Functions

"A disruption of the GSH (glutathione) system by mercury leads to GSH depletion and cell destruction. .. GSH depletion is linked to several neurodegenerative disorders."

2009 NeuroToxicology 2009 - NeuroToxicology

“….None-the-less, the developing brain is considered the most vulnerable organ to mercury exposure, and experimental studies suggest that the brainstem - whose function is central to the reflexes described herein …it is possible that exposure to ethyl mercury may adversely affect the emerging brainstem function ...

Thimerosal Studies

2010 – Child Health SafetyThe United States Government and Dr. Geberding, Director of Vaccines at Merck & Co., Inc. say that autistic conditions can result from encephalopathy following vaccinationfollowing vaccination.

2013 - Translational Neurodegeneration

“A two-phase study evaluating the relationship between Thimerosal-containing vaccine administration and the risk for an autism spectrum disorder diagnosis in the United States.”

MERCURY EXPOSURE FROM VACCINES

Until 2003 –

By 6 months a fully vaccinated infant had received:

3 DPT 75 mcg

3 Hib 75 mcg

3 Hep B 37.5 mcg__

Total = 187.5 mcg

-FDA Center for Biologics Evaluation and Research

Aluminum

Between 1999-2003, vaccine manufacturers reduced levels of mercury and increased aluminum (The Vaccine Book, Dr. Robert Sears)

According to the FDA a toxic dose for a baby is According to the FDA- a toxic dose for a baby is between 10 - 20 micrograms (mcg)

Immediately after birth, infants receive the Hep.B vaccine containing 250 mcg of aluminum

At 2 months age = 1,225 mcg

Aluminum Effects

Accumulates in various tissues in the body, including the kidneys, brain, lungs, liver and thyroid.

Competes with calcium for absorption and it can result in reduced skeletal mineralization. In infants this retards growth. Interferes with phosphorous absorption zinc and Interferes with phosphorous absorption, zinc and selenium.

Neurofibrillary degeneration Aluminium, neurofibrillary degeneration and Alzheimer's

disease. Brain.1976 Mar;99(1):67-80 Decreases cortical function Decreases glutathione

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Symptoms of Aluminum Toxicity

Muscle Aches Weakness Bone Pain Speech Problems Anemia

Digestive Problems Mental Confusion Headaches Memory Loss Nervousness Anemia

Premature Osteoporosis Impaired Immune

System Impaired Kidney

Function Impaired Iron Absorption

Nervousness Heartburn Emotional Instability Disturbed Sleep Impaired Intellect Loss of Coordination

Aluminum Exposure From Vaccines

A Child Fully Vaccinated at 18 months:

Hep B = 250 mcg X 2DTaP = 625 mcg X 4Hib = 225 mcg X 3PCV = 125 mcg X 4Hep A = 250 mcg X 2

+_____________________________

Dr. Bob Sears “America’s Pediatrician”

“This is disgraceful and dangerous. My instinct was to assume that the

issue had been properly researchedissue had been properly researched, and that the studies had been done on

healthy infants to determine their ability to rapidly excrete aluminum…

no studies have been done!”

American Academy of Pediatrics

“Aluminum is now being implicated as interfering with a variety of cellular and metabolic processes in the nervous system

and in other tissues.”

PEDIATRICS Vol. 97, 1996, pp. 413-416

Aluminum Studies

2005 – Autoimmunity

“Infection, vaccines and other environmental triggers of autoimmunity.”

2009 – Journal of Inorganic Biochemistry“Aluminum hydroxide injections lead to motor

deficits and motor neuron degeneration.”

Aluminum Studies

2011 – Journal of Inorganic Biochemistry

“ … the data indicates that the correlation between Al in vaccines and ASD may be causal.”

2011 – Current Medical Chemistry

“In particular, aluminum in adjuvant form carries a risk for autoimmunity, long-term brain inflammation and associated neurological complications. ”

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Vaccines & Autism

2009 - Annals of Epidemiology“U.S. male neonates vaccinated with hepatitis B

vaccine had a 3-fold greater risk of ASD”

2011- Journal of Toxicology and Environmental Health

“A Positive Association found between Autism Prevalence and Childhood Vaccination uptake across the U.S. Population”

AUTISM PREVENTION:

AVOIDAVOID ACETAMINOPHEN

Autism Prevention– Avoid Acetaminophen

Remember, all medications during pregnancy are potentially dangerous!

During pregnancy, mothers of autistic children commonly suffer more bacterial and viral infections and fevers, which could affect the fetus to predispose the child for autism.

These mothers often take acetaminophen to treat the infections. Acetaminophen overdose depletes the liver’s supplies of sulfate

and glutathione, impairing its ability to detoxify and excrete harmful substances. Therefore, the fetus could be impaired by the mothers consuming acetaminophen.

Children given acetaminophen after the MMR vaccine were significantly more likely to become autistic than children given ibuprofen.

Tylenol

Acetaminophen (Tylenol) toxicity is the foremost cause of acute liver failure in the Western world.

Accounts for most drug overdoses in the Accounts for most drug overdoses in the United States, the United Kingdom, Australia and New Zealand.

Tylenol, Asthma & Eczema

Use for fever in the first year of life was associated with an increase in the incidence of asthmatic symptoms at 6–7 years

Also associated with an increased incidence of rhinoconjunctivitis and eczemaj

Beasley, Richard; Clayton, Tadd; Crane, Julian; von Mutius, Erika; Lai, Christopher; Montefort, Stephen; Stewart, Alistair (2008). "Association between paracetamol use in infancy and childhood, and risk of asthma, rhino conjunctivitis, and eczema in children aged 6–7 years: analysis from Phase Three of the ISAAC programme". The Lancet 372

(9643): 1039–1048.

Tylenol + MMR & Autism

A study of 83 children with autism and 80 children without the condition showed that use of acetaminophen or Tylenol after measles-mumps-rubella vaccination was associated with much greater risk of autism.

Among children aged younger than 5 years, those h d l l f i i

g g y g 5 y ,who used Tylenol after MMR vaccination were 6 times as likely to develop autism as those who did not.

Acetaminophen (paracetamol) use, measles-mumps-rubella vaccination, and autistic disorder: the results of a parent survey. Autism. 2008

May;12(3):293-307

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Epidemic of Sick Children… How? Autism: Underlying Conditions

Heavy Metal Toxicity

Inability to Digest Proteins

GI Dysbiosis GI Dysbiosis

Food and Inhalant Allergies

Nutritional Deficiencies

GI and/or Brain Inflammation

REVERSING AUTISM:HEAVY METAL

TOXICITYTOXICITY

Lead

Average bone lead level of a deceased person today averages approximately 1000 times higher than that of deceased people who lived 400-500 years ago.

Lead disrupts Ca channels and keeps them open 5X longer than normalg

Alters mitochondria and neurotransmitters Sensory, motor, cognitive (↓ IQ) Behavior and learning disorders

Ericson, J.E.; Shirahata, H.; Patterson, C.C. (1975), "Skeletal concentrations of lead in ancient Peruvians", N. Engl. J. Med. 300: 949–51

Sources of Lead Exposure

Air pollution Ammunition Auto exhaust Batteries Containers for

Insecticides Foods (if grown in lead

contaminated soil) Lead based paint Lead-glazed pottery Containers for

corrosives Contaminated soil Cosmetics Fertilizers Hair dyes

Lead glazed pottery Pesticides Tobacco smoke Water (if transported

via lead pipes)

Symptoms of Lead Toxicity

Abdominal pain ADD Adrenal insufficiency Allergies, anemia Anxiety, arthritis Blindness

Gout Headaches Hostility Hyperactivity Hypertension Hypothyroidism

Nightmares Nausea Numbness Parkinson's disease, Psychosis Renal dysfunction

Cardiovascular disease Colic Constipation Convulsions Depression Dyslexia Epilepsy Fatigue

Impotence Liver dysfunction Mental retardation Mood swings Menstrual problems Muscular dystrophy Multiple sclerosis Nephritis,

Schizophrenia Seizures Stillbirths SIDS Tooth decay Vertigo Weight loss

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Aluminum Exposure

Vaccines

Cookware

Foil

Flatware

Cans Used for Food & Drink

Nasal Sprays

Baby Powder

Dentures

Lipsticks

Talcum Powder Cans Used for Food & Drink

Antacids

Buffered Aspirin

Vaginal Douches

Hemorrhoid Medications

Anti-Diarrhea Medications

Astringents

Talcum Powder

Baking Powder

Self-Rising Flour

Table Salt

Pickling Salt

Processed Cheese

Cake Mix

Symptoms of Aluminum Toxicity

Muscle Aches Weakness Bone Pain Speech Problems Anemia

Digestive Problems Mental Confusion Headaches Memory Loss Nervousness Anemia

Premature Osteoporosis Impaired Immune

System Impaired Kidney

Function Impaired Iron Absorption

Nervousness Heartburn Emotional Instability Disturbed Sleep Impaired Intellect Loss of Coordination

Arsenic, cadmium, copper, and antimony

Found in chicken, pipes, fire retardant bed clothes and mattresses…..everywhere

Damage CNS

F d d l d d ith d i Food dyes are loaded with cadmium

Toxic metal testing options

Hair analysis Shows past exposure and excretion Holmes and Adams found Hg levels 1/8 of normal kids "Mercury in first-cut baby hair of children with autism verses

typically-developing children," Toxocological & Environmental Chemistr (2008) 1 14Chemistry (2008), 1-14.

Reveals general mineral patterns Easy to obtain and inexpensive

Provocative Urine Test Administer a chelating agent such as DMSA and then

collect urine for 24 hours and measure output of metals.

Hair Elements- Sample Report

REVERSING AUTISM: FOOD ALLERGIES

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Food Allergies

High prevalence of food allergies seen in autism spectrum disorders 8% of children under the age of 3 Eight types of food account for over 90% of allergic reactions in

affected individuals: milk, eggs, peanuts, tree nuts, fish, shellfish, soy, and wheat

In 2007, the reported food allergy rate among all children younger th 18 18% hi h th i 1997than 18 years was 18% higher than in 1997.

29% of children with food allergy also had reported asthma Approximately 27% of children with food allergy had reported

eczema or skin allergy Over 30% of children with food allergy also had reported

respiratory allergy Children with food allergy are two to four times more likely to have

other related conditions such as asthma and other allergies.

http://www.cdc.gov/nchs/data/databriefs/db10.pdf

Delaying Solids Decreases Food Allergies

Prolonged exclusive breastfeeding results in a lower incidence of food allergies.

Until about s6 mos of age, babies possess what is often referred to as an "open gut." This means that the spaces between the cells of the small intestines will readily allow intact macromolecules, including whole proteins and pathogens to pass directly into the bloodstreampathogens, to pass directly into the bloodstream.

This allows beneficial antibodies in breastmilk to pass more directly into baby's bloodstream, but it also means that large proteins from other foods can pass too.

During baby's first 4-6 months, while the gut is still "open," antibodies (sIgA) from breastmilk coat baby's digestive tract and provide passive immunity, reducing the likelihood of illness and allergic reactions before gut closure occurs.

Allergy 1992 Oct;47(5):545-53.

IgG -antibodies produced when a germ attacksin a subsequent invasion and may also beinvolved in causing food allergies.

IgA- antibody that is involved with protection ofthe lining of the nasal passages and intestinal

Types of Antibodies

g p glining from germs.

IgM-usually the first antibody produced by theimmune system when a new germ isencountered and is the body’s early defensesystem.

IgE-the antibody most widely known for itsinvolvement in allergies of all kinds

IgE Versus IgG Allergic Reaction

IgE Releases histamine

Tested by skin pricks

h l i h k

IgG Does not release histamine

No skin prick reaction

h l i h k Anaphylactic shock

Symptoms immediate

Most common allergy test

Limited clinical usefulness

No anaphylactic shock

Symptoms may be delayed

Uncommon allergy test

Extreme clinical usefulness

Most Common IgG Food Allergies

Gluten (wheat, rye, barley)

Casein (milk, milk

Peanuts

Chocolate

Egg

Baking Yeast( ,products)

Soy

Citrus (orange, lemon, grapefruit)

Baking Yeast

Brewer’s yeast (Saccharomyces cerevisiae)

Sugar

Symptoms of Food Allergy

Aberrant behavior

Hyperactivity

Fibromyalgia

Irritable Bowel Syndrome Irritable Bowel Syndrome

Chronic Fatigue

Epilepsy

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Milk Allergy and Behavior

Chronic juvenile defenders drink much more milk than controls.

In a trial, 70% of juveniles in a detention center tested positive for a milk allergy.

Milk Allergy and Behavior

In juvenile prison in USA, the incidence of unacceptable behavior and violence dropped greatly after substituting OJ for milk.for milk.

When milk returned, unacceptable and violent behavior returned.

Schauss AG. Critical analysis of the diets of chronic juvenile offenders.

J. Orthomol Psych.8:149-157,1979.

Food Allergy & Fibromyalgia

After 2 weeks without eating any of the potential food allergens, nearly half of the patients reported "significant reduction of pain," and 76% reported a reduction in other symptoms such as headache, fatigue, bloating, heartburn, and breathing difficulties.

The most common problem-causing foods or ingredients for the patients in this study were corn, wheat, dairy, citrus and sugar.

Elimination of food allergies reduces symptoms in FMS Annual Meeting of the American College of Nutrition in Orlando,

Florida October 2001

Food Allergy & IBS

Recent evidence suggests significant reduction in IBS symptom severity in patients on elimination diets, provided that dietary elimination is based on foods against which the individual had raised IgG antibodies.

These findings should encourage studies dissecting the mechanisms responsible for IgG production against dietary antigens and their putative role in IBS.

Food allergy in irritable bowel syndrome: new facts and old fallacies. Isolauri E et al Gut. 2004 Oct;53(10):1391-3.

Milk Consumption & Parkinson’s

The 30-year study followed over 7,500 men between the ages of 45 and 68 who were part of the Honolulu Heart Program.

During the study, 128 men developed Parkinson's disease, which is a degenerative gdisease of the nervous system.

Specifically, those who drank more than 16 ounces of milk each day were 2.3 times more likely to develop Parkinson's than those who didn't drink any milk.

Parkinson’s and milk consumptionNeurology March 22, 2005;64(6):1047-1051

63 children with epilepsy 45 children had epilepsy with migraine, hyperkinetic behavior, or

both. Of the 45 children who had epilepsy with recurrent headaches,

abdominal symptoms, or hyperkinetic behavior, 25 ceased to have seizures and 11 had fewer seizures during diet therapy

Headaches abdominal pains and hyperkinetic behavior ceased in

Food Allergy & Epilepsy

Headaches, abdominal pains, and hyperkinetic behavior ceased in all those whose seizures ceased, and in some of those whose seizures did not cease

Symptoms recurred with 42 foods, and seizures recurred with 31; Most children reacted to several foods

Of 24 children with generalized epilepsy, 18 recovered or improved (including 4 of 7 with myoclonic seizures and all with petit mal)

Egger J,et al J Pediatr 1989 Jan;114(1):51-8Oligoantigenic diet treatment of children with epilepsy and migraine.

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Food Allergy & Epilepsy

In double-blind, placebo-controlled provocation studies, symptoms recurred after foods in 15 of 16 children, including seizures in eight N t d h l b No symptoms recurred when placebo was given.

Egger J,et al J Pediatr 1989 Jan;114(1):51-8Oligoantigenic diet treatment of children with epilepsy and

migraine.

Food Allergy & ADHD

Food allergies in 17 children diagnosed with ADHD who ranged in age from 7 to 10 years old.

56% with ADHD had positive food allergy tests

6% to 8% positive food allergy tests among children in the general population.

Dr. Bellanti et al.International Center for Interdisciplinary Studies of Immunology at Georgetown Univ Med Ctr, Washington,DC.

59th annual meeting of the Amer College of Allergy, Asthma, and Immunology Dec 2001

Abnormal Peptides, Autism & Schizophrenia Abnormal peptides in both patients with autism and

schizophrenia High titers of IgG antibodies to gliadin (wheat)

found in 87% of patients with autism and 86% of patients with schizophrenia

High titers of IgG antibodies to bovine casein found g gin 90% of patients with autism and 93% of patients with schizophrenia

A gluten and casein free diet caused significant improvement in 81% of patients with autism within 3 months.

R Cade et al. Autism and schizophrenia: Intestinal disorders. Nutritional Neuroscience 3: 57-72,2000

University of Florida, USA

Comprehensive Food Allergy IgGIgG

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Addressing Food Allergies

Strict elimination of high allergens for 6 months.

Avoid moderate allergens – consume no more than once per week.p

Caution with consumption of low allergens –no more than twice a week on a rotation basis.

Retest after 6 months

REVERSING AUTISM: GLUTEN & CASEIN

INTOLERANCEINTOLERANCE

Digestion of Peptides

Proteins Peptides Amino Acids

In autism, high occurrence of inability to break down wheat (gluten) and milk (casein). (Lightdale et al 2001)

For proteins gluten and casein, the intermediate peptide created during digestion are opioid peptides called exorphins.

Endorphins are group of peptide hormones made by the body that bind to opiate receptors and are found mainly in the brain. Endorphins are similar in structure and effect to opiate drugs. Endorphins reduce the sensation of pain and affect emotions.

If exorphin peptides get absorbed via leaky gut -> affects neurons and immune system

Exorphin Peptides

These peptides bind to opiate receptors in the brain, mimicking the effects of opiate drugs, like heroin and morphine.

Cade et al found undigested exorphin peptides in urine of ASD kidsurine of ASD kids

DPP4 (dipeptidyl peptidase IV) enzymes are responsible for degrading “exorphins” like casomorphin from casein and gluteomorphin from gluten

DPP4 can inactivated by toxins such as lead, cadmium and organophosphates

Puschel et al., European J. Biochem, vol 126, p. 359-365 (1982)

Casomorphin & Gluteomorphin

Clouded mental function Insomnia Diarrhea Impaired Social Connectionp Blocking of pain messages Dilated pupils Crave opioid effect

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Affect on Brain

Casomorphin and Gluteomorphin affect the

TEMPORAL LOBES O O Sof the brain,

which are involved in

speech and auditory integration

TACA

Advocates the Gluten-Free/ Casein –Free Diet (GFCF)

“The GFCF diet helps 85% of the children out there in improving everything about theirthere in improving everything about their learning disabilities and overall well being. This includes speech, better bowel movements than before, better sleep patterns, less cranky behaviors, less stimmy behaviors, less in a fog/daze look and more ready to learn than ever before.”

Urinary Peptides Test

Caution – can show false negatives!

If either casomorphin or gliadorphin are elevated, eliminate both

Administer enzymes for gluten and casein

REVERSING AUTISM: NUTRITIONAL & BIOCHEMICAL

DEFICIENCIESDEFICIENCIES

Nutritional Deficiency

Yeast

Parasites

Selective diet

Destroyed gut Destroyed gut

Lack of enzymes

Disrupted metabolic pathways

Common Deficiencies in Chronic Illness

Vitamins – B6, MB12, A, C, D

Minerals – Calcium, Magnesium, Zinc

Antioxidants – Glutathione

Essential Fatty Acids Essential Fatty Acids

Cholesterol

Beneficial Bacteria

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Zinc deficiency looks like…

Acne

Psoriasis

White spots on nailsp

Canker sores

Sparse hair

Nail Changes

Zinc Spots Rigid Discolored Nails

Keratosis pilaris

Appearance of rough bumps on the skin, referred to as chicken skin.

Most often appears on the back and outer sides of theback and outer sides of the upper arms (though the lower arms can also be affected), and can also occur on the thighs and tops of legs, flanks, buttocks, or any body part.

Essential fatty acid deficiency

Methyl B12

Out of the B12 family, only methyl-B12 has the ability to activate the pathway that is responsible for the body’s entire sulfur-based detoxification system.

It is this pathway that is responsible for the formation It is this pathway that is responsible for the formation of homocysteine, the “crossroads” molecule that is responsible to create cysteine, taurine, and glutathione.

Unfortunately, many children have a defect in the enzyme that converts B12 to Methyl B12 for the body.

How effective is supplementing methyl B12?

Some doctors claim that up to 94% of children have been found to respond to methyl-B12 therapy.

What changes are seen?E i f i i i d i % f hild hi lik Executive function is improved in 90% of children – things like awareness, cognition, appropriateness, eye contact when called, and “just being more like a normal kid.”

Speech and language is improved in 80% of children

Socialization and emotion is improved in 70% of the children –initiation and interactive play, understanding and feeling emotions, possibly for the first time or to a much more normal degree, etc.

Deficient Glutathione

Glutathione is one of the most powerful antioxidant in the body.

Responsible for detoxification: binding and Responsible for detoxification: binding and removal of mercury, lead, cadmium, arsenic, nickel, tin, antimony, and many other lesser-known heavy metals that also bind to glutathione’s sulfur group.

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Deficient Glutathione & Autism

Most children with ASD are significantly deficient in glutathione and this deficiency may significantly contribute to the symptoms associated with autism.

It is believed by several autism researchers that It is believed by several autism researchers that mercury poisoning disrupts the transsulfurrationmetabolic pathway, reducing the body's natural production of glutathione.

James SJ, Rose S, Melnyk S, Jernigan S, Blossom S, Pavliv O, Gaylor DW. Cellular and mitochondrial glutathione redox imbalance in lymphoblastoid cells derived

from children with autism. FASEB J. 2009 Mar 23.

Deficient Glutathione & Autism

Results of glutathione deficiency include: Brain damage Impaired ability of neurons to communicate with each other Impaired proliferation of nerve cells during early childhood

development Die-off of neurons within the brain.

Glutathione deficiency maybe indicated by: Coordination problems Mental disorder Tremors Twitching Nervous system disorder Difficulty in balancing.

Cholesterol

Cholesterol plays an important role in many physiological factors in the body.

The formation of sterol hormones, cell membranes, and bile acids, would not be possible without cholesterol.

The brain is the most cholesterol rich organ in the body g yrequiring a large amount to sustain the myelin sheath which coats nerve cells and helps conduct electrical impulses.

Cholesterol is essential for the proper functioning of serotonin receptors which are responsible for good mood and oxytocin receptors needed for good social interactions and interpersonal bonding.

Deficient Cholesterol

Risks associated with insufficient cholesterol values of 160 mg/dL or less:

•Violent behavior•Suicide

•Hyperthyroidism•Liver disease

Modai I, et al. Serum cholesterol levels and suicidal tendencies in psychiatric inpatients. J Clin Psychiatry. 1994 Jun;55(6):252-4.

•Depression•Anxiety•Delinquency•Bipolar disease•Parkinson’s disease•Celiac disease

•Malabsorption, and malnutrition •Lack of attention•Increased infections•Motor difficulty

Cholesterol & Cognition

Adults with low, “desirable” cholesterol levels (less than 200 mg/dL) performed poorer on a range of cognitive functions than participants with borderline-high cholesterol levels (200–239 mg/dL) or high cholesterol levels (more than 240 mg/dL).

The authors concluded that lower cholesterol levels in The authors concluded that lower cholesterol levels in adults are associated with poorer performance on cognitive measures, which place high demands on abstract reasoning, attention/concentration, word fluency, and executive functioning.

Elias P, et al. Serum cholesterol and cognitive performance in the Framingham Heart Study. Psychosomatic Medicine 67: 24-30

(2005).

Cholesterol, CFS & Dyslexia

Lower cholesterol values were also found in chronic fatigue syndrome and in children with dyslexia. [1,2]

More recently, it has been discovered that cholesterol is needed to activate a protein called S i H d h (SHH) hi h l t l lSonic Hedgehog (SHH) which plays a central role in neural patterning, mood regulation, and cerebral development.

[1]Talking Point, Official Journal of the M.E./C.F.S. Society (SA) Inc. June 2001, page 38.

[2] Saviour P, et al. Familial patterns and biological markers of dyslexia. Int J Hum Genet, 9(1): 21-29 (2009)

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Deficient Cholesterol & Autism

John Hopkins University – 19% of children with autism have significantly low levels.

Research duplicated by Great Plains Lab

Cholesterol Supplementation

Research has shown that supplementation with cholesterol quickly reversed the symptoms in individuals with deficient cholesterol:

Sleeping through the night Overcoming aberrant behaviors Learning to walk Speaking for the first time Increased social responsiveness Increased social responsiveness Decreased rate of infections Reduced skin rashes Marked reduction in self-hurtful behaviors Improved muscle tone Decreased tactile defensiveness Improved behavior overall

Sikora DM, Pettit-Kekel K, Penfield J, Merkens LS, Steiner RD. The near universal presence of autism spectrum disorders in children with Smith-Lemli-Opitz

syndrome. Am J Med Genet A. 2006 Jul 15;140(14):1511-8.

Eye Poking

Adult patient with hypocalcinuria removed one of his eyes and attempted to injure the other. Thirty-six hours after being placed on calcium supplements he stopped touching his good eyesupplements he stopped touching his good eye. No further ocular self-injury observed.

Over twenty documented cases of people suffering with autism poking their eyes out.

“Clinical presentations of patients with autism and hypocalcinuria” Mary Coleman, Developmental Brain

Dysfunction, 7, 1994, pp.63-70.

Self-Injury – a response to extraordinary pain

Child with no ability to communicate.

After addressing GI issues, self-injury ceased.

Why Self-injury?

Release of beta-endorphins can dampen pain… Method of 'gating' the painMethod of gating the pain.

Self-injury may reduce or dampen the pain located in another area of the body.

Nutrient Deficiency Associated with AutismCONCLUSIONS: Oral vitamin/mineral supplementation is

beneficial in improving the nutritional and metabolic status of children with autism, including improvements in methylation,

l t thi id ti t lf ti ATPglutathione, oxidative stress, sulfation, ATP, NADH, and NADPH. The supplement group had significantly greater improvements than did the placebo group on the PGI-R Average Change. This suggests that a vitamin/mineral supplement is a reasonable adjunct therapy to consider for most children and adults with autism.

Effect of a vitamin/mineral supplement on children and adults with autism. BMC Pediatr. 2011 Dec 12;11:111.

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Vitamin D

Produced when our skin is exposed to sunlight

Cholesterol is needed for vitamin D synthesis

Every cell in the brain has vitamin D receptors. The receptors control gene expression or how p g pgenetic material is used.

Infection during pregnancy could increase the risk of autism. Influenza during pregnancy raises the maternal body temperature. This can have adverse effects on the developing fetus. High levels of vitamin D may lower the risk for influenza.

Vitamin D & Autism Research

CONCLUSION: Vitamin D deficiency--either during pregnancy or

early childhood--may be an environmental trigger for ASD in individuals genetically predisposed for the broad phenotype of autism. On the basis of the

lt f th t i f thresults of the present review, we argue for the recognition of this possibly important role of vitamin D in ASD, and for urgent research in the field.

Lapillonne, A, Vitamin D deficiency during pregnancy may impair maternal, fetal outcome. Med Hypotheses 2010 Jan:74(1) 71-5 Epub

1009 Aug 08

Vitamin D & Autism

Vitamin D has remarkable antioxidant, anti-inflammatory, and anti-autoimmune properties. In vitro, in vivo, and animal experiments provide compelling data for vitamin D's role brain proliferation, differentiation, neurotrophism, neuroprotection, neurotransmission, and p , ,neuroplasticity. It also upregulates glutathione, upregulates a suit of genes involved in DNA repair and raises the seizure threshold. Adequate, perhaps pharmacological, doses of vitamin D may have a treatment effect in the core symptoms of autism.

Autism, will vitamin D treat core symptoms? Med Hypotheses. 2013 May 29

Vitamin D & Autism

One of the hallmarks of autism is oxidative stress. This leads to early cell death. There are many papers in the literature reporting that vitamin D reduces oxidative stress.

Patient Strategy – Vitamin D

Optimal levels 80-100 ng/mL

Ensure that during pregnancy, maternal levels are adequate.

Ensure proper sun exposure for both mother Ensure proper sun exposure for both mother and child.

Supplement with vitamin D3 if needed

REVERSING AUTISM: GI DYSBIOSISGI DYSBIOSIS

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“All disease begins in the gut.”

– Hippocrates

Leo Kanner - 1943

Feeding IssuesImmune IssuesGastrointestinal Issues

GI Problems in Autism

A significant percentage of children with autism spectrum disorder have gastrointestinal symptoms including diarrhea, constipation, abdominal pain, reflux, gaseousness and foul smelling stools.

Andrew Wakefield

Lancet 1998 – paper written by Wakefield and 12 other doctors about 12 children with autism.

Possible link between bowel disease, autism, and the MMR vaccine. Eight of the twelve children had the onset of behavioral problems within two weeks of MMR.

"We identified associated gastrointestinal disease and developmental regression in a

group of previously normal children, which was generally associated in time with

possible environmental triggers. These possible triggers were reported to be MMR in

eight cases, and measles infection in one.”

VERY CONTROVERSIAL

Dr. Timothy Buie

Pediatric gastroenterologist from Harvard University and Mass. General Hospital is perhaps the nation’s leading authority on thisperhaps the nation s leading authority on this condition in autism.

Performed 400+ gastrointestinal endoscopies along with biopsies and digestive enzyme function tests in children with ASD in the nation's largest study on autism and GI problems.

Consistent Findings

Biopsy - chronic inflammation in the digestive tract, along with esophagitis, gastritis and enterocolitis.

Enzyme testing showed that the children have enzyme levels below normal.

These disorders, especially reflux esophagitis and disaccharide malabsorption may contribute to the behavioral problems of non-verbal autistic children.

“These children are ill, in distress and pain and not just mentally and neurologically

dysfunctional.”- Dr. Buie

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In the beginning…

GI tract of a normal fetus is STERILE.

During birth and rapidly thereafter maternal During birth and rapidly thereafter, maternal bacteria and the surrounding environment colonize the infant's gut.

Immediately after vaginal delivery, babies have bacterial strains in the upper GI tract from the mothers’ feces.

In the beginning…

Vaginally born infants: take up to 1 month for their intestinal flora to be well established.

C ti i iti l i t lik l f C-section: initial exposure is most likely from environmental microbes such as the air, other infants, and the nursing staff.

C-section: gut flora may be disturbed for up to 6 months

After birth

Bacteria are transferred from the mother to the infant through suckling, kissing, and caressing.

All infants are initially colonized by large numbers of E. coli and t t i Withi f d b t i l b h 8 tstreptococci. Within a few days, bacterial numbers reach 108 to 1010

per gram of feces.

Breast-fed babies become dominated by bifidobacteria. (bifidobacterial growth factors in breast milk).

Formula-fed infants have high numbers of Enterobacteriaceae, enterococci, bifidobacteria, Bacteroides, and clostridia.

Microorganisms in the GI Tract

Bacteria (aerobic and anaerobic)

Yeast and Fungi (Candida)

Occasionally Protozoa Occasionally Protozoa

500 + different species of bacteria

30 – 40 species predominate, keeping a healthy balance

To Compare…

The average human body has about ten trillion cells

There are an estimated ten times that number of microorganisms in the intestines.

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Essential functions

Train the immune system to only respond to pathogens

Aid in digestion Produce vitamins for the host (ex- biotin and

vitamin K)P d h t di t th h t t t f t Produce hormones to direct the host to store fats

Detoxification Protection against some diseases Fats, TG, Cholesterol regulation Balance Th1 and Th2 arms of immune system Help the body absorb nutrients such as calcium,

magnesium, and iron.

The Gut and Immunity

Flora & Immunity

Bacteria stimulate lymphoid tissue in the gut mucosa to produce antibodies to pathogens.

The immune system recognizes and fights harmful bacteria, but leaves the helpful species alone, a tolerance developed in infancy.developed in infancy.

The first bacteria to settle in are able to affect the immune response, making it more favorable to their own survival and unfavorable for competing species.

The first bacteria to colonize the gut are important in determining the person's lifelong gut flora makeup.

Maintain Balance Between TH1 & TH2

TH1 arm of immune system – appropriate reaction to the environment. The immune system should not overreact to harmless things, such as pollen and benign foodssuch as pollen and benign foods.

TH2 arm of the immune system – allergic reactions

Preventing Allergy

Infants and children with allergies have different gut flora from those without allergies.

Higher amounts of harmful species C difficileg p ffand S aureus and lower prevalence of Bacteroides and Bifidobacteria.

Helpful gut flora stimulate the immune system and "train" it to respond properly to antigens.

A lack of these bacteria in early life leads to an inadequately trained immune system which overreacts to antigens.

Vitamin Production

B vitamins have a very short half-life in the body.

Even with a B vitamin-rich meal or supplements, will be depleted within a few hours.will be depleted within a few hours.

Nature’s answer – beneficial flora in gut provide a constant stream of B vitamins

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Detoxification

Beneficial bacteria are able to:

Neutralize toxic substances

Chelate heavy metals and other toxins

Short Chain Fatty Acids

Increase the gut's absorption of water

Reduce counts of damaging bacteria

Increase and control growth of human gut cells

They may also cause lymphoid tissue near the gut to grow.

Help the body absorb nutrients such as calcium, magnesium, and iron.

Flora & Obesity

Obese mice have a distinct gut flora compared to (normal) lean mice- change in the ratio between bacteria species.

In humans- shift in the ratio between bacterial divisions In humans- shift in the ratio between bacterial divisions - Firmicutes and Bacteroidetes.

The difference is the energy-reabsorbing potentialof different bacteria, especially in the digestion of fatty acids and dietary polysaccharides.

Research: gut flora of obese mice was transplanted into germ free recipient mice lead to an increase in weight despite a decrease in food consumption.

Journal of Infection and Immunity

When oral antibiotics are administered, the total population of bacteria, including beneficial,

d d b f t f 1 000was reduced by a factor of 1,000.

As good bacteria is killed off, potentially harmful bacteria increase rapidly.

Berg R. Promotion of enteric bacteria from the gastrointestinal tracts of mice by oral treatment with penicillin clindamycin, or metronidazole. Infection

and Immunity 33:854-61, 1981.

ANTIBIOTICS KILL… What is an ANTIBIOTIC?

Waste product from a living fungal colony.

These chemicals, Mycotoxins, spread and kill anything that might compete with the fungus for food.

-Doug Kaufmann

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Overuse of Antibiotics in the USA

The average child gets 10 to 20 courses of antibiotics by age 18.

A 2003 study published in the Journal of the American Medical Association showed that doctors prescribed antibiotics for more than 60%doctors prescribed antibiotics for more than 60% of adults with upper respiratory tract infections, which are usually caused by viruses.

A 2010 study published in Infection Control and Hospital Epidemiology found that doctors sometimes kept patients on antibiotics even after tests showed they had viral infections.

Antibiotic Misuse

Antibiotics have no effect on viral infections such as the common cold. They are also ineffective against sore throats, which are usually viral and self-resolving.[1]

Most cases of bronchitis (90–95%) are viral as well, passing after a few weeks—the use of antibiotics suchpassing after a few weeks the use of antibiotics such as ofloxacin against bronchitis is superfluous and can put the patient at risk of suffering adverse reactions.[2]

[1]Little P, Gould C, Williamson I, Warner G, Gantley M, Kinmonth AL (August 1997). "Reattendance and complications in a randomised trial of

prescribing strategies for sore throat: the medicalising effect of prescribing antibiotics“. BMJ 315 (7104): 350–2.

[2]Hueston WJ (March 1997). "Antibiotics: neither cost effective nor 'cough' effective". The Journal of Family Practice 44 (3): 261–5.

Antibiotics Should Not be Used For:

Common Viral or Fungal Infections:

Ear infection Strep throat Bee Stings

C ld Common cold Urinary tract infection Bronchitis Flu (influenza) Most coughs Most sore throats Stomach flu (viral gastroenteritis) Preventive medicine during or after birth in hospital

Antibiotic Misuse

A 2003 study concerning the proper use of antibiotics in the emergency room revealed that 99% of instances contained some sort of error.

Out of the 100 patients studied, 81 inappropriately received a prescription for fluoroquinolone. 43 did not call for fluoroquinolone as a first-line agent 27 presented no evidence of bacterial infection 27 presented no evidence of bacterial infection 11 had questionable need for such therapy. Of the 19 cases that both called for and received fluoroquinolone, only

one received both the correct dose and duration of therapy.

Lautenbach E, Larosa LA, Kasbekar N, Peng HP, Maniglia RJ, Fishman NO (March 2003). "Fluoroquinolone utilization in the emergency departments of academic

medical centers: prevalence of, and risk factors for, inappropriate use". Archives of Internal Medicine 163 (5): 601–5. doi:10.1001/archinte.163.5.601.

PMID 12622607.

Antibiotic Risks

Antibiotics can cause severe reactions

In the US, this accounts for billions of dollars of expense within the healthcare system.

Adverse effects from antibiotics account for Adverse effects from antibiotics account for nearly 25% of all adverse drug reactions amongst hospitalized patients.

Beringer PM, Wong-Beringer A, Rho JP (January 1998).

"Economic aspects of antibacterial adverse effects". PharmacoEconomics 13 (1 Pt 1): 35–49.

Dysbiosis

AKA dysbacteriosis

The condition of having i bi l i b lmicrobial imbalances on or within the body.

Dysbiosis is most prominent in the

digestive tract or on the skin.

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Intestinal Imbalance

Yeast and bacteria produce chemical byproducts that are usually present in small quantities.

An overgrowth of these microorganisms results An overgrowth of these microorganisms results in much higher concentrations of these toxins.

Absorbed from intestine Blood Body

Symptoms of Clostridia Overgrowth

Irritability Agitated Aggression Biting, Kicking, Screamingg, g, g Head Banging Decreased Eye Contact Loose Stools

Group A beta-hemolytic Streptococcus

Associated with PANDAS (Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcal Infections)

AutismT d Tourette syndrome

Tic disorder Parkinson’s disease OCD

Killing GI Bacteria

Results in proliferation of YEAST (Candida)

1. Yeast have no competition for food as it passes through system after mealspasses through system after meals.

2. Yeast may actually be stimulated by antibiotics and heavy metals.

Animal Trials

Infants much more susceptible than older animals. Once exposed to Candida at an early age developed persistent candidiasis.

When young animals are given antibiotics at an early age, the Candida in intestines

increased 130 times

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Why Does This Imbalance Persist?

During the first 2 years of life, the baseline environment for immune development is established.

The immune system takes “inventory” of all the cells The immune system takes inventory of all the cells present during fetal development and after birth. Also applies to bacteria and yeast in the GI tract.

Cells on this “inventory” are given immune tolerance and will not be attacked later by immune system.

IMMUNILOGICAL MEMORY

Effects of Yeast Overgrowth

Systemic Toxicity Digest carbs & sugars via alcoholic fermentation. Major products of metabolism are alcohol

(ethanol) and acetaldehyde (this is the chemical responsible for the main symptoms of a hangover) p y p g ) Disastrous affects as it circulates the body. Uses up enzymes needed to process vitamin B6 Reduces RBC ability to carry oxygen Reacts with neurotransmitters to create opiates Affects nerve cell communication in the brain. Linked to autoimmune disease Targets myelin and connective tissue

Yeast-induced Nutritional Deficiency

Nutritional deficiency, regardless of diet Arabinose can block coenzymatic reactions causing vitamins to not be utilized. Acetylaldehyde interferes with B vitamin processing.

Leaky Gut Attach themselves to the intestinal wall with digestive

enzymes.enzymes.

Intestinal cells may die, lining appears like Swiss cheese under microscope.

Pediatr Res. 2002 Mar;51(3):290-5. Cecal colonization and systemic spread of Candida albicans in mice treated with antibiotics and dexamethasone.

Bendel CM, Wiesner SM, Garni RM, Cebelinski E, Wells CL. Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Yeast Destruction to GI Wall

This makes the intestine more permeable (Leaky Gut Syndrome).

This causes an even higher absorption rate of the toxic alcohol and acetaldehyde and other ybyproducts.

Candida themselves may actually be able to slip through and gain access to the rest of the body causing chronic immune reactions like allergies and autoimmune diseases.

Yeast & Autoimmunity

Autoimmunity & other immune problems Produce gliotoxins which are toxic to the immune

system Cause production of antibodies that react against

various parts of the human body (Brain Kidneyvarious parts of the human body. (Brain, Kidney, Pancreas, Spleen, Thymus, and Liver)

Oxalates Crystal deposition can occur in virtually all tissues of body,

including brain potentially severe symptoms and even lesions.

Yeast & Allergy

Recent research shows that antibiotics and the resulting increased Candida colonization of the intestinal tract leads to an increase in airborne allergies.

Mice given antibiotics had increased intestinal Candida colonization, which has been shown many times before, but

l h d llalso showed a concurrent increase in allergic responses to an airborne mold spore called Aspergillus fumigatus.

Infect Immun. 2004 Sep;72(9):4996-5003. Role of antibiotics and fungal microbiota in driving pulmonary allergic responses. Noverr MC, Noggle RM, Toews GB, Huffnagle GB.

Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109-0642, USA.

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Oxalates Play a Role In

Arthritis Joint Pain Interstitial cystitis Vegetarianismg Osteoporosis Heart Disease Heavy Metal Toxicity Thyroid Disease

Clues in history

Difficulty breastfeeding

Colic Gastro esophageal

Failure to thrive Frequent infections Frequent

antibiotics Gastro-esophageal reflux

Infantile eczema

antibiotics Poor sleep Food sensitivities

Signs & Symptoms of GI Dysfunction

Distended Abdomen

Abnormal posturing

Self-injurious

Complaints

Behavior changes prior to having a Self injurious

behavior

Unexplained Tantrums

Waking at Night

Irritability

p gbowel movement.

Hyperactivity

Crying

What does GI disease LOOK like in autism?

Symptoms of Yeast Overgrowth (Autism)

Stimming Toe-Walking Decreased Eye Contact Not engagedo g g d Unexplained silliness Echolalia Intensified sugar and carb cravings Irritability Loose stools or constipation

Healing Dysbiosis

Process that takes time.

Takes consistency and persistence.

Dysbiotic microorganisms have self-protection Dysbiotic microorganisms have self protection mechanisms for survival.

BIOFILM

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Healthy Biofilm

Embodies gastrointestinal barrier function. Allows for resistance against pathogenic

organisms. Interface for GI and systemic immune function

modulation Site for gut detoxification. Source of calories and nutrients

Biofilm Benefits for the Microbe

Increases survival, offers significant protection. Reduces shear force in colon from peristalsis. Protection from predators – amoebas,

bacteriophages etcbacteriophages, etc… Protection from body’s humeral and cell-

mediated immune responses. Between 10 and 1000 times more resistant to

antibiotics than planktonic species. Mature biofilm - Heterogeneous mixture of

microorganisms, exopolysaccharides, water, metals and debris.

Pathogenic Biofilm

Causes an inflammatory response to normal commensalmicroorganisms.

Compromises hosts defense against pathogens.

More than 80% of all microbial infections have developed More than 80% of all microbial infections have developed biofilms beginning in as little as two weeks from the onset of infection

Biofilm bacteria can resist up to 5000 times the antibiotic concentration that would normally be needed to resolve infections.

Recognized as major factor in chronic, persistent disease.

Biofilm Associated Infections

Chronic SinusitisBiofilms of both yeast and/or bacteria are found in 80% of patients undergoing sinus surgery.

Pulmonary InfectionsBacterial and fungal biofilms can cause chronic infection such as pneumonia cystic

Staph aureus biofilmon a catheter

chronic infection such as pneumonia, cystic fibrosis, and tuberculosis.

Kidney StonesStones consist of a complex composed of bacterial biofilm, and mineralized stone material.

Heart DiseaseInflammation of the smooth membranes which line the inside of the heart is caused by a complex biofilm composed of both bacterial LPS and host components. Biofilmshave been found on stents and other cardiac prosthetics.

Pseudomonas A. biofilm inside the alveoli in the lung

MRSA

Inner Ear Infections The majority of ear infections, (OM) are caused by biofilm bacteria and or fungal infection. OM is the most common illness for which children visit a physician, receive antibiotics, or undergo surgery in the United States. Two thirds of post operative tissue cultures have biofilms. Otitis Media Biofilm

Biofilm Associated Infections

Periodontal DiseaseOver 5000+ microbial biofilm species colonize the human mouth, causing tooth decay and chronic gum disease, and these bacteria can migrate throughout the body causing a variety of infections.

In many cases of periodontal infection C. albicansand S. aureus co-colonization makes it difficult to treat.

Chronic Wounds Biofilms have been implicated in chronic wounds, including antibiotic resistant Staph such as MRSA.

Dental Biofilm

MRSA

Autism, MS, Lyme

Chronic Neuro-Excitotoxicity

50-70% of MS patients test positive for infection. 80-100% of autistic children have abnormal yeast and bacterial loads.Biofilm Infections Found Include:

Resting Microglia

YeastBacteriaVirusesParasitesLyme Disease and co-infectionsMycoplasma

Brain and nerve cell loss, as seen in Autism, MS, and Parkinson’s

Chronic infections need to be treated to stop persistent activation of microglia and nervous system damage.

.

Activated Microglia

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Testing for Dysbiosis

Organic Acid Test (urine) 65 specific metabolic markers Yeast & Clostridia Oxalates

Comprehensive Stool Analysisp y Gut Flora Parasites Culture and sensitivity of gut bugs Digestion Absorption Immune status (SIgA) Inflammatory markers

Treating Yeast

Starve yeast with an anti-fungal diet Low carbohydrate

No sugar

Kill yeast with antifungal substances

Replenish body with commensal microbes Probiotics

Fermented foods / beverages

5 Methods of Biofilm Control: Utilizing Plant Based Medicines

• Inhibition of Quorum Sensing: determines gene expression, virulence, resistance, and the development of biofilms.

• Inhibition of Initial Attachment Phase of Biofilm Colonies

• Inhibition of Swarming Motility of Pathogenic Organisms• Inhibition of Swarming Motility of Pathogenic Organisms

• Efflux Pump / Multi Drug Resistance Pump Inhibitors

• Bacteriostatics / Bactericidals

Inhibition of Quorum Sensing

Botanicals which are known to inhibit Quorum Sensing:

- Garlic- Oregano- Bilberry- Bladderwrack

Prevention Adhesion /Disrupting BiofilmComponents

Essential Oils Oregano, Tea Tree, Galbanum, and Lavender are

antimicrobial essential oils used in the Biocidin® formula Phenolics such as Thymol interact with surface proteins of

bacteria, leading to an alteration of the cell surface and interrupt the initial attachment phase of biofilm formationinterrupt the initial attachment phase of biofilm formation.

Essential Oils may prove to be most useful in treating multi drug resistant gram negative bacteria such as Pseudomonas, Escherichia and Acinetobacter.

Inhibition of Swarming Motility of Pathogenic Organisms

Condensed Tannins Grape Seed and Bilberry contain condensed tannins that prevent

adherence of biofilms, and may inhibit swarming motility of bacteria.

OPC’s are also potentiators for Vit C, providing numerous benefits

Representative images of P. aeruginosa swarming motility under control conditions (A) and in the

presence of Cranberry (B) and Pomeranate (C) (both 100 μ/ml).

p , p g bin cellular health and anti-aging medicine.

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Efflux Pump Inhibitors:Inhibition of Multi Drug Resistance Pumps

Biocidin® and Olivirex® formulas contain tannins, berberine, and certain phenolics which have useful effects as efflux pump inhibitors.

Research indicates that these botanicals can potentiate conventional antibiotics and increase their effectiveness against a variety of both gram positive and gram negative organisms.

Goldenseal, Black Walnut, White Willow, Raspberry Leaf Bladderwrack, Uva Ursi, and Garlic are a few botanicals that have been studied as Efflux Pump Inhibitors.

Bacteriostatic and Bactericidal Botanicals

Bacterostatics: Prevent Replication of Bacteria.

Berberine containing plants such as Goldenseal and Gentian have demonstrated powerful bacteriostaticaction.

One study showed the effectiveness of berberineagainst Staph. epidermidis, one of the most g p p ,common causes of skin infections. Sub-minimal inhibitory concentrations block the formation of S. epidermidis biofilm.

Bactericidals: Terminate Bacteria

Olive Leaf , Goldenseal, Bilberry, Grape Seed, Shiitake, Noni, Black Walnut, Garlic, Gentian and Fumitory are potent bactericides. Oregano, Tea Tree , Lavender and Galbanum oils are essential oils with excellent antimicrobial activity.

Time Tested Formulations - Founded in 1989 by Dr. Rachel Fresco to address 9 9 yinfection and immune function using botanical medicines.

Great Smokies Diagnostic Labs, now Genova performed over 200,000 sensitivity tests during the 6 years Biocidin was tested as a candidate, and in EVERY test it was the most HIGHLY SENSITIVE substance.

For 26 years, formulas created by BioBotanical Research have been recommended by leading practitioners, authors and laboratories. In 2013 a University of Binghamton study showed effectiveness in addressing Biofilms.

In-Vitro tests have demonstrated effectiveness in the same range as pharmaceuticals used for similar purposes, but without the potential toxicity and consequent side effects.

Dysbiosis and Leaky GutCandida Lyme and co-infectionsSkin infectionsViral conditions

• Broad Spectrum of activity against gram positive and gram negative bacteria, yeast, fungus, parasites and viruses.

• Inhibition of biofilms through more than one mechanism of action.

A t b th t i ll d i th di ti

Ingredients Demonstrated:

Viral conditionsUpper respiratory infectionDental/Gum infectionUrinary tract infectionProstate inflammationAuto-immune diseaseElderly or at risk individualsDuring heavy metal chelationAdjunct to cancer treatmentsAny acute or chronic infection

• Acts both systemically and in the digestive system, and may be used in acute or chronic infection.

• Contains immune system modulators to up-regulate the body’s own defenses.

• Supports clearance of toxins through activation of drainage pathways of the liver and kidneys.

• Reduces inflammation and promotes healing of tissues to reverse damage due to leaky gut.

• a synergistic blend to produce desired results without side effects or stress on organ systems.

These statements have not been evaluated by the Food and Drug Administration (FDA).

These products are not intended to diagnose, treat, cure or prevent any disease.

USP Effectiveness Testing of Biocidin®

Demonstrates inhibitory activity of against both gram positive and gram negative bacteria, yeast and fungus.

Bilberry extract, Grape Seed extract, Milk Thistle, Echinacea, Goldenseal, Shiitake, White Willow, Garlic, Black Walnut (hull and leaf), Raspberry, Fumitory, Gentian, Noni, Tea Tree oil, Galbanum oil, Lavender oil, Oregano oil

TextTextText

• High Oleuropein Potency

• Broad Spectrum Organic Olive Leaf

• Viral conditions• Upper respiratory infection• Lyme and Co-infections• Urinary tract infection• Prostate inflammation• Skin infections inc. MRSA

IngredientsOlive Leaf extract (18-24% Oleuropein)

Garlic, Goldenseal, Milk Thistle, St. John’s Wort, UvaUrsi, American Ginseng, Bladderwrack, Cordyceps,

Dandelion, Noni, White Willow.

• Added Immune Modulators

• Detoxification Factors for Liver and

Kidneys

• Excellent addition to Biocidin for

complicated clinical presentations

• Winter Wellness (prevention or

treatment)

• Available in 60 capsules

• Autoimmune disease• Elderly or at risk individuals• During heavy metal chelation• Adjunct to cancer treatments• Any acute or chronic infection

These statements have not been evaluated by the Food and Drug Administration (FDA). These products are not intended to diagnose, treat, cure or prevent any disease.

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Clinical Recommendations:

General Cleansing

Yeast / Candida

LPS / Bacterial Translocation

Prevention Recovery

Reduce exposure to chemicals

Optimize maternal GI health & detox

Avoid ultrasound & meds

Assess heavy metal burden

Address food sensitivities

Test for gluten and casein

Vaginal delivery & breastfeed

Reduce exposure to toxic metals

Avoid antibiotics

Optimize nutrition and supplement if necessary

Test for gluten and casein intolerance

Identify and resolve deficiencies

Test for and address intestinal dysbiosis and pathogenic biofilm

Meet Cash – 3 years old

Case History: dx – autism @ age 2 Non-verbal Little or no eye contact Most severe symptom whole body dermatitis Most severe symptom – whole body dermatitis

Started around 18 months Itching so severe, his whole body is covered with scabs

and open wounds When he was younger, parents bandaged his arms and

legs Taking Atarax since around 2 years old

Meet Cash – 3 years old

Medication History:

Around 40 vaccine doses

22 rounds of antibiotics for repeated infections

Had been under chirocare for several months – no more ear infections!

Testing

From extensive case history, clinical guess was that symptoms were related to systemic yeast infection and significant allergies

With no insurance and limited funding, we g,started with only 1 test – IgG food allergy

Results – Cash was severely allergic to egg yolk, egg whites, milk, wheat and peanuts

Started elimination diet and probiotics

2 Weeks into Elimination Diet

Parents report that Cash is:

Making eye contact

Babbling

Sleeping better Sleeping better

Has been taken off Atarax! Rash is still present but obsessive itching has subsided.

1 week later, mother sent me pictures showing the amazing skin improvement.

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2 Weeks into Elimination Diet 3 Weeks into Elimination Diet

Rash is virtually GONE.

The HOPE for our future depends on p

changing the current course of

healthcare.

Clinic Tools

•Why are additives used in vaccines?

•Which additives are proven harmful or untested?

•What is thimerosal?

•Is thimerosal still in vaccines?

•Is injecting mercury different than consuming mercury?

•What are the effects of injecting aluminum?

•How can I protect my child and my parentalrights?

BioNutritional Care Training

*Fibromyalgia *Chronic Fatigue *Asthma *Allergies *ADHD *Seizure Disorder *Autism *OCD

*Digestive Disorders *Mental Health Disorders *Behavioral Disorders

*Autoimmune Disorders *Other Chronic Conditions

Resources & References

www.IllnessExplained.comwww BioNutritionalCare comwww.BioNutritionalCare.com

www.BioBotanicalResearch.comwww.HopeForAutism.us

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Autism is PREVENTABLE Autism is REVERSABLE A small group of thoughtful people could change the world.

Indeed it's the onlyIndeed, it's the only thing that ever has.

-Margaret Mead