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TOPOLOGICAL DIFFERENCES BETWEEN BIG BLUESTEM AND SWITCHGRASS Sara Krznarich Northern Arizona University Dr. Nancy Johnson

Sara Krznarich Northern Arizona University Dr. Nancy Johnson

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Page 1: Sara Krznarich Northern Arizona University Dr. Nancy Johnson

TOPOLOGICAL DIFFERENCES BETWEEN BIG BLUESTEM AND

SWITCHGRASS

Sara Krznarich

Northern Arizona University

Dr. Nancy Johnson

Page 2: Sara Krznarich Northern Arizona University Dr. Nancy Johnson
Page 3: Sara Krznarich Northern Arizona University Dr. Nancy Johnson
Page 4: Sara Krznarich Northern Arizona University Dr. Nancy Johnson
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Research Question Are there differences in the topology and

mycorrhizal responses of big bluestem (Andropogon geradii) and switchgrass (Panicum virgatum)?

Photo by Wikimedia Commons

Page 6: Sara Krznarich Northern Arizona University Dr. Nancy Johnson

Purpose

Compare plant adaptations to obtain nutrients

Photo by Wikimedia Commons

Page 7: Sara Krznarich Northern Arizona University Dr. Nancy Johnson

Experimental Design

Factorial greenhouse experiment2 grass species: Big bluestem and

Switchgrass2 mycorrhizal fungi: Gigaspora and Glomus

Photo by Nancy Johnson

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Experimental set-up

Photo by Nancy Johnson

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Experimental set-up

0 months 4 months

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Analysis: Computer Software

WinRhizoRoot topology analysis

JMPSurface areaRoot lengthSpecific root length (length/weight)

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Analysis: WinRhizo

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Analysis: WinRhizo

Big bluestem Switchgrass

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Analysis: WinRhizo

SwitchgrassBig bluestem

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Conclusion

Mycorrhizal treatment: unsuccessful

Surface area: not significantly different

Total root length and specific root length differed significantlyBig bluestem > Switchgrass

Different strategies for nutrient uptake

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Acknowledgements

Dr. Nancy Johnson

Northern Arizona University Space Grant Program

Arizona Space Grant Consortium

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Any Questions?