SANTA CRUZ BIOTECHNOLOGY, INC.

COL1A (COL-1): sc-59772

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

BACKGROUND

The extensive family of COL gene products (collagens) is composed of severalchain types, including fibril-forming interstitial collagens (types I, II, III and V)and basement membrane collagens (type IV), each type containing multipleisoforms. Collagens are fibrous, extracellular matrix proteins with high tensilestrength and are the major components of connective tissue, such as tendonsand cartilage. All collagens contain a triple helix domain and frequently showlateral self-association in order to form complex connective tissues. Severalcollagens also play a role in cell adhesion, important for maintaining normaltissue architecture and function.

CHROMOSOMAL LOCATION

Genetic locus: COL1A1 (human) mapping to 17q21.33; Col1a1 (mouse) mapping to 11 D.

SOURCE

Collagen Type I (COL-1) is a mouse monoclonal antibody raised against fulllength native Collagen Type I of bovine origin.

PRODUCT

Each vial contains 100 µl ascites containing IgG1 with < 0.1% sodium azide.

APPLICATIONS

COL1A (COL-1) is recommended for detection of native Collagen Type I ofmouse, rat and human origin by Western Blotting (non-reducing) (startingdilution to be determined by researcher, dilution range 1:100-1:1000),immunofluorescence (starting dilution to be determined by researcher, dilu-tion range 1:50-1:500), immunohistochemistry (frozen) (starting dilution tobe determined by researcher, dilution range 1:500-1:2000) and solid phaseELISA (starting dilution to be determined by researcher, dilution range 1:30-1:3000); non cross-reactive with Collagen Types II, III, IV, V, VI, VII, IX, Xand XI; may cross-react with connective tissue fibers in acetone-fixed orunfixed frozen sections.

COL1A (COL-1) is also recommended for detection of native Collagen Type Iin additional species, including bovine, porcine, feline and canine.

Suitable for use as control antibody for COL1A1 siRNA (h): sc-44041, COL1A1siRNA (m): sc-44044, COL1A1 shRNA Plasmid (h): sc-44041-SH, COL1A1shRNA Plasmid (m): sc-44044-SH, COL1A1 shRNA (h) Lentiviral Particles: sc-44041-V and COL1A1 shRNA (m) Lentiviral Particles: sc-44044-V.

Molecular Weight of Collagen Type I precursor: 130-140 kDa.

Molecular Weight of mature Collagen Type I: 70-90 kDa.

Positive Controls: Hs68 cell lysate: sc-2230, CCD-1064Sk cell lysate: sc-2263or FHs 173We cell lysate: sc-2417.

STORAGE

For immediate and continuous use, store at 4° C for up to one month. Forsporadic use, freeze in working aliquots in order to avoid repeated freeze/thaw cycles. If turbidity is evident upon prolonged storage, clarify solution by centrifugation.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

DATA

PRODUCT CITATIONS

1. Dooley, S., et al. 2008. Hepatocyte-specific Smad7 expression attenuatesTGF-b-mediated fibrogenesis and protects against liver damage.Gastroenterology 135: 642-659.

2. El-Domyati, M., et al. 2015. Microneedling therapy for atrophic acnescars: an objective evaluation. J. Clin. Aesthet. Dermatol. 8: 36-42.

3. Ekizer, A., et al. 2015. Bone marrow mesenchymal stem cells enhance boneformation in orthodontically expanded maxillae in rats. Angle Orthod. 85:394-399.

4. El-Domyati, M., et al. 2015. The use of Botulinum toxin-a injection forfacial wrinkles: a histological and immunohistochemical evaluation. J.Cosmet. Dermatol. 14: 140-144.

5. El-Domyati, M., et al. 2015. Multiple microneedling sessions for minimallyinvasive facial rejuvenation: an objective assessment. Int. J. Dermatol. 54:1361-1369.

6. Sriram, N., et al. 2015. Epigallocatechin gallate attenuates fibroblastproliferation and excessive collagen production by effectively interveningTGF-b1 signalling. Clin. Exp. Pharmacol. Physiol. 42: 849-859.

7. Kim, Y.M., et al. 2016. Anti-wrinkle effects of a tuna heart H2O fraction onHs27 human fibroblasts. Int. J. Mol. Med. 37: 92-98.

8. Wang, J.R., et al. 2016. Signaling cascades governing cdc42-mediatedchondrogenic differentiation and mensenchymal condensation. Genetics202: 1055-1069.

9. Malik, M., et al. 2016. Gonadotropin-releasing hormone analogues inhibitleiomyoma extracellular matrix despite presence of gonadal hormones.Fertil. Steril. 105: 214-224.

10.Patel, A., et al. 2016. Mifepristone inhibits extracellular matrix formation in uterine leiomyoma. Fertil. Steril. 105: 1102-1110.

PROTOCOLS

See our web site at www.scbt.com for detailed protocols and supportproducts.

COL1A (COL-1): sc-59772. Immunofluorescencestaining of methanol-fixed HeLa cells showingmembrane and cytoplasmic localization.