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STM JOURNALSScientific Technical Medical
Research & ReviewsA Journal of
Biotechnology
(RRJoBT)
ISSN : 2347 - 7245May - August 2014
STM Publication, a strong initiative by Consortium E-Learning Network Private ltd.(Estd. 2006) was launched in the
year 2010 under the support and guidance by our esteemed Editorial and Advisory board members from renowned
institutes.
Objectives of STM Publication(s):
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Research & Reviews : A Journal of Biotechnology
Research & Reviews : A Journal of Biotechnology
(ISSN: 2347-7245)
?Industrial Biotechnology
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Mr. Puneet Mehrotra
Managing Director STM Journals, Consortium eLearning Network Pvt. Ltd.(CELNET)
Noida ,India
Group Managing Editor Dr. Archana Mehrotra
DirectorCELNET, Delhi, India
Puneet Pandeya
ManagerMonika Malhotra
Assistant Manager
Assistant Editors
Aditya Sanyal
Anupama Garg
Himani Pandey
Publication Management Team
Internal Members
External Members
Dr. Bimlesh Lochab
Industrial Tribology Machine Dynamics & Maintenance
Engineering Centre (ITMMEC)
Indian Institute of Technology Delhi, India
Prof. S. Ramaprabhu
Alternative Energy Technology Laboratory,
Department of Physics,
Indian Institute of Technology, Chennai, India
Dr. Rajiv Prakash
School of Materials Science and Technology,
Institute of Technology, Banaras Hindu University,
Varanasi, India
Dr. Rakesh Kumar
Assistant Professor, Department of
Applied Chemistry, BIT Mesra,
Patna, India
Associate Editors
Gargi Asha Jha
Nupur Anand
Priyanka Aswal
Sona Chahal
STM Journal (s) Advisory Board
Dr. Ashish RunthalaLecturer, Biological Sciences Group,
Birla Institute of Technology & Science, Pilani Rajasthan, India.
Dr. Baldev Raj
Former Director, Indira Gandhi Centre for Atomic Research, India.
Chairman, National Institute of Technology Puducherry, India.
Dr. Baskar KaliyamoorthyAssociate Professor, Department
of Civil Engineering National Institute of Technology Trichy, India.
Prof. Bankim Chandra RayProfessor and Head, Department of
Metallurgical and Materials Engineering National Institute of Technology,
Rourkela, India.
Prof. D. N. Rao Professor, Department of Biochemistry,
AIIMS, New Delhi, India.
Prof. Jugal KishoreProfessor, Department of Community
Medicine, Maulana Azad Medical College, New Delhi, India.
Dr. Pankaj PoddarScientist, Physical & Materials ChemistryDivision, National Chemical Laboratory,
Pune, India.
Dr. Hardev Singh VirkProfessor Emeritus, Eternal
University, Baru Sahib, India.
Dr. Nandini Chatterjee SinghAssociate Professor,
National Brain Research Centre, Manesar, India.
Dr. Shankargouda PatilAsst. Prof., Department of Oral
Pathology, KLE Society's Institute of Dental Sciences, Bangalore, India.
Prof. Subash Chandra MishraProfessor, Metallurgical & Materials
Engineering Department, NIT, Rourkela, India.
Prof. Yuwaraj Marotrao GhugalProfessor and Head Department, Govt.College of Engineering Station Road,
Osmanpura, Aurangabad, India.
Prof. Sundara RamaprabhuProfessor, Department of Physics
Indian Institute of Technology Madras, India.
Dr. Shrikant Balkisan DhootHead Research & Development,
Nurture Earth R&D Pvt LtdMIT Campus, Beed bypass road,
Aurangabad, India.
Dr. Rakesh KumarAssistant Professor,
Department of Applied Chemistry, BIT Mesra, Patna, India
Dr. Priyavrat TharejaHead, Materials and Metallurgical
Engineering Department, PEC University of Technology,
Chandigarh, India.
STM Journal (s) Advisory Board
Dr. Lokesh UpadhyayProfessor & Director, Advanced Research
Centre for Indian System of Medicine (ARCISM), PRIST University,
Thanjavur, India.
Shankar Parmeshwar HosmaniSBRR Mahajana First Grade College,
Jayalakshmipuram, Mysore, India.
Dr Krishnan PandianIndian Council of Agricultural Research Central Agricultural Research Institute
Port Blair - 744 101 Andaman and Nicobar Islands, India.
Dr. Rajarshi BanerjeeHaldia Institute of Technology,
Haldia, India.
Editorial Board
Dr. Ramasare Prasad YadavIndian Institute of Technology
Rorkee, India.
Professor Chandrasekaran MuthusamyCochin University of Science and
Technology, India.
Dr. Iqbal AhmadHead, Fibre Toxicology Division Indian
Institute of Toxicology Research Lucknow India.
Dulal PandaProfessor Biosciences & Bioengineering
IIT Bombay, India.
Professor Pramod K. BajpaiThapar University, Patiala, India.
Ashish RunthalaBirla Institute of Technology & Science,
Pilani, India.
Prof. Manchikatla Venkat RajamUniversity of Delhi South Campus
Department of Genetics Benito Juarez Road New Delhi, India.
Dr Bhaskar C BeheraScientist Mycology and Plantpathology group Agharkar Research Institute G. G.
Agarkar Road Pune, India.
Dr. Saber Mohamed Abd-AllahFaculty of Veterinary Medicine, Beni-
Suef University, Egypt.
Professor Satpal Singh BishtRoland Institute of Pharmaceutical
Sciences, India.
Dr. Shrikant Balkisan DhootHead Research & Development, PhD Bioprocess Technology Head, R&D
Nurture Earth R&D Pvt LtdAurangabad Maharashtra
Dr. Subban PatharajanScientist, Centre for Research & Development, PRIST University,
Thanjavur, India.
I take the privilege to present the hard copy compilation for the Volume 4 Issue (2) of Research &
Reviews: A Journal of Biotechnology (RRJoBT). The intension of RRJoBT is to create an
atmosphere that stimulates creativeness, research and growth in the area of Biotechnology.
The development and growth of the mankind is the consequence of brilliant Research done by
eminent Scientists and Engineers in every field. RRJoBT provides an outlet for Research findings
and reviews in areas of Biotechnology found to be relevant for National and International recent
developments & research initiative.
The aim and scope of the Journal is to provide an academic medium and an important reference for
the advancement and dissemination of Research results that support high level learning, teaching and
research in the domain of Biotechnology.
Finally, I express my sincere gratitude and thanks to our Editorial/ Reviewer board and Authors for
their continued support and invaluable contributions and suggestions in the form of authoring write-
ups/ reviewing and providing constructive comments for the advancement of the journals. With
regards to their due continuous support and co-operation, we have been able to publish quality
Research/Reviews findings for our customers base.
I hope you will enjoy reading this issue and we welcome your feedback on any aspect of the Journal.
Dr. Archana Mehrotra
Director
STM Journals
Director's Desk
STM JOURNALS
1. Biosorption and Screening of Lead (Pb) Tolerant Indigenous Staphylococcus aureus Isolated from Tannery Effluent Contaminated Site
Muthukumaran P., Arvind J., Priya M., Divya R., Indhumathi E., Keerthika C. 1
2. Comparative Assessment of Antiproliferative Potential of Triphala and Ashokarishta: Dependence on Dose, Exposure Time and Age of the Preparation Oli N., Desai K, Menon S. 8
3. Phytochemical Screening and Trace Element Analysis of Cryptomeria japonicaS. Priya 17
4. Seasonal Variation and Regional Abundance of Wetland Avian Fauna of Urinji Kulam Pond in Thiruthangal, Viruthunagar DistrictJ.Arun Raj , Sevarkodiyone S. P., Pavaraj M., Balaji S. 21
5. Study on Efflux Pump Inhibition Activity of Marine Actinomycetes against Oflaxcin Resistant M. tuberculosisGajalakshmi P., Raja A., Arthur James R. 25
ContentsResearch & Reviews : A Journal of Biotechnology
RRJoBT (2014) © STM Journals 2014. All Rights Reserved
Research & Reviews: A Journal of Biotechnology ISSN: 2231-3826 (online), ISSN: 2347-7245 (print)
Volume 4, Issue 2
www.stmjournals.com
Biosorption and Screening of Lead (Pb) Tolerant
Indigenous Staphylococcus aureus Isolated from Tannery
Effluent Contaminated Site
Muthukumaran P.*, Arvind J., Priya M., Divya R., Indhumathi E., Keerthika C. Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049,
Tamil Nadu, India
Abstract The present study deals with isolation, identification, and characterization of heavy metal
resistant bacteria isolated from effluent of Ezar Tanneries, Vaniyambadi, Vellore district,
South India. Initially, a total of 11 bacterial isolates were screened on Luria Bertani (LB) agar plates supplemented with 5 mg/L concentration of five different heavy metal ions
such as Cr, Cu, Ni, Pb, and Zn separately. A best microorganism was selected based on high level of heavy metal tolerance. On the basis of morphological, biochemical
characterization, the isolates were identified as Staphylococcus aureus. The identified
isolates were resistant to Cu and Pb. S. aureus showed optimum growth at 30°C and pH 6.5. The identified heavy metal resistant S. aureus could be useful for the bioremediation
of heavy metal contaminated sewage and wastewater.
Keywords: bioremediation, heavy metal, lead, Staphylococcus aureus, tannery
effluent
RRJoBT (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: A Journal of Biotechnology ISSN: 2231-3826 (online), ISSN: 2347-7245 (print)
Volume 4, Issue 2
www.stmjournals.com
Comparative Assessment of Antiproliferative Potential of
Triphala and Ashokarishta: Dependence on Dose,
Exposure Time and Age of the Preparation
Oli N., Desai K, Menon S.* Department of Biochemistry, St. Xavier’s College, Navarangpura, Ahmedabad 380 009, India
Abstract Triphala and Ashokarishta are known to be widely used herbal formulations in
Ahmedabad, of which a few are local brands. Triphala is used by people of all ages for general well being while Ashokarishta is prescribed to women suffering from problems
related to menstruation. Five brands of Tripahala and three brands of Ashokarishta were added to Schizosaccharomyces pombe NCYC 1683 cultures (cell cycle mutants) at
concentrations of 0.5, 1.0, 1.5 and 2.0 mg/mL each of the former and 0.15, 0.3, 0.45 and
0.6 mg/mL of the latter for 3 and 24 h exposure time. These were assessed for viability by MTT assay and total protein content. The viability of cells in most experimental trials
with Triphala was found to be significantly (p < 0.05) higher at 24 h than 3 h exposure
time for all drug concentrations, while in trials with Ashokarishta, the percent cell viability varied with dose but showed no significant change with respect to exposure time.
The antiproliferative potential of the drugs was observed to be at 2 mg/mL (Triphala) and 0.6 mg/mL (Ashokarishta) concentrations on exposure for 24 h. The percent cell viability
at lower doses was high. The correlation of total protein and cell viability varied with
different brands. In case of some of the drugs, the total protein at the effective dose was higher than percent cell viability, while in some cases the total protein was lower than the
percent cell viability. Thus, antiproliferative effect of both formulations is dependent on dose and age of preparation.
Keywords: Triphala, Ashokarishta, Schizosaccharomyces pombe cultures, cell viability,
total protein content, dose dependence and exposure time
RRJoBT (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: A Journal of Biotechnology ISSN: 2231-3826 (online), ISSN: 2347-7245 (print)
Volume 4, Issue 2
www.stmjournals.com
Phytochemical Screening and Trace Element Analysis
of Cryptomeria japonica
S. Priya* P.G. Department of Biotechnology, S.T.E.T. Women’s College, Mannargudi, Tamil Nadu, India
Abstract The present study was aimed to investigate the preliminary phytochemical screening of
the bark of Cryptomeria japonica belonging to family Cupressaceae. The bark of the
plant were subjected to successive soxhlet extraction by continuous hot percolation method using organic solvents of increasing polarity such as ethanol. Both the extracts
were subjected to qualitative phytochemical screening and it showed the presence of
active constituents such as alkaloid, flavonoid, phenol and carbohydrates. Heavy metals and inorganic elements are determined by atomic absorption spectroscopy technique. The
results obtained shows that the extracts contain medicinally important bioactive constituents and also heavy metals present in the plant extracts were within the
permissible limits. The results showed that the plants hold tremendous promise in
providing the variable secondary metabolites and mineral supply that could enhance the curative process of ill health.
Keywords: Cryptomeria japonica, phytochemical screening, heavy metal and
inorganic element analysis
RRJoBT (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: A Journal of Biotechnology ISSN: 2231-3826 (online), ISSN: 2347-7245 (print)
Volume 4, Issue 2
www.stmjournals.com
Seasonal Variation and Regional Abundance of Wetland
Avian Fauna of Urinji Kulam Pond in Thiruthangal,
Viruthunagar District
J.Arun Raj *, Sevarkodiyone S. P., Pavaraj M., Balaji S. Post-graduate and Research Department of Zoology, Ayya Nadar Janaki Ammal College
(Autonomous), Sivakasi–626 124, Tamil Nadu, India
Abstract Wetlands are plays a vital role in the ecosystem. It provides good shelter for many
animals such as, birds, mammals, fishes, insects, etc. It is also maintaining the
biodiversity of flora and fauna. Birds are the most important indicators of the ecosystem. Hence, the present study has been carried out to assess the wetland birds in Thiruthangal
from July 2012 to June 2013. During the study period 32 species of birds belonging 19 families were recorded. Among the 19 families Recurvirostidae, Ciconiidae,
Scolopacidae, Pelecanidae and Podicipedidae were found to be more dominant than
other families.
Keywords: avian fauna, wetland, biodiversity, seasonal variation and aquatic birds.
RRJoBT (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: A Journal of Biotechnology ISSN: 2231-3826 (online), ISSN: 2347-7245 (print)
Volume 4, Issue 2
www.stmjournals.com
Study on Efflux Pump Inhibition Activity of Marine
Actinomycetes against Oflaxcin Resistant M. tuberculosis
Gajalakshmi P.1, Raja A.
2, Arthur James R.
3
1Department of Microbiology, Dhanalakshmi Srinivasan College of Arts and Science for Women,
Perambalur – 621 212, Tamil Nadu, India 2Department of Microbiology, Jamal Mohamed College, Tiruchirapalli, Tamil Nadu, India
3Department of Marine Science, Bharathidasan University, Tiruchirapalli – 620 024, India
Abstract Studies were conducted to isolate marine actinomycetes active against oflaxcin resistant M. tuberculosis. Out of 25 actinomycetes isolates designated as ECRMP 4 showed potent
antimycobacterial activity against all three tested Mycobacterial strains. Representative
bioactive isolate was characterized using phenotypic property and identified as Micropolyspora sp. The zone of inhibition was 20 ± 0.1 mm (62% RIZD) against M.
tuberculosis and 18 ± 0.6 mm (100% RIZD) against OFLR M. tuberculosis. The bioassay
of crude compound prevails that the Rf value of compound is 0.16. In this study we
evaluated the role of the efflux pumps in quinolone resistance by using carbonyl cyanide
m-chlorophenyl hydrazone (CCCP), 2,4-dinitrophenol (DNP) and verapamil against clinical isolate of M. tuberculosis. The MIC levels of OFL showed twofold reductions in
presence of CCCP, verapamil and DNP while in case of ECRMP 4 Micropolyspora sp. metabolite showed fourfold reductions in MIC. These finding suggest the involvement of
active efflux pumps activity of isolated compound from Micropolyspora can be able to
produce noval antimycobacterial molecules.
Keywords: Micropolyspora, oflaxcin, relative zone of inhibition, efflux pump