Question

• How does the addition of alcohol to energy drinks affect the body of a Palaemantes Paludousus?

Purpose

• The reason I am doing this project is because there are too many people dying from the use of these harmful drinks. For the last four years I have been trying to educate children and parents about the use of these drinks through my research. With this experiment I am continuing my development of my education into the field so I can spread the information to others.

Hypothesis

• I think that the addition of alcohol to the energy drink will increase the chances of the heart stopping or some type of Arrhythmia (not normal heart beat rhythm).

Research

Heart

Brain Tissue

Research

• The Ghost Shrimp:• Females are larger than males• Also called glass shrimp because they are

clear• Type of feeder shrimp often used in larger

aquariums to Clean the tank or as fish food• Maximum size is two inches• Palaemantes Paludousus

Research

Smirnoff triple distilled vodka• 40% alc/vol• Made in Norwalk, Connecticut • Distilled from grain• Nutrition facts

-69g calories-0g fat-0g carbs-0g protein

Research

• In 2011, sales of the beverage category of “energy shots” rose to 1 billion dollars.• 5 hour leads the category with increased sales of 45.3%, which

yielded 885.2 million dollars.• The energy drinks category increased 15.7% yielding 6.9 billion dollars

for the 52 weeks prior to May 15, 2011.• Share of volume by region in the U.S. in 2008 : West-42.4%, South-

27.8%, Midwest-18.9%, North East-10.8%.

Materials

• Vodka• Red Bull• Ghost shrimp• Cups• Microscope • Pipettes • Timer • Flashlight

• Graduated cylinder • Slides

Procedure

1. Prepare concoctions by mixing the following amounts of Red Bull with vodka:

-10 mL of Red Bull and 0 mL of vodka: 0%-8.5 mL of Red Bull and 1.5 mL of vodka: 15% -7.5 mL of Red Bull and 2.5 mL of vodka: 25%-5 mL of Red Bull and 5 mL of vodka: 50%-2.5 mL of Red Bull and 7.5 mL of vodka: 75%-1.5 mL of Red Bull and 8.5 mL of vodka: 85%-0 mL of Red Bull and 10 mL of vodka: 100%

Procedure (continued)

1. Remove one ghost shrimp with the net and place it in the cup with a little of its own water.

2. Use microscope to count the heart beats for one minute. 3. Add a drop of a substance in the water.4. Wait one minute. 5. Count the beats for one minute. 6. Repeat steps 1-5 For each trial for each concoction making sure to

record your data after you count each time.7. Do this for the different concoctions of energy drink and vodka.

Graph (0% vodka, 100% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 105 126 138 151 167 189

Trial 2 120 138 149 175 188 199

Trial 3 110 127 153 172 184 193

Average 111.666666666667 130.333333333333 146.666666666667 166 179.666666666667 193.666666666667

25

75

125

175

225

Trial 1 Trial 2 Trial 3 Average

Graph (15% vodka, 85% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 110 96 125 136 144 157

Trial 2 105 116 117 124 133 142

Trial 3 120 135 151 169 174 183

Average 111.6 115.6 131 143 150.3 160.6

10

30

50

70

90

110

130

150

170

190

Trial 1 Trial 2 Trial 3 Average

Graph (25% vodka, 75% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 126 162 166 182 196 210

Trial 2 124 166 192 204 216 224

Trial 3 142 135 164 197 200 208

Average 130.666666666667 154.333333333333 174 194.333333333333 204 214

25

75

125

175

225

Trial 1 Trial 2 Trial 3 Average

Graph (50% vodka, 50% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 162 182 186 194 202 202

Trial 2 154 182 196 204 208 210

Trial 3 170 186 200 0 0 0

Average 158 182 191 199 205 206

Column2 NaN NaN NaN NaN NaN NaN

25

75

125

175

225

Trial 1 Trial 2 Trial 3 Average Column2

Graph (75% vodka, 25% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 168 196 231 210 0 0

Trial 2 178 196 180 96 12 0

Trial 3 172 168 138 130 100 14

Average 172.666 186.666 183 145 37.333 4.666

25

75

125

175

225

Trial 1 Trial 2 Trial 3 Average

Graph (85% vodka, 15% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 174 206 244 0 0 0

Trial 2 178 216 186 142 93 41

Trial 3 182 248 262 180 0 0

Average 178 223.333 230.666 107 31 13.666

25

75

125

175

225

275

Trial 1 Trial 2 Trial 3 Average

Graph (100% vodka, 0% Red Bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 169 204 178 0 0 0

Trial 2 160 191 153 153 144 66

Trial 3 110 90 87 63 50 63

Average 146.333333333333 161.666666666667 139.333333333333 72 64.6666666666667 43

25

75

125

175

225

Trial 1 Trial 2 Trial 3 Average

Conclusion

• This experiment went as I had hypothesized with a few twists and turns. As the alcohol was added to the drinks, the heart beats became sporadic and eventually weakened them to the effects of the energy drink. However, there was one thing that completely surprised me. When the percentage of alcohol started to become greater than the energy drinks, the central area between the eyes where most neurological transmission happens, started swelling and caused an instant death.