8/13/2019 Protein Markers

1/1

L6Pwww.fermentas.com

LabAid

PageRulerPlus Prestained Protein Ladder (10 250 kDa)

* Migration patterns were determined using respective NuPAGEprecast gels.

Note.The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

190

11580

70

30

50

25

15

10

190

115

80

70

30

50

25

15

10

185

115

8065

30

50

25

15

10

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

10

130

250

100

70

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

15

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

11580

65

30

50

25

15

185

11580

65

30

50

25

15

190

1158070

30

50

25

15

10

Migration patterns of PageRulerPlus Prestained Protein Ladder in different electrophoresis conditions

Gel type Tris-Glycine Tris-Acetate* Bis-Tris*

Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%

Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES

Apparent Molecular Weights, kDa

10

20

30

40

50

60

70

80

90

100

%l

enghtofgel