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Lupin protein and dietary fibre fractionation processes and potential for food applications Dr LiHui Liu, May 2016 FOOD AND NUTRITION UNIT Dr LiHui Liu, May 2016

protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Page 1: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applicationsDr LiHui Liu, May 2016

FOOD AND NUTRITION UNIT

Dr LiHui Liu, May 2016

Page 2: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

2 |

Lupin Production and Utilization

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Production:

Contribute to the sustainability of cropping systems

600,000 t/2015, gross value of production at $150 million, mainly in WA  

Australia is the largest lupin producer 

and exporter in the world

Current Utilisation:Mainly animal feed, 

Only 4% for human foods from kernels 

or kernel flour 

(Source from DAF‐WA, 2015)

Page 3: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupins vs other pulses

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

• Australian lupins are natural products high in protein and dietary fibre, very low starch, offering potential health benefits

• Lupin has the lowest GI of any commonly consumed grains and pulses

• Lupin gluten free and non GM

Page 4: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin* – Soy composition (g/100g)

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

LupinSeed        Kernel

SoybeanSeed        Kernel

Seed coat 24‐25 5‐6

Protein 33‐36 38‐45 30‐60

NSP (dietary fibre) 30‐32 32‐37 17‐23

Fat 5‐6 6‐7 17‐28

Ash (minerals) 3‐5 3‐5 4‐5 Un‐change

Soluble carbohydrates

8‐9 10‐12 11‐20

Moisture  7‐9 10‐12 9‐13

* Lupinus angustifolius (ASL) 

LupinSeed        Kernel

SoybeanSeed        Kernel

Seed coat 24‐25 5‐6

Protein 33‐36 38‐45 30‐60

NSP (dietary fibre) 30‐32 32‐37 17‐23

Fat 5‐6 6‐7 17‐28

Ash (minerals) 3‐5 3‐5 4‐5 Un‐change

Soluble carbohydrates

8‐9 10‐12 11‐20

Moisture  7‐9 10‐12 9‐13

Page 5: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin vs Soy

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Protein and FatLupin Protein and Dietary FibreSoy

Soy FoodLupin Feed

Lupin production1999 – 1.6 MT2005 – 0.6 MT

LupinFood

Feed ‐ lupin seeds $250/T

value

Page 6: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

1. Whole seeds or kernels (mainly Asian foods)

Bean sproutsa

Lupin drinksBean curdb

Lupin saucec

Temped

a: Yu, Kyle, Hung, Zeckler 1985; b: Hung, Kyle, Yu 1986; c: Yu, Kyle, Hung, Zeckler 1985; d: Hung, Papolais, Nithianandan, Jian, Versteeg 1990

Page 7: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

7 |

Lupin for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

2. Food ingredients Flour

Protein, $4k ‐ $10k/T

Dietary fibre,  $1.5k – 30k/T

3. Products with lupin ingredients:Noodles, PastasBakery productsSnack foodsDrinksTablets 

Page 8: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin kernel protein and dietary fibre fractions

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Kernels / Kernel flour

Wet extraction

Separation

Lupin proteins  Lupin kernel fibres

Drum drying

Fibre flake

Page 9: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

9 |

Lupin kernel protein and dietary fibre fractions

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Kernels / Kernel flour

Wet extraction

Separation

Separation

Lupin proteins 

Spray drying

Lupin kernel fibres

Soluble proteins

Protein isolate powder 

Protein concentrate powder 

Drum drying

Fibre flake

Protein curd

Page 10: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

10 |

Lupin kernel protein and dietary fibre fractions

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Kernels / Kernel flour

Wet extraction

Separation

Lupin proteins  Lupin kernel fibres

Kernel soluble fibres Kernel insoluble fibres

Protein isolate powder 

Protein concentrate powder

Drum drying

Fibre flake

Spray drying Drum drying

Insoluble fibre flakeSoluble fibre powder

Fibre powder

Grinding

Soluble proteins

Separation

Protein curd 

Spray drying

Page 11: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin kernel protein and dietary fibre fraction process

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

DecanterSeparation

ClarifierSeparation

Drum dryerDrying

UFConcentration

Spray dryerDrying

TankExtraction/Mixing

Page 12: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Lupin kernel protein and dietary fibre as food ingredients

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Spray dried lupin proteins Drum dried Lupin fibre  Ground Lupin fibre 

<425 µm 

Page 13: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

13 | Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Emulsifiers or Protein Additives as high cost dairy protein substitutes for bakery or meat products, even yoghurt or ice‐cream product

Foaming & gelling reagents as egg substitute

High solubility for beverages

Lupin kernel protein and dietary fibre as food ingredients

Protein concentrate 

Protein isolate

Page 14: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

14 | Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Lupin kernel fibres

Soluble fibres Insoluble fibres

Binding reagent for bakery, meat, pasta or soup products

Emulsifiers or Protein Additives as high cost dairy protein substitutes for bakery or meat products, even yoghurt or ice‐cream product

Gelling & binding reagents for drink or meat products

Foaming & gelling reagents as egg substitute

High solubility for beverages

Lupin kernel protein and dietary fibre as food ingredients

Protein concentrate 

Protein isolate Binding reagent for bakery, meat or pasta products

Page 15: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

15 |

Functional properties of lupin kernel fibre

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Oil absorption %Water  absorption %

0

200

400

600

800

1000

1200

Lupin flour Lupin fibre Soy fibre Oat fibre0

100

200

300

400

Lupin flour Lupin fibre Soy fibre Oat fibre

Page 16: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

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Functional properties of lupin kernel fibre

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Swelling power g/g Solubility %

0

10

20

30

40

60 70 80 90

Temperature °C

Lupin flour Lupin fibre Soy fibre

0

10

20

30

40

60 70 80 90

Temperature °C

Lupin flour Lupin fibre Soy fibre

Page 17: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

17 |

Lupin kernel dietary fibre as food ingredient

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Page 18: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

18 |

Lupin kernel protein & dietary fibre as food ingredients

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Breads with lupin proteins

Lupin dietary fibre tablets

Page 19: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

19 |

Lupin kernel dietary fibre as food ingredient

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Fruit juices with lupin dietary fibre

Page 20: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

20 |

Lupin kernel dietary fibre as food ingredient

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Fruit bars with lupin dietary fibre

Page 21: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

21 |

Lupin kernel dietary fibre Market Challenging 

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

(Source: Asia Pacific Food Industry, July 1998)

Australasian Natural Ingredients Ltd, AustraliaGeorge Weston Foods Ltd, Australia

KTS Group – Malaysia

Page 22: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

22 |

Lupin kernel dietary fibre market challenging 

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Page 23: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

23 |

Lupin kernel dietary fibre market challenging 

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Page 24: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

24 |

Lupin kernel dietary fibre market challenging 

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Page 25: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

25 |

Lupin kernel dietary fibre market challenging 

Lupin protein and dietary fibre fractionation processes and potential for food applications

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Page 26: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Future opportunities for lupin proteins

26 |

According to the FAO, the world’s population projected to reach9.6 billion and the world demand for animal‐derived protein is expected to double by 2050

The world will need to find alternative protein sources to meet the nutritional demands without overstressing the environment. Lupin can be an economical source of plant proteins for the world demand.

Page 27: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Future opportunities for lupin dietary fibre

27 |

The Global Insoluble Dietary Fibres Market was valued at USD 2.18 billion in 2015 and is projected to reach USD 3.2 billion by 2020, at a CAGR of 7.8% during the forecast period from 2015 to 2020.

In terms of value, the Asia‐Pacific region is projected to be the fastest‐growing market with a CAGR of 15.5% during the forecast period from 2015 to 2020. China is expected to grow at the highest CAGR of 17.9% by 2020.

Page 28: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Conclusions

28 |

• An economical process could produce both lupin protein anddietary fibre ingredients

• With good functionality and highly favourable nutritionalattributes, the lupin kernel fibre is a new and uniqueingredient in a variety of Asian and Western‐style foodsystems. The initial market response to some conceptproducts was positive at Southeast Asia and Australia.

• To capitalize on the encouraging market response, nutritionprofessionals, food processors, food and ingredient retailersand marketing professionals should join forces to furtherpromote food products from Australian sweet lupin.

Page 29: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu

Acknowledgements

29 |

Financial support:GRDC, AusAID, Victoria Government, ANI,George Weston Foods Ltd, Australia

Many staff members of F&N involved in the projects

Special thanks to Dr Hung TV ‐ senior research scientistfor his work on lupins

Page 30: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

FOOD AND NUTRITION UNIT

Thank you

Page 31: protein and dietary fibre fractionation and for food ... · Lupin protein and dietary fibre fractionation processes and potential for food applications| L Liu • Australian lupins

Lupin protein and dietary fibre fractionation processes and potential for food applications|  L Liu31 |

Breaking News on Food & Beverage Development - North AmericaNY start-up unveils the ultimate plant-based protein snack: BRAMI lupini beans. By Elaine Watson+, 04-Apr-2016

Lupini beans have a nutritional profile to die for. The problem is, most Americans have never heard of them. But that could change rapidly if the New York based entrepreneur behind BRAMI snacking beans gets his way.

BRAMI Inc founder Aaron Gatti told FoodNavigator-USA: “I put a pack in front of an angel investor and he ate it in two minutes”. “There are chickens and eggs everywhere in this industry: “Retailers won’t take you on unless you’ve got a distributor, and distributors won’t take you on unless you’ve got retail buyers. It's the same with money. You can’t raise capital until you show investors real numbers, but you can't show great numbers unless you’ve got capital, and so it goes on”.