hnolog

epmaEw(

R

L

L

d

V

Pw

Q

J

E

AotrimwoesfbpttiIipbTecf

A

u

R

C

C

S

d

V

P

Q

J

E

ItmbtPmf2spnatwpfdtaea

A

u

R

W

W

Wcotton knitted fabrics with an alkaline pectinase from Bacillus subtilis WSHB04-02 by using response surface methodology. Biochem. Eng. J. 34, 107–113.

doi:10.1016/j.jbiotec.2008.07.1180

Abstracts / Journal of Biotec

nhancement such as improved yield, shorter time and more sim-lified procedures over heat-reflux extraction was observed, whichakes MCAE (a combination of physical and chemical method)potential convenient tool for eleutheroside B extraction from

leutherococcus senticosus. Moreover, the favorable solvent isater, making MCAE a less expensive and eco-friendly technique

Liu et al., 2007a,b).

eferences

iu, Y., Jin, L.J., Li, X.Y., Xu, Y.P., 2007a. Application of mechanochemical pretreatment(MCPT) to aqueous extraction of isofraxidin from Eleutherococcus senticosus. Ind.Eng. Chem. Res. 46, 6584.

iu, Y., Jin, L.J., Xu, Y.P., Song, C.N., Wang, X.Y., 2007b. Optimization of themechanochemical assisted extraction of total flavonoids from Eleutherococcussenticosus. Shizhen. Med. Materia. Med. Res. 18, 2889.

oi:10.1016/j.jbiotec.2008.07.1178

6-P-019

roperties of immobilized catalase and its application in cottonet processing

iang Wang ∗, Xuerong Fan, Ping Wang, Chengxuan Li, Li Cui

Key Lab of Science and Technology of Eco-Textile, Ministry of Education,iangnan University, Wuxi 214122, China

-mail address: qiang wang@163.com (Q. Wang).

small dose of catalase is capable of breaking down hydrogen per-xide into water and oxygen, which has been extensively used forhe cleanup of residual hydrogen peroxide left on bleached fab-ic to avoid interfering with subsequent dyeing process in textilendustry (Costa et al., 2002; Chen et al., 2007). Although the enzy-

atic process results in cleaner waste water and could reduceater consumption compared with the traditional cleanup meth-ds, it is necessary to immobilize catalase onto or into a solidco-friendly matrix to increase their thermo-stability, operationaltability and recovery (Seyhan and Alptekin, 2004). Extendingrom our previous work about the optimization of catalase immo-ilization on cotton fabric oxidized with sodium periodate, theroperties of this immobilized catalase and its actual applica-ion were investigated in the present paper. The results indicatehat the thermostability, operational stability and storage stabil-ty of immobilized catalase were higher than those of free one.n addition, metal ions have lower inhibition for the activity ofmmobilized catalase compared to its free counterpart. Kineticarameters of the reaction catalyzed by catalase show that immo-ilized catalase presented higher Km and lower Vm than free one.he dyeing properties of cotton fabrics dyed with bleaching efflu-nt treated with immobilized catalase, such as color difference,olor yield(K/S) and rubbing fastness were close to those dyed withresh water.

cknowledgement

This work was financially supported by Jiangsu Provincial Nat-ral Science Foundation (BK2006020).

eferences

osta, S.A., Tzanov, T., Carneiro, F., Gubitz, G.M., Cavaco-Paulo, A., 2002. Recyclingof textile bleaching effluents for dyeing using immobilized catalase. Biotechnol.Lett. 24, 173–176.

hen, J., Wang, Q., Hua, Z.Z., Du, G., 2007. Research and application ofbiotechnology in textile industries in China. Enzyme Microb. Tech. 40,1651–1655.

y 136S (2008) S496–S505 S503

eyhan, T.S., Alptekin, O., 2004. Immobilization and kinetics of catalase onto mag-nesium silicate. Process Biochem. 39, 2149–2155.

oi:10.1016/j.jbiotec.2008.07.1179

6-P-020

retreatment of cotton knits with pectinase and peracetic acid

iang Wang ∗, Xuerong Fan, Ping Wang, Li Cui

Key Lab of Science and Technology of Eco-Textile, Ministry of Education,iangnan University, Wuxi 214122, China

-mail address: qiang wang@163.com (Q. Wang).

n recent years, more and more enzymes has been tried to replaceraditional chemicals used in textile processing. To date, the enzy-

atic pretreatment of cotton which is referred as bioscouring oriopreparation has become a promising eco-friendly alternativeo chemical scouring in cotton preparation (Wang et al., 2006).ectinases have been proven to be more effective for cotton enzy-atic scouring than other enzymes (Wang et al., 2007a). Extending

rom our previous work on cotton bioscouring (Wang et al., 2006,007a,b), peracetic acid (PAA) bleaching of cotton-knitted fabricscoured with alkali and enzyme individually were carried out in thisresent paper. Effects of alkali and pectinase treatment on white-ess, strength, wetting time of PAA-bleached fabrics were studied,nd the optimum parameters were determined. The results showedhat the optimum PAA-bleaching conditions of bioscoured cottonere similar to that of alkaline scoured cotton. i.e. PAA 8 g/L, sodiumyrophosphate 2 g/L, penetrant DX-2 2 g/L, pH 7, bleaching at 70 ◦Cor 60 min. The dyeing properties of cotton fabrics subjected toifferent pretreatments were also very close to each other. In addi-ion, different bleaching processes of enzyme-scoured cotton weressessed and two-bath bioscouring-PAA bleaching reached the bestffects, followed by one-bath two-step bioscouring-PAA bleachingnd two-bath bioscouring-hydrogen peroxide bleaching.

cknowledgement

This work was financially supported by Jiangsu Provincial Nat-ral Science Foundation (BK2006020).

eferences

ang, Q., Fan, X.R., Gao, W.D., Chen, J., 2006. Characterization of bioscoured cottonfabrics using FT-IR ATR spectroscopy and microscopy techniques. Carbohyd. Res.341, 2170–2175.

ang, Q., Fan, X.R., Hua, Z.Z., Gao, W.D., Chen, J., 2007a. Influence of combinedenzymatic treatment on one-bath scouring of cotton knitted fabrics. Biocatal.Biotransform. 25, 9–15.

ang, Q., Fan, X.R., Hua, Z.Z., Chen, J., 2007b. Optimizing bioscouring condition of