Project 1Project 1Frog Gels: Analysis of SkinFrog Gels: Analysis of Skin

John Donich

Claudia Morales

Kevin Berry

Project description

Goal: to describe the set of proteins in 7.5%

gels and 12 % gels…

Are males differentiable from females…?

Are individuals differentiable?

An ideal model…

There is little variation between frogs of the

same sex

There’s a distinct diference in the set

between sexes

A protein that shows in only one of the

sexes

Method

Measured relative mobility of protien bandsPlotted standards’ relative mobility vs. Log

Molecular WeightChose a model and fit the data Looked for patterns within individuals and

between sexes

Choosing models

Linear Model for 7.5% gels… Only 3 Degrees of Freedom (5 Standards) Linear Model had smallest confidence interval

Cubic Model for 12% gels… 5 Degrees of Freedom (7 Standards) Cubic and Linear models had similar confidence

intervals, but R2-adj better for cubic (99% vs 96%)

Linear Model

y = -0.8192x + 5.4079

R2 = 0.9754

4.5000

4.6000

4.7000

4.8000

4.9000

5.0000

5.1000

5.2000

5.3000

5.4000

0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000

Relative Mobility

Log

Mol

ecul

ar W

eigh

t

Cubic Model

y = -1.838x3 + 3.8188x2 - 3.1736x + 5.4975

R2 = 0.999

4.2000

4.4000

4.6000

4.8000

5.0000

5.2000

5.4000

0.0000 0.1000 0.2000 0.3000 0.4000 0.5000 0.6000 0.7000 0.8000 0.9000 1.0000

relative mobility

Log

mol

ecul

ar W

eigh

t

Conclusions

The variation among individuals is present

The sex of a frog cannot be determined by protein gel analysis

There is no single protein that distinguishes sexes

?