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ProcessesDNA RNA Misc.Protein
What is the base pair rule?Why is it important
ANSWER:
In DNA Adenine always bonds to Thymine
Cytosine always bonds to Guanine
This allows DNA to act as its own template for
replication
The point where the 2 DNA strands separate is called the …
ANSWER:
Replication Fork
What enzyme unzips the DNA molecule?
ANSWER:
Helicase
The strand of DNA where replication happens
continuously is called the …..
ANSWER:
Leading strand
Binding site for RNA polymerase?
ANSWER:
Promoter region
Part of RNA molecule that are spliced back together to form
mRNA
ANSWER:
Exons
How is RNA processed on the 3’ end?
5’ end?
ANSWER:
5’ – methylation capping3’ – polyadenylation (poly A
tail)
What is determines the reading frame?
What can a reading frame shift do?
ANSWER:
Start and stop codonsStart and stop codons
Mutation or it can cause Mutation or it can cause an aborted translation an aborted translation
processprocess
Name three differences between RNA & DNA
ANSWER:
1. DNA Sugar Deoxyribose RNA - ribose
2. DNA double stranded RNA – most single stranded
3. DNA – Thymine RNA - Uracil
Causes translation to end?
“Stop” codon
What enzyme adds nucleotides along a
DNA strand during ?
In what direction does this occur?
ANSWER:
DNA PolymeraseAll DNA synthesis occurs 5'-3'. The original DNA strand must be read 3'-5' to produce a 5'-3' strand.
What are Okazaki fragments?
What enzyme processes them?
ANSWER:
Discontinuous fragments of DNA made on the lagging strand.
Ligase
What amino acid codon initiates translation in eukaryotes?
ANSWER:
AUG
What is the coding unit?
ANSWER:
The section of DNA being copied into mRNA during transcription
What is enzyme A?What does it do?
ANSWER:
Topoisomerase
Unwinds the DNA Strand
What are the 3 steps of translation?
ANSWER:
1- Initiation
2- elongation
3 - termination
What levels of structure are found in functional proteins?
ANSWER:
4 levels
What is the structure of a
ribosome?
ANSWER:
Made from ribosomal RNA and Proteins
Made up of small and large subunits
DAILY DOUBLE
What is an anti codon?Where is it found?What does it do?
ANSWER:Three nucleotides
complementary to a codon on mRNA
Found on tRNA
Deliver amino acids to the ribosome
a.
The two new strands of DNA that result from this process will …… A. have the same nucleotide order.B. have different nucleotide orders.C. result in a strand of mRNA,D. always results in a protein.
ANSWER:
A. have the same nucleotide order.
What is this a picture of?
ANSWER:
mRNA
Is there double stranded RNA?If so what is it?
ANSWER:
tRNA
What is the Central Dogma of Molecular Biology?
ANSWER:
What does semi conservative replication mean?
ANSWER:
The resulting DNA includes one original
strand and one new strand
One original strand is
“conserved” or saved.
What is “anti parallel” mean?
ANSWER:DNA is oriented in
the opposite direction, causing
continuous synthesis ion one strand and
discontinuous on the other
What does RNA primase do?
ANSWER:ANSWER:
Allows the RNA primer to Allows the RNA primer to attach to a parent DNA attach to a parent DNA strand. All primers are strand. All primers are
RNA!!RNA!!