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Pre – AP Biology DNA Biotechnology (4.4) Part 1

Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

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Page 1: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Pre – AP Biology

DNA Biotechnology (4.4)Part 1

Page 2: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Cloning & Genetic recombination

Page 3: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Cutting the DNA sources using Restriction Enzymes

Page 4: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Using Restriction Enzymes to create “stick ends” on DNA molecules

Page 5: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Combining DNA from both sources“Sticky ends” match

Page 6: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Reintroduce the recombined Plasmid

Page 7: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Let the Bacteria grow and reproduce

Page 8: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

The whole process of cloning

Page 9: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Grow bacteria for use

Page 10: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Kary Mullis

Page 11: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Polymerase Chain Reaction

Page 12: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Pre – AP Biology

DNA Biotechnology (4.4)Part 2

Page 13: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

DNA Gel Electrophoresis

Page 14: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

RFLPs and banding

Page 15: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Human Genome Project

Page 16: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Gene Therapy to treat diseases

Page 17: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

New DNA based Medicines

Page 18: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Crime Scene Investigations

Page 19: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Environmental Clean-up in the gulf

Page 20: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Agriculture

Page 21: Pre – AP Biology DNA Biotechnology (4.4) Part 1. Cloning & Genetic recombination

Livestock