Upload
rodney-barton
View
220
Download
2
Tags:
Embed Size (px)
Citation preview
Pop Culture ScienceBio-Rad Biotechnology Explorer IDEA and STEM Kits
Biotechnology Explorer™ | explorer.bio-rad.com2
Curriculum and Training Specialists
Leigh Brown
Sherri Andrews, Ph.D.
Damon Tighe
Biotechnology Explorer™ | explorer.bio-rad.com3
Bio-Rad Resources
Biotechnology Explorer™ | explorer.bio-rad.com4
Workshop Overview
What are we doing? Pop Culture + Lab Techniques
How do we get students engaged? Movie scenarios
Deeper dive into technique
Choose your favorite movie scenario!
Lab tailored to movie pick
Standards alignment
Other ideas?
Results
Biotechnology Explorer™ | explorer.bio-rad.com5
Getting Students Engaged
Biotechnology Explorer™ | explorer.bio-rad.com6
A Few of your Students’ Favorite Movies?
Biotechnology Explorer™ | explorer.bio-rad.com7
Electrophoresis separates molecules by CHARGE and SIZE
Molecular sieve
Electricity
Electrophoresis: “carry with water”
Biotechnology Explorer™ | explorer.bio-rad.com8
Lapse It! (10 second intervals)
Biotechnology Explorer™ | explorer.bio-rad.com9
Interstellar
Link to trailer of Interstellar movie: https://www.youtube.com/watch?v=2LqzF5WauAw
Biotechnology Explorer™ | explorer.bio-rad.com10
Insterstellar
https://www.youtube.com/watch?v=zSWdZVtXT7E
Biotechnology Explorer™ | explorer.bio-rad.com11
Interstellar
Setting the scene: Crop blight has caused a massive die-off of most food crops. You are in a crew of astronauts who travel through a wormhole, searching for a new planetary home for humanity with a suitable food source.
Biotechnology Explorer™ | explorer.bio-rad.com12
Interstellar
Premise: You find some potentially edible specimens from an exoplanet near the wormhole, but some are poisonous, and contain a visible blue dye.
Students must test the potential food source to find out if it contains the poisonous dye.
Biotechnology Explorer™ | explorer.bio-rad.com13
Interstellar
Teacher Prep: – Blue 1 FD&C dye used for a
standard (to represent poisonious blue dye)
– Medicine cup full of M&Ms or other candies (Nerds, Skittles) – students can separate colors out into other cups.
Lab Set Up: Each student has several specimens (M&Ms candy) to test. They use gel electrophoresis to determine if the blue dye is any of the specimens.
poisonous blue dye
potential food sources
Biotechnology Explorer™ | explorer.bio-rad.com14
Divergent
Link to trailer of Divergent movie: http://divergentthemovie.com/
Biotechnology Explorer™ | explorer.bio-rad.com15
Divergent
https://www.youtube.com/watch?v=336qJITnDi0
Biotechnology Explorer™ | explorer.bio-rad.com16
Divergent
Setting the scene: You are a teenager in post-apocalyptic Chicago. Everyone is divided into 5 factions based on their aptitude and personality.
Biotechnology Explorer™ | explorer.bio-rad.com17
Divergent
Premise: During your 16th year, you must decide whether to stay in your family’s faction, or join a different faction and leave your family behind.
There are several ways to determine which faction you belong in, including an aptitude test.
Biotechnology Explorer™ | explorer.bio-rad.com18
Divergent
After your aptitude test, results are now confirmed using a new technique to see what faction you most closely resemble. Do you fit in one faction? Or are you divergent, showing aptitude for 2 or more factions?
**Results for Candor may be inconclusive.
Hint: You might want to set out
bowls of different colors of candies
here for a visual for your students
Biotechnology Explorer™ | explorer.bio-rad.com19
Divergent
Teacher Prep: – M&Ms or Skittles (5 different
colors) to represent each faction
– FD&C dyes for student samples – some should be a mix of 2 or more colors. Others should just be 1 color. Mix 1:1.
Lab Set Up: Students will prepare extracts from the “faction samples” (candy). You might want to have these separated out into colors at the front of the room, each in its own bowl. These extracts will be run on a gel, along with 3 student samples. Students will identify their sample as matching one faction, or multiple ones (i.e. divergent), and decide if additional tests are needed or if the results are conclusive.
student sample B
faction samples
student sample A
student sample C
Biotechnology Explorer™ | explorer.bio-rad.com20
Despicable Me 2
Link to trailer of Despicable Me 2 movie: https://www.youtube.com/watch?v=u6RxZK3LTnA
Biotechnology Explorer™ | explorer.bio-rad.com21
Despicable Me 2
Setting the scene: PX-41 is a dangerous mutagen engineered by villians. It can turn minons into furry killing machines, capable of destroying everything in their paths. You are a reformed evil scientist, looking for an antidote to cure the mutated minions.
Biotechnology Explorer™ | explorer.bio-rad.com22
Despicable Me 2
Premise: Using the materials available to you in your not-so-evil scientist laboratory, hatch a plan to purify different antidotes. You will need to:
1.Identify which samples contain the yellow antidote.
2.Are any of them pure?
3.Can you find another source for the antidote?
Biotechnology Explorer™ | explorer.bio-rad.com23
Despicable Me 2
Teacher Prep: – FD&C dye – yellow,
plus mixes of 2 or more other FD&C dyes (include yellow in a few)
– Optional – M&Ms or Skittles for students to test for the presence of an antidote.
Lab Set Up: Each workstation has a different set of potential antidotes, made from FD&C dyes. One is pure (yellow) – the others are mixes of various dyes.
Sample D
Potential antidote sources (students pick 2
colors to test)
Sample C Sample ESample BSample A
pure antidote sample
Biotechnology Explorer™ | explorer.bio-rad.com24
Before we start…a little background
What is electrophoresis?
Biotechnology Explorer™ | explorer.bio-rad.com25
How can you separate molecules?
Biotechnology Explorer™ | explorer.bio-rad.com26
Food and marker dyes have an intrinsic SIZE and CHARGE and thus can be separated using Electrophoresis
Food dye Molecular weight
Charge
FD&C Blue 1 792.86 -2
FD&C Yellow 5 534.37 -3
FD&C Yellow 6 452.37 -2
FD&C Red 40 496.43 -2
Carminic Acid 492.38 0
Beetroot red 551.48 +1
Insert close up of FD&C dyes in kit
Biotechnology Explorer™ | explorer.bio-rad.com27
DIY vs Commercial Electrophoresis Chamber
Biotechnology Explorer™ | explorer.bio-rad.com28
Time to Pick!
Biotechnology Explorer™ | explorer.bio-rad.com29
Procedure - Interstellar
Before we begin:1.Find the Blue 1 tube in your green rack. This will represent the poisonous blue dye that we are testing for.
2.Locate the potential food sources (M&Ms).
3.Find the Extraction Buffer tubes.
4.We won’t be using any of the other tubes in the rack, so you can put them to the side.
poisonous blue dye
potential food sources
Extraction buffer
Biotechnology Explorer™ | explorer.bio-rad.com30
Procedure - Interstellar
1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose.
2. Center gel in plastic chamber so that the wells are going across the box.
Biotechnology Explorer™ | explorer.bio-rad.com31
Procedure - Interstellar
3. Add enough TAE buffer to completely cover gel.
4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel.
Biotechnology Explorer™ | explorer.bio-rad.com32
Procedure - Interstellar
5. Separate M&Ms by color. Put one candy of each color into each cup.
6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer.
Extraction buffer 250 ul
Biotechnology Explorer™ | explorer.bio-rad.com33
Procedure - Interstellar
7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample.
Lane 1: Blue 1 (poisonous dye)Lane 2: Yellow M&MLane 3: Red M&MLane 4: Brown M&MLane 5: Blue M&MLane 6: Orange M&MLane 7: Green M&M
***note: order is not important – just be sure to write down what you did!
Load 10 ul of each samplet
Biotechnology Explorer™ | explorer.bio-rad.com34
Procedure - Interstellar
8. Assemble your battery tower as shown in the diagram.
9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip won’t fall over.
Biotechnology Explorer™ | explorer.bio-rad.com35
Procedure - Interstellar
10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes.
11. Compare the Blue #1 standard to your food samples, and
see if any contain the poisonous blue dye.
Biotechnology Explorer™ | explorer.bio-rad.com36
Procedure - Divergent
Before we begin1. Remove any green M&Ms from
your candy cup – we won’t need them during the lab. The other colors will represent each faction.
2. Pick one sample (A, B, C, D, or E) for each person at your workstation.
3. Find the Extraction Buffer tubes.
4. We won’t be using any of the other tubes in the rack, so you can put them to the side.
student sample B
faction samples
student sample A
student sample C
Extraction buffer
Biotechnology Explorer™ | explorer.bio-rad.com37
Procedure - Divergent
1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose.
2. Center gel in plastic chamber so that the wells are going across the box.
Biotechnology Explorer™ | explorer.bio-rad.com38
Procedure - Divergent
3. Add enough TAE buffer to completely cover gel.
4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel.
Biotechnology Explorer™ | explorer.bio-rad.com39
Procedure - Divergent
5. You have representative samples from each faction in front of you. Separate these by color, putting one of each color into each cup.
6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer.
Extraction buffer 250 ul
Biotechnology Explorer™ | explorer.bio-rad.com40
Procedure - Divergent
7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample.
Lane 1: Yellow (Abnegation)Lane 2: Red (Amity)Lane 3: Brown (Candor)Lane 4: Blue (Erudite)Lane 5: Orange (Dauntless)Lane 6: Student A sample Lane 7: Student B sample Lane 8: Student C sample
Load 10 ul of each samplet
Biotechnology Explorer™ | explorer.bio-rad.com41
Procedure - Divergent
8. Assemble your battery tower as shown in the diagram.
9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip won’t fall over.
Biotechnology Explorer™ | explorer.bio-rad.com42
Procedure - Divergent
10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes.
11. Compare the student samples to the faction samples. Which faction do you belong to?
Are you divergent?
Biotechnology Explorer™ | explorer.bio-rad.com43
Procedure – Despicable Me
1. Find the following tubes in your green rack:• Yellow 5 (pure antidote sample)• Samples A, B, C, D, and E from
potential antidotes.• Extraction Buffer
2. We won’t be using any of the other tubes in the rack, so you can put them to the side.
3. Decide which 2 colors of candies might be a potential source of the yellow antidote. Take one of each of those 2 colors out, and put the rest of the candies aside.
Sample D
Potential antidote sources
(pick 2 colors to test)
Sample C Sample ESample BSample A
pure antidote sample
Before we begin
Extraction buffer
Biotechnology Explorer™ | explorer.bio-rad.com44
Procedure – Despicable Me
1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose.
2. Center gel in plastic chamber so that the wells are going across the box.
Biotechnology Explorer™ | explorer.bio-rad.com45
Procedure – Despicable Me
3. Add enough TAE buffer to completely cover gel.
4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel.
Biotechnology Explorer™ | explorer.bio-rad.com46
Procedure – Despicable Me
5. You have samples which potentially contain the antidote sitting in front of you. You also have some purified antidote. If you’d like, you may test 2 M&M samples to see if they contain the antidote as well.
6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer.
Extraction buffer 250 ul
Biotechnology Explorer™ | explorer.bio-rad.com47
Procedure – Despicable Me
7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample.
Lane 1: Yellow 5 (Antidote)Lane 2: ALane 3: BLane 4: CLane 5: DLane 6: ELane 7: Yellow M&MLane 8: Green M&M
Load 10 ul of each samplet
Biotechnology Explorer™ | explorer.bio-rad.com48
Procedure – Despicable Me
8. Assemble your battery tower as shown in the diagram.
9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container and the paper clip with the alligator clip ensures that the paper clip won’t fall over.
Biotechnology Explorer™ | explorer.bio-rad.com49
Procedure – Despicable Me
10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes.
11. Compare the purified antidote sample in lane 1 to the potential antidote samples. Which ones contain the antidote? Are any pure?
Biotechnology Explorer™ | explorer.bio-rad.com50
What Can You Teach?
Biotechnology Explorer™ | explorer.bio-rad.com51
Other Ideas?
Biotechnology Explorer™ | explorer.bio-rad.com52
Results - Interstellar
Blu
e #
1
Yel
low
M&
M
Red
M&
M
Bro
wn
M&
M
Blu
e M
&M
Ora
nge
M&
M
Gre
en M
&M
Biotechnology Explorer™ | explorer.bio-rad.com53
Results - Divergent
Yel
low
(A
bneg
atio
n)
Red
(A
mity
)
Bro
wn
(Can
dor)
Blu
e (
Eru
dite
)
Ora
nge
(Da
untle
ss)
Stu
den
t E
Stu
den
t B
Stu
den
t C
Make new gel with Student A (yellow 6), B, E
Biotechnology Explorer™ | explorer.bio-rad.com54
Results – Despicable Me
Ant
idot
e (Y
ello
w 5
)
Sam
ple
A
Sam
ple
B
Sam
ple
C
Sam
ple
D
Sam
ple
E
Yel
low
M&
M
Gre
en M
&M
Biotechnology Explorer™ | explorer.bio-rad.com55
Teacher Prep and Lab Setup – for all 3 scenarios
1. Create the following samples to be used in Despicable Me or Divergent. Mixtures should be equal parts. • A: Yellow 6 • B: Yellow 5 + Yellow 6• C: Yellow 5 + Blue 1 + Red 40• D: Blue 1 + Yellow 6• E: Yellow 5 + Blue 1
2. You will also need the following FD&C dyes:• For Interstellar: Blue 1• For Despicable Me: Yellow 5
3. Each station also should have:• 6 medicine cups• 1 cup with M&Ms (mixed colors)• Micropipet and tips• Dye extraction buffer (2 ml)• Gel• STEM gel box (box, paper clip, alligator clips, batteries)• Sharpie
Biotechnology Explorer™ | explorer.bio-rad.com56
IDEA STEM Electrophoresis Kit
IDEA/STEM Electrophoresis Kits Found in the 2014/2015 Catalog on pg. 48-49