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PharmacopeiaApplication eBookSMALL MOLECULE ANALYSIS

Improve United States Pharmacopeia (USP) Method Performance with Allowable Adjustments

Core-Shell Technology

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2

Isocratic Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) plusmn 30 Relative Cannot exceed plusmn 10 Absolute change Cannot be reduced to zero

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 degC

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate plusmn 50 (at given ID)

Column inner diameter Can be adjusted so long as linear velocity is maintained

Column length Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Particle size Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

Alternatively (as for the application of particle size adjustment to superficially porous particles) other Ldp combinations can be used provided that the number of theoretical plates (N) is within -25 to +50

Gradient Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) Changes to gradient composition are not recommended

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 ˚C

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate Changes to flow rate are not allowed

Column inner diameter Changes to column length particle size or inner diameter are not allowed

Column length Changes to column length particle size or inner diameter are not allowed

Particle size Changes to column length particle size or inner diameter are not allowed

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

This guide provides analysts with solutions to standard USP monographs and allows the incorporation of cutting edge and reproducible HPLC column technology to provide shorter separation times and improved resolution while meeting all the quality standards of the USP for years to come

United States Pharmacopeia Allowable Adjustments General Chapter lt621gt

ON-DEMAND WEBINARApply USP Chapter lt621gt Allowable Adjustments to Your USP Pharmacopeia Method

Walk-through with our scientists

TipReducing excess HPLC system volume will allow you to realize maximum separation power

Phenomenex l WEB wwwphenomenexcomHPLC

Click to View On-Demand Webinar

3

Click for LC Resource Centers and Downloads

Genotoxic Nitrosamine Resource Center

COVID-19 Application Resource Center

New Micro LC Column and Trap Guide

copy 2020 Phenomenex Inc All rights reserved

2

Isocratic Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) plusmn 30 Relative Cannot exceed plusmn 10 Absolute change Cannot be reduced to zero

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 degC

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate plusmn 50 (at given ID)

Column inner diameter Can be adjusted so long as linear velocity is maintained

Column length Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Particle size Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

Alternatively (as for the application of particle size adjustment to superficially porous particles) other Ldp combinations can be used provided that the number of theoretical plates (N) is within -25 to +50

Gradient Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) Changes to gradient composition are not recommended

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 ˚C

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate Changes to flow rate are not allowed

Column inner diameter Changes to column length particle size or inner diameter are not allowed

Column length Changes to column length particle size or inner diameter are not allowed

Particle size Changes to column length particle size or inner diameter are not allowed

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

This guide provides analysts with solutions to standard USP monographs and allows the incorporation of cutting edge and reproducible HPLC column technology to provide shorter separation times and improved resolution while meeting all the quality standards of the USP for years to come

United States Pharmacopeia Allowable Adjustments General Chapter lt621gt

ON-DEMAND WEBINARApply USP Chapter lt621gt Allowable Adjustments to Your USP Pharmacopeia Method

Walk-through with our scientists

TipReducing excess HPLC system volume will allow you to realize maximum separation power

Phenomenex l WEB wwwphenomenexcomHPLC

Click to View On-Demand Webinar

3

Isocratic Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) plusmn 30 Relative Cannot exceed plusmn 10 Absolute change Cannot be reduced to zero

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 degC

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate plusmn 50 (at given ID)

Column inner diameter Can be adjusted so long as linear velocity is maintained

Column length Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Particle size Column length (L) to particle size diameter (dp) ratio can be adjusted between -25 and +50

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

Alternatively (as for the application of particle size adjustment to superficially porous particles) other Ldp combinations can be used provided that the number of theoretical plates (N) is within -25 to +50

Gradient Separations and MethodsComponent United States Pharmacopeia (USP)

Mobile phase minor component (le 50 ) Changes to gradient composition are not recommended

Mobile phase pH plusmn 02 pH units

Buffer concentration plusmn 10

Column temperature plusmn 10 ˚C

Injection volume Can be adjusted as much as needed Must be consistent with linearity precision and detection requirements

Detector wavelength Cannot be modified

Flow rate Changes to flow rate are not allowed

Column inner diameter Changes to column length particle size or inner diameter are not allowed

Column length Changes to column length particle size or inner diameter are not allowed

Particle size Changes to column length particle size or inner diameter are not allowed

Stationary phase No change of the identity of the substituent permitted

Guards Same stationary phase as column Guard ID le column ID Guard length le 15 column length

This guide provides analysts with solutions to standard USP monographs and allows the incorporation of cutting edge and reproducible HPLC column technology to provide shorter separation times and improved resolution while meeting all the quality standards of the USP for years to come

United States Pharmacopeia Allowable Adjustments General Chapter lt621gt

ON-DEMAND WEBINARApply USP Chapter lt621gt Allowable Adjustments to Your USP Pharmacopeia Method

Walk-through with our scientists

TipReducing excess HPLC system volume will allow you to realize maximum separation power

Phenomenex l WEB wwwphenomenexcomHPLC

Click to View On-Demand Webinar

3

4

Pharmacopeia Application Highlights

bull Reduce Run Times Without Re-Validation

bull Achieve Higher Resolution

bull Stay Within Allowable Adjustment

Robust Separation of Hydrochlorothiazide and Chlorothiazide in Hydrochlorothiazide Tablets Using Kinetex 5 microm C18

Limit of Free Salicylic Acid in Aspirin Tablets Using Kinetex 5 microm C18 Under USP Allowable Adjustments

Robust Separation of Hydroxychloroquine and Chloroquine in Hydroxychloroquine Sulfate Tablets Using Kinetex 5 microm C18

Separation of Ibuprofen and Valerophenone Using Kinetex 5 microm C18 and Under USP Allowable Adjustments

Separation of Lovastatin and Related Impurity with Kinetex 5 microm C18 Under USP Allowable Adjustments

Separation of Clavulanate and Amoxicillin Using Kinetex 5 microm C18 and Under USP Allowable Adjustments

Phenomenex l WEB wwwphenomenexcomHPLC

Less Band Broadening

Kinetex Core-Shell

Fully Porous

More Band Broadening

Make the Easy-Switch to Kinetex

Why Choose Kinetex Core-Shell Columnsbull Performance Gains on Any

LC System

bull System-to-System and Lab-to-Lab Reproducibility

bull Improve the Productivity of Older Established Methods

Click Each

Core-Shell Technology

Columns are pH stable from 15-10 under isocratic conditions Columns are pH stable 15-85 under gradient conditions

ACIDIC COMPOUNDS

XB-C18

Si TMSTMS

Kinetex XB-C18

TMSTMS

PolarPolar

pH Range15 ndash 85

USP ClassificationL1

Effective Carbon Load9

pH Range15 - 85

USP ClassificationL1

Effective Carbon Load9

pH Range15 - 85

USP ClassificationL1

Effective Carbon Load10

PolarC18

TMSTMS

PolarPolar

POLAR ACIDS

Lunareg Omega Polar C18Kinetexreg Polar C18

EXTREMELY HYDROPHOBIC

Kinetex C8

C8

TMSTMSTMSTMS

Luna Omega C18

HYDROPHOBIC COMPOUNDS

Kinetex C18

C18

TMSTMS

pH Range15 ndash 85

USP ClassificationL1

Effective Carbon Load11

pH Range15 - 85

USP ClassificationL1

Effective Carbon Load12

pH Range15 - 85

USP ClassificationL7

Effective Carbon Load8

Kinetex EVO C18

EVOC18

TMSTMS

ALKALINE CONDITIONS

TMSTMS

++

Luna Omega PS C18Kinetex PS C18

+ +

PSC18

TMSTMS

POLAR BASES

pH Range1 ndash 12

USP ClassificationL1

Effective Carbon Load11

pH Range15 - 85

USP ClassificationL1

Effective Carbon Load9

pH Range15 - 85

USP ClassificationL1

Effective Carbon Load9

TMSTMS

Biphenyl F5

F

F

F

F

F

TMSTMS

CLOSELY RELATED COMPOUNDS amp AROMATIC HYDROCARBONS

Kinetex Biphenyl Kinetex F5 Kinetex Phenyl-Hexyl

Phenyl-Hexyl

TMSTMS

pH Range15 ndash 85

USP ClassificationL11

Effective Carbon Load11

pH Range15 - 85

USP ClassificationL43

Effective Carbon Load9

pH Range15 ndash 85

USP ClassificationL11

Effective Carbon Load11

AminoAqAq

Amide Polyol

Luna Omega SUGAR

SUGAR

Kinetex HILIC

HILIC

OO

OHH20

H20H20 H20

H20

H20

H20

H20 H20

H20

H20H20

ACN ACN ACN ACN

ACN ACN ACN ACN

ACN ACN ACN ACN

ACN ACN ACN ACN

HO

HO

OHSi

SiSi

HO

OH

EXTREMELY POLAR

Kinetex PAH

PAH

O

SiO

Si

O

SiO

Si

PAH COMPOUNDS

pH Range20 ndash 75

USP ClassificationL3

Effective Carbon Loadmdash

pH Range15 - 85

USP ClassificationL118

Effective Carbon Load12

pH Range20 - 70

USP Classificationmdash

Effective Carbon Loadlt 2

FROM UHPLC TO HPLC

Selectivity Recommendations

5 microm

35 microm

26 microm

17 microm

13 microm

Core-Shell Technology

Kinetex core-shell particle LC

columns provide unparalleled

performance gains on ANY LC

system

Plus you have your pick of 11

amazing stationary phases and 5

versatile particle sizes

Phenomenex l WEB wwwphenomenexcomHPLC 5

6 Phenomenex l WEB wwwphenomenexcomHPLC

17 μm Columns (mm)SecurityGuardtrade

ULTRA CartridgesDagger

Phases 30 x 21 50 x 21 100 x 21 150 x 21 3pkEVO C18 ndashndash 00B-4726-AN 00D-4726-AN 00F-4726-AN AJ0-9298F5 ndashndash 00B-4722-AN 00D-4722-AN 00F-4722-AN AJ0-9322Biphenyl ndashndash 00B-4628-AN 00D-4628-AN 00F-4628-AN AJ0-9209XB-C18 00A-4498-AN 00B-4498-AN 00D-4498-AN 00F-4498-AN AJ0-8782C18 00A-4475-AN 00B-4475-AN 00D-4475-AN 00F-4475-AN AJ0-8782C8 00A-4499-AN 00B-4499-AN 00D-4499-AN 00F-4499-AN AJ0-8784HILIC 00A-4474-AN 00B-4474-AN 00D-4474-AN ndashndash AJ0-8786Phenyl-Hexyl ndashndash 00B-4500-AN 00D-4500-AN 00F-4500-AN AJ0-8788

for 21 mm ID

26 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 21 50 x 21 75 x 21 100 x 21 150 x 21 3pkEVO C18 00A-4725-AN 00B-4725-AN ndashndash 00D-4725-AN 00F-4725-AN AJ0-9298PS C18 00A-4780-AN 00B-4780-AN ndashndash 00D-4780-AN 00F-4780-AN AJ0-8951Polar C18 00A-4759-AN 00B-4759-AN ndashndash 00D-4759-AN 00F-4759-AN AJ0-9532F5 00A-4723-AN 00B-4723-AN ndashndash 00D-4723-AN 00F-4723-AN AJ0-9322Biphenyl 00A-4622-AN 00B-4622-AN ndashndash 00D-4622-AN 00F-4622-AN AJ0-9209XB-C18 00A-4496-AN 00B-4496-AN 00C-4496-AN 00D-4496-AN 00F-4496-AN AJ0-8782C18 00A-4462-AN 00B-4462-AN 00C-4462-AN 00D-4462-AN 00F-4462-AN AJ0-8782C8 00A-4497-AN 00B-4497-AN 00C-4497-AN 00D-4497-AN 00F-4497-AN AJ0-8784HILIC 00A-4461-AN 00B-4461-AN 00C-4461-AN 00D-4461-AN 00F-4461-AN AJ0-8786Phenyl-Hexyl 00A-4495-AN 00B-4495-AN 00C-4495-AN 00D-4495-AN 00F-4495-AN AJ0-8788

for 21 mm ID

26 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 30 50 x 30 75 x 30 100 x 30 150 x 30 3pkEVO C18 ndashndash 00B-4725-Y0 ndashndash 00D-4725-Y0 00F-4725-Y0 AJ0-9297PS C18 00B-4780-Y0 00D-4780-Y0 ndashndash 00D-4780-Y0 00F-4780-Y0 AJ0-8950Polar C18 ndashndash 00B-4759-Y0 ndashndash 00D-4759-Y0 00F-4759-Y0 AJ0-9531F5 ndashndash 00B-4723-Y0 ndashndash 00D-4723-Y0 00F-4723-Y0 AJ0-9321Biphenyl ndashndash 00B-4622-Y0 ndashndash 00D-4622-Y0 00F-4622-Y0 AJ0-9208XB-C18 00A-4496-Y0 00B-4496-Y0 00C-4496-Y0 00D-4496-Y0 00F-4496-Y0 AJ0-8775C18 00A-4462-Y0 00B-4462-Y0 00C-4462-Y0 00D-4462-Y0 00F-4462-Y0 AJ0-8775C8 00A-4497-Y0 00B-4497-Y0 00C-4497-Y0 00D-4497-Y0 00F-4497-Y0 AJ0-8777HILIC 00A-4461-Y0 ndashndash ndashndash ndashndash 00F-4461-Y0 AJ0-8779Phenyl-Hexyl ndashndash 00B-4495-Y0 ndashndash 00D-4495-Y0 00F-4495-Y0 AJ0-8781

for 30 mm ID

26 microm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 46 50 x 46 75 x 46 100 x 46 150 x 46 250 x 46 3pkEVO C18 ndashndash 00B-4725-E0 ndashndash 00D-4725-E0 00F-4725-E0 00G-4725-E0 AJ0-9296PS C18 00B-4780-E0 00D-4780-E0 00F-4780-E0 00G-4780-E0 AJ0-8949Polar C18 ndashndash 00B-4759-E0 ndashndash 00D-4759-E0 00F-4759-E0 00G-4759-E0 AJ0-9532F5 ndashndash 00B-4723-E0 ndashndash 00D-4723-E0 00F-4723-E0 00G-4723-E0 AJ0-9320Biphenyl ndashndash 00B-4622-E0 ndashndash 00D-4622-E0 00F-4622-E0 00G-4622-E0 AJ0-9207XB-C18 ndashndash 00B-4496-E0 00C-4496-E0 00D-4496-E0 00F-4496-E0 00G-4496-E0 AJ0-8768C18 00A-4462-E0 00B-4462-E0 00C-4462-E0 00D-4462-E0 00F-4462-E0 00G-4462-E0 AJ0-8768C8 ndashndash 00B-4497-E0 00C-4497-E0 00D-4497-E0 00F-4497-E0 00G-4497-E0 AJ0-8770HILIC ndashndash 00B-4461-E0 00C-4461-E0 00D-4461-E0 00F-4461-E0 00G-4461-E0 AJ0-8772Phenyl-Hexyl ndashndash 00B-4495-E0 00C-4495-E0 00D-4495-E0 00F-4495-E0 00G-4495-E0 AJ0-8774

for 46 mm ID

5 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 50 x 30 100 x 30 150 x 30 3pkEVO C18 00B-4633-Y0 00D-4633-Y0 00F-4633-Y0 AJ0-9297F5 00B-4724-Y0 00D-4724-Y0 00F-4724-Y0 AJ0-9321Biphenyl 00B-4627-Y0 00D-4627-Y0 00F-4627-Y0 AJ0-9208XB-C18 00B-4605-Y0 00D-4605-Y0 00F-4605-Y0 AJ0-8775C18 00B-4601-Y0 00D-4601-Y0 00F-4601-Y0 AJ0-8775C8 00B-4608-Y0 00D-4608-Y0 ndashndash AJ0-8777Phenyl-Hexyl 00B-4603-Y0 00D-4603-Y0 ndashndash AJ0-8781

for 30 mm ID

17 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 30 50 x 30 100 x 30 3pkXB-C18 00A-4498-Y0 00B-4498-Y0 00D-4498-Y0 AJ0-8775C18 ndashndash 00B-4475-Y0 00D-4475-Y0 AJ0-8775C8 00A-4499-Y0 00B-4499-Y0 00D-4499-Y0 AJ0-8777HILIC ndashndash 00B-4474-Y0 ndashndash AJ0-8779

for 30 mm ID

17 μm Columns (mm)Phases 50 x 10 100 x 10 150 x 10EVO C18 00B-4726-A0 00D-4726-A0 00F-4726-A0Biphenyl 00B-4628-A0 00D-4628-A0 00F-4628-A0

5 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 50 x 46 100 x 46 150 x 46 250 x 46 3pkEVO C18 00B-4633-E0 00D-4633-E0 00F-4633-E0 00G-4633-E0 AJ0-9296F5 00B-4724-E0 00D-4724-E0 00F-4724-E0 00G-4724-E0 AJ0-9320Biphenyl 00B-4627-E0 00D-4627-E0 00F-4627-E0 00G-4627-E0 AJ0-9207XB-C18 00B-4605-E0 00D-4605-E0 00F-4605-E0 00G-4605-E0 AJ0-8768C18 00B-4601-E0 00D-4601-E0 00F-4601-E0 00G-4601-E0 AJ0-8768C8 00B-4608-E0 00D-4608-E0 00F-4608-E0 00G-4608-E0 AJ0-8770Phenyl-Hexyl 00B-4603-E0 00D-4603-E0 00F-4603-E0 00G-4603-E0 AJ0-8774

for 46 mm ID

13 μm Columns (mm)Phases 30 x 21 50 x 21C18 00A-4515-AN 00B-4515-AN

Dagger SecurityGuard ULTRA Cartridges require holder Part No AJ0-9000

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26 μm Micro LC Columns (mm)Phases 30 x 03 50 x 03 100 x 03 150 x 03 50 x 05 150 x 05Biphenyl 100 Aring ndashndash 00B-4622-AC ndashndash 00F-4622-AC 00B-4622-AF mdashC18 100 Aring ndashndash 00B-4462-AC ndashndash 00F-4462-AC 00B-4462-AF mdashEVO C18 100 Aring ndashndash 00B-4725-AC ndashndash 00F-4725-AC 00B-4725-AF mdashF5 100 Aring ndashndash 00B-4723-AC 00D-4723-AC 00F-4723-AC 00B-4723-AF mdashXB-C18 100 Aring 00A-4496-AC 00B-4496-AC ndashndash 00F-4496-AC 00B-4496-AF 00F-4496-AF

New Dimensions

Ordering InformationCore-Shell Technology

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17 μm Columns (mm)SecurityGuardtrade

ULTRA CartridgesDagger

Phases 30 x 21 50 x 21 100 x 21 150 x 21 3pkEVO C18 ndashndash 00B-4726-AN 00D-4726-AN 00F-4726-AN AJ0-9298F5 ndashndash 00B-4722-AN 00D-4722-AN 00F-4722-AN AJ0-9322Biphenyl ndashndash 00B-4628-AN 00D-4628-AN 00F-4628-AN AJ0-9209XB-C18 00A-4498-AN 00B-4498-AN 00D-4498-AN 00F-4498-AN AJ0-8782C18 00A-4475-AN 00B-4475-AN 00D-4475-AN 00F-4475-AN AJ0-8782C8 00A-4499-AN 00B-4499-AN 00D-4499-AN 00F-4499-AN AJ0-8784HILIC 00A-4474-AN 00B-4474-AN 00D-4474-AN ndashndash AJ0-8786Phenyl-Hexyl ndashndash 00B-4500-AN 00D-4500-AN 00F-4500-AN AJ0-8788

for 21 mm ID

26 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 21 50 x 21 75 x 21 100 x 21 150 x 21 3pkEVO C18 00A-4725-AN 00B-4725-AN ndashndash 00D-4725-AN 00F-4725-AN AJ0-9298PS C18 00A-4780-AN 00B-4780-AN ndashndash 00D-4780-AN 00F-4780-AN AJ0-8951Polar C18 00A-4759-AN 00B-4759-AN ndashndash 00D-4759-AN 00F-4759-AN AJ0-9532F5 00A-4723-AN 00B-4723-AN ndashndash 00D-4723-AN 00F-4723-AN AJ0-9322Biphenyl 00A-4622-AN 00B-4622-AN ndashndash 00D-4622-AN 00F-4622-AN AJ0-9209XB-C18 00A-4496-AN 00B-4496-AN 00C-4496-AN 00D-4496-AN 00F-4496-AN AJ0-8782C18 00A-4462-AN 00B-4462-AN 00C-4462-AN 00D-4462-AN 00F-4462-AN AJ0-8782C8 00A-4497-AN 00B-4497-AN 00C-4497-AN 00D-4497-AN 00F-4497-AN AJ0-8784HILIC 00A-4461-AN 00B-4461-AN 00C-4461-AN 00D-4461-AN 00F-4461-AN AJ0-8786Phenyl-Hexyl 00A-4495-AN 00B-4495-AN 00C-4495-AN 00D-4495-AN 00F-4495-AN AJ0-8788

for 21 mm ID

26 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 30 50 x 30 75 x 30 100 x 30 150 x 30 3pkEVO C18 ndashndash 00B-4725-Y0 ndashndash 00D-4725-Y0 00F-4725-Y0 AJ0-9297PS C18 00B-4780-Y0 00D-4780-Y0 ndashndash 00D-4780-Y0 00F-4780-Y0 AJ0-8950Polar C18 ndashndash 00B-4759-Y0 ndashndash 00D-4759-Y0 00F-4759-Y0 AJ0-9531F5 ndashndash 00B-4723-Y0 ndashndash 00D-4723-Y0 00F-4723-Y0 AJ0-9321Biphenyl ndashndash 00B-4622-Y0 ndashndash 00D-4622-Y0 00F-4622-Y0 AJ0-9208XB-C18 00A-4496-Y0 00B-4496-Y0 00C-4496-Y0 00D-4496-Y0 00F-4496-Y0 AJ0-8775C18 00A-4462-Y0 00B-4462-Y0 00C-4462-Y0 00D-4462-Y0 00F-4462-Y0 AJ0-8775C8 00A-4497-Y0 00B-4497-Y0 00C-4497-Y0 00D-4497-Y0 00F-4497-Y0 AJ0-8777HILIC 00A-4461-Y0 ndashndash ndashndash ndashndash 00F-4461-Y0 AJ0-8779Phenyl-Hexyl ndashndash 00B-4495-Y0 ndashndash 00D-4495-Y0 00F-4495-Y0 AJ0-8781

for 30 mm ID

26 microm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 46 50 x 46 75 x 46 100 x 46 150 x 46 250 x 46 3pkEVO C18 ndashndash 00B-4725-E0 ndashndash 00D-4725-E0 00F-4725-E0 00G-4725-E0 AJ0-9296PS C18 00B-4780-E0 00D-4780-E0 00F-4780-E0 00G-4780-E0 AJ0-8949Polar C18 ndashndash 00B-4759-E0 ndashndash 00D-4759-E0 00F-4759-E0 00G-4759-E0 AJ0-9532F5 ndashndash 00B-4723-E0 ndashndash 00D-4723-E0 00F-4723-E0 00G-4723-E0 AJ0-9320Biphenyl ndashndash 00B-4622-E0 ndashndash 00D-4622-E0 00F-4622-E0 00G-4622-E0 AJ0-9207XB-C18 ndashndash 00B-4496-E0 00C-4496-E0 00D-4496-E0 00F-4496-E0 00G-4496-E0 AJ0-8768C18 00A-4462-E0 00B-4462-E0 00C-4462-E0 00D-4462-E0 00F-4462-E0 00G-4462-E0 AJ0-8768C8 ndashndash 00B-4497-E0 00C-4497-E0 00D-4497-E0 00F-4497-E0 00G-4497-E0 AJ0-8770HILIC ndashndash 00B-4461-E0 00C-4461-E0 00D-4461-E0 00F-4461-E0 00G-4461-E0 AJ0-8772Phenyl-Hexyl ndashndash 00B-4495-E0 00C-4495-E0 00D-4495-E0 00F-4495-E0 00G-4495-E0 AJ0-8774

for 46 mm ID

5 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 50 x 30 100 x 30 150 x 30 3pkEVO C18 00B-4633-Y0 00D-4633-Y0 00F-4633-Y0 AJ0-9297F5 00B-4724-Y0 00D-4724-Y0 00F-4724-Y0 AJ0-9321Biphenyl 00B-4627-Y0 00D-4627-Y0 00F-4627-Y0 AJ0-9208XB-C18 00B-4605-Y0 00D-4605-Y0 00F-4605-Y0 AJ0-8775C18 00B-4601-Y0 00D-4601-Y0 00F-4601-Y0 AJ0-8775C8 00B-4608-Y0 00D-4608-Y0 ndashndash AJ0-8777Phenyl-Hexyl 00B-4603-Y0 00D-4603-Y0 ndashndash AJ0-8781

for 30 mm ID

17 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 30 x 30 50 x 30 100 x 30 3pkXB-C18 00A-4498-Y0 00B-4498-Y0 00D-4498-Y0 AJ0-8775C18 ndashndash 00B-4475-Y0 00D-4475-Y0 AJ0-8775C8 00A-4499-Y0 00B-4499-Y0 00D-4499-Y0 AJ0-8777HILIC ndashndash 00B-4474-Y0 ndashndash AJ0-8779

for 30 mm ID

17 μm Columns (mm)Phases 50 x 10 100 x 10 150 x 10EVO C18 00B-4726-A0 00D-4726-A0 00F-4726-A0Biphenyl 00B-4628-A0 00D-4628-A0 00F-4628-A0

5 μm Columns (mm)SecurityGuard

ULTRA CartridgesDagger

Phases 50 x 46 100 x 46 150 x 46 250 x 46 3pkEVO C18 00B-4633-E0 00D-4633-E0 00F-4633-E0 00G-4633-E0 AJ0-9296F5 00B-4724-E0 00D-4724-E0 00F-4724-E0 00G-4724-E0 AJ0-9320Biphenyl 00B-4627-E0 00D-4627-E0 00F-4627-E0 00G-4627-E0 AJ0-9207XB-C18 00B-4605-E0 00D-4605-E0 00F-4605-E0 00G-4605-E0 AJ0-8768C18 00B-4601-E0 00D-4601-E0 00F-4601-E0 00G-4601-E0 AJ0-8768C8 00B-4608-E0 00D-4608-E0 00F-4608-E0 00G-4608-E0 AJ0-8770Phenyl-Hexyl 00B-4603-E0 00D-4603-E0 00F-4603-E0 00G-4603-E0 AJ0-8774

for 46 mm ID

13 μm Columns (mm)Phases 30 x 21 50 x 21C18 00A-4515-AN 00B-4515-AN

Dagger SecurityGuard ULTRA Cartridges require holder Part No AJ0-9000

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26 μm Micro LC Columns (mm)Phases 30 x 03 50 x 03 100 x 03 150 x 03 50 x 05 150 x 05Biphenyl 100 Aring ndashndash 00B-4622-AC ndashndash 00F-4622-AC 00B-4622-AF mdashC18 100 Aring ndashndash 00B-4462-AC ndashndash 00F-4462-AC 00B-4462-AF mdashEVO C18 100 Aring ndashndash 00B-4725-AC ndashndash 00F-4725-AC 00B-4725-AF mdashF5 100 Aring ndashndash 00B-4723-AC 00D-4723-AC 00F-4723-AC 00B-4723-AF mdashXB-C18 100 Aring 00A-4496-AC 00B-4496-AC ndashndash 00F-4496-AC 00B-4496-AF 00F-4496-AF

New Dimensions

Ordering InformationCore-Shell Technology

Terms and Conditions Subject to Phenomenex Standard Terms and Conditions which may be viewed at wwwphenomenexcomTermsAndConditions

Trademarks Kinetex and Luna are registered trademarks and SecurityGuard and BE-HAPPY are trade-marks of Phenomenex

Disclaimer Comparative separations may not be representative of all applications

Kinetex EVO is patented by Phenomenex US Patent Nos 7563367 and 8658038 and foreign counterparts

FOR RESEARCH USE ONLY Not for use in clinical diagnostic procedures

copy 2020 Phenomenex Inc All rights reserved

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