Peroxidase lab

What is peroxidase?All organisms have enzymes (catalysts) called peroxidases that break down hydrogen peroxide into less harmful substance. Hydrogen Peroxide (H2O2) is a pale blue liquid that appears clear when diluted. Hydrogen peroxide is a weak acid that is used as a bleaching agent and disinfectant.

Do Learn ConnectDo: Peroxidase Lab

Learn: I can identify the products of a reaction and classify reactions in terms of energy.

Connect: Properties and Patterns, Energy

Question:Will chicken liver react with hydrogen peroxide and if so what products are formed?

HypothesisIf chicken liver reacts with hydrogen peroxide, then I will observe __________ (chemical reaction clues) and ____________ will be formed (products) because ________________________________________________________________________________________________

MaterialsChicken LiverHydrogen PeroxideErlenmeyer FlaskStopperToothpickCandle

Procedure Add Hydrogen Peroxide to flask. Stopper the flask. Record observations

Remove stopper from flask and insert toothpick into neck of flask. Do not drop it.

Record observations

Stopper flask. Use the candle to ignite the toothpick, blow out the

flame. Remove stopper from flask and insert toothpick into

neck of flask. Do not drop it.

Procedure Add a small amount of chicken liver to the flask. Stopper the flask. Record observations.

After about 2 minutes, carefully feel the bottom of the flask. Record Observations

Ignite the toothpick and blow out the flame. Remove stopper and insert toothpick into the neck of the

flask. Do not drop it. Record observations.

Stopper the flask and repeat the last steps three more times.

Consider these questions:Why did you make observations after each step?

What observations indicated that a chemical change took place? (Clues)

Classify the reaction as endothermic or exothermic using evidence.

What products were formed in this reaction?

Why is it important to replace the stopper quickly when it had been removed?

ProductsThe equation for the reaction is :

2H2O2 ________ and ________

CER (Conclusion)Claim: The chicken liver did react with the hydrogen peroxide forming ______________. (Products)

Evidence: During the chemical reaction, I observed ________________. (Clues)

Reasoning: Provide your own reasoning connecting your products and clues to chemical reactions. Pre-Ap should also connect to ENERGY.