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57

Lampiran 1 Gambar ampas buah merah (a) dan ransum percobaan (b)

(a) (b) Lampiran 2 Perbandingan lemak pada gizzard ayam perlakuan kontrol (a) dan

perlakuan ABM 1.5% (b)

(a)

(b)

Lampiran 3 Gambaran histopat villi usus halus ayam yang diberi perlakuan ABM

1.5% (a), dan ayam perlakuan tanpa ABM (b)

(a)

(b)

58

Lampiran 4 Komposisi zat gizi buah merah dan ampas buah merah

Senyawa Aktif Kandungan Buah Merah1 Ampas Buah Merah2

Energi 394 kalori/100 gram 6 316 kal/g Protein 3 300 mg/100 gram 3.11 % Lemak 28 100 mg/100 gram 7.6 % Kalsium 54 000 mg/100 gram 0.14 % Fosfor 30 mg/100 gram 0.89 % Besi 2.44 mg/100 gram - Vitamin B1 0.9 mg/100 gram - Vitamin C 25.7 mg/100 gram - Nialin 1.8 mg/100 gram -

Sumber : 1 Budi dan Paimin (2005) 2 Analisis Laboratorium Ilmu dan Teknologi Pakan Fapet IPB, 2009 - Tidak dianalisis Lampiran 5 Analisis ragam konsumsi ransum kumulatif Sumber db JK KT F P Perlakuan 4 105608 26402 0.97 0.454 Error 15 409378 27292 Total 19 514987 Level N Rataan Stdev T0 4 3148.5 275.4 T1 4 2987.3 117.2 T2 4 2990.8 138.4 T3 4 3097.4 105.5 T4 4 2961.5 128.9 Lampiran 6 Analisis ragam pertambahan bobot badan Sumber db JK KT F P Perlakuan 4 46881 11720 0.61 0.664 Error 15 289485 19299 Total 19 336365 Level N Rataan Stdev T0 4 1639.6 200.9 T1 4 1596.3 117.3 T2 4 1593.8 167.9 T3 4 1639.8 70.7 T4 4 1507.2 95.6

59

Lampiran 7 Analisis ragam konversi ransum Sumber db JK KT F P Perlakuan 4 0.0227 0.0057 0.55 0.705 Error 15 0.1562 0.0104 Total 19 0.1789 Level N Rataan Stdev T0 4 1.92 0.0718 T1 4 1.87 0.1228 T2 4 1.88 0.1609 T3 4 1.88 0.0287 T4 4 1.96 0.0714 Lampiran 8 Analisis ragam bobot badan akhir Sumber db JK KT F P Perlakuan 4 55134 13784 0.64 0.642 Error 15 322864 21524 Total 19 377998 Level N Rataan Stdev T0 4 1826.5 218.7 T1 4 1779.1 121.7 T2 4 1769.1 170.6 T3 4 1828.1 75.8 T4 4 1683.9 100.8 Lampiran 9 Analisis ragam persentase karkas Sumber db JK KT F P Perlakuan 4 27.44 6.86 2.22 0.116 Error 15 46.40 3.09 Total 19 73.84 Level N Rataan Stdev T0 4 65.400 1.831 T1 4 67.050 1.859 T2 4 65.650 2.288 T3 4 68.550 1.063 T4 4 67.475 1.513

60

Lampiran 10 Analisis ragam persentase kematian Sumber db JK KT F P Perlakuan 4 80.0 20.0 0.38 0.823 Error 15 800.0 53.3 Total 19 880.0 Level N Rataan Stdev T0 4 7.500 9.574 T1 4 2.500 5.000 T2 4 5.000 10.000 T3 4 2.500 5.000 T4 4 2.500 5.000 Lampiran 11 Analisis ragam indeks performa Sumber db JK KT F P Perlakuan 4 3755 939 0.77 0.562 Error 15 18296 1220 Total 19 22050 Level N Rataan Stdev T0 4 289.46 46.43 T1 4 295.15 35.08 T2 4 281.53 42.61 T3 4 301.56 18.00 T4 4 261.89 23.93 Lampiran 12 Analisis ragam luas permukaan vili usus Sumber db JK KT F P perlak 4 2.860 0.715 4.36 0.016*

Error 15 2.463 0.164 Total 19 5.323 Level N Rataan Stdev T0 4 1.8225 0.4802 T1 4 2.5843 0.5876 T2 4 2.8257 0.2406 T3 4 2.7158 0.3422 T4 4 2.8353 0.2647

61

Lampiran 13 Analisis ragam jumlah butir darah merah hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

2.0713 2.1988 1.8588 2.3267 2.2925

0.288 0.322 0.071 0.291 0.239

0.2475 -

14

T0 T1 T2 T3 T4

2.0400 2.0133 2.2383 1.8962 1.8712

0.098 0.121 0.056 0.076 0.101

0.0058

B B A B B

21

T0 T1 T2 T3 T4

2.1813 2.1350 2.2363 2.0550 2.1650

0.197 0.102 0.326 0.134 0.187

0.9092 -

28

T0 T1 T2 T3 T4

2.4250 2.2950 2.0633 2.1517 2.1488

0.235 0.142 0.147 0.087 0.124

0.2550 -

62

Lampiran 14 Analisis ragam kadar hemoglobin hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

8.3000 9.1200 7.8100 8.6825 8.3350

1.049 1.021 0.263 0.392 0.631

0.3194 -

14

T0 T1 T2 T3 T4

7.5325 9.2200 8.2433 8.1775 8.2875

0.645 1.958 0.370 0.565 0.743

0.4530 -

21

T0 T1 T2 T3 T4

8.7400 8.6233 8.0225 9.6267 9.4025

0.507 0.164 0.855 1.050 0.857

0.1724 -

28

T0 T1 T2 T3 T4

8.0600 8.1667 9.0000 9.1000 8.1950

0.990 0.846 0.688 1.005 0.584

0.4924 -

63

Lampiran 15 Analisis ragam kadar hematokrit hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

22.188 26.250 23.125 26.333 24.250

1.849 1.335 1.635 1.841 1.335

0.0306

B A B A

AB

14

T0 T1 T2 T3 T4

23.500 25.000 23.250 24.063 24.250

0.935 1.275 0.890 1.279 2.143

0.7076 -

21

T0 T1 T2 T3 T4

24.000 23.417 24.938 24.333 24.813

1.551 0.312 1.717 1.230 0.925

0.6792 -

28

T0 T1 T2 T3 T4

23.625 24.750 24.250 24.313 24.000

2.125 0.612 2.131 2.204 1.541

0.9827 -

64

Lampiran 16 Analisis ragam jumlah butir darah putih hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

17.950 37.450 30.750 26.267 23.550

8.319 7.327 11.501 1.087 8.087

0.1031 -

14

T0 T1 T2 T3 T4

26.050 21.800 17.750 14.350 12.900

2.798 3.766 9.314 1.982 3.612

0.0332

A AB AB B B

21

T0 T1 T2 T3 T4

29.450 23.133 16.350 9.600 16.050

4.822 5.367 4.055 2.786 3.456

0.0014

A AB BC C

BC

28

T0 T1 T2 T3 T4

22.000 22.067 15.333 16.600 18.500

2.800 5.144 2.510 2.271 6.077

0.4282 -

65

Lampiran 17 Analisis ragam jumlah heterofil hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

6.625 12.712 13.534 10.333 7.968

4.861 7.969 5.812 2.144 2.222

0.4631 -

14

T0 T1 T2 T3 T4

7.305 5.712 5.036 3.133 1.992

3.698 0.693 2.530 0.842 0.580

0.0685 -

21

T0 T1 T2 T3 T4

5.034 7.846 5.186 3.803 6.366

1.658 3.877 2.771 2.644 2.283

0.5838 -

28

T0 T1 T2 T3 T4

11.784 11.709 6.429 10.818 9.858

1.608 3.592 0.772 2.304 3.256

0.2936 -

66

Lampiran 18 Analisis ragam jumlah limfosit hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

9.708 21.085 15.781 10.759 14.028

3.484 8.540 6.047 2.794 5.896

0.2064 -

14

T0 T1 T2 T3 T4

17.249 15.885 15.712 9.842 9.584

2.337 5.768 5.147 0.826 3.450

0.0846 -

21

T0 T1 T2 T3 T4

17.626 11.895 8.607 4.949 7.421

1.907 4.904 4.091 0.387 4.980

0.0165

A AB B B B

28

T0 T1 T2 T3 T4

8.512 9.497 8.255 4.745 7.819

1.408 2.029 1.761 2.317 3.301

0.4004 -

67

Lampiran 19 Analisis ragam rasio H/L hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

0.6820 0.6030 0.8553 0.9598 0.5685

0.4993 0.2799 0.2869 0.6919 0.1814

0.660 -

14

T0 T1 T2 T3 T4

0.4243 0.3603 0.3208 0.3183 0.2083

0.3109 0.2747 0.0745 0.0977 0.1113

0.655 -

21

T0 T1 T2 T3 T4

0.2858 0.6600 0.6025 0.7683 0.6638

0.0820 0.5117 0.5533 0.4371 0.4474

0.606 -

28

T0 T1 T2 T3 T4

1.3838 1.2330 0.7793 2.2803 1.2605

0.0377 0.2354 0.2292 0.3556 0.7923

0.002

B B B A B

68

Lampiran 20 Analisis ragam titer ND hari ke- 7, 14, 21, 28 setelah perlakuan

Hari ke- Perlakuan Rataan Stdev Sig. Notasi huruf Uji lanjut

7

T0 T1 T2 T3 T4

4.6668 4.4168 3.0625 3.2918 3.4165

0.4714 0.3193 0.1250 0.3437 0.4195

0.000

A A B B B

14

T0 T1 T2 T3 T4

4.4168 4.0003 4.2500 4.7503 4.5000

0.5001 0.4714 0.6313 0.8333 0.7933

0.594 -

21

T0 T1 T2 T3 T4

4.3333 3.9168 4.2500 3.9165 4.3335

0.4714 0.3188 0.3193 0.4195 0.6085

0.459 -

28

T0 T1 T2 T3 T4

4.2498 4.2500 3.4165 3.8333 4.8333

0.8333 0.5691 1.0670 0.6386 0.4303

0.141 -