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OSMOSIS LABOr
Fresh Water vs. Sea Water
PROCEDURE
Write down Procedure (Notebook
pg. 32)
1.Make Table #1 (Notebook pg. 32)
2.Fill out table #1 with class
3.Make table #2 (Notebook pg. 33)
PROCEDURE
4. Make Hypothesis for each cup
(What will happen to the length of
the potato?)
5. Measure potato slices carefully (Put
in table #2)
6. Make sea water solutions (LABEL)
7. Drop potato slices into sea water
solutions and cover with plastic
wrap
PROCEDURE
MAKE SURE TO GET
THE CORRECT POTATO
INTO THE CORRECT
SOLUTION
FRESH WATER VS. SEA WATER
A shipwrecked
sailor is stranded
on a small desert
island with no
fresh water to
drink.
FRESH WATER VS. SEA WATER
She knows she could
last without food for up
to a month, but if she
didn’t have water to
drink she would be
dead within a week.
FRESH WATER VS. SEA WATER
Hoping to
postpone the
inevitable,
her thirst
drove her to
drink the
salty
seawater.
FRESH WATER VS. SEA WATER
She was dead in two days.
FRESH WATER VS. SEA WATER
Why do you think
drinking
seawater killed
the sailor faster
than not drinking
any water at all?
FRESH WATER VS. SEA WATER
Today we explore the cause of
the sailor’s death. We’ll
prepare solutions of salt
water to represent the sea,
and we’ll cut up slices of
potato to represent the sailor.
FRESH WATER VS. SEA WATER
Potatoes are made of cells, as is the
sailor!
OBJECTIVE
To determine the cause of the sailor’s death, we will
determine the effects of salt water on slices of
potato. Our assumption is that potato cells will
behave like the sailor’s cells in her body. We will
compare salt water to fresh water so we can see if
there is any effect caused by the salt in the water.
The fresh water acts as a control in this experiment.
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
5% Sea Water
1% Sea Water
Fresh Water
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
100 ml
5% Sea Water
100 ml
1% Sea Water
100 ml
Fresh Water
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
100 ml
5% Sea Water
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
100 ml
5% Sea Water
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml 100 ml
5% Sea Water
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml
90 ml 100 ml
5% Sea Water
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml
90 ml
100 ml
5% Sea Water
5 ml
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml
90 ml
100 ml
5% Sea Water
5 ml
95 ml
100 ml
1% Sea Water
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml
90 ml
100 ml
5% Sea Water
5 ml
95 ml
100 ml
1% Sea Water
1 ml
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 1
Percent Sea Water
Vol. of 100% Salt Water (ml)
Vol. of Fresh Water (ml) Total Vol. (ml)
10% Sea Water
10 ml
90 ml
100 ml
5% Sea Water
5 ml
95 ml
100 ml
1% Sea Water
1 ml
99 ml
100 ml
Fresh Water
0 ml
100 ml
100 ml
TABLE 2Sample Initial
Length (Day 1)
Final Length (Day 2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
cm.
5% Salt cm.
1% Salt cm.
Fresh Water
cm.
HYPOTHESIS
1. I think that the 10% salt water solution will cause the
potato slice to…
2. I think that the 5% salt water solution will cause the potato
slice to…
3. I think that the 1% salt water solution will cause the potato
slice to…
4. I think that the fresh water solution will cause the potato
slice to…
OSMOSIS LAB DAY 2
(Put this information on pg. 35 in your notebook)
1.Collect cups
2.Remove potato slices carefully
3.Measure slices accurately
4.Fill in Data Table #2
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
cm.
5% Salt
cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
5% Salt
cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm.
5% Salt
cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
5% Salt
cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
X
5% Salt
cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
X
5% Salt
3.8 cm.
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
X
5% Salt
3.8 cm.
4.0 cm
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
X
5% Salt
3.8 cm.
4.0 cm
+ .2 cm
1% Salt
cm.
Fresh Water
cm.
DATA TABLE 2
Sample
Initial Lengt
h (Day
1)
Final Lengt
h (Day
2)
Change in
Length
Tonicity
Hypo- Iso- Hyper-
10% Salt
4.2 cm.
3.9 cm
- .3 cm
X
5% Salt
3.8 cm.
4.0 cm
+ .2 cm
X
1% Salt
cm.
Fresh Water
cm.
OSMOSIS LAB DAY 2
5. Throw potato slices and plastic wrap in
garbage
6. Dump out cups and stack on lab table
7. Answer questions on last page of lab handout
(notebook pg. 34)
8. Make Bar Graph of results from Data Table
#2 for Day 1 and Day 2 potato lengths – put
in notebook pg. 34 or 35
BAR GRAPH
10% Salt 5% Salt 1% Salt Fresh0
0.51
1.52
2.53
3.54
4.5 4.23.8 4 43.9 4 3.9 4
InitialFinal
FORMAL LAB REPORT
• Rough draft – Written in notebook
• Final draft – Written in the following format
FINAL DRAFT
PROBLEM:
1. The problem we are trying to solve is…
2. This problem is important because…
3. The information that is important to know before
we begin is…
4. The control in this experiment is…A. This is the control in the experiment
because…
FINAL DRAFT
PROBLEM:
5. My hypothesis was…A. I made the hypothesis that the 10% salt solution
water would cause the potato slice to…B. I made the hypothesis that the 5% salt solution
water would cause the potato slice to…C. I made the hypothesis that the 1% salt solution
water would cause the potato slice to…D. I made the hypothesis that the fresh water would
cause the potato slice to…
PROCEDURE
1. Materials List – List all materials used in the
experiment.
2.List in order how the experiment was performed.A. The first thing I had to do was ____________B. The next thing I had to do was ____________C. After that I ________________D. Finally I _______________
RESULTS
1.What quantitative and qualitative observations were
you able to make? (list)• Quantitative observations are number type
observations. • Qualitative observations are opinions or color
observations.
2. Data table (taken from your notebook)
3. Graph
OBSERVATIONS AND CONCLUSIONS
1. When the potato slices sat in the water overnight they
_______________A. I think the potato slices did this because ____________
2.Is diffusion, osmosis, or active transport responsible for the
changes in the slices?A. Define:
a. Diffusionb. Osmosisc. Active transport
OBSERVATIONS AND CONCLUSIONS
3. The ______ solution is isotonic to potato cells.A. I know that the solution _______ is isotonic to the potato cells because _____________
4.Define:A. HypertonicB. HypotonicC. Isotonic
OBSERVATIONS AND CONCLUSIONS
5. The sailor died more quickly drinking salt water than
not drinking any water at all because _______________.
6. I think the sailor died from ____________
7. My hypothesis was correct. OR My hypothesis was
not correct.A. My hypothesis was correct because __________. OR My hypothesis was not correct because ____________.