Organ Donor Infectious Disease Testing Education. UW Health OPO July 2009. UW health OPO performs the following infectious disease testing in compliance with the United Network for Organ Sharing (UNOS) standards for every donor:. HIV I/II HTLV I/II Anti-HBc (Hepatitis B Core) - PowerPoint PPT Presentation
Organ Donor Infectious Disease Testing EducationUW Health OPOJuly 2009
UW health OPO performs the following infectious disease testing in compliance with the United Network for Organ Sharing (UNOS) standards for every donor:HIV I/IIHTLV I/IIAnti-HBc (Hepatitis B Core)HBsAg (Hepatitis B surface antigen)HCV (Hepatitis C)VDRL/RPR (Syphilis)CMV (Cytomegalovirus)EBV (Epstein-Barr Virus)
Current ChallengesEarly 2008 Abbott labs discontinued FDA approved kit used by UWHC for Hepatitis B, Hepatitis C & HIVUW Health OPO given exemption by UNOS to perform diagnostic testing, Centaur
Current StatusDonor serological testing is generally done at UW via diagnostic method.Only exception is in the case of a potential islet donor. These panels are sent to BCW (BloodCenter of Wisconsin) for FDA approved serologies.July 1, 2009 All donor serological testing will be done at BCW, including NAT and WNV. The only blood testing done at UW will be ABO verification, EBV (send out) and HLA.
BloodCenter of WisconsinOpen since 1947 and located in Milwaukee, WI On call staff enables donor testing 24 hrs a day 365 days a yearApproved for NAT testing on all donors
NAT TestingThe industry standard is changing with regard to infectious disease testing.NAT (Nucleic Acid Testing) detects the genetic sequence of the virus, bacterium or parasite that is present in the host as a result of the infection.It is used for HIV, HCV, HBV and West Nile Virus.NAT shortens the window of detection for HIV & HCV from approximately 22 days to 5 days and for HBV and WNV to 1 day.Currently 45 out of the 58 OPOs are performing NAT.
New ProcessInitial donor blood draw will now need to be shipped in 2 packagesPackage 1sent to BloodCenter of WI3 7 ml EDTA (lavender) tubes2 10 ml serum sample (red topNOT serum separator) tubesPackage 2sent to UW4 10 ml ACD (yellow top) tubes1 5 or 7 ml lavender tubes
New ProcessFor each bagAffix a label including patients first and last name, date of birth, date and time of draw and initials of person drawingPlace the tubes in a biohazard bag and then package in a small box or envelopeWrite: To BloodCenter of WI on package 1 To UW Hospital ER on package 2
New ProcessTake samples to your ER triageA courier will be arranged to transport the samples from your ER to their destination
Please call organ procurement coordinator on call with any questions: 608-262-2122
New TimelineOnce blood arrives to BCW serology testing takes 4-6 hrs to complete; if any results are positive the test needs to be re-run and take an additional 4-6 hrsOnce blood arrives to UW ER ABO takes under an hourEBV needs to be sent out and results arrive in 5 to 7 daysHLA (tissue typing) takes about 4 hours