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Olympus Vanox Universal Research Microscope (1983)

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Page 1: Olympus Vanox Universal Research Microscope (1983)
Page 2: Olympus Vanox Universal Research Microscope (1983)
Page 3: Olympus Vanox Universal Research Microscope (1983)
Page 4: Olympus Vanox Universal Research Microscope (1983)

AFullLineup ofHighPerformanceand NewlyFormulated LB Objectives

3

Available In four series-S Plan Apochro­mat, S Plan Achromat. 0 Plan Achroma!and D Achromat-the new Olympus lBobjectives make It possible to select justthe right objective combinations dictatedby requirement and budget.S Plan Apochromats are hlgh-qualltyobJectives, fully chromatically corrected,with high resolving power and highcontrast and which feature a partlcularilylarge numerical aperture. They also havesuperior field flatness and may be used Insuper widefield microscopy. This series ofobjectives is ideal for top level researchwork.The S Plan Achromat series of objectivesoffers great versatility as well as highperformance. In addition to normal mien:>scopy, these objectives are well suitedto super widefield and differential inter­ference contrast microscopy. The idealobjectives for various types of microscopy,the S Plan Achromat series makes forhighly efficient research work.The 0 Plan Achromat series does notfeature the super widefield and differentialInterterence contrast observation capa­bilitles of the S Plan Achromat series andthis makes it possible to offer them aslow cost objectives which stili retainexcellent field flatness In normal obser­vation (FN 20). Ideal for general researchwork or for photomicrography.There is also the 0 Achromat series Inwhich the emphasis has been placedsquarely on economy.

Page 5: Olympus Vanox Universal Research Microscope (1983)

Features of LB Objectives

High Resolving PowerTo improve the resolving power of anobjective, the numerical aperture must beincreased. On average, the numericalaperture In the upper range of theOlympus LB series of objectives is some20% hIgher than before.

Excellent ContrastHigh resolving power counts for nothing ifcontrast Is poor. Multilayer lens coatingand other design features of the OlympusLB series of objectives increase contrastconsiderably and yield even sharper andbrighter images.

Superior Field FlatnessThe Olympus LB Plan series objectivesboast superior field flatness, resulting ina 100% effective field, for observation orphotography. Field flatness Is particularlyoutstanding in the S Plan Apochromatand S Plan Achromat series wlch give aconsistently sharp image from edge toedge, even In super widefield observation.

Extended Working DistanceIn the LB series of objectives, Olympushas Increased the working distance asmuch as possible to prevent oil from 011­immersion objectives fOUling other objec­tives, and to facilitate the marking andmanipulation of specimens.

Parlocality with Ultra-lowMagnification ObjectivesBy designing the LB objective series witha 45mm parfocal distance, Olympus hasrealized parfocality with ultra-low magnifi­cation objectives and other objectives.Easier to use and no more troublesomerefocusing!

Increased Field of ViewThe standard WHK tOX eyepiece, which iscorrected so that It matches the LB objec­tive series perfectly, has a field number of2O-an Increase of 23% compared withconventional eyepieces. Moreover, thehigh eyepolnt design of the eyepieceeliminates the need to remove spectacleswhen observing, providing even greaterease of operation.

Page 6: Olympus Vanox Universal Research Microscope (1983)

Features of the VANOX Microscope

5

Observation Tube Height AdjustableThe three-position selector turret makes itpossible to adjust the heIght of the obser­vation tubes for different microscopymethods, Including fluorescence, phasecontrast, polarized light and NomarskiDIG.S: standard, biological microscopyMP: metallurgical, polar/Zing and reflected

light fluorescenceFe: transmitted light fluorescence and

Nomarskl DIG microscopy; for usewith Intermediate magnificationchanger

FocusingCoaxial coarse and fine focus knobs.Coarse focus range, when set for stand­ard biological observation, Is up to26.5mm while the flne focus fange Is2.3mm, with 0.001 mm divisions. A coarselocus stop means fast refocusing, evenafter adjusting the stage height andprevents accidental contact betweenobjective and specimen.

Observation TubeThe AH-BI binocular obervation lube withphoto tube is supplied as standard.Constant tube length adjustment meansthere is no loss of focus when the Inter·pupillary distance is altered. With asuitable framing eyepiece it is thuspossible to use the binocular eyepiecetubes to focus when photographing orframing. A three-way optical path selectormakes it possible to select 100% of thelight for observation, 80% for observationand 20% for photography, or 100% forphotography.

Revolving NosepieceThe revolving nosepiece has openings forfive objectives and is tilted away from theobserver. Optionally available is a sextuplerevolving nosepiece.

Page 7: Olympus Vanox Universal Research Microscope (1983)

StageThe l7Ommx172mm stage rotatesthrough 300 degrees and has a crosstravel motion of 76mm x52mm by meansof the low positioned vertical coaxialspecimen control knobs. The stage designallows the stage Insert to be changed fordifferent methods of microscopy, andInserts for biological, metallurgical andfluorescence microscopy are available.

CondensersANA 1.40 achromatldaplanatlc con­denser Is standard on the VANOX modeland In conjunction with the reversibleauxiliary lens provides Ideal Illuminationfrom magnifications from 4X to max.Achromatldaplanatic condensers arefitted with a slider for oblique illumination.A swlng-out condenser is available forultra-low magnification objectives_

Ught SourceA 12V 100w halogen bulb provides all theillumination needed for any microscopicmethod. It also serves as the light sourcefor reflected light illumination.

TransformerThe transformer is built into the base andcan be adjusted from a to 12 volts. Ideallysuited to nearly every microscopic method.

Page 8: Olympus Vanox Universal Research Microscope (1983)

Phase Contrast Attachment AH-PC-LB

7

Phase contrast Is one of the mostcommon microscopic methods employedIn the study of live material, permittingthe Internal structure of transparentmatter to be observed under differentdegrees of contrast.-The VANOX phase contrast attachmentcomprises a phase contrast turretcondenser, phase contrast objectivesand the eT-S focusing telescope.

-The phase contrast turret condenser hasa NA 1.40 and Is of the achromatlcJaplanatlc design, with a graduatedaperture diaphragm. There are individu­ally ceotarable annular diaphragms forlOX, 2OX, 40X and 1(X)X objectives and Italso Incorporates a darkfield stop(NA 0.911.40) and an empty aperture forbrlghtfleld observation.

-The CT-S centering telescope makes itpossible to center the objective phaserings and condenser annular diaphragmssimply and accurately.

-The phase contrast objectives whichmake up the attachment are available intwo series: PC S Plan Achromat and PCo Achromat, both of which are availableIn either positive or negatIve contrasts.Using the PCS Plan series In conjunctionwith the super widefield attachmentmakes super wIdefield phase contrastmicroscopy possIble.

Page 9: Olympus Vanox Universal Research Microscope (1983)

Differential Interference Contrast Attachmentafter Nomarski AH-NIC-LB

OK; lntermecllale Anachmenl

OIC Condenser·

Phase Contrast ObJ8Clive

BH2·NA

AH·N(;2

PCSPL10XPL

PCSPL40XPl

AH-N!C-LB

Differential Interference contrast (OIC)microscopy makes it possible to observeunstained specimens and live cells undera variety of Interference colors giving arelief-like Image which Is very sharp andclear. This method of microscopy haswide medical and biological applications,such as in distinguishing spindle threadsIn the cell division process, bone mem­brane and nerve tissues.-The VANOX differential Interferencecontrast attachment comprises anIntermediate atlachment, a turretcondenser, a centering telescope andphase contrast objectives.

-The ole turret condenser has a NA 1.40and is an achromaUaplanat with a gradu­ated aperture diaphragm. It featuresNomarskl prisms for tOx, 2OX, 40X andlOQX objectives, annular diaphragms forlOX and 40X phase contrast objectivesand an empty aperture for brightfleldmicroscopy.

-Because standard S Plan Achromatobjectives can be used with the DICattachment, It Is not necessary topurchase special DIC obJectives. Inaddition, super widefield differential Inter·ference contrast microscopy Is possibleby using the DIG attachment in conJunc­tion with the super widefield atU..::hmenl.

-Merits of using differential InterferenceIn conjunction with phase contrast:1. If focusing and positioning of the

specimen is difficult in differentialinterference contrast, phase contrastheips.

2. It is easy to compare the resuftsobtained by the two methods withouthaving to change the condenser.

8

Page 10: Olympus Vanox Universal Research Microscope (1983)

Reflected Light Fluorescence AttachmentAH-RFL-LB

9

In reflected light fluorescence microscopy,the objective serves as its own condenserand this results In the following advan­tages not available with transmittedfluorescence microscopy: fjrst, excitinglight strikes only the field of observation,thus preventing fading a large area of thefluorescent specimen; second, a verybright and sharp fluorescence Image Isobtained because the numerical aper­ture of the objective lens may be used toits fullest advantage.-The reflected light fluorescence Illuml·nator features a quintuple revolvingnosepiece, field diaphragm, aperturediaphragm, exciting filler turret, dichroicmirrors and matching barrier filters.

-The exciting filter turret Incorporatesfour exciting fitters for the U, V, BandG ranges, and four matching dichroicmirrors and barrier filters.

-With U and V excitation, UVFL non-fluo­rescing objectives are recommended.These are available in magnifications of10X, 2OX, 4OX, 40X (olQ and 100X (oll~

The UVFL series of objectives are semi·apochromals, corrected for greatersharpness while the higher than normalnumerical apertures contribute bothto greater sharpness and Increasedbrightness of the fluOfescent Images.

Page 11: Olympus Vanox Universal Research Microscope (1983)

Carclna-embryonlc antigens on thesurface of GAC.J stain the cells.

Page 12: Olympus Vanox Universal Research Microscope (1983)

li'ansmitted Light Fluorescence AttachmentAH-FL-2

"

Transmitted fluorescence microscopy iswidely used in research and general workin the medical, biological, pharmacolo­gical and chemical fields.-The mirror housing features an opticalpath selector, making it possible toswitch over from reflected tight fluo­rescence to transmitted light fluores­cence without having to change the lightsource.

-The light source Is a DC super high­pressure mercury lamp which has a tonglife and provides stable, flicker-freeexcitation.

-Exciting and barrier filters are availablefor U, V and B wavelength excitatronmicroscopy.

File Filter Set (Optional)

Module F·FITC

4 5Barner FIller

BH·FA 10IAttachment

40·FITCW45 043·BG14·3·W45 0

EXCIting Filler 43-BG38·2W4543-BG38-4-W4S

UV Cut-ofl Filler 43-UVCW45

Barrier Filler 20-0515-W 0

Page 13: Olympus Vanox Universal Research Microscope (1983)

VetO cells Inlecl9d wllh measles virus.

"

Page 14: Olympus Vanox Universal Research Microscope (1983)

\l Thtombosls of the cystic artery (HE ,f,ln).S P~n 4)(, NFl< 3.3X.

'1 Pencrutlc c.rc/~ cell, (G/emu ."in).S Pia" 20)(, NFl< 3.3X.

T'MlIf11lIoblBStic~/..S PI,".fOx,. NFK 3.3X.

• e.tl. of mllmmfjty r;arr:Jnoma (Pe".n/colflOCJ ,tain).S Pia" 'Ox, Nfl< 3.3X.Hepatoe/rthosl. (Mflnon-Goidman ",lnJ,S Pie" Apo 4CX. NFl< 2.5".

<t:,.,denocarclnomflln sputum,S Pie" .fOX, NFl< 3.5X.

et!FI!IIBllung flbroofuls (Nomarslfl).S Pis" 10X, NFK 5.DX.

(f)GaBlric mucosa' eire/noma Bnd biopsy of mucosal c16/ecl (HE staIn).S PI,n 2X, NFl< 3.3X.

"

I -.

••.'

-•

....

Page 15: Olympus Vanox Universal Research Microscope (1983)
Page 16: Olympus Vanox Universal Research Microscope (1983)

OtherAttachments

15

Super Widefield AUachment AH-5W·LB-The combination of the SWK lOX superwidefield eyepiece and the AH-$W·TRsuper widefield trinocular tube gives afield nearty twice as wide as customary(FN 26.5).

-Constant tube length adjustment meansthere Is no loss of focus and parfocalltywhen the Interpupillary distance Ischanged.

-A three-way optical path selector makesit possible to switch between 100% ofthe light fOf photography, 100% forobservation, Of &1% for photographyand 20"10 for observation.

-Either S Plan Apochromat or S PlanAchromat objectives may be used withthe attachment and In conjuntlon withthe super widefield condenser, whichIs standard, magnifications of 4X totOOX are possible In super widefield.

Standard Outfits

Modu~ AH·SW.LB

Super Wodeheld AH.swmTrinocular Tube

Super WidelleldAH-SWC 0Condenser

SUper WidefieldSWK lOx .QEyepiece (2 pes,)

High Resolution ProfecUon ScreenAH.sPS-Glare has been eliminated from thishigh resolving power screen whichpermits observation over long periodswithout eye-strain.

-The screen has an effective diameter of155mm;screen magnification =objective powerx NFK eyepiece power x 3

Projection Screen AH·MPS-With the AH·MPS projection screen theemphasis is placed on economy. It hasthe same screen diameter and screenmagnification as the AH-SPS.

Page 17: Olympus Vanox Universal Research Microscope (1983)

Intermediate AUachment MagnificationChanger BH2·CA-Makes It possible to alter the totalmagnlflcatlon of the system in threesteps-lX, 1.25X and 1.5X-wlthouthaving to change eyepIece or objective.

Dry Darkfield Condenser AH·OCD-This dry type darkfleld condenser hasa NA of 0.8 to 0.92 and Is suitable lorobjectives from lOX to 4OX.

Darkfleld Condenser AH·OCW-A cardioId type oil immersion widefielddarkfleld condenser with a NA of 1.20 to1.40, It Is suitable for objectives of 10Xto looX. l00X objectives should have abuilt-in Iris diaphragm.

Swlng-out Condenser AH-5ClB-The top element of this NA 0.85 to 0.05condenser can be swung out. SuitablefOf use with objectives from ultra-lowmagnifications (1)(, 2X) through to loox..

"

Page 18: Olympus Vanox Universal Research Microscope (1983)

Photomicrographic and CinemicrographicSystemCamera PM-lOAD

"

The Model PM·lOAD Is a universal, lullymodular mulliformat camera system withautomatic controls. A built·ln micro­computer assures precision exposure andreadout, reciprocity failure correction,compensation for varying film and speci­men characteristics, and color temper­ature. Its versatility Is further Increasedby manual override and time exposurecapability to supplement the automaticexposure range. Avallabe In a choice of35mm, 31f.·x41/.- PolaroicP and 4·xS·formats, plus 16mm cine and 35rnm timelapse.The Model PM-10M Manual Photomicro­graphic System permits manual photo­graphy with 35mm and large-formatcameras.

NFK Eyepieces For Photomicropgraphy-A series of eyepieces specifically opti·mized for photography with a microscope.

-Deliver the full optical capabilities of theLa series objectives.

-Available in four magnifications: 2,5X,3.3X, 5X and 6.7X.

Page 19: Olympus Vanox Universal Research Microscope (1983)

"

Standard Outlts

N.B. -When employing objectives with magnifications of 4X or lower, their considerable depth of focusmakes accurate focusing through a normal eyepiece very difficulr This difficulty is solved bycombined use of /he focusing telescope (PM-VTM or PM·VS) or framing eyepiece and /he tocuSIngmagnifier (FT-36).FOf precise tramlllg of the area to be photographed, both of the focusing telescopes have fotmatoutlines n:Jicsting 35mm, 4· x S" and 31/." x 4'1." frame sizes (irl addition to 120 roll film In thePM·VS). Four different framing eyepieces are available tor each frame size.• The color temperarure module PM-eTR, an opllOfJal accessory, simplifies checkrng and adjustmentof microscope illumination color temperature tor optimal results.

,PhotOlTllCl'ography

16nm

"""'" PM-l0 T~'-

"""mICrography

35AD-I L1AO-l L2A0,1

AutomallC Exposure Body ""PBS a a a ;0,

AutornallC Exposure Contr~ Unit ""C8AO a a CI <:JiPower Cold {lor PM.(;BAOI UYCP Il:J Il:J a 0,

Adapte, lor 35mm Camera Back PM-035A. 1:1Adapte, lor Large FQrmal Film Back PM-DL-W a D35mm Camera Back "",C35AO a.. " x 5" ItllenTle()ale AGlapIer f'M..(;4 x S.W II:lI:I'I,"x4"," Polan:'" "". PMCF'·W gFOCU$lng Teoescope """" 0-

Focl.l5mg Magnol>e' PM-FT<36 0Noll. _ tlIlOw

EyepIeCe Adapter lor 'FK" Eyepiece PM'ADF ;0

Color Temperature ModulO ""eTR t)'NolI, __

Tlme Lapse Con1rol UM PM·IV ,0Power Cold (lor PM·IV) UYCP PiPower Coed Ito cornect PM-IV aro uycp IIMICrOSCOPe standl

"'010' 0,,,,,, U"'I lor 16trtn CIne Camera PM-MO g;CIne Adapter PM-DQ'X 9'

45L8D2 a a D tliColor Temperatu,e Compensaloon Filter

45LBT Il:J CI D !2'43ND6·W45 a CI D Q

Neulral Der'lSily Filter43N025·W45 a iCI D ;q.

Page 20: Olympus Vanox Universal Research Microscope (1983)

P...FS........

PM·MDOlympus Malo< cnov. UM

lor 16mm CIne e-I

AH·BISinocul" TulMwith Photo ThlM

PM-CBADAutomlllc ExpoIIn Cont,oI Unlt

AH·NC2Olf'••nlllllntllr1«ll'lolContrat AtuodvnIIll .n......... ""TrIiNIllIttII;IlJoht

250 Film BKk 1

AH·SWTRSUp" WIdIf~1d SlnoculIIThill with P!lOIo TlJbI

AH·PC·LBPhaN ContINI T_e..-

P...PBSAutOlTlll1CExp<ll.U" Bocly

AH·DCW-­...,..-AH·DCD

""­e..-

AH-$C·LB.........e..-

PM-CTRColo< T.-npIIatlQ-.

6JPM·VSFocu11nll TllllCOPI

PM·VTMTu~M..kFoeullngTI!eIeOPI

AH·AAC­_.e..-

~PM.C4x5-W

~ :;,;:;."''-'"

PM·FT·38Focusing Magnltllr

~·x5· FUm "-* lncIucllng PM-CP·WPolaroid" (Commetcl-'Iy_11lIbIeI 3'I,·x~'1.·PolIIrokI" ~

<:::::>

Page 21: Olympus Vanox Universal Research Microscope (1983)

AH·AFL·LH2FII.oof-.oIlMnp HouaIng

AH·AFL-8-LBV..,tc:.llllum....lorIorFI_

""AH·FL·B2,..........1." tOl

AH·AE-",-Q--

AH-MPS-W-"'-

/

/

Page 22: Olympus Vanox Universal Research Microscope (1983)

N''''''-PCSPllOXNH

2OX, dry 0.46 "0 8.03 017 S W , $pfln';Hoadl'ld "''''''PCSPl20XNH

<OX. "0.70 050 413 017 S W, Spriog·oaOOd 4QXPl

PCSPl40XNH

l00X, 011 ,,. 017 >.69 0" S.W. Spr,~OOd, ""'-NH PCSPl l00XNH

A IOXPL

N"<. ""PU 0,. 7,18 "'" 0" PCOA 1oxPU.NH PCOA lOXNHNM PCOA 10XNM

A """"-

"'" ""PU

0'" >'63 .63 011 """"- PCOA "'l<Pt.LNH PCOA 20XNHNM PCOA2OXNMI'l A "''''''-

4O)(dry i'lL 085 060 ". 011 """,,- PCOA 40XPLLNH PCOA 40XNHNM PCOA 40XNM

A l00XPL100)(,011 '''' 0'" '''' 017 Sprlng·k:laded FeDA l00XPLL

~A l00XNHA l00XNM

Page 23: Olympus Vanox Universal Research Microscope (1983)

Microscope Sland

o.Nlluole Revol¥lrt{l Nosepiece

AchromalldA~analicCondenser

centering Frosted Glass

Halogen BUlb (2pcs.}

Eyepece (2pcs,)

D Plan Achromal 4x

D Plan Achromat lOx

D Plan Achromal 20x (spnng)

D Plan Achromat 40x (spnng)

D Plan Achromat l00x (spnng, oj~

S Plan Achromal 4x

S Plan Achromal 10.

S Plan Achroma! 20x (spring)

S Plan Achroma! 40X (spring)

S Plan Achromal 100. (spring. oil)

Module

(1.} (2)

AHB·F·J

UYCP

AH·BI

AH·RE

AH·SV

AH·AAC

AH·SG

AK-LSH·J

JCl2Vl00WHAL

DPl4x

OPllOX

DPl20x

DPL40x

DPL100x

SPL4X

SPllOx

SPL20x

SPl40X

5PLl00x

WHK 10.

Page 24: Olympus Vanox Universal Research Microscope (1983)

An unwavering will to remamat the forefront of sclenlIhc chscovery,and an uncompromisIng conurutment

to quahty have made the nameof Olympus a synonym for high performance

and rellability all over the worldFrom cameras and Inlcrocassette recorders,

to rrucroscopes for various applications,hberscopes, and facsuniles, r.,.,.",.""Olympus has kept abreast

of the most advanced technolOgJesand rnscovenes, constantly stnvrng

to develop products which meet the newand more complex needs U ....;10;1...,IJ,;.1.1.1

of our rapidly changing society.Precision engmeering,

a long experience,and R&D activities tuned to the requirements

of man in his search for a better life,have earned Olympus its unparalleled position

in the medical profession, in the photographic industry,in the laboratory and the classroom,

and in the eyes of all those who benehtfrom the functionallty, accuracy,

versatility and economy of its products.

As .",.~ t_tt• ..Il.y ,oop.....,.. ....J d#'>~/.,.... •• r prod.th. Ill, #'If~~1 • .ppI.,.J _ ... _I q'#'r,. flit 4d.,I. .·.111111, 4U#'rtplt......J/., ,11.01,..,,,,... •11..... I. llu, #'.1&1..,

POOled on J'PI'n M05E.()2838