OIE Reference Laboratory Reports Activities · OIE Reference Laboratory Reports Activities ... Indicated in OIE Manual ... Vet Microbiol 162, 530-542. On-going fitness-

OIE Reference Laboratory Reports ActivitiesOIE Reference Laboratory Reports ActivitiesActivities in 2013Activities in 2013

This report has been submitted : 2014-03-25 15:56:12

Name of disease (ortopic) for which youare a designated OIEReferenceLaboratory:

Highly and low pathogenic avian influenza

Address oflaboratory:

Animal Health and Veterinary Laboratories Agency – Weybridge, Woodham Lane,New Haw,Addlestone, Surrey KT15 3NB, United Kingdom

Tel.: +44-1932 35 73 39

Fax: +44-1932 35 72 39

e-mail address: [email protected]

website: http://www.defra.gov.uk/corporate/vla/science/science-viral-ai-reflab.htm

Name (includingTitle) of Head ofLaboratory(ResponsibleOfficial):

Chris Hadkiss

Name (includingTitle and Position) ofOIE ReferenceExpert:

Ian Brown

Which of thefollowing definesyour laboratory?Check all that apply:

Governmental

OIE Reference Laboratory Reports Activities, 2013 1

Highly and low pathogenic avian influenza - Ian Brown - united kingdom

ToR: To use, promote and disseminate diagnostic methods validated according toOIE Standards

1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as diseasediagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or stafftraining)

Yes

Diagnostic Test Indicated in OIE Manual(Yes/No) Total number of test performed last year

Indirect diagnostic tests Nationally Internationally

HI yes 11874 230

AGP yes 16944 0

ELISA yes 476 0

Direct diagnostic tests Nationally Internationally

Real-time RT-PCR gene M yes 172 151

Real-time RT-PCR H5 yes 4 139

H5 genetic analyses yes 0 10

Real-time RT-PCR N1 yes 0 87

Real-time RT-PCR H7 yes 0 45

ToR: To develop reference material in accordance with OIE requirements, andimplement and promote the application of OIE Standards.To store and distribute to national laboratories biological reference products andany other reagents used in the diagnosis and control of the designated pathogensor disease.

2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE?

No

3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagentsto OIE Member Countries?

Yes

2 OIE Reference Laboratory Reports Activities, 2013


Type of reagentavailable

Relateddiagnostic

testProduced/provide

Amountsupplied

nationally(ml, mg)

Amountsupplied

internationally(ml, mg)

No. ofrecipient

OIEMember

Countries

Region ofrecipients

Control positivesera (OIE-approved

internationalsera)

AGID Produced 237.5ml 0 1

Africa

AmericasAsia and

PacificEuropeMiddle

East

Control negativesera (OIE-approved

internationalsera)

AGID Produced 7 ml 0 1

Africa

AmericasAsia and

PacificEuropeMiddle

East

Control positivesera (OIE-approved

internationalsera)

HIserological

testProduced 5 ml 188 ml 17

Africa

AmericasAsia and

PacificEuropeMiddle

East

AI type antigensHI

serologicaltest

Produced 190 ml 1766 ml 17

Africa

AmericasAsia and

PacificEuropeMiddle

East

Control negativesera (OIE-approved

internationalsera)

HIserological

testProduced 0 5.5 ml 3

Africa

AmericasAsia and

PacificEuropeMiddle

East

Real-time PCRprimers and

probe

H7 (HA2)RRT-PCR(for H7N9detection)

Produced 0 Three vials 1

Africa

AmericasAsia and

PacificEuropeMiddle

East

4. Did your laboratory produce vaccines?

No



5. Did your laboratory supply vaccines to OIE Member Countries?

No

ToR: To develop, standardise and validate, according to OIE Standards, newprocedures for diagnosis and control of the designated pathogens or diseases

6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designatedpathogen or disease?

Yes

7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

No

Name of the newtest or diagnosticmethod or vaccine

developedDescription and References (Publication, website, etc.)

Statisticalassessment of thevalidity of testingLPAI swabs eithersingly or as pools

of five H9 RRT-PCRand molecularepidemiology.

Hard-copy publication available now:: Arnold, M.E., Slomka, M.J., Coward, V.J., Mahmood, S.,Raleigh, P.J., and Brown, I.H. (2013) Evaluation of the pooling of swabs for real-time PCR

detection of low titre shedding of low pathogenicity avian influenza in turkeys. Epidemiologyand Infection. Epidemiology and Infection, 141: 1286-1297. doi: 10.1017/SO950268812001811

Described in: Slomka, M.J., Hanna A., Mahmood, S., Govil, J., Krill, D., Manvell, R.J., Shell, W.,Arnold, M.E., Banks, J. and Brown,I.H. (2013) Phylogenetic and molecular characteristics of

Eurasian H9 avian influenza viruses and their detection by two different H9-specific RealTimereverse transcriptase polymerase chain reaction tests. Vet Microbiol 162, 530-542.

On-going fitness-for-purposechecking of

existing moleculartests on all AIV

strains received atAHVLA

Quantitative and qualitative assessments of the fitness-for-purpose of the assays are made instrict accordance with criteria outlined in the OIE manual and presented at the Joint Nineteenth

Annual Meetings of the National Laboratories for Avian Influenza and Newcastle Disease ofEuropean Union Member States 2013, Helsinki, Finland, 24-26 September 2013

(http://ec.europa.eu/food/animal/diseases/controlmeasures/avian/crls_proceedings_en.htm).Two technical forum presentation titles: 1. H9 AIV: Epidemiology and detection in Eurasia.

Marek J Slomka 2. Evaluation by AHVLA of M-gene (Nagy et al 2010) RRT-PCR….with acomment on H5N1 clade 2.3.2.1 (Indonesia 2013) detection by H5 RRT-PCR. Marek J Slomka

Development ofnew blocking ELISAmethod for testing

for H5 and H7specific antibodies

(Preliminaryvalidation)

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 andH7 antibodies. Jensen, T.H., Ajjouri, G., Handberg, K.J., Slomka, M.J., Coward, V.J., Cherbonnel,M., Jestin, V., Lind, P., and Jørgensen, P.H. (2013). Acta Veterinaria Scandinavica 55: 84. doi:

10.1186/1751-0147-55-84.

Fitness for purposeof EU-

recommended AIRealTime PCRs

(both generic & H7-specific) for

sensitive detectionof ‘new’ H7N9

which emerged inChina during 2013

as a zoonoticthreat.

Information posted on FLU-LAB-NET website, thread entitled “H7N9 influenza A virus inhumans in China” available at: http://forums.flu-lab-net.eu/forums/t/1376.aspx Contributed to

OIE/FAO OFFLU initiative which listed molecular tests which are appropriate for sensitivedetection of the newly-emerged H7N9 virus in China: http://www.offlu.net/index.php?id=267



ToR: To provide diagnostic testing facilities, and, where appropriate, scientific andtechnical advice on disease control measures to OIE Member Countries

8. Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes

Name of OIE Member Countryseeking assistance Date (month)

No. samples receivedfor provision of

diagnostic support

No. samples received forprovision of confirmatory

diagnoses

DENMARK 22/01 0 1

NEPAL 15/02 0 8

NEPAL 05/03 0 3

NEPAL 25/03 0 5

NEPAL 03/04 0 2

THE NETHERLANDS 03/04 0 3

NEPAL 04/04 0 6

MALTA 08/05 6

AUSTRIA 20/06 0 5

DENMARK 20/06 0 1

NEPAL 21/06 0 44

SPAIN 26/06 0 1

SOUTH AFRICA 04/07 0 10

ITALY 17/07 0 5

BOTSWANA 30/07 0 11

ITALY 29/08 0 1

JORDAN 09/10 6

9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes



Name of the OIEMember Country

receiving atechnical

consultancy

Purpose How the advicewas provided

SOUTH AFRICA OIE-funded twinning project with AHVLA-Weybridge Remoteassistance

BOTSWANA OIE-funded twinning project with AHVLA-WeybridgeInspection visit

Remoteassistance

SOUTH AFRICA Provision of disease and technical consultancy in relation to thecontrol of NAI

Remoteassistance

MOLDOVA TAIEX funded EU mission Country visit

MOLDOVA Ad-hoc technical support on AI diagnosis and surveillance Remoteassistance

AUSTRALIA

Ad-hoc technical support on AI diagnosis and surveillance.Queries in 2013 included some noteable emphasis concerning (i)feather testing for H5N1 HPAI diagnosis & (ii) fitness for purpose

of H7 RRT-PCR tests for the sensitive detection of the H7N9which emerged in China in 2013.

Remoteassistance

AUSTRIA



Remoteassistance

BELGIUM



Remoteassistance

CZECH REPUBLIC



Remoteassistance

ToR: To carry out and/or coordinate scientific and technical studies in collaborationwith other laboratories, centres or organisations

10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countriesother than the own?

Yes



Title of the study Duration Purpose of the study Partners (Institutions)

CoVetLab (“Club 5”)Commencedautumn 2012

for 1 year

AHVLA has the lead role in anextension of the below Reference

Laboratory activity to evaluatedifferent algorithms for

serological identification of NAI infarmed galliformes

AHVLA, UK AHVLA(UK) DTU,

Copenhagen,Denmark SVI,

Uppsalla, Sweden CVI,Lelystad, Netherlands

Use of Stable Isotopes toTrace Bird Migrationsand Molecular Nuclear

Techniques toInvestigate the

Epidemiology andEcology of the Highly

Pathogenic AvianInfluenza

2013 - 2016

AHVLA has a lead role inproviding technical consultancy

for AI & PMV-1 RealTime PCRtesting, transportation & storage

of field specimens plussequencing.

University of ForestrySofia Bulgaria

ToR: To collect, process, analyse, publish and disseminate epizootiological datarelevant to the designated pathogens or diseases

11. Did your Laboratory collect epizootiological data relevant to international disease control?

Yes

12. Did your laboratory disseminate epizootiological data that had been processed and analysed?

Yes

13. What method of dissemination of information is most often used by your laboratory?(Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals: 14Peer Review

1. Slomka, M.J., Hanna, A., Mahmood, S., Govil, J., Krill, D., Manvell, R., Shell, W., Arnold, M., Banks, J., and Brown,I.H.(2013): Phylogenetic and molecular characteristics of Eurasian H9 avian influenza viruses and their detectionby two different H9-specific RealTime reverse transcriptase polymerase chain reaction tests. VeterinaryMicrobiology 162 (2013) 530 - 542.http://www.sciencedirect.com/science/article/pii/S0378113512006049http://onlinelibrary.wiley.com/doi/10.1111/j.1750-2659.2012.00397.x/pdf2. Londt, B.Z., Brookes, S.M., Kelly, M.D., Nash, B.N., and Brown, I.H., (2013): Failure to infect pigs co-housed withducks or chickens infected experimentally with A/turkey/Turkey/1/2005 (H5N1) highly pathogenic avian influenzavirus. Veterinary Microbiology 162 (2013) 944–948.http://www.sciencedirect.com/science/article/pii/S03781135120067363. Arnold, M.E., Irvine, R.M., Tearne, O., Rae, D., Cook, A.J.C., and Breed, A.C., (2013) Investigation into samplingstrategies in response to potential outbreaks of Low Pathogenicity Notifiable Avian Influenza initiated incommercial duck holdings in Great Britain. Epidemiology and Infection, Vol 141, (4) 751-762. CambridgeUniversity Press 2013. DOI: http://dx.doi.org/10.1017/S0950268812001483 (About DOI)4. Londt, B.Z., Brookes, S.M., Nash, B.J., Núñez, A., Kelly, M.D., Garcon, F., Graham, S.P., and Brown I.H., (2013) :Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organcultures following adaptation by in vitro passage. Virus Research 178 (2) 383-391.http://dx.doi.org/10.1016/j.virusres.2013.09.0155. Sutton, D., Aldous, E.W., Warren, C.J., Fuller, C.M., Alexander, D.J., and Brown, I.H. (2013). Inactivation of the



infectivity of two highly pathogenic avian influenza viruses and a virulent Newcastle disease virus by ultravioletradiation. Avian Pathology 42 (6) 566-568. http://dx.doi.org/10.1080/03079457.2013.853867http://www.sciencedirect.com/science/article/pii/S016817021200473X6. Long, J.S., Howard, W.A., Núñez, A., Moncorgé, O., Lycett, S., Banks, J., and Barclay, W.S. (2013). The effect ofthe PB2 mutation 627K on highly pathogenic H5N1 avian influenza virus is dependent on the virus lineage. Journalof Virology. doi:10.1128/JVI.01399-13.7. Arnold, M.E., Slomka, M.J., Coward, V.J., Mahmood, S., Raleigh, P.J., and Brown, I.H., (2013): Evaluation of thepooling of swabs for real-time PCR detection of low titre shedding of low pathogenicity avian influenza in turkeys.Epidemiology and Infection. 141 (06) 1286–1297. doi: 10.1017/SO9502688120018118. Iqbal, M., Reddy, K.B., Brookes, S.M., Essen, S.C., Brown, I.H. and McCauley, J.W. (2013) Virus pathotype anddeep sequencing of the HA gene of a low pathogenicity H7N1 avian influenza virus causing mortality in turkeys.Veterinary Research. PLOS ONE. www.plosone.org/.../info%3Adoi%2F10.1371%2Fjournal.pone.00870769. Bahl, J., Besselaar, T., Brown, I.H., Capua, I., Cattoli, G., Chen, H., Cox, N., Claes, F., Dauphin, G., Todd Davis, C.,Donis, R.O., Fouchier, R.A.M., Guan, Y., Hamilton, K., Jang, Y., Kawaoka, Y., Kelso, A., McCauley, J., Mumford, E.,Perdue, M.L., Prajitno, T., Russell, C.A., Smith, D., Smith, G.J.D., Shu, Y., Tashiro, M., Shepard, S., Suarez, D., Viaykrishna, D., Webby, R., Webster, R., and Wong, F. (2013). World Health Organization/World Organisation forAnimal Health/Food and Agriculture Organization (WHO/OIE/FAO) H5N1 Evolution Working Group (2014) Revisedand updated nomenclature for highly pathogenic avian influenza A (H5N1) viruses. Influenza and OtherRespiratory Viruses DOI: 10.1111/irv.1223010. Jansen, C.A., de Geus E., van Haarlem, D.A., van de Haar, P.M., Löndt, B.Z., Graham, S.P., Göbel, T.W., vanEden, W., Brookes, S.M., and Vervelde, L., (2013) Differential lung NK cell responses in avian influenza virusinfected chickens with pathogenicity. Scientific Reports 3: 2478 http://dx.doi.org/10.1038/srep0247811. Irvine, R.M., (2013) Recognising avian notifiable diseases 1. Avian influenza. In Practice Vol 35 (8) p426 – 437.http://dx.doi.org/10.1136/inp.f438012. Irvine, R.M., (2013) Recognising avian notifiable diseases 3. Clinical disease in investigations and differentialdiagnoses in poultry. In Practice Vol 35 (10)597-600. http://dx.doi.org/10.1136/inp.f597813. Alkhamis, M., Perez, A., Batey, N., Howard, W., Baillie, G., Watson, S., Franz, S., Focosi-Snyman, R., Onita, I.,Cioranu, R., Turcitu, M., Kellam, P., Brown, I.H., Breed, A,C., 2013. (2013). Modelling the association of space,time, and host species with variation of the HA, NA, and NS genes of H5N1 highly pathogenic avian influenzaviruses isolated from birds in Romania in 2005–2007. Avian Diseases Vol. 57 (3) pp. 612-621.http://dx.doi.org/10.1637/10494-011713-Reg.114. Jensen, T.H., Ajjouri, G., Handberg, K.J., Slomka, M.J., Coward, V.J., Cherbonnel, M., Jestin, V., Lind, P., andJorgensen P.H., (2013) An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5and H7 antibodies. Acta Veterinaria Scandinavica 2013,55:84. http://www.actavetscand.com/content/55/1/84

b) International conferences: 4Conference Abstract

1. Reid, S.M., AL-Adhadh, B., Essen, S.C., Manvell, R., Focosi-Snyman, R., Errington, J., Irvine, R.M., and Brown,I.H., (2013): Co-circulation of variant infectious bronchitis and H9 low pathogenicity avian influenza viruses incommercial poultry in multiple regions of Iraq. World veterinary Poultry Association Congress, 19 – 23 August,2013, Nantes.2. Nash, B., Núñez, A., Hicks, D., Brookes, S.M., and Brown, I.H., Antigenic and chicken embryonic phenotypedifferences between Korean-like and G1 lineage H9N2 avian influenza viruses. Negative Strand Virus meeting 16– 21 June 2013, Granada, Spain.3. Jansen, C.A., de Geus E., van Haarlem, D.A., van de Haar, P., Löndt, B.Z., Graham, S.P., van Eden, W., Gobel,T.W., Brookes, S.M., Vervelde, L., Diminished lung NK cell responses correlate with enhanced pathogenicityfollowing infection with H5N1 avian influenza viruses. 10th International Veterinary Immunology Symposium, 28thAugust – 1st September 2013, Milan. Italy.4. Watson, S., Howard, H., Baillie, G., Alkhamis, M., Franz, S., Onita, J., Cioranu, R., Turcitu, M., Focosi-Snyman, R.,Choudhury, B., Banks, J., Kellam, P., and Brown, I.H. Evolutionary and genetic characterization of Romanian H5N1HPAI virus. Options for the Control of Influenza V11, Cape Town, South Africa, 5 – 10 September 2013.

c) National conferences: 8Conference Abstract

1. Nash, B., Assessment of influenza pathogenicity using respiratory ex-vivo organ culture. EuroSciCon: 6thAnnual Cell Culture Technology Event: Recent advances, future prospects, London, 7th March 20132. Brown, I.H., Global Activity with Animal Influenza and Challenges for Surveillance. Influenza2013, 17 – 18September 2013, St. Hilda’s College, Oxford. Page 19.3. Ceeraz, V., Cox, W.J., Reid, S.M., Howard, W.A., Puranik, A., Collins, S., Manvell, R., Irvine, R.M., and Brown, I.H.The detection of a low pathogenicity avian influenza virus subtype H9 infection in breeder turkeys in the United



Kingdom. Influenza2013, 17 – 18 September 2013, St. Hilda’s College, Oxford. Page 32.4. Fountain, D., Dunham, S.P., Slomka, M.J., Daly, J., Banks, J., and Brown, I.H. Molecular Pathogenesis of AvianInfluenza Virus in Avian Hosts. Influenza2013, 17 – 18 September 2013, St. Hilda’s College, Oxford. Page 36.5. Hanna, A., Howard, W.A., Núñez, A., Barclay, W.S. and Banks, J. Genetic requirements that support H7N7 HPAIphenotype. Influenza2013, 17 – 18 September 2013, St. Hilda’s College, Oxford. Page 37.6. Howard, W., James, J., Mura, M., Moncorge, O., Shelton, H., and Barclay, W. PB1-F2 modulates polymeraseactivity of Influenza in avian cells but not mammalian cells. Influenza2013, 17 – 18 September 2013, St. Hilda’sCollege, Oxford. Page 39.7. Warren, C.J., Fuller, C.M., Sutton, D.A., Seekings, J.M., Aldous, E.W., Irvine, R.M., and Brown, I.H. Understandingenvironmental factors which impede survival of H7N1 and H5N1 highly pathogenic avian influenza (HPAI) virus.Influenza2013, 17 – 18 September 2013, St. Hilda’s College, Oxford. Page 53.8. Warren, C.J., Fuller, C.M., Sutton, D.A., Seekings, J.M., Aldous, E.W., Irvine, R.M., and Brown, I.H. The survival ofhighly pathogenic avian influenza H5N1 and H7N1 viruses in different environmental matrices. Influenza2013, 17– 18 September 2013, St. Hilda’s College, Oxford. Page 53.

d) Other: (Provide website address or link to appropriate information) 2Letters

1. Fouchier, R.A., García-Sastre, A., Kawaoka, Y., Barclay, W.S., Bouvier, N.M., Brown, I.H., Capua, I., Chen, H.,Compans, R.W., Couch, R.B., Cox, N.J., Doherty, P.C., Donis, R.O., Feldmann, H., Guan, Y., Katz, J.M., Kiselev, O.I.,Klenk, H.D., Kobinger, G.,Liu, J., Liu, X., Lowen, A., Mettenleiter, T.C., Osterhaus, A.D.M.E., Palese, P., Peiris, J.S.M.,Perez, D.R., Richt, J.A., Schultz-Cherry, S., Steel, J., Subbarao, K., Swayne, D.E., Takimoto, T., Tashiro, M.,Taubenberger, J.K., Thomas, P.G., Tripp, R.A., Tumpey, T.M., Webby, R.J., Webster, R.G. (Feb 2013) Transmissionstudies Research resume for avian flu (letter). Science 339 (6119) 520-521.http://www.sciencemag.org/content/339/6119/5202. Fouchier, R.A.M., many authors and Brown, I.H., (2013) ‘H5N1 virus: Transmission studies resume for Avian FluResearch Resumes Nature Vol. 493 (7434) page 609 (31 January 2013) http://dx.doi.org/10.1038/nature11858

ToR: To provide scientific and technical training for personnel from OIE MemberCountriesTo recommend the prescribed and alternative tests or vaccines as OIE Standards

14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE MemberCountries?

Yes

a) Technical visits: 0b) Seminars: 0c) Hands-on training courses: 0d) Internships (>1 month): 0



Type of technical trainingprovided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding

country

a Moldova 6 lab personnel

c Trinidad 2

c Denmark 4

c Georgia 1

c Finland 1

b Albania 2

b Armenia 2

b Azerbaijan 2

b Bosnia & Hercegovina 1

b Bulgaria 3

b Croatia 1

b Georgia 1

b Hungary 1

b Latvia 1

b Montenegro 2

b Serbia 2

b Tajikistan 1

b Macedonia 2

b Turkey 1

b Afghanistan 1

b Bangladesh 1

b Indonesia 2

b Iran 1

b Iraq 1

b Jordan 1

b Kuwait 1

b Malaysia 2

b Myanmar 1

b Oman 1

ToR: To maintain a system of quality assurance, biosafety and biosecurity relevantfor the pathogen and the disease concerned

15. Does your laboratory have a Quality Management System certified according to an International Standard?



Yes

Quality management system adopted

ISO 17025

16. Is your laboratory accredited by an international accreditation body?

Yes

Test for which your laboratory is accredited Accreditation body

Haemagglutination inhibition test UKAS

AGIDT UKAS

Matrix (M)-gene PCR UKAS

H5 real-time PCR UKAS

H5 real-time PCR(HA2) UKAS

H7 real-time PCR (cleavage site) UKAS

H5 real-time PCR(HA2) UKAS

Avian influenza virus nucleotide sequencing UKAS

Neuraminidase inhibition UKAS

Virus isolation in duck/goose eggs (via allantoic cavity) UKAS

Virus isolation in SPF chicken eggs (via allantoic UKAS

IVPI UKAS

17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned?

Yes

(See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests forAquatic Animals 2012, Chapter 1.1.1)

ToR: To organise and participate in scientific meetings on behalf of the OIE

18. Did your laboratory organise scientific meetings on behalf of the OIE?

No



19. Did your laboratory participate in scientific meetings on behalf of the OIE?

Yes

Title of event Date(mm/yy) Location

Role (speaker, presentingposter, short

communications)Title of the work

presented

Twinninginitiatives in Africa March Botswana Speaker/Assessor n/a

OIE GeneralAssembly May Paris Invited

H7N9 emerging threatand global

preparedness

ToR: To establish and maintain a network with other OIE Reference Laboratoriesdesignated for the same pathogen or disease and organise regular inter-laboratoryproficiency testing to ensure comparability of results

20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the samepathogen or disease?

Yes

21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for thesame pathogen or disease by organising or participating in proficiency tests?

Yes

Purpose of theproficiency tests: 1

Role of your Reference Laboratory(organiser/ participant)

No.participants

Participating OIE Ref. Labs/ organising OIE Ref.Lab.

Conventional andmolecular panels

for EU NRLsOrganiser 53

Belgium, Hungary, Finland, Slovakia, Latvia,Lithuania, Slovenia, Republic of Ireland, Estonia,Germany, Romania, Portugal, Czech Republic,

Austria, Denmark, Sweden, Italy, Poland, Cyprus,Luxembourg, Norway, Serbia, Macedonia, France,

Singapore, Montenegro, Egypt, Croatia andBotswana (1 lab from each); UK, Bulgaria,

Greece, The Netherlands, Spain, Israel, Ukraineand Switzerland (2 labs from each); Turkey (3

labs) and South Africa (5 labs)

OFFLU proficiencypanels Participant c50

United States Department of Agriculture,National Veterinary Services Laboratories, Ames,

IA, USA (Organiser)

OFFLU proficiencypanels

The OFFLU AI PCR Proficiency Panelwas done in 2012, with detailed resultsprovided during 2013. AHVLA providedfeedback on its performance & activelycontributed to email dialogue with the

organisers which also included ateleconference to discuss what

information should be distilled fromsuch exercises. This in turn influenced

a discussion on how future panelsshould be presented to participants.

c50United States Department of Agriculture,

National Veterinary Services Laboratories, Ames,IA, USA (Organiser)

1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.



22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientificresearch projects for the diagnosis or control of the pathogen of interest?

No

ToR: To organise inter-laboratory proficiency testing with laboratories other thanOIE Reference Laboratories for the same pathogens and diseases to ensureequivalence of results

23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other thanOIE Reference Laboratories for the same disease?

Yes

Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3

Purpose for inter-laboratory test comparisons1 No. participating laboratoriesRegion(s) of

participating OIEMember Countries

Participation in the IAEA-funded “Wild BirdMigration” project (please see (11) above) hasled to AHVLA organising an AI PCR Proficency

Panel which has been shipped to the IAEAlaboratory at Seibersdorf, Austria. IAEA will

distribute this panel among the participatinginstitutes in order to assess their competency

for generic AI detection by RRT-PCR.

The seven above participatinginstitutes (see (11)) plus the IAEASeibersdorf laboratory, ie total of

eight. NB: Some of theseinstitutions do not necessarily fulfil

the role of an AI NationalReference Laboratory in their

respective countries.

AfricaAmericasAsia and PacificEuropeMiddle East

ToR: To place expert consultants at the disposal of the OIE

24. Did your laboratory place expert consultants at the disposal of the OIE?

Yes

Kind of consultancy Location Subject (facultative)

Provision of comments on OIEStandards 0

Comments to Chapter 1.1.1 OIE Terrestrial Manual Alignmentof the manual with code regarding terminology and

classification using definitions

25. Additional comments regarding your report:

See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:


http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing


www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3

Extra information:

Question 1: 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such asdisease diagnosis, screening of animals for export, surveillance, etc.?Test: H7 genetic analyses, Supplied nationally: 0, Supplied internatinally: 16Egg inoculation/HA Supplied nationally: 705 Supplied internatinally: 43IVPI Supplied nationally: 0 Supplied internatinally: 2


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OIE Reference Laboratory Reports Activities · OIE Reference Laboratory Reports Activities ... Indicated in OIE Manual ... Vet Microbiol 162, 530-542. On-going fitness-