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June

2017

Dr Natalie Bloch (Team Leader)

Dr Ibrahim Diallo, Dr Valentyna Sharandak

PVS Laboratory Mission Report

The Kyrgyz Republic

OIE PVS Pathway Laboratory mission report – The Kyrgyz Republic – September 2017

OIE PVS PATHWAY LABORATORY

REPORT FOR

THE VETERINARY SERVICES OF

KYRGYZ REPUBLIC

(26/06/2017- 08/07/2017)

Dr Natalie Bloch (Team Leader)

Dr Ibrahim Diallo (Technical Expert)

Dr Valentyna Sharandak (Technical Expert)

Disclaimer

This mission has been conducted by an OIE PVS Team authorised by the OIE. However, the views

and the recommendations in this report are not necessarily those of the OIE.

The results of this report remain confidential between the evaluated country and the OIE until such

time as the country agrees to release the report and states the terms of such release.

World Organisation for Animal Health

12, rue de Prony

F-75017 Paris, FRANCE

Kyrgyz Republic OIE PVS Pathway Laboratory Mission – 2017


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Table of Contents

Table of Contents ................................................................................................................. i

Acronyms and Abbreviations ............................................................................................ iii

Acknowledgements ............................................................................................................. v

Executive Summary ............................................................................................................ 7

I Introduction .................................................................................................................. 9

II Conduct of the OIE PVS Pathway Laboratory Mission ............................................ 11

II.1 Method, objectives and scope of the mission ................................................... 11

II.2 Availability of data relevant to the mission ....................................................... 13

II.3 Context of the mission ........................................................................................ 15

II.3.A General organisation of the national Veterinary Laboratory Network (VLN) .... 15

II.3.B OIE PVS Evaluation for veterinary laboratory Critical Competencies .............. 16

II.3.C Proposals of the OIE PVS Follow-Up Mission for the national Veterinary

Laboratory Network (VLN) ............................................................................................ 18

II.4 Organisation of the mission ............................................................................... 19

III Analysis of the Demand for Veterinary Laboratory Analysis ..................................... 19

III.1 Official Programmes for disease control .............................................................. 19

III.2 Routine diagnostic tests and disease investigation ............................................. 20

III.3 Food safety tests at the national level ................................................................... 21

III.4 Opportunities for demand ...................................................................................... 21

III.4.1 Aquatic diseases .......................................................................................... 22

III.4.2 Honeybee diseases ...................................................................................... 22

III.4.3 Avian diseases ................................................................................................. 22

III.4.4 Diseases of other species ................................................................................ 22

III.5 Estimation of total demand of tests and cost of reagents for the two regions .. 23

IV Analysis of the current resources of the veterinary laboratory network and

budgets .............................................................................................................................. 23

IV.1 Physical resources and budget estimation ....................................................... 24

IV.1.1 Buildings .......................................................................................................... 24

IV.1.2 Information technology ................................................................................... 24

IVI.1.3. Vehicles .......................................................................................................... 24

IV.1.4 Equipment ........................................................................................................ 25

IV.2 Reagents and consumables .................................................................................. 25

IV.3 Human Resources .................................................................................................. 26

IV.4 Estimated budgets for the North and the South regions .................................. 26

V. Sustainability of the budget: estimated cost of tests and proposed tariffs .............. 28

V.1 Estimation of costs of tests .................................................................................... 28

V.2 Estimation of tariffs of tests ................................................................................... 29

VI. Reorganisation of the Kyrgyz veterinary laboratory network and estimation of

number of staff required ................................................................................................... 31

VI.1 Central laboratories - RCVDES .............................................................................. 31


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VI.2 Regional laboratories ............................................................................................. 32

VI.3 Estimation of number of staff required by the veterinary laboratory network ... 32

VI.3.1 Central laboratories ......................................................................................... 32

VI.3.2 Regional laboratories....................................................................................... 33

VI.4 Training and on-going education .......................................................................... 33

VII Optimisation of the VLN in the next 5 years ............................................................... 35

VII.1 Equipment and sample collection ........................................................................ 35

VII.2 Establish efficient human resources management ............................................. 35

VII.2.1 Establish private laboratories ........................................................................ 35

VII.2.2 Develop awareness programmes................................................................... 35

VII.3 Technical expertise and Quality Assurance ........................................................ 36

VII.4 Establish relevant data management ................................................................... 37

VII.5 Establish a relevant tariff for all tests ................................................................ 37

VII.6 Further reduction the number of regional laboratories ...................................... 37

Conclusions ....................................................................................................................... 41

Annexes............................................................................................................................. 43

Annex 1: Data Recapitulation table for all laboratories of the national network .... 45

Annex 2: Main events and timeline of the mission ......................................................... 47

Annex 3: Analytical Line Tool (equipment required in the regional laboratories) .. 53

Annex 4: Calculation of reagent cost ............................................................................ 55

Annex 5: Demand Tool .................................................................................................... 57

Annex 6: Calculation Tool .............................................................................................. 61


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Acronyms and Abbreviations

AGID Agarose Gel Immuno Diffusion

Agg Agglutination

AH Animal Health

BBAT Buffered Brucella antigen test

BSL-1, 2, 3 Biological Safety Laboratory Level 1, 2, 3

CC Critical Competency (of the OIE PVS Tool)

CEC Cost Estimation Card (of a CC in the PVS Gap Analysis)

CFT Complement Fixation Test

CIF Cost, Insurance and Freight (Incoterm)

CVO Chief Veterinary Officer

ELISA Enzyme-linked immunosorbent assay

FMD Foot and Mouth Disease

FOB Free On Board (Incoterm)

FS Food Safety

GC Gas Chromatography

GMO Genetically modified organism

IFA Immunofluorescent assay

IT Information Technology

LC-MS Liquid Chromatography Mass Spectrometry

LIMS Laboratory Information Management System

LMPD Livestock and Market Development Project

MAT Microscopic Agglutination Test

NDV Newcastle Disease Virus

NSP Non-structural Protein

OIE World Organisation for Animal Health

PPR Peste des Petits Ruminants

PVS OIE Tool for evaluation of the Performance of Veterinary Services (OIE

PVS Tool)

PCR Polymerase Chain Reaction

qPCR Quantitative Polymerase Chain Reaction

RCVDE Republican Centre for Veterinary Diagnostic and Expertise

RT-PCR Reverse Transcription Polymerase Chain Reaction

SIVPS State Inspectorate for Veterinary and Phytosanitary Security

VLN Veterinary Laboratory Network

VS Veterinary Services


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v

Acknowledgements

The OIE PVS Pathway Laboratory Team would like to thank Dr Zhumakanov Kalysbek, Chief

Veterinary Officer, Dr Kasymbekov Joldoshbek, Animal Health Specialist and OIE focal point,

Dr Toroev Mamarasul, Director of the Bishkek Republican Center for Veterinary Diagnostic

and Expertise (RCVDE), Dr Kydyrjychev Asan, Deputy Director of the Bishkek RCVDE, Dr

Abdumomonov Islam, Director of the Osh Republican Center for Veterinary Diagnostic and

Expertise, Dr Alimbekov Shabdan, Chief Specialist and Ms Aalieva Chynara Talantbekovna

– CVO Assistant for their help in the preparation of the mission and also for their continuous

support and patience during the whole course of the mission.

The OIE PVS Pathway Laboratory Team would like to thank all the people from the Bishkek

and the Osh RCVDEs, and all Veterinary Services they met during the mission, for their

welcoming attitude and their professionalism.


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7

Executive Summary

This OIE PVS Pathway Laboratory Mission is part of the OIE PVS Pathway and follows the

OIE PVS Evaluation (“diagnosis”) and PVS Gap Analysis (“prescription”) as a part of the

complement of tools that the OIE can provide to support compliance (“treatment”) of the

quality of Veterinary Services (VS) with international standards as defined in the OIE

Terrestrial Code.

The objective of the OIE PVS Pathway Laboratory Mission in the Kyrgyz Republic was to

provide decision makers with information to allocate appropriate resources to the Veterinary

Laboratory Network (VLN) and to make strategic decisions to support accurate and timely

diagnosis, as well as efficient laboratory analysis while ensuring the sustainability of the

laboratory network.

The OIE PVS Pathway Laboratory Team met and discussed with various members of the

State Inspectorate for Veterinary and Phytosanitary Security (SIVPS). The OIE PVS Pathway

Laboratory came to the following conclusions:

The number of clinical samples currently submitted to the entire VLN is low and is

mainly made of brucellosis serology using Buffered Brucella Agglutination Test (BBAT),

microscopic examination of slides for detection of bacteria and blood parasites and

Ascoli test for the detection of Bacillus anthracis in hides. Regional laboratories

perform microscopic examinations, BBAT and preliminary identification of bacteria and

parasites. All other tests are performed exclusively in the two Republican Centre for

Veterinary Diagnostic and Expertise (RCVDE) of Bishkek and Osh .

Now that the Kyrgyz Republic has joined the Eurasian Economic Commission (EEC),

testing for food safety (FS) is going to be mandatory for all food stuff (microbiological

tests, hormones, pesticides and antibiotic residues) and there is also a plan to

undertake a very large scale testing of food stuff for genetically modified organisms

(GMO) and meat origin.

No official data was available for planned FS programmes. Therefore, the OIE PVS

Pathway Laboratory Team used a hypothetical number of tests derived from various

discussions with epidemiology unit staff and members of the SIVPS.

Based on the OIE PVS Pathway Laboratory Team evaluation, both central laboratories

have enough capacity in terms of staff and equipment to process all demand, current

and prospective. Furthermore, it is essential to limit the implementation of complex

analyses such as PCR (Polymerase Chain Reaction) , Enzyme Linked Immunosorbent

Assay (ELISA) and food safety testing in the two central laboratories to ensure that

these tests are performed under controlled and uniform conditions.

Currently, The Kyrgyz Republic benefits from a vast VLN comprising 26 laboratories:

the two RCVDEs in Bishkek and Osh and 24 regional veterinary laboratories. The

regional laboratories will be reduced to a total of 17 in the next few months and both

RCDVEs will have the same status. The analysis presented here has therefore been

based on these 19 laboratories, the 2 central and 17 regional laboratories. The report

analyses the budget of the VLN with 2 central and 17 regional laboratories and offers a


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number of options to maximise the efficiency of the VLN. In both central laboratories,

the number of sections needs to be reduced, staff need to be relocated and trained to

be multiskilled e.g. most staff should be able to process FS samples.

After assessing the current demand in the Kyrgyz Republic, the OIE PVS Pathway

Laboratory Team also made some recommendations about increasing the demand

for testing in the domain of aquaculture, honey production and poultry production with

a fee recovery scheme.

The Kyrgyz Republic is characterised by a mountainous terrain, which means that

transportation of samples from the field to the laboratories can be lengthy and may

impact on the turn-around time for testing and reporting of results. Therefore,

laboratories must be located strategically to allow a good coverage of the whole

territory. The OIE PVS Pathway Laboratory Team estimates that the number of

regional laboratories can be reduced and still achieve a good coverage of the whole

country in a cost-effective manner.

Consideration should be given to the implementation of private laboratories to perform

tests on clinical cases on a fee for service basis. They will not play any role in the

national programmes which will be the responsibility of the central and regional

laboratories. However, they may play a role in the collection and transport of samples

for further testing. These laboratories could be staffed with former laboratory staff

willing to set up such ventures.

On a more general note, the OIE PVS Pathway Laboratory recommends the following:

The strengthening, and where applicable the finalisation, of a quality management

system across the VLN.

The establishment of relevant data management which will assist in more effective

overall laboratory management, including the analysis of laboratory analysis demand,

the inventory of equipment, the management of human resources and laboratory

analysis related costs.

The establishment of national tariffs for laboratory analysis, using international

reference tariffs as a benchmark, in order to maintain an adequate supply of reagents

and consumables.


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I Introduction

Following a request to the OIE from the Government of the Kyrgyz Republic, an OIE PVS

Pathway Laboratory Mission was conducted from 26th June to 8th July 2017 by a team of OIE

Experts. This mission was based on the national priorities of the Veterinary Services as

defined in the 2008 OIE PVS Gap Analysis Report and the 2016 OIE PVS Evaluation Follow-

Up mission.

According to the annual reports submitted to the OIE, the following diseases were reported

from 2014 to 2017: rabies, brucellosis (B. abortus and melitensis), anthrax, Echinococus

granulosus and multilocularis, Dourine, Foot and Mouth Disease, Sheep and Goat Pox.

The laboratory Critical Competencies pillar of the 2016 OIE PVS Evaluation Follow-up

Mission recommended as a priority to reorganise sampling methods, kits and transportation

to laboratories; to develop skills of veterinarians for clinical diagnosis and submission of

samples to laboratories for confirmation, develop a laboratory information system (LIMS) and

suggested that food safety should be considered as a main investment for the next decade.

The recent report (2017) on the second stage of the Livestock and Market Development

Project (LMDP) identified Echinococcosis and Brucellosis as priority diseases: and it

provided assistance to prepare the Strategic Plan for Foot and Mouth Disease (FMD) and

Peste des Petits Ruminants (PPR).

These reports do not provide enough detail in the analysis of the National Veterinary Laboratory Network budget.

Given the variety of issues to be addressed, the country requested a specific OIE PVS

Pathway Laboratory Mission. The OIE PVS Pathway Laboratory Mission, for which the

methodology, strategies and recommendations are outlined in this report, builds upon the

2016 OIE PVS Follow-up report, recognizing that laboratories are an integral component of

the Veterinary Services and their priority should meet the needs of the Veterinary Services.


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II Conduct of the OIE PVS Pathway Laboratory Mission

II.1 Method, objectives and scope of the mission

This OIE PVS Pathway Laboratory Mission is part of the OIE PVS Pathway and follows the

OIE PVS Evaluation (“diagnosis”) and PVS Gap Analysis (“prescription”), as a part of the

complement of tools that the OIE can provide to support compliance (“treatment”) of the

quality of VS with international standards as defined in the OIE Terrestrial Code.

Objective:

The purpose of many laboratory-related expert technical evaluation missions is to assess or

evaluate technical capacity and suggest improvements. As these types of missions are

generally constrained by the context and terms of reference, they often do not allow for the

in-depth examination of substantive systemic or strategic issues. The most frequent outcome

of these kinds of missions is at best partial examination of structures and systems, to the

detriment of an overarching or strategic view. Often this leads simply to technical

recommendations and over-investment in structures that are systematically unable to

implement them properly.

The overall objective of the OIE PVS Pathway Laboratory Mission is to provide the decision

makers of Veterinary Services (VS) with information to allocate appropriate resources to the

National Veterinary Laboratory system and to make strategic decisions to support accurate

and timely diagnosis, while ensuring the sustainability of the laboratory system.

Method:

Based on established procedures and practices of the OIE PVS Pathway, an OIE PVS

Pathway Laboratory Mission was undertaken in the Kyrgyz Republic to:

Analyse the demand for veterinary laboratory analysis, mainly through current

laboratory activities, the OIE PVS Gap Analysis and described national

programmes, as well as other partners and/or from prospective demand, including

an overall estimation of the number of samples and tests, of the geographical

distribution of demand, of the cost of consumables (including sampling kits, samples

transport and reagents) and of the international cost;

Analyse the existing or potential service delivery or “supply”, laboratory veterinary

analysis at the national level, mainly delivered by the national veterinary laboratory

infrastructures, as well as by other laboratories (from other public institutions or the

private sector), including their existing capacity and their potential access to new

markets to make the laboratory function a more coherent investment; and

Present a range of sustainable management, organizational, budgetary and

financial solutions, with different options and their costs, in order to assist decision

making at the highest levels related to the cost of autonomy and distribution of the

national laboratory network, including options for externalization through official

delegation to the private sector or to international Reference Laboratories.


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Outcomes:

The Veterinary Services decision makers of the Kyrgyz Republic should have a better

understanding of:

the laboratory analysis demand in their country (current and prospective);

sustainable ways to organize the national VLN to make the laboratory function a more

coherent investment; and

how to better allocate or advocate for sufficient resources to support accurate and

timely diagnosis of priority animal diseases and veterinary public health issues.

Outputs:

The present report presents the main outputs of the OIE PVS Pathway Laboratory Mission,

which include:

An estimate of the demand for laboratory tests for the next five years, inclusive of the

related costs of consumables

An estimate of the current delivery, or “supply”, of laboratory analysis, including

prospects for potential new markets;

Proposals of sustainable management and organizational solutions of the national

veterinary network; and

Estimates of human, physical and financial resources needed for the implementation of

the best solutions adapted to the veterinary laboratory network.

Tools:

In order to facilitate the analysis of current and prospective data, the OIE PVS Pathway

Laboratory Mission used several tools, from which are extracted the tables presented below

and in annex. Here is a brief description of the tools and the way they were used during the

mission.

1. Demand Tool

The Laboratory Analysis Demand Tool (the first tool used during an OIE PVS Pathway

Laboratory Mission) validates the estimation made during the OIE PVS Gap Analysis Mission

of the budget required for laboratory analyses for consumables in the national laboratory

network (including cost of reagents, sample kits and local shipment). During this portion of

the mission, the current demand, the OIE PVS Gap Analysis demand, the possible

prospective demand for veterinary laboratory analyses as well as the limiting factors and

opportunities for demand are examined with the assistance of the country team.

2. Supply Tool

The purpose of this tool is to mainly collect data of the full array of veterinary laboratory

analyses offered by the laboratory network. This tool establishes automatic ratios for human,

physical and financial resources. It assesses if the laboratory analyses offered by the

laboratory function meet the demand. In the Kyrgyz Republic all data had to be collected

during the mission and the supply tool could not be used.


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3. Calculation Tool

The Calculation Tool is used to simulate different scenarios depending on the distribution of

the budget. It highlights real costs, possible benefits, or required subsidies.

The tables of the first spreadsheet are incorporated directly into Chapter IV of this report.

The second spreadsheet allows the estimation of the “true costs” of laboratory analyses by

taking into account their respective numbers, and the costs of capital, staff and functioning of

the laboratory.

The third spreadsheet allows an estimation and calculation of a national tariff of laboratory

analyses.

4. Analytical Line Tool

The Analytical Line Tool proposes a pre-established check-list of equipment lines, including a

set of equipment and proposed international prices.

II.2 Availability of data relevant to the mission

The Supply Tool was sent to the nominated country authorities for distribution to all

laboratories, to be completed before the mission and returned to the OIE PVS Pathway

Laboratory Mission. The tool was not disseminated so information had to be gathered during

the first week of the mission.

All the laboratory system’s records are kept on monthly paper based records, and sent

quarterly to the Republican Centre for Veterinary Diagnostic and Expertise (RCVDE) by

email.

Data concerning staff, building, equipment and test numbers were extracted from records

provided by the RCVDE in Bishkek. Except for the RCVDE in Bishkek and the RCVDE in

Osh, the value of existing equipment could not be assessed and the OIE PVS team was told

that most of the equipment was obsolete and would be replaced. Given that no data was

provided to the OIE PVS Pathway Laboratory Team before the mission, it was impossible to

use the OIE supply tool, therefore all the required information was entered in a general data

recapitulation table generated by the OIE PVS Pathway Laboratory Team as shown in Annex

1.

The estimated number of animals in the country (Table 1) was provided by the Deputy

Director of the Bishkek RCVDE. Numbers were rounded.

Table 1: Animal Census Estimate (June 2016) from the Statistical Committee of the

Kyrgyz Republic

Cattle Sheep/goats Horses Rabbits Pigs Poultry

TOTAL 1 500 000 6 000 000 467 000 26 000 51 000 5 7 00 000


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The present organizational chart for the State Inspectorate for Veterinary and Phytosanitary

Security (SIVPSS) was supplied during the mission (Figure 1).

Figure 1: Organisational structure of the SIVPSS


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II.3 Context of the mission

II.3.A General organisation of the national Veterinary Laboratory Network (VLN)

The national VLN of the Kyrgyz Republic has been established as a part of the State Inspectorate for Veterinary and Phytosanitary Security (SIVPSS).

The present VLN of the Kyrgyz Republic consists of two central laboratories, the Bishkek Republican Center for Veterinary Diagnostics and Expertise (RCVDE) and the Osh RCVDE, 5 zonal veterinary laboratories and 19 district diagnostic and inter-district laboratories as well as 4 branches laboratories under the control of the district laboratories (see figure 2 below).

Figure 2: Map of the Kyrgyz Republic with the location of the existing laboratories

The 2 Central laboratories are tagged in red

The Zonal laboratories are tagged in purple

The district and inter-district laboratories are tagged in Pink

The branch laboratories are tagged in Yellow

Based on the recommendation of the OIE PVS Gap Analysis Report and the OIE PVS Evaluation Follow-Up Mission, the Kyrgyz State Inspectorate for Veterinary and Phytosanitary Security have reduced the number of laboratories from 26 to 19. This will be implemented in the next 12 months. The reorganisation of the Veterinary Laboratory Network has been approved by the Government of the Kyrgyz Republic Decree № 346 as of 07 June 2017.

There won’t be a distinction any longer between zonal, district, interdistrict and branch laboratories.

Furthermore, for the purpose of the VLN organisation the country has been divided in 2 zones, the North and South regions. There are 9 laboratories in the North region (8 regional laboratories and the Bishkek RCVDE) and 10 laboratories in the South region (9 regional laboratories and the Osh RCVDE). All the regional laboratories located in the


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North region report directly to the RCVDE in Bishkek while all the regional laboratories located in the South region report to the Osh RCVDE. As part of this reorganisation, a new laboratory will be built and equipped in Balykchy in the North. The Talas and Tokmok laboratories in the North and the Batken laboratory in the South will be completely refurbished and equipped, and 7 district and inter-district laboratories will be closed as well as all four branch laboratories. It is worth noting that some of the remaining laboratories are still less than 100 km apart both in the North and the South.

II.3.B OIE PVS Evaluation for veterinary laboratory Critical Competencies

In Table 2, the level of advancement identified by the Veterinary Services through the

2016 OIE PVS Follow-Up mission are indicated in yellow.

Table 2: Critical Competencies (CC) II-1.A & B, II-2 and II-10

II-1 Veterinary laboratory diagnosis

A. Access to veterinary laboratory diagnosis

The authority and capability of the VS to have access to laboratory diagnosis in order to identify and record pathogenic agents, including those relevant for public health, that can adversely affect animals and animal products.

Levels of advancement

PVS 2007 II-1

1

Disease Diagnosis is almost always conducted by clinical means only, with laboratory diagnosis capability hampered by outdated laboratory equipment and techniques.

Gap target 2008 II-1

3

For diseases of zoonotic or economic importance not present in the country, but known to exist in the region or which could enter the country, the VS can collect samples and ship them to a laboratory which results in a correct diagnosis.

PVS 2016

1 Disease diagnosis is almost always conducted by clinical means only, with no access to and use of a laboratory to obtain a correct diagnosis.

2 For major zoonoses and diseases of national economic importance, the VS have access to and use a laboratory to obtain a correct diagnosis.

3 For other zoonoses and diseases present in the country, the VS have access to and use a laboratory to obtain a correct diagnosis.

4

For diseases of zoonotic or economic importance not present in the country, but known to exist in the region and/ or that could enter the country, the VS have access to and use a laboratory to obtain a correct diagnosis.

5

In the case of new and emerging diseases in the region or world, the VS have access to and use a network of national or international reference laboratories (e.g. an OIE Reference Laboratory) to obtain a correct diagnosis.

Although, the OIE PVS has identified some strengths, some weaknesses were also identified:

Lack of organization to ease shipping of samples;

Old methods for sampling kits and transportation with reusable glass tubes, imposing a cost of transportation and manpower which cannot fit with a national programme;

Lack of initiative of private veterinarians to initiate laboratory diagnosis


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II-1 Veterinary laboratory diagnosis

B. Suitability of national laboratory infrastructures

The sustainability, effectiveness and efficiency of the national (public and private) laboratory infrastructures to service the needs of the VS.


PVS 2007

-

Gap target 2008

-

PVS 2016

1 The national laboratory infrastructure does not meet the need of the VS.

2

The national laboratory infrastructure meets partially the needs of the VS, but is not entirely sustainable, as organisational deficiencies with regard to the effective and efficient management of resources and infrastructure (including maintenance) are apparent

3

The national laboratory infrastructure generally meets the needs of the VS. Resources and organisation appear to be managed effectively and efficiently, but their regular funding is inadequate to support a sustainable and regularly maintained infrastructure

4

The national laboratory infrastructure generally meets the needs of the VS and is subject to timely maintenance programmes but needs new investments in certain aspects (e.g. accessibility to laboratories, number or type of analyses).

5 The national laboratory infrastructure meets the needs of the VS, and is sustainable and regularly audited.

II-2 Laboratory quality assurance

The quality of laboratories (that conduct diagnostic testing or analysis for chemical residues, antimicrobial residues, toxins, or tests for, biological efficacy, etc.) as measured by the use of formal QA systems including, but not limited to, participation in relevant proficiency testing programmes.


PVS 2007

-

Gap target 2008

2 Some laboratories used by the public sector VS are using formal QA systems.

PVS 2016

1 No laboratories used by the public sector VS are using formal QA systems.

2 Some laboratories used by the public sector VS are using formal QA systems.

3 All laboratories used by the public sector VS are using formal QA systems.

4 All the laboratories used by the public sector VS and most or all private laboratories are using formal QA systems.

5

All the laboratories used by the public sector VS and most or all private laboratories are using formal QA programmes that meet OIE, ISO 17025, or equivalent QA standard guidelines.


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II-10 Residue testing

The capability of the VS to undertake residue testing programmes for veterinary medicines (e.g. antimicrobials and hormones), chemicals, pesticides, radionuclides, metals, etc.


PVS 2007

-

Gap target 2008

3 A comprehensive residue testing programme is performed for all animal products for export and some for domestic consumption.

PVS 2016

1 No residue testing programme for animal products exists in the country.

2 Some residue testing programme is performed but only for selected animal products for export.

3 A comprehensive residue testing programme is performed for all animal products for export and some for domestic consumption.

4 A comprehensive residue testing programme is performed for all animal products for export and domestic consumption.

5 The residue testing programme is subject to routine quality assurance and regular evaluation.

II.3.C Proposals of the OIE PVS Follow-Up Mission for the national Veterinary Laboratory Network (VLN)

The proposed strategy with regards to the laboratory services was to:

Reorganize sampling methods, transportation and use modern kits and labelling methods of samples for national programmes;

Develop skills of field veterinarians for clinical diagnosis, for necropsy and submission of samples to laboratories for confirmation

Reorganize the laboratory network, possibly with a support of an OIE PVS Pathway Laboratory Mission in order to determine a strategy for sustainable and effective laboratory network;

Ensure capacity of anatomic-pathology and biochemistry in central veterinary laboratories

Implement a computerised laboratory information system and improve the registration of samples in the laboratories using spreadsheet software (e.g. Excel) allowing the analysis of data.


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II.4 Organisation of the mission

During the mission, the OIE PVS Pathway Laboratory Team visited the RCVDE in Bishkek,

the RCVDE in Osh and the Chuiskiy Interdistrict Center for Veterinary Diagnostic and

Expertise

The main events and timeline of the mission are described in Annex 2.

III Analysis of the Demand for Veterinary Laboratory Analysis

As the new organisation of the VLN is taking effect immediately and will be implemented

within the next 12 months, the following analysis and calculations have been made taking

into account the new structure.

III.1 Official Programmes for disease control

The official surveillance programme will involve the analysis of samples for 12 diseases.

Discussion with laboratory staff and the epidemiology department of the State Inspectorate

on Veterinary and Phytosanitary Security under the Government of the Kyrgyz Republic have

indicated that the geographical distribution of animals in the country is such that 65% of all

official programme testing will be performed in Osh region and 35% in Bishkek region with

the exception of Leucosis for which 65% of testing will be done in Bishkek (because of the

high number of breeding and dairy cattle in the region).Table 3 shows the number of samples

required for the official programmes.

Table 3: Official programmes

Disease and technique Total Number Bishkek region Osh region

1. BRUCELLOSIS

Brucellosis by BBAT 1 200 000 420 000

780 000

Brucellosis by CFT

(Complement Fixation Test) (10%

of BBAT)

120 000 42 000 78 000

2. PPR ELISA 60 000 21 000 39 000

3. Echinococcosis

(identification at

the family level –

Taenidae)

20 000 7000 13 000

4. FMD

FMD NSP (Non structural

Protein) ELISA

12 000 4 200 7 800

FMD SP (Structural Protein )

ELISA

8 000 2 800 5 200

5. Anthrax (soil

examination)

1000 350 650

6. Leucosis by AGID 50 000 32 500 17 500


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(Agar Gel Immuno

Diffusion)

7. Sheep and Goat

Pox by AGID

30 000 10 500 19 500

8. Glanders by CFT 28 200 9870 18330

9. Dourine by CFT 28 200 9870 18330

10. Leptospirosis by

MAT (MicroAgglutination

Test) (8 serovars)

40 000 14 000 26 000

11. Avian Influenza by

ELISA

2000 700 1400

12. Rabies by IFA

(immunofluorescence

Assay)

500 175 325

The cost of reagents for these official programmes only is approximately € 185,000 for the

North region and €337,000 for the South. The cost of reagents for these programmes will

have to be budgeted for by the government.

III.2 Routine diagnostic tests and disease investigation

The number of tests performed in each laboratory for clinical samples, as shown in the

Recapitulation table in Annex 1, was supplied by the Bishkek RCVDE. However the data

provided were not consistent as a number of regional laboratories were shown to perform

Ascoli, and/or rabies testing, while discussion with the Chief Veterinary Officer confirmed that

regional laboratories do not perform such tests. Further inconsistencies of number of

performed tests were noted. For instance, since the 1st January 2017 the Bishkek RCVDE

registry had only 776 entries, some of which could be for multiple samples and analyses. The

number of parasitological tests also seemed very high with values of 100,000 tests for the

country whereas examination of registries showed a much lower number of samples.

Overall, the bulk of tests performed are faecal and blood parasitological examinations,

staining and microscopic examination of slides for bacteria, ring test on milk and antrax

testing of hides using Ascoli test. Discussions with the epidemiology team indicated that an

estimated number of 250,000 Ascoli tests will be performed on hides for the whole country.

The number of ring tests performed in 2016 was 25,000 for the whole country. It is estimated

that this number will not change significantly over the next years.

There are currently approximately 5000 tests performed for bee diseases and this number

will probably not change.

The number of other diagnostic tests such as Aujezski, Equine Arthritis, Gumboro, Newcastle

disease, egg drop syndrome etc. is very low and it is estimated that this number will not

change significantly over the next years.

The estimation of the number of tests for the coming years is shown in the Demand tool in

Annex 5.


21

All these tests should be performed at customers’ expense and official tariffs should be used

as recommended in chapter V.2

III.3 Food safety tests at the national level

Since 2014 Kyrgyz Republic has been part of the Eurasian Economic Commission (EEC)

which requests from its members a strict control on food stuff. The Kyrgyz Republic is also

preparing a reform of the legislation concerning food safety (FS), which will make testing

compulsory for all exporters of food stuffs as well as all local manufacturers, at the expense

of the businesses. FS testing is currently limited to antibiotic detection in exported food stuffs

using colorimetric detection. The OIE PVS Pathway Laboratory Team was informed that 51

establishments preparing food staff have been approved for the export of food stuff to

member states of the EEC .Therefore member states have to abide to the technical

regulation of the Custom Union ТР ТС 034/2013 on milk and meat products. Testing must be

done for the followings:

Hormones

Heavy Metals (Arsenic, Mercury, Cadmium, Lead etc.)

Pesticides (an average of 6 parameters)

Radiological residues (Cesium and Strontium)

Aflatoxin

It is also anticipated that both central laboratories will be able to provide testing for the

identification of the origin of meat by genotyping and the detection of Genetically Modified

Organisms in food stuffs.

The proposed planning consists in taking 10 samples from each batch of food stuff. Based

on a production of 5 batches per month and per producer, the total yearly sample number for

each producer will be 600 and therefore 30,600 for the whole country for hormone, heavy

metals and pesticides. However no number has been suggested for GMP and genotyping for

meat origin.

Since the repartition of the 51 establishments could not be ascertained, calculations were

made on the basis of equal distribution of the tests between the 2 central laboratories.

This mandatory test will have to be performed at clients’ expenses and the government might

choose to partially subsidize the cost of the tests.

III.4 Opportunities for demand

Aquatic animal diseases, honey bee diseases as well as certain avian and equine diseases

are worth servicing. Most of the diseases of interest can be diagnosed using conventional

methods e.g. microscopy, floatation, bacterial isolation and identification. However, the use

of polymerase chain reaction either conventional or real-time will also ensure an improved

diagnosis of a variety of diseases, and a better diagnostic turn-around time. The 2 central

laboratories are already equipped with real-time PCR machines which are supposed to be

used for food safety testing. It would be more cost efficient to also use real-time PCR for the

diagnosis of some animal diseases. The OIE PVS Pathway Laboratory Team came to the

conclusion that the adaptation of PCR techniques would greatly improve diagnostic outcome


22

at the central level, while the use of the above mentioned conventional techniques would

achieve not only a better diagnostic servicing of common diseases in Kyrgyz Republic, but

also an efficient use of staff at the regional level.

III.4.1 Aquatic diseases

In a mission commissioned by the Ministry of Agriculture and Water Resources and

Production Integrity (MAWRPI) in 2008 ,it was noted that the fisheries production has

declined from more than 1,361 Tons in 1991 to 71 tons in 2006 since the independence in

1991 (Sarieva et al. 2008). The will to develop fisheries production in a bid to alleviate

poverty and ensure food security can be supported by the provision of laboratory diagnostic

services to the fisheries. Some of the most common diseases that affect fish, such as

parasitic and bacterial diseases can be investigated in a bid to help improve the quality of

fish sold in Issyk-Kul lake region and other parts of the country. This seems to be currently

lacking and present an opportunity for developing some fee-for-service agreement with the

MAWRPI.

III.4.2 Honeybee diseases

Although there are national programmes on Varroasis and both the European and American

foulbroods, there is a niche for more fee-for-service testing of local honey production. This

production is already subjected to testing due to food safety requirements. Therefore, it will

be very fitting to accompany that with a sound testing regime of honeybees for the

abovementioned diseases under a fee-for-service regime devised by the SIVPS. More

importantly, the diagnosis of honeybee diseases does not require expensive equipment and

could be performed in all 17 laboratories if required. Such tests consist of microscopy and

mite identification. Other diseases relevant to the context of the Kyrgyz Republic might

considered once the routine diagnosis of varroasis and the 2 common foulbroods have been

implemented.

III.4.3 Avian diseases

According to discussions held with SIVPS staff, there are a number of chicken farms

especially in the Chuy region and also the Talas region. These farms may have private

laboratories that perform basic bacteriological and serological testing of the farm flocks. The

farms will benefit from accessing the expertise of the 2 central laboratories in bacteriology

and virology. The service provided by the two central laboratories would be based on a fee-

for-service, and the farms wouldl send carcasses of dead birds to the central laboratories for

full investigations of the causes of mortality.

III.4.4 Diseases of other species

Currently the Veterinary Research Institute is conducting some research on equine

respiratory viruses such as equine herpesvirus, an important pathogen of horses. It would be

fitting to transfer the technology to the central laboratories once the methodology has been

validated.

Similarly, the diagnosis of echinococcosis is only done to the family level (Taenidae) and no

speciation of the parasite is done. To improve the diagnosis of such an important zoonotic

disease, the Veterinary Research Institute could adopt a published real-time PCR method for

the detection and differentiation of E. multilocularis and E. granulosis and transfer the


23

validated method to the central laboratories.

The Ministry of Agriculture has indicated that they will require to perform some tests to detect

pesticide residues in feed. These tests could be performed by the central laboratories and

charged to the end user.

A number of small private laboratories exist in dairy and chicken farms. The central

laboratory could play a role in offering some internal accreditation scheme and confirmation

tests.

III.5 Estimation of total demand of tests and cost of reagents for the two regions

The total cost of analysis does not include the cost of maintaining laboratory

equipment/infrastructure or the salaries of staff required to perform these tests.

Biochemistry and haematology tests as well as feed content analysis are not taken into

account in the prospective demand tool.

The prospective demand tables for all tests and both region are shown in Annex 5.

Both central laboratories will perform tests for the official surveillance programmes and food

safety (FS).

The regional laboratories will perform the following analysis:

Parasitological examinations, either for clinical samples or for official programmes.

This includes coprology for gastrointestinal parasites e.g. helminths and microscopy

of blood smears for blood parasites e.g. theileria, anaplasma, babesia etc.

Serological tests: Buffered Brucella antigen test (BBAT) and Complement Fixation

test (CFT) as a confirmation test for the detection of Brucellosis in breeding cattle,

male non-vaccinated small ruminants, horses and dogs

Bacteriological examination for clinical samples including staining of slides and

microscopy, initial bacterial culture and gram stain. It is assumed that the regional

laboratories will forward any bacterial isolates to the central laboratory of their region

for further identification.

In the North region, the total number of tests including official programmes is estimated at

approximately 960 000 tests and the cost of reagents at €762 000 (€ 185 000 for the official

programmes only)

In the South region the total number of tests including official programme is estimated at

approximately 1.4 Million tests and the cost of reagents at €932 000 (€337 000 for the official

programmes only).

IV Analysis of the current resources of the veterinary laboratory network and budgets

Considering the new legislation enforcing the existence of two Central Laboratories at the

same level and with independent budgets the analysis has been made for two entities. Since

the number of laboratories will be reduced in the next few months, all calculations were done


24

for the infrastructure of the 2 Central Laboratories and 17 regional laboratories. However, the

total number of staff presently working in the 26 laboratories has been taken into account

since this number will not be reduced in the near future as staff from laboratories to be

closed are due to all be relocated in remaining laboratories.

All data are shown in the recapitulation table in Annex 1.

IV.1 Physical resources and budget estimation

IV.1.1 Buildings

The cost of construction of a building, equivalent to a Biological Safety Laboratory Level 1

(BSL1), is estimated at € 730/m2 in Bishkek and € 350/m2 in regional areas in the North of

the country. In the South, the price is estimated at € 530/m2 in Osh and € 150/m2 in regional

areas.

The cost of construction for a Biological Safety Laboratory Level 2 (BSL-2) according to

international standards (including waste management and air filtering and pressure systems)

is estimated to be double the price of BSL-1. Hence, the calculated cost of €1,500/m2 in

Bishkek for BSL2 and €1,100/m2 in Osh.

OIE estimates the cost of BSL3 to be approximately € 5,000 per square meter (including

waste management, air filtration and pressure systems), regardless of the country.

Maintenance of current buildings is estimated to be five percent of construction costs per

year.

It was estimated that the surface of the new laboratory surface of Balykchy will be

approximately 300 m2.

Data supplied by the Bishkek RCDVE for the surface of each laboratory are shown in the

recapitulation table in Annex I. The surfaces for a number of laboratories could not be

verified but seemed at odd with the other values, therefore surface values were

approximated.

The value of buildings for the 9 laboratories of the North region was estimated at

approximately € 3 Million (see recapitulation table in Annex 1) and the maintenance budget is

estimated at € 156,000. It is noteworthy that the Bishkek RCVDE is planning to upgrade the

250 m2 surface of the virology laboratory into BSL3. If this was the case the total value of the

building would be increased to €4.25 Million and the budget of maintenance would be

€220,000.

The value of buildings for the 10 laboratories of the South region was estimated at

approximately € 783 000 and the maintenance budget is estimated at € 39 000.

IV.1.2 Information technology

All the laboratories have one telephone line and internet connection and computers.

Computers and other office or telecommunication equipment renewal should occur every

three years (33% per year). Local costs are applied.

IVI.1.3. Vehicles

The value of a car has been estimated at €10,000. Renewal of vehicles should occur every

five years (20% per year).


25

IV.1.4 Equipment

The majority of equipment is manufactured in the Russian federation except for the new

Japanese and European equipment for Food Safety analysis recently acquired as part of a

project funded by The Russian Federation and the Republic of Kazakhstan.

A list of equipment in the 26 existing laboratories was supplied during the mission, but the

number of equipment and their total value in all the regional laboratories was inconsistent

with the number of modern equipment required to perform basic bacteriology, parasitology

and serology testing. The existing equipment has no residual value and cannot, in many

instances, be serviced or repaired if they are damaged. Furthermore, the equipment

available in the regional laboratories is obsolete and cannot be reliable to perform to the

standards required for any accredited veterinary laboratory.

For the purpose of budget calculation, international equipment prices were used for the 17

regional laboratories. The value of required equipment was estimated at € 36,600 per

laboratory as shown in Annex 3.

The value of the equipment in the two central laboratories (€3,3 Million for the Bishkek

RCVDE and €1 352,000 for Osh RCDVE was supplied to the OIE Pathway Laboratory Team

by the RCVDE and is based on invoices of the recently acquired equipment.

As a reference and to maintain quality assurance, it is estimated that:

Renewal of laboratory equipment should occur on average every ten years (10% per year)

Calibration and metrology costs are estimated at approximately 10% of the equipment

value per year.

During visits of the Bishkek and Osh RCVDEs the OIE PVS Pathway Laboratory Team

established that both laboratories conducted corrective and preventive maintenance as well

as metrology and calibration programmes. As for other laboratories around the country,

these were reported to be carried out and were mostly coordinated by the Bishkek RCVDE.

IV.2 Reagents and consumables

Sample collection for serum is still not performed with vacutainers but with reusable and

washable glass tubes estimated at 7.5 Som each, which is equivalent to €0,1. Therefore the

cost of sample collection has been adjusted as shown in all the demand tables in Annex 2.

The parametric cost of local transport from the field to the laboratories for 25 samples is

estimated at €11.

The OIE PVS Pathway Laboratory Team was provided with an extensive list of invoices for

various reagents and consumables which allowed the exact calculation of reagent cost

(Annex 4). The data provided were used in the demand table. Because a lot of reagents are

prepared locally, the costs are often at odd with values in other countries.

For example, the cost of MAT consists only of the cost of the Leptospira growth medium,

1 However the Bishkek RCVDE laboratory spends €350 per year to send samples overseas for proficiency testing

while the Osh RCVDE spends €1,000 per year to send samples to Russia. (source of this information is field

interviews)


26

which contains rabbit hyperimmune serum. The cost of maintaining rabbits in the vivarium

was not included, mainly because the estimated costs provided were not realistic e.g. €0,013

to maintain 1 rabbit.

When no invoices were provided, OIE estimation was used, for example for PCR or ELISA

tests.

IV.3 Human Resources

As mentioned above, out of the 26 laboratories throughout the country, 7 will be closed. It is

anticipated that all staff from these 7 laboratories will be relocated in the other 17 remaining

laboratories.

Annex 1 shows that in the North region there are at present a total of 198 staff (including 117

females): 63 staff for Bishkek RCVDE and 135 for the regional laboratories, and in the South

region, there is a total of 153 staff (including 70 females): 26 staff for the Osh RCVDE and

127 for the regional laboratories.

The average monthly net salaries are approximately €221 for a general manager, €164 for a

department or laboratory head, €104 for a specialist (technician) and € 65 for a technical

(support) or office staff.

In terms of per diem, staff receive €6,5 per day when travelling within the country and € 180

when travelling abroad. During discussions at the Bishkek RCVDE, the number of combined

travel days for all staff was estimated at 1,280 per year in the country and 34 days abroad

per year, with a grand total of € 14,440.

In Osh, the total number of days has been estimated at 10 days per year for internal travel,

which works out to be € 65. The cost of international travels in Osh is taken in charge by

international projects.

As a reference and to maintain a reasonable standard, continuing education for staff is

estimated to cost around 5% of the total salary. The estimation of the administration budget

is based on 20% of salary costs.

There is an ongoing programme to train laboratory staff overseas as well as within the

country. There are currently 16 laboratory scientists being trained overseas.

The analysis of the number of staff required to perform all the tests will be done in paragraph

VI.

IV.4 Estimated budgets for the North and the South regions

Since the North and South regions will operate practically independently, separate budgets

were estimated for both.

The budget for each region considers:

The capital investment, which comprises the renewal rate of buildings and premises,

vehicles, information technology (IT), office equipment, telecommunications, laboratory and

other equipment as described in paragraph III.1. The building renewal rate is the main

component of the capital investment.


27

Salaries and remunerations is described in paragraph III.3. The salaries constitute the

smallest component of the budget. This is due to the low salaries paid to the staff and also

the bulk of the staff is made of technical staff and support staff.

- Operating costs e.g. maintenance, calibration and metrology, reagents and

consumables, as well as continuing education and administration expenses etc. are

calculated as a portion of the above data.

The operating costs such as continuing education and administrative expenses are derived

from the salaries and constitutes also a small proportion of the operating costs.

The reagents and consumables costs constitute the main expense of the laboratories

followed by the costs of maintenance, calibration and metrology.

Estimated budgets for both regions are shown in table 4 and 5.

Table 4: Budget for the North region

Capital Investment 566 750 24,67%

Buildings and Premises 3 000 000 1 0,05 150 000 6,53%

Vehicles 10 000 15 0,2 30 000 1,31%

IT and Office Equipment 1 000 75 0,33 24 750 1,08%

Telecommunication Equipment 1 000 10 0,2 2 000 0,09%

Refrigerators & Deep Freezers (-20°C & -80°C) 0,1 - 0,00%

Laboratory Equipment 3 600 000 1 0,1 360 000 15,67%

Other Equipment 0,2 - 0,00%

Salaries and Remuneration 178 760 7,78%

Technical staff 780 40 31 200

Specialists 1 248 58 72 384 3,15%

department heads 1 966 26 51 116 2,23%

directors 2 652 9 23 868

Support Staff 780 29 22 620 0,98%

Per diem and travel allowance in the country 1 440 1 1 440 0,06%

Per diem and travel allowance abroad - 0,00%

Operating Costs 1 551 635 67,55%

Continuing Education (short courses, etc.) Salaries 5% 8 928 0,39%

Administrative Expenditures (office supplies, etc.) Salaries 30% 60 356 2,63%

Reagents and Consumables 762 000 1 762 000 33,17%

Maintenance, Calibration and Metrology Laboratory

Equipment

20%

720 000 31,34%

External Services (Reference Laboratory, External

Analysis, Transport, etc.)

350 100%

350 0,02%

Other - 0,00%

Total 2 297 145 100,00%

Renewal

Rate Annual Budget %

Kyrgyz Republic North Region (Bishkek)9

laboratories Unit Cost Number

If the virology section becomes BSL3 the budget will increase by E45000 as mentioned in

paragraph III.1.1


28

Table 5: Budget for the South region

Capital Investment 246,048

Buildings and Premises 782,950 1 0.05 39,148

Vehicles 10,000 13 0.2 26,000

IT and Office Equipment 1,000 32 0.33 10,560

Telecommunication Equipment 1,000 11 0.2 2,200

Refrigerators & Deep Freezers (-20°C & -80°C) 0.1 -

Laboratory Equipment 1,681,400 1 0.1 168,140

Other Equipment 0.2 -

Salaries and Remuneration 135,417

Technical staff 780 16 12,480

Specialists 1,248 62 77,376

department heads 1,966 16 31,456

directors 2,652 10 26,520

Support Staff 780 18 14,040

Per diem and travel allowance in the country 65 1 65

Per diem and travel allowance abroad -

Operating Costs 1,325,448

Continuing Education (short courses, etc.) Salaries 5% 7,391.60

Administrative Expenditures (office supplies, etc.) Salaries 30% 48,562

Reagents and Consumables 932,215 1 932,215


Equipment

20%

336,280



1,000 100%

1,000

Other -

Total 1,706,913

Renewal

Rate Annual Budget

Kyrgyz Republic South Region 10


It is essential to emphasize on the fact that the figures are estimation to give an indication on

how a budget is calculated. The OIE PVS team has used estimation for basically all data.

V. Sustainability of the budget: estimated cost of tests and proposed tariffs

V.1 Estimation of costs of tests

In any laboratory, the real cost of laboratory analysis depends on relative factors, such as the

number of tests performed by technique and the relative value of each test (which is a

theoretical calculation based on manpower, reagents and the use of equipment for each

test).

In the Calculation Tool, in Annex 6, the “cost of analysis” sheet gives an estimate of the cost

of each test (in Euros (€)), taking into account the number of tests, their relative value (f

column in the Tariff estimation sheet), the standard reagent cost in the country (column o in

the Cost of Analysis sheet) and the proposed annual laboratory budget (excluding reagents

and external services budget lines). The total number of points (value w in the cost of

analysis sheet) is the total number of each tests multiplied by their relative value f).

The relative value of one point is the annual budget excluding reagents and external services

divide by the total number of tests (“estimated cost of analysis” sheet in the calculation tool).


29

The estimated test cost is the sum of the reagent cost, the cost of sampling and local

transport and the relative value f multiplied by the value of one point u.

The lower the value of one point, the higher is the cost of analysis.

The calculation clearly demonstrates that the cost per laboratory analysis, when only a small

number of tests are conducted in each laboratory, is very high.

V.2 Estimation of tariffs of tests

In order to appropriately manage a laboratory, one should be able to determine a tariff based

on the national costs of manpower, equipment and reagents. This enables the laboratory to

propose an appropriate tariff to clients based on its comparative advantages (for instance,

lower cost of manpower) or disadvantages (for instance, higher cost of equipment due to

importation). Finally, this enables the country to analyse the sustainability of the laboratory

network and the possibility to apply partial or total cost recovery methods as compared to

international and national markets.

The calculation tool in Annex 6 estimates a possible national tariff (in Euros (€)) for veterinary

laboratory analyses based on proposed average international tariffs, and the share of costs

between reagents, equipment and staff and is shown on table 6.

Table 6: Tariff estimation.

Type of Analysis

Proposed

Standard

Reagent

Cost

in €

(h)

Proposed

Standard

Share of

Cost:

Reagents

(i)

Proposed

Standard

Share of

Cost:

Equipment

(j)

Proposed

Standard

Share of

Cost:

Staff

(k)

Costs of

Sampling

Kits &

Local

Transport

(l)

Examples

of

Reagent

Costs in

the

country

in €

(n)

Estimated

Reagent

Cost in the

country

in €

(o)

Proposed

Tariff

in €

(p)

Agent Identification

Parasitology 3 0.20 0.10 0.70 0.10 0.00 0.001 1.82

Bacteriology 7.5 0.30 0.20 0.50 0.10 0.00 0.001 5.36

PCR or RTPCR 16 0.40 0.30 0.30 0.10 8.35 20.70

Serology 0.10

ELISA 3 0.50 0.20 0.30 0.10 3.00 3 4.34

CF 1.8 0.30 0.10 0.60 0.10 1.00 0.05 0.82

AGID 1.2 0.30 0.10 0.60 0.10 0.01 0.01 0.56

IFA 3 0.30 0.20 0.50 0.10 0.10 0.1 2.30

BBAT 0.3 0.30 0.10 0.60 0.10 0.02 0.017 0.23

AGGL/ASCOLI 0.6 0.30 0.10 0.60 0.10 0.03 0.03 0.35

MAT 12 0.60 0.20 0.20 0.10 0.01 0.01 4.19

Other Tests 0.10

HPLC/LC-MS 17 0.20 0.60 0.30 0.10 10.00 10 61.63

CPG (GC) 14 0.20 0.60 0.30 0.10 10.00 10 52.54

Spectrophotometry 2.5 0.10 0.50 0.40 0.10 10.00 10 22.81

Food Microbiology Standard 5

Parameters 24 0.40 0.10 0.50 0.10 12.52 9.67

The tariff estimation for FS will have to be reviewed once the country obtains an exact value

for the cost of reagents and decides on a more accurate number of tests. As mentioned

previously the government can choose to partially subsidise the tests or not.


30

There are a number of tests mentioned in the central laboratories which have not been taken

into account: organoleptic analysis, biochemistry analysis: the Qualitative antibiotic detection

should be replaced by modern technology (LC-MS). Biochemistry and organoleptic tests are

not considered by OIE to be part of the services a national laboratory should offer. Such tests

can be performed in local markets by veterinary inspectors with some laboratory skills or by

private laboratories.


31

VI. Reorganisation of the Kyrgyz veterinary laboratory network and estimation of number of staff required

VI.1 Central laboratories - RCVDES

The two central laboratories comprise each the following 9 technical departments:

Bacteriology

Virology

Serology

Radiology

Chemical-toxicology

Ascoli

Parasitology

Leptospirosis

Leucosis

The number of departments should be reduced as there is no reason to separate Leucosis

and Ascoli and Leptospirosis from serology. Therefore, Leucosis, Leptospirosis and Ascoli

should be under the Serology department. The bacteriology department will perform all

bacteriological testing of food stuff previously under Food safety. Furthermore, radiology and

chemical-toxicology departments can be combined under the Food Safety department.

In a laboratory processing a high number of samples, there should theoretically be an ELISA

reader and equipment for molecular biology in virology, bacteriology, parasitology and FS.

However, considering the number of samples to be processed, it would be far more efficient

to centralise all ELISA testing in the serology section.

In order to optimize the management system, while avoiding a large number of laboratory

heads and maximizing cost effectiveness, it is suggested to have 5 departments:

Bacteriology performing conventional bacterial identification and isolation,

leptospirosis testing, food bacteriology and also managing all autopsies

Parasitology performing microscopic examination of fecal samples and blood

smears for parasites

Serology performing all ELISA tests (bacterial, virological and

parasitological), brucellosis testing, AGID (e.g. Leucosis) and Ascoli, MAT

(Leptospirosis)

Virology and Molecular Diagnostics performing IFAT (for rabies) and all PCR

and qPCR.

Chemistry for all residues in food stuff.

In order to avoid contamination when performing PCR or qPCR it is essential to separate

“clean” and “dirty” areas. Therefore, there should be 4 or 5 rooms allocated to the different

stages of molecular diagnostic by PCR (sample processing and nucleic acid extraction in a

biosafety cabinet, reagent and master mix preparation in a biosafety cabinet, template

addition to the mastermix in a biosafety cabinet, running of PCR and post-PCR tests). The

OIE PVS Laboratoryy Pathway Team noted that both central laboratories have already set

up adequate PCR facilities and required equipment, including a robot to extract DNA in the


32

FS laboratory. However, if these facilities were used solely for the purpose of meat origin

identification and GMO detection, they would be vastly underutilised. Given that molecular

techniques can be used for bacterial, viral and parasitological identification, there is no need

in the near future to set up second facilities for that purpose, hence it is suggested that all

PCR testing remain in the existing rooms in the FS department but under the supervision of

the virology and molecular diagnostics head of department.

Although theoretically viruses can be identified by PCR and qPCR techniques, virus isolation

techniques by cell culture may be required if the PCR doesn’t show specific amplification of

target genes. Therefore virus isolation may be required, however this demands specific

technical expertise and considerable financial investment. . Since there is a memorandum of

understanding in place between the State Inspectorate on Veterinary and Phytosanitary

Security, under the Government of the Kyrgyz Republic and the Veterinary Research

Institute, it is strongly suggested to have these tests be performed by the VRI. This would be

more cost-effective than creating costly BSL3 laboratories both from a financial as well as

from an efficiency point of view.

VI.2 Regional laboratories

Tests requiring a low level of technology such as CFT, AGID, and microscopy can be

performed in regional laboratories. However technicians performing these tests should be

properly trained and supervised and there should be a good documented quality control for

all reagents. During discussions with various SIVPS staff there was a mention of some

regional laboratories performing ELISA techniques. This technique requires a high level of

skills and should only be performed by qualified and trained staff using regularly maintained

and calibrated equipment and according to controlled methods. Moreover, it is impossible to

maintain skills and expertise in a variety of techniques if samples are not submitted routinely.

The techniques and equipment used will not be put into practice/use on a basis regular

enough to be sufficient for maintaining proficiency of staff and accuracy of results.

Considering also that the ELISA technology as well as molecular techniques are high

throughput techniques which allow thousands of samples to be processed per day, it is more

efficient to centralise all ELISA testing and molecular techniques in both RCDVEs.

Therefore, it would be more cost-effective if regional laboratories perfom only necropsies,

parasitology examination, BBAT, CFT and microcopic examination of slides for bacteriology

and parasitology. Some regional laboratories might perform a few simple preliminary

bacteriological tests e.g. bacterial isolation before sending either original samples and/or

bacterial isolates to their correponding central laboratories for confirmation and identification

of pathogens.

VI.3 Estimation of number of staff required by the veterinary laboratory network

VI.3.1 Central laboratories

In order to assess the number of staff required to process all tests the OIE PVS Pathway

Laboratory Team referred to a laboratory from an neighbour ex USSR country, performing a

total of 2 Million tests, and approximately the same number of tests for Food Safety as


33

suggested in the coming years in the Kyrgyz Republic, with the same equipment. In this

country, there is a total of 85 staff in the central laboratory including directors, head of

departments, technical and support staff. Among these, 12 staff members perform FS

testing.

The total number of staff in the Bishkek RCVDE is currently 63 and in the Osh RCVDE 26. It

is anticipated that the Osh RCDVE will perform more tests than the Bishkek RCVDE.

Therefore, it can be suggested that the total number of staff at the national level in the two

RCDVE is sufficient for the performance of all tests, but it will be necessary to increase the

number of staff in Osh, especially for the FS programme. Based on the present predictions, 6

staff in each central laboratory should be sufficient to perform all FS testing until the time

when the number of tests increases significantly.

In the context of the Kyrgyz Republic, where a lot of livestock move to high pasture from May

to September and the number of samples for serology purpose is considerably reduced

during 5 months of the year, it seems essential that staff is trained to perform several

techniques so they can be re-allocated temporarily to different divisions if and when required.

It is worth considering allocating these staff to FS testing, especially for residue testing of

samples. These staff will not be skilled enough for the handling of LC-MS or GC equipment,

which require considerable expertise, but they can be trained to performed initial processing

of samples for food safety testing.

VI.3.2 Regional laboratories

Discussion with staff in a regional laboratory indicated that one technician is enough to

perform 1,500 BBAT per day, or 250-300 CFT per day or examine 25 microscopic slides per

day for parasitology.The present demand for tests as shown in Annex 1 and the prospective

demand for Official programmes as shown in table 6 show that 4 staff with veterinary training

per regional laboratory are sufficient to perform necropsies, laboratory tasks and

administrative tasks. Taking into account sick leave, maternity leave etc. the number could

be increased to a total of 6 staff per regional laboratory, which will work out to be 102

technicians for the 17 regional laboratories. There should also be 1 director and 1 support

staff in each laboratory making a total of 136 staff. Presently there is a total of 100 staff

(including the directors) in the regional laboratories in the North region and 93 staff (including

the directors ) in the regional laboratories in the South region. There is a need to rationalise

the staff allocation across the Veterinary Laboratory Network, train staff o multitask and avoid

overstaffing.

Detailed staff management is discussed in paragraph VII.2

VI.4 Training and on-going education

Staff in the regional laboratories perform old techniques with poor specificity (such as Serum

agglutination for Brucellosis) and are not regularly trained and audited. It is very important to

establish training programmes at all levels to ensure the reliability and the quality of testing.

The Veterinary Scientific board established by Veterinary Inspectorate could be a useful

resource. Furthermore, the Veterinary board could also be considered in any event where


34

some technical problems are encountered e.g. it is unavoidable that scientists in the central

laboratories encounter problems when using molecular techniques.

The Veterinary Research Institute could be a useful resource. Furthermore, the Veterinary

Research Institute could also be considered in any event where some technical problems are

encountered e.g. it is unavoidable that scientists in the central laboratories encounter

problems when using molecular techniques. Once again the Research Institute of Veterinary

should be a useful resource. Similarly, the VLN should consider other training opportunities,

including training overseas, in all disciplines for all staff across the network.


35

VII Optimisation of the VLN in the next 5 years

VII.1 Equipment and sample collection

Based on discussions the OIE PVS Pathway Laboratory Team had with various authorities, it

was decided that a stocktake of the equipment located in the laboratories that are due to be

closed should be undertaken. At the same time, an inventory of all the equipment located in

all the laboratories should be also undertaken to assess the equipment needs of all the

laboratories. Once the inventory has been finalised, the equipment should be categorised

into obsolete equipment (need to be disposed of), equipment in working order requiring

either repairs or servicing and equipment in good condition. Further to this assessment, the

relocation of equipment in working order and equipment in good condition should be

considered according to the needs of each laboratory. During this process, each equipment

needs to be individually identified with a tag and a unique number and a list of equipment

must be drawn Furthermore each equipment should have a clear calibration / maintenance

date to meet quality assurance requirements.

The use of glassware for all the tests performed including sampling of blood, petri dishes,

glass pipettes, etc. is highly labour intensive and requires extra staff members who spend

lots of time cleaning and sterilising the glassware. This point has been already mentioned in

other PVS Pathway reports where it was recommended that the laboratories consider

switching to the use of single-use consumables. The OIE PVS Laboratory Pathway Team

supports this recommendation as that would make the laboratory operations more cost

effective.

VII.2 Establish efficient human resources management

VII.2.1 Establish private laboratories

There is an excess of staff in both regions as shown in paragraph VI. The government could

offer incentive for early retirement or financial help to establish private laboratories which

could progressively take over the clinical tests under the supervision of the central

laboratories. Furthermore, staff choosing to establish private laboratories, would be provided

with tailored training on selected techniques in the central laboratories. This training could be

also tailored as a continuing education programme. The private laboratories can also fulfil the

role of sample collection and transportation “agents” for the Veterinary Laboratory Network.

Since a number of laboratories are supposed to close down, the SIVPS should consider the

lease of the buildings of the closed laboratories to staff considering starting up private

laboratories.

VII.2.2 Develop awareness programmes

Regional laboratories staff are mainly busy in the January to May period, when the

surveillance programme for brucellosis generates several thousands of serum samples for

BBAT. During the period of low laboratory testing throughput, laboratory staff can conduct an

outreach awareness campaign for farmers, producers and other potential stakeholders on

disease recognition, efficient use of the laboratory and interpretation of laboratory tests. This

could favour the establishment of good relationships and trust between producers and


36

veterinary laboratories and generate higher numbers of samples submitted to the VLN, which

in turn will maintain staff competency.

VII.3 Technical expertise and Quality Assurance

Although OIE usually recommends a strict chain of command from one central laboratory to

regional laboratories, the OIE PVS Pathway Laboratory team acknowledges that in the

context of the Kyrgyz Republic, the existence of two independent central laboratories is

justified. This has been endorsed at the highest government level. Each central laboratory

supervises a number of regional laboratories. However it is imperative to create an

independent technical authority which would supervise both regions. The board of specialists

should include staff from the Veterinary Research Institute and staff with the required

technical expertise in various disciplines e.g. virology, bacteriology, parasitology and

molecular techniques.

Any veterinary laboratory that provides a service is required to have a quality assurance

system in place. The OIE PVS Pathway Laboratory Team noted that the 2 central

laboratories have in place a quality assurance system with written and controlled standard

operating procedures and the use of quality control in various assays. However there is no

quality system in place for media preparation in all sections: for example in bacteriology

where all media are prepared locally, there is no media quality control with reference strains.

Furthermore, the requirements of quality assurance include conducting internal and external

audits on a regular basis, however, the OIE PVS Pathway Laboratory Team noted that there

were no internal and external audit systems.

Regional laboratory staff should be given opportunities to upgrade their skills by

spending some time in the central laboratories, where they will go a training based on

pre-developed training programmes based on national needs as determined by the

central laboratories in conjunction with the Veterinary Research Institute.

The collaboration between the Veterinary Research Institute and the Bishkek RCVDE,

which is based on a Memorandum of Understanding between the 2 institutions, should

be extended to the Osh RCDVE.

Since the control of Echinococcosis is a priority at the national level, both central

laboratories should work with the research institute to adopt and validate a published

PCR technique for the identification of the parasites.


37

VII.4 Establish relevant data management

All laboratories have registries for samples received and to record results. At present all data

are sent to the Bishkek RCVDE which do not have a clear and consistent data management

system; therefore their data, mostly paper based, are difficult to analyse and follow in real

time. The VLN should consider transferring their data management system to a computer-

based system, or LIMS, to facilitate data analysis, inform decision making and assist in the

development of rationalised strategies within laboratory resource allocations.

VII.5 Establish a relevant tariff for all tests

It is essential that a laboratory network has one independent budget, even if the government or international organisations are the main source of funding and also the main clients for the official programmes. Both RCDVEs should have a clearly defined budget.

For laboratory activities to be sustainable, the following needs to happen:

All clients must be identified: international projects, government, commercial farms,

smallholders, food processing companies, etc.;

Tariffs must be established for all clients;

Tariff must be documented and adjusted according the client potential income;

Tariffs should be lower for small holders and will need to be sponsored by government subsidies.

The present analysis doesn't take into account biochemistry, organoleptic tests, qualitative antibiotic residue testing which are not the scope of a national laboratory. Such tests should be performed by private laboratories.

VII.6 Further reduction the number of regional laboratories

Once a network of private laboratories is implemented, the government should be able to

consider reducing the number of regional laboratories. Based on geographical constraints,

this number should be between 8 and 10 for the whole country. The cost of buildings and

infrastructure maintenance could be reduced by half, as well as staff number.

If not privatised, some laboratories could be transformed into reception points, primary

processing, storage and delivery of samples of biological material to central or regional

laboratories for testing. Such reception points will require only one staff part-time with

minimal training.

Table 7 and 8 show the budgets for a total of 10 laboratories performing solely tests for the

official programmes, Ascoli and FS with an optimised number of staff. The budget would be

reduced by approximately €200 000 in the South and €300 000 in the North

If the demand increases in the coming years, the cost of reagents will increase but it should

be covered by applying appropriate tariffs.

Although during the Gap Analysis PVS mission, there was a suggestion of reducing the total

number of regional laboratories down to 5, discussions with the CVO during this mission

indicated that this would not happen in the next 5 or 6 years, therefore this option was not

analysed and budgeted for.


38

Table 7: Budget for a network of 1 central laboratory and 5 regional laboratories in the

South region


Buildings and Premises 591,000 1 0.05 29,550

Vehicles 10,000 7 0.2 14,000










directors 2,652 6 15,912









Equipment

20%

307,000



1,000 100%

1,000

Other -

Total 1,537,705

Renewal

Rate Annual BudgetKyrgyz Republic Osh Region 5 laboratories Unit Cost Number


39

Table 8: Budget for a network of 1 central laboratory and 5 regional laboratories in the

North region


Buildings and Premises 2,300,000 1 0.05 115,000

Vehicles 10,000 7 0.2 14,000










directors 2,652 6 15,912









Equipment

20%

700,000



1,000 100%

1,000

Other -

Total 2,012,655

Renewal

Rate Annual Budget

Kyrgyz Republic Bishkek Region 5 regional



40


41

Conclusions

The objective of the OIE PVS Pathway Laboratory Mission in the Kyrgyz Republic was to

provide national decision makers with information on how to allocate appropriate resources

to the VLN and to make strategic decisions to support accurate and timely diagnosis.

The OIE PVS Pathway team analysed the sustainability of the national Veterinary Laboratory

Network in the Kyrgyz Republic where there is no private sector to perform laboratory

analysis. Presently, all tests for official veterinary programmes, clinical tests and FS are done

in the state own laboratories.

The opportunities to increase the number of tests and to conduct community outreach in

order to develop the demand for laboratory analysis by livestock small holders were

discussed.

The optimisation of the number of staff, their training and possible relocation were examined

as well as the creation of a network of private laboratories to perform all clinical and

biochemical tests.

In the view of the OIE PVS Pathway Laboratory Team a further reduction in the next coming

years of the number of laboratories would present substantial savings. The redundant

laboratory buildings could be leased to private veterinaries with laboratory expertise (e.g.

former laboratory staff) to develop private laboratories.

It will be essential to implement a thorough quality control system in all laboratories and a

regular ongoing training programme at all levels.

There is a support and understanding from the Veterinary Authority for the improvements

and changes to be brought to the national laboratory network considering the interest they

devoted to the OIE PVS Pathway and the activities initiated by government in view of

improving Veterinary Services.

This PVS Pathway mission should pave the way for a more rapid implementation of different

activities, mentioned above, which are proposed taking into account the activities already

initiated by the Kyrgyz Republic in view of improving their laboratory network.


42


43

Annexes

Annex 1: Data Recapitulation table for all laboratories of

the national network

Annex 2: Main events and timeline of the mission

Annex 3: Analytical Line Tool (equipment required in the

regional labs)

Annex 4: Calculation of reagent cost

Annex 5: Demand Tool

Annex 6: Calculation Tool


44


45

Annex 1: Data Recapitulation table for all laboratories of the national network

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12 Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19

Number of Technical staff 27 4 1 1 2 2 1 1 1 1 2 2 2 2 2 2 1 1 1

Number of Specialists 7 13 4 5 8 6 6 5 5 3 7 9 9 6 7 8 4 4 4

Number of department

heads 10 4 1 1 2 2 1 1 1 1 2 3 3 2 3 2 1 1 1

Number of directors 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

Number of technical and

office staff 17 4 1 1 1 3 1 1 2 2 2 2 2 2 1 2 1 1 1

Total number 63 26 8 9 14 14 10 9 10 8 14 17 17 13 14 15 8 8 8

Female 37 13 2 4 9 7 5 4 6 6 10 10 9 10 9 6 5 6

Number IT\phone 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

Number of Cars 4 2 1 1 2 1 2 1 1 2 2 2 2 1 1 1 1 1

Number of Computers 51 9 2 3 2 3 1 2 3 3 4 4 3 4 3 3 3 1 3

0

Surface of Buildings in m2 Total Surface in sq m2Average Value in Euros per m2Total value in Euros

surface BSL1 847 1244 750 70 4780 258 1158 234 453 198 330 357 1607 226 1306 700 314 314 15146 500 € 7 573 000 €

surface BSL2 250 250 1 000 € 250 000 €

surface BSL3 250 250 5 000 € 1 250 000 €

Total cost for buildings 9 073 000 €

Value of Equipment in Euros 1 370 000 € 1 352 000 € 25000 23000 28 000 € 58 000 € 20 000 € 25 000 € 37 000 € 41000 37 000 € 46 000 € 40 000 € 34 000 € 13 000 € 45 000 € 54 000 € 44 000 € 54 000 € 3 346 000 €


46


47

Annex 2: Main events and timeline of the mission Date Location/meetings Activities

Monday 26th

June

Bishkek: meetings Meeting with Dr Kalysbek Zhumakanov, CVO, State

Inspectorate on Veterinary and Phytosanitary Safety under the

Government of the Kyrgyz Republic

Meeting with Dr Joldoshbek Kasymbekov, OIE Focal Point for

laboratories, Agricultural Project Implementation Unit

Ms Chynara Aalieva, CVO assistant, State Inspectorate

Dr Alimbekov Shabdan, Chief specialist, Republican centre for

Veterinary Diagnosis and expertise

Dr Ermek Kurmankulov, Head of Department, Republican

Centre for Veterinary Diagnosis and expertise

Dr Shamen Sydykov, Deputy Head, Bishkek city Inspectorate

Dr Zamir, Deputy Head, Alamudin rayon Inspectorate

Tuesday

27th June

Bishkek, State

Inspectorate on

Veterinary and

Phytosanitary

Safety under the

Government of the

Kyrgyz Republic

Bishkek,

Republican centre

for Veterinary

Diagnosis and

Expertise with all

heads of the

departments

Meeting with Dr Kalysbek Zhumakanov, CVO, State



Ms Chynara Aalieva, CVO assistant State Inspectorate

Dr Toroev Mamarasul, Head of Republican centre for

Veterinary Diagnosis and Expertise

Dr Kydyrgychev Asan, Deputy Director,

Dr Toimbetov Raxim, Head of the leucosis department

Dr Joldoshbek Kasymbekov, OIE Focal Point for laboratories,

Agricultural project Implementation Unit

Dr Alimbekov Shabdan, Chief specialist

Meeting in the Republican centre for Veterinary Diagnosis and

expertise with all heads of the departments:

Dr Kysaibekov Suiunbubu, head of serological department

Dr Kurmankulov Ermek, head of chemical toxicological

department

Dr Shadyrbekova Dinara, head of radiological department

Dr Musayeva Munara, chief specialist of the virology

department


48

Dr Saskarayeva Chynara, head of the department leptospirosis

diagnostic

Dr Sultalomov Zhanbolot, head of the Askoli department

Dr Urusov Kazbych, head of the parasitological department

Wednesday

28th June

Meetings in

Bishkek

Republican centre

for Veterinary

Diagnosis and

Expertise

Dr Kydyrgychev Asan, Deputy Head of Republican centre for

Veterinary Diagnosis and expertise

Dr Toimbetov Rahim, Head of the leucosis department



Agricultural Project Implementation Unit

Meeting in the Republican centre for Veterinary Diagnosis and

expertise with all heads of the departments


Dr Kurmankulov Ermek, Head of chemical toxicological

department

Dr Shadyrbekova Dynara, head of radiological department

Dr Musayeva Munara, chief specialist of the virology

department

Dr Saskarayeva Chynara, head of the department leptospirosis

diagnostic

Dr Sultanaliev Zhanbolot, head of the Askoli department


Thursday

29th June

Meetings in

Bishkek

Dr Kadyrgychev Asan, Deputy Head of Republican Centre for




Dr Toimbetov Rahim, Head of the leucosis department


Dr Kurmankulov Ermek, Head of chemical toxicological

department




49

Friday 30th

June

Meetings in

Bishkek

State Inspectorate

on Veterinary and

Phytosanitary

Safety under the

Government of the

Kyrgyz Republic

Dr Kalysbek Zhumakanov, CVO, State Inspectorate on

Veterinary and Phytosanitary Safety under the Government of

the Kyrgyz Republic



Dr Sotovaldiyev Adylet, inspector

Dr Abdrayev Murat, head of department

Dr Tabaldiyev Tilek, Inspector of the unit of internal supervision

Dr Iskembayeva Gulmairam, Inspector of the unit of internal

supervision

Dr Kuttubek kyzy Gulzada, Inspector of the internal

supervision unit

Saturday 1st

July

Travel to Osh

Meeting in the

Regional Center for

Veterinary

Diagnostic and

Expertise in Osh



Dr Abdymomunov Islam, director of Regional Center for

Veterinary Diagnostic and Expertise in Osh

Dr Sulaimanov Astan, head of VS administration of Kara-Suu

district

Dr Pahradinova Akshagul, head of the veterinary and sanitary

expertise and chemical toxicological test

Dr Dolotbayev Nyrgazy, chief specialist

Dr Syrgabayev Danyar, leading specialist,

Dr Bakayeva Aigul, leading specialist

Dr Gaipkulov Abdyrazak, leading specialist

Dr Nabigysan uglu Nyrbek, leading specialist

Dr Primkulova Burul, specialist

Dr Gorelov Viacheslav, head of radiological department

Dr Mamatov Abdybaky, head of bacteriological department

Dr Lubimova Valentyna, chief specialist

Dr Matkasymova Maksuda, technical staff,


50

Dr Gaprdjalova Oglay, technical staff

Dr Ashyrbayeva Arzykan, technical staff of parasitology

department

Dr Hasanova Tota, specialist

Dr Caydilda uglu Kalysbek, specialist of serological

department

Dr Ashyaram kyzy Kelsinay

Dr Sotarova A, specialst

Dr Alymdjanova B. Head of virology department

Dr Atokozyeva G. Chief Specialist

Sunday 2nd

July

Meetings in Osh





Dr Kaarov Emil, head of the VS in the Nookat region

Dr Sulaimanov Astan, head of VS administration of Kara-Suu

district

Dr Kochkorov Zhenysh, veterinarian in the Nookat region

Dr Sabyrov Imaraly, veterinarian in the Nookat region

Dr Ibragymov Zamyrbek, veterinary specialist in the Nookat

region

Monday 3rd

July

Meetings in Osh





Dr Sulaimanov Arstan, head of VS administration of Karasyisk

district

Travel to Bishkek


51

Tuesday 4th July

Meetings in Bishkek Meeting in the Kyrgyz research institute of veterinary after A. Duysheev

Dr Akmatova E., director

Dr Dardykina E., scientific secretary,

Dr Kryzskaya E., virology department

Dr Nurgazyeva A., head of the virology department

Dr Tursunov T., head of the parasitology department

Dr Tolybayeva M., virology department

Dr Chegyrov S., brucellosis department

Dr Isayev M., specialist

OIE PVS Pathway Laboratory brain storming session

Wednesday

5th July

Meetings in

Bishkek

Finalisation of various options

Presentation of preliminary findings and options in a general

meeting

Meeting with Dr Kalysbek Zhumakanov, CVO, State





Dr Toroev Mamarasul, Head of Republican centre for


Thursday

6th July

Meetings in

Bishkek

Report writing

Friday 7th

July

Meetings in

Bishkek and

Tokmok

Meeting in the Chuiskyi Interdistrict Center for Veterinary

Diagnostic and Expertise (in Tokmok)

Dr Baktybek Asanaliyev, director of the center

Dr Dinara Sultanova, head of the bacteriological department

Dr Zhyldyz Zhaparova, specialist parasitology department

Dr Usen Zhusubakunov, head of the serology department

Report writing

Saturday 8th

July

Departure


52


53

Annex 3: Analytical Line Tool (equipment required in the regional laboratories)

Equipment Unit Price in

Euros

Number

of units

Total in

Euros

Microscope 2,000 2 4,000

Incubator 2,000 2 4,000

Autoclave 6,000 1 6,000

Water bath 1,000 2 2,000

Balance 1,000 2 2,000

Distillator 2,000 1 1,000

Fridge 1,000 2 2,000

Freezer 2,000 2 2,000

Centrifuge 4,000 2 8,000

Drying oven 1,500 1 1,500

Shaker 1,500 1 1,500

pH Meter 1,000 1 1,000

Magnetic

stirrer 1,000 2 1,000

Pipettes 200 3 600

Total 36,600

Total for 17

laboratories 622,200


54


55

Annex 4: Calculation of reagent cost

Test Kits/

Reagents

Cost /kit (ml) Note Cost of 1

test

BBAT BBAT

reagent

1300 S/100ml 3300 reactions at 30ul

per reaction

0,30 per dose so 0.005

€ per dose

€0,017

AGID

Kit with Agar

“Difko

Used for leucosis

testing

€0,01

CFT Reagents:

Brucella

Antigen,

positive and

negative

serum,

hemolisinum,

complement

Antigen

392 S/100 ml,

Hemolisinum 4000

doses /3120 S

Positive serum/65

S/1,0 ml

Negative serum\80

S/1,0 ml

Complement

/2000 dose/1400

som

Antigen is used

(working solution)1:75

Complement is used (in

dilution) 1:20

€0,05

Ring Test 20ml for 200 tests=

440

€0,03

AGID (sheep

pox)

Kit with Agar

“Difko”

1 kit =22 000som 1 kit \250 tests €1,13

ELISA TESTS For each of the ELISA kits for which invoices were available the calculation

of the cost was estimated by adding €0.1, which is the cost of 10

micropipette tips per sample.

ELISA PPR 1 kit = 24 000som

1 kit\32 samples

1 kit\88 tests € 3,5

ELISA leucosis 1000\14950 som €0,2

ELISA

Chloramphenic

ol

27550

som\ plate

€3,7

ELISA Aujesky €1,2

ELISA Horse

infection

arthritis

€3,4

ELISA for Foot

and Mouth

Disease (NSP

ELISA)

122000s/5

plates

92 samples/plate

Total

460samples=265so

m/sample

€3,4


56

ELISA for Foot

and Mouth

Disease (SP

ELISA)

vaccination

S19700

for 168 samples

117s/sample

Duplicate samples €1.5

Cost of tips 0.89 S

ASCOLI TEST Precipitate

serum

1litre\21000 som =

250 € and allows

10,000 tests,

the cost of each test

is 0,025€

The cost of the acid

phenol solution is

almost negligible and

has been estimated to

0,01€

€0,035

MAT

(leptospirosis)

€0,01

IFA (Rabies)

Immuno

fluorescen

t serum

1 ampul\2100 som Working solution 1:8

0,2 ml per sample

0,03 ml\one test

€0,1


57

Annex 5: Demand Tool

North region

Para

sito

logy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID L

EU

CO

SIS

AG

ID P

OX

IFA

BB

AT

Pre

cip

itatio

n (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1

2.1.1. 125000 21 875

2.1.4. 7000 1 680

Foot and mouth disease (NSP 4200

2.1.5. 2800 4 592

2.1.9. 14000 2 100

2.1.13. 175 42

2.2.2. 2065 2 354

2.2.3. 2065 2 3542.2.4. 2065 496

2.3.4. 700 #REF!

2.4.3. 42000 420000 15000 76 470

2.4.11. 32500 4 875

2.5.3. 9870 1 875

2.5.11. 9870 #REF!

2.7.11. 21000 76 440

2.7.14. 10500 #REF!

General parasitology 140000

General Bacteriology 12000

other ELISA 1600

6000 6 840

10000 1 10015300 16 830

2000 15300 126 210

15300 123 930

15300 169 830

7000 35 980

35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238

149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000Total Number of Tests (n)

Standard 5 bacteria (TPC, Ecoli, Salm,List, Staph)

Antibiotics

Hormones and anabolics

Pesticides

Cost by Test:

Consumables for National Laboratory n * (a + b + d))

GMO

Radiological tests

HeavyMetals

Glanders

Peste des petits ruminants

Sheep pox and goat pox

Avian influenza

Dourine

Enzootic bovine leukosis

brucellosis all species

Laboratory Tests

(a) Parametric cost (in €) of sampling kits (needle, tubes, etc.)

(b) Parametric cost (in €) of consumables (reagents, kits, etc.)

Leptospirosis

Unit cost of sampling kits and local delivery (a + d)

960 610

Unit cost of laboratory test in country: reagents, sampling

kits and local delivery (a + b + d)

OIE Listed Animal Diseases (as of May 2013)

Food Safety Analysis

Anthrax

Echinococcosis / hydatidosis

Foot and mouth disease ( SP)

Nosemosis of honey bees

American foulbrood of honey bees

European foulbrood of honey bees

Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antib

iotiq

ues,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

estic

ides,p

estic

ides)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)

OIE Animal Health Diagnostic Tests

(as stated in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals)

Number of Tests

Serology

OIE

Manual C

hapte

r N

um

ber

Agent

Identification

(d) Parametric cost (in €) of local transport for 2 Kg

(25 samples)


58

Para

sito

logy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID L

EU

CO

SIS

AG

ID P

OX

IFA

BB

AT

Pre

cip

itatio

n (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1

2.1.1. 125000 21 875

2.1.2. 0

2.1.4. 7000 1 680


2.1.5. 2800 4 592

2.1.9. 14000 2 100

2.1.13. 175 42

2.2.2. 2065 2 354

2.2.3. 2065 2 354

2.2.4. 2065 496

2.2.5. 0

2.2.6. 0

2.2.7 0

2.3.2. 0

2.3.4. 700 #REF!

2.3.9. 0

2.3.11. #REF!

2.3.12. #REF!

2.3.14. #REF!

2.4.3. 42000 420000 15000 76 470

2.4.11. 32500 4 875

2.5.3. 9870 1 875

2.5.10. #REF!

2.5.11. 9870 #REF!

2.7.11. 21000 76 440

2.7.14. 10500 #REF!

2.8.8. #REF!



other ELISA 1600

6000 6 840

10000 1 100

15300 16 830

0

2000 15300 126 210

15300 123 930

15300 169 830

0

7000 35 980

35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238

149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000

Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antib

iotiq

ues,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

estic

ides,p

estic

ides)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)



Number of Tests

Serology

OIE

Manual C

hapte

r N

um

ber

Agent

Identification


(25 samples)

960 610





Anthrax

Aujeszky's disease



Avian infectious bronchitis


Small hive beetle infestation (Aethina tumida)

Tropilaelaps infestation of honey bees (Tropilaelaps

Varroosis of honey bees



Infectious bursal disease (Gumboro disease)

Laboratory Tests



Leptospirosis


Newcastle disease

Fowl cholera (pasteurella all species))

Egg Drop Syndrome

Avian influenza

Dourine



Glanders


Equine viral arteritis


Swine influenza


Antibiotics


Pesticides

Cost by Test:


Mycology

GMO

Radiological tests

HeavyMetals

Total Number of Tests (n)

P

ara

sito

logy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID L

EU

CO

SIS

AG

ID P

OX

IFA

BB

AT

Pre

cip

itatio

n (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,3 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,1 1,4 0,2 0,1 0,1 3,6 0,1 1,1 0,1 8,1 11,1 1,1 0,1

2.1.1. 125000 21 875

2.1.2. 0

2.1.4. 7000 1 680


2.1.5. 2800 4 592

2.1.9. 14000 2 100

2.1.13. 175 42

2.2.2. 2065 2 354

2.2.3. 2065 2 354

2.2.4. 2065 496

2.2.5. 0

2.2.6. 0

2.2.7 0

2.3.2. 0

2.3.4. 700 #REF!

2.3.9. 0

2.3.11. #REF!

2.3.12. #REF!

2.3.14. #REF!

2.4.3. 42000 420000 15000 76 470

2.4.11. 32500 4 875

2.5.3. 9870 1 875

2.5.10. #REF!

2.5.11. 9870 #REF!

2.7.11. 21000 76 440

2.7.14. 10500 #REF!

2.8.8. #REF!



other ELISA 1600

6000 6 840

10000 1 100

15300 16 830

0

2000 15300 126 210

15300 123 930

15300 169 830

0

7000 35 980

35776 25228 35980 7222 4592 14868 11730,6 4875 15120 42 65940 21875 76440 2100 2280 2550 247860 169830 16830 1100 762 238

149065 22130 7000 2300 2800 4200 61740 32500 10500 175 420000 125000 21000 14000 2000 15000 30600 15300 15300 10000

Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antib

iotiq

ues,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

estic

ides,p

estic

ides)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)



Number of Tests

Serology

OIE

Manual C

hapte

r N

um

ber

Agent

Identification


(25 samples)

960 610





Anthrax

Aujeszky's disease











Laboratory Tests



Leptospirosis


Newcastle disease


Egg Drop Syndrome

Avian influenza

Dourine



Glanders




Swine influenza


Antibiotics


Pesticides

Cost by Test:


Mycology

GMO

Radiological tests

HeavyMetals



59

South region

Para

sito

logy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID le

ucosis

AG

ID P

OX

IFA

BB

AT

Pre

cip

itatio

n (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

15 25 40 50 6 6 6 4 10 1 2 6 20 5 1 85 70 25 30

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

350 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 14,0 87,5 87,5 87,5 87,5

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2

2.1.1. 125000 21 875

2.1.4. 13000 3 120


2.1.5. 5200 8 528

2.1.9. 26000 3 900

2.1.13. 325 78

2.2.2. 3835 4 372

2.2.3. 3835 4 372

2.2.4. 3835 920

2.3.4. 700 #REF!

2.4.3. 78000 780000 3265 137 835

2.4.11. 17500 2 625

2.5.3. 18330 3 483

2.5.11. 18330 #REF!

2.7.11. 39000 141 960

2.7.14. 19500 #REF!2.8.8. #REF!


General bacteriology 16361

other ELISA 552

6000 6 840

10000 1 116

15300 16 854

2000 15300 126 234

15300 123 954

15300 169 854

7000 35 980

41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215

171569 30031 7000 1252 5200 7800 114660 17500 19500 325 780000 125000 39000 26000 2000 3265 30600 15300 15300 10000

Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antib

iotiq

ues,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

estic

ides,p

estic

ides)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)



Number of Tests

Serology

(e) Parametric cost (in €) of international shipment 2 Kg

(25 samples)

OIE

Manual C

hapte

r N

um

ber

Agent

Identification


(25 samples)

1 421 302





Anthrax






Laboratory Tests



(c) Average price (in €) for international laboratory test

Leptospirosis


Avian influenza

Dourine



Glanders


Sheep pox and goat poxSwine influenza


Antibiotics


Pesticides

Cost by Test:


GMO

Radiological tests

HeavyMetals



60

Para

sito

logy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID le

ucosis

AG

ID P

OX

IFA

BB

AT

Pre

cip

itatio

n (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2

2.1.1. 125000 21 875

2.1.2. 0

2.1.4. 13000 3 120


2.1.5. 5200 8 528

2.1.9. 26000 3 900

2.1.13. 325 78

2.2.2. 3835 4 372

2.2.3. 3835 4 372

2.2.4. 3835 920

2.2.5. 0

2.2.6. 0

2.2.7 0

2.3.2. 0

2.3.4. 700 #REF!

2.3.9. 0

2.3.11. #REF!

2.3.12. #REF!

2.3.14. #REF!

2.4.3. 78000 780000 3265 137 835

2.4.11. 17500 2 625

2.5.3. 18330 3 483

2.5.10. #REF!

2.5.11. 18330 #REF!

2.7.11. 39000 141 960

2.7.14. 19500 #REF!

2.8.8. #REF!



other ELISA 552

6000 6 840

10000 1 116

15300 16 854

0

2000 15300 126 234

15300 123 954

15300 169 854

0

7000 35 980

41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215



Antibiotics


Pesticides

Cost by Test:


Mycology

GMO

Radiological tests

HeavyMetals

Glanders




Swine influenza

Newcastle disease


Egg Drop Syndrome

Avian influenza

Dourine



Laboratory Tests



Leptospirosis


1 421 302





Anthrax

Aujeszky's disease











Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antib

iotiq

ues,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

estic

ides,p

estic

ides)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)



Number of Tests

Serology

OIE

Manual C

hapte

r N

um

ber

Agent

Identification


(25 samples)

P

ara

sitolo

gy

Bacte

riolo

gy

PC

R o

r R

TP

CR

OT

HE

R E

LIS

A

ELIS

A F

MD

/SP

ELIS

A F

MD

NS

P

CF

AG

ID leucosis

AG

ID P

OX

IFA

BB

AT

Pre

cip

itation (

Ascoli)

ELIS

A P

PR

MA

T (

lepto

spirosis

)

Qualit

y c

olo

rim

etr

ic tests

Rin

g T

est

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,1 1 5 3 1,5 3,4 0,05 0,01 1,13 0,1 0,017 0,035 3,5 0,01 1 0,03 8 11 1 0,01

1 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0 0,0

0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,0 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1

0,2 1,1 5,1 3,1 1,6 3,5 0,2 0,2 1,3 0,2 0,2 0,2 3,6 0,2 1,1 0,2 8,1 11,1 1,1 0,2

2.1.1. 125000 21 875

2.1.2. 0

2.1.4. 13000 3 120


2.1.5. 5200 8 528

2.1.9. 26000 3 900

2.1.13. 325 78

2.2.2. 3835 4 372

2.2.3. 3835 4 372

2.2.4. 3835 920

2.2.5. 0

2.2.6. 0

2.2.7 0

2.3.2. 0

2.3.4. 700 #REF!

2.3.9. 0

2.3.11. #REF!

2.3.12. #REF!

2.3.14. #REF!

2.4.3. 78000 780000 3265 137 835

2.4.11. 17500 2 625

2.5.3. 18330 3 483

2.5.10. #REF!

2.5.11. 18330 #REF!

2.7.11. 39000 141 960

2.7.14. 19500 #REF!

2.8.8. #REF!



other ELISA 552

6000 6 840

10000 1 116

15300 16 854

0

2000 15300 126 234

15300 123 954

15300 169 854

0

7000 35 980

41177 34235 35980 3931 8528 27612 21785,4 2625 24765 78 122460 21875 141960 3900 2280 555,05 249084 2E+05 17442 1500 932 215



Antibiotics


Pesticides

Cost by Test:


Mycology

GMO

Radiological tests

HeavyMetals

Glanders




Swine influenza

Newcastle disease


Egg Drop Syndrome

Avian influenza

Dourine



Laboratory Tests



Leptospirosis


1 421 302





Anthrax

Aujeszky's disease











Rad

iolo

gic

al T

est

Cost by

Programme:

Consumables

for National

Laboratory

(AB)

Cost by

Programme

Rabies

Other Tests

LC

-MS

(antibio

tiques,h

orm

ones,m

ycoto

xin

e)

GC

-MS

(P

esticid

es,p

esticid

es)

AA

Spectr

ophoto

metr

y

(heavyM

eta

ls)



Number of Tests

Serology

OIE

Manual C

hapte

r N

um

ber

Agent

Identification


(25 samples)


61

Annex 6: Calculation Tool North region


Buildings and Premises 3 000 000 1 0,05 150 000 6,53%

Vehicles 10 000 15 0,2 30 000 1,31%








Specialists 1 248 58 72 384 3,15%


directors 2 652 9 23 868

Support Staff 780 29 22 620 0,98%

Per diem and travel allowance in the country 1 440 1 1 440 0,06%



Continuing Education (short courses, etc.) Salaries 5% 8 928 0,39%




Equipment

20%

720 000 31,34%



350 100%

350 0,02%

Other - 0,00%

Total 2 297 145 100,00%

Renewal


Kyrgyz Republic North Region (Bishkek)9



62

566 750

178 760

789 285

1 534 795

0,44

Type of Analysis

Proposed

Standard

Relative

Value

in points

(f)

Estimated

Reagent

Cost in the

country

in €

(o)

Cost of

Sampling

Kits &

Local

Transport

in €

(l)

Total

Estimated

Reference

International

Price

in €

(m)

Number

of

Tests

(v)

Number

of

Points

(w) = v * f

Estimated

Test Cost

in €

(x) = o + l

+ (f * u)

Possible

Income

Generated

under Full

Cost

Recovery

(y) = v * x

% of

Economic

Activity

J

% of

Volume of

Activity

K


Parasitology 7,5 0,001 0,10 15,10 150 000 1 125 000 3,39 509 073 23,56% 32,18%

Bacteriology 12,5 0,001 0,10 25,10 22 000 275 000 5,59 122 959 5,69% 7,87%

PCR or RTPCR 20 5 0,10 40,10 700 14 000 13,88 9 717 0,45% 0,40%

Serology

ELISA 3 3 0,10 6,10 30 000 90 000 4,42 132 514 6,13% 2,57%

CF 3 0,05 0,10 6,10 61 800 185 400 1,47 90 669 4,20% 5,30%

AGID 2 0,01 0,10 4,10 43 000 86 000 0,99 42 488 1,97% 2,46%

IFA 5 0,1 0,10 10,10 175 875 2,40 419 0,02% 0,03%

BBAT 0,5 0,017 0,10 1,10 435 000 217 500 0,34 146 387 6,78% 6,22%

Agg (ASCOLI) 1 0,03 0,10 2,10 125 000 125 000 0,57 71 130 3,29% 3,58%

PRN/CAT 1 0,28 0,10 2,10 0 0,82 - 0,00% 0,00%

HI 3 0,83 0,10 6,10 0 2,25 - 0,00% 0,00%

MAT 10 0,01 0,10 20,10 0 4,50 - 0,00% 0,00%

Other Tests

LC-MS 42,5 7,85 0,10 85,10 15 300 650 250 26,61 407 123 18,84% 18,60%

CPG 35 10 0,10 70,10 15 300 535 500 25,47 389 637 18,03% 15,32%

Spectrophotometry 12,5 10 0,10 25,10 15 300 191 250 15,59 238 497 11,04% 5,47%


Parameters 15 11,08 0,10 30,100 17,77 - 0,00% 0,00%

Totals 913 575 3 495 775 2 160 612 100,00% 100,00%

3. Estimated Cost of Veterinary Laboratory Analysis

(u) = H6 / G36 Value of 1 Point(Excluding Reagents)

Annual Budget (Excluding Reagents & External Services)

Other Costs (Excluding Reagents & External Services)

Staff

Capital Investment


63

South region


Buildings and Premises 782 950 1 0,05 39 148 2,29%

Vehicles 10 000 13 0,2 26 000 1,52%








Specialists 1 248 62 77 376 4,52%


directors 2 652 10 26 520 1,55%

Support Staff 780 18 14 040 0,82%

Per diem and travel allowance in the country 65 1 65 0,004%



Continuing Education (short courses, etc.) Salaries 5% 7 391,60 0,43%




Equipment

20%

340 000 19,85%



1 000 100%

1 000 0,06%

Other - 0,00%

Total 1 712 493 100,00%

Renewal


Kyrgyz Republic South Region(Osh) 10



64

247 908

135 417

395 953

779 278

0,14

Type of Analysis

Proposed

Standard

Relative

Value

in points

(f)

Estimated

Reagent

Cost in the

country

in €

(o)

Cost of

Sampling

Kits &

Local

Transport

in €

(l)

Total

Estimated

Reference

International

Price

in €

(m)

Number

of

Tests

(v)

Number

of

Points

(w) = v * f

Estimated

Test Cost

in €

(x) = o + l

+ (f * u)

Possible

Income

Generated

under Full

Cost

Recovery

(y) = v * x

% of

Economic

Activity

J

% of

Volume of

Activity

K


Parasitology 7,5 0,001 0,10 15,10 188 000 1 410 000 1,13 211 666 10,43% 24,73%

Bacteriology 12,5 0,001 0,10 25,10 13 700 171 250 1,81 24 785 1,22% 3,00%

PCR or RTPCR 20 8,35 0,10 40,10 700 14 000 11,18 7 828 0,39% 0,25%

Serology

ELISA 3 3 0,10 6,10 53 000 159 000 3,51 186 028 9,17% 2,79%

CF 3 0,05 0,10 6,10 114 600 343 800 0,56 64 171 3,16% 6,03%

AGID 2 0,01 0,10 4,10 37 000 74 000 0,38 14 182 0,70% 1,30%

IFA 5 0,1 0,10 10,10 325 1 625 0,88 287 0,01% 0,03%

BBAT 0,5 0,017 0,10 1,10 780 000 390 000 0,19 144 554 7,13% 6,84%

Agg (ascoli) 1 0,03 0,10 2,10 125 000 125 000 0,27 33 331 1,64% 2,19%

MAT 10 0,01 0,10 20,10 26 000 260 000 1,48 38 389 1,89% 4,56%

Other Tests

HPLC 42,5 10 0,10 85,10 30 600 1 300 500 15,91 486 775 23,99% 22,81%

CPG 35 10 0,10 70,10 30 600 1 071 000 14,88 455 413 22,45% 18,78%

Spectrophotometry 12,5 10 0,10 25,10 30 600 382 500 11,81 361 329 17,81% 6,71%


Parameters 15 12,52 0,10 30,100 14,67 - 0,00% 0,00%

Totals 1 430 125 5 702 675 2 028 739 100,00% 100,00%

3. Estimated Cost of Veterinary Laboratory Analysis

(u) = H6 / G36 Value of 1 Point(Excluding Reagents)

Annual Budget (Excluding Reagents & External Services)

Other Costs (Excluding Reagents & External Services)

Staff

Capital Investment


65

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OIE PVS LABORATORY - Home: OIE - World Organisation for …oie.int/fileadmin/Home/eng/Support_to_OIE_Members/pdf/... · 2018-01-24 · Pathway Laboratory Team used a hypothetical