Vol. 268, No. 31 The Journal of November 5, 1993

Biological Chemistry Copyright 0 1993 by the American Society for Biochemistry and Molecular Biology, Inc.

428 East Preston St., Baltimore, MD 21202 U.S.A.

CONTENTS*

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COMMUNICATIONS

tion of the amyloid @-protein. Albert Y. Hung, Christian Activation of protein kinase C inhibits cellular produc-

Haass, Roger M. Nitsch, Wei Qiao Qiu, Martin Citron, Richard J. Wurtmnn, John H. Growdon, and Dennis J . Selkoe

phatidylinositol 3”kinase and formation of a multi- Identification of Trk binding sites for SHC and phos-

Karl-Heinz Wiesmuller, Giinther Jung, Joseph Schlessinger, meric signaling complex. Axel Oberneier, Reiner Lammers,

and Axel Ullrich

Proteolysis and lipid-facilitated translocation are dis- tinct but competitive processes that regulate secretion of apolipoprotein B in Hep G2 cells. Nobuhiro Sakata, Xujun Wu, Joseph L. Dixon, and Henry N. Ginsberg

Receptor activation of and signal generation by the lutro in/choriogonadotropin receptor. Cooperation of ASP” of the receptor and aLYS” of the hormone. Inhoe Ji, Huawei Zeng, and Tae H. J i

Peptide environment specifies conformation. Helicity of hydrophobic segments compared in aqueous, or- ganic, and membrane environments. Shun-Cheng Li and Charles M. Deber

23041 Protein kinase C-mediated bidirectional regulation of DNA synthesis, RB protein phosphorylation, and cy- clin-dependent kinases in human vascular endothelial cells. Wei Z b u , Noriko Takuwa, Mamoru Kumada, and Yoh Takuwa

23049 Evaluation of activity of putative superoxide dismutase mimics. Direct analysis by stopped-flow kinetics. Randy H. Weiss, Amy G. Flickinger, Willie J. Rivers, Medora M. Hardy, Karl W. Aston, Una S. Ryan, and Dennis P. Riley

23055 A single residue, aspartic acid 96, in the 6 opioid recep-

Haeyoung Kong, Karen Raynor, Kazuki Yasuda, Scott T. Mae, tor specifies selective high affinity agonist binding.

Philip S. Portoghese, Graeme I . Bell, and Terry Reisine

23059 Phosphorylation of the inositol trisphosphate receptor in isolated rat hepatocytes. Suresh K. Joseph and Sean V. Ryan

23066 Inhibition of vascular endothelial cell growth by acti- vin-A. Sean A. McCarthy and Roy Bicknell

23072 Differences in the regulation of protein synthesis, cy- clin B accumulation, and cellular growth in response to the inhibition of DNA synthesis in Chinese hamster ovary and HeLa 53 cells. Andrew L. Kung, Steven W. Sherwood, and Robert T. Schimke

ARTICLES 23081 Biosynthesis of the side chain of ubiquinone:trans- prenyltransferase in rat liver microsomes. Habtemichoel

The acceptor substrate specificity of porcine submax- Teclebrhan, Jerker Olsson, Ewa Swiezewska, and Gustav illary UDP-GalNAc:polypeptide N-acetylgalactosami- Dallner nyltransferase is dependent on the amino acid se- quences adjacent to serine and threonine residues. Yang 23087 Long range propagation of conformational changes in Wang, Neera Agrwal, Allen E. Eckhardt, Robert D. Stevens, integrin a d s . Xiaoping Du, Minyi Gu, John W. Weisel, and Robert L. Hill and Mark H. Ginsberg

Chandrasekaran Nagaswami, Joel S. Bennett, Ron Bowditch,

Comparison of the membrane binding kinetics of bovine prothrombin and its fragment 1. Kenneth H. Pearce, Mar- tin Hof, Barry R. Lentz, and Nancy L. Thompson

The calcium release channel of sarcoplasmic reticulum is modulated by FK-SOB-binding protein. Dissociation and reconstitution of FKBP-12 to the calcium release channel of skeletal muscle sarcoplasmic reticulum. An- thony P. Timerman, Eunice Ogunbumni, Eileen Freund, Gre- gory Wiederrecht, Andrew R. Marks, and Sidney Fleischer

NHpterminal chain length on binding of anti-digoxin Effect of heavy chain signal peptide mutations and

antibodies. Jiang Ping, Joel F. Schildbaeh, Shyh-Yu Shaw,

Michuel N. Margolies Thomas Quertermous, Jiri Nouotny, Robert Bruccoleri, and

Conformation of apolipoprotein E in lipoproteins. Sissel Lund-Katz, Karl H. Weisgraber, Robert W. Mahley, and Michuel C. Phillips

4s-Limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization,

monoterpene cyclase. Sheila M. Colby, William R. Alonso, and bacterial expression of the catalytically active

Eva J . Katahira, Douglas J . McGarvey, and Rodney Croteau

teriophage library of random peptides. The identifica- Selection of targeted biological modifiers from a bac-

tion of novel calmodulin regulatory peptides. John R. Dedman, Marcia A. Kaetzel, Hsiao Chang Chon, Deborah J. Nelson, and Gordon A. Jamieson, Jr.

23093 Retinoic acid biphasically regulates the gene expres- sion of hepatitis B virus surface antigen in human hepatoma Hep3B cells. Shih-Lan Hsu, Yu-Fen Lin, and Chen-Kung Chou

23098 High density lipoprotein conversion mediated by hu-

Hiro I . Nishida, and Toshiro Nishida man plasma phospholipid transfer protein. An-Yw Tu ,

23106 Real time measurement of nitric oxide produced ex vivo

Kikuchi, Tetsun Nagano, Hiroshi Hayakawa, Yasunobu Hirata, by luminol-HzOZ chemiluminescence method. Kazuya

and Masaaki Hirobe

23111 Inhibition of CAMP-responsive element-mediated gene transcription by cyclosporin A and FKSOB after mem- brane depolarization. Markus Schwaninger, Roland Blume, Elke Oetjen, Gundula Lux, and Willhart Knepel

23116 A polymorphism of the human &-adrenergic receptor within the fourth transmembrane domain alters ligand

A. Green, Georgette Cole, Marie Jacinto, Michael Innis, and binding and functional properties of the receptor. Stuart

Stephen B. Liggett

23122 An anion binding site that regulates the glutamate transporter of synaptic vesicles. Joachim Hartinger and Reinhurd Jahn

23128 Effect of the relative position of the UGA codon to the unique secondary structure in the fdhF mRNA on its decoding by selenocysteinyl tRNA in Eecherichia coli. Gia-Fen T. Chen, Li Fang, and Masayori Inouye

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1993) J. Biol. Chem. 268, 14543-14551, and reprints may be obtained from the editorial office.

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23132 Function of the active-site lysine in Escherichia coli 23234 The biochemical basis for the differential anti-human serine hydroxymethyltransferase. D o u g h Schzrch, Sonia immunodeficiency virus activity of two cis enantiomers Delle Fratte, Sandra Iurescia, Sebastiana Angeloccw, Roberto of 2',3'-dideoxy-3'-thiacytidine. Violetta Skalski, Contestabile, Francesco Bossa, and Verne Schzrch Chien-Neng Chang, Ginger Dutschman, and Yung-Chr Cheng

23139 Molecular characterization and outer membrane asso- ciation of a Chlamydia trachomatie protein related to the hsp70 family of proteins. Jane E. Raulston, Carolyn H. Davis, Deborah H. Schmiel, Mark W. Morgan, and Priscilla B. Wyrick

23148 Overexpression of fetal human pigment epithelium- derived factor in Escherichia coli. A functionally active neurotrophic factor. S. Patricia Becerra, Ira Palmer, Ama- lendra Kumar, Fintan Steele, Joseph Shiloach, Vicente No- tario, and Gerald J. Chader

23239 Dependence of trp repressor-operator affinity, stoichi- ometry, and apparent cooperativity on DNA sequence and size. Yuan-Ching Liu and Kathleen Shive Matthews

23250 Effect of cholesterol on the charge and structure of apolipoprotein A-I in recombinant high density lipo- protein particles. Daniel L. Sparks, W. Sean Davidson, Sissel Lund-Katz, and Michael C. Phillips

23258 Transcripts for the high and low affinity hexose trans- porters in rat myoblasts. Ling Xia, Zhuomez Lu, and Theo- dore C. Y. Lo

23157 Mitochondrial HSP60 (P1 protein) and a HSP70-like protein (mortalin) are major targets for modification 23267 Aginactin, an agonist-regulated F-actin capping activ- duringS-(1,1,2,2-tetrafluoroethyl)-~-cysteine-in- ity is associated with an Hsc70 in Dictyostelium. Robert duced nephrotoxicity. Sam A. Bruschi, Karen A. West, John J. Eddy, Roger A. Sauterer, and John S. Condeelis W. Crabb, Radhey S. Gupta, and J a m s L. Stevens 23275 Phosphorylation of serine 69 of pS6"k in activated T

cells. Julian D. Watts, Jasbinder S. Sanghera, Steven L. Pelech, and Ruedi Aebersold 23162 Cysteine mutants of light chain-2 form disulfide bonds

in skeletal muscle myosin. Vicki L. Wolff-Long, Lakshmi D. Saraswat, and Susan Lowey 23283 The effect of retinoic acid on amino acid uDtake and

23168 Interaction of non-myristoylated NADH-cytochrome b~ reductase with cytochrome bs-dimyristoylphosphati- dylcholine vesicles. Philipp Strittmatter, Jason M. Kittler, Joanne E. Coghill, and Juris Ozols

23172 Transcriptional regulation of the carbon monoxide de-

phila. Kevin R. Sowers, Tung T. Thai, and Robert P. Gunsalus hydrogenase gene (cdhA) in Methanosarcina thermo-

23179 Nucleotide binding sites on beef heart mitochondrial F1-ATPase. Cooperative interactions between sites and specificity of noncatalytic sites. Yakou M. Milgrom and Richard L. Cross

23186 A conjugate between human urokinase and saporin, a type-1 ribosome-inactivating protein, is selectively cy- totoxic to urokinase receptor-expressing cells. Ugo Cau- allaro, Antonella del Vecchw, Dough A . Lappi, and Marco R. Soria

23191 Clathrin-coated pit-mediated receptor internalization.

Jean-Pierre Paccaud, Walter Reith, Birgitta Johansson, Role of internalization signals and receptor mobility.

Karl-Eric Mapnusson. Bernard Mach. and Jean-Louis

protein synthesis by lung fibroblasts. Meir Khpsky, Alan Fine, John L. Berk, and Ronald H. Goldstein

23289 Mitochondrial voltage-dependent anion channel. Im- munochemical and immunohistochemical characteriza- tion in rat brain. Maureen W. McEnery, Ted M. Dawson, Ajay Verma, David Gurley, Marco Colombini, and Solomon H. Snyder

23297 Mastoparan stimulates exocytosis at a Caz+-independ- ent late site in stimulus-secretion coupling. Studies with the RINm6F &cell line. Mitsuhisa Komatsu, Alison M. McDermott, Susan L. Gillison, and Geoffrey W. G. Sharp

23307 Formation of a stable inactive complex of the sarco- plasmic reticulum calcium ATPase with magnesium, beryllium, and fluoride. Alexander J . Murphy and Richard J . Coll

23311 Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. Edward R. LaVallie, Alnawaz Rehemtulla, Lisa A. Racie, Elizabeth A. DiBlusio, Cathy Ferenz, Kathleen L. Grant, Albert Light, and John M. McCoy -

Carpentier

23197 Deconjugation of bilirubin-IXa glucuronides: A physi- ologic role of hepatic microsomal &glucuronidase. James F. Whiting, Julianne P. Narciso, Verne Chapman, Ber- nard J . Rand , Richard T. Swank, and John L. Gollan

23318 Protein-tyrosine kinase ~72'"' in high affinity IgE receptor signaling. Identification as a component of pp72 and association with the receptor y chain after receptor aggregation. Marc Benhamou, N ichoh J . P. Rvba, Hidetoshi Kihara, Hikaru Nishikata, and Reuben P.

23202 The carboxyl terminus of nerve growth factor is re- quired for biological activity. Catherine C. Drinkwater, Philip A. Barker, Ueli Suter, and Eric M. Shooter

23208 The multiphosphorylation domain of the phosphorylase kinase aM and aL subunits is a hotspot of differential mRNA processing and of molecular evolution. Andrea

Manfred W. Kilimann Wullrich, Christiane Hamacher, Angela Schneider, and

23215 NAD-binding site of the C3-like ADP-ribosyltransfer- ase from Clostridium limosum. Martin Jung, Ingo Just, Josef van Damme, Joel Vandekerckhove, and Klaus Aktories

23219 Calcium oscillations in pancreatic acinar cells, evoked by the cholecystokinin analogue JMV-180, depend on functional inositol 1,4,6-trisphosphate receptors. Peter Thorn and Ole H. Petersen

23222 Riboflavinuria in the rd chicken. 6'-Splice site muta- tion in the gene for riboflavin-binding protein. Ian MacLachlan, Johannes Nimpf, Harold B. White III, and Wolf- gang J . Schneider

23227 An estrogen receptor mutant exhibiting hormone-in- dependent transactivation and enhanced affinity for the estrogen response element. Hong Xing and David J . Shapiro

Siraganian

23325 Sulfite both stimulates and inhibits the yeast vacuolar H+-ATPase. Henrik Kibak, Denis Van Eeckhout, Tom Cutler, Saundra Lee Taiz, and Lincoln Taiz

23334 Activation of protein-tyrosine kinase P72"' with con-

Asahi, Takanobu Taniguchi, Eikichi Hashimoto, Tetsuya In- canavalin A in polymorphonuclear neutrophils. Momoyo

azu, Hiroyuki Maeda, and Hirohei Yamamura

23339 The effect of aspartate hydroxylation on calcium bind- ing to epidermal growth factor-like modules in coagu-

Persson, Ingrid Dahlquist, Torbjarn Drakenberg, J o h n Sten- lation factors IX and X. Maria Selander Sunnerhagen, Egon

flo, Mark Mayhew, Michel Robin, Penny Handford, Jefferson W. Tilley, Iain D. Campbell, and George G. Brownlee

23345 The heat stable antigen (mouse CD24) gene is differ- entially regulated but has a housekeeping promoter. Roland H. Wenger, Julie M. Rochelle, Michael F. Seldin, Georges Kohler, and Peter J . Nielsen

23353 Isolation and structural characterization of monomeric and trimeric photosystem I complexes (P700.FIJFB and P 7 0 0 . F ~ ) from the cyanobacterium Synechocystis PCC 6803. Jochen Kruip, Egbert J . Boekemn, Dirk Bald, Arlen F. Boonstra, and Matthias Rogner

428 East Preston Street, Baltimore, MD 21202, U.S.A. Volume 269 for 1994: Institutions: United States, $790; Foreign, $980. Students/Postdoctoral The Journal of Biological Chemistry (ISSN 0021-9258) is published weekly by the American Society for Biochemistry and Molecular Biology, Inc.,

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to The Journal of Biological Chemistry at 428 East Preston Street, Baltimore, MD 21202, U.S.A. 130369721. Second class postage paid a t Baltimore, MD 21202, U.S.A. and at additional mailing offices. Printed in the U.S.A. Send address changes

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Genetic basis of total kininogen deficiency in Williams’ trait. Peter P. Cheung, Satya P. Kunupuli, Cheryl F. Scott, Yanina T. Wachtfogel, and Robert W. Colman

Pit-I are both necessary to mediate hormonal induction An AP-1-like factor and the pituitary-specific factor

John H. McChkey, Donald L. Bodenner, and Bruce D. of human thyrotropin gene expression. Myung K. Kim,

Weintraub

Sequence and regulation of the uapA gene encoding a uric acid-xanthine permease in the fungus Aspergillus nidulans. Lisette Gorfinkiel, George Diallim, and Claudio Scazzocchw

gene encoding urate oxidase in Aspergillus nidulans. Sequence, regulation, and mutational analysis of the

Nathalie Oestreicher and Claudin Scazzocchio

Brn-6 is a divergent POU domain factor highly ex- pressed in layer IV of the neocortex. Bogi Andersen, Marcus D. Schonemann, Richard V. Pearse II, Keith Jenne, Je f f Sugarman, and Michael G. Rosenfeld

ulates the expression of the human transferrin pro- A different combination of transcription factors mod-

moter in liver and Sertoli cells. Evelyne Schaeffer, Florian Guillou, Dominique Part, and Marw M. Zakin

Recovery of transcriptionally active chromatin restric- tion fragments by binding to organomercurial-agarose magnetic beads. A rapid and sensitive method for mon- itoring changes in higher order chromatin structure during gene activation and repression. Thelma A. Chen-Clehnd, Lidia C. Boffa, Elisabetta M. Carpaneto, Maria R. Mariani, Emmanuel Valentin, Elizabeth Mendez, and Vin- cent G. Allfrey

endothelin-mediated protein kinase C activation and Identification of the receptor subtype responsible for

atrial natriuretic factor secretion from atrial myocytes. Christopher E. Irons, Susan F. Murray, and Christopher C. Glembotski

positively linked to adenylate cyclase. Jonathan A. Bard, Cloning of a novel human serotonin receptor (S-HT?)

John Zgombick, Nika Adham, Pierre Vaysse, Theresa A. Bran- chek, and Richard L. Weinshank

The replication fate of R- and S-styrene oxide adducts on adenine N6 is dependent on both the chirality of the lesion and the local sequence context. Gary J . Lutham, Liang Zhou, Constance M. Harris, Thomas M. Harris, and R. Stephen Lloyd

growth factor-I receptor tyrosine kinase. Mette GrBn- Structure-function relationship of the insulin-like

and Steen Gammeltoft borg, Birgitte S. Wulff, Jesper S. Rasmussen, Thomas Kjeldsen,

Ethanol differentially increases a*-adrenergic and muscarinic acetylcholine receptor gene expression in NGlO8-16 cells. Guang Hu, Lisa A. Querirnit, Lou Ann Downing, and Michael E. Charness

Demonstration of an inducible cyclooxygenase in hu- man endothelial cells using antibodies raised against the carboxyl-terminal region of the cyclooxygenase-2. A d a Habib, Christophe Cremrnon, Yvelrne Frobert, Jacques Grassi, Philippe Pradelles, and JacqMs Maclouf

Intramolecular fusion of Na pump subunits assures ex- clusive assembly of the fused a and @ subunit domains into a functional enzyme in cells also expressing endog- enous Na pump subunits. Mark C. Emerick and Dough M. Fambrough

v-Src activates the expression of 92-kDa type IV col- lagenase gene through the AP-1 site and the GT box homologous to retinoblastoma control elements. A mechanism regulating gene expression independent of that by inflammatory cytokines. Hiroshi Sato, Megumi Kita, and Motoharu Seiki

Aldosterone-mediated Na/K-ATPase expression is a1

Paul A. Welling, Michael Caplan, Michael Sutters, and Gerhard isoform specific in the renal cortical collecting duct.

Giebisch

Two modes of transcription initiation in vitro at the r rnB P1 promoter of Eecherichia coli. Sergei Borukhov, Valery Sagitov, Cathleen A. Josaitis, Richard L. Gourse, and Alex Goldfarb

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Reciprocal regulation of mRNA and protein for sub- units of CAMP-dependent protein kinase (RIa and Ca) by CAMP in a neoplastic B cell line (Reh). Kjetil Taskin, Kristin Brevik Anderson, Bjorn Steen Skilhegg, Kristin Austlid Taskin, Vidar Hansson, Tore Jahnsen, and Heidi Kiil Blomhoff

Polypeptide import and degradation by isolated lyso- somes. Stanley R. Terlecky and J . Fred Dice

Isolation of two distinct 3-hydroxysteroid sulfotrans-

hydroxy versus 319-hydroxy stereospecificity. William ferases from the guinea pig adrenal. Evidence for 3a-

J. Driscoll, Brian M. Martin, Hao-Chia Chen, and Charles A. Strott

cDNA cloning of chick cartilage chondroitin sulfate

codon in the aggrecan gene associated with the chon- (aggrecan) core protein and identification of a stop

drodystrophy, nanomelia. Hao Li, Nancy B. Schwartz, and Barbara M. Vertel

Brain proline-directed protein kinase phosphorylates tau on sites that are abnormally phosphorylated in tau associated with Alzheimer’s paired helical filaments. Hemnnt K. Paudel, John Lew, Zenobia Ali, and Jerry H. Wang

Reconstitution of recombinant IO-kDa subunit of the clathrin-coated vesicle H+-ATPase. Sheng-Bin Peng, Dennis K. Stone, and Xiao-Song Xie

Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. Kunihiku Sakumi, Masato Furuichi, Teru- hisa Tsuzuki, Tetsuya Kakuma, Shun-ichiro Kawabata, Hisaji Maki, and Mutsuo Sekiguchi

Hyperphosphorylation of keratins by treatment with okadaic acid of BALB/MK-2 mouse keratinocytes. Kohji Kasahara, Tonja Kartasova, X&-qing Ren, Togo Ikuta, Ka- zuhiro Chida, and Toshw Kuroki

Repression of c-fos gene expression by thyroid hormone and retinoic acid receptors. Paloma Perez, Axel ScMnthal, and Ana Aranda

Activator region analysis of the human D l ~ dopamine

Mural Mouradian receptor gene. Mari T. Minowa, Takushi Minowa, and M.

Amino-terminal assembly of human P-glycoprotein at the endoplasmic reticulum is directed by cooperative actions of two internal sequences. William R. Sknch and Vishwanath R. Lingappa

Naturally occurring anti-band 3 antibodies bind to pro-

Lutz, Omar Gianora, Mena Nater, Erich Schweizer, and Pia tein rather than to carbohydrate on band 3. Hans U.

Stammler

Kinetics of deoxyribonucleotide insertion and exten- sion at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase. Hong Cui, Linda B. Bloom, Ramon Eritja, and Myron F. Goodman

A phenylalanine codon deletion at the UGTl gene com- plex locus of a Crigler-Najjar Type I patient generates

Joseph K. Ritter, Matthew T. Yeatman, Charlotte Kaiser, a pH-sensitive bilirubin UDP-glucuronosyltransferase.

Bruno Gridelli, and Ida S. Owens

Calcium-dependent activation of protein kinase C. The role of the C2 domain in divalent cation selectivity. Jian-Hua L m and I. Bernard Weinstein

Nuclease activities of Moloney murine leukemia virus

specificities. Stacy W. Blain and Stephen P. Goff reverse transcriptase. Mutants with altered substrate

Calpain activity increases in hepatocytes following ad- dition of ATP. Demonstration by a novel fluorescent approach. Barry G. Rosser, Stephen P. Powers, and Gregory J . Gores

Imaging of inositol 1,4,6-trisphosphate-induced Caz+ fluxes in single permeabilized hepatocytes. Demonstra- tion of both quantal and nonquantal patterns of CaZ+ release. Dominique C. Renard-Roomy, Gyorgy Hajndczky, Michele B. Seitz, Timothy G. Schneider, and Andrew P. Thomas A site on transducin a-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhib- itory subunit. Nikolai 0. Artemyeu, John S. Mills, Kelly R.

Hamm Thornburg, Daniel R. Knapp, Keuin L. Schey, and Heidi E.

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23616 Antichymotrypsin interaction with chymotrypsin. Par- titioning of the complex. Barry s. Cooperman, Elenu Stauridi, Elliott Nickbarg, Eric Rescorla, Norman M. Schech- ter, and Haruey Rubin

23626 Reaction of human chymase with reactive site variants of al-antichymotrypsin. Modulation of inhibitor versus substrate properties. Norman M. Schechter, Linda M. Jor-

Haruey Rubin dun, April M. James, Barry S. Cooperman, Zhi mei Wang, and

23634 Sequence specificity in recognition of the epidermal growth factor receptor by protein tyrosine phosphatase

J. McNamara, Ellen M. Dobrusin, Derek MacLean, Andrea 1B. Kim L. Milorski, Guochang Zhu, Cathryn G. Pearl, Dennis

Thieme-Sefler, Z@ng-Yin Zhang, Toni Sawyer, Stuart J . Decker, Jack E. Duon, and Alan R. Saltiel

23640 Overexpression of YAP2, coding for a new YAP pro- tein, and YAP1 in Saccharomyces cereuisiae alleviates

Bossier, Lisete Fernundes, Dominique Rocha, and Claudinu growth inhibition caused by 1,lO-phenanthroline. Peter

Rodrigues-Pousada

23646 Functional domain in an arginine-rich carboxyl-ter-

McCormick, Jan Renee, Keuin G. Leidal, and Robert A. Clark mind region of p47phor. William M. Nauseef, Sally

23652 Hormone response complex in a novel abscisic acid and cycloheximide-inducible barley gene. Qingxi Shen, Scott J . Uknes, and Tuan-Hua David Ho

23661 ATP-dependent translocation of ricin across the mem- brane of purified endosomes. Bruno Beaumelle, Meriem Alami, and Colin R. Hopkins

23670 Dinitrogenase reductase- and MgATP-dependent mat- uration of apodinitrogenase from Azotobacter vinelan- dii. Ronda M. Allen, Mary J. Homer, Ranjini Chatterjee, Paul W . Ludden, Gary P. Roberts, and V imd K . Shah

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Structural diversity in the a243-linked polysialic acid chains in salmonid fish egg glycoproteins. Occurrence of poly(NeuSAc), poly(NeuSGc), poly(NeuSAc, NeuSGc), poly(KDN), and their partially acetylated forms. Chihiro Sato, Ken Kitajima, Ichiro Tazawa, Yasuo Inoue, Sadako h u e , and Frederic A. Troy II Characterization of the structural requirements for as- sembly and nucleotide binding of an ATP-binding cas- sette transporter. The maltose transport system of Escherichia coli. Cynthia H. Panngiotidis, Moraima Reyes, Amanda Sieuertsen, Winfried Boos, and Howard A. Shuman Complex effects of phenylarsine oxide in T cells. Induc- tion of tyrosine phoshorylation and calcium mobiliza- tion independent of CD4S expression. Mary C. Fletcher, Lawrence E. Sumelson, and Carl H. June

Nicotine increases expression of tyrosine hydroxylase gene. Involvement of protein kinase A-mediated path- way. Bhargaua Hiremagalur, Bistra Nankoua, James Nita- ham, Richard Zeman, and Esther L. Sabban

Transcytosis of placental alkaline phosphatase-poly- meric immunoglobulin rece tor fusion proteins is reg- ulated by mutations of Serg4. Gerard Apodaca and Keith E. Mostou I n vivo and in vitro phosphorylation of the T lympho- cyte type n (Kv1.3) potassium channel. Yun-Cai Cai and James Doughs

The highest levels of purine catabolic enzymes in mice are present in the proximal small intestine. Khl id A . Mohamedali, Oiuin M. Guicherit, Rodney E. Kellems, and Frederick B. Rudolph

ADDITIONS AND CORRECTIONS Novel, non-crinophagic, degradation of connecting peptide in transformed pancreatic beta cells. Vol. 268 (1993) 16248-16252. Marguerite Neerman-Arbez and Philippe A. Halban

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Vol. 268, No. 31 The Journal of Biological Chemistry November 5, 1993

CONTENTS Arrange : Categories

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MINIREVIEW

Alan Sachs and Elmar Wahle Poly(A) tail metabolism and function in eucaryotes.

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

4s-Limonene synthase from the oil glands of spearmint (Mentha spicatu). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase. Sheila M. Colby, William R. Alonso, Eva J . Katahira, Douglas J . McGaruey, and Rodney Croteau

Biosynthesis of the side chain of ubiquinone:trans- prenyltransferase in rat liver microsomes. Habtemichael Teclebrhan, Jerker Olsson, Ewa Swiezewska, and Gustav DaUner

High density lipoprotein conversion mediated by hu- man plasma phospholipid transfer protein. An- Yue Tu, Hiro I. Nishida, and Toshiro Nishida

chains in salmonid fish egg glycoproteins. Occurrence Structural diversity in the a243-linked polysialic acid

of poly(NeuSAc), poly(NeuSGc), poly(NeuSAc, NeuSGc), ,poly(KDN), and their partially acetylated forms. Chthzro Sato, Ken Kitajima, Ichiro Tazawa, Yasuo Inoue, Sadako Inoue, and Frederic A. Troy II

CELL BIOLOGY AND METABOLISM Activation of protein kinase C inhibits cellular produc- tion of the amyloid &protein. Albert Y. Hung, Christian

J . Wurtmnn, John H. Growdon, and Dennis J. Selkoe Haass, Roger M. Nitsch, Wei Qiao Qiu, Martin Citron, Richard

Identification of Trk binding sites for SHC and phos- phatidylinositol 3"kinase and formation of a multi-

Karl-Heinz Wiesmiiller, Giinther Jung, Joseph Schlessrnger, meric signaling complex. Axel Obermeier, Reiner Lampers,

and Axel Ullrich

tinct but competitive processes that regulate secretion Proteolysis and lipid-facilitated translocation are dis-

of apolipoprotein B in Hep G2 cells. Nobuhiro Sakata, Xujun Wu, Joseph L. Dixon, and Henry N. Ginsberg

teriophage library of random peptides. The identifica- Selection of targeted biological modifiers from a bac-

Dedmnn, Marcia A. Kaetzel, Hsiao Chang Chan, Deborah J . tion of novel calmodulin regulatory peptides. John R.

Nelson, and Gordon A. Jamieson, Jr.

Protein kinase C-mediated bidirectional regulation of DNA synthesis, RB protein phosphorylation, and cy- clin-dependent kinases in human vascular endothelial cells. Wei Zhou, Noriko Takuwa, Mamoru Kumada, and Yoh Takuwa

Phosphorylation of the inositol trisphosphate receptor in isolated rat hepatocytes. Suresh K. Joseph and Sean V. Ryan

Inhibition of vascular endothelial cell growth by acti- vin-A. Sean A. McCarthy and Roy Bicknell

Differences in the regulation of protein synthesis, cy- clin B accumulation, and cellular growth in response to the inhibition of DNA synthesis in Chinese hamster ovary and HeLa 53 cells. Andrew L. Kung, Steven W. Sherwood, and Robert T. Schimke

by luminol-HIOl chemiluminescence method. Kazuya Real time measurement of nitric oxide produced ex vivo

Kikuchi, Tetsuo,Nagam, Hiroshi Hayakawq Yasumbu Hirata, and Masaakr Hrrobe

!d by Subjecl

23197

23219

23234

23267

23275

23283

23297

23318

23334

23376

23417

23435

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23460

Deconjugation of bilirubin-IXa glucuronides: A physi- ologic role of hepatic microsomal j3-glucuronidase. James F. Whiting, Julianne P. Narciso, Verne Chapmnn, Ber- nard J. Ransil, Richard T. Swank, and John L. Gollan

Calcium oscillations in pancreatic acinar cells, evoked by the cholecystokinin analogue JMV-180, depend on functional inositol 1,4,6-trisphosphate receptors. Peter Thorn and Ole H. Petersen

The biochemical basis for the differential anti-human immunodeficiency virus activity of two cis enantiomers of 2',3'-dideoxy-3'-thiacytidine. Violetta Skalski, Chien-Neng Chang, Ginger Dutschman, and Yung-Chi Cheng

Aginactin, an agonist-regulated F-actin capping activ- ity is associated with an Hsc70 in Dictyoetelium. Robert J . Eddy, Roger A. Sauterer, and John S. Condeelis

cells. Julian D. Watts, Jasbinder S. Sanghera, Steven L. Phosphorylation of serine 69 of p66"* in activated T

Pelech, and Ruedi Aebersold

The effect of retinoic acid on amino acid uptake and

Fine, John L. Berk, and Ronald H. Goldstein protein synthesis by lung fibroblasts. Meir Krupsky, Alan

Mastoparan stimulates exocytosis a t a Ca2+-independ- ent late site in stimulus-secretion coupling. Studies with the RINmSF &cell line. Mitsuhisa Komntsu, Alison M. McDermott, Susan L. Gillison, and Geoffrey W. G. Sharp

Protein-tyrosine kinase ~ 7 2 " ~ ' in high affinity IgE receptor signaling. Identification as a component of pp72 and association with the receptor y chain after receptor aggregation. Marc Benhamou, Nicholas J . P. Ryba, Hidetoshi Kihara, Hikaru Nishikata, and Reuben P. Siraganian

Activation of protein-tyrosine kinase P72.yk with con-

Asahi, Takanobu Taniguchi, Eikichi Hashimoto, Tetsuya In- canavalin A in polymorphonuclear neutrophils. Momoyo

azu, Hiroyuki Maeda, and Hirohei Yamamura

uric acid-xanthine permease in the fungus Aspergillus Sequence and regulation of the uapA gene encoding a

nidulans. Lisette Gorfinkiel, George Diallinus, and Claudio Scazzocchio

endothelin-mediated protein kinase C activation and Identification of the receptor subtype responsible for

atrial natriuretic factor secretion from atrial myocytes. Christopher E. Irons, Susan F. Murray, and Christopher C. Glernbotski

Structure-function relationship of the insulin-like growth factor-I receptor tyrosine kinase. Mette Gr0n- borg, Birgitte S. Wulff, Jesper S. Rasmussen, Thomas Kjeldsen, and Steen Gammeltoft

Demonstration of an inducible cyclooxygenase in hu- man endothelial cells using antibodies raised against

A d a Habib, Christophe Criminon, Yueline Frobert, Jacques the carboxyl-terminal region of the cyclooxygenase-2.

Grassi, Philippe Pradelles, and Jacques Maclouf

Intramolecular fusion of Na pump subunits assures ex- clusive assembly of the fused a and 19 subunit domains into a functional enzyme in cells also expressing endog-

Fambrough enous Na pump subunits. Mark C. Emerick and Douglas M.

v-Src activates the expression of 92-kDa type IV col- lagenase gene through the AP-1 site and the GT box homologous to retinoblastoma control elements. A

that by inflammatory cytokines. Hrroshr Sato, Megumi mechanism regulating gene expression independent of

Kita, and Motoharu Seiki 23186 A conjugate between human urokinase and saporin, a

type-1 ribosome-inactivating protein, is selectively cy- 23469 Aldosterone-mediated Nam-ATPase expression is a1 totoxic to urokinase receptor-expressing cells. Ugo Cau- isoform specific in the renal cortical collecting duct. allaro, Antonella del Vecchio, Douglas A. Lappi, and Marc0 R. Soria

Paul A. Welling, Michael Caplan, Michael Sutters, and Gerhard Giebisch

Indicates a Communication

V l l l ...

ix

23483

23490

23496

23531

23538

23593

23601

23634

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23661

23697

23704

23712

23728

23734

22979

23132

23208

Reciprocal regulation of mRNA and protein for sub-

by CAMP in a neoplastic B cell line (Reh). Kjetil Taskin, units of CAMP-dependent protein kinase (RIa and Ca)

Austlid Taskin, Vidar Hansson, Tore Jahnsen, and Heidi Kristin Brevik Andersson, Bj0m Steen Smhegg, Kristin

Kiil Blomhoff

Polypeptide import and degradation by isolated lyso- somes. Stanley R. Terlecky and J. Fred Dice

Isolation of two distinct 3-hydroxysteroid sulfotrans-

hydroxy versus 3b-hydroxy stereospecificity. William fer- from the guinea pig adrenal. Evidence for 3a-

J. Driscoll, Brian M. Martin, Hao-Chia Chen, and Charles A. Strott

Hyperphmphorylation of keratins by treatment with okadaic acid of BALB/MK-2 mouse keratinocytes. Kohji Kasahara, Tonja Kartasova, Xiao-qing Ren, Togo Ikuta, Ka- zuhiro Chida, and Toshw Kuroki

Repression of c-foe gene expression by thyroid hormone and retinoic acid receptors. Paloma Perez, Axel Schonthal, and Ana Amnda

Calpain activity increases in hepatocytes following ad- dition of ATP. Demonstration by a novel fluorescent

J . Gores approach. Barry G. Rosser, Stephen P. Powers, and Gregory

Imaging of inositol 1,4,6-trisphosphate-induced Cas' tion of both quantal and nonquantal patterns of Cas+ fluxes in single permeabilized hepatocytes. Demonstra-

Michele B. Seitz, Timothy G. Schneider, and Andrew P. release. Dominique C. Renard-Rooney, Gyorgy H a j k z k y ,

ThomaS Sequence specificity in recognition of the epidermal growth factor receptor by protein tyrosine phosphatase

J . McNamara, EUen M. Dobrusin, Derek MacLean, Andrea 1B. Kim L. Milarski, Guochang Zhu, Cathryn G. Pearl, Dennis

Decker, Jack E. Dizon, and Alan R. Saltiel Thieme-Sefler, Zhong-Yin Zhang, Tomi Sawyer, Stuart J.

Functional domain in an arginine-rich carboxyl-ter-

McCormick, Jan Renee, Kevin G. Leidal, and Robert A. Clark minal region of p47phox. William M. Nauseef, Sally

brane of purified endosomes. Bruno Beaumelle, Miriem ATP-dependent translocation of ricin acrosa the mem-

Alami, and Colin R. Hopkins

Complex effects of phenylarsine oxide in T cells. Induc-

tion independent of CD46 expression. Mary C. Fletcher, tion of tyrosine phoshorylation and calcium mobiliza-

Lawrence E. Samelson, and Carl H. June

Nicotine increases expression of tyrosine hydroxylase gene. Involvement of protein kinase A-mediated path- way. Bhargava Hiremugalur, Bistra Nankova, James Nita- hara, Richard Zeman, and Esther L. Sabban

Transcytmis of placental alkaline phosphatase-poly- meric immunoglobulin rece tor fusion proteins is reg- ulated by mutations of Serg4. Gerard Apodaca and Keith E. Mostou

The highest levels of purine catabolic enzymes in mice are present in the proximal small intestine. Khalid A. Mohamedali, Oivin M. Guicherit, Rodney E. KeUems, and Frederick B. Rudolph

Novel, non-crinophagic, degradation of connecting peptide in transformed pancreatic beta cells. Vol. 268

Philippe A. Halban (1993) 16248-16262. Marguerite Neerman-Arbez and

ENZYMOLOGY The acceptor substrate specificity of porcine submax- illary UDP-GalNAc:polypeptide N-acetylgalactosami- nyltransferase is dependent on the amino acid se- quences adjacent to serine and threonine residues. Yang Wang, Neera Agrwal, Allen E. Eckhardt, Robert D. Steuens, and Robert L. Hill

Function of the active-site lysine in Escherichia coli serine hydroxymethyltransferase. D o u g h Schirch, Sonia Delle Fratte, Sandra Iurescia, Sebastiana Angelnccio, Roberto Contestabik, Francesco Bossa, and Verne Schirch

The multiphosphorylation domain of the phosphorylase kinase UM and a~ subunits is a hotspot of differential mRNA processing and of molecular evolution. Andrea

Manfred W. Kilimann Wdlrich, Christiane H a m h e r , Angela Schneider, and

23382

23585

23616

23626

23670

22984

22992

23122

23179

23191

23258

23289

23307

23325

23353

23441

23519

23562

23685

Sequence, regulation, and mutational analysis of the gene encoding urate oxidase in AspergiUus nidulans. Nathalie Oestreicher and Claudw Scazzocchw

Nuclease activities of Moloney murine leukemia virus reverse transcriptase. Mutants with altered substrate specificities. Stacy W. Blain and Stephen P. Goff

Antichymotrypsin interaction with chymotrypsin. Par- titioning of the complex. Barry s. Cooperman, Elena Stauridi, Elliott Nic%arg, Eric Rescorla, Norman M. Schech- ter, and Harvey Rubzn

Reaction of human chymase with reactive site variants of ul-antichymotrypsin. Modulation of inhibitor VerSUS substrate properties. Norman M. Schechter, Linda M. Jor- dan, April M. James, Barry S. Cooperman, Zhi mei Wang, and Harvey Rubin

Dinitrogenase reductase- and MgATP-dependent mat- uration of apodinitrogenase from Azotobacter vinelan- dii. Ronda M. Allen, Mary J. Homer, Ranjini Chatterjee, Paul W. Ludden, Gary P. Roberts, and Vinal K. Shah

MEMBRANES AND BIOENERGETICS Comparison of the membrane binding kinetics of bovine prothrombin and its fragment 1. Kenneth H. Pearce, Mar- tin Hof, Barry R. Lentz, and Nancy L. Thompson

The calcium release channel of sarcoplasmic reticulum is modulated by FK-606-binding protein. Dissociation and reconstitution of FKBP-12 to the calcium release channel of skeletal muscle aarcoplasrnic reticulum. An- thony P. Timerman, Eunice Ogunbumni, Eileen Freund, Gre- gory Wiederrecht, Andrew R. Marks, and Sidney Fleischer

An anion binding site that regulates the glutamate transporter of synaptic vesicles. Joachim Hartinger and Reinhard Jahn

Nucleotide binding sites on beef heart mitochondrial FI-ATPase. Cooperative interactions between sites and specificity of noncatalytic sites. Yakov M. Milgrom and Richard L. Cross

Clathrin-coated pit-mediated receptor internalization.

Jean-Pierre Paccaud, Walter Reith, Bigitta Johansson, Role of internalization signals and receptor mobility.

Karl-Eric Magnuason, Bernard Mach, and Jean-Louis Carpentier

Transcripts for the high and low affinity hexose trans- porters in rat myoblasts. Ling Xia, Zhwmei Lu, and Theo- dore C. Y. Lo

Mitochondrial voltage-dependent anion channel. Im- munochemical and immunohistochemical characteriza-

Ajay Verma, David Gurley, Marc0 Colombini, and Solomon H. tion in rat brain. Maureen W. McEnery, Ted M. Dawson,

Snyder

plasmic reticulum calcium ATPase with magnesium, Formation of a stable inactive complex of the sarco-

beryllium, and fluoride. Alexander J. Murphy and Richard J . Coll

€I+-ATPase. Henrik Kibak, Denis Van Eeckhout, Tom Cutler, Sulfite both stimulates and inhibits the yeast vacuolar

Saundra Lee Taiz, and Lincoln Taiz

Isolation and structural characterization of monomeric and trimeric photosystem I complexes (P700. FJFe and P 7 0 0 . F ~ ) from the cyanobacterium Synechocyetie PCC 6803. Jochen Kruip, Egbert J. Boekema, Dirk Bald, Arlen F. Boonstra, and Mat thm Rogner

Ethanol differentially increases an-adrenergic and muscarinic acetylcholine receptor gene expression in NGlO8-16 cells. Guang Hu, Lisa A. Querimit, Lou Ann Downing, and Michnel E. Charness

Reconstitution of recombinant 40-kDa subunit of the clathrin-coated vesicle H+-ATPase. Sheng-Bin Peng, Dennis K. Stone, and Xiao-Song Xie

Naturally occurring anti-band 3 antibodies bind to pro-

Lutz, Omar Gianora, Mena Nater, Erich Schweizer, and Pia tein rather than to carbohydrate on band 3. Hans U. Stammler

Characterization of the structural requirements for as- sembly and nucleotide binding of an ATP-binding cas- sette transporter. The maltose transport system of

Amanda Sievertsen, Winfried Boos, and Howard A. Shuman Escherichia coli. Cynthia H. Panugwtidis, Moraima Reyes,

X

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

23093 Retinoic acid biphasically regulates the gene expres- sion of hepatitis B virus surface antigen in human hepatoma Hep3B cells. Shih-Lan Hsu, Yu-Fen Lin, and Chen-Kung Chou

23111 Inhibition of CAMP-responsive element-mediated gene

brane depolarization. Markus Schwaninger, Roland Blume, transcription by cyclosporin A and FK6Of3 after mem-

Elke Oetjen, Gundula Lux, and Willhart Knepel

23128 Effect of the relative position of the UGA codon to the unique secondary structure in the fdhF mRNA on its decoding by selenocysteinyl tRNA in Escherichia coli. Gia-Fen T. Chen, Li Fang, and Masayori Irwuye

23148 Overexpression of fetal human pigment epithelium- derived factor in Escherichia coli. A functionally active neurotrophic factor. S. Patricia Becerra, Ira Palmer, A m - lendra Kumar, Fintan Steele, Joseph Shiloach, Vicente No- tario, and Gerald J . Chuder

23172 Transcriptional regulation of the carbon monoxide de- hydrogenase gene (CdhA) in Methanosarcina thermo- phila. Kevin R. Sowers, Tung T. Thai, and Robert P. Gunsalus

23222 Riboflavinuria in the rd chicken. 6'-Splice site muta-

MacLachlan, Johannes Nimpf, Harold B. White III, and Wolf- tion in the gene for riboflavin-binding protein. Ian

gang J. Schneider

23227 An estrogen receptor mutant exhibiting hormone-in- dependent transactivation and enhanced affinity for the estrogen response element. Hong Xing and David J. Shapiro

23311 Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. Edward R. LaVallie, Alnawaz Rehemtulln, Lisa A. Racie, Elizabeth A.

John M. McCoy DiBhio, Cathy Ferenz, Kathleen L. Grant, Albert Light, and

23345 The heat stable antigen (mouse CD24) gene is differ- entially regulated but has a housekeeping promoter. Rolnnd H. Wenger, Julie M. Rochelle, Michael F. Seldin, Georges Kohler, and Peter J. Nielsen

23361 Genetic basis of total kininogen deficiency in Williams' trait. Peter P. Cheung, Satya P. Kunapuli, Cheryl F. Scott, Yanina T. Wachtfogel, and Robert W. Colman

23366 An AP-1-like factor and the pituitary-specific factor Pit-1 are both necessary to mediate hormonal induction of human thyrotropin B gene expression. Myung K. Krm, John H. McCloskey, Donald L. Bodenner, and Bruce D. Weintraub

23390 Brn-6 is a divergent POU domain factor highly ex-

Marcus D. Schonemann, Richard V. Pearse II, Keith Jenne, pressed in layer IV of the neocortex. Bogi Andersen,

Jeff Sugarman, and Michael G. Rosenfeld

23399 A different combination of transcription factors mod- ulates the expression of the human transferrin pro- moter in liver and Sertoli cells. Evelyne Schaeffer, Florian Guillou, Dominique Part, and Mario M. Zakin

23409 Recovery of transcriptionally active chromatin restric- tion fragments by binding to organomercurial-agarose magnetic beads. A rapid and sensitive method for mon- itoring changes in higher order chromatin structure during gene activation and repression. Thelma A.

R. Marranc, Emmanuel Valentrn, Elrzabeth Mendez, and Vrn- Chen-Cleland, Lidia C. Boffa, Elisabftta M. Carpaneto, Maria

cent G. Allfrey

23422 Cloning of a novel human serotonin receptor (S-HT?) positively linked to adenylate cyclase. Jonathan A. Bard, John Zgombick, Niku Adham, Pierre Vaysse, Theresa A. Bran- chek, and Richard L. Weinshank

23427 The replication fate of R- and S-styrene oxide adducts on adenine is dependent on both the chirality of the lesion and the local sequence context. Gary J . Lutham, Liang Zhou, Constance M. Harris, Thomas M. Harris, and R. Stephen Lloyd

23477 Two modes of transcription initiation in vitro at the r rnB P1 promoter of Escherichia coli. Serger Borukhov,

Alex Goldfarb Valery Sagitou, Cathleen A. Josaitis, Rlchard L. Gourse, and

23504

23524

23544

23567

23573

23580

23640

23652

022971

022975

23000

23008

23031

23049

23055

23087

23116

23139

cDNA cloning of chick cartilage chondroitin sulfate

codon in the aggrecan gene associated with the chon- (aggrecan) core protein and identification of a stop

Barbara M. Vertel drodystrophy, nanomelia. Ha0 Li, Nancy B. Schwartz, and

Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. Kunihiko Sakumi, Masato Furuichi, Teru- hisa Tsuzuki, Tetsuya Kakumn, Shun-ichiro Kawabata, Hisaji Maki, and Mutsuo Sekiguchi Activator region analysis of the human DIA dopamine receptor gene. Mari T. Mirwwa, Takashi Minowa, and M. Maral Mouradian

sion at abasic template lesions in different sequence Kinetics of deoxyribonucleotide insertion and exten-

Linda B. Bloom, Ramon Eritja, and Myron F. Goodman contexts using HIV-1 reverse transcriptase. Hong Cai,

plex locus of a Crigler-Najjar Type I patient generates A phenylalanine codon deletion at the UGTl gene com-

a pH-sensitive bilirubin UDP-glucuronosyltransferase. Joseph K. Ritter, Matthew T. Yeatmun, Charlotte Kaiser, Bruno Gridelli, and Ida S. Owens Calcium-dependent activation of protein kinase C. The role of the C2 domain in divalent cation selectivity. Jian-Hua Luo and I. Bernard Weinstern

tein, and YAP1 in Saccharomyces cereuisiae alleviates Overexpression of YAP2, coding for a new YAP pro-

growth inhibition caused by 1,lO-phenanthroline. Pe!er Bossier, Lisete Fernandes, Dominique Rocha, and Claudrna Rodrigues-Pousada

cycloheximide-inducible barley gene. Qingxi Shen, Scott Hormone response complex in a novel abscisic acid and

J. Uknes, and Tuan-Hua David Ho

PROTEIN CHEMISTRY AND STRUCTURE Receptor activation of and signal generation by the lutro in/choriogonadotropin receptor. Cooperation of

Inhae Ji, Huawei Zeng, and Tae H. J i ASPg7 of the receptor and aLYS" of the hormone.

Peptide environment specifies conformation. Helicity of hydrophobic segments compared in aqueous, or- ganic, and membrane environments. Shun-Cheng Li and Charles M. Deber

Effect of heavy chain signal peptide mutations and NH?-terminal chain length on binding of anti-digoxin antibodies. Jiang Ping, Joel F. Schildbach, Shyh-Yu Show, Thomas Quertermous, Jiri Nouotny, Robert Bruccoleri, and Michael N. Margolies

Conformation of apolipoprotein E in lipoproteins. Sissel Lund-Katz, Karl H. Weisgraber, Robert W. Mahley, and Michael C. Phdlrps

Oxygen equilibrium studies of cross-linked iron-cobalt hybrid hemoglobins. Models for partially ligated inter- mediates of cobalt hemoglobin. Antonio Tsuneshige, Yu-xiang Zhou, and Takashi Yonetani

Evaluation of activity of putative superoxide dismutase mimics. Direct analysis by stopped-flow kinetics. Randy H. Weiss, Amy G. Flickinger, WiUie J . Rivers, Medora M. Hardy, Karl W. Aston, Una S. Ryan, and Dennis P. Riley A single residue, aspartic acid 95, in the 6 opioid recep-

H w o u n g Kong, Karen Raynor, Kazuki Yasuda, Scott T. Moe, tor specifies selective high affinity agonist binding.

Phrlzp S. Portoghese, Graeme I. Bell, and Terry Rewne Long range propagation of conformational changes in integrin a d 8 . Xiaoping Du, Minyi Gu, John W. Weisel, Chandrasekuran Nagaswamr, Joel S. Bennett, Ron Bowditch, and Mark H. Ginsberg A polymorphism of the human &adrenergic receptor within the fourth transmembrane domain alters ligand

A. Green, Georgette Cole, Marie Jacinto, Michael Innis, and binding and functional properties of the receptor. Stuart

Stephen B. Liggett

Molecular characterization and outer membrane asso- ciation of a Chlamydia trachomatis protein related to the hsp70 family of proteins. Jane E. Raulston, Carolyn H. Davis, Deborah H. Schmiel, Mark W. Morgan, and Priscilla B. Wyrick

Indicates a Communication

xi

23157 Mitochondrial HSPBO (P1 protein) and a HSP7O-like protein (mortalin) are major targets for modification during S-(1,1,2,2-tetrafluoroethyl)-~-cysteine-in- duced nephrotoxicity. Sam A. Bruschi, Karen A. West, John W. Crabb, Radhey S. Gupta, and James L. Stevens

23162 Cysteine mutants of light chain-2 form disulfide bonds in skeletal muscle myosin. Vicki L. Wolff-Long, Lakshmi D. Saraswat, and Susan Lowey

23168 Interaction of non-myristoylated NADH-cytochrome b5 reductase with cytochrome b~l-dimyristoylphosphati- dylcholine vesicles. Philipp Strittmatter, Jason M. Kittler, Joanne E. Coghill, and Juris Ozols

23202 The carboxyl terminus of nerve growth factor is re-

Philip A. Barker, Ueli Suter, and Eric M. Shooter quired for biological activity. Catherine C. Drinkwater,

23215 NAD-binding site of the C3-like ADP-ribosyltransfer- ase from Clostridium limosum. Martin Jung, Zngo Just, Josef van Damme, Joel Vandekerckhue, and Klaus Aktories

23239 Dependence of trp repressor-operator affinity, stoichi- ometry, and apparent cooperativity on DNA sequence and size. Yuan-Ching Liu and Kathleen Shive Matthews

23250 Effect of cholesterol on the charge and structure of apolipoprotein A-I in recombinant high density lipo-

Lund-Katz, and Michael C. Phillips protein particles. Daniel L. Sparks, W. Sean Dauidson, Sissel

23339 The effect of aspartate hydroxylation on calcium bind-

lation factors IX and X. Maria Selander Sunner en, Egon ing to epidermal growth factor-like modules in coagu-

f l o , Maik %ayhew, &cAl Robm, Penny H a d o r d , Jefferson Persson I rid Dah v s t , Torbjorn Drakenber J%n Sten-

W . Tilley, Iam D. Campbell, and George G. Brownlee 23512 Brain proline-directed protein kinase phosphorylates

tau on sites that are abnormally phos horylated In tau

H e m n t K . Paudel, John Lew, Zenobia All, and Jerry H. Wang associated with Alzheimer’s paired ,gelical filaments.

23552 Amino-terminal assembly of human P- lycoprotein at the endo lasmic reticulum is directed %y coo erative actions of two internal sequences. William R. &h and Vishwanath R. Lingappa

23611 A site on transducin a-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhib- itory subunit. Nikalai 0. Artemyev, John S. Mills, Kell R

Hamm Thornburg, Danrel R. Kmpp, Kevin L. Schey, and H e d E:

23720 In vivo and in uitro phosphorylation of the T 1,ympho- e n (Kv1.3) potassium channel. Yun-Cal Cat and

~ ~ 2 e B ~ o u g g l a s s

Adham, Nika, 23422 Aebersold, Ruedi, 23275 A p a l , Neera, 22979

Alami, Mbriem, 23661 Aktories, Klaus, 23215

Ah, Zenobia, 23512 Allen, Ronda M., 23670 Allfrey, Vincent G., 23409 Alonso, William R., 23016

Anderason, Kristin Brevik, Anderaen, Bod, 23390

Apodaca, Gerard, 23712 Angelaccio, Sebastiana, 23132

Aranda, Ana, 23538 Artemyev, Nikolai O., 23611 Asahi, Momoyo, 23334 Aston, Karl W., 23049

Bald, Dirk, 23353 Bard, Jonathan A., 23422 Barker, Philip A., 23202

Becerra, S. Patricia, 23148 Beaumelle, Bruno, 23661

Bell, Graeme I., 23055 Benhamou, Marc, 23318 Bennett, Joel S., 23087 Berk, John L., 23283

Blain, Stacy W., 23585 Bicknell, Roy, 23066

Blomhoff, Heidi Klil, 23483 Bloom, Linda B., 23567 Blume, Roland, 23111 Bodenner, Donald L., 23366

Boffa, Lidia C., 23409 Boekema, Egbert J., 23353

Boos, Winfried, 23685 Boonstra, Arjen F., 23353

Borukbov, Sergei, 23477 Bosen, Francesco, 23132

Bowditch, Ron, 23087 Bossier, Peter, 23640

Branchek, Theresa A., 23422 Brownlee, George G., 23339 Bruccoleri, Robert, 23000 Bruschi, Sam A., 23157

Cai, Hong, 23567 Cai, Yun-Cai, 23720 Campbell, Iain D., 23339 Caplan, Michael, 23469

23483

Carpaneto, Elisabetta M.,

Carpentier, Jean-Louis,

Cavallaro, Ugo, 23186 Chader, Gerald J., 23148 Chan, Hsiao Chang, 23025 Chang, Chien-Neng, 23234 Chapman, Verne, 23197

Chattejee, Ranjini, 23670 Cbarness, Michael E., 23441

Chen, Gia-Fen T., 23128 Chen, Hao-Chia, 23496 Chen-Cleland, Thelma A.,

Cheng, YungChi, 23234 Cheung, Peter P., 23361

Chou, Chen-Kung, 23093 Chida, Kazuhiro, 23531

Citron, Martin, 22959 Clark, Robert A., 23646

Colby, Sheila M., 23016 Coghill, Joanne E., 23168

Cole, Georgette, 23116 Coll, Richard J., 23307 Colman, Robert W., 23361 Colombini, Marco, 23289

Contestabile, Roberto, 23132 Condeelis, John S., 23267

Cooperman, Barrv S.. 23616.

23409

23191

23409

- . 23626

Crabb, John W., 23157 Cniminon, Christophe, 23448

Croteau, Rodney, 23016 Cross, Richard L., 23179

Cutler, Tom, 23325 Dahlqvist, Ingrid, 23339 Dallner, Gustav, 23081 Davidson, W. Sean, 23250 Davis, Carolyn H., 23139 Dawson, Ted M., 23289

Decker, Stuart J., 23634 Deber, Charles M., 22975

Dedman, John R., 23025 del Veccbio, Antonella, 23186 Diallinas, George, 23376 DiBlasio, Elizabeth A,, 23311 Dice, J. Fred, 23490 Dixon, Jack E., 23634

AUTHOR INDEX

Dixon, Joseph L., 22967 Dobrusin, Ellen M., 23634 Douglass, James, 23720

Drakenberg, Torbjorn, 23339 Downing, Lou Ann, 23441

Drinkwater, Catherine C.,

Driscoll, William J., 23496 Du, Xiaoping, 23087 Dutscbman, Ginger, 23234 Eckhardt, Allen E., 22979

Emerick, Mark C., 23455 Eddy, Robert J., 23267

Eritja, Ramon, 23567

23202

Fambrougb, Douglas M., 23455

Fang, Li, 23128 Ferenz, Cathy, 23311 Fernandes, Lisete, 23640 Fine, Alan, 23283 Fleiscber, Sidney, 22992 Fletcher, Mary C., 23697 Flickinger, Amy G., 23049

Freund, Eileen, 22992 Fratte, Sonia Delle, 23132

Frobert, Yveline, 23448 Furuichi, Masato, 23524 Gammeltoft, Stem, 23435 Gianora, Omar, 23562 Giebiscb, Gerhard, 23469 Gillison, Susan L., 23297

Ginsberg, Mark H., 23087 Ginsberg, Henry N., 22967

Glembotski, Christopher C.,

Goff, Stephen P., 23585 Goldfarb, Alex, 23477 Goldstein, Ronald H., 23283 Gollan, John L., 23197 Goodman, Myron F., 23567 Gores, Gregory J., 23593 Gorfinkiel, Lisette, 23376 Gourse, Richard L., 23477

Grassi, Jacques, 23448 Grant, Kathleen L., 23311

Green, Stuart A., 23116 Gridelli, Bruno, 23573 GrBnborg, Mette, 23435

23417

Growdon, John H., 22959 Gu, Minyi, 23087 Guicherit, Oivin M., 23728 Guillou, Florian, 23399 Gunsalus, Robert P., 23172 Gupta, Radhey S., 23157 Gurley, David, 23289 Haass, Christian, 22959 Habib, Aida, 23448 Hajnkzky, Gyorgy, 23601 Halban, Philippe A., 23734

Hamm, Heidi E., 23611 Hamacher, Christiane, 23208

Handford, Penny, 23339 Hansson, Vidar, 23483 Hardy, Medora M., 23049 Harris, Constance M., 23427

Hartinger, Joachim, 23122 Harris, Thomas M., 23427

Hashimoto, Eikichi, 23334 Hayakawa, Hiroshi, 23106 Hill, Robert L., 22979 Hirata, Yasunobu, 23106 Hiremagalur, Bhargava,

Hirobe, Masaaki, 23106 Ho, Tuan-Hua David, 23652

Homer, Mary J., 23670 Hof, Martin, 22984

Hsu, Shih-Lan, 23093 Hopkins, Colin R., 23661

Hu, Guang, 23441 Hung, Albert Y., 22959 Ikuta, Togo, 23531 Inazu, Tetsuya, 23334 Innis, Michael, 23116 Inoue, Sadako, 23675 Inoue, Yasuo, 23675 Inouye, Masayori, 23128 Irons, Christopher E., 23417 Iurescia, Sandra, 23132 Jacinto, Marie, 23116 Jahn, Reinhard, 23122

James, April M., 23626 Jahnsen, Tore, 23483

Jamieson, Gordon A., Jr.,

Jenne, Keith, 23390

23704

23025

Ji, lnhae, 22971 Ji, Tae H., 22971 Johansson, Birgitta, 23191

Josaitis, Cathleen A,, 23477 Jordan, Linda M., 23626

Joseph, Suresh K., 23059 June, Carl H., 23697 Jung, Giinther, 22963 Jung, Martin, 23215 Just, Ingo, 23215

Kaetzel, Marcia A,, 23025 Kaiser, Charlotte, 23573 Kakuma, Tetsuya, 23524 Kartasova, Tonja, 23531 Kasahara, Kohji, 23531 Katahira, Eva J., 23016 Kawabata, Shun-ichiro.

23524 Kellems, Rodney E., 23728 Kibak, Henrik, 23325 Kihara, Hidetosbi, 23318 Kikuchi, Kazuya, 23106 Kilimann. Manfred W..

23208 Kim, Myung K., 23366 Kita, Megumi, 23460 Kitajima, Ken, 23675 Kittler, Jason M., 23168 Kjeldsen, Thomas, 23435 Knapp, Daniel R., 23611 Knepel, Willhart, 23111 Kohler, Georges. 23345 Komatsu, Mitsuhisa, 23297 Kong, Haeyoung, 23055 Kruip, Jochen, 23353 Krupsky, Meir, 23283 Kumada, Mamoru, 23041 Kumar, Amalendra, 23148 Kunapuli, Satya P., 23361 Kung, Andrew L., 23072 Kuroki, Tosbio, 23531

Lammers, Reiner, 22963 Lappi, Douglas A,, 23186 Latham, Gary J., 23427

Leidal, Kevin G., 23646 LaVallie, Edward R., 23311

Lentz, Barry R., 22984 Lew, John, 23512 Li, Hao, 23504