Natalie Murrow and Erryn Schneider FSI 2011 at The University
of Northern Colorado
Slide 2
Hybridization Negative effects of Hybridization Sclerocactus
glaucus (Threatened) Sclerocactus parviflorus S. Glaucus ( left)
and S. parviflorus (right)
http://www.botanicgardensblog.com/tag/research-and-conservation/
http://m.eb.com/assem bly/4442
Slide 3
S. parviflorus is hybridizing with S. glaucus Closely related
Differences reflect S. parviflorus S. parviflorus
http://www.flickr.com/photos/pix- diana/3818284347/
Slide 4
Samples are gathered by the Denver botanic gardens from the
grand junction area in Colorado. S. glaucus samples being collected
http://www.nmccskiclub.org/links.php
http://www.botanicgardensblog.com/author/deprengm /
Slide 5
Samples were ground with liquid nitrogen This causes cell
disruption to expose the DNA Samples are labeled and placed in a
freezer to preserve DNA.
Slide 6
The AP1 buffer solution contains detergent. The AP2 buffer
contains chemicals that will take out the detergents The AP3 buffer
contains ethanol. The new column contains tiny silica (glass) beads
that DNA will stick to. AE solution removes the DNA from the
beads.
Slide 7
2 l is run on a gel to make sure the extraction was successful.
Electric current makes it travel through gel medium Dye visible
under UV DNA fragments are different sizes Different sizes go
different rates
Slide 8
Microsatellite library Highly variable repeating regions in DNA
E.g. GTAGTAGTAGTA... Found by mixing with chemioluminescent tags
Sequenced Separated by PCR and compared
http://www.mindupbioresearch.com/bioma rkers.html
http://en.wikipedia.org/wiki/F ile:Pcr_machine.jpg PCR machine
Slide 9
Continuing study 792 individuals in 35 distinct populations
Eleven S. glaucus populations and four S. parviflorus so far
http://www.flickr.com/photos/travelingwild /3477872759/
Slide 10
Preliminary results One isolated population of S. glaucus and
another non- isolated just south of two populations of S.
parviflorus Non-isolated population had more genetic similarity to
the S. parviflorus that to the isolated population Continued study
needed
Slide 11
Preliminary results support hypothesis Hybridization could have
negative effects on S. glaucus Further research: Compare more
microsatellites Determine extent If the findings support the
hypothesis, an action plan can be made http://www.flickr.com/ph
otos/travelingwild/2411882 759/
Slide 12
Our general sponsors, Space Connections Inc. for Natalie and
The FSI Alumni of 1979 for Erryn Our research sponsors, The Edward
Madigan Foundation for Natalie and The Kinder Morgan Foundation for
Erryn Lori Ball and FSI Our teachers, Nick, Nathan, Abby, and
Zabedah Anna Schwabe, our research mentor And our friends and
family for supporting us being here. THANK YOU
Slide 13
Text: Arnold, M. L. (1997). Natural hybridization and
evolution. New York, NY: Oxford University Press. Biology animation
library: Polymerase chain reaction [Animation]. (n.d.). Retrieved
from Cold Spring Harbor Laboratory website:
http://www.dnalc.org/resources/animations/pcr.html Hanley, N.,
Spash, C., & Walker, L. (1995). Problems in valuing the
benefits of biodiversity protection. Environmental and Resource
Economics, 5, 249- 27. Retrieved from
http://www.springerlink.com/content/u4u5366l7k126145/ Hubbell, S.
P. (2001). Chapter 1. In The unified neutral theory of biodiversity
and biogeography (p. 3). Retrieved from:
http://books.google.com/books?hl=en&lr=&id=
EIQpFBu84NoC&oi=fnd&pg=PA3&dq=biodiversity&ots=cPGNJZNyu0&sig=RX
kbt4p_ 9nwTkzReWdMlhy_5ez0#v=onepage&q&f=false Recovery
outline for the Colorado hookless cactus. (2010, April). Retrieved
from U.S. Fish and Wildlife Service website:
http://ecos.fws.gov/docs/recovery_plan/CO%20hookless%20
cactus_recovery%20outline_Apr%202010.pdf
Slide 14
Text (cont.): Schwabe, A. (2011a, June 20). Sclerocactus
glaucus hybridization. Reading presented at UNC Plant Population
Genetics Lab, Greeley, CO. Schwabe, A. (2011b, July 19). The
process of sequencing and comparing DNA. Address presented at UNC
Plant Population Genetics Lab, Greely, CO. Schwabe, A., Ramp-Neale,
J., & McGlaughlin, M. E. (2011, July). Analysis of nuclear DNA
from S. glaucus and S. parviflorus to determine the level of
directionality and hybridization between these two species. Poster
session presented at Botany 2011, St Lewis, MO. Abstract retrieved
from: 2011.botanyconference.org/engine/search/index.php?func=detail
&aid=292 Science plan for McInnis Canyons National Conservation
Area. (2010, September). Retrieved from:
http://www.blm.gov/pgdata/etc/medialib/blm/co/field_offices/MCNCA/pdf.Pa
r. 96119.File.dat/McInnis%20Canyons%20Science%20Plan.pdf
Sclerocactus parviflorus. (n.d.). Retrieved from Flora of North
America website:
http://www.efloras.org/florataxon.aspx?flora_id=1&taxon_id=24241528
8
Slide 15
Text (cont.): 2011 Colorado Native Plant Society grant
recipients. (2011). Retrieved from Colorado Native Plant Society
website: http://www.conps.org/Committees/grants.shtml Vil, M.,
Weber, E., & DAntonio, C. M. (2000, September 13). Conservation
implications of invasion by plant hybridization. Biological
Invasions, 207217. Retrieved from:
http://www.montsevila.org/otherscientificpapers/Conservation%20implication
s.pdf Wendt, T., Canela, M. B. F., de Faria, A. P. G., & Rios,
R. I. (2001, October). Reproductive biology and natural
hybridization between two endemic species of Pitcairnia. American
Journal of Botany. Abstract retrieved from:
http://www.jstor.org/pss/3558350 Pictures:
http://farm3.static.flickr.com/2456/3818284347_cb609c3926.jpg
http://www.botanicgardensblog.com/author/deprengm/
http://www.swcoloradowildflowers.com/pink%20enlarged%20photo%20pages/scl
erocactus.htm
http://farm3.static.flickr.com/2108/2411882759_676e67c26a.jpg