Microsoft PowerPoint - Protein(4) 2007.PPTMathews et al (2000) Biochemistry (3e) p.214
(Hb) (Mb) Hb
Mb Hb Mb
Mb α β α2β2
P4-2
Hb Mb Hb
Hb
Hb heme Hb Hb Hb Mb Hb Hb
Hb tense (T) relaxed (R) T R
Mb
P4-3
100
80
60
40
20
0



Mb
Hb Hb Mb
Hb
hemeheme
heme Hb His imidazole E7 His
X-ray
Lehninger Principles of Biochemistry (4e) p. 136
P4-5

Garrett & Grisham (1999) Biochemistry (2e) p.486
E7 His heme F8 His
E7 His F8 His F8 helix Hb (T form → R form)
Hb Hb Hb
Hb Mb Hb Mb
Heme
heme heme
Lehninger Principles of Biochemistry (4e) p. 855 heme
P4-6

Cartoon by I. Geis
Geis
Wikipedia hemoglobin
P4-7

1
1




P4-8
Mathews et al (2000) Biochemistry (3e) p.195


(monomer) ↓
(dimer)
α helix β sheet

P4-10

EF pocket
Glu→Val
Mathews et al (2000) Biochemistry (3e) p.236


Hb heme
( )
Pauling molecular disease
P4-11
Mathews et al (2000) Biochemistry (3e) p.239
Glu Val

Glu Val
P4-12
( ... )
P4-13

Pyruvate dehydrogenase
Lehninger Principles of Biochemistry (4e) p. 604 pyruvate dehydrogenase p. 182 actin myosin
P4-14

substrate
product

Lehninger Principles of Biochemistry (4e) p. 697
P4-15

G ar
re tt
α Helix coiled coil
α ()
Lehninger Principles of Biochemistry (4e) p. 126 keratin
P4-16

Lehninger Principles of Biochemistry (4e) p. 145