MultiChrome™ Microbiology Culture System

Sensi-Ring™ Antibiotic Sensitivity System

Case Studies Reference Library

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Sensi-Ring™Antibiotic Sensitivity System

Forward: This library is designed to help those using the Kacey DiagnosticsC&S System with real world cases in which its unique chromogenic media has been used to identify bacteria and further discover its sensitivity to various antibiotics. These images were taken directly from the field and havenot been retouched. This is a work in progress and are updating this library almost daily so please visit us often. We want to thank in particular Dr. Louis Gotthelf for his invaluable help in compiling this library and to the many hospitals and clinics using this systemfor sharing their findings with fellow colleagues.

Any use of these images in a manor not approved by Kacey Diagnostics without its written consent is strictly prohibited.

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INDEX:

Type of Colony Pages

Gram Negative

Gram Positive 4

Ear Infections 5

UTI 6-7

Skin/Wound

Mixed Colonies 8-10

3

Type: Gram Positive

Patient/Breed: “Apollo”, 6 yr old CockerCulture Source: Recurrent Ear/Skin infectionsMultiChrome Culture Results:

Gram Positive: Staph/Strep both ears

Sensitivity Results:

Notes: Because these are both GP organisms,a GP Sensi-Ring was used. This is a very interesting culture because the Staph is sensitive to many of the common drugs including Penicillin. The Streptococci, however are resistant to enrofloxicin, which is typical for Streptococci. They do not usually respond to fluoroquinolones as is demonstrated here.

GP

4

Type: Ear

Patient/Breed: “Jessie”,Culture Source: R EarMultiChrome Culture Results:

Gram Negative: E. coli Gram Positive: Enterococci

Sensitivity Results:

Notes: Mueller Hinton plate was swabbedwith both GP and GN material.

GN GP

5

Type: UTI

Patient/Breed: “Molly”, 19 yr old FelineCulture Source: UrineMultiChrome Culture Results:

Gram Negative: E-coli

Sensitivity Results:

Notes: A Mueller Hinton plate was inoculated with the sediment as well. A Kacey UTI sensi ring was placed over the growth and incubated for 24 hours.

GN

6

Type: UTI

Patient/Breed: “Peaches”, 10 yr old M/FelineCulture Source: UrineMultiChrome Culture Results:

Gram Negative: E-coli, KlebGram Positive: Enterococci

Sensitivity Results:

Notes: Only the Augmentin disk (amoxicillin/clavulanate ) and the Trimethoprim/Sulfa disk showed sensitivity to all bacteria. The Gentamycin (GM) disk showed sensitivity, but the zone of inhibition was too low to be therapeutic. .

GN GP

7

Type: Mixed Infection

Patient/Breed: “Baby”, PomeranianCulture Source: Anal GlandsMultiChrome Culture Results:

Gram Positive: EnterococciGram Negative: E-coli, Kleb

Sensitivity Results:

Notes: Antibiotic used “Ciprofloxacin

GN GP

8

Type: Mixed Infection

Patient/Breed: “Kizzy”, 3 yr old F PugCulture Source: PododermatitisMultiChrome Culture Results:

Gram Positive: Staph & Strep Gram Negative: Pseudomonas &Kleb

Sensitivity Results:

Notes:K izzy has had demodex mange with pododermatitis for at least one year. There was so much swelling and exudation on the feet that the owner put socks on the dog’s feet to prevent licking. This dog has been treated for mange and bacterial skin infection by several different vets with no effect. On presentation, the feet were weeping purulent material. A culture was taken of the pus that was squeezed out of the skin lesions after the feet were disinfected in chlorhexidine.

GN GP

9

Type: Mixed Infection

Patient/Breed: “Zoe”, MalteseCulture Source: Anal GlandsMultiChrome Culture Results:

Gram Positive: ListeriaGram Negative: E.-coli

Sensitivity Results:

Notes: Antibiotic used “Clavamox”

GN GP

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