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October 21, 1997
Ms Jennifer HuffmanWise. Dept. Natural Resources1125 N. Military Ave.Green Bay, WI 54307
Re: WET Test (9-16 October, 1997) Results - Lemberger Landfill Site
Dear Jennifer:
Enclosed find the report, and copies of laboratory bench sheets, for the second of the tworequired chronic toxicity retest batteries conducted on treated effluent (groundwater) from theLemberger Landfill Site. Reporting format is the same as that used previously.
The fathead minnow test results were officially inconclusive due to poor survival in the BranchRiver control, but toxicity negative when compared to the secondary control (laboratory water).In contrast to the original and first retest batteries, results for this battery were chronic toxicitynegative for Ceriodaphnia.
Please call if you have any questions or comments.
Sincerely,
Peter J. GlassenProject Manager
enclosurescc Mr. Douglas B. Clark, Foley & Lardner
Mr. Pablo N. Valentin, USEPA Region VMr. David Sanders, RMT
•lairRMT, INC. - Fox VALLEY
RENAISSANCE PLAZA - SUITE 2104351 WEST COLLEGE AVENUE
APPLETON,W! - 54914-3928414/830-0209 - 414/830-1996 FAX
WHOLE EFFLUENT TOXICITY (WET) TEST REPORT FORMGENERAL INFORMATION
FACILITY NAME: LEMBERGER LANDFILL
DISCHARGE DESCRIPTION: TREATED GROUNDWATER
WPDES PERMIT NO: NA
OUTFALL NO.: 001
RECEIVING WATER (RW): BRANCH RIVER
LABORATORY NAME: RMT APPLIED BIOLOGY - 5191
LAB WATER (LW): MHSW
B ABITZ ROAD - APPLETON, WISCONSIN 54915
#2 #3
. - - - . - • • . . - . . • . - . • . • • . • • • • . - • ' . - • . ' • • • . . • • : . . • . ' • • • : • ' • • • • : - . . - : - : • • . • • • :
TEST INFORMATION
DATE TEST INITIATED: 10/9/97
TESTS ARE FOR:
ZID / IWC INFORMATION:
DILUTION WATER:
DILUTION SERIES:
ACUTE - NA
__ WPDES Compliance (required by per_ #1 Retest of failure on / / __
#2 Retest of failure on / /QA Restart from / /Other:
Zone of Initial Dilution Ratio: NA
C. dubia FHM Other:D RW D RW D RWD LW D LW D LW
6.25., 12.5. 25. 50. 100%__ OTHER:
CHRONIC
nit) __ WPDES Compliance (required by permit)#1 Retest of failure on / /
X #2 Retest of failure on 9/4/97QA Restart from / /Other:
Instream Waste Concentration. 100%
C. dubia FHMEl RW El RWD LW D LW
1,3,10,30,100%12.5,25,50,75,100%
X OTHER: 6.25. 12.5. 25. 50. 100%
QA/QC CONDITIONS
ACUTE - NA
TEMPERATURES MAINTAINED DURING TEST? (25+1 "C - CHRONIC)
DISSOLVED OXYGEN >40% AND <100% SATURATION THROUGHOUT TEST?
Effluent pH MAINTAINED WITHIN 6.0 - 9.0 s.u. THROUGHOUT TEST?
REFERENCE TOXICANT TESTS WITHIN ACCEPTABLE LIMITS?
WERE TESTS CONDUCTED IN A 2.5% COj ATMOSPHERE?
COMMENTS:
D YES
D YES
D YES
D YES
D YES
D NO Lx]
D NO E3
D NO B
D NO tE]
D NO D
CHRONIC
YES D NO
YES D NO
YES D NO
YES D NO
YES [x] NO
WATER CHEMISTRY [ALL VALUES REPORTED IN MG/L, EXCEPT pH (s.u.)]
WATER TYPE
RECEIVING
WATER
EFFLUENT
LAB WATER
SAMPLE tt
1
2
3
1
2
3
#1
#2
#3
HARDNESS ALKALINITY TO'
IND
390
390
370
390
80
MEAN IND
390 286
383 308
304
304
MEAN IND
286 <1.4
305 <1.4
<1.4
<1.4
56
FAL NH3-N
MEAN
<L4
<1.4
pH (after warming)
IND MEAN
NA NA
NA NA
T.R. CHLORINE
IND MEAN
<0.01 <0.01
<0.01
<0.01
1. a:\9731RPT.DOC
ACUTE TOXICITY TEST RESULTS i-NA
ZID INFORMATION
SPECIES
FATHEAD MINNOW
AGE OF ORGANISM:
Ceriodaphnia dubla
AGE OF ORGANISM:
<24-h
D. magna
orOTHER:
(Please specify)
AGE OF ORGANISM:
ZID CONCENTRATION: % EFFLUENT
EFFLUENTTREATMENT
LWCONTROL
RWCONTROL
6.25%
12.5%
25%
50%
100%
LWCONTROL
RWCONTROL
6.25%
12.5 %
25%
50%
100%
LWCONTROL
RWCONTROL
6.25%
12.5%
25%
50%
100%
3.3 x ZID CONCENTRATION: % EFFLUENT
PERCENT SURVIVAL BY REPLICATE
1 2
•>
1
3 4
MEANPERCENT SURVIVAL
LW SURVIVAL >90%?
RW SURVIVAL>90%?
LC50:
LW SURVIVAL >90%?
RW SURVIVAL> 90%?
LC50:
LW SURVIVAL >90%?
RW SURVIVAL> 90%?
LC5(I:
D YESD NO
D YESD NO
D YESD NO
D YESD NO
D YESD NO
D YESD NO
COMMENTS:
2. a:\9731RPT.DOC
CHRONIC TOXICITYTEST RESULTS
CONTROLS RECEIVING WATER LABORATORY WATER
FATHEAD MINNOW CERIODAPHNIA DUBIA FATHEAD MINNOW CERIODAPHNIA DUBIA
SURV. >80%? YES NO X YES NO X YES NO X YES NO
MINIMUMPERFORMANCE
>0 25 MG/LARVA?
X YES NO
>15 NEONATES/SURVIVING FEMALE?
X YES ____ NO
>0.25 MG/LARVA?
X YES
>15 NEONATES/SURVIVING FEMALE?
X YES ____ NO
COMMENTS:
LARVAL FATHEAD MINNOW SURVIVAL & GROWTH TEST
EFFLUENTTREATMENT
PROPORTION OF SURVIVAL BYREPLICATE
MEAN PERCENTSURVIVAL
MEAN LARVAL DRY WEIGHT PERREPLICATE
MEAN BIOMASS(MG)
ICJ5
LW CONTROL 10/10 9/10 10/10 10/10 97.5 0.45 0.41 0.39 0.41 0.42 Inconclusive
RW CONTROL 1/10 7/10 10/10 9/10 67.5 0.06 0..24 0.38 0.38 0.27
6.25' 7/10 4/10 6/10 6/10 57.5 0.37 0.19 0.26 0.27 0.27
12.5% 5/10 6/10 4/10 7/10 55 0.28 0.30 0.15 0.37 0.27
25% 6/10 6/10 4/10 10/10 65 0.35 0.33 0.21 0.38 0.32
50% 10/10 7/10 6/10 6/10 72.5 0.39 0.32 0.32 0.34 0.34
100% 10/10 10/10 6/10 10/10 90 0.50 0.44 0.39 0.45 0.45
COMMENTS: " Primary control did not meet minimum survival criterion; IC25 vs secondary control > 100%.
CERIODAPHNIA DUBIA SURVIVAL & REPRODUCTION TEST
EFFLUENTTREATMENT
NEONATE PRODUCTION BY REPLICATE
10
TOTALNEONATESPRODUCED
%SURVIVING
MEANNEONATESPER INITIAL
FEMALE
IC2S
LW CONTROL 41 41 36 42 43 40 40 42 44 41 410 100 41.0 > 100%
RW CONTROL 45 44 42 40 42 38 37 38 40 37 403 100 40.3
6.25 % 45 43 48 38 44 15 42 39 43 42 399 90 39.9
12.5% 47 45 38 38 41 36 36 39 42 44 406 100 40.6
25% 41 42 40 45 38 38 39 40 36 39 398 100 39.8
50% 39 41 40 40 44 42 42 36 43 41 408 100 40.8
100% 37 29 37 36 35 32 37 34 26 31 334 100 33.4
COMMENTS:
3. a.V9731RPTDOC
SAMPLE INFORMATION :
Sample 1
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
SAMPLETYPE
EFF-24C
EFF-24C
EFF-24C
RIVER-G
SAMPLE COLLECTION
Begin Dale
10/8/97
10/9/97
10/12/97
10/9/97
Begin Time
0630
0700
0630
0730
End Date
10/9/97
10/10/97
10/13/97
NA
End Time
0630
0700
0630
NA
EFFLUENT & RWTEMPERATURE (°C)
Collection
9.0
8.0
9.0
NA
At Lab
3.5
0.4
2.0
7.2
PHat
Lab
8.2
8.5
8.1
7.9
Handdeliver?(If yes, #
hrs.)
Y, S3hr
Y, s2hr
Y, <3hr
Y.i2hr
HoldingTime
<36-h?
YES
YES
YES
YES
YES NO
YES NO
SampleAcceptable?
YES
YES
YES
YES
YES NO
YES NO
DATA SHEET COMPLETED BY:
LABORATORY CONTACT (PLEASE PRINT):
David F. Sanders - Laboratory Director, RMT Applied Biology
PHONE: (920) 830-0209 LAB CERT. NO.: 445142500
PERMITTEE CONTACT (PLEASE PRINT): ,.
Peter J. Glassen, RMT, Inc.
PHONE: (920) 830-0209
SIGNATURE:
DATE: 21 October, 1997
SIGNATURE:
DATE: 21 October, 1997
Send a copy of this form to the following address, according to the timelines specified in your WPDES permit: BiomonitoringCoordinator, Bureau of Watershed Management, Department of Natural Resources, 101 South Webster St., P.O. Box 7921,Madison, Wl 53707-7921
TO BE COMPLETED BY THE DEPARTMENT:
DATE REPORT RECEIVED: RECEIVED WITHIN 45 DAYS?D YES D NO D NA
DID TESTS PASS? ACUTE: C. dubia DYES D NO D Inconclusive/Unacceptable
fathead minnow D YES D NO D Inconclusive/Unacceptable
CHRONIC: C. dubia D YES D NO D Inconclusive/Unacceptable
fathead minnow D YES
RETEST REQUIRED?
RETESTS: ___ ACUTE/CHRONIC
D NO
RESULTS ENTERED INTO DATABASE?
REVIEWED BY:
D Inconclusive/Unacceptable
D YES D NO D NA
DUE TO: D FAILURE D QA/QC PROBLEM
D YES D NO
DATE:
COMMENTS:
CC: AREA ENGINEER/SPECIALIST
PERMIT COORDINATOR
PERMIT FILE
4. a:\9731RPTDOC
Client:
Sampled by:
CHAIN-OF-CUSTODY RECORD
(signature)
INC.
Return Samples to: RMT Applied Biology5191 BAbitz RoadAppleton.Wl54915
SampleIdentification
0 £(
& M
®*ifi-
O
©
#
Containers
/
£
/
Grab
Date/Time
—— .
O"73O
o?y£7
Composite
StartDate/Time
"Zl'£
—
Remarks:
Custody Se
EndDate/Time
o63o—
- —
Ai*>A-€:al: OK/ BiuK.au
Collection
Temp.
i fi
toft
fJft
pH
—
—
——
NHj-N
——
—
——
CI2
——
—
——
Receipt
Ice ^
*5
Vfe3
^S
Laboratory ID: T?3l
0 El CH it3 "
Temp.
3.5-
7. a
2.1
pH-
^
7-1«.
DO-
ItJ U
i*-i(U
C\i'
,0.0!A ) >r^ *j
4.^1
NH3-N-
^V
£[.«<
a]
® eifr. crt 14^ CA.^-o
0 (IV CH l-~7 " ©
Relinquished by Received by:(to carrier) Date/Time Date/Time
i
Relinquished by: ^^^/C-^?^ tf'f-f") ^O^Received for lab: Jgk+A \ {^J^^ J^'t'll /offtDate/Time I/ / Date/Time '
Carrier Identification (UgSr-Eed Ex, Airborne, ate.) &fOflf<5
I:\VVPFV01LAB\CKA1N OOC \l\Tfil
Client:
Sampled by:
CHAIN-OF-CUSTODY RECORD
(signatura)
Return Samples to:
INC.
RMT Applied Biology5191BAbitzRoadAppleton.Wl54915
SampleIdentification
o rr^9 t^(r0
O
©
#
Containers
/
1
Grab
Date/Time
———
ie>-io-«n07/0
CompositeStart
Date/Time
/0"*V99 0~?£6
-—-
EndDate/Time
/0-/0^-)0>v<
—— -
Remarks:
Custody Seal: OK /Bremen
Collection
Temp.CO
$°C
ft°r
pH
——
—
NHj-N
—
-
CI2-t"
"
-
ReceiptIce -
Ye;
Vfe3
Laboratory ID: <)T3l x
o cU 3fV
Temp. "^CO
o.Mo.Y
pH-
^
11
DO-
1
13.V
c.2.
c^-l
6a^l
NH3-N "
^Y -
t^
0o
Q £-^J 3S </ fden^^ " <f)1 '
Relinquished by Received by:(to carrier) Date/Time Date/Time
Relinquished by: ' Z-~*&>1 fc-jcd^L tQ- ld-?l Ofo Received for lab: Tu& l ( jL->4 /o-JO -<H/oS'05~Date/Time VJ ~ t Date/Time /
Carrier Identification (UPS, Fed-Ex, Airborne, etc.) liT*T
HAiriooc \nrnr
Client: Ley»% li>crAj
Sampled by:(signature)
CHAIN-OF-CUSTODY RECORDIHC.
Return Samples to: RMT Applied Biology5191BAbitzRoadAppleton.Wl54915
SampleIdentification
° £ *a\ .
b* j (J&T«J* /
0
O
©
Containers
I
I
.
Grab
Date/Time
/0-/>7?o/fc
0 /7
CompositeStart
Date/Time
AH9-77 ^0
EndDate/Time
{0-(3-fl otjo
Remarks: (jffc^, AfcV*/t'»vt< WI'-HC^ »^ w<yw. l.'w*
NtOA/tCustody Seal: OK /Orphan
Collection
Temp.
O ^/*
1 2 ^/*
PH NHj-N CI2
ReceiptIce-
^
^
Laboratory 10: 1? 1 "
O Ck 5-T
Temp. 'CO
^•0
2.0
pH ^
8.1
*.3
DO^-
»/ .«
n.fc
Cl,<
<0.0 |
..0,^
NHrN -
<,.H '
^
0O
0 CK At:^ 5-1 ©
Relinquished by ^*Vt^ £~*t/ri tff^fj-'fl ^p5?/- Received by:(to carrier) Date/Time Date/Time
Relinquished by: Received for lab: ^^ Ptx)x*3/W. lo-ll-QT / df L>Date/Time Date/Time
Carrier Identification (UPS. Fed-Ex, Airborne, etc.)
rWVPFVOM-AQVCHAIN OOC
Project No:
Client:
Organism:
Seven Day Bioassay Chemical Data - Initial
Start Date: I 0 I / 1 "7 "
Ceriodaphnia dubia -Pimephales promelas
Composite #1 Date:Composite #2 Date:Composite #3 Date:
/o /S-/7?7
TestSolution
QC+ 10%(Y/N)
SecondaryControl
(C)ra&Su)-
Param.D.O.PH
Cond.D.O.PH
Cond.Alk.
Hard.SE/B12 (initials)
PrimaryControl
(1)6<*fJcV R,-
AerationD.O.PH
Cond.Alk.
Hard.Effluent Composite #
c.asvEffluent
(2)ia.r*A>
Effluent(3)
2r*x>'Effluent
(4)50 V
Effluent(5)
]U3 °/0 •
Effluent(6)
AerationD.O.pH
Cond.
D.O.pH
Cond.D.O.pH
Cond.
D.O.PH
Cond.
D.O.PH
Cond.Alk.
Hard.Determined By:
Exposure Date1
YVV2U13oL<nsLt°mete*n•7-TK\3zk310I
^s|
V-*S7-1•7/ry,3"7-1->MT-3*•!lo^
*3ff.*34??<r-3S-T1
^1>tf3^0We-
2
Yyf?,o.i'ipit
r^cv^i-*?•«>^^7<R5~
3
YYY
?.£>
1-^2^^
'ipYE$|
T-«? - Z711
4
YYY
i.°i!.<=)a^i
jfYfs l7-°\
^.2T 12.
5
YYY
"M8.0zq^
i<»Ye*iT.88.3in
6
y/V>1"7-1^Q
^Ycjoa-^•c
?>M
7
YY
Y8 - 1T - T$02
3P
ves i8-2.2.3
T2(p
8
1telT.o^,7>•)l^T.oIK
7(2&ot-a7tftfro?3C^1T-"0
tr-s6^-
r^6-
ZY£S 4l.«^•Zn 12.n.«8.2.lo^•7.°!$.zlooT.I8.3fc838.1o-H(.55M370tf
ZY c $ ln.qS - 2lotT - ^fc-2.T o 3T.I8.2(ff«i5i.q&.Z(.SiS-o«-3kSJ
^r
iYES I
T.S$.iTol
1-S?? • *TozT.XS.l(p'li"M«.l(*15«.o%-3(pH2-2^310X
3V€^<'
^,0
?-M101*.o«•"!C^V^g,o«-H
6^1^.D
«-MCT%S.I«-L/
^5^
5Yesi^.i8.31H«.z^.312 /8.2.8.3T » 2«.2.8.3t.<n^.^S-3l»us
ftl<> iC
Comments
s
^
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^
DO ; a. i - 1
-
^
s
s
s
1\WPFVO\LAB\7DAY1NI.DOC 01/16/97
Seven Day Bioassay Chemical Data - Final
Project No:
Client:
Organism:
TL3 Start Date:
Ceriodaphnia dubia -
TestSolution
QC+ 10%(Y/N)
Secondary<Yi \\SVO -Control
(C)Primary
Oc?(^cK fl-Control
(DOs %'Effluent
(2)
K.$ %'Effluent
(3)
as- %'Effluent
(4)
S6 %'Effluent
(5)
/CO %'Effluent
(6)
Param.D.O.PH
Cond.
D.O.PH
Cond.
D.O.pH
Cond.
D.O.PH
Cond.
D.O.pH
Cond.
D.O.pH
Cond.
D.O.PH
Cond.
D.O.PH
Cond.
Determined By:
Exposure Date1
YV
?,0T1
ff.o?. 4,
^iD
?.6
5.ar.c,
?Q§.4
3,2?'5"
<?>^f.5-
/^
2
y
Y
T - * )a.i ^S.os.i
$.0
S.7
«-oB.(^
S-oS.i«
l-T«.s
ft^»•3-S
i-f
3
YY
l.-L*.\
%-l«.fc
8-1%-(P
^.zS.fe
%-T-?.s
*.l*.H
?.2«-S
>f
4
YV7^T/O
?.o?'"?
^iO
7-7
T,o^•7
^•^y-t
tf.o?-4
1?"y-ir*ra6-
5
YY
T-««.o
1.%M
T - S^.T
T-S8-1
~M8-Vo
l.'fX.t
a.o8-H
K
6
YT
T - T2».o
1.?«.l»
l.««.(.
1-S«.t
1-^$<b
1.°[8.S
1-^^•H
>r
7 8 Comments
s
'
-
-
-
'
-
"
tft.
t\WPFVOM>B\7DAYCER.DOC 01/18/97
Seven Day Bioassay Chemical Data - Final
Project No:
Client:
Organism:
^73 Start Date: 10
Pimephales promelas
TestSolution
QC+ 10%(Y/N)
SecondaryControl
rnHSiO(C)
Primary6i«(Jcy\ n -
Control(1)
£.3^%-Effluent
(2)
Q.S"°&'Effluent
(3)
2s %'Effluent
(4)SO % 'Effluent
(5)
/tf> °/0 "Effluent
(6)
Param.
D.O.pH
Cond.
D.O.PH
Cond.D.O.
PHCond.
D.O.pH
Cond.
D.O.PH
Cond.
D.O.pH
Cond.D.O.PH
Cond.D.O.pH
Cond.Determined By:
Exposure Date1y
V
7/o1.5-
>fey*rC§r**•
(T-^fi^c.-y^•5"
^
1*
t*t-3
KTC
2
YY
U.H1.H
(^•3^1.5
(.-Ms.s
(,.MS.5
(.-M?.S
5.c,
M
ff.i8.2.
>p
3
YY
(o.S
,1-3
(».f3.5
(,.H^•5
t-H^.5
Cr -3
S.H
5-^8,3
5-?2.2.
Jp
4
YY
u.$l.H
lo.i.•8-5
(P. 2.
s.s
t.l§.6
U.|^5.5"
(,.0
«.M
Co-o*,1
^P
5
iY
<a•7-^C£s.r/C-5"?,t
CJ*L
C^S-5-
^3f-r
^V>3
(tf£
6
YY
U.o
1.S
(^.l
«-^
(,.0
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(o-O
S.M
5.98.4
5.Sft.-f
5.?S--2-
>P
7
VVs-v*Y5.4y,f
ji|-ii-^(,f-Y
15-y"/^«-Y
1'?.9
(fTC
8 Comments
*r
-
-
t^-
-
-
u.
-
/>
I:\WPFVO\LAB\7DAYPIM.DOC 01/18/97
Ceriodaphnia dubia Survival & Reproduction DataProject No:
Start Date:
Start Time:
Set by:Age/Source:
Feeding By:
tn3\ -10 /<! If-? x
IbSto -ATC /" <3o<tfJ -*•
^Kr -A/ 1-3^-17• j *JO fr~u—^ JOd1 (£v_ d2 (CJ*— "3
Client:Finish Date:
Finish Time:
% 3rd Brood:Test Endpoint:
>f d4 X
Le.«Y\b£.rqe.r "10/15^1 ^ -I05o " "Day 6 /oo0/^3^7
(Day ff} ^ • Day 7
d5 flTL, d6 )r d7
Day 8Day 8
d8
Test
Solution
Secondary
Control
(C)
jv\HSU3 ^
Rep.
1
2
3
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day
1
el<
<
a "6
2
6/e
>f
3
S1t>7s7s ••tbZ\t
4
|3^\t|e^1^g£
££^
5
I
13£
/S(H615il\k>13*f
6
20
IIIt.
zoZl
^1202122.2.0
JP
7 8
Comments:
Test
Solution
&c<*tocV\ ^
Primary
Control
d)
Rep.
12
3
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
okt
1
(t£
2
fr/£'
>?
3
sT1-]T-j(9
(o<0
>P
4
|L|
^|3IS£g$g£0(&<s
5
E(5"E£IM
1110
It'i
11>f
6
23a12.2.)
Z l20iO2 2110
*P
7 8
Comments:
Endpoint: Day^/B
Total
Neonates
Ml -"Ml --3k --H3 --S3 -10 --^o -^H^ --H^i --Hi -'
Total Neonates:
Total
Broods
3 .
3 ,
3 -J ,3 '3 •3 ,3 x3 ,3 -
%3fd Brood:
)oo -^
Mortality
K Survival:
|cjO .
Initial Adults: JO ' -•Neonates/lnitial Adult: Hl-0 * -^Surviving Adults: JO '_ -Neonales/Surviving Adult: Hl-° " ^
Endpoint: Da>C0/7/8
Total
Neonates
4S~ '.V1/ v
* ot "^L( /^^\ —
*U '-3Sr -3-7 -.3^ -^fo v37 v
Total Neonates:
Total
Broods
3 -3 -3 >3 .3 ,3 -3 .3 -3 '3 -
%3rd Brood:
(00 -'
Mortality
K Survival:
(DO' **
Initial Adults: |0 ' -Neonates/lnitial Adult: 4^-3Surviving Adults: (0 - •" ^ ____Neonates/Surviving Adult: nOi^ --
G = Gravid G/E = Gravid/Eyed E=Eyed NA- No Animal AD = Adult DeadYD = Young Dead ABY = Aborted Young M= Male K = Killed in Transfer Date Check: >P
PS,
I:\WPFVOVLAB\CERIO.DOC
Ceriodaphnia dubia Survival & Reproduction Data
N o : " C l i e n t :
Day 7
Start Date: \0 1 ^ |
Test Endpoint: Day 6 Day 8
TestSolution
c,ar%'Effluent
(2)
Rep.
123
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
olxi
.
<ftc
2
fr/t
^r
3
$
1fc>
57
••*>b't>t>i>p
4
iH^I*»a^pf£*^/i
5
£/Z££14
9/*DliIIIM13>f
6
i32M
ZH2.123
232 1^322.
df
7 8
Comments:
Endpoint: Day(^7/8Total
Neonates
fr -
V^> --vr -38T -4V —/sr -"va -3^ v-^^ -'H^ -
Total
Broods
33 -3 ,3 -3 -a -? -3 -3 -3 -
Mortality
•S it*
TOTAL % SURVIVAL:NEONATES: ^-y\\/ ^o-INfTIAL ADULTS: NEONATES/ADULT:
ID' 3<t-V-
TestSolution
\3 .S%
Effluent
(3)
Rep.
12
3
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
<k\
.->
to
2
fr/E
»f
3
SL>
k><i>
(o5
<o(011
JP
4
l*f
1"?0IIJ5^L^/^^a^
5
B£12.e13E/ IJ3.1115^P
6
-iHll10
2. \IL20
^20Z\2Z
>r
7 8
Comments:
Endpoint: Day@j7/8Total
Neonates
M7 --Vr -s
1>% -~3t •*HI -~34 ".u --31 •-^ -.^ -
TotalBroods
7-3 .3 -3 ,3 -3 -3 -3 ~3 -3.
Mortality
TOTAL % SURVIVAL:NEONATES: ^
Holo"^ '°°'INmAL ADULTS: NEONATES/ADULT:
fC? - t(6,t '
G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male
AD = Adult DeadK = Killed in Transfer Date Check:
I:\WPFVCALAB\CERIO2.DOC
Ceriodaphnia dubia Survival & Reproduction Data
Project No: ^"73 | - ______ Client: q
Start Date: )C / ^ j *?*7 •"
Test Endpoint: Day 6 ^/' Day 7 Day8
TestSolution
as-%'Effluent
(4)
Rep.1
2
3
45
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
r>k
,
«?•
2
It**
>9
3
&
b\o3kk> .7fc>feV
>P
4
13£
12IS^11^(310|3flr^
5
£|(PEeKE12.£££^f
6
,2120
22.
^321
2120
i\ib10
>P
7 8
Comments:
Endpoint: Da/^7/8
TotalNeonates
HI -Ha --«/0 '-vr --JS^ -,3S -3<1 --Ho ^34 -3^ „
Total
Broods
? .3 -3 -^ -3 -3 -3 '3 -> -3 -
Mortality
TOTAL % SURVIVALNEONATES:
3?8-^ /CO:INmAL ADULTS: NEONATES/ADULT-
/O'- J?.?'
TestSolution
So %'Effluent
(5)
Rep.1
23
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
rite
.IW-
2
6/6
>p
3
5(pkb111t>•7-7it
A
(3^iHrt£iH£I]gftfac^
5
6
Wt
£14£13E«*e»r
6
^1
-il20
Z J23il22
'^^322.
*f
7 8
Comments:
Endpoint: Day®7/B
Total
Neonates
31 xLl( -x
Mt> -VQ-^ -HJ -^^ -.Jt-M3 ^M( ^
Total
Broods
3 -3 -3 -3 -3 -33 -3 '3 -3 -
Mortality
TOTAL % SURVIVAL:NEONATES: / /oCX _- X40* ^- )0oINmAL ADULTS: NEONATES/ADULT:
/o ip.fr ^G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male
AD = Adult DeadK - Killed in Transfer Date Check:
I:\WPFVO\LAB\CERIO2.DOC
project NO:
Ceriodaphnia dubia Survival & Reproduction Data
C|ient:
Start Date: |Q / ^ /*?"? "
Test Endpoint: Day 6 Day? Day 8
Test
Solution
100%"Effluent
(6)
Rep.1
2
3
4
5
6
7
8
9
10
Determined By:
Neonates/Exposure Day1
<S\<
•
(fi*-
2
frfc
1
^P
3
87bbfcIff •1t>55>?
4
Mr?tttt£tE£to
5
£Sli'3(111MII512.><>
6
1$
HIS'1(S»51°)ni<*IH>r
7 8
Comments:
Endpoint: Day@7/8
Total
Neonates
37 vai •-37 o?4 -35^ '-^J3--37 --3V -,34 '.31 x.
TotalBroods
3*-3 .1 -3 -3 -3 -3 -3 ,3 '3 -
Mortality
TOTAL % SURVIVAL:NEONATES: -j 5 (_/
3J( ' /(5t> '-INITIAL ADULTS: NEONATES/ADULT:
/O S 33-M -^
Test
Solution
Effluent
( )
Rep.1
2
3
4
5
6
7
8
g10
Determined By:
Neonates/Exposure Day1 2 3 4 5 6 7 8
Comments:
Endpoint: Day 6/7/8
Total
NeonatesTotal
Broods Mortality
TOTAL * SURVIVAL'NEONATES:
INITIAL ADULTS: NEONATES/ADULT
G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male
AD = Adult DeadK = Killed in Transfer Date Check:
I:\WPFVO\LAB\CERI02.DOC
Pimephales promelas Survival & Growth Data
Project No:
Start Date:
Start Time:l<=l /
Client:Finish Date:
Finish Time:
Set by: «7 C Age: < 24 hour
Test
Solution Rep.
Fatalities / Survivors Per Day
6
Total
Fatal. Surv. Surv.Mean
Surv.
SecondaryControl
(C)
.* o IOOVi
(0D [0
PrimaryControl
d)
0//CJ */!!•
"/l o '/I 0/7 3Via O 10
fill* /c
(o70
%'Effluent
(2)
olio Vii* 70/c| Y
D
70 -57,5
cllo Vs o/S" 5Effluent
(3)
OllO •/*Olio
D olio 0/7 3 7
5*^-s
7C?2 5 %Effluent
(4)
OHO 3/1V(0
olioVlO "/it?
HoIOO
So %Effluent
(5)
Oho o•M* */? 3
D
(oo -70
loo °/0 -Effluent
(6)
Olio •/I. "/To Vtto 0 10Olio 10
oht> Mo V-7 0/7 Moho 10
(oO -
/£*>Determined By:
Comments:
Data Check:
I:\WPFVO\LAB\P1MEPHAL.DOC
Sfu't'
Larval Fathead Minnow Weight
ar ft^VReflg o.«*<i<i<i<i / Kooooa
^ Ref 5g 5*^000 i ' 5i 00003•.«•
/Test
Solutionc.
SecondaryControlMHSW •-
1PrimaryControl
3rantk ft • "
<,•«*<» -Effluent
13)
N.S% -Eflluent
^3)
25*. -Effluent
W /
So*?* -Effluent
C^^)00?0 '
Effluent
(£)Effluent
Rep12341234123412341234123412341234
PanID
ClClt3£MtA/ a1C
101A2.6XC2-0M36ic304A46«4tHO945(b5CSOfc/|fafttc/00
TARE +*^ Sample fe) */•aiwt -y ^/. ^00^1/•:?oato ^ ^/.ayoo^(.30033 '[,m\W1 . 375^ (/.36613L/. s^car/.il1/^/.3?CS"7/. ?65£ 3/.annvH/.as'y^D/. A1830/..lOM?/. 50 /yj/. 3=10%/. 31V5"!f.SS'VO^/.afiT35t/. afcoJ^l/ .a7?(// . 306/31- ~*>0%13.h a.-^ooir/• 97 //a1-30230
!»/<$• /o// t
? TARE (g)1- 2-7M151. ilfrTH1, 2°l^l II- 2.lS<nI.'ZI^TSI. 2<plQM
1. 271 le«>1. 30290
1. 2<USt,i, z<uiq1. 28H2Ti. 5oia<*i. a'JfciSi. i8 SSS
1. 2TfcTl1- 2^751i, ?.<ri<n. 287W
). 2*?l3q1.280331. 27^2.i. 2571^. 2T*<* 2.
( • 3o lTJi .2qnq/». USTo1. 2(»12)1- 2<n$'S
# Fish ^Biomass
/o
n
s
# Fish __Mn wtft>7
A>/o
/7
/O7
I \WPFVCHLAB\FSHWTOAT DOC
Project
Test start
9731
10/15,16/97
Balance Calibration:
TestSolution
SecondaryControl
C
PrimaryControl
1Branch R.
6.25%2
12.50%3
25%4
50%5
100%6
RepABCDABCDABCDABCDABCDABCDABCD
PanIDC1C2C3C41A1B1C1D2A2B2C2D3A3B3C3D4A4B4C4D5A5B5C5D6A6B6C6D
Client
Initials
Yes
Tare +Sample (g)
1.27866 -1.30081 -1.30260 -1.28009 -1.30033 -1.27148 -1.27541 -1.30672-1.29625 -1.29466 -1.28687 -1.30563 -1.29914 -1.28860 -1.29820 *1.30118 <-1.30143 .1.29046 -1.29451 >•1.28408 -1.28351 -1.26039 -1.27811 ^1.30613 -1.30292 .1.30015 -1.27112 x1.30230 ^
Larval Fathead Minnow Weight Data
Lemberger Landfill
TP, RJC
X
Tare (g)1.27413-1.29674 -1.29871 '1.27597-1.29975 -1.26904 -1.27160 -1.30290 -1.29256 .1.29279-1.28427 '1.30289 -1.29635 .1.28558-1.29671 ,1.29751 -1.29797 -1.28714 -1.29239 s1.28033 -1.27962 -1.25716-1.27492 -1.30271 -1.29791 -1.29570 x1.26721 '1. 29783 /-
No
Sample (mg)4.534.073.894.120.582.443.813.823.691.872.6
2.742.793.021.493.673.463.322.123.753.893.233.193.425.014.453.914.47
Biomass# of Fish @ Start
10101010101010101010101010101010101010101010101010101010
Weight (mg)0.450.410.390.410.06 "*0.240.380.380.370.190.260.270.280.300.150.370.350.330.210.38 f0.390.320.320.340.500.4410.390.45
Reference Wgts:
Nominal1.000005.00000
Actual0.99999/1 .000025.00001/5.00003
Mean Wgt.# of Fish @ Term.
109101017109
Weight (mg)0.450.45 -0.390.410.58 .0.35 .0.380.42 "
#DIV/0!#DIV/0!#DIV/OI#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!
MeanBiomass
0.42
0.27
0.27
0.27
0.32
0.34
0.45
MeanWeight
0.43
0.43
#DIV/0!
#DIV/0!
#DIV/0!
#DIV/0!
#DIV/0!
•^ "" /
a:\9731fwc.XLS
Cone. ID
Cone. Tested
ResponseResponseResponseResponseResponseResponseResponseResponseResponseResponse
12345678910
1
0
45-44-42-40-42-38-37-38-40-37,
' 2
6.25
45 -43'48'38'44-15'42'39-43-42-
3
12.5
47-45'38'38'41'36'36'39-42-44-
4
25
41'42-40'45-38'38'39-40.36-39-
5
50
39-41"40'40'44'42-42'36-43-41-
' 6
100
37293736353237342631
*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Landfill "—Test Start Date: 10/9/97 ' Test Ending Date: 10/15/97-_Test Species: Ceriodaphnia dubia 'Test Duration: 6-days 1DATA FILE:
Cone.ID
123456
NumberReplicates
101010101010
Concentration% Effluent
0.6.12.25.50.
100.
000250500000000000
ResponseMeans
40.39.40.39.40.33.
300 *•900"600^800"800-400-
Std. PooledDev. Response Means
293223
.869
.195
.836
.486
.251
.806
40.40.40.40.40.33.
300275275275275400
*** No Linear Interpolation Estimate can be calculated from the *-input data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean. *
,/,./•<
1 ;> I •"';>
Cone. ID
Cone. Tested
ResponseResponseResponseResponse
1234
0
0.06-0.24-0.38-0.38-
2 '
6.25
0.37-0.19-0.26-0.27-
3
12.5
0.28 '0.30'0.15-0.37-
4 ^
25
0.35-0.33'0.21-0.38-
5 ^
50
0.39-0.32-0.32'0.34-
6
100
0.500.440.390.45
*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Effluent "Test Start Date: 10/9/97 ' Test Ending Date: 10/16/97 -Test Species: Pimephales promelas 'Test Duration: 7-days "DATA FILE:
Cone . NumberID Replicates
123456
444444
Concentration% Effluent
0.0006.25012.5'bO25.00050.000100.000
ResponseMeans
0.265-0.273-0.275-0.318'0.343'0.445-
Std. PooledDev. Response Means
0.1520.0740.0920.0750.0330.045
0.3200.3200.3200.3200.3200.320
*** No Linear Interpolation Estimate can be calculated from theinput data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean.
P,
Cone. ID
Cone. Tested
ResponseResponseResponseResponse
1234
1
0
0.45-0.41-0.39-0.41-
2 ^
6.25
0.37-0.19-0.26.0.27.
3 "
12.5
0.28-0.30-0.15-0.37-
4
25
0.35-0.33-0.21'0.38-
5 s
50
0.39-0.32-0.32-0.34'
6
100
0.500.440.390.45
*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Effluent'Test Start Date: 10/9/97 - Test Ending Date: 10/16/97-Test Species: Pimephales promelas'Test Duration: 7-days 'DATA FILE:
Cone.ID
123456
NumberReplicates
444444
Concentration% Effluent
0.0006.25012.50025.00050.000100.000
ResponseMeans
0.4150.2730.2750.3180.3430.445
Std. PooledDev. Response Means
0.0250.0740.0920.0750.0330.045
0.4150.3310.3310.3310.3310.331
*** No Linear Interpolation Estimate can be calculated from theinput data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean.
±L- I ff° rr>ei'e"f "i-A _ /—•———~
Sanders, Dave F
From:To:Subject:Date:
Palafox, Dennis (Sidney)Sanders, Dave FSediment Quality CriteriaMonday, October 20, 1997 6: 17PM
I need some help with determining appropriate SQCs for an ecological risk assessment. As you know,SQCs are calculated according to the following equation:
SQC = foc*Koc*FCV
where: foe = mass fraction of organic carbon for sedimentKoc = organic carbon partition coefficientFCV = final chronic value, from chronic ambient water quality criteria
Sediment values are normalized to 1 % organic carbon. My question is -- can I recalculate a new SQC if myTOC values are greater than 1 %? If so, can I substitute the TOC percentage directly into the equation forfoe or do I have to is-4here some type of manipulation before I can use it. What do you think?
-^ •>Thanks,
Dennis
.01
Page 1