mRNA Localization

•  Export

•  Transport

•  Anchoring

•  Selection

Jansen RP, Nature Reviews, Molecular Cell Biology 2001

Oocyte Determination

A PAR-1–dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the

Drosophila oocyte

Richard M. Parton, Russell S. Hamilton, Graeme Ball, Lei Yang, C. Fiona Cullen, Weiping Lu, Hiroyuki Ohkura, and Ilan Davis

JCB Volume 194(1):121-135

July 11, 2011

© 2011 Parton et al.

A dynamic network of MTs extends throughout the oocyte posterior.

Parton R et al. JCB 2011;194:121-135

© 2011 Parton et al.

EB1 tracks the plus ends of dynamic MTs throughout the oocyte.

Parton R et al. JCB 2011;194:121-135 © 2011 Parton et al.

Staufen protein is transported on MTs at the oocyte posterior.

Parton R et al. JCB 2011;194:121-135 © 2011 Parton et al.

Analysis of EB1 trajectories reveals a graded bias in MT orientation.

Parton R et al. JCB 2011;194:121-135 © 2011 Parton et al.

MT nucleation occurs at discrete foci along the cortex but is absent from the posterior.

Parton R et al. JCB 2011;194:121-135 © 2011 Parton et al.

MTs are nucleated around the entire posterior in par-1 hypomorphic mutant oocytes, abolishing the orientation bias.

Parton R et al. JCB 2011;194:121-135

© 2011 Parton et al.

A biased random organization of MTs in the oocyte delivers cargoes to the posterior.

Parton R et al. JCB 2011;194:121-135

© 2011 Parton et al.

Podcast biosights: July 11, 2011 Oocyte microtubules show their bias Video (~9 min) http://jcb.rupress.org/content/194/1/121/suppl/DC2

Model for bcd mRNA localization in late oogenesis.

Weil T T et al. Development 2010;137:169-176

Morphogen Patterning in

Drosophila melanogaster embryos

Kraus Lab fly-fish.ccbr.utoronto.ca YouTube: “RNA localization patterns in Drosophila Embryos” (10min)

The Small Lab- New York University http://biology.as.nyu.edu/object/StephenSmall.html

Strategies employed to interpret graded signals.

A morphogen is a diffusible substance that specifies the fate and activity of developing cells by its concentration.

Porcher, A and Dostatni N, (2010)

Drocco, JA- PhD Thesis, Princeton University

Drocco, JA- PhD Thesis, Princeton University

Porcher, A and Dostatni N, (2010)

Gilbert, S. (2003). Developmental Biology. Sunderland, Massachuetts, Sinauer Associates, Inc. <http://www.devbio.com>

Carroll, SB et al (2001) From DNA to Diversity: Molecular Genetics and the Evolution of Animal Design

The Genetic regulation of segmentation

Pair-rule gene mutant analysis.

Schroeder M D et al. Development 2011;138:3067-3078

Myers, PZ-”Transcription Factors and Morphogens” www.scienceblogs.com

Ibioseminars: Eric Wieschaus (Princeton Univ.) Part 1-Patterning Development of the Embryo (28 min) Part 2-Stability of Morphogen Gradients (37 min) Part 3-Evolution of Bicoid-based Patterning of the Diptera (21 min)