Monthly Report: Surveillance Project SB4008 IFNg tests for ...data.defra.gov.uk/Agriculture/APHA_Gamma monthly report - October 2018.pdf · SER-NSR 10 0.0 % Total 23976 100.00 % Figure

Monthly Report:Surveillance ProjectSB4008 IFNg tests forbovine tuberculosis

(TC0651 and TC0751)

Number 146

Report Period 1st - 31st October 2018

Issued 13 November 2018

Operational notes on terminology, definitions, re-test and re-samples testoutcomes:

• “Samples” refers to an individual tube of blood taken from an individual animal which issubsequently submitted for testing. It is generally assumed that the number of samples andthe number of animals are analogous as any duplicate sample blood tube from the sameanimal will not be tested. However, minor discrepancies may occur where the same animal istested more than once due to a request for a resample or the animal is tested twice underdifferent categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).

• “Submissions” refers to an individual batch or set of samples received for testing.Submissions may comprise 1 or more samples (for example, if an entire herd is being tested itis usually sent as 1 submission). It is generally assumed that the number of submissions andthe number of herds are analogous.

• Retests are samples where the first ELISA assay fails and the same sample is retested on anew ELISA plate. Each sample can be retested only once. A retest is not a reportable testoutcome.

• Resamples are where a sample has been retested and failed a second time, so that the labrequests a new sample. This is a reportable test outcome.

• Rejects are samples that are not tested by the lab for one of the following reasons: bloodcollected into wrong type of vacutainer, samples that have not been maintained at theappropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, bloodthat is extensively clotted (small clots are OK), samples received after 4pm on the day aftersample collection. Such samples are reported separately in the tables and in figure 5.

• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure ofthe quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) mayindicate compromised blood quality as a result of collection/transportation conditions or duethe animal having an unusually low/suppressed cellular immune response.

• NEG (no-antigen control) is a sample negative control which provides a measure of thebackground antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)may indicate a laboratory procedures problem (normally resolved during re-test) or that ananimal has unusually high background levels of IFN-g.

Submission Reason Explanation Mandatory?

Possible Herd Slaughter(PAR-HERD-S)

Parallel interferon-gamma blood testing of skintest negative cattle to inform whole or partialherd slaughters decisions

YES, if APHA arecontemplating a herdslaughter

Persistent TB breakdowns(OTF status withdrawn)(PAR-P-CONF) N.B.PAR-PERSIS from 1st April2017

Parallel blood testing of skin test-negative cattlein persistently infected herds that have failed toresolve by repeated short-interval skin testingand fulfill a minimum of biosecurity standards.

NO (APHA discretion)

Parallel - Low Incidence(PAR-LOW-IN) N.B.PAR-NEW-IN from 1st April2017

Parallel blood testing to maximise theprobability of removing all infected cattle in anew herd breakdown as soon as possible afterconfirmation of TB.

YES in Area of LowIncidence

Rapid Testing of twice IR's(PAR-RAPID)

Parallel blood testing of two-times IRs identifiedunder the severe interpretation of the skin testused in Wales.

YES (Wales only)

PAR-CUL-N

Parallel blood testing to maximise theprobability of removing all infected cattle in anew breakdown as soon as possible afterconfirmation of TB where herd is situated inbadger control areas of England that havecompleted at least 2 effective culls.

YES (in eligible badgercontrol areas of England)

PAR-CUL-P

Parallel blood testing to maximise theprobability of removing all infected cattle in apersistent confirmed breakdown where herd issituated within badger control areas of Englandthat have completed at least 2 effective culls


Parallel Other(PAR-OTHER)

Other parallel blood testing not covered in anyof the other scenarios NO (APHA discretion)

'NSR' Herds (SER-NSR)

Modified serial blood testing of individual skintest reactors and/or IRs in unconfirmed TBbreakdown herds to clarify their infection statuswhere there is evidence of non-specificsensitisation to bovine tuberculin (the“non-specific reactor” procedure)


Suspected Fraud(SER-FRAUD)

Modified serial blood test of suspectedfraudulent reactors to the skin test (animals withabnormal skin swellings), in confirmed orunconfirmed TB incidents.

NO (APHA discretion tofirm up or rule out anysuspicion of fraud andsupport anyinvestigations)

Serial Other(SER-OTHER) Serial test – other reasons NO (APHA Discretion)

SER-FLEXI

Extended blood test to provide flexible testreadout in a confirmed M. bovis-infected herdwhere Johne's (M. a. paratuberculosis) infectionor vaccination is suspected to be interferringwith M. bovis infection detection


Test Criteria

NumsamplesOctober2018

% Total 2018 %

Submissions 274 2159

Samples Total (%) 23976 100.00 % 207362 100.00 %

England 19262 80.34 % 160008 77.16 %

Wales 4699 19.60 % 45113 21.76 %

Scotland 15 0.06 % 2241 1.08 %

Parallel Tests PAR-CUL-N 4511 18.81 % 24638 11.88 %

PAR-CUL-P 596 2.49 % 6155 2.97 %

PAR-HERD-S 423 1.76 % 5696 2.75 %

PAR-NEW-IN 11297 47.12 % 109509 52.81 %

PAR-OTHER 1235 5.15 % 2585 1.25 %

PAR-PERSIS 4932 20.57 % 49364 23.81 %

PAR-RAPID 163 0.68 % 1315 0.63 %

Total(% of all samples) 23157 96.58 % 199262 96.09 %

Serial/Extended Tests SER-FLEXI 809 3.37 % 7801 3.76 %

SER-FRAUD 0 0.00 % 270 0.13 %

SER-NSR 10 0.04 % 29 0.01 %

SER-OTHER 0 0.00 %

Total(% of all samples) 819 3.42 % 8100 3.91 %

Total 23976 100.00 % 207362 100.00 %

Retests Total (% of all samples) 1860 7.76 % 16682 8.04 %

England 1562 6.51 % 12756 6.15 %

Wales 297 1.24 % 3725 1.80 %

Scotland 1 0.00 % 201 0.10 %

Resamples Total (% of all samples) 1145 4.78 % 11573 5.58 %

England 974 4.06 % 8723 4.21 %

Wales 170 0.71 % 2711 1.31 %

Scotland 1 0.00 % 139 0.07 %

Rejects Total (% of all samples) 56 0.23 % 1923 0.93 %

England 11 0.05 % 787 0.38 %

Wales 45 0.19 % 1136 0.55 %

Scotland 0 0.00 %

Table 1. Monthly Totals for October 2018

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

PAR-CUL-N

PAR-CUL-P

PAR-HERD-S

PAR-NEW-IN

PAR-OTHER

PAR-PERSIS

PAR-RAPID

SER-FLEXI

SER-NSR

Breakdown ofReason forsubmission

PAR-CUL-N 4511 18.8 %

PAR-CUL-P 596 2.5 %

PAR-HERD-S 423 1.8 %

PAR-NEW-IN 11297 47.1 %

PAR-OTHER 1235 5.2 %

PAR-PERSIS 4932 20.6 %

PAR-RAPID 163 0.7 %

SER-FLEXI 809 3.4 %

SER-NSR 10 0.0 %

Total 23976 100.00 %

Figure 1. Number of samples submitted in each category during October 2018

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

0

500

1000

1500

2000

2500

3000

3500

4000

Aberdeenshire

W Glamorgan

Shropshire

Powys

Pembs

NE Wales

Gwent

Ceredigion

Carms

W Sussex

Wiltshire

Warks

Somerset

Shropshire

Oxon

Notts

Northants

Leics & Rut

Kent

Heref

Hampshire

Gloucs

E Sussex

Dorset

Devon

Derbyshire

Cumbria

Corn & Scilly

Cheshire

Bucks

Berks

Avon

Scotland

Wales

England

SER-NSR

SER-FLEXI

PAR-RAPID

PAR-PERSIS

PAR-OTHER

PAR-NEW-IN

PAR-HERD-S

PAR-CUL-P

PAR-CUL-N

Breakdown ofSamples

submitted byCounty

Figure 2. Number of samples submitted in each category by County during October 2018

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallelother, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serialpotential fraud, SER-OTHER: serial other

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WigtownWest lothian

PerthshireAyrshire

Argyll & ButeAberdeenshireW Glamorgan

S WalesShropshire

PowysPembs

PembrokshireNE Wales

Mid glamorganGwynedd

GwentCeredigion

CarmsW Sussex

W MidlandsWiltshire

WarksS Yorks

StaffsSomerset

ShropshireOxon

N YorksNotts

NorthantsNorfolk

MerseysideLincs

Leics & RutKent

Isle of wightHumberside

HertsHeref

HampshireGloucs

E SussexEssex

DorsetDevon

DerbyshireCumbria

Corn & ScillyCheshire

BucksBerksBedsAvon

Scotland

Wales

England

SER-NSR SER-FRAUD SER-FLEXI PAR-RAPID PAR-PERSIS PAR-OTHER PAR-NEW-IN PAR-HERD-S

PAR-CUL-P PAR-CUL-N

Breakdownof Samplessubmittedby County

Figure 3. Number of samples submitted in each category by County during 2018

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrentJohne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for October 2018

Country County SubmissionReasons*

No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject

n % of total n % n % n %

England - HRA Avon PAR-PERSIS 1 8 0.03 % 0 0.00 % 4 50.00 % 4 50.00 % 4 50.00 % 0 0.00 %

Corn & Scilly PAR-CUL-N 13 1,166 4.86 % 35 3.00 % 1054 90.39 % 101 8.66 % 77 6.60 % 0 0.00 %

PAR-PERSIS 1 14 0.06 % 0 0.00 % 14 100.00 % 2 14.29 % 0 0.00 % 0 0.00 %

Devon PAR-CUL-N 9 2,042 8.52 % 171 8.37 % 1778 87.07 % 163 7.98 % 90 4.41 % 3 0.15 %

PAR-CUL-P 1 265 1.11 % 6 2.26 % 255 96.23 % 6 2.26 % 4 1.51 % 0 0.00 %

PAR-NEW-IN 2 548 2.29 % 29 5.29 % 505 92.15 % 19 3.47 % 14 2.55 % 0 0.00 %

PAR-PERSIS 3 745 3.11 % 31 4.16 % 692 92.89 % 48 6.44 % 22 2.95 % 0 0.00 %

Dorset PAR-CUL-N 3 319 1.33 % 21 6.58 % 287 89.97 % 11 3.45 % 9 2.82 % 2 0.63 %

PAR-CUL-P 1 271 1.13 % 13 4.80 % 239 88.19 % 26 9.59 % 19 7.01 % 0 0.00 %

PAR-OTHER 2 98 0.41 % 7 7.14 % 80 81.63 % 15 15.31 % 11 11.22 % 0 0.00 %

SER-FLEXI 1 69 0.29 % 4 5.80 % 62 89.86 % 3 4.35 % 3 4.35 % 0 0.00 %

Gloucs PAR-CUL-N 4 512 2.14 % 22 4.30 % 460 89.84 % 35 6.84 % 30 5.86 % 0 0.00 %

PAR-CUL-P 3 60 0.25 % 0 0.00 % 57 95.00 % 9 15.00 % 3 5.00 % 0 0.00 %

PAR-PERSIS 4 617 2.57 % 57 9.24 % 540 87.52 % 56 9.08 % 20 3.24 % 0 0.00 %

SER-FLEXI 2 198 0.83 % 16 8.08 % 179 90.40 % 14 7.07 % 2 1.01 % 1 0.51 %

Heref PAR-CUL-N 3 196 0.82 % 9 4.59 % 173 88.27 % 17 8.67 % 14 7.14 % 0 0.00 %

PAR-PERSIS 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Shropshire PAR-OTHER 5 1,117 4.66 % 64 5.73 % 1015 90.87 % 51 4.57 % 37 3.31 % 1 0.09 %

Somerset PAR-CUL-N 5 276 1.15 % 9 3.26 % 255 92.39 % 13 4.71 % 10 3.62 % 2 0.72 %

Wiltshire PAR-NEW-IN 1 343 1.43 % 33 9.62 % 300 87.46 % 25 7.29 % 10 2.92 % 0 0.00 %

PAR-PERSIS 3 1,036 4.32 % 51 4.92 % 958 92.47 % 36 3.47 % 27 2.61 % 0 0.00 %

SER-FLEXI 2 48 0.20 % 5 10.42 % 40 83.33 % 4 8.33 % 3 6.25 % 0 0.00 %

England - Edge Berks PAR-NEW-IN 3 51 0.21 % 0 0.00 % 50 98.04 % 1 1.96 % 1 1.96 % 0 0.00 %

Bucks PAR-NEW-IN 2 24 0.10 % 5 20.83 % 17 70.83 % 3 12.50 % 2 8.33 % 0 0.00 %

Cheshire PAR-NEW-IN 25 2,744 11.44 % 131 4.77 % 2385 86.92 % 327 11.92 % 228 8.31 % 0 0.00 %

PAR-PERSIS 1 11 0.05 % 0 0.00 % 8 72.73 % 3 27.27 % 3 27.27 % 0 0.00 %

Derbyshire PAR-NEW-IN 22 3,094 12.90 % 123 3.98 % 2799 90.47 % 307 9.92 % 172 5.56 % 0 0.00 %

E Sussex PAR-NEW-IN 1 84 0.35 % 10 11.90 % 69 82.14 % 7 8.33 % 5 5.95 % 0 0.00 %

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)

Table 2a. Summary by County for October 2018 Cont.




Hampshire PAR-NEW-IN 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-NSR 1 10 0.04 % 0 0.00 % 10 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Leics & Rut PAR-NEW-IN 7 292 1.22 % 28 9.59 % 257 88.01 % 17 5.82 % 7 2.40 % 0 0.00 %

Northants PAR-NEW-IN 2 120 0.50 % 8 6.67 % 107 89.17 % 10 8.33 % 5 4.17 % 0 0.00 %

Notts PAR-NEW-IN 2 59 0.25 % 4 6.78 % 49 83.05 % 7 11.86 % 6 10.17 % 0 0.00 %

Oxon PAR-NEW-IN 9 1,519 6.34 % 110 7.24 % 1322 87.03 % 134 8.82 % 87 5.73 % 0 0.00 %

Warks PAR-NEW-IN 4 228 0.95 % 10 4.39 % 211 92.54 % 9 3.95 % 5 2.19 % 2 0.88 %

England - LRA Cumbria PAR-NEW-IN 3 409 1.71 % 9 2.20 % 378 92.42 % 36 8.80 % 22 5.38 % 0 0.00 %

Kent PAR-NEW-IN 3 649 2.71 % 33 5.08 % 599 92.30 % 37 5.70 % 17 2.62 % 0 0.00 %

W Sussex PAR-NEW-IN 1 18 0.08 % 0 0.00 % 13 72.22 % 6 33.33 % 5 27.78 % 0 0.00 %

England 157 19262 80.34 % 1054 5.47 % 17223 89.41 % 1562 8.11 % 974 5.06 % 11 0.06 %

Wales - High TB Carms PAR-HERD-S 3 355 1.48 % 19 5.35 % 328 92.39 % 13 3.66 % 8 2.25 % 0 0.00 %

PAR-PERSIS 8 792 3.30 % 76 9.60 % 677 85.48 % 54 6.82 % 38 4.80 % 1 0.13 %

PAR-RAPID 9 66 0.28 % 9 13.64 % 25 37.88 % 5 7.58 % 3 4.55 % 29 43.94 %

SER-FLEXI 4 119 0.50 % 13 10.92 % 97 81.51 % 6 5.04 % 5 4.20 % 4 3.36 %

Ceredigion PAR-OTHER 1 9 0.04 % 2 22.22 % 7 77.78 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %

PAR-RAPID 3 7 0.03 % 1 14.29 % 6 85.71 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Gwent PAR-HERD-S 1 5 0.02 % 0 0.00 % 5 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 3 209 0.87 % 39 18.66 % 161 77.03 % 43 20.57 % 9 4.31 % 0 0.00 %

PAR-RAPID 2 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Pembs PAR-HERD-S 1 1 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 10 753 3.14 % 49 6.51 % 679 90.17 % 26 3.45 % 19 2.52 % 6 0.80 %

PAR-RAPID 18 68 0.28 % 22 32.35 % 43 63.24 % 4 5.88 % 3 4.41 % 0 0.00 %

SER-FLEXI 10 285 1.19 % 27 9.47 % 245 85.96 % 15 5.26 % 12 4.21 % 1 0.35 %

Powys PAR-HERD-S 1 8 0.03 % 0 0.00 % 8 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-NEW-IN 4 163 0.68 % 11 6.75 % 143 87.73 % 12 7.36 % 7 4.29 % 2 1.23 %




Table 2a. Summary by County for October 2018 Cont.




PAR-PERSIS 5 642 2.68 % 60 9.35 % 557 86.76 % 40 6.23 % 25 3.89 % 0 0.00 %

PAR-RAPID 2 10 0.04 % 1 10.00 % 9 90.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 1 4 0.02 % 0 0.00 % 4 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales - Intermediate TB Ceredigion PAR-HERD-S 1 54 0.23 % 0 0.00 % 51 94.44 % 6 11.11 % 3 5.56 % 0 0.00 %

SER-FLEXI 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %

NE Wales PAR-NEW-IN 10 578 2.41 % 12 2.08 % 550 95.16 % 38 6.57 % 16 2.77 % 0 0.00 %

PAR-PERSIS 1 101 0.42 % 3 2.97 % 91 90.10 % 8 7.92 % 7 6.93 % 0 0.00 %

PAR-RAPID 3 9 0.04 % 2 22.22 % 7 77.78 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 4 82 0.34 % 7 8.54 % 71 86.59 % 5 6.10 % 4 4.88 % 0 0.00 %

Powys PAR-NEW-IN 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %

Shropshire PAR-NEW-IN 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

W Glamorgan PAR-OTHER 1 11 0.05 % 5 45.45 % 6 54.55 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales - Low TB NE Wales PAR-NEW-IN 3 354 1.48 % 4 1.13 % 340 96.05 % 19 5.37 % 8 2.26 % 2 0.56 %

PAR-RAPID 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales 115 4699 19.60 % 363 7.73 % 4121 87.70 % 297 6.32 % 170 3.62 % 45 0.96 %

Scotland - Scotland Aberdeenshire PAR-NEW-IN 2 15 0.06 % 0 0.00 % 14 93.33 % 1 6.67 % 1 6.67 % 0 0.00 %

Scotland 2 15 0.06 % 0 0.00 % 14 93.33 % 1 6.67 % 1 6.67 % 0 0.00 %

Grand Total Sum: 274 23976 1417 21358 1860 1145 56

*For test criteria please refer to Operational notes.




Table 2b. Summary by County for 2018




England - HRA Avon PAR-OTHER 1 4 0.00 % 0 0.00 % 2 50.00 % 2 50.00 % 2 50.00 % 0 0.00 %

PAR-PERSIS 8 995 0.48 % 59 5.93 % 849 85.33 % 104 10.45 % 86 8.64 % 1 0.10 %

SER-FLEXI 2 401 0.19 % 55 13.72 % 309 77.06 % 43 10.72 % 37 9.23 % 0 0.00 %

SER-FRAUD 1 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Corn & Scilly PAR-CUL-N 36 5,507 2.66 % 226 4.10 % 5014 91.05 % 406 7.37 % 267 4.85 % 0 0.00 %

PAR-CUL-P 1 10 0.00 % 0 0.00 % 8 80.00 % 4 40.00 % 2 20.00 % 0 0.00 %

PAR-NEW-IN 1 121 0.06 % 18 14.88 % 98 80.99 % 14 11.57 % 5 4.13 % 0 0.00 %

PAR-OTHER 3 228 0.11 % 6 2.63 % 209 91.67 % 21 9.21 % 13 5.70 % 0 0.00 %

PAR-PERSIS 22 4,178 2.01 % 239 5.72 % 3783 90.55 % 240 5.74 % 156 3.73 % 0 0.00 %

SER-FLEXI 9 1,179 0.57 % 96 8.14 % 1028 87.19 % 54 4.58 % 47 3.99 % 8 0.68 %

Devon PAR-CUL-N 42 6,524 3.15 % 464 7.11 % 5627 86.25 % 524 8.03 % 324 4.97 % 109 1.67 %

PAR-CUL-P 18 5,214 2.51 % 301 5.77 % 4621 88.63 % 287 5.50 % 192 3.68 % 100 1.92 %

PAR-NEW-IN 9 904 0.44 % 67 7.41 % 785 86.84 % 62 6.86 % 40 4.42 % 12 1.33 %

PAR-OTHER 5 36 0.02 % 1 2.78 % 30 83.33 % 5 13.89 % 5 13.89 % 0 0.00 %

PAR-PERSIS 34 6,090 2.94 % 314 5.16 % 5348 87.82 % 537 8.82 % 414 6.80 % 14 0.23 %

SER-FLEXI 14 2,191 1.06 % 160 7.30 % 1967 89.78 % 162 7.39 % 61 2.78 % 3 0.14 %

Dorset PAR-CUL-N 46 6,699 3.23 % 380 5.67 % 5935 88.60 % 498 7.43 % 381 5.69 % 3 0.04 %

PAR-CUL-P 1 271 0.13 % 13 4.80 % 239 88.19 % 26 9.59 % 19 7.01 % 0 0.00 %

PAR-NEW-IN 2 636 0.31 % 8 1.26 % 558 87.74 % 73 11.48 % 70 11.01 % 0 0.00 %

PAR-OTHER 2 98 0.05 % 7 7.14 % 80 81.63 % 15 15.31 % 11 11.22 % 0 0.00 %

PAR-PERSIS 13 5,769 2.78 % 263 4.56 % 5238 90.80 % 392 6.79 % 267 4.63 % 1 0.02 %

SER-FLEXI 1 69 0.03 % 4 5.80 % 62 89.86 % 3 4.35 % 3 4.35 % 0 0.00 %

Gloucs PAR-CUL-N 39 4,097 1.98 % 149 3.64 % 3659 89.31 % 477 11.64 % 286 6.98 % 3 0.07 %

PAR-CUL-P 9 560 0.27 % 21 3.75 % 471 84.11 % 92 16.43 % 68 12.14 % 0 0.00 %

PAR-NEW-IN 2 186 0.09 % 5 2.69 % 160 86.02 % 27 14.52 % 21 11.29 % 0 0.00 %

PAR-OTHER 2 180 0.09 % 30 16.67 % 141 78.33 % 17 9.44 % 9 5.00 % 0 0.00 %

PAR-PERSIS 4 617 0.30 % 57 9.24 % 540 87.52 % 56 9.08 % 20 3.24 % 0 0.00 %

SER-FLEXI 5 1,342 0.65 % 45 3.35 % 1247 92.92 % 76 5.66 % 49 3.65 % 1 0.07 %




Table 2b. Summary by County for 2018 Cont.




Heref PAR-CUL-N 22 749 0.36 % 60 8.01 % 630 84.11 % 78 10.41 % 59 7.88 % 0 0.00 %

PAR-CUL-P 2 78 0.04 % 3 3.85 % 72 92.31 % 4 5.13 % 3 3.85 % 0 0.00 %

PAR-NEW-IN 3 466 0.22 % 25 5.36 % 429 92.06 % 39 8.37 % 12 2.58 % 0 0.00 %

PAR-PERSIS 4 232 0.11 % 14 6.03 % 207 89.22 % 11 4.74 % 10 4.31 % 1 0.43 %

SER-FLEXI 2 246 0.12 % 35 14.23 % 200 81.30 % 27 10.98 % 11 4.47 % 0 0.00 %

SER-FRAUD 2 38 0.02 % 1 2.63 % 36 94.74 % 2 5.26 % 1 2.63 % 0 0.00 %

Shropshire PAR-OTHER 7 1,130 0.54 % 64 5.66 % 1027 90.88 % 52 4.60 % 38 3.36 % 1 0.09 %

PAR-PERSIS 12 1,613 0.78 % 125 7.75 % 1435 88.96 % 83 5.15 % 53 3.29 % 0 0.00 %

Somerset PAR-CUL-N 19 1,062 0.51 % 50 4.71 % 910 85.69 % 57 5.37 % 50 4.71 % 52 4.90 %

PAR-CUL-P 1 22 0.01 % 1 4.55 % 17 77.27 % 6 27.27 % 4 18.18 % 0 0.00 %

PAR-NEW-IN 1 22 0.01 % 1 4.55 % 16 72.73 % 5 22.73 % 5 22.73 % 0 0.00 %

PAR-OTHER 5 593 0.29 % 40 6.75 % 423 71.33 % 24 4.05 % 22 3.71 % 108 18.21 %

PAR-PERSIS 21 4,736 2.28 % 413 8.72 % 4098 86.53 % 299 6.31 % 204 4.31 % 21 0.44 %

Staffs PAR-PERSIS 3 640 0.31 % 17 2.66 % 588 91.88 % 33 5.16 % 18 2.81 % 17 2.66 %

SER-FLEXI 2 400 0.19 % 41 10.25 % 337 84.25 % 22 5.50 % 22 5.50 % 0 0.00 %

Wiltshire PAR-NEW-IN 1 343 0.17 % 33 9.62 % 300 87.46 % 25 7.29 % 10 2.92 % 0 0.00 %

PAR-OTHER 2 46 0.02 % 13 28.26 % 31 67.39 % 2 4.35 % 2 4.35 % 0 0.00 %

PAR-PERSIS 11 1,701 0.82 % 70 4.12 % 1582 93.00 % 72 4.23 % 49 2.88 % 0 0.00 %

SER-FLEXI 2 48 0.02 % 5 10.42 % 40 83.33 % 4 8.33 % 3 6.25 % 0 0.00 %

SER-FRAUD 2 9 0.00 % 0 0.00 % 9 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

W Midlands PAR-PERSIS 1 281 0.14 % 1 0.36 % 265 94.31 % 16 5.69 % 14 4.98 % 1 0.36 %

SER-FRAUD 3 120 0.06 % 2 1.67 % 111 92.50 % 10 8.33 % 7 5.83 % 0 0.00 %

England - Edge Berks PAR-NEW-IN 35 3,087 1.49 % 148 4.79 % 2740 88.76 % 347 11.24 % 199 6.45 % 0 0.00 %

PAR-PERSIS 6 105 0.05 % 6 5.71 % 95 90.48 % 7 6.67 % 4 3.81 % 0 0.00 %

Bucks PAR-NEW-IN 19 2,624 1.27 % 121 4.61 % 2271 86.55 % 143 5.45 % 99 3.77 % 133 5.07 %

Cheshire PAR-NEW-IN 170 32,960 15.89 % 1148 3.48 % 30109 91.35 % 2467 7.48 % 1702 5.16 % 1 0.00 %

PAR-PERSIS 10 2,218 1.07 % 139 6.27 % 1770 79.80 % 235 10.60 % 207 9.33 % 102 4.60 %

Derbyshire PAR-NEW-IN 94 12,293 5.93 % 419 3.41 % 11110 90.38 % 1122 9.13 % 761 6.19 % 3 0.02 %








E Sussex PAR-NEW-IN 24 2,904 1.40 % 127 4.37 % 2615 90.05 % 270 9.30 % 160 5.51 % 2 0.07 %

Hampshire PAR-NEW-IN 30 2,759 1.33 % 103 3.73 % 2508 90.90 % 213 7.72 % 148 5.36 % 0 0.00 %

PAR-OTHER 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %

PAR-PERSIS 1 19 0.01 % 1 5.26 % 16 84.21 % 7 36.84 % 2 10.53 % 0 0.00 %

SER-NSR 3 16 0.01 % 0 0.00 % 16 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Leics & Rut PAR-NEW-IN 40 3,657 1.76 % 167 4.57 % 3242 88.65 % 342 9.35 % 246 6.73 % 2 0.05 %

SER-NSR 1 13 0.01 % 0 0.00 % 13 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Northants PAR-NEW-IN 27 1,534 0.74 % 79 5.15 % 1372 89.44 % 124 8.08 % 83 5.41 % 0 0.00 %

Notts PAR-NEW-IN 19 1,498 0.72 % 51 3.40 % 1336 89.19 % 150 10.01 % 111 7.41 % 0 0.00 %

PAR-OTHER 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Oxon PAR-NEW-IN 85 8,950 4.32 % 541 6.04 % 7832 87.51 % 846 9.45 % 568 6.35 % 9 0.10 %

PAR-PERSIS 8 1,643 0.79 % 63 3.83 % 1508 91.78 % 82 4.99 % 72 4.38 % 0 0.00 %

Warks PAR-NEW-IN 68 5,384 2.60 % 260 4.83 % 4717 87.61 % 496 9.21 % 331 6.15 % 76 1.41 %

PAR-PERSIS 2 23 0.01 % 1 4.35 % 19 82.61 % 3 13.04 % 3 13.04 % 0 0.00 %

England - LRA Beds PAR-NEW-IN 3 561 0.27 % 12 2.14 % 453 80.75 % 124 22.10 % 96 17.11 % 0 0.00 %

Cumbria PAR-NEW-IN 31 3,438 1.66 % 85 2.47 % 3180 92.50 % 218 6.34 % 173 5.03 % 0 0.00 %

PAR-PERSIS 1 330 0.16 % 11 3.33 % 304 92.12 % 18 5.45 % 15 4.55 % 0 0.00 %

Essex PAR-NEW-IN 3 493 0.24 % 31 6.29 % 456 92.49 % 26 5.27 % 3 0.61 % 3 0.61 %

Herts PAR-NEW-IN 4 366 0.18 % 5 1.37 % 325 88.80 % 61 16.67 % 36 9.84 % 0 0.00 %

Humberside PAR-NEW-IN 2 99 0.05 % 8 8.08 % 86 86.87 % 8 8.08 % 5 5.05 % 0 0.00 %

Isle of wight PAR-NEW-IN 2 146 0.07 % 8 5.48 % 115 78.77 % 35 23.97 % 23 15.75 % 0 0.00 %

Kent PAR-NEW-IN 3 649 0.31 % 33 5.08 % 599 92.30 % 37 5.70 % 17 2.62 % 0 0.00 %

Lincs PAR-NEW-IN 8 252 0.12 % 12 4.76 % 216 85.71 % 32 12.70 % 24 9.52 % 0 0.00 %

Merseyside PAR-NEW-IN 2 34 0.02 % 0 0.00 % 20 58.82 % 15 44.12 % 14 41.18 % 0 0.00 %

Norfolk PAR-NEW-IN 4 455 0.22 % 9 1.98 % 409 89.89 % 48 10.55 % 37 8.13 % 0 0.00 %

N Yorks PAR-NEW-IN 6 942 0.45 % 30 3.18 % 866 91.93 % 79 8.39 % 46 4.88 % 0 0.00 %

S Yorks PAR-NEW-IN 2 131 0.06 % 11 8.40 % 110 83.97 % 10 7.63 % 10 7.63 % 0 0.00 %

W Sussex PAR-NEW-IN 11 1,442 0.70 % 81 5.62 % 1300 90.15 % 87 6.03 % 61 4.23 % 0 0.00 %








PAR-PERSIS 2 297 0.14 % 6 2.02 % 277 93.27 % 15 5.05 % 14 4.71 % 0 0.00 %

England 1188 160008 77.16 % 7718 4.82 % ###### 89.23 % 12756 7.97 % 8723 5.45 % 787 0.49 %

Wales - High TB Carms PAR-HERD-S 29 1,173 0.57 % 76 6.48 % 1042 88.83 % 79 6.73 % 53 4.52 % 2 0.17 %

PAR-OTHER 6 53 0.03 % 6 11.32 % 46 86.79 % 2 3.77 % 1 1.89 % 0 0.00 %

PAR-PERSIS 42 3,862 1.86 % 315 8.16 % 3335 86.35 % 279 7.22 % 206 5.33 % 6 0.16 %

PAR-RAPID 85 396 0.19 % 90 22.73 % 258 65.15 % 27 6.82 % 19 4.80 % 29 7.32 %

SER-FLEXI 10 342 0.16 % 35 10.23 % 255 74.56 % 56 16.37 % 48 14.04 % 4 1.17 %

Ceredigion PAR-HERD-S 2 6 0.00 % 0 0.00 % 4 66.67 % 2 33.33 % 2 33.33 % 0 0.00 %

PAR-OTHER 1 9 0.00 % 2 22.22 % 7 77.78 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 16 1,300 0.63 % 91 7.00 % 1147 88.23 % 70 5.38 % 58 4.46 % 4 0.31 %

PAR-RAPID 28 94 0.05 % 21 22.34 % 73 77.66 % 3 3.19 % 0 0.00 % 0 0.00 %

SER-FLEXI 8 249 0.12 % 24 9.64 % 200 80.32 % 29 11.65 % 22 8.84 % 3 1.20 %

Gwent PAR-HERD-S 5 429 0.21 % 16 3.73 % 395 92.07 % 36 8.39 % 16 3.73 % 2 0.47 %

PAR-NEW-IN 2 17 0.01 % 1 5.88 % 13 76.47 % 4 23.53 % 3 17.65 % 0 0.00 %

PAR-OTHER 2 3 0.00 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 37 2,270 1.09 % 118 5.20 % 1979 87.18 % 304 13.39 % 170 7.49 % 3 0.13 %

PAR-RAPID 32 170 0.08 % 28 16.47 % 128 75.29 % 14 8.24 % 14 8.24 % 0 0.00 %

SER-FLEXI 6 205 0.10 % 3 1.46 % 179 87.32 % 37 18.05 % 23 11.22 % 0 0.00 %

Pembrokshire PAR-OTHER 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %

Pembs PAR-HERD-S 29 2,521 1.22 % 53 2.10 % 1989 78.90 % 160 6.35 % 115 4.56 % 364 14.44 %

PAR-OTHER 4 34 0.02 % 7 20.59 % 27 79.41 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 77 6,439 3.11 % 236 3.67 % 5586 86.75 % 498 7.73 % 359 5.58 % 258 4.01 %

PAR-RAPID 121 394 0.19 % 93 23.60 % 283 71.83 % 23 5.84 % 18 4.57 % 0 0.00 %

SER-FLEXI 24 768 0.37 % 43 5.60 % 609 79.30 % 69 8.98 % 51 6.64 % 65 8.46 %

SER-FRAUD 2 100 0.05 % 55 55.00 % 45 45.00 % 1 1.00 % 0 0.00 % 0 0.00 %

Powys PAR-HERD-S 14 658 0.32 % 73 11.09 % 546 82.98 % 47 7.14 % 38 5.78 % 1 0.15 %

PAR-NEW-IN 22 852 0.41 % 20 2.35 % 797 93.54 % 48 5.63 % 33 3.87 % 2 0.23 %

PAR-OTHER 5 109 0.05 % 3 2.75 % 103 94.50 % 4 3.67 % 3 2.75 % 0 0.00 %








PAR-PERSIS 43 3,626 1.75 % 129 3.56 % 3111 85.80 % 259 7.14 % 213 5.87 % 173 4.77 %

PAR-RAPID 31 111 0.05 % 14 12.61 % 85 76.58 % 12 10.81 % 11 9.91 % 1 0.90 %

SER-FLEXI 6 101 0.05 % 3 2.97 % 93 92.08 % 5 4.95 % 5 4.95 % 0 0.00 %

S Wales PAR-PERSIS 2 7 0.00 % 1 14.29 % 4 57.14 % 2 28.57 % 2 28.57 % 0 0.00 %

PAR-RAPID 3 8 0.00 % 2 25.00 % 6 75.00 % 0 0.00 % 0 0.00 % 0 0.00 %

W Glamorgan PAR-RAPID 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales - Intermediate TB Carms PAR-PERSIS 1 8 0.00 % 1 12.50 % 7 87.50 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-RAPID 2 11 0.01 % 4 36.36 % 7 63.64 % 0 0.00 % 0 0.00 % 0 0.00 %

Ceredigion PAR-HERD-S 8 891 0.43 % 6 0.67 % 722 81.03 % 216 24.24 % 163 18.29 % 0 0.00 %

PAR-NEW-IN 1 68 0.03 % 4 5.88 % 63 92.65 % 3 4.41 % 1 1.47 % 0 0.00 %

PAR-PERSIS 3 153 0.07 % 22 14.38 % 116 75.82 % 24 15.69 % 15 9.80 % 0 0.00 %

PAR-RAPID 19 86 0.04 % 20 23.26 % 63 73.26 % 4 4.65 % 3 3.49 % 0 0.00 %

SER-FLEXI 4 117 0.06 % 3 2.56 % 107 91.45 % 8 6.84 % 7 5.98 % 0 0.00 %

Mid glamorgan PAR-PERSIS 2 106 0.05 % 2 1.89 % 80 75.47 % 30 28.30 % 24 22.64 % 0 0.00 %

NE Wales PAR-HERD-S 1 17 0.01 % 0 0.00 % 13 76.47 % 4 23.53 % 4 23.53 % 0 0.00 %

PAR-NEW-IN 115 11,334 5.47 % 190 1.68 % 10435 92.07 % 871 7.68 % 651 5.74 % 58 0.51 %

PAR-PERSIS 1 101 0.05 % 3 2.97 % 91 90.10 % 8 7.92 % 7 6.93 % 0 0.00 %

PAR-RAPID 9 21 0.01 % 3 14.29 % 17 80.95 % 0 0.00 % 0 0.00 % 1 4.76 %

SER-FLEXI 4 82 0.04 % 7 8.54 % 71 86.59 % 5 6.10 % 4 4.88 % 0 0.00 %

Powys PAR-HERD-S 1 1 0.00 % 0 0.00 % 0 0.00 % 0 0.00 % 0 0.00 % 1 100.00 %

PAR-NEW-IN 8 398 0.19 % 15 3.77 % 364 91.46 % 38 9.55 % 19 4.77 % 0 0.00 %

PAR-RAPID 2 7 0.00 % 2 28.57 % 5 71.43 % 0 0.00 % 0 0.00 % 0 0.00 %

Shropshire PAR-NEW-IN 2 21 0.01 % 1 4.76 % 19 90.48 % 4 19.05 % 1 4.76 % 0 0.00 %

W Glamorgan PAR-OTHER 1 11 0.01 % 5 45.45 % 6 54.55 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 2 5 0.00 % 0 0.00 % 4 80.00 % 1 20.00 % 1 20.00 % 0 0.00 %

Wales - Low TB Gwynedd PAR-NEW-IN 14 867 0.42 % 8 0.92 % 786 90.66 % 99 11.42 % 72 8.30 % 1 0.12 %

PAR-RAPID 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

NE Wales PAR-NEW-IN 35 4,431 2.14 % 92 2.08 % 3930 88.69 % 334 7.54 % 251 5.66 % 158 3.57 %








PAR-OTHER 1 2 0.00 % 1 50.00 % 1 50.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 1 5 0.00 % 0 0.00 % 2 40.00 % 3 60.00 % 3 60.00 % 0 0.00 %

PAR-RAPID 3 14 0.01 % 2 14.29 % 12 85.71 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 2 46 0.02 % 0 0.00 % 45 97.83 % 2 4.35 % 1 2.17 % 0 0.00 %

Wales 940 45113 21.76 % 1950 4.32 % 39316 87.15 % 3725 8.26 % 2711 6.01 % 1136 2.52 %

Scotland - Scotland Aberdeenshire PAR-NEW-IN 2 15 0.01 % 0 0.00 % 14 93.33 % 1 6.67 % 1 6.67 % 0 0.00 %

Argyll & Bute PAR-NEW-IN 12 887 0.43 % 68 7.67 % 766 86.36 % 77 8.68 % 53 5.98 % 0 0.00 %

SER-FLEXI 1 10 0.00 % 1 10.00 % 8 80.00 % 1 10.00 % 1 10.00 % 0 0.00 %

Ayrshire PAR-NEW-IN 7 579 0.28 % 37 6.39 % 496 85.66 % 66 11.40 % 46 7.94 % 0 0.00 %

PAR-OTHER 1 46 0.02 % 39 84.78 % 7 15.22 % 1 2.17 % 0 0.00 % 0 0.00 %

Perthshire PAR-NEW-IN 3 217 0.10 % 17 7.83 % 187 86.18 % 22 10.14 % 13 5.99 % 0 0.00 %

West lothian PAR-NEW-IN 2 142 0.07 % 1 0.70 % 137 96.48 % 4 2.82 % 4 2.82 % 0 0.00 %

Wigtown PAR-NEW-IN 3 345 0.17 % 18 5.22 % 306 88.70 % 29 8.41 % 21 6.09 % 0 0.00 %

Scotland 31 2241 1.08 % 181 8.08 % 1921 85.72 % 201 8.97 % 139 6.20 % 0 0.00 %

Grand Total Sum: 2159 207362 9849 ###### 16682 11573 1923

*For test criteria please refer to Operational notes.




Table 3a. Summary of IFN Gamma results by country and protocol October 2018

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject

n % of total n % n % n % n % n %

England Parallel 151 18937 98.3% 1029 5.4% 16932 89.4% 1541 8.1% 966 5.1% 10 0.1%

Serial/Extended 6 325 1.7% 25 7.7% 291 89.5% 21 6.5% 8 2.5% 1 0.3%

Total 157 19262 100.0% 1054 5.5% 17223 89.4% 1562 8.1% 974 5.1% 11 0.1%

Scotland Parallel 2 15 100.0% 0 0.0 % 14 93.3% 1 6.7% 1 6.7% 0 0.0 %

Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Total 2 15 100.0% 0 0.0 % 14 93.3% 1 6.7% 1 6.7% 0 0.0 %

Wales Parallel 94 4205 89.5% 316 7.5% 3701 88.0% 270 6.4% 148 3.5% 40 1.0%

Serial/Extended 21 494 10.5% 47 9.5% 420 85.0% 27 5.5% 22 4.5% 5 1.0%

Total 115 4699 100.0% 363 7.7% 4121 87.7% 297 6.3% 170 3.6% 45 1.0%

GB Parallel 247 23157 96.6% 1345 5.8% 20647 89.2% 1812 7.8% 1115 4.8% 50 0.2%

Serial/Extended 27 819 3.4% 72 8.8% 711 86.8% 48 5.9% 30 3.7% 6 0.7%

Total 274 23976 100.0% 1417 5.9% 21358 89.1% 1860 7.8% 1145 4.8% 56 0.2%

157.00

115.00

2.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2018)

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject


England Parallel 1139 153933 96.2% 7274 4.7% 137402 89.3% 12353 8.0% 8482 5.5% 775 0.5%

Serial/Extended 49 6075 3.8% 444 7.3% 5378 88.5% 403 6.6% 241 4.0% 12 0.2%

Total 1188 160008 100.0% 7718 4.8% 142780 89.2% 12756 8.0% 8723 5.5% 787 0.5%

Scotland Parallel 30 2231 99.6% 180 8.1% 1913 85.7% 200 9.0% 138 6.2% 0 0.0 %

Serial/Extended 1 10 0.4% 1 0.0% 8 80.0% 1 10.0% 1 10.0% 0 0.0 %

Total 31 2241 100.0% 181 8.1% 1921 85.7% 201 9.0% 139 6.2% 0 0.0 %

Wales Parallel 872 43098 95.5% 1777 4.1% 37708 87.5% 3512 8.1% 2549 5.9% 1064 2.5%

Serial/Extended 68 2015 4.5% 173 8.6% 1608 79.8% 213 10.6% 162 8.0% 72 3.6%

Total 940 45113 100.0% 1950 4.3% 39316 87.2% 3725 8.3% 2711 6.0% 1136 2.5%

GB Parallel 2041 199262 96.1% 9231 4.6% 177023 88.8% 16065 8.1% 11169 5.6% 1839 0.9%

Serial/Extended 118 8100 3.9% 618 7.6% 6994 86.3% 617 7.6% 404 5.0% 84 1.0%

Total 2159 207362 100.0% 9849 4.7% 184017 88.7% 16682 8.0% 11573 5.6% 1923 0.9%

#######

940.00

31.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 26 809 3.4% 72 8.9% 701 86.7% 48 5.9 % 30 3.7 % 6 0.7 %

Parallel - Herd or Group Slaughter 7 423 1.8% 20 4.7% 392 92.7% 19 4.5 % 11 2.6 % 0

Parallel New Infection 109 11297 47.1% 560 5.0% 10112 89.5% 1016 9.0 % 619 5.5 % 6 0.1 %

Parallel - new infection cull area 37 4511 18.8% 267 5.9% 4007 88.8% 340 7.5 % 230 5.1 % 7 0.2 %

Parallel - Other 9 1235 5.2% 78 6.3% 1108 89.7% 66 5.3 % 48 3.9 % 1 0.1 %

Parallel Persistent Infection 42 4932 20.6% 366 7.4% 4384 88.9% 321 6.5 % 175 3.5 % 7 0.1 %

Parallel - persistent infection cull area 5 596 2.5% 19 3.2% 551 92.4% 41 6.9 % 26 4.4 % 0

Parallel - Rapid Testing Two-times IRs 38 163 0.7% 35 21.5% 93 57.1% 9 5.5 % 6 3.7 % 29 17.8 %

Serial - Suspected NSR Herd 1 10 0.0% 0 0.0 % 10 100.0% 0 0 0

Total 274 23976 100.0% 1417 5.9% 21358 89.1% 1860 7.8 % 1145 4.8 % 56 0.2 %

Table 4a. Summaryof IFN Gamma results by submission reason (October 2018)

England - HRA Extended Flexible IFN-GAMMA test 5 315 1.3% 25 7.9% 281 89.2% 21 6.7 % 8 2.5 % 1 0.3 %

Parallel New Infection 3 891 3.7% 62 7.0% 805 90.3% 44 4.9 % 24 2.7 % 0


Parallel - Other 7 1215 5.1% 71 5.8% 1095 90.1% 66 5.4 % 48 4.0 % 1 0.1 %

Parallel Persistent Infection 13 2421 10.1% 139 5.7% 2209 91.2% 146 6.0 % 73 3.0 % 0

Parallel - persistent infection cull area 5 596 2.5% 19 3.2% 551 92.4% 41 6.9 % 26 4.4 % 0

England - HRA 70 9949 41.5% 583 5.9% 8948 89.9% 658 6.6 % 409 4.1 % 9 0.1 %

England - Edge Parallel New Infection 78 8216 34.3% 429 5.2% 7267 88.4% 822 10.0 % 518 6.3 % 2 0.0 %

Parallel Persistent Infection 1 11 0.0% 0 0.0 % 8 72.7% 3 27.3 % 3 27.3 % 0


England - Edge 80 8237 34.4% 429 5.2% 7285 88.4% 825 10.0 % 521 6.3 % 2 0.0 %

England - LRA Parallel New Infection 7 1076 4.5% 42 3.9% 990 92.0% 79 7.3 % 44 4.1 % 0

England - LRA 7 1076 4.5% 42 3.9% 990 92.0% 79 7.3 % 44 4.1 % 0

Wales - High TB Extended Flexible IFN-GAMMA test 16 410 1.7% 40 9.8% 348 84.9% 21 5.1 % 17 4.1 % 5 1.2 %

Parallel - Herd or Group Slaughter 6 369 1.5% 20 5.4% 341 92.4% 13 3.5 % 8 2.2 % 0


Parallel - Other 1 9 0.0% 2 22.2% 7 77.8% 0 0 0



Wales - High TB 88 3503 14.6% 330 9.4% 3000 85.6% 219 6.3 % 130 3.7 % 43 1.2 %

Wales - Intermediate TB Extended Flexible IFN-GAMMA test 5 84 0.4% 7 8.3% 72 85.7% 6 7.1 % 5 6.0 % 0

Parallel - Herd or Group Slaughter 1 54 0.2% 0 0.0 % 51 94.4% 6 11.1 % 3 5.6 % 0


Parallel - Other 1 11 0.0% 5 45.5% 6 54.5% 0 0 0


Parallel - Rapid Testing Two-times IRs 3 9 0.0% 2 22.2% 7 77.8% 0 0 0

Wales - Intermediate TB 23 841 3.5% 29 3.4% 780 92.7% 59 7.0 % 32 3.8 % 0

Wales - Low TB Parallel New Infection 3 354 1.5% 4 1.1% 340 96.0% 19 5.4 % 8 2.3 % 2 0.6 %

Parallel - Rapid Testing Two-times IRs 1 1 0.0% 0 0.0 % 1 100.0% 0 0 0

Wales - Low TB 4 355 1.5% 4 1.1% 341 96.1% 19 5.4 % 8 2.3 % 2 0.6 %

Scotland - Scotland Parallel New Infection 2 15 0.1% 0 0.0 % 14 93.3% 1 6.7 % 1 6.7 % 0

Scotland - Scotland 2 15 0.1% 0 0.0 % 14 93.3% 1 6.7 % 1 6.7 % 0

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 104 7801 3.8% 560 7.2% 6761 86.7% 604 7.7 % 396 5.1 % 84 1.1 %

Parallel - Herd or Group Slaughter 89 5696 2.7% 224 3.9% 4711 82.7% 544 9.6 % 391 6.9 % 370 6.5 %



Parallel - Other 51 2585 1.2% 225 8.7% 2143 82.9% 147 5.7 % 108 4.2 % 109 4.2 %


Parallel - persistent infection cull area 32 6155 3.0% 339 5.5% 5428 88.2% 419 6.8 % 288 4.7 % 100 1.6 %


Serial - Anomalous Reaction 10 270 0.1% 58 21.5% 204 75.6% 13 4.8 % 8 3.0 % 0


Total 2159 207362 100.0% 9849 4.7% 184017 88.7% 16682 8.0 % 11573 5.6 % 1923 0.9 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2018)

England - HRA Extended Flexible IFN-GAMMA test 37 5876 2.8% 441 7.5% 5190 88.3% 391 6.7 % 233 4.0 % 12 0.2 %



Parallel - Other 27 2315 1.1% 161 7.0% 1943 83.9% 138 6.0 % 102 4.4 % 109 4.7 %


Parallel - persistent infection cull area 32 6155 3.0% 339 5.5% 5428 88.2% 419 6.8 % 288 4.7 % 100 1.6 %

Serial - Anomalous Reaction 8 170 0.1% 3 1.8% 159 93.5% 12 7.1 % 8 4.7 % 0

England - Edge Parallel New Infection 611 77650 37.4% 3164 4.1% 69852 90.0% 6520 8.4 % 4408 5.7 % 226 0.3 %

Parallel - Other 2 2 0.0% 0 0.0 % 1 50.0% 1 50.0 % 1 50.0 % 0



England - LRA Parallel New Infection 81 9008 4.3% 325 3.6% 8135 90.3% 780 8.7 % 545 6.1 % 3 0.0 %


England 1188 160008 77.2% 7718 4.8% 142780 89.2% 12756 8.0 % 8723 5.5 % 787 0.5 %

Wales - High TB Extended Flexible IFN-GAMMA test 54 1665 0.8% 108 6.5% 1336 80.2% 196 11.8 % 149 8.9 % 72 4.3 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2018) Cont.



Parallel - Other 19 209 0.1% 19 9.1% 185 88.5% 7 3.3 % 5 2.4 % 0



Serial - Anomalous Reaction 2 100 0.0% 55 55.0% 45 45.0% 1 1.0 % 0 0

Wales - Intermediate TB Extended Flexible IFN-GAMMA test 10 204 0.1% 10 4.9% 182 89.2% 14 6.9 % 12 5.9 % 0



Parallel - Other 1 11 0.0% 5 45.5% 6 54.5% 0 0 0



Wales - Low TB Extended Flexible IFN-GAMMA test 2 46 0.0% 0 0.0 % 45 97.8% 2 4.3 % 1 2.2 % 0


Parallel - Other 1 2 0.0% 1 50.0% 1 50.0% 0 0 0

Parallel Persistent Infection 1 5 0.0% 0 0.0 % 2 40.0% 3 60.0 % 3 60.0 % 0

Parallel - Rapid Testing Two-times IRs 4 15 0.0% 2 13.3% 13 86.7% 0 0 0

Wales 940 45113 21.8% 1950 4.3% 39316 87.2% 3725 8.3 % 2711 6.0 % 1136 2.5 %

Scotland - Scotland Extended Flexible IFN-GAMMA test 1 10 0.0% 1 10.0% 8 80.0% 1 10.0 % 1 10.0 % 0


Parallel - Other 1 46 0.0% 39 84.8% 7 15.2% 1 2.2 % 0 0

Scotland 31 2241 1.1% 181 8.1% 1921 85.7% 201 9.0 % 139 6.2 % 0

Table 5a. Summary of PM and culture results for IFNg positive animals.

Year Month Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

WrongEartags

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

2017 November 157 10666 108 961 2 24 0 0 0 0 87 3 0 8 0 800 0 31 1 0

December 114 9478 81 668 43 0 0 0 0 29 2 0 1 0 562 0 28 1 1

2018 January 207 19678 140 969 5 3 0 0 0 0 80 3 0 5 8 756 0 84 0 24

February 206 20791 130 1229 2 22 0 0 0 0 72 7 1 8 0 1075 1 35 0 5

March 189 17292 131 1199 1 3 0 0 0 0 121 6 0 4 0 987 0 72 1 2

April 195 20517 118 953 4 16 0 0 0 0 116 1 0 0 0 748 0 66 1 1

May 250 21066 131 947 1 2 0 0 0 0 121 1 0 4 0 781 0 35 1 1

June 187 18891 110 701 2 2 0 0 0 0 78 2 0 12 0 579 0 25 0 0

July 207 24111 119 806 1 12 0 0 0 0 67 0 3 2 1 675 3 36 0 4

August 228 19599 120 682 23 30 0 0 0 0 59 0 0 4 4 523 7 23 0 1

September 216 21441 137 946 6 15 0 0 0 0 33 0 3 0 0 833 56 0 0 0

October 274 23976 152 1417 479 464 0 0 0 0 19 0 0 0 0 195 5 0 0 0

Totals (last12 months) 2430 227506 1477 11478 526 636 0 0 0 0 882 25 7 48 13 8514 72 435 5 39

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Country Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

England 1303 175903 911 8828 478 0 0 0 0 752 13 5 29 2 6941 5 38 1 2

Scotland 37 3082 25 352 47 0 0 0 0 33 4 0 0 8 188 0 37 1 27

Wales 1090 48521 541 2298 111 0 0 0 0 97 8 2 19 3 1385 67 360 3 10

Totals 2430 227506 1477 11478 636 0 0 0 0 882 25 7 48 13 8514 72 435 5 39

Figure 4a. Number of herds sampled for IFNg and numbers of herds with anypositive IFNg results.

40

106

53

87

48

87

50

86

47

85

65

95

45

72

69

75

39

52

37

53

45

83

39

68

58

85

36

86

46

93

38

83

74

103

54

72

81

81

53

97

56

94

52

96

49

108

33

81

67

140

76

130

58

131

77

118

119

131

77

110

88

119

108

120

79

137

122

152

0

50

100

150

200

250

300

JanuaryFebruaryM

archAprilM

ayJuneJulyAugustSeptem

berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM


berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM


berO

ctober

2016

2017

2018

IFNg Positive herds IFNg Negative herds

7789

542

6091

549

7323

420

9568

453

9409

536

8947

398

10544

376

8010

424

7065

288

6507

334

8559

647

7448

325

9059

420

9793

442

10459

573

10179

906

13951

615

11775

419

11040

437

10097

560

10063

664

13635

971

9492

961

8778

66818401

969

19186

1229

15561

1199

18776

953

19795

947

17980

701

22955

806

18566

682

20202

946

22358

1416

0

5000

10000

15000

20000

25000

JanuaryFebruaryM

archAprilM


berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM


berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM


berO

ctober

2016

2017

2018

IFNg positive animals IFNg non positive animals*

Figure 4b. Number of animals sampled for IFNg and numbers of animals withpositive IFNg results.

*Includes all animals with IFNg test negative, resample and reject outcomes

0

1

2

3

4

5

6

7

8

Janu

ary

Febr

uary

Mar

chAp

rilM

ayJu

ne July

Augu

stSe

ptem

ber

Oct

ober

Nov

embe

rD

ecem

ber

Janu

ary

Febr

uary

Mar

chAp

rilM

ayJu

ne July

Augu

stSe

ptem

ber

Oct

ober

Nov

embe

rD

ecem

ber

Janu

ary

Febr

uary

Mar

chAp

rilM

ayJu

ne July

Augu

stSe

ptem

ber

Oct

ober

2016

2017

2018%Rejected %Resampled % NEG Fail % POS Fail

% of totalsubmitted

Figure 5. Rates of resamples including the fail rates broken down for positive(POS) and negative (NEG) controls.

0 20 40 60 80 100

120

W Sussex

W Glamorgan

Somerset

Powys

Oxon

Northants

Leics & Rut

Heref

Gwent

E Sussex

Devon

Cumbria

Cheshire

Carms

Berks

Aberdeenshire

%Retested %Resampled

Figure 6. Proportion of samples retested, or requiring a resample, during October 2018 (% submission perCounty) County %Resampled %Retested

Aberdeenshire 6.67 % 6.67 %

Avon 50.00 % 50.00 %

Berks 1.96 % 1.96 %

Bucks 8.33 % 12.50 %

Carms 4.05 % 5.86 %

Ceredigion 6.49 % 10.39 %

Cheshire 8.38 % 11.98 %

Corn & Scilly 6.53 % 8.73 %

Cumbria 5.38 % 8.80 %

Derbyshire 5.56 % 9.92 %

Devon 3.61 % 6.56 %

Dorset 5.55 % 7.27 %

E Sussex 5.95 % 8.33 %

Gloucs 3.97 % 8.22 %

Gwent 4.17 % 19.91 %

Hampshire

Heref 7.11 % 8.63 %

Kent 2.62 % 5.70 %

Leics & Rut 2.40 % 5.82 %

NE Wales 3.11 % 6.22 %

Northants 4.17 % 8.33 %

Notts 10.17 % 11.86 %

Oxon 5.73 % 8.82 %

Pembs 3.07 % 4.07 %

Powys 3.98 % 6.39 %

Shropshire 3.31 % 4.56 %

Somerset 3.62 % 4.71 %

Warks 2.19 % 3.95 %

W Glamorgan

Wiltshire 2.80 % 4.56 %

W Sussex 27.78 % 33.33 %

Country No.animals

No of IFHg+animals

% Positiveanimals No. samples No of IFHg+

samples% Positivesamples

England 19127 1054 5.5% 19262 1054 5.5%

Wales 4633 362 7.8% 4699 363 7.7%

Scotland 14 0 0.0 % 15 0.0 %

GB 23774 1416 6.0% 23976 1417 5.9%

Table 6a (i): Number of animals and submissions tested in October 2018

Country No.animals

No of IFHg+animals



England 140798 7718 5.5% 160008 7718 4.8%

Wales 39469 1949 4.9% 45113 1950 4.3%

Scotland 2080 181 8.7% 2241 181 8.1%

GB 182330 9848 5.4% 207362 9849 4.7%

Table 6b (i): Number of animals and submissions tested in 2018 Up to end of October

Country No.animals

No of IFHg+animals



England 12152 844 6.9% 12264 844 6.9%

Wales 2265 120 5.3% 2301 120 5.2%

Scotland 189 7 3.7% 189 7 3.7%

GB 14606 971 6.6% 14754 971 6.6%

Table 6a (ii): Number of animals and submissions tested in October 2017

Table 6b (ii): Number of animals and submissions tested in 2017 Up to end of October

Country No.animals

No of IFHg+animals



England 65172 3984 6.1% 82634 3984 4.8%

Wales 29318 1966 6.7% 32252 1966 6.1%

Scotland 2368 57 2.4% 2426 57 2.3%

GB 96855 6007 6.2% 117312 6007 5.1%

* Test type NumSubmissions Num Samples Num Positives

Private % Positive

Berkshire PRV-SP 1 2 0

Cheshire PRV-SP 1 1 1 100.00 %

Cornwall and Isles of Scilly PRV-SP 1 1 0

Devon PRV-SP 1 2 0

Dorset PRV-SE 1 2 0

PRV-SP 1 1 0

Hampshire PRV-SE 1 1 0

Total 7 10 1 10.00 %

*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;PRV-SP: Private High Specificity test – England

Table 7a Number of private samples October 2018

Table 7b Number of private samples 2018

* Test type NumSubmissions

NumSamples

NumPositivesPrivate

% Positive

Berkshire PRV-SP 1 2 0

Buckinghamshire PRV-SE 1 1 0

Cheshire PRV-SP 2 2 1 50.00 %

Cornwall and Isles of Scilly PRV-SE 1 3 0

PRV-SP 1 1 0

Devon PRV-SE 1 2 1 50.00 %

PRV-SP 1 2 0

Dorset PRV-SE 4 25 6 24.00 %

PRV-SP 1 1 0

East Sussex PRV-SE 1 1 0

PRV-SP 1 1 0

Hampshire PRV-SE 2 6 1 16.67 %

Northern Somerset & South Glou PRV-SE 2 46 8 17.39 %

Oxfordshire PRV-SE 1 38 4 10.53 %

Somerset excl North PRV-SE 3 46 3 6.52 %

PRV-SP 1 1 0

Suffolk PRV-SP 2 11 0

Total 26 189 24 12.70 %

Isle of Man SER-IOM 16 49 1 2.04 %

Isle of Man SER-IOM 2 4 1 25.00 %

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 South Wales 1 65 15 23.1 %TC0077 Sum: 65 15 23.1 %

Table 8a Number of Cattle Antibody Tests October 2018

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 Argyll and Bute 1 3 3 100.0 %Carmarthenshire 2 170 4 2.4 %Ceredigion 1 85 4 4.7 %Pembrokeshire 3 201 10 5.0 %Powys 1 345 3 0.9 %South Wales 5 199 17 8.5 %

TC0077 Sum: 1,003 41 4.1 %

Table 8b Number of Cattle Antibody Tests 2018

Documents

Monthly Report: Surveillance Project SB4008 IFNg tests for ...data.defra.gov.uk/Agriculture/APHA_Gamma monthly report - October 2018.pdf · SER-NSR 10 0.0 % Total 23976 100.00 % Figure