Monthly Report: Surveillance Project SB4008 IFNg tests for bovine … · 2020-02-05 · Serial test – other reasons NO (APHA Discretion) SER-FLEXI Extended blood test to provide

Monthly Report:Surveillance ProjectSB4008 IFNg tests forbovine tuberculosis

(TC0651 and TC0751)

Number 160

Report Period 1st - 31st December 2019

Issued 10 January 2020

Operational notes on terminology, definitions, re-test and re-samples testoutcomes:

• “Samples” refers to an individual tube of blood taken from an individual animal which issubsequently submitted for testing. It is generally assumed that the number of samples andthe number of animals are analogous as any duplicate sample blood tube from the sameanimal will not be tested. However, minor discrepancies may occur where the same animal istested more than once due to a request for a resample or the animal is tested twice underdifferent categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).

• “Submissions” refers to an individual batch or set of samples received for testing.Submissions may comprise 1 or more samples (for example, if an entire herd is being tested itis usually sent as 1 submission). It is generally assumed that the number of submissions andthe number of herds are analogous.

• Retests are samples where the first ELISA assay fails and the same sample is retested on anew ELISA plate. Each sample can be retested only once. A retest is not a reportable testoutcome.

• Resamples are where a sample has been retested and failed a second time, so that the labrequests a new sample. This is a reportable test outcome.

• Rejects are samples that are not tested by the lab for one of the following reasons: bloodcollected into wrong type of vacutainer, samples that have not been maintained at theappropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, bloodthat is extensively clotted (small clots are OK), samples received after 4pm on the day aftersample collection. Such samples are reported separately in the tables and in figure 5.

• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure ofthe quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) mayindicate compromised blood quality as a result of collection/transportation conditions or duethe animal having an unusually low/suppressed cellular immune response.

• NEG (no-antigen control) is a sample negative control which provides a measure of thebackground antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)may indicate a laboratory procedures problem (normally resolved during re-test) or that ananimal has unusually high background levels of IFN-g.

Submission Reason Explanation Mandatory?

Possible Herd Slaughter(PAR-HERD-S)

Parallel interferon-gamma blood testing of skintest negative cattle to inform whole or partialherd slaughters decisions

YES, if APHA arecontemplating a herdslaughter

Persistent TB breakdowns(OTF status withdrawn)(PAR-P-CONF) N.B.PAR-PERSIS from 1st April2017

Parallel blood testing of skin test-negative cattlein persistently infected herds that have failed toresolve by repeated short-interval skin testingand fulfill a minimum of biosecurity standards.

NO (APHA discretion)

Parallel - Low Incidence(PAR-LOW-IN) N.B.PAR-NEW-IN from 1st April2017

Parallel blood testing to maximise theprobability of removing all infected cattle in anew herd breakdown as soon as possible afterconfirmation of TB.

YES in Area of LowIncidence

Rapid Testing of twice IR's(PAR-RAPID)

Parallel blood testing of two-times IRs identifiedunder the severe interpretation of the skin testused in Wales.

YES (Wales only)

PAR-CUL-N

Parallel blood testing to maximise theprobability of removing all infected cattle in anew breakdown as soon as possible afterconfirmation of TB where herd is situated inbadger control areas of England that havecompleted at least 2 effective culls.

YES (in eligible badgercontrol areas of England)

PAR-CUL-P

Parallel blood testing to maximise theprobability of removing all infected cattle in apersistent confirmed breakdown where herd issituated within badger control areas of Englandthat have completed at least 2 effective culls


Parallel Other(PAR-OTHER)

Other parallel blood testing not covered in anyof the other scenarios NO (APHA discretion)

'NSR' Herds (SER-NSR)

Modified serial blood testing of individual skintest reactors and/or IRs in unconfirmed TBbreakdown herds to clarify their infection statuswhere there is evidence of non-specificsensitisation to bovine tuberculin (the“non-specific reactor” procedure)


Suspected Fraud(SER-FRAUD)

Modified serial blood test of suspectedfraudulent reactors to the skin test (animals withabnormal skin swellings), in confirmed orunconfirmed TB incidents.

NO (APHA discretion tofirm up or rule out anysuspicion of fraud andsupport anyinvestigations)

Serial Other(SER-OTHER) Serial test – other reasons NO (APHA Discretion)

SER-FLEXI

Extended blood test to provide flexible testreadout in a confirmed M. bovis-infected herdwhere Johne's (M. a. paratuberculosis) infectionor vaccination is suspected to be interferringwith M. bovis infection detection


Test Criteria

NumsamplesDecember2019

% Total 2019 %

Submissions 234 3471

Samples Total (%) 25192 100.00 % 331966 100.00 %

England 19595 77.78 % 263774 79.46 %

Wales 5597 22.22 % 65499 19.73 %

Scotland 2693 0.81 %

Parallel Tests PAR-CUL-N 9518 37.78 % 75646 22.79 %

PAR-CUL-P 400 1.59 % 21742 6.55 %

PAR-HERD-S 851 3.38 % 8385 2.53 %

PAR-NEW-IN 7512 29.82 % 147126 44.32 %

PAR-OTHER 578 2.29 % 5794 1.75 %

PAR-PERSIS 5998 23.81 % 65152 19.63 %

PAR-RAPID 127 0.50 % 1512 0.46 %

Total(% of all samples) 24984 99.17 % 325357 98.01 %

Serial/Extended Tests SER-FLEXI 208 0.83 % 6571 1.98 %

SER-FRAUD 0 0.00 % 27 0.01 %

SER-NSR 0 0.00 % 11 0.00 %

Total(% of all samples) 208 0.83 % 6609 1.99 %

Total 25192 100.00 % 331966 100.00 %

Retests Total (% of all samples) 1500 5.95 % 22682 6.83 %

England 1202 4.77 % 18623 5.61 %

Wales 298 1.18 % 3932 1.18 %

Scotland 127 0.04 %

Resamples Total (% of all samples) 1197 4.75 % 17635 5.31 %

England 987 3.92 % 14547 4.38 %

Wales 210 0.83 % 2984 0.90 %

Scotland 104 0.03 %

Rejects Total (% of all samples) 1166 4.63 % 5314 1.60 %

England 1159 4.60 % 4726 1.42 %

Wales 7 0.03 % 578 0.17 %

Scotland 0 0.00 % 10 0.00 %

Table 1. Monthly Totals for December 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

PAR-CUL-N

PAR-CUL-P

PAR-HERD-S

PAR-NEW-IN

PAR-OTHER

PAR-PERSIS

PAR-RAPIDSER-FLEXI

Breakdown ofReason forsubmission

PAR-CUL-N 9518 37.8 %

PAR-CUL-P 400 1.6 %

PAR-HERD-S 851 3.4 %

PAR-NEW-IN 7512 29.8 %

PAR-OTHER 578 2.3 %

PAR-PERSIS 5998 23.8 %

PAR-RAPID 127 0.5 %

SER-FLEXI 208 0.8 %

Total 25192 100.00 %

Figure 1. Number of samples submitted in each category during December 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

0

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

W Glamorgan

S Wales

S Glamorgan

Powys

Pembs

NE Wales

Mid Glamorgan

Gwynedd

Gwent

Ceredigion

Carms

Wiltshire

Warks

Somerset excl N

Oxon

N Yorks

Northants

Leics & Rut

Heref

Gr Manchester

Gloucs

E Sussex

Dorset

Devon

Derbyshire

Corn & Scilly

Cheshire

Bucks

Avon

Wales

England

SER-FLEXI

PAR-RAPID

PAR-PERSIS

PAR-OTHER

PAR-NEW-IN

PAR-HERD-S

PAR-CUL-P

PAR-CUL-N

Breakdown ofSamples

submitted byCounty

Figure 2. Number of samples submitted in each category by County during December 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallelother, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serialpotential fraud, SER-OTHER: serial other

0

1000

0

2000

0

3000

0

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0

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WigtownKirkcudbrightDumfries & G

AyrshireArgyll & ButeW Glamorgan

S WalesShropshire

S GlamorganPowysPembs

NE WalesMid Glamorgan

GwyneddGwent

CeredigionCarms

W YorksW Sussex

WorcsWiltshire

WarksS YorksSuffolkStaffs

Somerset excl NShropshire

OxonN Yorks

NottsNorthumbNorthants

NorfolkLincs

Leics & RutLancashire

KentI of Wight

HertsHeref

HampshireGr Manchester

Gr LondonGloucs

E SussexEssex

DorsetDevon

DerbyshireCumbria

Corn & ScillyCheshire

CambsBucksBerksAvon

Scotland

Wales

England

SER-NSR SER-FRAUD SER-FLEXI PAR-RAPID PAR-PERSIS PAR-OTHER PAR-NEW-IN PAR-HERD-S

PAR-CUL-P PAR-CUL-N

Breakdownof Samplessubmittedby County

Figure 3. Number of samples submitted in each category by County during 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrentJohne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country County SubmissionReasons*

No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject

n % of total n % n % n %

England - HRA Avon PAR-OTHER 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Corn & Scilly PAR-CUL-N 4 618 2.45 % 7 1.13 % 586 94.82 % 27 4.37 % 25 4.05 % 0 0.00 %

PAR-CUL-P 1 105 0.42 % 3 2.86 % 97 92.38 % 7 6.67 % 5 4.76 % 0 0.00 %

PAR-PERSIS 4 1,702 6.76 % 71 4.17 % 1450 85.19 % 80 4.70 % 63 3.70 % 118 6.93 %

Devon PAR-CUL-N 18 2,131 8.46 % 89 4.18 % 1809 84.89 % 212 9.95 % 186 8.73 % 47 2.21 %

PAR-PERSIS 3 405 1.61 % 13 3.21 % 378 93.33 % 18 4.44 % 14 3.46 % 0 0.00 %

Dorset PAR-CUL-N 7 1,670 6.63 % 22 1.32 % 1587 95.03 % 70 4.19 % 60 3.59 % 1 0.06 %

PAR-CUL-P 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %

SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 1 33.33 % 0 0.00 % 0 0.00 %

Gloucs PAR-CUL-N 4 195 0.77 % 4 2.05 % 181 92.82 % 3 1.54 % 3 1.54 % 7 3.59 %

PAR-CUL-P 3 282 1.12 % 11 3.90 % 260 92.20 % 16 5.67 % 11 3.90 % 0 0.00 %

Heref PAR-CUL-N 2 173 0.69 % 6 3.47 % 157 90.75 % 13 7.51 % 10 5.78 % 0 0.00 %

PAR-NEW-IN 1 30 0.12 % 8 26.67 % 21 70.00 % 1 3.33 % 1 3.33 % 0 0.00 %

PAR-PERSIS 2 196 0.78 % 7 3.57 % 174 88.78 % 17 8.67 % 15 7.65 % 0 0.00 %

Somerset excl N PAR-CUL-N 3 120 0.48 % 1 0.83 % 114 95.00 % 6 5.00 % 5 4.17 % 0 0.00 %

Wiltshire PAR-CUL-N 9 1,960 7.78 % 50 2.55 % 1491 76.07 % 79 4.03 % 62 3.16 % 357 18.21 %

PAR-CUL-P 1 11 0.04 % 1 9.09 % 7 63.64 % 3 27.27 % 3 27.27 % 0 0.00 %

PAR-OTHER 1 10 0.04 % 1 10.00 % 9 90.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 4 462 1.83 % 19 4.11 % 432 93.51 % 14 3.03 % 11 2.38 % 0 0.00 %

SER-FLEXI 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %

England - Edge Bucks PAR-NEW-IN 3 20 0.08 % 1 5.00 % 19 95.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Cheshire PAR-CUL-N 5 2,651 10.52 % 71 2.68 % 2402 90.61 % 214 8.07 % 178 6.71 % 0 0.00 %

PAR-NEW-IN 17 1,875 7.44 % 39 2.08 % 1698 90.56 % 154 8.21 % 128 6.83 % 10 0.53 %

PAR-PERSIS 1 32 0.13 % 3 9.38 % 20 62.50 % 9 28.13 % 9 28.13 % 0 0.00 %

Derbyshire PAR-NEW-IN 17 1,642 6.52 % 49 2.98 % 1383 84.23 % 74 4.51 % 59 3.59 % 151 9.20 %

E Sussex PAR-NEW-IN 1 140 0.56 % 11 7.86 % 76 54.29 % 7 5.00 % 3 2.14 % 50 35.71 %

Table 2a. Summary by County for December 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapidtesting of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)

Table 2a. Summary by County for December 2019 Cont.




Leics & Rut PAR-NEW-IN 4 407 1.62 % 20 4.91 % 381 93.61 % 9 2.21 % 6 1.47 % 0 0.00 %

Northants PAR-NEW-IN 6 1,468 5.83 % 58 3.95 % 966 65.80 % 91 6.20 % 76 5.18 % 368 25.07 %

Oxon PAR-NEW-IN 10 932 3.70 % 32 3.43 % 813 87.23 % 54 5.79 % 37 3.97 % 50 5.36 %

PAR-OTHER 1 111 0.44 % 7 6.31 % 102 91.89 % 3 2.70 % 2 1.80 % 0 0.00 %

Warks PAR-NEW-IN 4 117 0.46 % 17 14.53 % 95 81.20 % 7 5.98 % 5 4.27 % 0 0.00 %

England - LRA Gr Manchester PAR-NEW-IN 1 85 0.34 % 1 1.18 % 77 90.59 % 8 9.41 % 7 8.24 % 0 0.00 %

N Yorks PAR-NEW-IN 2 38 0.15 % 0 0.00 % 37 97.37 % 3 7.89 % 1 2.63 % 0 0.00 %

England 143 19595 77.78 % 623 3.18 % 16826 85.87 % 1202 6.13 % 987 5.04 % 1159 5.91 %

Wales - High TB Carms PAR-HERD-S 4 395 1.57 % 14 3.54 % 374 94.68 % 11 2.78 % 7 1.77 % 0 0.00 %

PAR-PERSIS 6 284 1.13 % 34 11.97 % 245 86.27 % 6 2.11 % 5 1.76 % 0 0.00 %

PAR-RAPID 5 24 0.10 % 5 20.83 % 16 66.67 % 3 12.50 % 3 12.50 % 0 0.00 %

Ceredigion PAR-PERSIS 1 213 0.85 % 9 4.23 % 202 94.84 % 5 2.35 % 2 0.94 % 0 0.00 %

PAR-RAPID 6 43 0.17 % 7 16.28 % 35 81.40 % 1 2.33 % 1 2.33 % 0 0.00 %

Gwent PAR-PERSIS 4 273 1.08 % 20 7.33 % 242 88.64 % 15 5.49 % 8 2.93 % 3 1.10 %

SER-FLEXI 2 58 0.23 % 15 25.86 % 42 72.41 % 1 1.72 % 1 1.72 % 0 0.00 %

Pembs PAR-HERD-S 1 25 0.10 % 2 8.00 % 23 92.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-OTHER 3 447 1.77 % 15 3.36 % 416 93.06 % 19 4.25 % 15 3.36 % 1 0.22 %

PAR-PERSIS 11 1,440 5.72 % 46 3.19 % 1349 93.68 % 67 4.65 % 45 3.13 % 0 0.00 %

PAR-RAPID 7 27 0.11 % 8 29.63 % 18 66.67 % 1 3.70 % 1 3.70 % 0 0.00 %

SER-FLEXI 3 82 0.33 % 9 10.98 % 72 87.80 % 2 2.44 % 1 1.22 % 0 0.00 %

Powys PAR-HERD-S 2 420 1.67 % 16 3.81 % 392 93.33 % 21 5.00 % 12 2.86 % 0 0.00 %

PAR-PERSIS 7 875 3.47 % 23 2.63 % 781 89.26 % 84 9.60 % 68 7.77 % 3 0.34 %

PAR-RAPID 2 21 0.08 % 3 14.29 % 17 80.95 % 1 4.76 % 1 4.76 % 0 0.00 %

S Wales PAR-HERD-S 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

W Glamorgan PAR-PERSIS 1 53 0.21 % 1 1.89 % 49 92.45 % 8 15.09 % 3 5.66 % 0 0.00 %

Wales - Intermediate TB Carms PAR-HERD-S 1 9 0.04 % 0 0.00 % 8 88.89 % 1 11.11 % 1 11.11 % 0 0.00 %

PAR-PERSIS 2 61 0.24 % 2 3.28 % 57 93.44 % 2 3.28 % 2 3.28 % 0 0.00 %




Table 2a. Summary by County for December 2019 Cont.




PAR-RAPID 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %

Mid Glamorgan PAR-RAPID 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

NE Wales PAR-NEW-IN 7 508 2.02 % 35 6.89 % 456 89.76 % 27 5.31 % 17 3.35 % 0 0.00 %

PAR-OTHER 3 9 0.04 % 0 0.00 % 7 77.78 % 4 44.44 % 2 22.22 % 0 0.00 %

PAR-PERSIS 1 2 0.01 % 1 50.00 % 1 50.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-RAPID 2 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 1 12 0.05 % 3 25.00 % 9 75.00 % 0 0.00 % 0 0.00 % 0 0.00 %

S Glamorgan SER-FLEXI 1 52 0.21 % 2 3.85 % 50 96.15 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales - Low TB Gwynedd PAR-NEW-IN 2 49 0.19 % 0 0.00 % 46 93.88 % 5 10.20 % 3 6.12 % 0 0.00 %


Wales 91 5597 22.22 % 281 5.02 % 5099 91.10 % 298 5.32 % 210 3.75 % 7 0.13 %

Grand Total Sum: 234 25192 904 21925 1500 1197 1166

*For test criteria please refer to Operational notes.







England - HRA Avon PAR-CUL-P 3 431 0.13 % 9 2.09 % 407 94.43 % 23 5.34 % 15 3.48 % 0 0.00 %

PAR-OTHER 5 84 0.03 % 1 1.19 % 71 84.52 % 12 14.29 % 12 14.29 % 0 0.00 %

PAR-PERSIS 9 1,490 0.45 % 45 3.02 % 1387 93.09 % 72 4.83 % 56 3.76 % 2 0.13 %

SER-FRAUD 3 27 0.01 % 6 22.22 % 19 70.37 % 2 7.41 % 2 7.41 % 0 0.00 %

Corn & Scilly PAR-CUL-N 51 5,213 1.57 % 158 3.03 % 4695 90.06 % 379 7.27 % 309 5.93 % 51 0.98 %

PAR-CUL-P 13 3,310 1.00 % 49 1.48 % 3064 92.57 % 252 7.61 % 193 5.83 % 4 0.12 %

PAR-NEW-IN 7 793 0.24 % 14 1.77 % 699 88.15 % 103 12.99 % 80 10.09 % 0 0.00 %

PAR-PERSIS 41 9,947 3.00 % 408 4.10 % 8700 87.46 % 532 5.35 % 426 4.28 % 413 4.15 %

SER-FLEXI 4 43 0.01 % 0 0.00 % 41 95.35 % 2 4.65 % 2 4.65 % 0 0.00 %

Devon PAR-CUL-N 236 30,357 9.14 % 1446 4.76 % 26813 88.33 % 2156 7.10 % 1641 5.41 % 457 1.51 %

PAR-CUL-P 30 7,658 2.31 % 342 4.47 % 6899 90.09 % 514 6.71 % 413 5.39 % 4 0.05 %

PAR-NEW-IN 4 674 0.20 % 106 15.73 % 501 74.33 % 86 12.76 % 67 9.94 % 0 0.00 %

PAR-OTHER 7 532 0.16 % 6 1.13 % 352 66.17 % 202 37.97 % 174 32.71 % 0 0.00 %

PAR-PERSIS 28 3,645 1.10 % 227 6.23 % 3206 87.96 % 281 7.71 % 211 5.79 % 1 0.03 %

SER-FLEXI 8 849 0.26 % 50 5.89 % 732 86.22 % 47 5.54 % 22 2.59 % 45 5.30 %

Dorset PAR-CUL-N 106 14,444 4.35 % 674 4.67 % 12978 89.85 % 950 6.58 % 735 5.09 % 57 0.39 %

PAR-CUL-P 20 3,391 1.02 % 254 7.49 % 3019 89.03 % 160 4.72 % 118 3.48 % 0 0.00 %

PAR-NEW-IN 5 55 0.02 % 1 1.82 % 43 78.18 % 11 20.00 % 11 20.00 % 0 0.00 %

SER-FLEXI 6 387 0.12 % 21 5.43 % 353 91.21 % 16 4.13 % 13 3.36 % 0 0.00 %

Gloucs PAR-CUL-N 63 4,539 1.37 % 169 3.72 % 3915 86.25 % 401 8.83 % 336 7.40 % 119 2.62 %

PAR-CUL-P 13 1,560 0.47 % 63 4.04 % 1375 88.14 % 156 10.00 % 122 7.82 % 0 0.00 %

PAR-HERD-S 4 33 0.01 % 0 0.00 % 31 93.94 % 3 9.09 % 2 6.06 % 0 0.00 %

PAR-NEW-IN 1 112 0.03 % 3 2.68 % 103 91.96 % 7 6.25 % 6 5.36 % 0 0.00 %

SER-FLEXI 2 30 0.01 % 2 6.67 % 28 93.33 % 2 6.67 % 0 0.00 % 0 0.00 %

Heref PAR-CUL-N 17 1,897 0.57 % 75 3.95 % 1698 89.51 % 142 7.49 % 113 5.96 % 11 0.58 %

PAR-CUL-P 4 418 0.13 % 16 3.83 % 373 89.23 % 36 8.61 % 29 6.94 % 0 0.00 %

Table 2b. Summary by County for 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other



Table 2b. Summary by County for 2019 Cont.




PAR-NEW-IN 3 43 0.01 % 8 18.60 % 33 76.74 % 5 11.63 % 2 4.65 % 0 0.00 %

PAR-OTHER 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 35 3,700 1.11 % 151 4.08 % 3319 89.70 % 274 7.41 % 218 5.89 % 12 0.32 %

Shropshire PAR-CUL-P 1 9 0.00 % 1 11.11 % 8 88.89 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-NEW-IN 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-OTHER 7 635 0.19 % 43 6.77 % 545 85.83 % 60 9.45 % 46 7.24 % 1 0.16 %

PAR-PERSIS 12 1,320 0.40 % 32 2.42 % 1167 88.41 % 138 10.45 % 120 9.09 % 1 0.08 %

SER-FLEXI 5 557 0.17 % 21 3.77 % 509 91.38 % 34 6.10 % 27 4.85 % 0 0.00 %

Somerset excl N PAR-CUL-N 51 5,778 1.74 % 230 3.98 % 4771 82.57 % 369 6.39 % 246 4.26 % 531 9.19 %

PAR-OTHER 2 238 0.07 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %

PAR-PERSIS 13 1,907 0.57 % 110 5.77 % 1510 79.18 % 105 5.51 % 69 3.62 % 218 11.43 %

Staffs PAR-OTHER 2 140 0.04 % 3 2.14 % 125 89.29 % 15 10.71 % 12 8.57 % 0 0.00 %

PAR-PERSIS 7 1,492 0.45 % 202 13.54 % 1201 80.50 % 130 8.71 % 90 6.03 % 0 0.00 %

Wiltshire PAR-CUL-N 78 9,058 2.73 % 301 3.32 % 7859 86.76 % 606 6.69 % 508 5.61 % 390 4.31 %

PAR-CUL-P 27 4,613 1.39 % 297 6.44 % 4119 89.29 % 286 6.20 % 194 4.21 % 3 0.07 %

PAR-NEW-IN 7 456 0.14 % 15 3.29 % 398 87.28 % 53 11.62 % 43 9.43 % 0 0.00 %

PAR-OTHER 5 607 0.18 % 6 0.99 % 591 97.36 % 19 3.13 % 10 1.65 % 0 0.00 %

PAR-PERSIS 19 4,037 1.22 % 179 4.43 % 3606 89.32 % 209 5.18 % 140 3.47 % 112 2.77 %

SER-FLEXI 4 71 0.02 % 9 12.68 % 59 83.10 % 4 5.63 % 3 4.23 % 0 0.00 %

Worcs PAR-PERSIS 4 138 0.04 % 6 4.35 % 99 71.74 % 2 1.45 % 1 0.72 % 32 23.19 %

England - Edge Berks PAR-NEW-IN 39 4,468 1.35 % 155 3.47 % 4016 89.88 % 362 8.10 % 297 6.65 % 0 0.00 %

PAR-PERSIS 3 204 0.06 % 5 2.45 % 196 96.08 % 5 2.45 % 3 1.47 % 0 0.00 %

Bucks PAR-NEW-IN 37 3,072 0.93 % 100 3.26 % 2717 88.44 % 270 8.79 % 220 7.16 % 35 1.14 %

Cheshire PAR-CUL-N 10 4,360 1.31 % 114 2.61 % 3992 91.56 % 308 7.06 % 254 5.83 % 0 0.00 %

PAR-NEW-IN 242 48,089 14.49 % 1056 2.20 % 44290 92.10 % 2824 5.87 % 2091 4.35 % 652 1.36 %

PAR-PERSIS 6 572 0.17 % 6 1.05 % 532 93.01 % 38 6.64 % 34 5.94 % 0 0.00 %

Derbyshire PAR-NEW-IN 191 21,184 6.38 % 659 3.11 % 18762 88.57 % 1600 7.55 % 1322 6.24 % 441 2.08 %

E Sussex PAR-NEW-IN 22 2,861 0.86 % 136 4.75 % 2526 88.29 % 164 5.73 % 152 5.31 % 63 2.20 %








Hampshire PAR-NEW-IN 37 3,029 0.91 % 71 2.34 % 2744 90.59 % 270 8.91 % 216 7.13 % 0 0.00 %

PAR-PERSIS 1 37 0.01 % 2 5.41 % 28 75.68 % 9 24.32 % 7 18.92 % 0 0.00 %

SER-NSR 2 8 0.00 % 0 0.00 % 8 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Leics & Rut PAR-NEW-IN 62 7,032 2.12 % 314 4.47 % 6243 88.78 % 472 6.71 % 380 5.40 % 95 1.35 %

PAR-PERSIS 1 336 0.10 % 20 5.95 % 305 90.77 % 11 3.27 % 8 2.38 % 3 0.89 %

SER-NSR 1 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Northants PAR-NEW-IN 37 3,690 1.11 % 181 4.91 % 2898 78.54 % 232 6.29 % 166 4.50 % 445 12.06 %

Notts PAR-NEW-IN 7 774 0.23 % 36 4.65 % 618 79.84 % 69 8.91 % 56 7.24 % 64 8.27 %

Oxon PAR-NEW-IN 164 16,269 4.90 % 643 3.95 % 14391 88.46 % 1307 8.03 % 1030 6.33 % 205 1.26 %

PAR-OTHER 1 111 0.03 % 7 6.31 % 102 91.89 % 3 2.70 % 2 1.80 % 0 0.00 %

PAR-PERSIS 2 339 0.10 % 5 1.47 % 312 92.04 % 28 8.26 % 22 6.49 % 0 0.00 %

Warks PAR-NEW-IN 112 9,238 2.78 % 413 4.47 % 8216 88.94 % 665 7.20 % 522 5.65 % 87 0.94 %

PAR-PERSIS 6 570 0.17 % 19 3.33 % 526 92.28 % 32 5.61 % 25 4.39 % 0 0.00 %

England - LRA Cambs PAR-NEW-IN 7 409 0.12 % 22 5.38 % 355 86.80 % 30 7.33 % 24 5.87 % 8 1.96 %

Cumbria PAR-NEW-IN 21 3,783 1.14 % 110 2.91 % 3430 90.67 % 325 8.59 % 243 6.42 % 0 0.00 %

PAR-PERSIS 1 135 0.04 % 4 2.96 % 121 89.63 % 19 14.07 % 10 7.41 % 0 0.00 %

Essex PAR-NEW-IN 2 94 0.03 % 1 1.06 % 91 96.81 % 2 2.13 % 2 2.13 % 0 0.00 %

Gr London PAR-NEW-IN 2 45 0.01 % 0 0.00 % 42 93.33 % 6 13.33 % 3 6.67 % 0 0.00 %

Gr Manchester PAR-NEW-IN 3 164 0.05 % 7 4.27 % 129 78.66 % 33 20.12 % 28 17.07 % 0 0.00 %

Herts PAR-NEW-IN 7 1,011 0.30 % 49 4.85 % 886 87.64 % 90 8.90 % 76 7.52 % 0 0.00 %

I of Wight PAR-NEW-IN 4 641 0.19 % 16 2.50 % 562 87.68 % 69 10.76 % 62 9.67 % 1 0.16 %

Kent PAR-NEW-IN 1 10 0.00 % 1 10.00 % 9 90.00 % 3 30.00 % 0 0.00 % 0 0.00 %

Lancashire PAR-NEW-IN 3 106 0.03 % 1 0.94 % 101 95.28 % 5 4.72 % 4 3.77 % 0 0.00 %

Lincs PAR-NEW-IN 12 1,350 0.41 % 33 2.44 % 1202 89.04 % 128 9.48 % 115 8.52 % 0 0.00 %

Norfolk PAR-NEW-IN 1 41 0.01 % 1 2.44 % 38 92.68 % 2 4.88 % 2 4.88 % 0 0.00 %

Northumb PAR-NEW-IN 3 296 0.09 % 0 0.00 % 241 81.42 % 63 21.28 % 55 18.58 % 0 0.00 %

PAR-PERSIS 3 183 0.06 % 1 0.55 % 32 17.49 % 58 31.69 % 57 31.15 % 93 50.82 %

N Yorks PAR-NEW-IN 9 913 0.28 % 22 2.41 % 803 87.95 % 74 8.11 % 60 6.57 % 28 3.07 %








PAR-PERSIS 3 575 0.17 % 2 0.35 % 551 95.83 % 31 5.39 % 22 3.83 % 0 0.00 %

SER-FLEXI 2 50 0.02 % 1 2.00 % 48 96.00 % 3 6.00 % 1 2.00 % 0 0.00 %

Suffolk PAR-NEW-IN 3 334 0.10 % 3 0.90 % 291 87.13 % 45 13.47 % 40 11.98 % 0 0.00 %

S Yorks PAR-NEW-IN 5 404 0.12 % 7 1.73 % 381 94.31 % 21 5.20 % 15 3.71 % 1 0.25 %

W Sussex PAR-NEW-IN 2 239 0.07 % 2 0.84 % 163 68.20 % 81 33.89 % 74 30.96 % 0 0.00 %

W Yorks PAR-HERD-S 2 24 0.01 % 8 33.33 % 14 58.33 % 2 8.33 % 2 8.33 % 0 0.00 %

England 2054 263774 79.46 % 10026 3.80 % 234494 88.90 % 18623 7.06 % 14547 5.51 % 4726 1.79 %

Wales - High TB Carms PAR-HERD-S 63 3,721 1.12 % 248 6.66 % 3283 88.23 % 248 6.66 % 188 5.05 % 2 0.05 %

PAR-NEW-IN 3 121 0.04 % 22 18.18 % 90 74.38 % 11 9.09 % 9 7.44 % 0 0.00 %

PAR-OTHER 16 464 0.14 % 70 15.09 % 384 82.76 % 20 4.31 % 10 2.16 % 0 0.00 %

PAR-PERSIS 79 6,894 2.08 % 393 5.70 % 6240 90.51 % 337 4.89 % 250 3.63 % 13 0.19 %

PAR-RAPID 87 389 0.12 % 96 24.68 % 272 69.92 % 34 8.74 % 21 5.40 % 0 0.00 %

SER-FLEXI 43 908 0.27 % 92 10.13 % 775 85.35 % 51 5.62 % 41 4.52 % 0 0.00 %

Ceredigion PAR-HERD-S 2 143 0.04 % 12 8.39 % 119 83.22 % 15 10.49 % 12 8.39 % 0 0.00 %

PAR-NEW-IN 2 313 0.09 % 14 4.47 % 289 92.33 % 10 3.19 % 10 3.19 % 0 0.00 %

PAR-OTHER 3 160 0.05 % 5 3.13 % 148 92.50 % 10 6.25 % 7 4.38 % 0 0.00 %

PAR-PERSIS 36 2,780 0.84 % 162 5.83 % 2477 89.10 % 189 6.80 % 140 5.04 % 1 0.04 %

PAR-RAPID 57 172 0.05 % 33 19.19 % 134 77.91 % 7 4.07 % 5 2.91 % 0 0.00 %

SER-FLEXI 10 533 0.16 % 18 3.38 % 501 94.00 % 15 2.81 % 12 2.25 % 2 0.38 %

Gwent PAR-HERD-S 11 887 0.27 % 50 5.64 % 760 85.68 % 84 9.47 % 75 8.46 % 2 0.23 %

PAR-OTHER 3 30 0.01 % 6 20.00 % 24 80.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 24 1,684 0.51 % 96 5.70 % 1513 89.85 % 99 5.88 % 71 4.22 % 4 0.24 %

PAR-RAPID 28 83 0.03 % 19 22.89 % 55 66.27 % 9 10.84 % 8 9.64 % 1 1.20 %

SER-FLEXI 4 82 0.02 % 17 20.73 % 64 78.05 % 1 1.22 % 1 1.22 % 0 0.00 %

Pembs PAR-HERD-S 37 2,406 0.72 % 81 3.37 % 2264 94.10 % 107 4.45 % 59 2.45 % 3 0.12 %

PAR-NEW-IN 3 8 0.00 % 2 25.00 % 4 50.00 % 2 25.00 % 2 25.00 % 0 0.00 %

PAR-OTHER 22 1,884 0.57 % 86 4.56 % 1685 89.44 % 141 7.48 % 112 5.94 % 1 0.05 %

PAR-PERSIS 146 15,340 4.62 % 506 3.30 % 14055 91.62 % 989 6.45 % 771 5.03 % 8 0.05 %








PAR-RAPID 133 366 0.11 % 71 19.40 % 268 73.22 % 21 5.74 % 14 3.83 % 13 3.55 %

SER-FLEXI 50 1,267 0.38 % 93 7.34 % 1123 88.63 % 69 5.45 % 50 3.95 % 1 0.08 %

Powys PAR-HERD-S 28 938 0.28 % 71 7.57 % 825 87.95 % 58 6.18 % 38 4.05 % 4 0.43 %

PAR-NEW-IN 30 981 0.30 % 43 4.38 % 901 91.85 % 44 4.49 % 36 3.67 % 1 0.10 %

PAR-OTHER 2 15 0.00 % 4 26.67 % 9 60.00 % 3 20.00 % 2 13.33 % 0 0.00 %

PAR-PERSIS 49 3,256 0.98 % 111 3.41 % 2755 84.61 % 219 6.73 % 177 5.44 % 213 6.54 %

PAR-RAPID 66 204 0.06 % 27 13.24 % 138 67.65 % 15 7.35 % 14 6.86 % 25 12.25 %

SER-FLEXI 14 388 0.12 % 25 6.44 % 356 91.75 % 10 2.58 % 7 1.80 % 0 0.00 %

S Wales PAR-HERD-S 2 5 0.00 % 0 0.00 % 5 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-OTHER 1 11 0.00 % 0 0.00 % 11 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 3 384 0.12 % 5 1.30 % 371 96.61 % 8 2.08 % 8 2.08 % 0 0.00 %

PAR-RAPID 2 4 0.00 % 0 0.00 % 4 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 3 101 0.03 % 5 4.95 % 92 91.09 % 5 4.95 % 4 3.96 % 0 0.00 %

W Glamorgan PAR-HERD-S 3 118 0.04 % 7 5.93 % 109 92.37 % 5 4.24 % 2 1.69 % 0 0.00 %

PAR-OTHER 1 13 0.00 % 4 30.77 % 9 69.23 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 2 60 0.02 % 1 1.67 % 54 90.00 % 10 16.67 % 5 8.33 % 0 0.00 %

PAR-RAPID 3 6 0.00 % 0 0.00 % 5 83.33 % 1 16.67 % 1 16.67 % 0 0.00 %

Wales - Intermediate TB Carms PAR-HERD-S 3 84 0.03 % 14 16.67 % 67 79.76 % 4 4.76 % 3 3.57 % 0 0.00 %

PAR-NEW-IN 1 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-OTHER 6 351 0.11 % 14 3.99 % 324 92.31 % 16 4.56 % 13 3.70 % 0 0.00 %

PAR-PERSIS 11 903 0.27 % 50 5.54 % 825 91.36 % 46 5.09 % 28 3.10 % 0 0.00 %

PAR-RAPID 15 29 0.01 % 3 10.34 % 24 82.76 % 2 6.90 % 2 6.90 % 0 0.00 %

SER-FLEXI 1 20 0.01 % 12 60.00 % 8 40.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Ceredigion PAR-HERD-S 2 15 0.00 % 0 0.00 % 6 40.00 % 9 60.00 % 9 60.00 % 0 0.00 %

PAR-OTHER 2 148 0.04 % 21 14.19 % 111 75.00 % 17 11.49 % 16 10.81 % 0 0.00 %

PAR-PERSIS 4 550 0.17 % 26 4.73 % 500 90.91 % 28 5.09 % 24 4.36 % 0 0.00 %

PAR-RAPID 5 14 0.00 % 8 57.14 % 6 42.86 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 2 27 0.01 % 2 7.41 % 21 77.78 % 4 14.81 % 4 14.81 % 0 0.00 %








Mid Glamorgan PAR-RAPID 1 6 0.00 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

NE Wales PAR-NEW-IN 93 8,778 2.64 % 265 3.02 % 7933 90.37 % 510 5.81 % 382 4.35 % 198 2.26 %

PAR-OTHER 16 332 0.10 % 21 6.33 % 291 87.65 % 25 7.53 % 20 6.02 % 0 0.00 %

PAR-PERSIS 11 1,619 0.49 % 40 2.47 % 1529 94.44 % 87 5.37 % 59 3.64 % 0 0.00 %

PAR-RAPID 18 183 0.06 % 10 5.46 % 167 91.26 % 6 3.28 % 6 3.28 % 0 0.00 %

SER-FLEXI 44 1,058 0.32 % 76 7.18 % 869 82.14 % 53 5.01 % 43 4.06 % 70 6.62 %

Powys PAR-NEW-IN 13 1,548 0.47 % 37 2.39 % 1432 92.51 % 95 6.14 % 79 5.10 % 0 0.00 %

PAR-PERSIS 2 21 0.01 % 2 9.52 % 17 80.95 % 2 9.52 % 2 9.52 % 0 0.00 %

PAR-RAPID 1 1 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 % 0 0.00 %

S Glamorgan PAR-RAPID 9 42 0.01 % 5 11.90 % 37 88.10 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 4 111 0.03 % 12 10.81 % 98 88.29 % 2 1.80 % 1 0.90 % 0 0.00 %

Shropshire PAR-NEW-IN 1 165 0.05 % 10 6.06 % 144 87.27 % 13 7.88 % 11 6.67 % 0 0.00 %

PAR-PERSIS 1 9 0.00 % 1 11.11 % 6 66.67 % 3 33.33 % 2 22.22 % 0 0.00 %

S Wales PAR-RAPID 2 4 0.00 % 0 0.00 % 3 75.00 % 1 25.00 % 1 25.00 % 0 0.00 %

W Glamorgan PAR-HERD-S 2 11 0.00 % 3 27.27 % 7 63.64 % 1 9.09 % 1 9.09 % 0 0.00 %

PAR-OTHER 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-PERSIS 3 258 0.08 % 17 6.59 % 220 85.27 % 29 11.24 % 21 8.14 % 0 0.00 %

PAR-RAPID 3 4 0.00 % 1 25.00 % 3 75.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Wales - Low TB Gwynedd PAR-NEW-IN 19 865 0.26 % 22 2.54 % 796 92.02 % 69 7.98 % 50 5.78 % 1 0.12 %

PAR-OTHER 1 17 0.01 % 0 0.00 % 17 100.00 % 1 5.88 % 0 0.00 % 0 0.00 %

PAR-RAPID 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %


PAR-OTHER 2 18 0.01 % 1 5.56 % 15 83.33 % 3 16.67 % 2 11.11 % 0 0.00 %

PAR-PERSIS 2 429 0.13 % 7 1.63 % 409 95.34 % 17 3.96 % 13 3.03 % 0 0.00 %

PAR-RAPID 2 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 2 89 0.03 % 4 4.49 % 82 92.13 % 4 4.49 % 3 3.37 % 0 0.00 %

Wales 1395 65499 19.73 % 3198 4.88 % 58755 89.70 % 3932 6.00 % 2984 4.56 % 578 0.88 %

Scotland - Scotland Argyll & Bute PAR-NEW-IN 1 39 0.01 % 1 2.56 % 38 97.44 % 0 0.00 % 0 0.00 % 0 0.00 %








Ayrshire PAR-NEW-IN 3 366 0.11 % 4 1.09 % 345 94.26 % 20 5.46 % 17 4.64 % 0 0.00 %

Dumfries & G PAR-NEW-IN 5 757 0.23 % 10 1.32 % 708 93.53 % 36 4.76 % 29 3.83 % 10 1.32 %

Kirkcudbright PAR-CUL-P 1 352 0.11 % 7 1.99 % 340 96.59 % 8 2.27 % 5 1.42 % 0 0.00 %

PAR-NEW-IN 6 421 0.13 % 55 13.06 % 348 82.66 % 20 4.75 % 18 4.28 % 0 0.00 %

PAR-PERSIS 1 338 0.10 % 2 0.59 % 329 97.34 % 7 2.07 % 7 2.07 % 0 0.00 %

Wigtown PAR-NEW-IN 5 420 0.13 % 11 2.62 % 381 90.71 % 36 8.57 % 28 6.67 % 0 0.00 %

Scotland 22 2693 0.81 % 90 3.34 % 2489 92.42 % 127 4.72 % 104 3.86 % 10 0.37 %

Grand Total Sum: 3471 331966 13314 295738 22682 17635 5314

*For test criteria please refer to Operational notes.




Table 3a. Summary of IFN Gamma results by country and protocol December 2019

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject

n % of total n % n % n % n % n %

England Parallel 141 19591 100.0% 622 3.2% 16824 85.9% 1200 6.1% 986 5.0% 1159 5.9%

Serial/Extended 2 4 0.0% 1 25.0% 2 50.0% 2 50.0% 1 25.0% 0 0.0 %

Total 143 19595 100.0% 623 3.2% 16826 85.9% 1202 6.1% 987 5.0% 1159 5.9%

Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Wales Parallel 84 5393 96.4% 252 4.7% 4926 91.3% 295 5.5% 208 3.9% 7 0.1%

Serial/Extended 7 204 3.6% 29 14.2% 173 84.8% 3 1.5% 2 1.0% 0 0.0 %

Total 91 5597 100.0% 281 5.0% 5099 91.1% 298 5.3% 210 3.8% 7 0.1%

GB Parallel 225 24984 99.2% 874 3.5% 21750 87.1% 1495 6.0% 1194 4.8% 1166 4.7%

Serial/Extended 9 208 0.8% 30 14.4% 175 84.1% 5 2.4% 3 1.4% 0 0.0 %

Total 234 25192 100.0% 904 3.6% 21925 87.0% 1500 6.0% 1197 4.8% 1166 4.6%

143.00

91.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject


England Parallel 2017 261749 99.2% 9916 3.8% 232694 88.9% 18513 7.1% 14477 5.5% 4681 1.8%

Serial/Extended 37 2025 0.8% 110 5.4% 1800 88.9% 110 5.4% 70 3.5% 45 2.2%

Total 2054 263774 100.0% 10026 3.8% 234494 88.9% 18623 7.1% 14547 5.5% 4726 1.8%

Scotland Parallel 22 2693 100.0% 90 3.3% 2489 92.4% 127 4.7% 104 3.9% 10 0.4%

Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Total 22 2693 100.0% 90 3.3% 2489 92.4% 127 4.7% 104 3.9% 10 0.4%

Wales Parallel 1218 60915 93.0% 2842 4.7% 54766 89.9% 3718 6.1% 2818 4.6% 505 0.8%

Serial/Extended 177 4584 7.0% 356 7.8% 3989 87.0% 214 4.7% 166 3.6% 73 1.6%

Total 1395 65499 100.0% 3198 4.9% 58755 89.7% 3932 6.0% 2984 4.6% 578 0.9%

GB Parallel 3257 325357 98.0% 12848 3.9% 289949 89.1% 22358 6.9% 17399 5.3% 5196 1.6%

Serial/Extended 214 6609 2.0% 466 7.1% 5789 87.6% 324 4.9% 236 3.6% 118 1.8%

Total 3471 331966 100.0% 13314 4.0% 295738 89.1% 22682 6.8% 17635 5.3% 5314 1.6%

#######

#######

22.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 9 208 0.8% 30 14.4% 175 84.1% 5 2.4 % 3 1.4 % 0

Parallel - Herd or Group Slaughter 9 851 3.4% 32 3.8% 799 93.9% 33 3.9 % 20 2.4 % 0

Parallel New Infection 77 7512 29.8% 281 3.7% 6248 83.2% 453 6.0 % 354 4.7 % 629 8.4 %

Parallel - new infection cull area 52 9518 37.8% 250 2.6% 8327 87.5% 624 6.6 % 529 5.6 % 412 4.3 %

Parallel - Other 9 578 2.3% 23 4.0% 535 92.6% 26 4.5 % 19 3.3 % 1 0.2 %

Parallel Persistent Infection 47 5998 23.8% 249 4.2% 5380 89.7% 325 5.4 % 245 4.1 % 124 2.1 %

Parallel - persistent infection cull area 6 400 1.6% 15 3.8% 365 91.3% 27 6.8 % 20 5.0 % 0

Parallel - Rapid Testing Two-times IRs 25 127 0.5% 24 18.9% 96 75.6% 7 5.5 % 7 5.5 % 0

Total 234 25192 100.0% 904 3.6% 21925 87.0% 1500 6.0 % 1197 4.8 % 1166 4.6 %

Table 4a. Summaryof IFN Gamma results by submission reason (December 2019)

England - HRA Extended Flexible IFN-GAMMA test 2 4 0.0% 1 25.0% 2 50.0% 2 50.0 % 1 25.0 % 0

Parallel New Infection 1 30 0.1% 8 26.7% 21 70.0% 1 3.3 % 1 3.3 % 0


Parallel - Other 2 11 0.0% 1 9.1% 10 90.9% 0 0 0



England - HRA 71 10077 40.0% 314 3.1% 8757 86.9% 569 5.6 % 476 4.7 % 530 5.3 %

England - Edge Parallel New Infection 62 6601 26.2% 227 3.4% 5431 82.3% 396 6.0 % 314 4.8 % 629 9.5 %

Parallel - new infection cull area 5 2651 10.5% 71 2.7% 2402 90.6% 214 8.1 % 178 6.7 % 0

Parallel - Other 1 111 0.4% 7 6.3% 102 91.9% 3 2.7 % 2 1.8 % 0

Parallel Persistent Infection 1 32 0.1% 3 9.4% 20 62.5% 9 28.1 % 9 28.1 % 0

England - Edge 69 9395 37.3% 308 3.3% 7955 84.7% 622 6.6 % 503 5.4 % 629 6.7 %

England - LRA Parallel New Infection 3 123 0.5% 1 0.8% 114 92.7% 11 8.9 % 8 6.5 % 0

England - LRA 3 123 0.5% 1 0.8% 114 92.7% 11 8.9 % 8 6.5 % 0

Wales - High TB Extended Flexible IFN-GAMMA test 5 140 0.6% 24 17.1% 114 81.4% 3 2.1 % 2 1.4 % 0


Parallel - Other 3 447 1.8% 15 3.4% 416 93.1% 19 4.3 % 15 3.4 % 1 0.2 %



Wales - High TB 67 4684 18.6% 227 4.8% 4277 91.3% 245 5.2 % 173 3.7 % 7 0.1 %

Wales - Intermediate TB Extended Flexible IFN-GAMMA test 2 64 0.3% 5 7.8% 59 92.2% 0 0 0

Parallel - Herd or Group Slaughter 1 9 0.0% 0 0.0 % 8 88.9% 1 11.1 % 1 11.1 % 0


Parallel - Other 3 9 0.0% 0 0.0 % 7 77.8% 4 44.4 % 2 22.2 % 0



Wales - Intermediate TB 20 663 2.6% 44 6.6% 596 89.9% 35 5.3 % 23 3.5 % 0

Wales - Low TB Parallel New Infection 4 250 1.0% 10 4.0% 226 90.4% 18 7.2 % 14 5.6 % 0

Wales - Low TB 4 250 1.0% 10 4.0% 226 90.4% 18 7.2 % 14 5.6 % 0

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 208 6571 2.0% 460 7.0% 5759 87.6% 322 4.9 % 234 3.6 % 118 1.8 %

Parallel - Herd or Group Slaughter 159 8385 2.5% 494 5.9% 7490 89.3% 536 6.4 % 391 4.7 % 11 0.1 %



Parallel - Other 108 5794 1.7% 332 5.7% 4973 85.8% 555 9.6 % 446 7.7 % 43 0.7 %


Parallel - persistent infection cull area 112 21742 6.5% 1038 4.8% 19604 90.2% 1435 6.6 % 1089 5.0 % 11 0.1 %

Parallel - Rapid Testing Two-times IRs 434 1512 0.5% 274 18.1% 1127 74.5% 96 6.3 % 72 4.8 % 39 2.6 %

Serial - Anomalous Reaction 3 27 0.0% 6 22.2% 19 70.4% 2 7.4 % 2 7.4 % 0

Serial - Suspected NSR Herd 3 11 0.0% 0 0.0 % 11 100.0% 0 0 0

Total 3471 331966 100.0% 13314 4.0% 295738 89.1% 22682 6.8 % 17635 5.3 % 5314 1.6 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)

England - HRA Extended Flexible IFN-GAMMA test 29 1937 0.6% 103 5.3% 1722 88.9% 105 5.4 % 67 3.5 % 45 2.3 %

Parallel - Herd or Group Slaughter 4 33 0.0% 0 0.0 % 31 93.9% 3 9.1 % 2 6.1 % 0



Parallel - Other 30 2238 0.7% 93 4.2% 1841 82.3% 316 14.1 % 262 11.7 % 42 1.9 %


Parallel - persistent infection cull area 111 21390 6.4% 1031 4.8% 19264 90.1% 1427 6.7 % 1084 5.1 % 11 0.1 %

Serial - Anomalous Reaction 3 27 0.0% 6 22.2% 19 70.4% 2 7.4 % 2 7.4 % 0

England - Edge Parallel New Infection 950 119706 36.1% 3764 3.1% 107421 89.7% 8235 6.9 % 6452 5.4 % 2087 1.7 %

Parallel - new infection cull area 10 4360 1.3% 114 2.6% 3992 91.6% 308 7.1 % 254 5.8 % 0

Parallel - Other 1 111 0.0% 7 6.3% 102 91.9% 3 2.7 % 2 1.8 % 0


Serial - Suspected NSR Herd 3 11 0.0% 0 0.0 % 11 100.0% 0 0 0

England - LRA Extended Flexible IFN-GAMMA test 2 50 0.0% 1 2.0% 48 96.0% 3 6.0 % 1 2.0 % 0



Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.


England 2054 263774 79.5% 10026 3.8% 234494 88.9% 18623 7.1 % 14547 5.5 % 4726 1.8 %

Wales - High TB Extended Flexible IFN-GAMMA test 124 3279 1.0% 250 7.6% 2911 88.8% 151 4.6 % 115 3.5 % 3 0.1 %

Parallel - Herd or Group Slaughter 146 8218 2.5% 469 5.7% 7365 89.6% 517 6.3 % 374 4.6 % 11 0.1 %


Parallel - Other 48 2577 0.8% 175 6.8% 2270 88.1% 174 6.8 % 131 5.1 % 1 0.0 %


Parallel - Rapid Testing Two-times IRs 376 1224 0.4% 246 20.1% 876 71.6% 87 7.1 % 63 5.1 % 39 3.2 %

Wales - Intermediate TB Extended Flexible IFN-GAMMA test 51 1216 0.4% 102 8.4% 996 81.9% 59 4.9 % 48 3.9 % 70 5.8 %



Parallel - Other 26 833 0.3% 56 6.7% 728 87.4% 58 7.0 % 49 5.9 % 0



Wales - Low TB Extended Flexible IFN-GAMMA test 2 89 0.0% 4 4.5% 82 92.1% 4 4.5 % 3 3.4 % 0


Parallel - Other 3 35 0.0% 1 2.9% 32 91.4% 4 11.4 % 2 5.7 % 0


Parallel - Rapid Testing Two-times IRs 4 5 0.0% 0 0.0 % 5 100.0% 0 0 0

Wales 1395 65499 19.7% 3198 4.9% 58755 89.7% 3932 6.0 % 2984 4.6 % 578 0.9 %

Scotland - Scotland Parallel New Infection 20 2003 0.6% 81 4.0% 1820 90.9% 112 5.6 % 92 4.6 % 10 0.5 %



Scotland 22 2693 0.8% 90 3.3% 2489 92.4% 127 4.7 % 104 3.9 % 10 0.4 %

Year Month Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

WrongEartags

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

2019 January 289 27513 179 1283 5 3 0 0 0 0 86 3 0 3 0 1117 0 63 0 0

February 320 28044 216 1907 2 8 0 0 0 0 166 5 0 9 0 1634 0 74 2 4

March 315 29624 199 1546 1 0 0 0 0 135 0 0 4 0 1316 0 87 0 3

April 269 30320 162 1135 1 8 0 0 0 0 87 3 0 5 0 989 0 40 0 0

May 364 28357 176 1116 2 0 0 0 0 99 0 0 5 0 962 6 42 0 0

June 287 26287 159 964 5 13 0 0 0 0 94 1 0 4 0 773 0 73 0 0

July 316 32549 201 1219 1 13 0 0 0 0 108 3 0 12 0 1020 0 58 0 2

August 262 21887 126 724 1 8 0 0 0 0 84 1 0 6 0 570 0 52 0 0

September 245 25317 134 693 1 7 0 0 0 0 94 0 0 3 1 542 0 45 0 0

October 298 26690 154 743 1 0 0 0 0 58 0 1 4 1 603 25 49 0 0

November 272 30186 169 1080 19 0 0 0 0 72 0 20 0 0 907 60 0 0 0

December 234 25192 138 904 31 504 0 0 0 0 22 0 3 0 0 296 19 0 0 0

Totals (last12 months) 3471 331966 2013 13314 47 587 0 0 0 0 1105 16 24 55 2 10729 110 583 2 9

Table 5a. Summary of PM and culture results for IFNg positive animals.

Country Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

England 2054 263774 1303 10026 442 0 0 0 0 969 9 16 37 1 8434 7 82 0 1

Scotland 22 2693 11 90 0 0 0 0 0 12 0 0 2 0 70 0 5 0 0

Wales 1395 65499 699 3198 145 0 0 0 0 124 7 8 16 1 2225 103 496 2 8

Totals 3471 331966 2013 13314 587 0 0 0 0 1105 16 24 55 2 10729 110 583 2 9

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with anypositive IFNg results.

67

140

76

130

58

131

77

118

119

131

77

110

88

119

108

120

79

137

122

152

90

192

81

147

110

179

104

216

116

199

107

162

188

176

128

159

115

201

136

126

111

134

144

154

103

169

96

138

0

50

100

150

200

250

300

350

400

JanuaryFebruaryM

archAprilM

ayJuneJulyAugustSeptem

berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM

ayJuneJulyAugustSeptem

berO

ctoberN

ovember

Decem

ber

2018

2019

IFNg Positive herds IFNg Negative herds

18401

969

19186

1229

15561

1199

18776

953

19795

947

17980

701

22955

806

18566

682

20202

946

22357

1416

22496

1580

20038

1241

25652

1283

25636

1907

27595

1546

28851

1135

26967

1116

25138

964

30958

1219

20704

724

24198

693

25193

743

28627

1080

23356

904

0

5000

10000

15000

20000

25000

30000

35000

January

February

March

April

May

June

July

August

September

October

Novem

ber

Decem

ber

January

February

March

April

May

June

July

August

September

October

Novem

ber

Decem

ber

2018

2019

IFNg positive animals IFNg non positive animals*

Figure 4b. Number of animals sampled for IFNg and numbers of animals withpositive IFNg results.

*Includes all animals with IFNg test negative, resample and reject outcomes

0

1

2

3

4

5

6

7

8

Janu

ary

Febr

uary

Mar

ch

April

May

June July

Augu

st

Sept

embe

r

Oct

ober

Nov

embe

r

Dec

embe

r

Janu

ary

Febr

uary

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ch

April

May

June July

Augu

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Sept

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r

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ober

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embe

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embe

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2018

2019

%Rejected %Resampled % NEG Fail % POS Fail

% of totalsubmitted

Figure 5. Rates of resamples including the fail rates broken down for positive(POS) and negative (NEG) controls.

0 5 10 15 20 25

WiltshireW Glamorgan

WarksS Wales

Somerset excl NS Glamorgan

PowysPembsOxon

N YorksNorthantsNE Wales

Mid GlamorganLeics & Rut

HerefGwynedd

GwentGr Manchester

GloucsE Sussex

DorsetDevon

DerbyshireCorn & Scilly

CheshireCeredigion

CarmsBucksAvon

%Retested %Resampled

Figure 6. Proportion of samples retested, or requiring a resample, during December 2019 (% submission perCounty)

County %Resampled %Retested

Avon

Bucks

Carms 2.45 % 3.10 %

Ceredigion 1.17 % 2.34 %

Cheshire 6.91 % 8.27 %

Corn & Scilly 3.84 % 4.70 %

Derbyshire 3.59 % 4.51 %

Devon 7.89 % 9.07 %

Dorset 3.64 % 4.30 %

E Sussex 2.14 % 5.00 %

Gloucs 2.94 % 3.98 %

Gr Manchester 8.24 % 9.41 %

Gwent 2.72 % 4.83 %

Gwynedd 6.12 % 10.20 %

Heref 6.52 % 7.77 %

Leics & Rut 1.47 % 2.21 %

Mid Glamorgan

NE Wales 4.08 % 5.99 %

Northants 5.18 % 6.20 %

N Yorks 2.63 % 7.89 %

Oxon 3.74 % 5.47 %

Pembs 3.07 % 4.40 %

Powys 6.16 % 8.05 %

S Glamorgan

Somerset excl N 4.17 % 5.00 %

S Wales

Warks 4.27 % 5.98 %

W Glamorgan 5.66 % 15.09 %

Wiltshire 3.15 % 3.97 %

Country No.animals

No of IFHg+animals

% Positiveanimals No. samples No of IFHg+

samples% Positivesamples

England 18769 623 3.3% 19595 623 3.2%

Wales 5491 281 5.1% 5597 281 5.0%

GB 24260 904 3.7% 25192 904 3.6%

Table 6a (i): Number of animals and submissions tested in December 2019

Country No.animals

No of IFHg+animals



England 230030 10026 4.4% 263774 10026 3.8%

Wales 59269 3198 5.4% 65499 3198 4.9%

Scotland 2015 90 4.5% 2693 90 3.3%

GB 291304 13314 4.6% 331966 13314 4.0%

Table 6b (i): Number of animals and submissions tested in 2019 Up to end of December

Country No.animals

No of IFHg+animals



England 15080 824 5.5% 15274 824 5.4%

Wales 6199 417 6.7% 6280 417 6.6%

GB 21279 1241 5.8% 21554 1241 5.8%

Table 6a (ii): Number of animals and submissions tested in December 2018

Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of December

Country No.animals

No of IFHg+animals



England 165901 9620 5.8% 193638 9620 5.0%

Wales 50414 2858 5.7% 57607 2869 5.0%

Scotland 2080 181 8.7% 2241 181 8.1%

GB 218375 12659 5.8% 253486 12670 5.0%

* Test type NumSubmissions Num Samples Num Positives

Private % Positive

Devon PRV-SP 1 2 0 0

Dorset PRV-SP 2 6 0 0

Worcestershire PRV-SE 1 16 0 0

Total 4 24 0

*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;PRV-SP: Private High Specificity test – England

Table 7a Number of private samples December 2019

Table 7b Number of private samples 2019

* Test type NumSubmissions

NumSamples

NumPositivesPrivate

% Positive

Devon PRV-SE 2 7 0 0

PRV-SP 4 5 0 0

Dorset PRV-SP 4 10 0 0

Gloucestershire PRV-SP 1 4 0 0

Herefordshire PRV-SP 1 2 0 0

Northern Somerset & South Glou PRV-SP 2 2 0 0

Somerset excl North PRV-SE 6 247 16 6.48 %

PRV-SP 7 12 0 0

Staffordshire PRV-SE 1 64 3 4.69 %

Wiltshire PRV-SP 1 3 0 0

Worcestershire PRV-SE 1 16 0 0

SER-OTHER 1 5 0 0

Total 31 377 19 5.04 %

Isle of Man SER-IOM 27 370 2 0.54 %

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 Carmarthenshire 1 22 7 31.8%

Cornwall and Isles ofScilly 1 26 0 0

North East Wales 1 17 2 11.8%

Pembrokeshire 3 74 13 17.6%

Powys 3 60 4 6.7%

TC0077 9 199 26 13.1 %

Table 8a Number of Cattle Antibody Tests December 2019

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 Carmarthenshire 17 597 60 10.1%

Ceredigion 3 372 17 4.6%

Cornwall and Isles ofScilly 2 263 30 11.4%

North East Wales 30 974 119 12.2%

Pembrokeshire 24 841 78 9.3%

Powys 12 440 31 7.0%

South Wales 5 196 16 8.2%

Wiltshire 1 248 9 3.6%

TC0077 94 3,931 360 9.2 %

Table 8b Number of Cattle Antibody Tests 2019

Documents

Monthly Report: Surveillance Project SB4008 IFNg tests for bovine … · 2020-02-05 · Serial test – other reasons NO (APHA Discretion) SER-FLEXI Extended blood test to provide