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Microscopy Terms Magnification – the increase in an object’s apparent size Magnification – the increase in an object’s apparent size Resolution – the power of a microscope to clearly show detail Resolution – the power of a microscope to clearly show detail Clarity Clarity Focus Focus
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ImportanceImportance
One of the most widely used tools in One of the most widely used tools in Biology Biology
Produces enlarged images of objectsProduces enlarged images of objects Microscope animation
Microscopy Terms Microscopy Terms
MagnificationMagnification – the increase in an – the increase in an object’s apparent size object’s apparent size
ResolutionResolution – the power of a – the power of a microscope to clearly show detailmicroscope to clearly show detail ClarityClarity Focus Focus
TYPES OF MICROSCOPESTYPES OF MICROSCOPES
Compound Light Microscope Compound Light Microscope Electron Microscopes Electron Microscopes
Compound Light Microscope Compound Light Microscope Thin sliced and sometimes stained specimen is Thin sliced and sometimes stained specimen is
mounted on a slide to be viewed mounted on a slide to be viewed Uses a light source (a light bulb or mirror)Uses a light source (a light bulb or mirror) Light passes through the specimen and through the Light passes through the specimen and through the
objective lens objective lens total magnification= power of magnification of total magnification= power of magnification of
objective lens x the power of magnification of the objective lens x the power of magnification of the ocular lens ocular lens Ex. 40 X 10 = 400X total magnificationEx. 40 X 10 = 400X total magnification
Limitations of Compound Limitations of Compound Light MicroscopesLight Microscopes
The Resolution decreases as The Resolution decreases as Magnification increases Magnification increases
Electron Microscopes Electron Microscopes Able to view extremely small objects Able to view extremely small objects Uses a Beam of Electrons, rather than Uses a Beam of Electrons, rather than
light to produce an enlarged image light to produce an enlarged image Electron microscopes are more powerful Electron microscopes are more powerful
than LM's than LM's There are several types of electron There are several types of electron
microscopes microscopes
Transmission Electron Microscope Transmission Electron Microscope
(TEM)(TEM) Can magnify objects up to 200,000 X Can magnify objects up to 200,000 X Projects image onto a screen or Projects image onto a screen or
photographic plate photographic plate Greatly magnifies internal details of a Greatly magnifies internal details of a
specimen specimen Cannot be used to view living specimensCannot be used to view living specimens
(TEM)(TEM)
Scanning Electron Microscope Scanning Electron Microscope (SEM)(SEM)
Produces a 3-D image Produces a 3-D image Produce magnified image of Produce magnified image of surfacesurface details details Can magnify up to 100,000 times Can magnify up to 100,000 times Can not be used to view living specimens Can not be used to view living specimens A beam of electrons is passed over the A beam of electrons is passed over the
surfacesurface of a metal coating of a metal coating Showered electrons are projected onto a Showered electrons are projected onto a
fluorescent screen or photographic plate fluorescent screen or photographic plate
(SEM)(SEM)