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Microscopes
Microscope Parts Quiz on Friday!!
Will need to label parts on diagram and complete matching/mult. choice on the function of the parts.
Microscopes
This week’s objectives:
•To name the parts of the microscope and describe their functions
•To describe how to properly use a compound microscope.
•To compare a compound light microscope to a dissecting microscope and electron microscopes.
Label the parts on your microscope picture. Eyepiece
magnification
= 10X
Label the parts on your microscope picture. Eyepiece
magnification = 10X
Arm-Support-proper place to pick up/carry
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage – slide is placed here for viewing
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus. Use ONLY on Scanning power.
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – use for high power focus
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base – support-place other hand here when carrying.
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Has a dimmer switch to adjust the amount of light
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – also adjusts amount of light-works like the pupil of your eye.
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses
Scanning = 4X (shortest)
Low Power = 10X (med. length
High power = 40X (longest)
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses Scanning = 4X (shortest)
Low Power = 10X (med)
High power = 40X (longest)
Revolving nosepiece
Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses Scanning = 4X (shortest)
Low Power = 10X (med)
High power = 40X (longest)
Revolving nosepiece
Tube
Magnification of a Compound Microscope•Because you are looking through multiple lenses the lenses have a “compounding” effect.
•The eyepiece always magnifies 10X
Eyepiece Objective Lens Magnification
Scanning lens (red) 10X
Low Power (yellow)
10X
High Power (blue)
10X
Magnification of a Compound Microscope•Each of the objective lenses have their own magnification
Eyepiece Objective Lens Magnification
Scanning lens (red) 10X
4X
Low Power (yellow)
10X
10X
High Power (blue)
10X 40X
Magnification of a Compound Microscope•You then multiply the eyepiece with the objective lens to determine the total magnification
Eyepiece Objective Lens Magnification
Scanning lens (red) 10X
4X
Low Power (yellow)
10X
10X
100XHigh Power (blue)
10X
40X
400X
40X
General Procedures
•Always carry the microscope with one hand on the Arm and one hand on the Base.
•Wear your glasses, the microscope will focus to your eyesight!
• Keep both eyes open, your brain will learn to ignore the other eye.
• Never use the coarse adjustment after you turn past scanning (4X) power.
Focusing a SpecimenAlways start on scanning power.Bring the stage all the way up and focus the
specimen using the course adjust.Re-center so what you want to focus is at the
pointer!Turn to high power only after re-centeringUse the FINE FOCUS ONLY for low and
highest power. NEVER use the course adjustment after you turn past scanning power.
Making good drawingsMaking good drawings1. Don’t even think of starting your drawing unless you have a
pencil (colored are even better) drawings in pen are unacceptable. This is for two reasons: (a) You can erase pencil! (b) You can shade in areas more easily in pencil
2. Take time to add detail to your drawing. Use your pencil to shade and give your drawing some detail and depth. Use colored pencil when appropriate to add color.
3. In the upper left hand corner of each circle include the specimen name as written on the slide label. In the upper right hand corner, include the magnification (100x or 430x).
4. Label Everything you identify!
100X Cheek cell
nucleus
Cell membrane
Making a Wet Mount SlideMaking a Wet Mount Slide1. Gather a very thin slice/piece of whatever your specimen is. All samples should be paper thickness or thinner!
2. Place ONE drop of water directly over the specimen. The drop should be about ½ the size of a dime.
3. Place the cover slip at a 45 degree angle with one edge touching the water drop, and let the cover slip drop onto the drop of water.
4.Do not push down on the cover slip.
5.Try to avoid bubbles!
Microscope Parts
Label with structure and function
Eyepiec
e
Objective Lens
Magnification
Scanning lens (red)
10X
Low Power (yellow)
10X
High Power (blue)
10X
Magnification of Lenses
Microscopes
