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Marine Pest Monitoring Program Baseline Survey 1 Report

Ichthys Nearshore Environmental Monitoring Program L384-AW-REP-10007

Halleg

Prepared for INPEX

July 2013

Marine Pest Monitoring Program Baseline Survey 1 Report

Ichthys Nearshore Environmental Monitoring Program L384-AW-REP-10007

Marine Pest Monitoring Program Baseline Survey 1 Report Ichthys Nearshore Environmental Monitoring Program

Prepared for INPEX Cardno Page ii

Document Information

Prepared for INPEX

Project Name Ichthys Nearshore Environmental Monitoring Program

File Reference L384-AW-REP-10007_1 Marine Pest Baseline Report.docm

Job Reference L384-AW-REP-10007

Date July 2013

Contact Information

Cardno (NSW/ACT) Pty Ltd Cardno (WA) Pty Ltd Cardno (NT) Pty Ltd

Level 9, The Forum 11 Harvest Terrace Level 6, 93 Mitchell Street

203 Pacific Highway West Perth WA 6005 Darwin NT 0800

St Leonards NSW 2065

Telephone: 02 9496 7700 Telephone: 08 9273 3888 Telephone: 08 8942 8200

Facsimile: 02 9499 3902 Facsimile: 08 9486 8664 Facsimile: 08 8942 8211

International: +61 2 9496 7700 International: +61 8 9273 3888 International: +61 8 8942 8211

www.cardno.com.au www.cardno.com.au www.cardno.com.au

Document Control

Version Date Author Author Initials

Reviewer Reviewer Initials

A 15/11/2012 Emily Jones (Golder Associates) EJ Paul Kennedy (Golder Associates)

Frank Fleer (Golder Associates)

PK

FF

B 21/11/2012 Elena Lazzarotto (Golder Associates)

Peggy O’Donnell

Dan Pygas

EL

POD

DP

Peggy O’Donnell POD

0 27/11/2012 Elena Lazzarotto (Golder Associates)

Peggy O’Donnell

Dan Pygas

EL

POD

DP

Peggy O’Donnell POD

0A 19/06/2013 Peggy O’Donnell POD Joanna Lamb JL

1 05/07/2013 Peggy O’Donnell POD Joanna Lamb JL

This document is produced by Cardno solely for the benefit and use by the client in accordance with the terms of the engagement for the performance of the Services. Cardno does not and shall not assume any responsibility or liability whatsoever to any third party arising out of any use or reliance by any third party on the content of this document.

http://www.cardno.com.au/

http://www.cardno.com.au/


Prepared for INPEX Cardno Page iii

Executive Summary

This report outlines the Baseline Phase of the Marine Pest Monitoring Program undertaken by Golder

Associates Pty Ltd (Golder) and Cardno in August 2012 in accordance with the methods outlined in the

Ichthys Nearshore Environmental Monitoring Plan (NEMP) (Cardno 2012a).

Activities associated with the Ichthys Gas Field Development Project (the Project) have the potential to

introduce marine pests to Darwin Harbour and could encourage the spread of established marine pests that

may be introduced by activities unrelated to the Project. The aim of Baseline survey 1 (B1) was to assess the

presence/absence of pest species at key locations mainly in Darwin Harbour Inner. The observation of a

marine organism that appears to, or could potentially, exhibit pest characteristics in subsequent monitoring

surveys would trigger a response to restrict or prevent the spread of the pest.

The sampling design and species targeted for B1 were developed in consideration of the guidelines provided

by the National System for the Prevention of Marine Pest Incursions, and included inspection of marine

activity hubs prior to commencement of dredging and construction activities associated with the Project.

Survey B1 comprised 25 sampling locations mainly in Darwin Harbour Inner. Locations were chosen to

include:

> Project activities, including dredging, construction, mooring and/or spoil disposal sites;

> Activities unrelated to the Project but which have a relatively high risk of marine pest incursion. For

example, areas that experience a high level of non-Project related vessel movement; and

> Areas in close proximity, but not directly related to, Project activities.

The survey targeted 41 marine species known to display pest characteristics and have the potential to

survive and establish in Darwin Harbour conditions. Characteristics displayed by pest organisms include:

> Occurrence outside their natural range;

> Relatively high local abundances; and

> Traits that enable them to out-compete native species for resources, such as smothering behaviour.

Sample collection was largely carried out as planned by the survey design. Where sampling plan changes

were required during field operations, the detection effort was maintained by using alternative sampling

methods. The only location that could not be surveyed as intended was the Wickham Point LNG (liquified

natural gas) terminal wharf as access was not able to be arranged within the short timeframe allowed for

fieldwork mobilisation.

Two hundred and thirty two specimens were retained for expert examination including barnacles, crabs,

ascidians, bryozoans, molluscs, polychaete worms and fish, as well as dinoflagellate samples. Specimens

were selected for further taxonomic inspection based on similarities with potential pest species. These were

species that can be difficult to confidently identify in the field and require examination of microscopic or

internal features by expert marine taxonomists. The survey team did not record any marine fauna or flora

occurring in excessively high abundance, which is a common feature and possible indication of a pest

species.

Target taxa identified from samples collected during B1 included: Amphibalanus amphitrite (barnacle),

Bugula neritina (bryozoan), and ascidians Botryllus schlosseri, Botrylloides leachi, Didemnum cf. granulatum

and Didemnum sp. The identified taxa are considered to have a relatively low level of biosecurity risk, based

either on their cosmopolitan distribution or the lack of extensive populations currently present at sampling

sites. Golder considers that the sites where targeted ascidians and bryozoans were recorded should remain

in the survey design for continued monitoring of these populations.

Cardno were informed of a pest detection immediately prior to B1 which consisted of three-month old Asian

green mussels (Perna viridis) on the hull of a navy ship which had been berthed at the HMAS Coonawarra

basin after returning from duty in Asia (Australian Defence Force to INPEX, August 2012, pers comm). As

part of B1, two small mussel samples were collected from the seabed within the navy basin HMAS


Prepared for INPEX Cardno Page iv

Coonawarra by epibenthic dredge. Fresh samples were delivered to taxonomists at the Museum and Art

Gallery Northern Territory for rapid examination during fieldwork, but these were comfimed to be native

species. No Asian green mussels were found in any location or by any sampling method during B1.

Five target taxa were collected during B1, including one bryozoan, three ascidians and one barnacle.

The bryzoan B. neritina is one of the most abundant bryozoans in ports and harbours as a member of the

fouling community. The biosecurity threat of this bryozoan is considered to be low given that this species is

relatively well-spread internationally and has been recorded from Darwin Harbour previously without

extensive populations being reported in regular monitoring since 2008 (Maher et al. 2011a, 2011b).

The colonial ascidians B. schlosseri and B. leachi are colonial ascidians that are widespread throughout the

Australian coastline. They can overgrow other fouling organisms and can alter community dynamics on a

range of substrates (NIMPIS 2012b, 2012c). These ascidians were not collected from locations directly

associated with the Project but were collected from sites nearby at Channel Island and East Arm Wharf.

Ascidians in the genus Didemnum were collected from sites nearby at Channel Island and East Arm mooring

and Fort Hill Wharf. This genus can be cryptogenic and one species, Didemnum vexillum, is a well-known

marine pest in the Australasian region (NIMPIS 2012d). None of these ascidian species have been detected

in marine pest monitoring from 2007 or onwards (Cribb et al. 2008; Maher et al. 2001a, b). It is suggested

that a record of the extent of the populations be made if further specimens are observed in subsequent

monitoring surveys to help assess whether this species is displaying pest characteristics in the Darwin

Harbour environment.

The barnacle A. amphitrite has been recorded in Darwin Harbour during regular monitoring since October

2007 from Bayview Marina, Cullen Bay Marina and pontoon, Frances Bay Mooring Basin, Tipperary Waters

Marina, Stokes Hill Wharf and HMAS Coonawarra (Cribb et al. 2008; Maher et al. 2011a, 2011b). It is a

widely distributed species which is often transferred on ship hulls (Stafford and Willan 2007). Given the

already widespread nature of this barnacle, it is considered unlikely that the Project activities would have a

significant effect on the spread of the species throughout Darwin Harbour. It is suggested that this species be

removed from the target species list for the Marine Pest Monitoring Program.

The screening of over 7,000 photoquadrats efficiently increased the geographical range and effort expended

in searching for marine pests. No target species were confirmed, but specimens will be collected of a

potential pest, the green alga Caulerpa racemosa, by divers while monitoring corals in transects at South

Shell Island, Wickham Point and Mandorah at the next opportunity, as identification of this species requires

microscopic examination.


Prepared for INPEX Cardno Page v

Glossary

Term or Acromym Definition

CRIMP Centre for Research on Introduced Marine Pests

CSIRO Commonwealth Scientific and Industrial Research

Organisation

DAFF Department of Agriculture, Fisheries and Forestry

DSDMP Dredging and Spoil Disposal Management Plan –

East Arm

IFFV Illegal foreign fishing vessel

MAGNT Museum and Art Gallery of the Northern Territory

MDAP Monitoring Design Assessment Panel

MITS Marine Invasive Taxonomic Services

National System Australian National System for the Prevention of

Marine Pest Incursions

NEMP Nearshore Environmental Monitoring Plan

NIWA National Institute of Water and Atmospheric

Research

OSM Observation System Method

RBGS Royal Botanic Gardens Sydney


Prepared for INPEX Cardno Page vi

Table of Contents

Executive Summary iii

Glossary v

1 Introduction 1

1.1 Overview 1

1.2 Background 1

1.3 Objectives of the Program 1

1.4 Report Scope 1

2 Methodology 2

2.1 Monitoring Design 2

2.1.1 Monitoring Location 2

2.1.2 Previous Marine Pest Surveys 2

2.1.3 Approach to B1 Survey Design 3

2.2 Locations 4

2.3 Additional Locations 4

2.3.1 Target Species 9

2.4 Observation System Methods 10

2.4.1 Overview 10

2.4.2 Underwater Diver Visual Searches 10

2.4.3 Epifauna Scraping 10

2.4.4 Epibenthic Dredging 10

2.4.5 Benthic Grab Sampling 11

2.4.6 Plankton Netting 11

2.4.7 Visual Shore Searches 11

2.4.8 Dinoflagellate Cores 11

2.4.9 Trapping 11

2.5 Specimen Processing 11

2.6 Identification of Marine Pests 12

2.7 Quality Control of Field Operations 12

2.8 Quality Control of Laboratory Operations 13

2.9 Statistical Methods 13

3 Results 14

3.1 Survey Schedule and Detection Methods 14

3.2 Specimen Collection 16

3.3 Identified Species 17

3.3.1 Overview 17

3.3.2 Target taxa 17

3.3.3 Summary of taxa identified 18

3.4 Results of Additional Data Collection 19

4 Discussion 21

5 Acknowledgements 23

6 References 24


Prepared for INPEX Cardno Page vii

Tables

Table 2-1 B1 survey design, including locations in Darwin Harbour and detection methods 6

Table 2-2 Scope of additional marine pest monitoring undertaken by other NEMP monitoring programs 8

Table 2-3 List of marine pest species targeted during B1 9

Table 2-4 Taxonomic experts enlisted for B1 12

Table 3-1 Survey schedule for B1 14

Table 3-2 Marine specimens collected for taxonomic assessment at locations sampled during B1 16

Table 3-3 Target taxa collected at locations inspected during B1 17

Table 3-4 Summary of inspections of photographs for marine pests 20

Figures

Figure 2-1 Map of survey locations for B1 5

Figure 2-2 Map of sampling or survey locations for marine pests undertaken as part of other NEMP monitoring programs 7

Appendices

Appendix A Location Maps for B1

Appendix B Golder Chain of Custody Form

Appendix C Sample Collection and Specimen Register 2012

Appendix D Record of Additional Data from Photoquadrats


Prepared for INPEX Cardno Page 1

1 Introduction

1.1 Overview

INPEX is the operator of the Ichthys Gas Field Development Project (the Project). The Project comprises the

development of offshore production facilities at the Ichthys Field in the Browse Basin, some 820 km west-south-

west of Darwin, an 889 km long subsea gas export pipeline (GEP) and an onshore processing facility and

product loading jetty at Blaydin Point on Middle Arm Peninsula in Darwin Harbour. To support the nearshore

infrastructure at Blaydin Point, dredging works will be carried out to extend safe shipping access from near East

Arm Wharf to the new product loading facilities at Blaydin Point which will be supported by piles driven into the

sediment. A trench will also be dredged to seat and protect the GEP for the Darwin Harbour portion of its total

length. Dredged material will be disposed at the spoil ground located approximately 12 km north-west of Lee

Point. A detailed description of the dredging and spoil disposal methodology is provided in Section 2 of the

Dredging and Spoil Disposal Management Plan – East Arm (DSDMP) (INPEX 2012).

1.2 Background

In August 2012, Golder Associates Pty Ltd (Golder) and Cardno designed and implemented a Baseline Phase

marine pest survey in Darwin Harbour for the Ichthys Nearshore Environmental Monitoring Plan (NEMP)

(Cardno 2012a). Activities associated with the Project have the potential to introduce marine pests to Darwin

Harbour and could encourage the spread of established marine pests that have been, or could be, introduced

by activities unrelated to the Project. The main vectors of marine pest introduction or spread include:

> Translocation of pest species attached to vessel hulls or in niche areas such as propellers, rudders,

thrusters, stabilisers and other submerged areas of a vessel that provide attachment surfaces and shelter

during vessel movement (Cawthron Institute 2010); and

> Release of adult or larval forms of pest species with ballast water discharge.

The sampling design and species targeted for Baseline survey 1 (B1) were developed in consideration of the

guidelines provided by the Australian National System for the Prevention of Marine Pest Incursions (the

National System) and in line with the Darwin Harbour Marine Pest Monitoring Program that was implemented

by Golder in 2010 (Golder 2010).

1.3 Objectives of the Program

The objectives of the Marine Pest Monitoring Program are:

> Early detection of introduced marine pests into Darwin Harbour; and

> To allow implementation of an introduced marine pest emergency response where an introduced marine

pest is detected.

More specifically, the aim of B1 was to inspect key activity hubs in marine areas around Darwin Harbour to assess

the presence/absence of marine pests prior to the commencement of activities associated with the Project.

1.4 Report Scope

This report has been prepared to describe the B1 marine pest survey carried out in Darwin Harbour in August

2012. The information presented in this report includes:

> The purpose and objectives of B1;

> Brief background information on previous marine pest surveys in Darwin Harbour;

> The approach to B1 survey design;

> Survey locations and methodology;

> Summary of sample collection and specimens identified for specialist taxonomic assessment; and

> Discussion of B1 results.



2 Methodology

2.1 Monitoring Design

2.1.1 Monitoring Location

Darwin Harbour is located in the top end of the Northern Territory (NT). The area experiences a tropical

climate with two distinct seasons. The wet season falls between November and April and is characterised by

thunderstorms, monsoon and cyclone activity. Approximately 64 % of rainfall in Darwin occurs between

January and March (McKinnon et al. 2006). The dry season falls between May and October and is

characterised by warm, dry days and cooler nights.

Darwin Harbour has a catchment area of approximately 2,417 km2 and the surface area ranges between 660

to 1,220 km2 at the lowest and highest astronomical tides, respectively (McKinnon et al. 2006). Darwin

Harbour is considered to be macro-tidal, with an upper tidal range of 7.8 m and mean neap-spring tidal range

of 1.9 to 5.5 m (Williams and Wolanski 2003, McKinnon et al. 2006). This large tidal movement produces

strong currents of over 2 m/s, which can result in very turbid plumes of muddy and sandy substrates that can

extend over wide areas of the harbour (Williams and Wolanski 2003; McKinnon et al. 2006).

2.1.2 Previous Marine Pest Surveys

2.1.2.1 Port Survey for Introduced Marine Species, 1998-99

In recognition of Darwin Harbour as a major commercial shipping hub, a marine pest port survey was carried

out in the Port of Darwin under the National Port Survey Program (the Survey) from 1998 to 1999 (Russell

and Hewitt 2000). The National Port Survey Program employed a nationally adopted set of sampling

protocols developed by the Commonwealth Scientific and Industrial Research Organisation (CSIRO) Centre

for Research on Introduced Marine Pests (CRIMP) and endorsed by the Australian Ballast Water

Management Advisory Committee. The Port of Darwin survey was implemented jointly by marine scientists

from CSIRO CRIMP and the Museum and Art Gallery of the Northern Territory (MAGNT). Specialist

taxonomic identification was primarily undertaken by MAGNT and other institutions including museums and

universities throughout Australia. MAGNT holds the voucher specimens collected during the survey.

The Survey included sampling at 30 sites within the harbour including primary sites at Fort Hill Wharf, Stokes

Hill Wharf, Iron Ore Wharf, Fisherman’s Wharf, East Arm Port, Cullen Bay Marina and Frances Bay Marina.

Sampling was carried out in both the dry season (August 1998) and wet season (March 1999) to account for

seasonal changes that can result in differences in pest populations. The Survey resulted in the identification

of 879 marine species, including new species and others not previously recorded in northern Australia.

The most notable finding of the Survey was the presence of high densities of a new marine pest, the black-

striped mussel (Mytilopsis sallei), in Cullen Bay Marina. This species was subsequently found on the hulls of

small vessels in other Darwin marinas. This was the first record of a mussel species from the Zebra mussel

family (Family: Dreissenidae) in Australia, a native of Central America. This species has the common

opportunist traits of marine pests with very fast growth, early maturity, high fecundity and wide tolerance to

salinity, oxygen and pollution levels (Russell and Hewitt 2000). The response to this incursion, led by the NT

Government and supported by several organisations including MAGNT, Charles Darwin University and

CSIRO, resulted in chemical treatment (sodium hypochlorite and copper sulphate) of the waterways and the

successful eradication of the black-striped mussel from Darwin Harbour (Bax et al. 2002).

As a direct result of the black-striped mussel incursion in previous years, the Aquatic Biosecurity Unit of the

Department of Resources regularly monitors the marine fouling communities of the NT coastline for the early

detection of marine pest populations (Cribb et al. 2010). Routine inspections of vessels wishing to enter

Darwin marinas are also undertaken by the NT Fisheries Group when vessel owners are unable to

demonstrate that the hull has been cleaned or antifouled in Australia. A pest detection immediately prior to

B1 included three month old Asian green mussels (Perna viridis) on the hull of a navy ship which had been

berthed at the HMAS Coonawarra basin after returning from duty in Asia (Australian Defence Force, August

2012 to INPEX, pers. comm.).



2.1.2.2 Targeted Marine Pest Monitoring Program, 2010

As part of the National System, Golder implemented the first targeted marine pest monitoring program in

Darwin Harbour on behalf of the NT Government (Golder 2010). The aim of this program was to detect new

incursions of marine pest species using a survey design developed in accordance with National System

guidelines and tools (GHD 2009) and approved by the national Monitoring Design Assessment Panel

(MDAP). Field surveys were carried out at 22 locations within the harbour using Observation System

Methods (OSMs) designed for detecting a suite of 28 marine pest species. Two surveys were undertaken,

one in April 2010 (post-wet season sampling) and one in August to September 2010 (dry season sampling),

to allow for changes in potential pest populations as a result of seasonal change.

Two potential pest species from the target list were collected during the surveys. These species included a

single Pacific oyster (Crassostrea gigas) shell that was found during a shore search of the Perkins Shipping

Yard during the dry season survey; however, this large valve was likely a discarded shell from oysters

imported and purchased for human consumption and this observation was not considered as a confirmed

indication of this species living in Darwin Harbour. The marine alga Caulerpa racemosa var. lamourouxii was

found during the dry season survey at two sites (Fort Hill Wharf and Weed Reef); however, this variant of the

target species is native and common in tropical and warm temperate seas, and has been recorded from

warmer waters in Australia, including Darwin Harbour. Therefore, this alga was not considered to be of

significant concern with regard to marine pest management (Golder 2010).

The tubeworm Hydroides sanctaecrucis was found to display pest characteristics at Bayview and Cullen Bay

Marinas as it was found in relatively high abundance and appeared to be responsible for eroding the plastic

flotation material on the underside of marina pontoons and piers. This species was not included in the target

list for marine pest species as part of the 2010 monitoring program, although it was recommended that

further consideration be made in regard to the potential risks posed by this prolific fouling population (Golder

2010).

2.1.3 Approach to B1 Survey Design

B1 survey design was developed to align with the National System. The National System has been

developed and accepted as the national approach by Australian governments to reduce the risk posed by

marine pests (DAFF 2012).

This approach involves a national monitoring strategy for standardised and targeted monitoring of marine

species most likely to have significant adverse impacts at locations with the highest risk of marine pest

incursion.

The approach to B1 survey design was to consider the guidance and tools developed under the National

System in order to align the survey as closely as possible with nationally-accepted marine pest surveillance

processes. While the development of the monitoring plan did not utilise the National System’s design tools

nor seek approval from the MDAP, the following considerations were taken into account:

> The Guidelines and Monitoring Manual provided under the National System, including the use of

recommended OSMs for marine pest detection;

> The target pest species, locations and number of samples included in the 2010 Darwin Harbour marine

pest monitoring program, which were selected using the National System tools and approved through the

MDAP;

> The level of detection effort used previously in the 2010 Darwin Harbour marine pest monitoring program;

> Increasing the level of detection for B1 to allow for a greater number of samples and a broader range of

detection methods; and

> Inclusion of key activity hubs associated with the Project.

In addition to the above considerations, target survey locations were selected in order to represent areas that

would inform a comprehensive Baseline Phase Monitoring Program. Locations were chosen to include:

> Project activities, including dredging, construction, mooring and/or spoil disposal activities;

> Activities unrelated to Project activities but that have a relatively high risk of marine pest incursion. For

example, several locations experience high volumes of vessel traffic that could be vectors for marine pest



introduction. Additional locations included those frequently visited by international vessels used for

mooring of seized illegal foreign fishing vessels, or marine facilities with high vessel movement. Many of

these sites correspond to those surveyed in 2010 (refer Golder 2010); and

> Other areas around the harbour in close proximity to Project activities but not directly related to the

Project (e.g. channel markers along the pipeline and shipping route).

Technical refinements were also made to the survey design based on the experience gained by Golder from

the 2010 marine pest monitoring program, including:

> Expansion of the target species list based on finding a potential pest species at some marinas;

> Inclusion of additional tropical pest species based on recommendations from Richard Willan, MAGNT

senior curator for molluscs (pers. comm. R. Willan to E. Lazzarotto, 1 August 2012); and

> Elimination of the use of a beam trawl as epibenthic dredging was less hazardous and more successful

for sample collection in the harbour environment.

2.2 Locations

Survey B1 included 25 locations throughout the wider Darwin Harbour area as shown in Figure 2-1. Detailed

maps of the detection effort at each location are presented in Appendix A.

The locations and detection methods for B1 are presented in Figure 2-1 and Table 3-1. The detection

methods were based on the OSMs presented in the National System monitoring manual (DAFF 2010) and

are further described in Section 2.4. Amendments made to B1 survey design during field operations are

noted and supported by the results presented in Section 3.1.

2.3 Additional Locations

In addition to the monitoring locations and sampling methods described above, surveys being undertaken for

other programs of the NEMP offered the opportunity to collect additional data on marine pest occurrence

throughout Darwin Harbour. Samples and photographs collected as part of other monitoring programs

effectively extended the geographic area within Darwin Harbour that was assessed for marine pest

incursions.

Photographic data collected by divers and remotely using drop cameras as part of the Coral, Seagrass and

Subtidal Benthos Monitoring Programs were screened for the presence of marine pest species. The

identification of benthic fauna collected from subtidal and intertidal areas incorporated searches for marine

pests, and the identification of species caught in crab traps were undertaken as part of the Fish Health

Monitoring Program. Details of the sampling locations, frequency and methods utilised in other monitoring

programs are detailed in separate reports. Sampling or survey locations are presented in Figure 2-2 and a

summary of survey effort is presented in Table 2-2.



Figure 2-1 Map of survey locations for B1



Table 2-1 B1 survey design, including locations in Darwin Harbour and detection methods

Location Number

Survey Location Underwater Diver Visual

Epifauna Scraping

Epibenthic Dredging

Benthic Grab Sampling

Dinoflagellate Coring

Plankton Netting

Trapping Visual Shore Search

1,2 Module offloading facility and associated dredged area

3,4 Product loading jetty and associated dredged area

5 Middle Arm pipeline shore crossing

6,7,8 Cyclone moorings (possibly 3 locations dependent on dredge movements)

9 Dredge spoil disposal site

10,11,12 Channel markers adjacent to the pipeline route – Charlie 1, 2, 7

13,14 Channel markers adjacent to main shipping channel – Bravo 5, 2

15 Weed Reef

16 East Arm Wharf

17 Wickham Point LNG terminal

18 Hudson Creek

19 Channel Island

20 Fort Hill and Stokes Hill wharves

21 HMAS Coonawarra

22 Perkins Shipping

23, 24, 25 Illegal foreign fishing vessel (IFFV) moorings – IFFV 1, 2, 3



Figure 2-2 Map of sampling or survey locations for marine pests undertaken as part of other NEMP monitoring programs



Table 2-2 Scope of additional marine pest monitoring undertaken by other NEMP monitoring programs

Monitoring Program Sample or Survey Type Sample/Survey Locations Baseline Phase Sampling/Survey Effort

Future Sampling/Survey Frequency

Method of Pest Detection

Seagrass Photoquadrats of soft substrata seagrass habitat

Charles Point (2 locations), Woods Inlet, Fannie Bay, East Point, Casuarina Beach, Lee Point

2 surveys x 23 sites x 30 replicates = 1380 photoquadrats examined

Monthly (wet season), every 2 months (dry season) for duration of dredging; every 2 months for a 6 month period after dredging

Inspection of photoquadrats

Coral Photoquadrats of transects through hard substrata, coral habitat

Charles Point, Mandorah, Weed Reef, Channel Island, North East Wickham, South Shell Island

3 surveys x 7 sites x 4 transects x 50 replicates = 4200 photoquadrats examined

Every two months, more frequently if trigger value is exceeded


Subtidal Benthos Infauna samples from soft sediment, subtidal habitats

24 sites within Darwin Harbour, 18 sites, Offshore disposal grounds and 12 sites offshore Control sites

1 survey x 54 sites x 5 replicates =270 samples

1 during dredging, 1 at end of dredging, 1 after 1 year

Identification of infauna specimens

Photoquadrats of biota on soft substrata 24 sites within Darwin Harbour, 18 sites, Offshore disposal grounds and 12 sites offshore Control sites

1 survey x 54 sites x 2 transects x 15 photoquadrats =1620 photoquadrats examined



Intertidal Benthos Infauna samples from soft sediment, intertidal habitats

18 sites within Darwin Harbour (12 Impact and 6 Control sites)

2 surveys x 18 sites x 5 replicates = 180 samples


Identification of specimens

Recreational Fishing and Fish Health

Crab Traps Middle Arm, Elizabeth River, Reicharts Creek, East Arm, Woods Inlet, Racecourse Creek, King Creek, Micketts Creek

6 “boat days”, 20 traps per day = 240 trap samples

During dredging: 36 “boat days”, after, 36 “boat days”

Identification of specimens



2.3.1 Target Species

In the context of marine pest surveillance, the indicator or trigger for action is the detection of a marine

species not detected prior to commencement of Project activities in Darwin Harbour that appears to exhibit,

or could potentially exhibit, pest characteristics.

Pests are typically non-indigenous species. Characteristics displayed by pest organisms include relatively

high local abundances and traits that enable them to out-compete native species for resources (interspecific

competition), such as smothering behaviour.

B1 targeted 41 marine species known to display pest characteristics and with the potential to survive and

establish in Darwin Harbour conditions. This list of target species is presented in Table 2-3 and includes

species from the previous marine pest survey (Golder 2010) and additional considerations as described in

Section 2.3.

Table 2-3 List of marine pest species targeted during B1

Higher Group Species

Algae (red and green algae) Bonnemaisonia hamifera

Caulerpa racemosa

Caulerpa taxifolia

Codium fragile spp. tomentosoides

Grateloupia turuturu

Womersleyella setacea

Ascidiacea (sea squirts) Botrylloides leachi**

Botrylloides aurantius**

Botryllus schlosseri**

Didemnum spp.

Styela plicata**

Symplegma bahraini**

Bryozoa Bugula neritina**

Watersipora subtorquata**

Crustacea (barnacles, crabs) Amphibalanus amphitrite**

Balanus eburneus

Carcinus maenus

Hemigrapsus sanguineus

Hemigrapsus takanoi/pencicillatus

Rhithropanopeus harrisii

Ctenophora (comb jellies) Beroe ovata

Mnemiopsis leidyi

Dinoflagellata (flagellate protists) Alexandrium monilatum

Bivalve Mollusca (oysters, mussels, clams) Crassostrea gigas

Ensis directus

Musculista senhousia

Mya arenaria

Mytilopsis sallei

Perna canaliculus*

Perna viridis



Higher Group Species

Varicorbula gibba

Gastropod Mollusca (snails, whelks) Crepidula fornicata

Rapana venosa

Polychaeta (segmented worms) Hydroides dianthus

Hydroides santaecrucis*

Sabella spallenzanii

Fish Anabas testudineus*

Oreochromis mossambicus*

Siganus luridus

Siganus rivulatus

Tilapia maria*

* Species were added to the list on recommendation from MAGNT

** Species were added based on information presented in Stafford and Willan (2007)

2.4 Observation System Methods

2.4.1 Overview

Marine pest monitoring methods were based on the OSMs described in the DAFF (2010) monitoring manual.

The OSMs are intended as a basic guide to detection methods and have to be adapted to suit the conditions

at survey locations to obtain good quality samples. Changes were made to detection methods based on

recommendations suggested by marine taxonomic experts and from Golder’s previous experience in marine

biosecurity sampling. The eight detection methods used during B1 are summarised in the following sections.

2.4.2 Underwater Diver Visual Searches

Underwater visual surveys were carried out by scuba divers over soft and hard substrates including wharf

pilings, rock revetments and reefs. Each transect covered approximately the area identified in the location

maps (Appendix A). Two divers swam along a pre-determined path; the scientific diver searched for target

species as well as other marine organisms that appeared to be unusual or highly abundant. The support

diver provided assistance to the scientific diver during sample collection and for navigation. Communication

between divers, and between divers and the top-side dive supervisor, was by hard-wired communication

along an umbilical. This allowed the supervisor to direct the divers on the correct heading.

Visual searches were typically conducted for 30-60 minutes but were adjusted relative to the size of the area

under examination. Digital photographs were taken of organisms of interest. Specimens were carefully

scraped from the substrate and retained in plastic bags for sorting and taxonomic identification. The divers

also reported back to the field team on any relevant observations of the underwater environment (i.e. degree

of underwater visibility or the nature of the fouling on submerged structures).

2.4.3 Epifauna Scraping

The field team collected biota from encrusted wharf structures or marker buoys by scraping using a metal or

plastic scraper. An area of approximately 25 cm x 25 cm was collected where possible. Specimens of

particular interest were retained for taxonomic identification.

2.4.4 Epibenthic Dredging

Epibenthic dredging was undertaken to collect fauna inhabiting areas of soft sediment substrata. The

dredges were either 450 mm x 250 mm mouth size with 5 mm mesh net, or 450 mm x 400 mm mouth size

with 10 mm mesh net. These dredges are considered suitable for collection of the target species, which

included mobile crabs and fish, and sessile organisms such as barnacles, algae and bryozoans encrusting

gravelly substrate. The dredge was deployed from the stern of the vessel and lowered until contact was

made with the seafloor. Each tow covered a distance of at least 100 m at an average speed of 0.5 m/s. The



dredge was then retrieved slowly and, once on board, the contents were rinsed out of the dredge net into a

sorting tray and assessed for target species.

2.4.5 Benthic Grab Sampling

Grab samples were collected using a ponar grab with an approximate volume of 8.2 L and 0.05 m2 sample

surface area. The grab was deployed over the side of the vessel with the aid of a winch system. The

contents were sieved (1.0 mm aperture), emptied into a sorting tray and assessed for target species.

2.4.6 Plankton Netting

The water column was sampled for comb jellies by horizontal tows of a hand-deployed plankton net (100 µm

mesh, 700 mm diameter aperture). The net was deployed from the stern of the vessel and towed at a rate

not exceeding 1.0 m/s. The net was towed a distance of approximately 50 m for each sample. At the end of

each tow the net was hauled in and carefully washed with seawater from the outside so the net contents

were washed to the ‘cod-end’ (area of the net where the catch is concentrated). The cod-end was removed

and the sample examined for the presence of target species.

2.4.7 Visual Shore Searches

Field team members searched along specified sections of the coastline for target species or unfamiliar

organisms including crab moults, seaweeds and mollusc shells. As the shoreline could not be readily

accessed at the selected locations owing to access restrictions and vessel size (i.e. vessel too large to get

close to shore in shallow water), searches were carried out with the aid of binoculars. The areas covered by

shore searching are presented in the location maps in Appendix A.

2.4.8 Dinoflagellate Cores

Sediment cores were used to sample dinoflagellates. Samples were collected using either a piston corer

from a vessel or PVC hand corers by divers. Five hand cores or three piston cores were collected at each

sample point so that enough sediment was collected to adequately detect potential cyst species. The piston

corer (100 mm diameter, 500 m length) was deployed over the side of the survey vessel where cores could

not be safely used by divers. The seawater collected by the piston core was removed to leave the top few

centimetres of seawater above the sediment. The remaining seawater and sediment contained in each

piston core were stored in a sampling jar.

Alternatively, PVC cores (30 mm diameter, 250 mm length) were used by divers. These cores were inserted

into the sediment to approximately 150 mm deep. A rubber bung was placed on the top of the core, the core

was removed from the sediment and another bung was inserted into the other end. The cores were returned

to the surface and the top few millimetres of sediment and seawater from each core were siphoned with a

pipette and combined into one composite sample. Dinoflagellate samples were preserved using 1% Lugol’s

solution.

2.4.9 Trapping

‘Opera house’ style traps targeted motile epibenthic species such as crabs. The traps were small, oval,

collapsible traps (640 mm long, 470 mm wide and 200 mm high) with a mesh size of 20 mm and an 80 mm

entry cone. This type of trap was chosen as it has a small mesh size, suitable for retaining targeted species.

Bait bags in the traps were filled with bait and tied securely. Traps were deployed with marker buoys and

tethered to nearby structures. The traps were submerged overnight to allow sufficient time for motile

epifauna to detect the bait and enter the trap. On retrieval of the trap, specimens of particular interest were

retained for taxonomic identification.

2.5 Specimen Processing

Laboratory facilities were provided by NT Fisheries. Samples were examined, sorted and packaged for

transfer to taxonomic specialists by field scientists during field operations. Specimen preservation was

undertaken according to guidelines in the National System monitoring manual (DAFF 2010) and from

recommendations provided by taxonomic experts enlisted for the Nearshore Environmental Program.

Typically, specimens were fixed in 10% seawater buffered formalin then transferred to 70 % ethanol after 24

hours fixing.



Samples were labelled with a unique identifier to align with the National System (DAFF 2010), which

included identifiers for the survey (three characters for the port and four numbers for the date), the site (four

characters), the method (two characters) and a specimen number (two numbers). For example, for a

specimen collected by epibenthic dredge at Perkins Shipping in Darwin Harbour during August 2012, the

label would be DRW1208PKSHED01. Specimens were also labelled with a taxon code (i.e. CR for

crustacean, PC for polychaete) to assist when packaging samples for the various specialists.

2.6 Identification of Marine Pests

Prior to commencing fieldwork, taxonomic specialists were sought with the assistance of MAGNT

representative, Dr Richard Willan. Experts were then enlisted from several organisations in Australia and

New Zealand, including the Museum and Art Gallery of the Northern Territory (MAGNT), the Royal Botanic

Gardens Sydney (RBGS) and the Marine Invasive Taxonomic Services unit (MITS) of New Zealand’s

National Institute of Water and Atmospheric Research (NIWA). Table 2-4 outlines the taxa requiring

identification and the respective taxonomic expertise enlisted to assist in identification of specimens collected

during the survey.

Table 2-4 Taxonomic experts enlisted for B1

Taxon Name Organisation Position

Algae Professor Alan Millar RBGS Principal Research Scientist

Ascidiacea Mike Page MITS Marine Ecologist

Crustacea Dr Shane Ahyong MITS Marine Biodiversity and Biosecurity (manager)

Fish Dr Michael Hammer MAGNT Curator, Fishes

Mollusca Dr Richard Willan MAGNT Senior Curator, Molluscs

Editor, Academic Publications

Polychaeta Dr Chris Glasby MAGNT Marine Curator

Dinoflagellates Dr Gustaaf Hallegraeff University of Tasmania Professor

On completion of the survey, samples were packaged using chain of custody procedures and the mollusc,

fish and polychaete worm specimens were transferred to MAGNT taxonomic experts by the Golder field

team in Darwin. Cardno staff with expertise in the identification of marine invertebrates (including pest

species) undertook a preliminary screening of barnacle, bryozoan and crab specimens. Cardno taxonomic

specialists included:

> Rick Johnson, formerly of the Marine Invertebrate Department at The Australian Museum, who inspected

barnacles and crab specimens; and

> Dr Rad Nair who has a doctorate in bryozoan taxonomy and extensive experience in this field working for

universities in India and Japan, and as a research associate for the University of New South Wales, the

University of Wollongong, the Australian Museum and CSIRO.

Following screening by Cardno taxonomic specialists, uncommon and difficult suspected marine pests

specimens were sent to the relevant experts identified in Table 2-4 for final verification.

2.7 Quality Control of Field Operations

Cardno and subcontractor personnel involved in the collection of marine pest data underwent training prior to

field work being undertaken. This included team members who were already familiar with the identification of

target species. This training involved the following approach:

> Overview of field survey methodology;

> Desktop learning using detailed information and images of target species;

> Presentation and field guide information on target species (including field instruction) delivered by

Golder’s team leaders; and



> Onsite training of Cardno and subcontracted dive team members, supervised by Golder’s team leaders.

The dive team involved in marine pest collection were therefore familiar with visual search techniques and

the identification of species. Key morphological characteristics of target species were highlighted (e.g. body

shape, behaviours, colour patterns). Divers were instructed to collect unfamiliar species and species

displaying invasive characteristics.

2.8 Quality Control of Laboratory Operations

Sample collection and specimens retained for taxonomic assessment were registered on a Sample and

Specimen Register.

Chain of custody forms were used when transferring samples from the field base to the taxonomists. An

example of Golder’s standard chain of custody form is attached in Appendix B. A copy was retained by the

field team as a record. The original form was sent with the specimens, and personnel receiving the package

were to verify that specimens had been accounted for. The chain of custody form contained the following

information:

> List of sample codes;

> Date relinquished;

> Name of the person(s) who relinquished the samples; and

> Name and address of person or organisation designated to receive the samples.

2.9 Statistical Methods

The basis of the data analysis for marine pest monitoring is the assessment of target species presence or

absence. Statistical data analysis is not required to meet the objectives of Marine Pest Monitoring Program.



3 Results

3.1 Survey Schedule and Detection Methods

The schedule of dates, locations and detection methods used during B1 (August 2012) are presented in

Table 3-1.

As the schedule deviated from the proposed plan at some locations, the details of these amendments are

described below in Table 3-1 with observations made by the field team. The ‘Sample Collection and

Specimen Register’ included as Appendix C includes coordinates for sample collection during B1.

Table 3-1 Survey schedule for B1

Location number

Survey Location Date Detection Method

1 Module offloading facility 1 August Dinoflagellate coring (piston corer)

4-6 August Benthic grab sampling, epibenthic dredging, plankton netting

12 August Visual shore search

2 Dredged area associated with the module offloading facility

1 August Dinoflagellate coring (piston corer)

4-5 August Benthic grab sampling, epibenthic dredging, plankton netting

3 Product loading jetty 4-5 August Benthic grab sampling, epibenthic dredging, plankton netting


4 Dredged area associated with product loading jetty

4-5 August Benthic grab sampling, epibenthic dredging, plankton netting, visual shore search

5 Middle Arm pipeline shore crossing 4-5 August Benthic grab sampling, epibenthic dredging, plankton netting

6 Proposed cyclone mooring (Middle Arm)

11 August Epibenthic dredging

7 Temporary cyclone mooring (East Arm) 7 August Benthic grab sampling, epibenthic dredging

8 Additional proposed cyclone mooring No location designated in 2012

9 Dredge spoil disposal site 5 August Benthic grab sampling, epibenthic dredging, plankton netting

10 Charlie 1 channel marker 6 August Benthic grab sampling, epibenthic dredging

13 August Epifauna scraping





13 Bravo 5 channel marker 5-6 August Benthic grab sampling, epibenthic dredging

14 Bravo 2 channel marker 5 August Benthic grab sampling, epibenthic dredging

15 Weed Reef 6 August Plankton netting

13 August Underwater diver visual search



Location number

Survey Location Date Detection Method

16 East Arm Wharf 7 August Benthic grab sampling, epibenthic dredging, epifauna scraping, plankton netting

10 August Underwater diver visual search


17 Wickham Point LNG terminal No access to location therefore not sampled in 2012

18 Hudson Creek 3 August Dinoflagellate coring (piston corer)

4-6 August Benthic grab sampling, dinoflagellate coring (piston corer), epibenthic dredging, plankton netting

7 August Epifauna scraping, visual shore search


19 Channel Island 5 August Benthic grab sampling, epibenthic dredging, plankton netting

11 August Underwater diver visual search, dinoflagellate coring (diver hand core)

20 Fort Hill and Stokes Hill wharves 7 August Benthic grab sampling, epifauna scraping, epibenthic dredging, plankton netting, trapping

12-13 August

Visual shore search, underwater diver visual search, dinoflagellate coring (diver hand core)

21 HMAS Coonawarra 11 August Benthic grab sampling, epibenthic dredging, plankton netting

22 Perkins Shipping 11 August Epibenthic dredging, plankton netting

23 IFFV1 6 August Benthic grab sampling, epifauna scraping, epibenthic dredging, plankton netting

24 IFFV2 7 August Epibenthic dredging, plankton netting

25 IFFV3 7 August Epibenthic dredging, plankton netting

The changes to the survey design are summarised below, including the reasons for these changes and

significant field observations:

> Locations 1 and 2 – Sampling was carried out further offshore than planned for some grab samples and

epibenthic dredging because shallow water depths close to shore prohibited survey vessels from

accessing the pre-determined sampling points;

> Locations 1 to 4 – Trapping could not be carried out at these sites owing to the spring tide conditions

whereby strong currents were likely to have washed away the traps from the area. Trapping should be

included in the sampling regime for these locations once the marine structures have been constructed,

such that crab traps could be tied securely to the structures. In addition, crabs were sampled in epibenthic

dredges deployed at these locations;

> Location 5 – Dinoflagellate cores were not collected from this location as the piston corer was ineffective

at retrieving sediment samples from the more sandy substrata in parts of Darwin Harbour;

> Locations 10 to 14 – Diving was not possible on the marker buoys at these locations during the spring

tides, although epifauna was able to be scraped from the waterline of marker buoys as an alternative

means of sample collection. It was noted that the position of marker buoys do not correspond directly to

the channel marker locations on the navigational chart as these markers represent areas along the

shipping route rather than specific marker buoys; however, some marker buoys were positioned within

the zone of these channel marker locations;

> Location 17 – Sampling at Wickham Point was unable to proceed as access to the site was not granted

during B1;



> Location 18 – Epifauna scraping was included in the sample collection at Hudson Creek along the

concrete wall of the ship yard;

> Location 20 – The underwater diver visual search on the southern/seaward side of Stokes Hill Wharf was

relocated to the northern/landward side of Fort Hill Wharf. Neptune Marine Services, the commercial dive

company providing assistance during this survey, noted that there was higher vessel traffic on this section

of Fort Hill Wharf, whilst the outside of Stokes Hill Wharf was primarily used for recreational fishing with

infrequent vessel visits to this area. The area enclosed by wharf structures on the western end of Fort Hill

Wharf could not be accessed with the vessels used during this survey; therefore, sampling effort was re-

positioned within this location to maintain detection effort;

> Locations 21 and 22 – Plankton netting was included as a sampling method at HMAS Coonawarra,

increasing the detection effort for comb jellies; and

> Location 23 – Epifauna scraping was included at IFFV1 as the marker buoy was heavily fouled.

3.2 Specimen Collection

Table 3-2 presents a summary of the specimens collected from each location. Two hundred and twenty four

specimens were identified for further examination, including barnacles, crabs, ascidians, bryozoans,

molluscs, polychaetes and fish, as well as dinoflagellate core samples. The specimens were selected for

further taxonomic inspection based on similarities with potential pest species, but were also typically species

that can be very difficult to confidently identify in the field as examination of microscopic or internal features

was required. For instance, barnacle identification often relies on inspection of the internal animal.

The Sample Collection and Specimen Register is included as Appendix C, with details on all samples taken

and specimens retained during B1 undertaken in August 2012.

Table 3-2 Marine specimens collected for taxonomic assessment at locations sampled during B1

Location Number

Survey Location Taxon (no. of specimens) Total Number of Specimens

Per Site

1 Module offloading facility Bivalvia (1), Crustacea (5), Dinoflagellata (1) 7

2 Dredged area associated with the module offloading facility

Ascidiacea (2), Bryozoa (1), Crustacea (2), Dinoflagellata (1), Polychaeta (1)

7

3 Product loading jetty No specimens retained 0

4 Dredged area associated with product loading jetty

Ascidiacea (2), Bivalvia (2), Crustacea (1), Fish (1)

6

5 Middle Arm pipeline shore crossing Bivalvia (1), Crustacea (2) 3

6 Proposed cyclone mooring (Middle Arm)

Algae (1), Bivalvia (1), Crustacea (4) 6

7 Temporary cyclone mooring (East Arm) Ascidiacea (8), Crustacea (2) 10

9 Dredge spoil disposal site Bivalvia (1), Crustacea (2) 3

10 Charlie 1 channel marker Bivalvia (5), Crustacea (12) 17



13 Bravo 5 channel marker Bivalvia (1), Crustacea (4) 5

14 Bravo 2 channel marker Ascidiacea (1), Bivalvia (2), Bryozoa (1), Crustacea (2), Polychaeta (5)

11

15 Weed Reef Algae (1), Bivalvia (2), Polychaeta (1) 4

16 East Arm Wharf Algae (2), Ascidiacea (5), Bivalvia (8), Crustacea (13), Dinoflagellata (1), Polychaeta (2)

31

18 Hudson Creek Bivalvia (3), Crustacea (1), Dinoflagellata (3), Gastropoda (1)

8



Location Number

Survey Location Taxon (no. of specimens) Total Number of Specimens

Per Site

19 Channel Island Algae (1), Ascidiacea (2), Bivalvia (1), Crustacea (2), Gastropoda (3)

9

20 Fort Hill and Stokes Hill wharves Algae (5), Ascidiacea (2), Bivalvia (6), Bryozoa (1), Crustacea (4), Dinoflagellata (3), Polychaeta (1)

22

21 HMAS Coonawarra Algae (2), Ascidiacea (2), Bivalvia (12), Crustacea (3), Fish (3), Polychaeta (1)

23

22 Perkins Shipping Bivalvia (1), Crustacea (5), Polychaeta (1) 7

23 IFFV1 Ascidiacea (1), Bivalvia (2), Crustacea (10) 13

24 IFFV2 Ascidiacea (1), Crustacea (2), Polychaeta (1) 4

25 IFFV3 Bivalvia (2), Crustacea (7) 9

3.3 Identified Species

3.3.1 Overview

The Sample Collection and Specimen Register (Appendix C) includes details on samples taken and

specimens retained during B1 undertaken in August 2012.

Two hundred and thirty two samples were collected for further examination, including barnacles, crabs,

ascidians, bryozoans, molluscs, polychaetes and fish, as well as dinoflagellate core samples. The specimens

were selected for further taxonomic inspection based on similarities with potential pest species, but were also

typically species that can be difficult to confidently identify in the field as examination of microscopic or

internal features was required. For instance, barnacle identification often relies on inspection of the internal

animal.

3.3.2 Target taxa

Target taxa identified from samples collected during B1 included:

> Bugula neritina – bryozoan;

> Botryllus schlosseri – ascidian;

> Botrylloides leachi – ascidian;

> Didemnum cf. granulatum – ascidian;

> Didemnum sp. – ascidian; and

> Amphibalanus amphitrite – barnacle.

Table 3-3 summarises the locations from which these taxa were collected.

Table 3-3 Target taxa collected at locations inspected during B1

Taxa Location Sample collection method

Bugula neritina Bravo 2 Epibenthic dredge

Fort Hill Wharf Diver visual

Botryllus schlosseri Channel Island Epibenthic dredge

Botrylloides leachi East Arm Wharf Epifauna scraping

Didemnum cf. granulatum Fort Hill Wharf Diver visual

Didemnum sp. Channel Island Epibenthic dredge

East Arm mooring Epibenthic dredge

Amphibalanus amphitrite Bravo 5 Epibenthic dredge, benthic grab



Taxa Location Sample collection method

Charlie 1 Epibenthic dredge, benthic grab,

epifauna scraping

Charlie 2 Epibenthic dredge

Charlie 7 Epibenthic dredge, benthic grab

Cyclone mooring Epibenthic dredge

East Arm mooring Epibenthic dredge

East Arm Wharf Epibenthic dredge, benthic grab,

epifauna scraping, diver visual

Hudson Creek Epibenthic dredge

HMAS Coonawarra Epibenthic dredge

IFFV1 Epibenthic dredge, benthic grab,

epifauna scraping

IFFV2 Epibenthic dredge

IFFV3 Epibenthic dredge, benthic grab

Module Offloading Facility – Site 1 Epibenthic dredge

Module Offloading Facility – Site 2 Epibenthic dredge

Perkins Shipping Epibenthic dredge

Product Loading Facility – Site 3 Epibenthic dredge

3.3.3 Summary of taxa identified

3.3.3.1 Algae

Twenty-two algal specimens were examined, with nineteen specimens identified to species level and three

specimens noted as animals (likely hydroids). Thirteen algal species were recorded from the B1 specimens.

This included Caulerpa racemosa var. lamourouxii, is a native variant of the target species Caulerpa racemosa.

3.3.3.2 Ascidiacea

Eighteen species were identified from 27 specimens. These identifications included B. leachi, B. schlosseri,

and Didemnum spp. (Didemnum cf. granulatum and Didemnum sp.) which were included in the B1 target

species list. Four remaining specimens included three sponges and an indeterminate ascidian specimen

(and empty transparent test).

3.3.3.3 Bryozoa

Two bryozoan specimens were identified by Dr Denis Gordan, but were not species on the target list. Dr Rad

Nair identified five bryozoan species from eight specimens, which included the target species Bugula

neritina.

3.3.3.4 Crustacea

Eighty-three barnacle samples, most containing multiple specimens, were initially screened in the Cardno

laboratory, with four questionable samples sent on to MITS for a second opinion on the identification. Cardno

provisionally identified the following taxa:

> Tetraclita/Elminius sp;

> Megabalanus tintinnabulum;

> M. rosa;

> Amphibalanus reticulatus; and

> A. amphitrite.



The M. rosa samples sent for secondary examination were identified as Striatobalanus amaryllis which is not

a target species. This group of ‘pink’ barnacles display similar characteristics that are regarded as very

difficult to identify and are the subject of taxonomic revision (Wells et al. 2009).

A. amphitrite specimens sent for secondary examination included the taxa Amphibalanus reticulatus and

Solidobalanus sp. (but could not be identified further as the shells were empty). A. reticulatus and

Solidobalanus sp. are not target species or genera; however, A. amphitrite is listed as a target species.

Native crabs were also identified, including Xanthidae (six specimens), Portunidae (one specimen),

Porcellenidae (five specimens), Ocypodidae (two specimens) and Grapsidae (eight specimens). No species

from the target list were identified.

3.3.3.5 Dinoflagellate samples

Five dinoflagellates were identified but none were the target species. In addition, 15 other taxa were

identified including diatoms.

3.3.3.6 Fish

Four fish specimens were identified but none of these were from the target list.

3.3.3.7 Mollusca

Ninety mollusc specimens were examined by MAGNT, with 42 species identified. Species identified were

native to the NT. The highest proportion of these specimens was bivalves with some gastropods (snails).

One specimen was discarded as an unidentifiable individual.

3.3.3.8 Polychaeta

Twenty six polychaete specimens were assessed. Ten native species were identified (from 12 specimens),

while ten specimens were too poorly preserved, had no head or were empty tubes that could not be

identified further than family level. One specimen was a peanut worm (Sipuncula) and three specimens were

mangrove ‘worms’ that were actually molluscs.

3.4 Results of Additional Data Collection

The results of the inspection of photographs from Seagrass, Coral, Subtidal Benthos (Epifauna) and

Research Angling monitoring programs are presented in Appendix D and summarised in Table 3-4.

No marine pests were verified on the basis of inspection of photographs. The provisional identification of the

target species of green alga C. racemosa could not be confirmed because microscopic inspection of this

species is necessary to distinguish it from the native variant Caulerpa racemosa var. lamourouxii, and divers

could not safely dive in locations where it was identified in photographs. Collections of this alga from coral

transects will be attempted at the next, earliest opportunity and reported on following the first during-dredging

marine pest survey.



Table 3-4 Summary of inspections of photographs for marine pests

No pest crab species were caught at the Research Angling locations Bynoe Harbour and Adelaide River.

* Indicates an outcome requiring further verification. Divers for the Coral Monitoring Program were provided with the location of potential

pests prior to the October 2012 routine survey and requested to collect physical specimens for further verification. Divers could not

access South Shell Island and Wickham Point could due to dredge activities. Mandorah is a contingency coral monitoring site and

therefore was not scheduled to be visited as part of the bi-monthly routine monitoring done in October 2012; it was not surveyed for

pests in October due to time constraints.

Scope/Marine Pest No. of Potential Identifications Outcome

Location Mandorah Channel Island

South Shell Island

Northeast Wickham

Point

Weed Reef

Charles Point

Coral

C. racemosa 5 0 27 4 0 0 *

Hydroides dianthus/ sanctaecrucis

3 6 1 5 16 2 None verified as pest species

Sabella spallanzanii 0 0 5 5 0 2

None verified as pest species

Seagrass 0 0 0 0 0 0 No action required

Subtidal Benthos 0 0 0 0 0 0 No action required

Research Angling (Crabs)

No action required

Carcinus maenus 0 0 0 0 0 0 No action required

Hemigrapsus sanguineus

0 0 0 0 0 0 No action required

Hemigrapsus takanoi/pencicillatus


Rhithropanopeus harrisii




4 Discussion

B1 was carried out in August 2012 and inspected 25 locations throughout Darwin Harbour. Two hundred and

thirty two samples were retained for examination by specialist taxonomists. Samples were retained for further

examination where a specimen was sufficiently similar to a target pest species or when examination of

microscopic or internal features was required to confidently identify the specimen. The specimens retained

included barnacles, crabs, ascidians, bryozoans, molluscs, polychaetes and fish, as well as dinoflagellate

core samples. Five target taxa were collected during B1, including one bryozoan, three ascidians and one

barnacle.

B. neritina is one of the most abundant bryozoans in ports and harbours as a member of the fouling

community (NIMPIS 2012a). It grows well on pier piles, vessel hulls, buoys and similar submerged surfaces

and in ships’ intake pipes and condenser chambers. The Aquatic Biosecurity unit of NT Fisheries regularly

monitors biofouling communities in Darwin Harbour and its marinas using settlement collectors. They

reported B. neritina from Frances Bay Mooring Basin, and Cullen Bay and Bayview Marinas in October 2007

(Cribb et al. 2008). The biosecurity threat of this bryozoan is considered to be low given that this species is

relatively well-spread internationally and has been recorded from Darwin Harbour previously without

extensive populations being reported in regular monitoring since 2008 (Maher et al. 2011a, b).

B. schlosseri and B. leachi are colonial ascidians that are widespread throughout Australia’s coastline. They

can overgrow other fouling organisms and can alter community dynamics on a range of substrates (NIMPIS

2012b, 2012c). These ascidians were not collected from locations directly associated with the Project but

were collected from sites nearby at Channel Island and East Arm Wharf.

The other target ascidians recorded during B1 were Didemnum spp. This genus can be cryptogenic and it is

Didemnum vexillum that is a well-known marine pest in the Australasian region (NIMPIS 2012d). D. vexillum

tends to be found as a thin rope-like form or as a thick encrusting mat in areas with rough conditions

(NIMPIS 2012d). The Didemnum spp specimens were not collected from locations directly associated with

the Project but were collected from sites nearby at Channel Island and East Arm mooring and Fort Hill

Wharf. None of these ascidian species have been detected in marine pest monitoring from 2007 or onwards

(Cribb et al. 2008; Maher et al. 2001a, b). Golder suggests that a record of the extent of the populations be

made if further specimens are observed in subsequent monitoring surveys to help assess whether this

species is displaying pest characteristics in the Darwin Harbour environment.

The barnacle A. amphitrite has been recorded in Darwin Harbour during regular monitoring since October

2007 from Bayview Marina, Cullen Bay Marina and pontoon, Frances Bay Mooring Basin, Tipperary Waters

Marina, Stokes Hill Wharf and HMAS Coonawarra (Cribb et al. 2008; Maher et al. 2011a, b). It is a species

which is widely distributed as it is often transferred on ship hulls (Stafford and Willan 2007). Given the

already widespread nature of this barnacle, it is considered unlikely that the Project activities would have a

significant effect on the spread of the species throughout Darwin Harbour.

Mussels were found on the hull of a vessel berthed at HMAS Coonawarra (Australian Defence Force, August

2012 to INPEX, August 2012, pers. comm.) and verified as Asian Green mussel (Perna viridis) by Dr Richard

Willan of MAGNT. The mussel was collected in the days prior to the scheduled inspection of the navy basin

that was undertaken as part of B1. No Asian green mussels were found at HMAS Coonawarra or at other

sites in Darwin Harbour during B1.

A number of target species were recorded during B1, including a barnacle, ascidians and a bryozoan. These

taxa are currently considered to have a relatively low level of biosecurity risk, based either on their

cosmopolitan distribution or the lack of extensive populations currently present at Darwin Harbour sites.

Golder suggests that a record of the extent of the populations be made if further specimens are observed in

subsequent monitoring surveys to help assess whether this species is displaying pest characteristics in the

Darwin Harbour environment.

In regard to A. amphitrite, it is suggested that this species be removed from the target species list for the

Marine Pest Monitoring Program. This species is known to be widely distributed around Darwin Harbour and

it is considered unlikely that the Project activities would have a significant effect on the spread of the species.



In regard to the long-term Marine Pest Monitoring Program, it is suggested that the number of sampling

locations and the target species list could be reduced so as to focus effort at a scale appropriate to the

Project activities. Consideration could be given to removing locations which are considered to pose a low-

biosecurity risk in terms of Project activities or non-Project related vessel movement in Darwin Harbour.



5 Acknowledgements

This final report was prepared by Emily Jones (Golder) and Elena Lazzarotto (Golder). As part of the Golder

report review process, technical review was provided by Paul Kennedy (Golder) and senior review provided

by Frank Fleer (Golder) and Cary Ehrman (Golder). Dan Pygas and Dr Peggy O’Donnell undertook the

Cardno review and revision of the draft report from Golder.

The field team who contributed to sampling and sample sorting included: Elena Lazzarotto and Emily Jones

as Golder’s field team leaders, Dan Pygas as Cardno’s team representative and scientific diver, Golder’s

field team members Adam Cohen, Julia Wallis, Ryan Baring and Katy Griffiths, and scientific divers Dr Simon

Bryars and James Brook. The field team appreciated the assistance provided by the variety of survey vessel

crews, the diving assistance from Neptune Marine Services, Darwin Port Corporation and the laboratory

space provided by NT Fisheries. Rick Johnson and Dr Rad Nair undertook preliminary screening

identifications of crustacean and bryozoan samples prior to dispatch to taxonomic specialists from MAGNT

(Dr Richard Willan, Dr Michael Hammer, Dr Chris Glasby), the Royal Botanic Gardens Sydney (Prof. Alan

Millar), NIWA (Dr Shayne Ahyong, Mike Page) and the University of Tasmania (Gustaaf Hallegraeff).



6 References

Bax, N., Hayes, K., Marshall, A., Parry, D. and Thresher, R. (2002). Man-made marinas as sheltered islands for alien marine organisms: Establishment and eradication of an alien invasive marine species. In: Turning the tide: the eradication of invasive species. Veitch CR, Clout MN (eds.). IUCN SSC Invasive Species Specialist Group. IUNC, Gland, Switzerland and Cambridge, UK.

Cardno (2012a). Ichthys Project: Nearshore Environmental Monitoring Plan (NEMP). Prepared for INPEX, August 2012.

Cardno (2012b). Coral Monitoring Program Baseline Report. Ichthys Nearshore Environmental Monitoring Program. L384-AW-REP-10003. Rev 0. Prepared for INPEX November 2012.

Cardno (2012c). Seagrass Monitoring Program Baseline Report. Ichthys Nearshore Environmental Monitoring Program. L384-AW-REP-10002. Rev 0. Prepared for INPEX October 2012.

Cardno (2012d). Subtidal Benthos Monitoring Program Baseline Report. Ichthys Nearshore Environmental Monitoring Program. L384-AW-REP-10005. Rev 0. Prepared for INPEX November 2012.

Cardno (2012e). Recreational Fishing Monitoring Program Baseline Report - Season 1. Ichthys Nearshore Environmental Monitoring Program. L384-AW-REP-10008. Rev 0. Prepared for INPEX November 2012.

Cawthron Institute (2010). Vessel biofouling as a vector for the introduction of non-indigenous marine species to New Zealand: Slow-moving barges and oil platforms. Biosecurity New Zealand Project ZBS2005-02. For Policy and Risk Directorate, Biosecurity New Zealand. 40 pp.

Cribb, H., Maher, J. and Beatty, A. (2008). Monitoring for Marine Pests - Darwin Harbour: 2007-08 Report. Northern Territory Government, Department of Resources. 32 pp.

Cribb, H., Maher, J. and Beatty A. (2010). Monitoring for Marine Pests - Darwin Harbour: 2008-09 Report. Northern Territory Government, Department of Resources. 25 pp.

Department of Agriculture, Fisheries and Forestry (DAFF) (2010). Australian marine pest monitoring manual. Version 2.0. The National System for the Prevention and Management of Marine Pest Incursions. 142 pp.

Department of Agriculture, Fisheries and Forestry (DAFF) (2012). Department of Agriculture, Fisheries and Forestry. Accessed 10 September 2012. http://www.marinepests.gov.au/national_system

GHD Pty Ltd (GHD) (2009). Darwin (AUDRW) Marine Pest Monitoring Survey Design (Report). For Northern Territory Fisheries. Department of Regional Development, Primary Industries, Fisheries and Resources. 68 pp.

Golder Associates (Golder) (2010). Darwin Harbour Marine Pest Monitoring Survey: Final Monitoring Report 2010. For the Northern Territory Department of Resources, Northern Territory Government. 24 pp.

INPEX (2012). Dredging and Spoil Disposal Management Plan - East Arm (DSDMP). INPEX Operations Australia Pty Ltd.

Maher, J., Beatty, A. and Cribb, H. (2011a). Monitoring for Marine Pests Darwin Harbour 2009-10. The Northern Territory Government Department of Resources ABN 84 085 734 992. January 2011. 33 pp.

Maher, J., Beatty, A. and Cribb, H. (2011b). Monitoring for Marine Pests Darwin Harbour 2009-10. Gove Harbour, Groote Eylandt and Melville Island. The Northern Territory Government Department of Resources ABN 84 085 734 992. January 2011. 25 pp.

McKinnon, A.D., Smit, N., Townsend, S. and Duggan, S. (2006). Darwin Harbour: Water Quality and Ecosystem Structure in a Tropical Harbour in the Early Stages of Urban Development. In: The Environment in Asia Pacific Harbours. Wolanski, E. (ed.). Springer, The Netherlands. 497 pp.

Russell, B.C. and Hewitt, C.L. (2000). Baseline Survey of the Port of Darwin for Introduced Marine Species. For the Northern Territory Government, Department of Transport and Works by CSIRO Marine Research and the Museum and Art Gallery of the Northern Territory. 42 pp.

Williams, D. and Wolanksi, E. (2003). Darwin Harbour hydrodynamics and sediment transport. In: Marine and estuarine environments of Darwin Harbour. Proceedings of the Darwin Harbour Public Presentations. Darwin Harbour Regional Plan of Management.

NIMPIS (2012a). Bugula neritina reproduction and habitat, National Introduced Marine Pest Information System, viewed 13 November 2012 <http://www.marinepests.gov.au/nimpis>.

NIMPIS (2012b). Botryllus schlosseri impacts and vectors, National Introduced Marine Pest Information System, viewed 13 November 2012 <http://www.marinepests.gov.au/nimpis>.

NIMPIS (2012c). Botrylloides leachi reproduction and habitat, National Introduced Marine Pest Information System, viewed 13 November 2012 <http://www.marinepests.gov.au/nimpis>.

NIMPIS (2012d). Didemnum vexillum general information, National Introduced Marine Pest Information System, viewed 13 November 2012 <http://www.marinepests.gov.au/nimpis>.



Stafford, H. and Willan, R.C. (2007). Is it a Pest? Introduced and naturalised marine animal species of Torres Strait Northern Australia. Queensland Department of Primary Industries and Fisheries, Cairns.

Wells, F.E., McDonald, J.I. and Huisman, J.M. (2009). Introduced Marine Species in Western Australia. Department of Fisheries Western Australia. Fisheries Occasional Publications No 57, March 2009.

Williams, D. and Wolanski, E. (2003). Darwin Harbour hydrodynamics and sediment transport. Proceedings of the Darwin Harbour Public Presentations - February 2003. Darwin Harbour Regional Plan of Management.



Ichthys Nearshore Environmental Monitoring Program

APPENDIX A LOCATION MAPS FOR B1

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1. Aerial: Bing aerials, Copyright Reserved, Microsoft 2012.2. Schematic only, not to be interpreted as an engineering design or construction drawing.3. Drawn by: HG. Reviewed by: VG.

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Coordinate System: GDA 1994 MGA Zone 52

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BLAYDIN POINT: LOCATIONS 3 AND 4 A2OCTOBER 20121278205843

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1. Aerial: Bing Aerial, Copyright Reserved, Microsoft 2012. 2. Schematic only, not to be interpreted as an engineering design or construction drawing.3. Drawn by: HG. Reviewed by: VG.


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MIDDLE ARM PIPELINE SHORE CROSSING: LOCATION 5 A3OCTOBER 20121278205843

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PROPOSED CYCLONE MOORING: LOCATION 6 A4OCTOBER 20121278205843

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EAST ARM MOORING: LOCATION 7 A5OCTOBER 20121278205843

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DREDGE DISPOSAL: LOCATION 9 A6OCTOBER 20121278205843

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CHANNEL ISLAND: LOCATION 19 A15OCTOBER 20121278205843PROJECT

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APPENDIX B GOLDER CHAIN OF CUSTODY FORM

GAIMS DOCUMENT No 16 RL0

FIELD SAMPLING CHAIN OF CUSTODY

To: ________________________ Lab quote # (if applicable): ___________________________ Date: _______________________ Golder Contact: ____________________________________ Golder job #(s): __________________ Golder Project & client: ________________________

Matrix*

Sample No / ID

Date of Collection

Site and Sample Description

Type of Analysis [

Miscellaneous Information

W SD S B O Day Month Year Time

* Note for matrix type: W = water SD = sediment S = soil B = biological O = other

TURNAROUND REPORT FORMAT TO BE COMPLETED BY LAB

Sampled By: (Name) Date Time Normal (up to 10 days) .. High (5 days) ............... Urgent

Results by email? Y N Email address: ____________________ Fax? Y N Fax number: ____________________ Post? Y N

Received By: (Name) ** Date Time

Relinquished by: (Name) Date Time

Lab job #:__________ Approx completion date: ________________

Samples received in good order? Y N Comments: _____________________________________________

** ON RECEIPT BY LABORATORY, PLEASE SIGN THIS FORM AND FAX TO GOLDER’S OFFICE: Page___of___ AUCKLAND CHRISTCHURCH DUNEDIN NELSON TAURANGA

FAX: 09 486 8072; FAX: 03 377 9944; FAX: 03 474 9642; FAX: 03 548 1727; FAX: 07 928 5336 PO Box 33-849, Takapuna 0622 PO Box 2281, Christchurch 8140 PO Box 1087, Dunedin 9054 PO Box 1724, Nelson 7040 PO Box 13611, Tauranga 3141




APPENDIX C SAMPLE COLLECTION AND SPECIMEN REGISTER 2012

Location code

Collection date

Sublocation number

Sublocation name Sublocation code

Sample method

Sample number

Waypoint 1 (WGS84) Waypoint 2 (WGS84) Sample code Taxon Fixed in Preserved in Collected by Reliquished by Transferred by Sent to Date sent Date received Taxonomist /Identifier

Remarks

DRW - 1 Module Offloading Facility MOF1 T_ 1 - - NOT SET - - - - - - - - - - -DRW - 1 Module Offloading Facility MOF1 T_ 2 - - NOT SET - - - - - - - - - - -DRW - 1 Module Offloading Facility MOF1 T_ 3 - - NOT SET - - - - - - - - - - -DRW 5/08/2012 1 Module Offloading Facility MOFA ED 1 S12 30 42.6 E130 55 44.0 S12 30 42.2 E130 55 46.5 DRW1208MOFAED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 1 Module Offloading Facility MOF1 ED 3 S12 30 42.9 E130 55 35.3 S12 30 40.3 E130 55 32.3 DISCARDED AT SORTING Crustacea - - Golder Associates - - - - - - -DRW 5/08/2012 1 Module Offloading Facility MOFA ED 3 S12 30 42.9 E130 55 35.3 S12 30 40.3 E130 55 32.3 DRW1208MOFAED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 1 Module Offloading Facility MOFA ED 3 S12 30 42.9 E130 55 35.3 S12 30 40.3 E130 55 32.3 DRW1208MOFAED_04 Bivalve 10% seawater buffered for 70% ethanol Golder Associates simplex Sunset clam MAGNT 41135 41135 Dr Richard Willan PsammobiidaeDRW 4/08/2012 1 Module Offloading Facility MOF2 ED 2 S12 30 38.3 E130 55 33.4 S12 30 40.1 E130 55 36.3 DRW1208MOF2ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 1 Module Offloading Facility MOF2 ED 2 S12 30 38.3 E130 55 33.4 S12 30 40.1 E130 55 36.3 DRW1208MOF2ED_04 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 1 Module Offloading Facility MOF1 VG 1 S12 30 45.7 E130 55 44.3 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 5/08/2012 1 Module Offloading Facility MOF1 VG 2 - - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 6/08/2012 1 Module Offloading Facility MOF1 VG 3 S12 30 46.2 E130 55 38.0 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 1 Module Offloading Facility MOF1 ZT 1 S12 30 29.3 E130 55 34.7 S12 30 28.0 E130 55 32.4 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 1 Module Offloading Facility MOF1 ZT 2 S12 30 41.4 E130 55 40.8 S12 30 41.5 E130 55 38.4 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 1 Module Offloading Facility MOF1 ZT 3 S12 31 05.3 E130 55 49.6 S12 31 05.3 E130 55 49.8 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 gymnodinioid UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__01 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__01 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__01 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__01 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 1 Module Offloading Facility MOF1 C_ 1 - - DRW1208MOF1C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 12/08/2012 1 Module Offloading Facility MOF1 BW 1 S12 30 38.6 E130 55 23.7 S12 30 44.9 E130 55 27.7 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 1 S12 30 32.6 E130 55 43.8 S12 30 37.1 E130 55 45.7 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 2 S12 30 42.0 E130 55 47.7 S12 30 44.9 E130 55 49.5 DRW1208MOF2ED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 2 S12 30 42.0 E130 55 47.7 S12 30 44.9 E130 55 49.5 DRW1208MOF2ED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno redika 0 MITS 0 0 Mike Page DidemnidaeDRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 2 S12 30 42.0 E130 55 47.7 S12 30 44.9 E130 55 49.5 DRW1208MOF2ED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno millari 0 MITS 0 0 Mike Page AscidiidaeDRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_05 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 1 Polychaete MAGNT 41135 41135 Dr Chris Glasby PolynoidaeDRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_05 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_06 Barnacles 0 0 Golder Associates/Cardno amphitrite 0 0 0 0 Rick Johnson 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_06 Barnacles 0 0 Golder Associates/Cardno rosa 0 0 0 0 Rick Johnson 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_07 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 0 MITS 0 0 Dr Dennis Gordon PetraliellidaeDRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_07 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno lata 0 MITS 0 0 Dr Rad Nair 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_07 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno aperta 0 MITS 0 0 Dr Rad Nair 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 DRW1208MOF2ED_07 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. A 0 MITS 0 0 Dr Rad Nair 0DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ED 3 S12 30 47.1 E130 55 48.7 S12 30 49.3 E130 55 52.3 NO SAMPLE COLLECTED - - - Golder Associates/Cardno - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 VG 1 S12 30 20.2 E130 55 34.2 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOFA VG 2 S12 30 40.7 E130 55 46.7 - DRW1208MOFAVG_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates redika 0 MITS 0 0 Mike Page DidemnidaeDRW 5/08/2012 2 Module Offloading Facility Dredged Area MOF2 VG 3 S12 31 03.1 E130 56 03.4 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ZT 1 S12 30 20.4 E130 55 38.1 S12 30 22.2 E130 55 39.4 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ZT 2 S12 30 34.9 E130 55 44.0 S12 30 38.6 E130 55 45.6 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 2 Module Offloading Facility Dredged Area MOF2 ZT 3 S12 31 00.8 E130 55 54.2 S12 31 01.6 E130 55 56.3 DISCARDED - - - Golder Associates - - - - - - -DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__02 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__02 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__01 Dinoflagellate - 1% Lugol's solution Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 1/08/2012 2 Module Offloading Facility Dredged Area MOF2 C_ 2 - - DRW1208MOF2C__03 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 5/08/2012 3 Product Loading Facility PLF3 ED 1 S12 30 18.9 E130 55 01.7 S12 30 16.2 E130 55 05.7 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 5/08/2012 3 Product Loading Facility PLF3 ED 3 S12 30 33.0 E130 54 39.9 S12 30 34.6 E130 54 37.3 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 ED 2 S12 30 22.4 E130 54 38.3 S12 30 20.2 E130 54 42.3 DISCARDED AT SORTING - - - Golder Associates/Cardno - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 VG 1 S12 30 36.5 E130 54 47.0 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 VG 2 S12 30 25.0 E130 54 54.1 - DISCARDED AT SORTING - - - Golder Associates - - - - - - -DRW 5/08/2012 3 Product Loading Facility PLF3 VG 3 S12 30 32.8 E130 54 36.3 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 ZT 1 S12 30 37.2 E130 54 46.7 S12 30 37.5 E130 54 47.0 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 ZT 2 S12 30 24.5 E130 54 59.4 S12 30 24.4 E130 54 59.0 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 3 Product Loading Facility PLF3 ZT 3 S12 30 30.7 E130 54 39.1 S12 30 30.4 E130 54 39.5 DISCARDED - - - Golder Associates - - - - - - -DRW 12/08/2012 3 Product Loading Facility PLF3 BW 1 S12 30 46.1 E130 54 55.0 S12 30 37.0 E130 55 10.7 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 5/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 1 S12 30 37.4 E130 54 36.8 S12 30 37.4 E130 54 34.5 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 5/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 5 S12 30 15.6 E130 55 26.6 S12 30 12.9 E130 55 31.5 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 4 S12 30 02.1 E130 54 49.9 S12 29 59.3 E130 54 51.7 DRW1208PLF4ED_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno redika 0 MITS 0 0 Mike Page DidemnidaeDRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 3 S12 30 07.7 E130 54 26.5 S12 30 09.4 E130 54 30.2 RE-SAMPLED AS ED011 - - - Golder Associates/Cardno - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 3 S12 30 07.7 E130 54 26.5 S12 30 09.4 E130 54 30.2 DRW1208PLF4ED_04 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno redika 0 MITS 0 0 Mike Page DidemnidaeDRW 4/08/2012 4 Product Loading Facility Dredged Area PLFA ED 3 S12 30 07.7 E130 54 26.5 S12 30 09.4 E130 54 30.2 DRW1208PLFAED_03 Fish 10% seawater buffered for 70% ethanol Golder Associates/Cardno madurensis Madura goby MAGNT 41135 41135 Dr Michael Hammer GobiidaeDRW 4/08/2012 4 Product Loading Facility Dredged Area PLF3 ED 2 S12 30 36.0 E130 54 18.6 S12 30 35.8 E130 54 15.2 DRW1208PLF3ED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ED 2 S12 30 22.4 E130 54 38.3 S12 30 20.2 E130 54 42.3 DRW1208PLF4ED_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno crassa Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 4/08/2012 4 Product Loading Facility Dredged Area PLFA VG 1 S12 30 44.4 E130 54 43.6 - DISCARDED Bivalve 10% seawater buffered for 70% ethanol Golder Associates - - MAGNT 41135 41135 Dr Richard Willan -DRW 5/08/2012 4 Product Loading Facility Dredged Area PLF4 VG 2 S12 30 12.8 E130 54 46.1 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 5/08/2012 4 Product Loading Facility Dredged Area PLF4 VG 3 S12 30 19.2 E130 55 05.7 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ZT 1 S12 30 44.2 E130 54 39.2 S12 30 43.7 E130 54 37.5 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ZT 2 S12 30 27.3 E130 54 22.6 S12 30 27.7 E130 54 21.0 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ZT 3 S12 30 15.8 E130 54 42.5 S12 30 16.3 E130 54 41.6 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 ZT 4 S12 30 11.2 E130 55 04.2 S12 30 10.9 E130 55 04.5 DISCARDED - - - Golder Associates - - - - - - -DRW 12/08/2012 4 Product Loading Facility Dredged Area PLF4 BW 1 0 0 DRW1208PLF4BW_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 4/08/2012 4 Product Loading Facility Dredged Area PLF4 BW 1 - - DRW1208PLF4BW_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates donaciformis Wafer clam MAGNT 41135 41135 Dr Richard Willan TellinidaeDRW 5/08/2012 5 Middle Arm Pipeline MAPI ED 1 S12 31 55.3 E130 51 51.3 S12 31 53.9 E130 51 48.9 DISCARDED AT SORTING - - - Golder Associates - - - - - - -DRW 5/08/2012 5 Middle Arm Pipeline MAPI ED 2 S12 32 00.1 E130 51 57.6 S12 31 58.5 E130 51 55.3 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 5/08/2012 5 Middle Arm Pipeline MAPI ED 3 S12 32 01.6 E130 51 58.9 S12 32 00.1 E130 51 57.2 DISCARDED AT SORTING - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI VG 1 S12 31 35.9 E130 52 02.1 - DRW1208MAPIVG_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates coxi Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 4/08/2012 5 Middle Arm Pipeline MAPI VG 2 S12 31 44.1 E130 52 16.8 - DRW1208MAPIVG_02 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 5 Middle Arm Pipeline MAPI VG 3 S12 31 55.0 E130 52 19.4 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI VG 4 S12 31 53.0 E130 52 28.2 - DRW1208MAPIVG_01 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 5 Middle Arm Pipeline MAPI VG 5 S12 32 19.2 E130 52 29.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI ZT 1 S12 31 46.4 E130 51 57.8 S12 31 44.8 E130 51 57.7 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI ZT 2 S12 31 55.7 E130 52 11.0 S12 31 55.7 E130 52 11.7 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI ZT 3 S12 31 50.2 E130 52 15.7 S12 31 50.1 E130 52 13.5 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 5 Middle Arm Pipeline MAPI ZT 4 S12 32 09.7 E130 52 08.6 S12 32 07.8 E130 52 03.9 DISCARDED - - - Golder Associates - - - - - - -DRW 11/08/2012 6 Cyclone Mooring CYMO ED 6 S12 36 00.3 E130 52 48.1 S12 36 01.7 E130 52 44.4 DRW1208CYMOED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 6 Cyclone Mooring CYMO ED 5 S12 36 00.5 E130 52 39.4 S12 36 03.4 E130 52 36.0 DISCARDED Animal 10% seawater buffered for 70% ethanol Golder Associates - - RBGS 41136 0 Prof Alan Millar -DRW 11/08/2012 6 Cyclone Mooring CYMO ED 4 S12 36 08.4 E130 52 31.4 S12 36 09.4 E130 52 27.8 DRW1208CYMOED_06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates fragilis Theora clam MAGNT 41135 41135 Dr Richard Willan SemelidaeDRW 11/08/2012 6 Cyclone Mooring CYMO ED 3 S12 35 34.5 E130 53 05.7 S12 35 33.0 E130 53 09.8 DRW1208CYMOED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 6 Cyclone Mooring CYMO ED 3 S12 35 34.5 E130 53 05.7 S12 35 33.0 E130 53 09.8 DRW1208CYMOED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 6 Cyclone Mooring CYMO ED 2 S12 35 18.6 E130 52 59.8 S12 35 17.0 E130 52 57.2 DRW1208CYMOED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 6 Cyclone Mooring CYMO ED 1 S12 34 56.1 E130 52 51.8 S12 34 52.8 E130 52 51.4 DRW1208CYMOED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 7 East Arm Mooring EAMO ED 1 S12 29 47.2 E130 53 53.4 S12 29 47.8 E130 53 50.0 DRW1208EAMOED_06 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates sp. 0 MITS 0 0 Mike Page DidemnidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 1 S12 29 47.2 E130 53 53.4 S12 29 47.8 E130 53 50.0 DRW1208EAMOED_07 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates millari 0 MITS 0 0 Mike Page AscidiidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 2 S12 29 47.9 E130 53 56.8 S12 29 47.8 E130 53 53.4 DRW1208EAMOED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 7 East Arm Mooring EAMO ED 3 S12 29 44.7 E130 53 57.0 S12 29 44.9 E130 53 53.2 DRW1208EAMOED_08 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates stuhlmanni 0 MITS 0 0 Mike Page StyelidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 3 S12 29 44.7 E130 53 57.0 S12 29 44.9 E130 53 53.2 DRW1208EAMOED_09 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates 0 MITS 0 0 Mike Page StyelidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 3 S12 29 44.7 E130 53 57.0 S12 29 44.9 E130 53 53.2 DRW1208EAMOED_10 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 7 East Arm Mooring EAMO ED 4 S12 29 35.6 E130 54 00.0 S12 29 36.5 E130 53 56.7 DRW1208EAMOED_03 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 7 East Arm Mooring EAMO ED 4 S12 29 35.6 E130 54 00.0 S12 29 36.5 E130 53 56.7 DRW1208EAMOED_04 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates cf. cyanea 0 MITS 0 0 Mike Page HolozoidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 5 S12 29 37.0 E130 54 00.0 S12 29 37.3 E130 53 56.8 DRW1208EAMOED_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates translucidum 0 MITS 0 0 Mike Page DidemnidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 5 S12 29 37.0 E130 54 00.0 S12 29 37.3 E130 53 56.8 DRW1208EAMOED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates intestinata 0 MITS 0 0 Mike Page StyelidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 5 S12 29 37.0 E130 54 00.0 S12 29 37.3 E130 53 56.8 DRW1208EAMOED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates listerianum 0 MITS 0 0 Mike Page DidemnidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 5 S12 29 37.0 E130 54 00.0 S12 29 37.3 E130 53 56.8 DRW1208EAMOED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates millari 0 MITS 0 0 Mike Page AscidiidaeDRW 7/08/2012 7 East Arm Mooring EAMO ED 5 S12 29 37.0 E130 54 00.0 S12 29 37.3 E130 53 56.8 DRW1208EAMOED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates cf. cyanea 0 MITS 0 0 Mike Page HolozoidaeDRW 7/08/2012 7 East Arm Mooring EAMO VG 1 S12 29 46.3 E130 53 53.7 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 7 East Arm Mooring EAMO VG 2 S12 29 48.0 E130 53 54.3 - DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ED 1 S12 15 03.0 E130 48 29.9 S12 15 00.3 E130 48 27.9 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ED 2 S12 14 40.1 E130 48 28.1 S12 14 42.2 E130 48 24.5 DISCARDED AT SORTING - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ED 3 S12 14 58.6 E130 48 25.4 S12 14 58.2 E130 48 22.2 DISCARDED AT SORTING - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ED 4 S12 14 42.4 E130 48 35.0 S12 14 42.1 E130 48 31.6 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ED 5 S12 14 48.0 E130 48 16.5 S12 14 48.3 E130 48 12.1 DRW1208DSDSED_01 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 1 S12 14 52.4 E130 48 29.6 - DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 2 S12 14 43.1 E130 48 21.7 - DRW1208DSDSVG_02 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 2 S12 14 43.1 E130 48 21.7 - DRW1208DSDSVG_02 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 3 S12 14 47.4 E130 48 40.5 - DRW1208DSDSVG_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno impolitum True cockle MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 4 S12 14 54.1 E130 48 15.3 - DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS VG 5 S12 15 03.6 E130 48 30.2 - DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -

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DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ZP 1 S12 15 03.0 E130 48 29.9 S12 15 01.6 E130 48 29.0 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ZT 2 S12 14 40.1 E130 48 28.1 S12 14 41.2 E130 48 26.7 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ZT 3 S12 14 58.6 E130 48 25.4 S12 14 58.3 E130 48 23.7 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ZT 4 S12 14 42.4 E130 48 35.0 S12 14 42.3 E130 48 33.0 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 9 Dredge Spoil Disposal Site DSDS ZT 5 S12 14 48.0 E130 48 16.5 S12 14 48.3 E130 48 14.2 DISCARDED ON SITE - - - Golder Associates/Cardno - - - - - - -DRW 6/08/2012 10 Charlie 1 CHL1 ED 2 S12 28 56.1 E130 49 21.8 S12 28 52.7 E130 49 18.7 DRW1208CHL1ED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 1 S12 28 52.9 E130 49 20.2 S12 28 49.3 E130 49 16.6 DRW1208CHL1ED_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 1 S12 28 52.9 E130 49 20.2 S12 28 49.3 E130 49 16.6 DRW1208CHL1ED_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 3 S12 28 56.4 E130 49 09.4 S12 28 53.1 E130 49 06.3 DRW1208CHL1ED_09 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 4 S12 28 53.9 E130 49 20.0 S12 28 49.2 E130 49 15.7 DISCARDED Crustacea - - Golder Associates - - - - - - -DRW 6/08/2012 10 Charlie 1 CHL1 ED 5 S12 28 47.8 E130 49 37.9 S12 28 43.9 E130 49 32.7 DRW1208CHL1ED_04 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cf. darwinensis Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 6/08/2012 10 Charlie 1 CHL1 ED 5 S12 28 47.8 E130 49 37.9 S12 28 43.9 E130 49 32.7 DRW1208CHL1ED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 5 S12 28 47.8 E130 49 37.9 S12 28 43.9 E130 49 32.7 DRW1208CHL1ED_07 Bivalve 10% seawater buffered for 70% ethanol Golder Associates darwinensis Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 6/08/2012 10 Charlie 1 CHL1 ED 5 S12 28 47.8 E130 49 37.9 S12 28 43.9 E130 49 32.7 DRW1208CHL1ED_08 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 6 S12 28 56.5 E130 49 29.0 S12 28 52.6 E130 49 24.8 DRW1208CHL1ED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 ED 6 S12 28 56.5 E130 49 29.0 S12 28 52.6 E130 49 24.8 DRW1208CHL1ED_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates semirugata Venus clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 6/08/2012 10 Charlie 1 CHL1 VG 1 S12 28 55.7 E130 49 24.5 - DISCARDED Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 0 0DRW 6/08/2012 10 Charlie 1 CHL1 VG 2 S12 29 00.0 E130 49 34.0 - DRW1208CHL1VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 VG 2 S12 29 00.0 E130 49 34.0 - DRW1208CHL1VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 VG 3 S12 29 01.8 E130 49 17.4 - DRW1208CHL1VG_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 10 Charlie 1 CHL1 VG 4 S12 28 47.0 E130 49 33.0 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 6/08/2012 10 Charlie 1 CHL1 VG 5 S12 28 48.8 E130 49 16.5 - DRW1208CHL1VG_04 Bivalve 10% seawater buffered for 70% ethanol Golder Associates macgillivrayi Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 6/08/2012 10 Charlie 1 CHL1 VG 5 S12 28 48.8 E130 49 16.5 - DRW1208CHL1VG_04 Bivalve 10% seawater buffered for 70% ethanol Golder Associates micropterus Mussel MAGNT 41135 41135 Dr Richard Willan MytilidaeDRW 6/08/2012 10 Charlie 1 CHL1 VG 5 S12 28 48.8 E130 49 16.5 - DRW1208CHL1VG_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 13/08/2012 10 Charlie 1 CHL1 P_ 1 - - DISCARDED Crustacea - - Golder Associates/Cardno - - - - - - -DRW 13/08/2012 10 Charlie 1 CHL1 P_ 1 - - DRW1208CHL1P__01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno tuberculosa Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 13/08/2012 10 Charlie 1 CHL1 P_ 1 - - DRW1208CHL1P__02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 11 Charlie 2 CHL2 ED 1 S12 29 30.6 E130 49 39.8 S12 29 27.6 E130 49 38.6 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 ED 2 S12 29 26.6 E130 49 43.2 S12 29 22.4 E130 49 40.3 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 ED 3 S12 29 30.2 E130 49 47.3 S12 29 27.9 E130 49 45.0 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 ED 4 S12 29 21.9 E130 49 56.3 S12 29 17.6 E130 49 52.4 DRW1208CHL2ED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 11 Charlie 2 CHL2 ED 5 S12 29 37.6 E130 49 50.2 S12 29 36.5 E130 49 50.2 DRW1208CHL2ED_01 Crustacea 10% seawater buffered for 70% ethanol Golder Associates Native 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 11 Charlie 2 CHL2 ED 6 S12 29 28.4 E130 49 29.7 S12 29 24.8 E130 49 25.4 DRW1208CHL2ED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 11 Charlie 2 CHL2 ED 6 S12 29 28.4 E130 49 29.7 S12 29 24.8 E130 49 25.4 DRW1208CHL2ED_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cultellus Jacknife clam MAGNT 41135 41135 Dr Richard Willan PharidaeDRW 6/08/2012 11 Charlie 2 CHL2 VG 1 S12 29 20.6 E130 49 34.7 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 VG 2 S12 29 29.2 E130 49 42.8 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 VG 3 S12 29 20.2 E130 49 51.3 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 VG 4 S12 29 32.9 E130 49 52.8 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 11 Charlie 2 CHL2 VG 5 S12 29 32.6 E130 49 34.7 - DISCARDED - - - Golder Associates - - - - - - -DRW 13/08/2012 11 Charlie 2 CHL2 P_ 1 S12 29 20.7 E130 49 26.1 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 6/08/2012 12 Charlie 7 CHL2 ED 1 S12 29 30.6 E130 49 39.8 S12 29 27.6 E130 49 38.6 DRW1208CHL2ED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 1 S12 28 55.6 E130 50 52.6 S12 28 56.4 E130 50 57.1 DRW1208CHL7ED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 1 S12 28 55.6 E130 50 52.6 S12 28 56.4 E130 50 57.1 DRW1208CHL7ED_08 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 2 S12 28 58.6 E130 50 53.7 S12 28 58.8 E130 50 57.4 DRW1208CHL7ED_09 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 4 S12 28 50.0 E130 51 10.6 S12 28 51.6 E130 51 15.7 DRW1208CHL7ED_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cultellus Jacknife clam MAGNT 41135 41135 Dr Richard Willan PharidaeDRW 6/08/2012 12 Charlie 7 CHL7 ED 4 S12 28 50.0 E130 51 10.6 S12 28 51.6 E130 51 15.7 DRW1208CHL7ED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 4 S12 28 50.0 E130 51 10.6 S12 28 51.6 E130 51 15.7 DRW1208CHL7ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 4 S12 28 50.0 E130 51 10.6 S12 28 51.6 E130 51 15.7 DRW1208CHL7ED_04 Bivalve 10% seawater buffered for 70% ethanol Golder Associates darwinensis Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 6/08/2012 12 Charlie 7 CHL7 ED 5 S12 29 03.0 E130 50 43.5 S12 29 03.7 E130 50 47.7 DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 6/08/2012 12 Charlie 7 CHL7 ED 6 S12 29 04.9 E130 51 04.0 S12 29 06.3 E130 51 09.2 DRW1208CHL7ED_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 6 S12 29 04.9 E130 51 04.0 S12 29 06.3 E130 51 09.2 DRW1208CHL7ED_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 ED 6 S12 29 04.9 E130 51 04.0 S12 29 06.3 E130 51 09.2 DRW1208CHL7ED_07 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cf. soleneae Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 6/08/2012 12 Charlie 7 CHL7 VG 1 S12 28 58.3 E130 50 56.4 - DISCARDED Crustacea - - Golder Associates - - - - - - -DRW 6/08/2012 12 Charlie 7 CHL7 VG 2 S12 29 03.4 E130 50 49.9 - DISCARDED ON SITE - - - Golder Associates - - - - - - -DRW 6/08/2012 12 Charlie 7 CHL7 VG 3 S12 29 02.1 E130 51 05.9 - DRW1208CHL7VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 VG 5 S12 28 52.1 E130 50 50.7 - DRW1208CHL7VG_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 12 Charlie 7 CHL7 VG 4 S12 28 50.4 E130 51 10.2 - DISCARDED AT SORTING - - - Golder Associates - - - - - - -DRW 13/08/2012 12 Charlie 7 CHL7 P_ 1 S12 28 56.9 E130 51 27.8 - DRW1208CHL7P__01 Algae 10% seawater buffered for 70% ethanol Golder Associates sp. 0 RBGS 41136 0 Prof Alan Millar 0DRW 5/08/2012 13 Bravo 5 BRV5 ED 4 0 - DRW1208BRV5ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 13 Bravo 5 BRV5 ED 4 0 - DRW1208BRV5ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno rosa 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 13 Bravo 5 BRV5 ED 1 S12 27 45.5 E130 47 15.2 S12 27 49.6 E130 47 18.0 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 13 Bravo 5 BRV5 ED 2 S12 27 38.5 E130 47 10.5 S12 27 44.5 E130 47 12.5 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 13 Bravo 5 BRV5 ED 3 S12 27 37.9 E130 47 04.1 S12 27 42.0 E130 47 05.6 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 13 Bravo 5 BRV5 ED 4 S12 27 34.8 E130 47 02.2 S12 27 42.0 E130 47 03.5 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 13 Bravo 5 BRV5 ED 5 S12 27 39.7 E130 46 52.6 S12 27 44.4 E130 46 53.6 DRW1208BRV5ED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 13 Bravo 5 BRV5 ED 5 S12 27 39.7 E130 46 52.6 S12 27 44.4 E130 46 53.6 DRW1208BRV5ED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 13 Bravo 5 BRV5 ED 6 S12 27 31.5 E130 47 22.3 S12 27 36.3 E130 47 22.7 DRW1208BRV5ED_01 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 MITS 0 0 Dr Shane Ahyong ArchaeobalanidaeDRW 5/08/2012 13 Bravo 5 BRV5 VG 3 S12 27 44.1 E130 46 59.1 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 6/08/2012 13 Bravo 5 BRV5 VG 5 S12 27 31.1 E130 47 14.7 - DRW1208BRV5VG_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates valtonis Wafer clam MAGNT 41135 41135 Dr Richard Willan TellinidaeDRW 6/08/2012 13 Bravo 5 BRV5 VG 5 S12 27 31.1 E130 47 14.7 - DRW1208BRV5VG_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 13 Bravo 5 BRV5 VG 5 S12 27 31.1 E130 47 14.7 - DRW1208BRV5VG_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 13 Bravo 5 BRV5 VG 4 S12 27 29.5 E130 46 59.3 - DRW1208BRV5VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 13 Bravo 5 BRV5 VG 4 S12 27 29.5 E130 46 59.3 - DRW1208BRV5VG_01 Barnacles ` 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 13 Bravo 5 BRV5 VG 1 S12 27 37.1 E130 47 06.8 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 6/08/2012 13 Bravo 5 BRV5 VG 2 S12 27 41.0 E130 47 16.0 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 14 Bravo 2 BRV2 ED 1 S12 22 54.2 E130 45 43.6 S12 22 54.7 E130 45 39.4 DRW1208BRV2ED_08 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno spiratulus Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 2 S12 23 03.8 E130 45 34.7 S12 23 03.7 E130 45 29.9 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno valtonis Wafer clam MAGNT 41135 41135 Dr Richard Willan TellinidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_03 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby EunicidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_03 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Mangrove "worm" MAGNT 41135 41135 Dr Chris Glasby Mollusca: TeredinidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_03 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_03 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SyllidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 3 S12 23 03.9 E130 45 27.6 S12 23 04.7 E130 45 22.6 DRW1208BRV2ED_04 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno redika 0 MITS 0 0 Mike Page DidemnidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 4 S12 23 00.1 E130 45 26.1 S12 23 01.7 E130 45 22.0 DRW1208BRV2ED_01 Crustacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 14 Bravo 2 BRV2 ED 5 S12 23 06.7 E130 45 17.8 S12 23 09.1 E130 45 13.8 DRW1208BRV2ED_07 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Mangrove "worm" MAGNT 41135 41135 Dr Chris Glasby Mollusca: TeredinidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 6 S12 23 07.1 E130 45 17.0 S12 23 10.4 E130 45 14.1 DISCARDED Crustacea - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 14 Bravo 2 BRV2 ED 6 S12 23 07.1 E130 45 17.0 S12 23 10.4 E130 45 14.1 DRW1208BRV2ED_06 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 5/08/2012 14 Bravo 2 BRV2 ED 6 S12 23 07.1 E130 45 17.0 S12 23 10.4 E130 45 14.1 DRW1208BRV2ED_06 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno neritina 0 MITS 0 0 Dr Rad Nair 0DRW 5/08/2012 14 Bravo 2 BRV2 ED 6 S12 23 07.1 E130 45 17.0 S12 23 10.4 E130 45 14.1 DRW1208BRV2ED_06 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno tenuirostris (?) 0 MITS 0 0 Dr Rad Nair 0DRW 5/08/2012 14 Bravo 2 BRV2 ED 6 S12 23 07.1 E130 45 17.0 S12 23 10.4 E130 45 14.1 DRW1208BRV2ED_06 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. A 0 MITS 0 0 Dr Rad Nair 0DRW 5/08/2012 14 Bravo 2 BRV2 VG 1 S12 23 02.5 E130 45 29.5 - DRW1208BRV2VG_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Peanut worm MAGNT 41135 41135 Dr Chris Glasby SipunculaDRW 5/08/2012 14 Bravo 2 BRV2 VG 2 S12 22 54.9 E130 45 21.4 - DRW1208BRV2VG_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno valtonis Wafer clam MAGNT 41135 41135 Dr Richard Willan TellinidaeDRW 5/08/2012 14 Bravo 2 BRV2 VG 3 S12 23 08.0 E130 45 22.3 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 5/08/2012 14 Bravo 2 BRV2 VG 4 S12 23 06.6 E130 45 39.9 - DRW1208BRV2VG_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno rosa 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 14 Bravo 2 BRV2 VG 5 S12 22 53.0 E130 45 38.3 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 13/08/2012 15 Weed Reef WDRF DV 1 S12 30 16.8 E130 48 35.9 - DRW1208WDRFDV_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno vulsella Sponge-finger oyster MAGNT 41135 41135 Dr Richard Willan MalleidaeDRW 13/08/2012 15 Weed Reef WDRF DV 2 S12 29 58.3 E130 48 26.2 - DRW1208WDRFDV_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno vulsella Sponge-finger oyster MAGNT 41135 41135 Dr Richard Willan MalleidaeDRW 13/08/2012 15 Weed Reef WDRF DV 2 S12 29 58.3 E130 48 26.2 - DRW1208WDRFDV_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno orientalis Polychaete MAGNT 41135 41135 Dr Chris Glasby SabellidaeDRW 13/08/2012 15 Weed Reef WDRF DV 2 S12 29 58.3 E130 48 26.2 - DRW1208WDRFDV_04 Algae 10% seawater buffered for 70% ethanol Golder Associates/Cardno lentillifera 0 RBGS 41136 0 Prof Alan Millar 0DRW 13/08/2012 15 Weed Reef WDRF DV 2 S12 29 58.3 E130 48 26.2 - DRW1208WDRFDV_04 Algae 10% seawater buffered for 70% ethanol Golder Associates/Cardno racemosa var. lamou0 RBGS 41136 0 Prof Alan Millar 0DRW 13/08/2012 15 Weed Reef WDRF DV 2 S12 29 58.3 E130 48 26.2 - DRW1208WDRFDV_04 Algae 10% seawater buffered for 70% ethanol Golder Associates/Cardno floresii 0 RBGS 41136 0 Prof Alan Millar 0DRW 6/08/2012 15 Weed Reef WDRF ZT 1 S12 29 18.8 E130 48 00.8 S12 29 17.9 E130 47 56.0 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 15 Weed Reef WDRF ZT 2 S12 29 18.0 E130 47 44.8 S12 29 18.7 E130 47 46.3 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 15 Weed Reef WDRF ZT 3 S12 30 03.5 E130 48 23.3 S12 30 00.5 E130 48 18.0 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 15 Weed Reef WDRF ZT 4 S12 30 07.3 E130 47 59.1 S12 30 02.8 E130 47 56.1 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 15 Weed Reef WDRF ZT 5 S12 29 43.8 E130 48 19.2 S12 29 38.1 E130 48 19.2 DISCARDED - - - Golder Associates - - - - - - -DRW 10/08/2012 16 East Arm Wharf EAWF C_ 1 - - DRW1208EAWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 10/08/2012 16 East Arm Wharf EAWF C_ 1 - - DRW1208EAWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 10/08/2012 16 East Arm Wharf EAWF C_ 1 - - DRW1208EAWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 10/08/2012 16 East Arm Wharf EAWF C_ 1 - - DRW1208EAWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_01 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 1 Polychaete MAGNT 41135 41135 Dr Chris Glasby SabellidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_01 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 1 Polychaete MAGNT 41135 41135 Dr Chris Glasby EunicidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_01 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 1 Polychaete MAGNT 41135 41135 Dr Chris Glasby EunicidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno sandvichensis Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno perversa Sphenia clam MAGNT 41135 41135 Dr Richard Willan MyidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno sp. 1 Polychaete MAGNT 41135 41135 Dr Chris Glasby TerebellidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno stellatus Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno bispiralis Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby EunicidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_04 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno versicolor 0 MITS 0 0 Mike Page DidemnidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_05 Algae 10% seawater buffered for 70% ethanol Golder Associates/Cardno prophyra 0 RBGS 41136 0 Prof Alan Millar 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 1 S12 29 23.3 E130 52 48.2 - DRW1208EAWFDV_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DISCARDED Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MAGNT 41135 41135 0 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_07 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amaryllis 0 MITS 0 0 Dr Shane Ahyong ArchaeobalanidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_08 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno cuspidis 0 MITS 0 0 Mike Page HolozoidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_08 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno translucidum 0 MITS 0 0 Mike Page HolozoidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_09 Crab 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_09 Crab 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno afra Ark clam MAGNT 41135 41135 Dr Richard Willan ArcidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno malaccanus Date mussel MAGNT 41135 41135 Dr Richard Willan MytilidaeDRW 10/08/2012 16 East Arm Wharf EAWF DV 2 S12 29 19.8 E130 52 43.5 - DRW1208EAWFDV_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno cucullata Black-lipped rock oysMAGNT 41135 41135 Dr Richard Willan Ostreidae

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Remarks

DRW 7/08/2012 16 East Arm Wharf EAWF ED 1 S12 29 20.9 E130 52 36.0 S12 29 23.9 E130 52 35.7 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF ED 2 S12 29 28.8 E130 52 44.8 S12 29 31.1 E130 52 45.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF ED 3 S12 29 09.1 E130 52 41.5 S12 29 09.4 E130 52 43.0 DRW1208EAWFED_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 7/08/2012 16 East Arm Wharf EAWF ED 3 S12 29 09.1 E130 52 41.5 S12 29 09.4 E130 52 43.0 DRW1208EAWFED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF ED 4 S12 29 36.8 E130 52 58.2 S12 29 38.5 E130 53 00.0 DRW1208EAWFED_03 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF ED 4 S12 29 36.8 E130 52 58.2 S12 29 38.5 E130 53 00.0 DRW1208EAWFED_03 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF ED 5 S12 29 39.0 E130 52 49.4 S12 29 41.9 E130 52 48.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF VG 1 S12 29 15.5 E130 52 35.7 - DRW1208EAWFVG_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates 0 MITS 0 0 Mike Page DidemnidaeDRW 7/08/2012 16 East Arm Wharf EAWF VG 1 S12 29 15.5 E130 52 35.7 - DRW1208EAWFVG_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates 0 MITS 0 0 Mike Page StyelidaeDRW 7/08/2012 16 East Arm Wharf EAWF VG 2 S12 29 24.9 E130 52 48.6 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF VG 3 S12 29 12.7 E130 52 43.0 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF VG 4 S12 29 30.6 E130 52 58.1 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF VG 5 S12 29 33.4 E130 52 55.4 - DRW1208EAWFVG_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF ZT 1 S12 29 18.2 E130 52 38.0 S12 29 17.3 E130 52 36.5 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF ZT 2 S12 29 34.0 E130 52 56.8 S12 29 33.0 E130 52 55.4 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF ZT 3 S12 29 08.3 E130 52 40.6 S12 29 09.9 E130 52 40.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF ZT 4 S12 29 38.2 E130 53 00.2 S12 29 37.9 E130 53 01.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__07 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 Cardno 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__11 Barnacles 10% seawater buffered for 70% ethanol Golder Associates tintinnabulum 0 Cardno 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DISCARDED Bivalve - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DISCARDED Sponge 10% seawater buffered for 70% ethanol Golder Associates - - RBGS 41136 0 Prof Alan Millar -DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__04 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__05 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DISCARDED Bivalve - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__08 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__09 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates leachi 0 MITS 0 0 Mike Page BotryllinaeDRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__10 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DISCARDED Crustacea - - Golder Associates - - - - - - -DRW 7/08/2012 16 East Arm Wharf EAWF P_ 1 S12 29 28.8 E130 52 55.3 S12 29 27.6 E130 52 53.9 DRW1208EAWFP__02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 2 S12 29 18.1 E130 52 41.1 S12 29 18.0 E130 52 41.1 DRW1208EAWFP__03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 16 East Arm Wharf EAWF P_ 3 S12 29 12.4 E130 52 44.0 - DRW1208EAWFP__12 Barnacles 10% seawater buffered for 70% ethanol Golder Associates reticulatus 0 MITS 0 0 Rick Johnson 0DRW 12/08/2012 16 East Arm Wharf EAWF BW 1 S12 29 19.8 E130 52 43.3 S12 29 14.4 E130 52 37.2 DRW1208EAWFBW_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cucullata Black-lipped rock oysMAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 3/08/2012 & 6/0 18 Hudson Creek HDCK C_ 2 S12 28 50.7 E130 55 47.0 - DRW1208HDCKC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 4/08/2012 18 Hudson Creek HDCK ED 1 S12 28 55.5 E130 55 44.0 S12 28 53.1 E130 55 45.8 DRW1208HDCKED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 4/08/2012 18 Hudson Creek HDCK ED 1 S12 28 55.5 E130 55 44.0 S12 28 53.1 E130 55 45.8 DRW1208HDCKED_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno darwinensis Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 4/08/2012 18 Hudson Creek HDCK ED 1 S12 28 55.5 E130 55 44.0 S12 28 53.1 E130 55 45.8 DRW1208HDCKED_04 Gastropod 10% seawater buffered for 70% ethanol Golder Associates/Cardno trigonus Rock snail MAGNT 41135 41135 Dr Richard Willan MuricidaeDRW 4/08/2012 18 Hudson Creek HDCK ED 2 S12 28 45.5 E130 55 53.8 S12 28 44.4 E130 55 55.1 DRW1208HDCKED_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno cf. darwinensis Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 4/08/2012 18 Hudson Creek HDCK ED 3 S12 28 36.3 E130 56 02.4 S12 28 35.3 E130 56 05.5 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 4/08/2012 18 Hudson Creek HDCK ED 4 S12 28 55.0 E130 55 51.2 S12 28 52.5 E130 55 53.3 DRW1208HDCKED_05 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno crassa Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 6/08/2012 18 Hudson Creek HDCK VG 1 S12 28 49.6 E130 55 45.0 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 18 Hudson Creek HDCK VG 2 S12 28 54.6 E130 55 52.4 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 18 Hudson Creek HDCK VG 3 S12 28 43.1 E130 56 01.6 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 18 Hudson Creek HDCK VG 4 S12 28 43.7 E130 55 51.3 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 18 Hudson Creek HDCK VG 5 S12 28 38.0 E130 55 57.3 - DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__02 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 4/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__02 Dinoflagellate - 1% Lugol's solution Cardno oblongum 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__03 Dinoflagellate - 1% Lugol's solution Cardno conicum 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__03 Dinoflagellate - 1% Lugol's solution Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 3/08/2012 18 Hudson Creek HDCK C_ 1 - - DRW1208HDCKC__04 Dinoflagellate - 1% Lugol's solution Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 4/08/2012 18 Hudson Creek HDCK ZT 1 S12 29 04.8 E130 55 41.0 S12 29 01.8 E130 55 42.5 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 18 Hudson Creek HDCK ZT 2 S12 28 48.9 E130 55 52.8 S12 28 51.1 E130 55 51.2 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 18 Hudson Creek HDCK ZT 3 S12 28 45.8 E130 55 57.3 S12 28 45.1 E130 55 58.4 DISCARDED - - - Golder Associates - - - - - - -DRW 4/08/2012 18 Hudson Creek HDCK ZT 4 - - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 18 Hudson Creek HDCK P_ 1 S12 28 48.6 E130 55 45.5 - DRW1208HDCKP__01 Gastropod 10% seawater buffered for 70% ethanol Golder Associates trigonus Rock snail MAGNT 41135 41135 Dr Richard Willan MuricidaeDRW 7/08/2012 18 Hudson Creek HDCK P_ 1 S12 28 48.6 E130 55 45.5 - DRW1208HDCKP__02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 12/08/2012 18 Hudson Creek HDCK BW 1 S12 28 43.1 E130 55 52.7 S12 28 51.3 E130 55 43.8 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 12/08/2012 18 Hudson Creek HDCK BW 2 S12 28 33.9 E130 56 01.4 S12 28 40.7 E130 55 54.0 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 12/08/2012 18 Hudson Creek HDCK BW 3 S12 28 45.2 E130 56 00.1 S12 28 38.8 E130 56 06.2 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 7/08/2012 18 Hudson Creek HDCK BW 4 S12 29 01.2 E130 55 47.0 S12 28 54.9 E130 55 52.4 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 3/08/2012 18 Hudson Creek HDCK C_ 3 - - DRW1208HDCKC__05 Dinoflagellate - 1% Lugol's solution Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 3/08/2012 18 Hudson Creek HDCK C_ 3 - - DRW1208HDCKC__07 Dinoflagellate - 1% Lugol's solution Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 3/08/2012 18 Hudson Creek HDCK C_ 3 - - DRW1208HDCKC__06 Dinoflagellate - 1% Lugol's solution Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 6/08/2012 18 Hudson Creek HDCK VG 0 0 - DRW1208HDCKVG_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates hiantina Hiant venus clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 6/08/2012 18 Hudson Creek HDCK VG 0 0 - DRW1208HDCKVG_03 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 18 Hudson Creek HDCK VG 0 0 - DRW1208HDCKVG_04 Gastropod 10% seawater buffered for 70% ethanol Golder Associates trigonus Rock snail MAGNT 41135 41135 Dr Richard Willan MuricidaeDRW 6/08/2012 18 Hudson Creek HDCK VG 0 0 - DRW1208HDCKVG_05 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 18 Hudson Creek HDCK VG 0 0 - DRW1208HDCKVG_06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates crassa Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 11/08/2012 19 Channel Island CHIS C_ 1 S12 33 26.6 E130 51 37.7 - DRW1208CHISC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno - - UOT 0 0 Dr Gustaaf Hallegrae-DRW 11/08/2012 19 Channel Island CHIS DV 1 S12 33 14.1 E130 52 22.3 S12 33 14.2 E130 52 29.6 DRW1208CHISDV_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amaryllis 0 MITS 0 0 Dr Shane Ahyong ArchaeobalanidaeDRW 11/08/2012 19 Channel Island CHIS DV 1 S12 33 14.1 E130 52 22.3 S12 33 14.2 E130 52 29.6 DRW1208CHISDV_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno - - MITS 0 0 Mike Page -DRW 11/08/2012 19 Channel Island CHIS DV 1 S12 33 14.1 E130 52 22.3 S12 33 14.2 E130 52 29.6 DRW1208CHISDV_03 Gastropod 10% seawater buffered for 70% ethanol Golder Associates/Cardno margariticola Rock snail MAGNT 41135 41135 Dr Richard Willan MuricidaeDRW 5/08/2012 19 Channel Island CHIS ED 1 S12 33 16.0 E130 52 23.1 S12 33 15.9 E130 52 29.4 DRW1208CHISED_02 Gastropod 10% seawater buffered for 70% ethanol Golder Associates margariticola Rock snail MAGNT 41135 41135 Dr Richard Willan MuricidaeDRW 5/08/2012 19 Channel Island CHIS ED 1 S12 33 16.0 E130 52 23.1 S12 33 15.9 E130 52 29.4 DRW1208CHISED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates schlosseri 0 MITS 0 0 Mike Page StyelidaeDRW 5/08/2012 19 Channel Island CHIS ED 1 S12 33 16.0 E130 52 23.1 S12 33 15.9 E130 52 29.4 DRW1208CHISED_02 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates sp. 0 MITS 0 0 Mike Page DidemnidaeDRW 5/08/2012 19 Channel Island CHIS ED 3 S12 33 18.3 E130 51 39.3 S12 33 14.9 E130 51 39.2 DISCARDED Bivalve - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ED 4 S12 32 50.9 E130 51 31.2 S12 32 46.6 E130 51 31.9 DISCARDED Polychaete - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ED 4 S12 32 50.9 E130 51 31.2 S12 32 46.6 E130 51 31.9 DRW1208CHISED_05 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cultellus Jacknife clam MAGNT 41135 41135 Dr Richard Willan PharidaeDRW 5/08/2012 19 Channel Island CHIS ED 5 S12 32 26.0 E130 52 12.4 S12 32 23.6 E130 52 10.3 DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS VG 1 S12 32 58.2 E130 52 31.1 - DISCARDED Polychaete - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS VG 1 S12 32 58.2 E130 52 31.1 - DRW1208CHISVG_01 Algae 10% seawater buffered for 70% ethanol Golder Associates sp. 0 RBGS 41136 0 Prof Alan Millar 0DRW 5/08/2012 19 Channel Island CHIS VG 2 S12 32 59.0 E130 52 30.2 - DRW1208CHISVG_03 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 5/08/2012 19 Channel Island CHIS VG 3 S12 33 30.7 E130 51 40.9 - DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS VG 4 S12 33 07.5 E130 51 38.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS VG 5 S12 32 41.2 E130 51 28.1 - DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ZT 1 S12 33 16.2 E130 52 32.1 S12 33 15.9 E130 52 30.4 DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ZT 2 S12 32 58.1 E130 52 30.1 S12 32 55.7 E130 52 31.1 DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ZT 3 S12 33 13.1 E130 51 22.7 S12 33 08.9 E130 51 25.8 DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ZT 4 S12 32 55.5 E130 51 32.5 S12 32 54.7 E130 51 31.2 DISCARDED - - - Golder Associates - - - - - - -DRW 5/08/2012 19 Channel Island CHIS ZT 5 S12 32 24.5 E130 52 11.2 S12 32 23.3 E130 52 09.2 DISCARDED - - - Golder Associates - - - - - - -DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 1 - - DRW1208FHWFC__02 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 1 - - DRW1208FHWFC__02 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 1 - - DRW1208FHWFC__02 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 2 - - DRW1208FHWFC__03 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 2 - - DRW1208FHWFC__03 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 2 - - DRW1208FHWFC__03 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 2 - - DRW1208FHWFC__03 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 2 - - DRW1208FHWFC__03 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno 0 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 3 - - DRW1208FHWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno - Brown spore UOT 0 0 Dr Gustaaf Hallegrae-DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF C_ 3 - - DRW1208FHWFC__01 Dinoflagellate - 1% Lugol's solution Golder Associates/Cardno (cf.avellana) 0 UOT 0 0 Dr Gustaaf Hallegrae0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_02 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MAGNT 41135 41135 Dr Chris Glasby TerebellidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno sandvichensis Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno cucullata Black-lipped rock oysMAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_03 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno sublaevigatum Trapezoidal clam MAGNT 41135 41135 Dr Richard Willan TrapezidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 1 S12 28 16.9 E130 50 48.5 - DRW1208FHWFDV_08 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno tintinnabulum 0 MITS 0 0 Rick Johnson 0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_04 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno dorsiporosa 0 MITS 0 0 Dr Dennis Gordon PetraliellidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_04 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno neritina 0 MITS 0 0 Dr Rad Nair 0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_04 Bryozoa 10% seawater buffered for 70% ethanol Golder Associates/Cardno lata 0 MITS 0 0 Dr Rad Nair 0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_07 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno vulsella Sponge-finger oyster MAGNT 41135 41135 Dr Richard Willan MalleidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_09 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno rosa 0 MITS 0 0 Rick Johnson 0DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno parvum 0 MITS 0 0 Mike Page PolyclinidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_05 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno avellana Ark clam MAGNT 41135 41135 Dr Richard Willan ArcidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_10 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno sandvichensis Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 2 S12 28 11.9 E130 50 57.9 - DRW1208FHWFDV_10 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno nucleus Knife oyster MAGNT 41135 41135 Dr Richard Willan IsognomonidaeDRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 3 S12 28 21.0 E130 50 42.3 - DRW1208FHWFDV_11 Polychaete 10% seawater buffered for - Golder Associates/Cardno stellatus Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 3 S12 28 21.0 E130 50 42.3 - DRW1208FHWFDV_11 Polychaete 10% seawater buffered for - Golder Associates/Cardno jukesii Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 3 S12 28 21.0 E130 50 42.3 - DRW1208FHWFDV_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno maritimus Scorched mussel MAGNT 41135 41135 Dr Richard Willan MytilidaeDRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 3 S12 28 21.0 E130 50 42.3 - DRW1208FHWFDV_14 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno tintinnabulum 0 MITS 0 0 Rick Johnson 0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 3 S12 28 21.0 E130 50 42.3 - DRW1208FHWFDV_15 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno 0 0 MITS 0 0 Rick Johnson 0DRW 13/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF DV 4 S12 28 19.0 E130 50 47.8 - DRW1208FHWFDV_13 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno cf. granulatum 0 MITS 0 0 Mike Page DidemnidaeDRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ED 1 S12 28 22.1 E130 50 44.6 S12 28 22.2 E130 50 46.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ED 2 S12 28 18.4 E130 50 52.8 S12 28 18.9 E130 50 51.1 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ED 3 S12 28 13.7 E130 50 51.2 S12 28 14.5 E130 50 48.1 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ED 4 S12 28 10.7 E130 50 56.9 S12 28 11.1 E130 50 56.8 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ED 5 S12 28 18.1 E130 50 58.5 S12 28 19.1 E130 50 53.0 DRW1208FHWFED_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates australis Kitten's paw clam MAGNT 41135 41135 Dr Richard Willan PlicatulidaeDRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 1 S12 28 19.9 E130 50 45.8 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 2 S12 28 15.8 E130 50 52.8 - DISCARDED - - - Golder Associates - - - - - - -

Location code

Collection date

Sublocation number


Sample method

Sample number


Remarks

DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 3 S12 28 14.4 E130 50 47.9 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 4 S12 28 06.7 E130 50 56.9 - DRW1208FHWFVG_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates subquadrata Diplodon clam MAGNT 41135 41135 Dr Richard Willan UngulinidaeDRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 4 S12 28 06.7 E130 50 56.9 - DRW1208FHWFVG_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates lochi Biscuit clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 4 S12 28 06.7 E130 50 56.9 - DRW1208FHWFVG_02 Green algae 10% seawater buffered for 70% ethanol Golder Associates racemosa var. lamou0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF VG 5 S12 28 09.8 E130 51 00.0 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ZT 1 S12 28 20.5 E130 50 46.2 S12 28 20.9 E130 50 43.7 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ZT 2 S12 28 17.2 E130 50 57.8 S12 28 17.4 E130 50 59.2 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ZT 3 S12 28 14.9 E130 50 47.0 S12 28 14.6 E130 50 48.5 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ZT 4 S12 28 07.0 E130 50 58.5 S12 28 07.9 E130 50 56.2 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF ZT 5 S12 28 09.3 E130 51 03.5 S12 28 08.0 E130 51 03.5 DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__01 Red algae 10% seawater buffered for 70% ethanol Golder Associates filamentosa 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__02 Red algae 10% seawater buffered for 70% ethanol Golder Associates flaccidum 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__02 Red algae 10% seawater buffered for 70% ethanol Golder Associates pannosa 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__03 Green algae 10% seawater buffered for 70% ethanol Golder Associates racemosa var. lamou0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__03 Green algae 10% seawater buffered for 70% ethanol Golder Associates verticillata 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__03 Green algae 10% seawater buffered for 70% ethanol Golder Associates flaccidum 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DRW1208FHWFP__04 Green algae 10% seawater buffered for 70% ethanol Golder Associates lentillifera 0 RBGS 41136 0 Prof Alan Millar 0DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 1 S12 28 05.8 E130 51 01.2 - DISCARDED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF P_ 2 S12 28 11.4 E130 50 55.5 - DISCARDED - - - Golder Associates - - - - - - -DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF BW 1 S12 28 17.8 E130 50 38.3 - NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 12/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF BW 2 S12 28 07.6 E130 50 54.8 - NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF T_ 1 S12 28 05.8 E130 51 01.5 - NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF T_ 2 S12 28 05.1 E130 51 02.2 - NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 7/08/2012 20 Fort Hill & Stokes Hill Wharves FHWF T_ 3 S12 28 04.8 E130 51 02.5 - NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 1 S12 27 40.8 E130 49 20.6 S12 27 41.4 E130 49 22.4 DISCARDED - - - Golder Associates - - - - - - -DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 2 S12 27 39.3 E130 49 16.3 S12 27 40.4 E130 49 19.3 DISCARDED - - - Golder Associates - - - - - - -DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_07 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates lochi Biscuit clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates australis True cockle MAGNT 41135 41135 Dr Richard Willan CardiidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 3 S12 27 38.1 E130 49 16.2 S12 27 39.6 E130 49 21.5 DRW1208HMASED_12 Bivalve 10% seawater buffered for 70% ethanol Golder Associates fragilis Theora clam MAGNT 41135 41135 Dr Richard Willan SemelidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_05 Algae 10% seawater buffered for 70% ethanol Golder Associates clavulatum 0 RBGS 41136 0 Prof Alan Millar 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_05 Algae 10% seawater buffered for 70% ethanol Golder Associates flaccidum 0 RBGS 41136 0 Prof Alan Millar 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_05 Algae 10% seawater buffered for 70% ethanol Golder Associates parvula 0 RBGS 41136 0 Prof Alan Millar 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_05 Algae 10% seawater buffered for 70% ethanol Golder Associates iyengarii 0 RBGS 41136 0 Prof Alan Millar 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_05 Algae 10% seawater buffered for 70% ethanol Golder Associates pannosa 0 RBGS 41136 0 Prof Alan Millar 0DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 4 S12 27 38.9 E130 49 20.9 S12 27 40.4 E130 49 24.2 DRW1208HMASED_06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates fortisulcata Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 5 S12 27 39.7 E130 49 23.2 S12 27 41.9 E130 49 26.8 DRW1208HMASED_13 Bivalve Fresh - Golder Associates japonicum Bag mussel MAGNT 41134 41134 Dr Richard Willan MytilidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 5 S12 27 39.7 E130 49 23.2 S12 27 41.9 E130 49 26.8 DRW1208HMASED_13 Bivalve Fresh - Golder Associates lochi Biscuit clam MAGNT 41134 41134 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 5 S12 27 39.7 E130 49 23.2 S12 27 41.9 E130 49 26.8 DRW1208HMASED_13 Bivalve Fresh - Golder Associates fragilis Theora clam MAGNT 41134 41134 Dr Richard Willan SemelidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_02 Fish 10% seawater buffered for 70% ethanol Golder Associates microcephalus Comb goby MAGNT 41135 41135 Dr Michael Hammer GobiidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates lochi Biscuit clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates donaciformis Wafer clam MAGNT 41135 41135 Dr Richard Willan TellinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cumingi Nut clam MAGNT 41135 41135 Dr Richard Willan NuculidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 6 S12 27 37.1 E130 49 19.4 S12 27 37.7 E130 49 19.6 DRW1208HMASED_11 Bivalve 10% seawater buffered for 70% ethanol Golder Associates fragilis Theora clam MAGNT 41135 41135 Dr Richard Willan SemelidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DISCARDED Hydroid 10% seawater buffered for 70% ethanol Golder Associates - - RBGS 41136 0 Prof Alan Millar -DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_03 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates - - MITS 0 0 Mike Page -DRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates rufescens Ark clam MAGNT 41135 41135 Dr Richard Willan ArcidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates lochi Biscuit clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates sp. 1 Diplodon clam MAGNT 41135 41135 Dr Richard Willan UngulinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates australis True cockle MAGNT 41135 41135 Dr Richard Willan CardiidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cumingi Nut clam MAGNT 41135 41135 Dr Richard Willan NuculidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 7 S12 27 37.9 E130 49 20.9 S12 27 38.9 E130 49 23.8 DRW1208HMASED_09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates semirugata Venus clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 8 S12 27 38.9 E130 49 23.9 S12 27 40.6 E130 49 26.9 DRW1208HMASED_08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 8 S12 27 38.9 E130 49 23.9 S12 27 40.6 E130 49 26.9 DRW1208HMASED_08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates lochi Biscuit clam MAGNT 41135 41135 Dr Richard Willan VeneridaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ED 8 S12 27 38.9 E130 49 23.9 S12 27 40.6 E130 49 26.9 DRW1208HMASED_10 Fish 10% seawater buffered for 70% ethanol Golder Associates rubristriatus Red eelgoby MAGNT 41135 41135 Dr Michael Hammer GobiidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 1 S12 27 40.8 E130 49 19.1 - DRW1208HMASVG_01 Bivalve 10% seawater buffered for 70% ethanol Golder Associates maritimus Scorched mussel MAGNT 41135 41135 Dr Richard Willan MytilidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 1 S12 27 40.8 E130 49 19.1 - DRW1208HMASVG_07 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates - - MITS 0 0 Mike Page -DRW 11/08/2012 21 HMAS Coonawarra HMAS VG 2 S12 27 39.0 E130 49 16.4 - DRW1208HMASVG_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates subquadrata Diplodon clam MAGNT 41135 41135 Dr Richard Willan UngulinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 2 S12 27 39.0 E130 49 16.4 - DRW1208HMASVG_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 21 HMAS Coonawarra HMAS VG 3 S12 27 36.8 E130 49 19.2 - DRW1208HMASVG_04 Polychaete 10% seawater buffered for 70% ethanol Golder Associates 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 3 S12 27 36.8 E130 49 19.2 - DRW1208HMASVG_05 Fish 10% seawater buffered for 70% ethanol Golder Associates albimaculosus Cream-spotted cardinMAGNT 41135 41135 Dr Michael Hammer ApogoninaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 3 S12 27 36.8 E130 49 19.2 - DRW1208HMASVG_08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS VG 4 S12 27 37.9 E130 49 20.6 - DRW1208HMASVG_06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates eucosmia Bittersweet clam MAGNT 41135 41135 Dr Richard Willan LucinidaeDRW 11/08/2012 21 HMAS Coonawarra HMAS ZT 1 S12 27 40.3 E130 49 25.3 S12 27 38.8 E130 49 22.4 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 21 HMAS Coonawarra HMAS ZT 2 S12 27 38.3 E130 49 18.8 S12 27 39.9 E130 49 21.8 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 21 HMAS Coonawarra HMAS ZT 3 S12 27 39.6 E130 49 18.2 S12 27 40.6 E130 49 21.8 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 1 S12 27 41.0 E130 51 01.4 S12 27 40.6 E130 51 05.2 DRW1208PKSHED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DISCARDED Polychaete 10% seawater buffered for - Golder Associates 0 ? Mollusc MAGNT 41135 41135 Dr Chris Glasby MolluscaDRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DRW1208PKSHED_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates crassa Basket clam MAGNT 41135 41135 Dr Richard Willan CorbulidaeDRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DRW1208PKSHED_04 Crab 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DRW1208PKSHED_06 Polychaete 10% seawater buffered for - Golder Associates 0 Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DRW1208PKSHED_06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates 0 0 MAGNT 41135 41135 Dr Richard Willan 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 2 S12 27 47.5 E130 51 07.5 S12 27 42.4 E130 51 06.3 DRW1208PKSHED_07 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 3 S12 27 36.6 E130 50 56.6 S12 27 36.1 E130 50 59.9 DRW1208PKSHED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ED 4 S12 27 34.9 E130 50 59.8 S12 27 35.3 E130 51 04.1 DRW1208PKSHED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates reticulatus 0 MITS 0 0 Rick Johnson 0DRW 11/08/2012 22 Perkins Shipping Yard PKSH ZT 1 S12 27 36.1 E130 50 58.0 S12 27 35.9 E130 50 59.7 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 22 Perkins Shipping Yard PKSH ZT 2 S12 27 41.3 E130 51 00.7 S12 27 40.7 E130 51 02.8 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 11/08/2012 22 Perkins Shipping Yard PKSH ZT 3 S12 27 39.7 E130 51 04.8 S12 27 36.1 E130 51 04.7 NO SAMPLE COLLECTED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ED 1 S12 30 10.2 E130 48 46.4 S12 30 05.7 E130 48 45.1 DISCARDED Ascidiacea - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ED 2 S12 29 57.4 E130 48 53.4 S12 29 51.5 E130 48 49.5 DRW1208IFV1ED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 ED 2 S12 29 57.4 E130 48 53.4 S12 29 51.5 E130 48 49.5 DRW1208IFV1ED_02 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 ED 3 S12 29 42.4 E130 48 39.3 S12 29 36.9 E130 48 37.2 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ED 4 S12 29 52.2 E130 48 37.9 S12 29 47.6 E130 48 36.1 DRW1208IFV1ED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 VG 1 S12 30 10.8 E130 48 46.6 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 VG 2 S12 29 40.6 E130 48 38.2 - DRW1208IFV1VG_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 VG 2 S12 30 00.4 E130 48 49.5 - DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 VG 4 S12 29 50.4 E130 48 37.4 - DRW1208IFV1VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 ZT 1 S12 30 11.0 E130 48 46.9 S12 30 08.3 E130 48 46.7 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ZT 2 S12 29 55.0 E130 48 48.2 S12 29 51.4 E130 48 47.0 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ZT 3 S12 29 40.1 E130 48 42.5 S12 29 36.3 E130 48 40.7 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 ZT 4 S12 29 52.5 E130 48 37.7 S12 29 49.2 E130 48 36.3 DISCARDED - - - Golder Associates - - - - - - -DRW 6/08/2012 23 IFFV1 IFV1 P_ 1 S12 30 11.4 E130 48 46.7 - DRW1208IFV1P__05 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates - - MITS 0 0 Mike Page -DRW 6/08/2012 23 IFFV1 IFV1 P_ 1 S12 30 11.4 E130 48 46.7 - DRW1208IFV1P__06 Bivalve 10% seawater buffered for 70% ethanol Golder Associates variegatus Barnacle - NOT A MOMAGNT 41135 41135 Dr Richard Willan BalanidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 1 S12 30 11.4 E130 48 46.7 - DRW1208IFV1P__07 Barnacles 10% seawater buffered for 70% ethanol Golder Associates amphitrite 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 P_ 2 S12 29 54.5 E130 48 47.6 - DRW1208IFV1ES_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates rosa 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 P_ 2 S12 29 54.5 E130 48 47.6 - DRW1208IFV1P__04 Crustacea 10% seawater buffered for 70% ethanol Golder Associates 0 0 MITS 0 0 Rick Johnson 0DRW 6/08/2012 23 IFFV1 IFV1 P_ 2 S12 29 54.5 E130 48 47.6 - DRW1208IFV1P__09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cucullina Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 2 S12 29 54.5 E130 48 47.6 - DRW1208IFV1P__09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates nucleus Knife oyster MAGNT 41135 41135 Dr Richard Willan IsognomonidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 2 S12 29 54.5 E130 48 47.6 - DRW1208IFV1P__09 Bivalve 10% seawater buffered for 70% ethanol Golder Associates sublaevigatum Trapezoidal clam MAGNT 41135 41135 Dr Richard Willan TrapezidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 3 S12 29 41.0 E130 48 38.4 - DRW1208IFV1P__08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates cucullina Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 3 S12 29 41.0 E130 48 38.4 - DRW1208IFV1P__08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates nucleus Knife oyster MAGNT 41135 41135 Dr Richard Willan IsognomonidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 3 S12 29 41.0 E130 48 38.4 - DRW1208IFV1P__08 Bivalve 10% seawater buffered for 70% ethanol Golder Associates pestigris Tiger's foot Oyster MAGNT 41135 41135 Dr Richard Willan OstreidaeDRW 6/08/2012 23 IFFV1 IFV1 P_ 3 S12 29 41.0 E130 48 38.4 - DRW1208IFV1P__11 Crustacea 10% seawater buffered for 70% ethanol Golder Associates reticulatus 0 MITS 0 0 Dr Shane Ahyong BalanidaeDRW 7/08/2012 24 IFFV2 IFV2 ED 1 S12 25 00.2 E130 47 42.1 S12 24 55.9 E130 47 39.3 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ED 2 S12 24 55.0 E130 47 35.8 S12 24 51.8 E130 47 34.8 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ED 3 S12 25 04.8 E130 47 31.3 S12 25 01.5 E130 47 29.8 DRW1208IFV2ED_01 Ascidiacea 10% seawater buffered for 70% ethanol Golder Associates/Cardno deerratum 0 MITS 0 0 Mike Page HolozoidaeDRW 7/08/2012 24 IFFV2 IFV2 ED 3 S12 25 04.8 E130 47 31.3 S12 25 01.5 E130 47 29.8 DRW1208IFV2ED_02 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno rosa 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 24 IFFV2 IFV2 ED 3 S12 25 04.8 E130 47 31.3 S12 25 01.5 E130 47 29.8 DRW1208IFV2ED_03 Polychaete 10% seawater buffered for 70% ethanol Golder Associates/Cardno stellatus Polychaete MAGNT 41135 41135 Dr Chris Glasby SerpulidaeDRW 7/08/2012 24 IFFV2 IFV2 ED 4 S12 25 12.7 E130 47 39.8 S12 25 07.0 E130 47 35.6 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ED 5 S12 25 14.9 E130 47 48.9 S12 25 07.8 E130 47 43.4 DRW1208IFV2ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 24 IFFV2 IFV2 ED 6 S12 25 06.4 E130 47 56.9 S12 25 01.5 E130 47 54.2 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ZT 1 S12 25 00.2 E130 47 42.1 S12 24 58.8 E130 47 41.1 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ZT 2 S12 25 04.8 E130 47 31.3 S12 25 03.0 E130 47 30.8 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ZT 3 S12 25 12.7 E130 47 39.8 S12 25 10.8 E130 47 38.9 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 24 IFFV2 IFV2 ZT 4 S12 25 06.4 E130 47 57.0 S12 25 04.3 E130 47 55.8 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 25 IFFV3 IFV3 ED 1 S12 29 44.0 E130 48 44.8 S12 29 44.3 E130 48 47.7 DRW1208IFV3ED_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ED 2 S12 29 52.2 E130 48 49.9 S12 29 57.1 E130 48 51.1 DRW1208IFV3ED_05 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ED 3 S12 29 37.6 E130 48 51.8 S12 29 40.5 E130 48 54.0 DRW1208IFV3ED_04 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ED 4 S12 29 44.1 E130 48 41.0 S12 29 48.5 E130 48 40.7 DRW1208IFV3ED_02 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno aureomaculatus Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 7/08/2012 25 IFFV3 IFV3 ED 4 S12 29 44.1 E130 48 41.0 S12 29 48.5 E130 48 40.7 DRW1208IFV3ED_03 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ED 5 S12 29 43.3 E130 48 45.1 S12 29 47.5 E130 48 45.9 DRW1208IFV3ED_06 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ED 5 S12 29 43.3 E130 48 45.1 S12 29 47.5 E130 48 45.9 DRW1208IFV3ED_07 Bivalve 10% seawater buffered for 70% ethanol Golder Associates/Cardno aureomaculatus Fingernail clam MAGNT 41135 41135 Dr Richard Willan SolenidaeDRW 7/08/2012 25 IFFV3 IFV3 ED 6 S12 29 35.3 E130 48 35.1 S12 29 38.7 E130 48 37.9 DRW1208IFV3ED_08 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 VG 1 S12 29 46.6 E130 48 48.5 - DRW1208IFV3VG_01 Barnacles 10% seawater buffered for 70% ethanol Golder Associates/Cardno amphitrite 0 MITS 0 0 Rick Johnson 0DRW 7/08/2012 25 IFFV3 IFV3 ZT 1 S12 29 44.0 E130 48 44.8 S12 29 43.9 E130 48 45.7 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 25 IFFV3 IFV3 ZT 2 S12 29 52.2 E130 48 49.9 S12 29 53.4 E130 48 50.2 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 25 IFFV3 IFV3 ZT 3 S12 29 44.0 E130 48 41.0 S12 29 46.2 E130 48 41.1 DISCARDED - - - Golder Associates/Cardno - - - - - - -DRW 7/08/2012 25 IFFV3 IFV3 ZT 4 S12 29 35.3 E130 48 35.1 S12 29 36.5 E130 48 36.2 DISCARDED - - - Golder Associates/Cardno - - - - - - -




APPENDIX D RECORD OF ADDITIONAL DATA FROM PHOTOQUADRATS


Prepared for INPEX Cardno 55

Appendix D: Record of observations of potential marine pest species in photoquadrats and crab pots undertaken as part of the Coral, Seagrass,

Subtidal Benthos and Recreational Fishing Monitoring Programs

All CPCE Substrata ‘Coral’ detailed in the Coral Monitoring Program Baseline Report (Cardno 2012b)

CPCE Substrata

Unique Photograph Identifier

Provisional Pest Identification Number Observed

Data Recorder

Date Recorded

Outcome

Scientific Name Common Name

1 Coral MA_S1_T2_30 Caulerpa racemosa

Grape Algae Several BM 10/09/12 Request made for specimen collection



3 Coral CI_S1_T2_29 Hydroides dianthus/sanctaecrucis

Tube worm 1 BM 10/09/12 Morphology of operculum not consistent with pest species

4 Coral SS_S1_T3_42 Hydroides dianthus/sanctaecrucis

Tube worm 2 GC 10/09/12 Morphology of operculum not consistent with pest species

5 Coral SS_S1_T4_2,13,14,

15,18,19,20,21,40

Caulerpa racemosa

Grape Algae Several GC 10/09/12 Request made for specimen collection

6 Coral SS_S2_T2_49 Sabella spallanzanii

European fan worm

2 GC 10/09/12 Morphology of radiolar crown not consistent with pest species

7 Coral SS_S2_T3_3,15,42

Sabella spallanzanii

European fan worm

1-2 GC 10/09/12 Morphology of radiolar crown not consistent with pest species

8 Coral SS_S2_T4_2,14,15,

17,18,20,21,40

Caulerpa racemosa


9 Coral SS_S3_T3_10 Caulerpa racemosa


10 Coral SS_S3_T4_2,10,13,

14,15,17,18,20,21

Caulerpa racemosa




CPCE Substrata



Data Recorder

Date Recorded

Outcome


11 Coral SS_S3_T3_18 Sabella spallanzanii

European fan worm

1 GC 10/09/12 Morphology of radiolar crown not consistent with pest species





14 Coral MA_S3_T4_10 Hydroides dianthus/sanctaecrucis


15 Coral W2_S3_T1_14 Hydroides dianthus/sanctaecrucis


16 Coral WP_S2_T1_35 Caulerpa racemosa

Grape Algae 5 KM 11/09/12 Request made for specimen collection





19 Coral WP_S2_T2_4 Sabella spallanzanii

European fan worm

1 KM 11/09/12 Morphology of radiolar crown not consistent with pest species


European fan worm



European fan worm



European fan worm



European fan worm


24 Coral WP_S1_50 Caulerpa racemosa

Grape Algae 1 YC 11/09/12 Request made for specimen collection

25 Coral MA_S1_T1_20 Caulerpa Grape Algae Several AN 11/09/12 Request made for specimen collection



CPCE Substrata



Data Recorder

Date Recorded

Outcome


racemosa


Tube worm Several AN 11/09/12 Morphology of operculum not consistent with pest species


Tube worm 1 AN 11/09/12 Morphology of operculum not consistent with pest species



29 Coral CP_S3_T2_14 Hydroides dianthus/sanctaecrucis


30 Coral CP_S3_T4_10 Hydroides dianthus/sanctaecrucis






33 Coral WP_S3_T2_34,37

Hydroides dianthus/sanctaecrucis


34 Coral WP_S3_T3_04 Hydroides dianthus/sanctaecrucis




36 Coral W2_S2_T1_19 Hydroides dianthus/sanctaecr




CPCE Substrata



Data Recorder

Date Recorded

Outcome


ucis





39 Coral FS04_WR1_TR02_Q07









42 Coral FS04_WR1_T3_Q26



43 Coral FS04_WR1_T2_Q07,36



44 Coral FS04_WR2_T1_Q09,21









47 Coral FS04_CHI_T1_Q38

Hydroides dianthus/sanctaecr




CPCE Substrata



Data Recorder

Date Recorded

Outcome


ucis







all Seagrass All seagrass photoquadrats (Cardno 2012c)

No provisional identification of pest species

0 24-27/10/12

22-25/11/12

No further action required

all Subtidal Benthos (epifauna)

All Subtidal Epibenthos photoquadrats (Cardno 2012d)


0 10-17/10/12 No further action required

all Crab traps (Research angling records)

All crabs collected in crab pots in Recreational Fish and Fish Health Monitoring Program/Recreational Fishing /Baseline Phase (Cardno 2012e)


0 12-14/11/12 No further action required