↘ MALARIA AG ELISA

MALARIA AG ELISAREF: 650101

CE MARKED

QUALITATIVE ASSAY

SENSITIVITY: < 0.2 ng/ml pLDH

< 30 parasites/µl

INCUBATION TIME: 120’

AVAILABLE FORMAT: 192T

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EN ISO 13485: 2012 CERTIFIED COMPANY

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↘ MALARIA AG ELISAMalaria is a serious, sometimes fatal blood-born parasitic disease resulting from infection

with protozoa of the genus Plasmodium and is transmitted by the bite of Anopheles

mosquitoes. About half of the world’s population is at risk of malaria, leading to

250 million malaria cases yearly and nearly one million deaths. Four species infect

humans: P. falciparum, P. vivax, P. ovale and P. malariae.

Prompt and accurate diagnosis of malaria is needed for implementation of appropriate

treatment to reduce associated morbidity and mortality. The first symptoms (most often

fever, chills, sweats, headaches, muscle pains, nausea and vomiting) are not specific

and may resemble those of many other infectious diseases like the flu. In severe malaria,

clinical findings (confusion, coma, neurologic focal signs, severe anemia, respiratory

difficulties) are more striking and may increase the suspicion index for malaria. It is

essential to confirm the clinical diagnosis with results from the laboratory. Treatment

must start early to cure the disease effectively, which is also important in preventing the

spreading of the disease. Laboratory diagnosis of malaria is based on the identification

of the malaria parasite or its antigens in the blood of the patient. While microscopy is

considered as the gold standard, assays detecting the Plasmodium specific enzyme

lactate dehydrogenase (pLDH) are considered a prominent alternative. All four human

malarial parasites produce a unique pLDH enzyme while its presence follows the level

of parasitemia making it a good monitoring tool for following active malarial infections.

Even though malaria is a tropical disease, cases of malaria may appear all over the

world due to increased travelling practices. As the disease may also be transmitted by

blood transfusion, control of donor blood for the presence of the parasite is essential.

The apDia Malaria antigen test is a sandwich Enzyme-Linked ImmunoSorbent Assay

(ELISA) for the detection of the malarial antigen pLDH of any of the four species in blood

samples. It may also be used to measure in vitro drug susceptibility in culture samples.

The ELISA kits offered by apDia are validated on open ELISA automates such as the

Dynex Instruments. Raadsherenstraat 32300 Turnhout, BelgiumT +32 14 45 35 99F +32 14 81 29 45admin@apdia.bewww.apdiagroup.com

MA

LAG

/07-

2014