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LOCAL ORGANISING COMMITTEE Stephen A Renshaw, PhD Co-chair University of Sheffield, UK Sarah Walmsley, PhD Co-chair University of Edinburgh, UK Edwin R Chilvers, PhD Imperial College London, UK Alison Condliffe, PhD University of Sheffield, UK Janet Lord, PhD University of Birmingham, UK Sussan Nourshargh, PhD Queen Mary University London, UK Adriano Rossi, PhD University of Edinburgh, UK Sonja Vermeren, PhD University of Edinburgh, UK Helen L Wright, PhD University of Liverpool, UK NEUTROPHIL MEETINGS STEERING COMMITTEE Sylvain G Bourgoin, PhD Université Laval, Québec City, Canada Patrick P McDonald, PhD Université de Sherbrooke, Sherbrooke, Canada Patrice E Poubelle, MD Université Laval, Québec City, Canada Marc Pouliot, PhD Université Laval, Québec City, Canada Webmaster Patrick P McDonald, PhD

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INTERNATIONAL ADVISORY BOARD Lee-Ann H Allen, Iowa City, USA Nancy Berliner, Boston, USA Sylvain Bourgoin, Québec City, Canada Sven Brandau, Essen, Germany Marco A Cassatella, Verona, Italy Sergio Catz, La Jolla, USA Mary C Dinauer, St-Louis, USA Michael Glogauer, Toronto, Canada Sergio Grinstein, Toronto, Canada Anna Huttenlocher, Madison, USA Daniel Irimia, Boston, USA Friederike Jönsson, Paris, France Mariana Kaplan, Bethesda, USA Ralph Kettritz, Berlin, Germany Leo Koenderman, Utrecht, The Netherlands Paul Kubes, Calgary, Canada Klaus Ley, La Jolla, USA Erzsébet Ligeti, Budapest, Hungary Clifford A Lowell, San Francisco, USA Kenneth McLeish, Louisville, USA William M Nauseef, Iowa City, USA Mauro Peretti, London, UK Carlos Rosales, Mexico, Mexico Irina Udalova, Oxford, UK Silvia Uriarte, Louisville, USA Véronique Witko-Sarsat, Paris, France Teizo Yoshimura, Fredrick, USA Arturo Zychlinsky, Berlin, Germany MEETING PLATFORM The meeting will be delivered via Whova. Livestreamed sessions will be recorded to accommodate delegates across different time zones, as part of the registration. All times shown in the scientific program are in British Summer Time which is GMT+1. The meeting can be accessed via this link: https://whova.com/portal/neutr_202005 MEETING PLATFORM TECHNICAL TEAM Daniel Irimia, Harvard University Stephen Renshaw, University of Sheffield Felix Elliott, Harvard University Alex Hopke, Harvard University Regis Joulia, Queen Mary University London Loic Rolas, Queen Mary University London Maria Prendicki, Imperial College London Katherine Lodge, Imperial College London Michele Fresneda Alarcon, University of Liverpool Stuart Gaines, University of Sheffield

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SCIENTIFIC PROGRAM All times are shown in British Summer Time which is GMT+1 All sessions will be recorded and registration for the meeting will provide access to all recorded content. Monday May 31 11h00

Welcome Co-chairs (S Renshaw, S Walmsley)

11h15 Keynote Lecture: Mikala Egeblad (Cold Spring Harbor Laboratory USA) NETs in flammes: neutrophil extracellular traps in cancer and COVID-19

Monday May 31 Parallel Session 1 Session 1: Ongoing warfare: neutrophil-pathogen interactions and their outcomes Chair: Alison Condliffe 12h00-12h30 Bryan Yipp (University of Calgary, Canada)

A neutrophil cell atlas contextualises COVID-19 from sepsis

12h30-12h35 Flash talk #105: Drinalda Cela (University of Bristol, Bristol UK) PAD4 controls chemoattractant production and neutrophil trafficking in malaria

12h35-13h05 Alison Criss (University of Virginia, Charlottesville, USA) Gonococcal suppression of neutrophil antimicrobial activities

13h05-13h10 Flash talk #28: Nicole Sarden (University of Calgary, Alberta, Canada) Impaired neutrophil-mediated host defense during viral-associated aspergillosis

13h10-13h40 Isaac Chiu (Harvard University, Boston, USA) Neuronal regulation of innate immunity in bacterial host defence

13h40 Close session 13h40-14h30 BREAK

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Monday May 31 Parallel Session 2 Session 2: Neutrophil niches Chair: Helen Wright 12h00-12h30 Mia Phillipson (Uppsala University, Sweden)

On the origin and recruitment of pro-angiogenic neutrophils in ischaemic injury

12h30-12h35 Flash talk #10: Klara Soukup (University of Lausanne, Lausanne, Switzerland) Activation and phenotype of neutrophils in the brain tumour microenvironment

12h35-13h05 Filip Swirski (Mount Sinai, New York, USA) Lifestyle, cardiovascular disease, and the non-neutral neutrophil

13h05-13h10 Flash talk #16: Melissa Meyer (La Jolla Institute, La Jolla, USA) Discovery of a neutrophil subset that expresses B cell features and modulates CD4+ T cells in human melanoma

13h10-13h40 Anjelica Gonzalez (Yale University, New Haven, USA) Microenvironment influence on neutrophil activity

13h40 Close session 13h40-14h30

BREAK

Monday May 31 Plenary Session Session 3: Neutrophils in disease Chair: Janet Lord 14h30-15h00 Attila Mocsai (Semmelweiss University, Budapest, Hungary)

Neutrophil signalling in autoimmune and inflammatory disease models

15h00-15h05 Flash talk #108: Irem Oezel (University Hospital Essen, Essen, Germany) Neutrophil mediated regulation of B-cells in tumour draining lymph nodes of head and neck cancer patients

15h05-15h35 Suchita Nadkarni (Queen Mary University London, UK) Neutrophil and T-cell crosstalk in vascular diseases

15h35-15h40 Flash talk #112: Juma El-awaisi (University of Birmingham, Birmingham, UK) Age and gender specific changes in the neutrophil response to Myocardial Infarction – Intravital investigations in the injured beating coronary microcirculation

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15h40-16h10 Ilaria Malanchi (Francis Crick Institute, London, UK) The dark side of neutrophils healing power

16h10-16h15 16h15

Flash talk #163: Anna Shen (McGill University, Montreal, Canada) Investigating the impact of neutrophils on natural killer cell activity in breast cancer liver metastasis Close session and close Day 1

Tuesday June 1 11h00

Welcome Co-chairs (S Renshaw, S Walmsley)

Tuesday June 1 Parallel Session 1 Session 4: Resolution and repair Chair: Adriano Rossi 11h10-11h40 Trian Chavakis (Technische Universität, Dresden, Germany)

Del-1 in resolution of inflammation

11h40-11h45 Flash talk #79: Meriem Sekheri (University of Montreal, Montreal, Canada) Aspirin-triggered pro-resolving lipids restore impaired neutrophil phagocytosis to promote resolution of bacterial infections

11h45-12h15 Lucy Norling (Queen Mary University, London, UK) Neutrophil-derived extracellular vesicles promote resolution of arthritis

12h15-12h20 Flash talk #51: Heidi Welch (Barbraham Institute, Cambridge, UK) The GPCR adaptor protein norbin suppresses the neutrophil-mediated immunity of mice to Pneumococcal infection

12h20-12h50 Bruce Levy (Harvard University, Boston, USA) A surprising mechanism for neutrophil cytoplasts in non-T2 inflammation in asthma and their regulation by SPM

12h50-12h55 Flash talk #97: Annika Heuer (University Medical Campus Hamburg-Eppendorf, Hamburg, Germany) Therapeutic targeting of neutrophil extracellular traps improves primary and secondary intention wound healing in mice

12h55 Close session 12h55-14h00

BREAK

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Tuesday June 1 Parallel Session 2 Session 5: Neutrophil trafficking and signalling Chairs: Sussan Nourshargh and Sonja Vermeren Session sponsor: Biochemical Society 11h10-11h40 Graham Lieschke (Royal Melbourne Hospital, Melbourne, Australia)

Neutrophils at work! New insights from in vivo imaging in zebrafish

11h40-11h45 Flash talk #59: Loic Rolas (Queen Mary University, London, UK) Inflamed aged tissues support enhanced neutrophil reverse transendothelial cell migration: A crucial role for mast cells

11h45-12h15 Barbara Walzog (Ludwig-Maximillians Universität, Munich, Germany) Integrin signalling and neutrophil trafficking in innate immunity

12h15-12h20 Flash talk #24: Borja Prat-Luri (University of Lausanne, Lausanne, Switzerland) Neutrophil trafficking to draining lymph nodes in response to Leishmania mexicana

12h20-12h50 Jamel El-Benna (Université Diderot, Paris, France) Signalling pathways involved in p47phox phosphorylation and NADPH oxidase priming and activation

12h50-12h55 Flash talk #46: Evelien Sprenkeler (Sanquin/Amsterdam UMC, Amsterdam, Netherlands) Defective neutrophil transendothelial migration and lateral motility in ARPC1B deficiency under flow conditions

12h55 Close session 12h55-14h00

BREAK

Tuesday June 1 Plenary Session Session 6: Neutrophil kinetics and fate Chair: Edwin Chilvers 14h00-14h30 Sara Rankin (Imperial College, London, UK)

Neutrophil redistribution following plerixafor or infection

14h30-14h35 Flash talk #3: Ben Wielockx (Technische Universität Dresden, Dresden, Germany) HIF2a is a direct regulator of neutrophil motility

14h35-15h05 Lai Guan Ng (A*STAR, Singapore) Neutrophils: the power of many

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15h05-15h10 Flash talk #76: Connie Shen (McGill University, Montreal, Canada) Neutrophil nuclear segmentation facilitates migration through tight constrictions

15h10-15h40 Andrés Hidalgo (Spanish National Centre for Cardiovascular Research, Madrid, Spain) The diversity of neutrophils in tissues

15h40-15h45 Flash talk #93: Alex Hopke (Massachusetts General Hospital, Boston, USA) Large scale arrays to study neutrophil swarming against fungi

15h45 Close session 15h45-16h00

BREAK

Tuesday June 1 Plenary Session 16h00-16h45

Keynote Lecture: Tanya Mayadas (Harvard University, Boston, USA) Harnessing neutrophil plasticity for the treatment of disease

16h45-17h00 Break

17h00-17h30 Patrick McDonald (Université de Sherbrooke, Sherbrooke, Canada) Community meeting, including news from Neutrophil Core Group about future Neutrophil meetings

17h30-18h00 Organisers’ comments and close

18h00

Meeting end