LIFE10 ENV/IT/000364 «ECOFATTING»«Environmentally friendly natural products

instead of cloroparaffines in the fatting phase of the tanning cycle»

EMILIA BRAMANTIICCOM-PISA, CNR

Coordinator

Final Meeting, ICCOM CNR Pisa December11th 2013

AGENDA

9,45: Welcome to the partecipants and beneficiaries’ presentation (roles in the project)

10.00: technical session

-project overview: objective, actions, state of progress (ICCOMCNR)-Action by action description of the activities implemented (including dissemination)-Results achieved, future developments and possible critical issues

Financial session (parallel to technical session)

-Short introduction, state of project expenditures (by Coordinating Beneficiary)-Check of the accounting system, management and financial documentation-Discussion with European Commission and External Monitoring Team

13.00 Break

Summary of the Project

PROJECT’S TARGETReplacing chlorinated hydrocarbons (CPs and SCPs) used in leather manufacturing as lubricating and tanning agents

Drawbacks:-high chlorine content-low biodegradability

The GREEN ALTERNATIVE PRODUCTS:

A set of derivatives from natural products based on Fatty Acid Methyl Ester (FAME-Cl) were selected as best candidates. Selection criteria was based on the carbon chain lenght (C12–C18) and the chlorine content

INNOVATIVE EFFECTS-Increased penetration of fat in derma, thus resulting more effective products- better performances-less wastewater contamination-higher biodegradability-High aesthetical profile (touch and fullnessI)

Actions carried out by each partner

ACTIONS

fat agentsenvironmental impact

natural productslaboratory level

semi-industrial levelpre-industrial level

environmental demoenviron benefits

quality assessmenttecnico-econ viability

ManualLeather sample book

material for workshopsdiffusion mat prep

Italian trainingSpanish training

Italy workshopSpain workshop fairs

Intern fairs /other eventsdigital supports

international disseminationAfter-LIFE Comm Plan

Project managementMonitoring

audit

COLORTEX, ICCOM, SERICHIM, ENEA

INESCOP............

COLORTEX, ICCOM, SERICHIM, ENEA

COLORTEX, SERICHIM, INESCOP

COLORTEX, SERICHIM INESCOP

COLORTEX, ICCOM, SERICHIM, ENEA, INESCOP

ICCOM, ENEA, INESCOP

ICCOM, ENEA, INESCOP

COLORTEX, SERICHIM, INESCOP

ENEA ..........

COLORTEX, INESCOP

COLORTEX, INESCOP

COLORTEX, ICCOM, SERICHIM, ENEA, INESCOP

COLORTEX, ICCOM, SERICHIM, ENEA, INESCOP

COLORTEX.........

INESCOP...........

COLORTEX...........

INESCOP...........

COLORTEX, ICCOM, SERICHIM, ENEA, INESCOP

ENEA...........

COLORTEX, ICCOM, SERICHIM, ENEA, INESCOP

ENEA...........

ICCOM...........

ICCOM...........

ICCOM...........

Activity reports

Kick off meeting on January 18th 2012 (COLORTEX, Italy) 6th month meeting on June 2012 (ICCOM CNR, Italy) and 1st monitoring

team visit Inception Report submitted on September 2012 12th month meeting on January 2013 (SERICHIM, Italy) Progress Report submitted on May 2013 Deliverables submitted (Action 1-2; 3; 4; 5 and 14) 18th month meeting on June 2013 (INESCOP, Spain) 2nd Monitoring Team visit on June 25th 2013 (ICCOMCNR) 24th month meeting on December 11th 2013 (ICCOM CNR)

NEXT COMMITMENTS: Final meeting (December 2013, ICCOMCNR) Deliverables of Actions 6, 7-8, 10, 20, 14/ 21, 13-22, 20, 23) Final Report with payment request (January 2014) Audit (Deliverable Action 25)

ICCOM CNR activities 18-24 months

DISSEMINATION

ACTIONS 6, 7, 9

- Quality assessment of leather and products by GC-MS and FTIR

Networking and continuation and valorization of the project

PODEBA Life10 ENV/IT/365 « Use of poultry dejection in the bating fase in the tanning cycle”

LIFE12 ENV/IT/000352 “BIONAD «Naturalised dyes replacing commercial colorants for environmentally friendly leather dyeing and water recycle”

Life 20??: the LIFE of LIFEs is in the air...

GAS CHROMATOGRAPHY-MASS SPECTROMETRIC ANALYSIS OF LEATHER AND PRODUCTS TREATED FOLLOWING THE AMEK PROCEDURE (PODEBA) AND FATTED WITH ECOFATTING PRODUCTS.

Injection Volume 1000 L

Incubation Temperature and time 60°C for 1800 s

Syringe Temperature 65 °C

Injector Split: 10:1

Temperature 200°C

Column DB624 60 m 0.25 mm i.d. 1.4 mm df

Gas Flow 1.0 ml/min He

Chromatographic conditions 30 °C for 5 min up to 240 °C (10.00 °C/min) in 10.00 min

Detector MS FULL_SCAN 10-350 M/z EI 70 eV MS temp 230°C

Head space CGMS analysis of fresh and treated poultry in order to evidence the main compounds responsible for the bad smell and the effect of PODEBA treatment. One g of each poultry sample was placed in a 10 mL vial.

Head space GCMS method for poultry, leather and products treated with PODEBA procedure.

HS-GCMS system CG6850 coupled to MS 5975C MSD and HS autosampler CombiPal CTC-80 (Agilent Technologies, Milan, Italy).

Representative head space GCMS chromatogram of the poultry samples before (PF1) and after PODEBA treatment (p0-1, c1-1, amekG-1, amek8-1).

RT: 4.53 - 23.54

5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23Time (min)

0

50

1000

50

1000

50

100

Re

lativ

e A

bu

nd

an

ce

0

50

1000

50

1002-Butanolo

MeOHEtOH

DMS

AcOMe 2-Pentanolo

5.81

13.05 15.28 17.3714.62 16.9212.534.96 19.8711.669.64 17.46 22.9322.2520.74 21.237.30 19.608.62

2-ButanoneAldeide C4

Pentano

Esanale

Pentanale

5.81

19.82

17.637.01

16.9214.62 20.26 22.07 22.2621.238.94 10.45 19.5718.328.484.87 7.21

2-Pentanone

5.81

8.64 19.826.54 21.7720.4417.6316.82 22.4014.789.648.474.86

13.0511.66 22.9419.1915.9111.81 14.027.24 10.9510.19

5.81

22.0718.26 20.62 22.9321.226.36 16.92 20.268.645.02 10.47 17.64 19.5615.757.20 14.6313.06 13.6110.967.53 12.229.00 9.64

5.83

22.886.45 21.2210.45 22.2120.265.08 18.25 19.6717.58 19.257.20 17.2615.9213.0610.94 15.587.51 13.61 14.618.63 12.5110.21 11.989.21

NL:1.00E7TIC MS PF-1

NL:1.00E7TIC MS p0-1

NL:1.00E7TIC MS c1-1

NL:1.00E7TIC MS amek-6-1

NL:1.00E7TIC MS amek-8-1

x10 x10 x10

It is worth noting that after the complete PODEBA treatment no volatile compounds were found.

HS-GCMS analysis and FTIR analysis confirm that leathers and products, and waste waters have no bad-smelling related to PODEBA treatment .

CONCLUSIONS (I): ECOFATTING natural products can be good subsitutes of CPs (fattening)

0 10 20 30 40 50

0,0

0,5

1,0

1,5

2,0

Pea

k he

ight

173

7 cm

-1

CMS, Cl %

gelatin/CMSs

CP

30

CP

44

CM

S38

FA

ME

Cl-

FA

ME

(4

0,5

%C

l)

Cl-

FA

(51

,7%

Cl)

PO

-FA

ME

Cl-

PO

-FA

ME

(48.

6% C

l)

0

2

4

a.u.

The interaction still depends on the chloride content. However, natural products are more effective (less amount required) and more biodegradable

CONCLUSIONS (II): natural products can replace SCPs (tanning and fattening)

20%w/w, 30°C 70% w/w, 30°C 20%w/w, 60°C 70% w/w, 60°C0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

peak

are

a at

115

9 cm

-1

GSA/SCP

20%w/w, 30°C 70% w/w, 30°C 20%w/w, 60°C 70% w/w, 60°C0.0

0.2

0.4

Pea

k ar

ea a

t 173

5 cm

-1

GSA/SCMS

20%w/w, 30°C 70% w/w, 30°C 20%w/w, 60°C 70% w/w, 60°C0

2

4

a.u.

GSA/SCPO-FAME

20%

w/w, 3

0°C

70%

w/w

, 30°

C

20%

w/w, 6

0°C

70%

w/w

, 60°

C

0.0

0.2

0.4

0.6

0.8

1.0

1.2

Pe

ak

are

a a

t 17

35

cm

-1 CL/SCMS

20%

w/w, 3

0°C

70%

w/w

, 30°

C

20%

w/w, 6

0°C

70%

w/w

, 60°

C0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0CL/SCPO-FAME

a.u.

20%

w/w, 3

0°C

70%

w/w

, 30°

C

20%

w/w, 6

0°C

70%

w/w

, 60°

C

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

p

eak

area

at

115

9 cm

-1

CL/SCP

Conventional products SCMS SC-FAME (Palkernel oil)

Gelatin/SCPGelatin/SCMS

Gelatin/SCFAME

CL/SCP CL/SCMS

CL/SCFAME

CONCLUSIONS (III)

•Short-chain SCPs were located prevalently between the collagen fibrils •long-chain SCPs had the tendency to wrap around more triple helical•SCPs interact with the side chains of positively charged amino acids, namely Arg and Lys, and of those amino acids containing an amine group, namely Asn, Gln, suggesting the formation of covalent thionylamine bonds. •Strong hydrogen bonding interactions were also engaged between the SCP oxygens and the hydroxyl groups of Hyp, Ser and Thr.

ECOFATTING PEOPLE @ ICCOM-PI

Emilia BramantiAlessandro D’Ulivo

Massimo Onor

Manuela CempiniValentina Della PortaSusanna Monti