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Research Articles: Development/Plasticity/Repair
Lhx2 interacts with the NuRD complex and regulates cortical neuron subtypedeterminants Fezf2 and Sox11
Bhavana Muralidharan1, Zeba Khatri1, Upasana Maheshwari1, Ritika Gupta1, Basabdatta Roy1, Saurabh J.
Pradhan2, Krishanpal Karmodiya2, Hari Padmanabhan1,3,4, Ashwin Shetty1,3, Chinthapalli Balaji1, Ullas Kolthur-
Seetharam1, Jeffrey D. Macklis3,4, Sanjeev Galande2 and Shubha Tole1
1Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai-400005, India2Indian Institute of Science, Education, and Research, Pune- 411008, India.3Department of Stem Cell and Regenerative Biology4Center for Brain Science, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA-02138 USA.
DOI: 10.1523/JNEUROSCI.2836-16.2016
Received: 5 September 2016
Revised: 26 October 2016
Accepted: 7 November 2016
Published: 1 December 2016
Author contributions: B.M., Z.K., U.M., S.J.P., H.P., A.S.S., U.K.-S., S.G., and S.T. designed research; B.M.,Z.K., U.M., R.G., B.R., S.J.P., H.P., A.S.S., and C.B. performed research; B.M., Z.K., U.M., R.G., B.R., K.K.,H.P., A.S.S., U.K.-S., J.D.M., S.G., and S.T. analyzed data; B.M., Z.K., H.P., A.S.S., J.D.M., and S.T. wrote thepaper.
Conflict of Interest: The authors declare no competing financial interests
We thank Y. Li and E. Monuki for kind gifts of mouse lines used in this study; T. Saito, T. Ohshima, L. Carlsson,E. Monuki, S. McConnell, A. Stoykova, C. Ragsdale and R. Hevner for gifts of plasmid DNA. We thank P.Arlotta, D. Jabaudon, S. Lodato and members of the Tole lab for helpful discussion, D. Jabaudon for criticalcomments on the manuscript, Dr. S. Suryavanshi and the TIFR Animal Facility for excellent support, and L.D'souza for technical assistance. This work was supported by a Wellcome Trust-Department of BiotechnologyIndia Alliance Early Career Fellowship (BM), and intramural funds from TIFR-DAE (ST).
Corresponding author: [email protected]
Cite as: J. Neurosci 2016; 10.1523/JNEUROSCI.2836-16.2016
Alerts: Sign up at www.jneurosci.org/cgi/alerts to receive customized email alerts when the fully formattedversion of this article is published.
Fezf2 Sox11
1Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai-
400005, India; 2Indian Institute of Science, Education, and Research, Pune- ,
India. 3Department of Stem Cell and Regenerative Biology, and 4Center for Brain
Science, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA-
USA.
* Corresponding author: [email protected]
Current affiliation: Department of Stem Cell and Regenerative Biology, and 4Center for
Brain Science, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA-
02138 USA
Fezf2 Sox11
Fezf2 Sox11
Fezf2
Ctip2
Fezf2 Sox11
Fezf2
in vivo
Fezf2
Fezf2
Fezf2 Sox11 that
Lhx2
Lhx2
Lhx2
Fezf2
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2
Fezf2
et al
β
In situ
Lhx2
Lhx2
Lhx2
Lhx2
Lhx2
Lhx2
Tbr1
Lhx2
ER81 Id2, Fezf2
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Lhx2
Fezf2
Lhx2cKO Sox11,
Lhx2cKO
Fezf2
Fezf2/Sox11
Fezf2 Sox11
Lhx2
Fezf2
Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2
Sox11
Fezf2 Sox11
Lhx2cKO
Fezf2 Sox11
Lhx2cKO
Fezf2 Sox11
Fezf2
Lhx2
Lhx2-GFP in utero
Lhx2
Fezf2
Fezf2
Fezf2 Sox11
Fezf2 Sox11
Fezf2
Lhx2
Lhx2 Lhx2
Lhx2 Fezf2
Lhx2
Lhx2
Lhx2
Lhx2
Lhx2
Fezf2 COUP-TF1, CB1R and Cux2
Fezf2
Fezf2
Fezf2
Ctip2
Ctip2
Fezf2 Fezf2
Fezf2
Fezf2
Lhx2
Lhx2cKO
Tbr1 Fezf2,
ER81, and Id2
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
Fezf2 Sox11
The image of the silver stained gel shown in (A) has been cropped to remove the lanes
corresponding “unbound” fractions of control IgG and Lhx2 IP that were on the left of the
marker lane. The Western blots shown in (B) and (C) are cropped from full length
Western blots. The original uncropped images are available upon request.
Fezf2 Sox11
Lhx2cKO
Fezf2 Sox11
GFP Lhx2-GFP
Lhx2-GFP
Lhx2
