Lecture 19-20Atomic Spectroscopy in Analytical Chemistry

Reading: Ch. 21

Outline

1. Introduction (21-1).

2. Methods of atomization (21-2)

3. Effect of temperature on excited state population (21-3).

4. Instrumentation: Atomic absorption (21-4).

Atomic Spectroscopy

1. Atomic absorbance

2. Atomic emission

3. Atomic fluorescence

Atomic Spectroscopy

Atomization: a Flame

Atomization: a Flame

Atomization: a Furnace

Atomization: Inductively Coupled Plasma (ISP)

Atomization: Inductively Coupled Plasma (ISP)

ktEegg

NN /

0

Δ−∗∗

=

Outline

1. Instrumentation: Atomic emission (21-1).

2. Instrumentation: Atomic absorption (21-1).

3. Instrumentation: Atomic fluorescence (21-1).

4. Instrumentation: ICP-MS (21-2).

5. Noise and low detection limits (21-4)

6. Types of interference (21-5)

Atomic Spectroscopy

Atomic Absorption

Atomic Fluorescence

Atomic Fluorescence

ICP-MS: Inductively coupled plasma-mass spectrometry

Detection Limit

Detection Limit: Zeeman effect

Interference

1. Spectral interference

2. Chemical interference

3. Ionization interference