UNIVERSITY OF RHODE ISLAND

Department of ChemistryVIRTUAL SEMINAR

3:00 PM, Monday, February 1, 2021

Please email blucht@uri.edu for link

KiBum LeeDepartment of Chemistry and Chemical Biology

Rutgers University

“Bio-inspired Nanotechnology and

Chemical Biology Approached for

Advanced Stem Cell Therapies”

HOST

Brett Lucht

Department of Chemistry

401-874-5071

Presenter: KiBum Lee (Rutgers University) 2021 UIR-CHEM DEPT /ABSTRACT

Bio-inspired Nanotechnology and Chemical Biology Approached for Advanced Stem Cell Therapies

KiBum Lee

Dept. of Chemistry & Chemical Biology, Rutgers University 123 Bevier Road, Piscataway, NJ 08854-8087, USA

This presentation will focus on the interface between nanoscience and stem cell biology. Even though it is well established that stem cell fate is regulated by interactions that occur between microenvironmental cues and intrinsic cellular programs, our understanding of the function of the microenvironment and gene expression in stem cells is hampered by the limitations of conventional methods and the lack of extensive knowledge of multiple regulatory signals.

Addressing the aformentioned challenges, the goal of our recent research program is to develop both approaches from nanotechnology—the “top-down” patterning of extracellular matrix (ECM) and signal molecules (e.g. ECM compositions, nanotopography, pattern geometry, and pattern density), and the “bottom-up” synthesis of multifunctional nanoparticles and their surface modification with specific signal molecules, which can be combined synergistically. Our bottom-up approaches mainly focus on the synthesis and utilization of multifunctional nanoparticles as drug and gene delivery vehicles to manipulate the expression of key genes in stem cells and somatic cells for cellular reprogramming. For example, we recently developed combinatorial nanoarrays of hybrid nanostructures using chemically modified graphenes and Biodegradable Hybrid Nanoscaffolds (BHI), which were further utilized to deliver genetic materials into stem cells for controlling their neural-differentiation pathways and neuronal behaviors in vitro and in vivo [Figure 1.]. In this presentation, a summary of the most updated results from these efforts and future directions will be discussed.

KEY REFERENCES

1. "Effective Modulation of CNS Inhibitory Microenvironment using Bio-inspired Hybrid Nanoscaffold-based Therapeutic Interventions", Advanced Materials, 2020.

2. "4D Printed Transformable Tube Array for High-throughput 3D Cell Culture and Histology", Advanced Materials, 2020.

3. “Sensing Neurotransmitters From Stem Cell-derived Neural Interface at the Single-Cell Level Using Graphene Oxide-Hybrid Nano-SERS Array”, Nano Letter, 2020

4. “NIR Biosensing of Neurotransmitters in Stem Cell-derived Neural Interface Using Advanced Core-shell Upconversion Nanoparticles”, Advanced Materials, 2019.

5. “Engineered Mesenchymal Stem Cell/Nanomedicine Spheroid as an Active Drug Delivery Platform for Combinational Glioblastoma Therapy”, Nano Letters, 2019.

6. "A Biodegradable Hybrid Inorganic Nanoscaffold for Advanced Stem Cell Therapy ", Nature Comm., 2018.

7. “Non-destructive Real-Time Monitoring of Enhanced Stem Cell Differentiation using a Graphene-Au Hybrid Nanoelectrode Array”, Advanced Materials, 2018.

8. “Nanotechnology-based Approaches for Guiding Neural Regeneration”, Accounts of Chemical Research, 2016.

9. "Cyclophilin A promotes cell migration via the Abl-Crk signaling pathway", Nature Chemical Biology, 2016.

10. “Design, Synthesis, and Characterization of Graphene-Nanoparticle Hybrid Materials for Bio-applications”, Chemical Reviews, 2015, 115, 2483-2531.

KEYWORDS: Bio-inspired Hybrid Nanomaterials, Stem Cell Therapeutics, and CNS repair

ACKNOWLEDGEMENTS: The presenter would like to acknowledge KBLEE group members and collaborators.

http://kblee.rutgers.edu/

Fig. 1 A biodegradable hybrid inorganic

(BHI) nanoscaffold for advanced stem cell

therapy. This image illustrates the MnO2

2D nanomaterial-assembled nanoscaffold

for enhanced stem cell transplantation and

improved treatment of murine

hemisection SCI in vivo.