K3 & K3R series Benchtop Centrifuges Instruction Manualcorelifesciences.com/docs/manuals/centurion/K3_Instructions_A4.pdf · K3 & K3R series Benchtop Centrifuges Instruction Manual

K3 & K3R seriesBenchtop Centrifuges

Instruction Manual

Download from www.centurionscientific.co.ukclick ‘K3 Series’

click ‘Manual Instruction Technical’

Deceleration Rate Set

Key to Displays(See pages 5-9 of this manual for programming instructions - a larger copy of this KEY is included in an appendix)

Program

Last time countdown

Open Lid

Last running RCF

Last RCFt

Start

Screen when runningStart Screen

Time counting downRCFt counting up

RCF actual

User name

Time Set

RCFtNot set

RPM orRCF Set

Programmed Rotor Set

Acceleration Rate Set

GENERAL SAFETYTo ensure that the centrifuge is operated in a safe manner, avoiding the hazard of injury to personnel or damage to material goods, the following safety precautions should be followed at all times: •Thecentrifugemustonlybeopenedbytrainedpersonnelcompetentinitsuse •Neverusethecentrifugeunlesstherotorisproperlymountedandsecure •Neveropen,orattempttoopenthelidwhilethecentrifugerotorisstillturning. •Onlyuseoriginalpartsforthecentrifuge •Neveroperatethecentrifugeifcomponentsaremissingordamaged. •Iftherotorshowsvisiblesignsofwearitsusemustbediscontinuedandreplacementfitted. •Therotormustnotbeoverloaded,themaximumalloweddensityofsamplesatfulloperatingspeed is 1.2g/cm3. •Alwaysendeavourtomakesurethatoppositetubesareofthesameweighttoavoidrotorimbalance.Ifthetubesarefilledwiththesamematerialtheymust,therefore,befilledwiththesame amount. •Nochangesshouldbemadetothemechanicalorelectricalcomponentswithoutpriorconsul-tation with and the written permission of Centurion Scientific. •Thecentrifuge’shasnotbeenmanufacturedfrominertmaterialsortobeexplosionproof.Ensurethatitisnotoperatedwithinanenvironment,orwithmaterialsthatmakesthesearequirement. •Duringoperationa“safe”zoneof30cmmustbemaintainedaroundthecentrifuge.Thiszoneshouldbeclearofpersonnelandhazardousmaterialsatalltimesduringtherun. •Substancesofaradioactive,flammableorexplosivenaturemustnotbecentrifuged. •Substancespronetoreactbrisklywitheachothermustnotbecentrifugedatthesametime. •Toxinsandpathogenicmicro-organismsmustnotbecentrifugedunlesstheproperprecau-tionsfortheirhandlinghavebeentaken.Thesemayinclude,butarenotlimitedto,biologicalseals.Should toxins or pathogens enter the centrifuge or its parts the proper procedures for disinfecting thecentrifugeshouldbecarriedout. •Stronglycorrosivesubstancesthatmaycausedamagetoorimpairthemechanicalstrengthoftherotormayonlybecentrifugedinsideprotectivevessels.Shouldanydoubtexistregardingthesubstanceorthesuitabilityofaparticularvesselforusewithit,consulttheSafetyInspector.




Time Set

RCFtNot set

RCF(g)Set

Stop

User name

ActualTemperature(Refrigerated only)

Temp Set(Refrigerated only)

Temp Set(Refrigerated only)

ActualTemperature(Refrigerated only)

Pre-cool /pre-run(Refrigerated only)

2

Centurion Scientific K3 series instructions PAGE2

INSTALLATION

Theinstrumenthasbeendesignedinsuchawaythatinstallationshouldpresentnoproblemstoatechnicianfamiliarwithequipment of this type.ShouldanyproblemsofquestionsariseduringtheinstallationoftheinstrumentpleaserefertoCenturionScientificforadvice.Duringinstallationtherecordfortheindividualcentrifuge(tobefoundwithourmanual)mustbecompleted.

WORK surfaceTheworksurfacemustbestableand,wherepossible,resonancefree–astandardlaboratorybenchisusuallysuitable.Thelocationofthecentrifugeshouldbewellventilatedandoutofdirectsunlight,havingsufficientroomtoallowairtocirculatearounditfreely.Asafetyzoneextendingfor30cminalldirectionsmustbemaintainedaroundthecentrifuge.Thisspaceshouldbekeptclear,inparticularnohazardousmaterialmaybeplaceintheareaduringcentrifugation.

MAINS connectionBeforeconnectingtheunittothemains,ensurethatthevoltageandfrequencyofsupplymatchthespecificationsontheinstrument.Amainsplug,providedwitha5ampfuse(or13ampforrefrigeratedunits)andofatypesuitableforusewherethecentrifugeistobeinstalled,shouldbefitted.Thisplugmustbewiredupaccordingtotheinternationalconvention.

ROTOR assemblyTherotorassemblylocatesontothetaperedshaftofthedriveshaft,beingheldsecurelyinpositionbyaflatwasherandlockingscrew.Itisimportantthattheseitemsareassembledinthecorrectorderandthatthewasherisusedasintended.Tolocatetherotorfirst,checkthatboththeexteriorsurfaceoftheshaftandthetaperedboreoftherotoritselfarefreedirtoranyforeigndebris.Therotorshouldthenbeloweredintopositiononthetaperedshaft,carebeingtakentoensurethatitseatsfirmlyandevenly.Theflatwasherfollowedbythescrewisthenplacedintothethreadedportionoftheshaft,theassemblythenbeingsecuredbythescrew.Thescrewshouldbehandtightenedwiththekeyprovided.

Rotor screw fixing Rotor bolt fixing

3


OPERATIONFILLING and loading of sample tubes.Thecentrifugefunctionsinthemostefficientmannerwhenunbalanceoftherotorisminimised.Thispreventsunduevibrationperturbingtheseparationzonesofthesubstancebeingcentrifuged.Itisthereforeimportantthatunbalanceduetounequalloadingofthesampletubesisminimisedasfaraspossible.Themaximumpermissibleimbalanceoftherotoris1g.Imbalancemaybeminimisedbyfollowingafewsimpleandquickproceduresasdescribedinthefollowingparagraphs.•Thesampletubesshouldbefilledasevenlyaspossible.Wherepractical,tubescontainingthesamesubstanceshouldbeplacedoppositeeachother.•Therotorshouldbeloadedsymmetrically,thisbeingbestachievedbyusingallofthebores.Whenonlypartiallyloadingtherotoritmustbeensuredthatoppositeboresalwaysreceivetubesofthesameweight.Thefollowingpicturesprovideexamplesofcorrectandincorrectloadingoftherotor.

EMERGENCY lid release in case of lid lock failureIntheunlikelyeventofthelidlockfailingandtheuserbeingunabletoremovetheirsamples,anemergencylidreleaseisprovided.WARNING:OPERATIONOFTHEEMERGENCYLIDRELEASEMUSTONLYBEUNDERTAKENBYQUALIFIEDANDAPPROVEDPERSONNEL.UNDERNOCIRCUMSTANCESMAYTHECENTRIFUGEBEOPERATEDINTHECONDITION.Waituntiltherotorhasstoppedturning(viewthroughthelidport)andswitchoffthepowertothecentrifuge.RemovetheblackplasticsealstofrontundersideofcentrifugeandPULLBOTHCORDSDOWN.WARNING:UNDERNOCIRCUMSTANCESMUSTTHEEMERGENCYLIDRELEASEBEUSEDWHILETHECENTRIFUGEISRUNNING.

Cleaning, Disinfecting and MaintenanceWARNING:ISOLATEANDDISCONNECTTHEPOWERSUPPLYBEFORECARRYINGOUTCLEANINGORMAINTENANCE.

CENTRIFUGE BODY•Regularlywipedowntheexternalsurfaceswithamilddetergent.•Mopupanyspillagewithanabsorbentcloth.Checktoensurethattheliquidhasnotenteredthemainbodyofthecentrifuge.WARNING:IfanyliquidhasenteredthemainbodyofthecentrifugeitMUSTNOTbeused.ContactthesupplierorCenturionScientificLtdimmediatelyforadvice.

ROTOR•Removethelockingscrewwiththekeyprovided.•Removetheflatwasher.•Pulltherotorofftheshaftbyapplyingequalforceofbothsides.NOTE:Ifanystiffnessordifficultyisexperiencedwhenattemptingtoremovetherotorthismaybeovercomebyapplyingasharptaptotherotorshaft.Anon-metallicobject(e.g.plasticmallet)mustbeusedtopreventdamagetotheshaft.•Cleantherotorusingasuitabledetergentandhotwater.Alternativelytherotormaybecleaninginanautoclaveatamaximumtemperatureof120oC•Thoroughlyrinseanddrytherotor.•Inspecttherotorforanyhairlinecracks,damageorotherdefects.IfanyarefoundpleasecontactyoursupplierorCenturionScientificLtd.

CORRECT INCORRECT

4


Start point

Display shown at turn on

PRESS program key(bookemblemtopright)

Display shown when program key pressed

PRESS Page number rectangle

Display shown when Page key pressed

SELECT page number required1-9 pages with 12 names per page (a totalof108programscanbestored)

PRESS Set Name KEY in name requiredUpto14characters,letters&numbers(wehavechosenBiochem8fortheseillustrations)

ROTOR PROGRAMMING

Display shown after pressing Tick on name settings

PRESS Set Parameters

Display shown after pressing Set Parameters

PRESS Rotor rectangle

Display shown after pressing Rotor rectangle

FIRST build a program

Display shown when Set Name pressed

SELECT rotor required by pressing down and releasing

continued...

PRESS tick if correct

Display shown when chosen name is being entered

5


Display shown when Time Numerical rectangle is selected

Display shown after selecting rotor

Correct rotor should be shown in Rotor display

Turn end of run buzzer off by PRESSING Bz(Top right of display)

- Turn ON Shows white square in centre

TIME PROGRAMMING

Display shown

PRESS Time rectangle

Display shown when timer rectangle key pressed

RPM PROGRAMMING

Display shown

PRESS RPM square

Display shown when RPM pressed

SELECT RPM (speed) or RCF (G Force)

Display shown after selecting RPM

PRESS Numerical rectangle next to box

SELECT and PRESS desired timer type:

Time 0-9999minutes

Hold Timer is held on till stop buttonispressed

RCFt SeeW2t document for explanation

Pulse Timer counts up in seconds whenSTARTbuttonheld down.(90secondsmax)

PRESS Rectangle next to Time box

SELECT Time required in minutes & secondsHoldandPulsecannotbechangedOrselectRCFtnumberfrompreviousrun

PRESS Tickafter selection

continued...


6

Display shown after pressing numerical rectangle

SET Speed (RPM) or RCF in numerical form

ACCELERATION and DECELERATION ratesSET Acceleration rate

SELECT Rate 1-10(1isslowest10fastest)

Select rate 1-10(1isoff10fullbraking)

Display shown when Acc pressed

PRESS number required & release

Display after selecting AccSET Deceleration (Brake) rate

PRESS Tick after selection.

Display

If you are satisfied with all parametersCheck Rotor, timer type, RPM or RCFAcc & Dec rates

Display

PRESS number required & release

Display shown when Dec pressed

Then PRESS Tick box (bottomright)

PRESS Acc Square

PRESS Dec Square

Display after selecting Acc and Dec rates

forREFRIGERATED MODELS onlySET Temperature - SEE following page 7A, thenreturntosetAccelerationandDecelerationratesbelow


7continued page 8...

7A

Refrigerated Centrifuges onlyK2015R,K241R and K243RCenturionScientificrefrigeratedcentrifugeshavearangeof- 9oC to 40oC.

Weaccuratelycontrolthistemperaturebyrunningtherefrigerationunitandpulsingheatviaaloopclosetothecoils.PID system is used to control.

Imagine a bath at home, to control the temperature accurately you would run both hot and cold taps together. This is far more effective than filling with cold water and topping up with hot as the temperature difference would be enormous.

Byusingthismethodourchambertemperaturecontrolisunsurpassed.

NoteDuetotheefficientmanagementoftemperaturecontrolyoumayseesomeicepatchesorinturn,noticesomeslightlyhotterareas.Thisisquitenormalanditistheoverallchambertemperaturethatmatters.

ForaccuratetemperaturecontrolwerecommendyouusethePre-cool/pre-runfacility .FityourdesiredrotorandsecurelyfastenasperourInstructions.Afterprogrammingyourdesiredparameters,IEselectionofarotor,speed,time,acceleration&decelerationrates,(asanexample).Leavethecentrifugetoreachthedesiredtemperatureandthenpressthe Pre-coolsymbol.Thiswillrunthecentrifugeat20%ofthemaximumspeedoftherotorfor20minutes,a willshowintheRpmsectiontoshowthePre-coolmodeisinoperation.Itisadvisabletoletthetimerunto20minutes,butyoucanstopatanytime.Oncestoppedloadyoursamples(whichshouldbeatyoursettemperature)andpressthestartarrowbutton.

Foreaseitisadvisabletosetyoursamplestothedesiredcentrifugetemperaturebeforeplacinginthecentrifugeandseparating,otherwiseallowsufficienttimeforthemtoreachthetemperatureinthechamber.

TEMPERATURE PROGRAMMING(Refigeratedmodelsonly-K2015R,K241RandK243R)

Display shown K2015R,K241R and K243R(all our illustrated screens show a chosen and actual temperature of 4oC)

PRESS Temperature numeric square

Display shown when Temp is pressed

SET Temp (-9oC...40oC) in numerical form

return to page 7 to set Acceleration and Deceleration rates...

Centurion Scientific K3 series instructions PAGE7A-applicabletoRefigeratedModelsOnly-

PRESS Tick after selection.

Note: See rotor appendix for minimum possible temperature at maximum speed per rotor.

Note: Displays illustrated elswhere in this manual do not show the Temp square or the Temperature numeric square however they will be present on all refigerated model displays as will set temperature and actual temperature where appropriate (see Key to Displays on page 1)

Pre-cool /pre-run START button.Seeinstructionsabovedetailinguse of this feature - note that this screen will show your selected parameters.

Note:Centurion K3 range centrifuges can storeupto108programs,theseareconvienientlygroupedinto9pageswithupto12namesavailableperpage,eachnamecanconsistofupto14alphanumericcharacters.

HOW TO RETRIEVE SET PROGRAMS

NOW LOAD THE CENTRIFUGE

Display

PRESS Central button on right to open lidLOAD your samples evenly(seeSafetyInstructions,page2)

FIT LID to rotor (if part of rotor type)CLOSE Centrifuge lid pressing both sides down firmly.PRESS Start button (arrowtriangle,bottomright)

Display showing running screen

RUNNING screen displayIf you require early terminationTime,Hold,RCFtPRESSSquarebottomright.

OR in pulse mode RELEASEwhencounter(seconds)hasreacheddesirednumber

Run Complete

Display showing screen when run finished (with Buzzer selected)

PRESS run complete Tick(onlyshowsIFBuzzerisselected)

PRESS lid open button(centre right)

You may now retrieve your samples

Display

PRESS program button(top right)

PRESS Pagenumerical box(9 pages available)

PRESS & SELECTrequired name then

PRESS desired name(12 names availble)

your stored program will now be retrieved.


8

If Display shows Rotor out of balanceAnimbalancehasoccurred

If Display shows Tacho failurePRESS Tick buttonPRESS Lid open buttonCheck rotor for free rotationArethetubestootall?

CALL service department

If Display showsLid Open!Check lid is closed correctly

To check sensors go to parameters page.FirstmakesureCentrifugelidisopen.Press‘di’(toprightsmallbutton)forDiagnosticsscreen.(Shown below)Itreadsasfollows: (either 0 or 1 may show when this screen first appears, follow instructions below to perform diagnostic checks)

When centrifuge has stopped PRESS tick buttonPRESS Lid open buttonCheckfor:Improperlyloadedtubes(see Safety pages)Brokentubesorincorrectlymeasuredtube

Error code shown on display

Error code shown on display

ERROR CODES ON DISPLAYCenturion Scientific K3 series instructions PAGE9

PRESS down firmly on lid (atbothsidesnearfront)

IfnotthenCHECKSENSORasbelowOrCALLServiceDepartment

.... or greater (version number of installed firmware)

.....0or1 .....ByrotatingrotorTachowillshow0or1asrotorturnsifsoTachometerisworking

.....0or1 .....ByrotatingrotorRotorRecwillshow0or1asrotorturnsifsosensorisworking

.....0or1 .....BypressingrotorfirmlyorshakinghardBalancewillshow0or1

.....0or1 .....ByshuttingthelidtheLidswitchwillgofrom0to1showingcorrectsensing

Lid Open! Error code shown on display

CenturionScientificLtd-AllCenturionequipmentisdesignedandmanufacturedunderstrictQualityassuranceconditionsandrigoroustestingandthereforeshouldprovideyouwithyearsoftroublefreeservice.However,shouldyourequirerepairsormaintenanceworkonyourCentrifugepleasecontactourservicedepartmenton+44(0)2392631225.FornoneUKcustomers,pleasecontactyourlocalCenturiondistributorforservice,maintenanceandaftersalessupport.

9

Centurion Scientific K3 series instructions Appendix

Key to Displays(Seepreviouspagesforprogramminginstructions)

Time Set


RCFt(Not set)

RPMor

RCFSet



User name Program

Last timecountdown

Open Lid

Last running RPM

Start

Last RCFt

Start Screen

Screen when running

Time counting down

RCFt counting up

RCF actual




Time Set

RCFt(Not set)

RCF(g)Set

Stop

User name

ActualTemperature(Refrigeratedonly)Temp Set

(Refrigeratedonly)

Time Set


RCFt(Not set)

RPMor

RCFSet



User name Program

Last timecountdown

Open Lid

Last running RPM

Start

Last RCFt

Start ScreenRefrigerated models only

ActualTemperature(Refrigeratedonly)

Temp Set(Refrigerated

only)

Documents

K3 & K3R series Benchtop Centrifuges Instruction Manualcorelifesciences.com/docs/manuals/centurion/K3_Instructions_A4.pdf · K3 & K3R series Benchtop Centrifuges Instruction Manual